LOCUS       DAEEBB010000001        88079 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEEBB010000001 DAEEBB010000000
VERSION     DAEEBB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.001"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="KLF07_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="KLF07_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBD9830146.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="KLF07_000002"
     tRNA            1230..1305
                     /locus_tag="KLF07_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="KLF07_000003"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="KLF07_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="KLF07_000004"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="KLF07_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBD9830147.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="KLF07_000005"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="KLF07_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBD9830148.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="KLF07_000006"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="KLF07_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBD9830149.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="KLF07_000007"
     CDS             complement(4869..5306)
                     /locus_tag="KLF07_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBD9830150.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="KLF07_000008"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="KLF07_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="KLF07_000009"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="KLF07_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBD9830151.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="KLF07_000010"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="KLF07_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBD9830152.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="KLF07_000011"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="KLF07_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBD9830153.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="KLF07_000012"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="KLF07_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBD9830154.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="KLF07_000013"
     CDS             10774..11442
                     /locus_tag="KLF07_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBD9830155.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="KLF07_000014"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="KLF07_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBD9830156.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="KLF07_000015"
     CDS             11985..13817
                     /locus_tag="KLF07_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBD9830157.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="KLF07_000016"
     CDS             13807..14157
                     /locus_tag="KLF07_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HBD9830158.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="KLF07_000017"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="KLF07_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBD9830159.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="KLF07_000018"
     CDS             14476..14898
                     /locus_tag="KLF07_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBD9830160.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="KLF07_000019"
     CDS             14901..15533
                     /locus_tag="KLF07_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBD9830161.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="KLF07_000020"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="KLF07_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBD9830162.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="KLF07_000021"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="KLF07_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBD9830163.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="KLF07_000022"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="KLF07_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBD9830164.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="KLF07_000023"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="KLF07_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="KLF07_000024"
     CDS             18712..19824
                     /locus_tag="KLF07_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBD9830165.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="KLF07_000025"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="KLF07_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="KLF07_000026"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="KLF07_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBD9830166.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="KLF07_000027"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="KLF07_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBD9830167.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /locus_tag="KLF07_000028"
     CDS             22078..22521
                     /locus_tag="KLF07_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBD9830168.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="KLF07_000029"
     CDS             complement(22622..22951)
                     /locus_tag="KLF07_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBD9830169.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="KLF07_000030"
     CDS             complement(23123..24181)
                     /locus_tag="KLF07_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD9830170.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="KLF07_000031"
     CDS             complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="KLF07_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBD9830171.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="KLF07_000032"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="KLF07_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBD9830172.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="KLF07_000033"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="KLF07_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBD9830173.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /locus_tag="KLF07_000034"
     CDS             complement(27816..28043)
                     /locus_tag="KLF07_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBD9830174.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="KLF07_000035"
     CDS             complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="KLF07_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBD9830175.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="KLF07_000036"
     CDS             complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="KLF07_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBD9830176.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="KLF07_000037"
     CDS             complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="KLF07_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBD9830177.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30919..31848)
                     /locus_tag="KLF07_000038"
     CDS             complement(30919..31848)
                     /locus_tag="KLF07_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9830178.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31894..32718)
                     /locus_tag="KLF07_000039"
     CDS             complement(31894..32718)
                     /locus_tag="KLF07_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD9830179.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="KLF07_000040"
     CDS             complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="KLF07_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBD9830180.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="KLF07_000041"
     CDS             complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="KLF07_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBD9830181.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33586..33636
                     /gene="hisL"
                     /locus_tag="KLF07_000042"
     CDS             33586..33636
                     /gene="hisL"
                     /locus_tag="KLF07_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBD9830182.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33612..33735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33782..34681
                     /gene="hisG"
                     /locus_tag="KLF07_000043"
     CDS             33782..34681
                     /gene="hisG"
                     /locus_tag="KLF07_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBD9830183.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34687..35991
                     /gene="hisD"
                     /locus_tag="KLF07_000044"
     CDS             34687..35991
                     /gene="hisD"
                     /locus_tag="KLF07_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBD9830184.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35988..37058
                     /gene="hisC"
                     /locus_tag="KLF07_000045"
     CDS             35988..37058
                     /gene="hisC"
                     /locus_tag="KLF07_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBD9830185.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37058..38125
                     /gene="hisB"
                     /locus_tag="KLF07_000046"
     CDS             37058..38125
                     /gene="hisB"
                     /locus_tag="KLF07_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBD9830186.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38125..38715
                     /gene="hisH"
                     /locus_tag="KLF07_000047"
     CDS             38125..38715
                     /gene="hisH"
                     /locus_tag="KLF07_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBD9830187.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38715..39452
                     /gene="hisA"
                     /locus_tag="KLF07_000048"
     CDS             38715..39452
                     /gene="hisA"
                     /locus_tag="KLF07_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBD9830188.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39434..40210
                     /gene="hisF"
                     /locus_tag="KLF07_000049"
     CDS             39434..40210
                     /gene="hisF"
                     /locus_tag="KLF07_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBD9830189.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40204..40815
                     /locus_tag="KLF07_000050"
     CDS             40204..40815
                     /locus_tag="KLF07_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBD9830190.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="KLF07_000051"
     CDS             complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="KLF07_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBD9830191.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="KLF07_000052"
     CDS             complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="KLF07_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBD9830192.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="KLF07_000053"
     CDS             complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="KLF07_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBD9830193.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="KLF07_000054"
                     /gene_synonym="manB"
     CDS             complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="KLF07_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBD9830194.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="KLF07_000055"
     CDS             complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="KLF07_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBD9830195.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="KLF07_000056"
     CDS             complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="KLF07_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBD9830196.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="KLF07_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49155..49634)
                     /gene="gmm"
                     /locus_tag="KLF07_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBD9830197.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="KLF07_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="KLF07_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBD9830198.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="KLF07_000059"
     CDS             complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="KLF07_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBD9830199.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="KLF07_000060"
     CDS             complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="KLF07_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBD9830200.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="KLF07_000061"
     CDS             complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="KLF07_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBD9830201.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="KLF07_000062"
     CDS             complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="KLF07_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBD9830202.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="KLF07_000063"
     CDS             complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="KLF07_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBD9830203.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="KLF07_000064"
     CDS             complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="KLF07_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBD9830204.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="KLF07_000065"
     CDS             complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="KLF07_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBD9830205.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="KLF07_000066"
     CDS             complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="KLF07_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBD9830206.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="KLF07_000067"
     CDS             complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="KLF07_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBD9830207.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59516..60655)
                     /gene="wza"
                     /locus_tag="KLF07_000068"
     CDS             complement(59516..60655)
                     /gene="wza"
                     /locus_tag="KLF07_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBD9830208.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60964..61113
                     /locus_tag="KLF07_000069"
     CDS             60964..61113
                     /locus_tag="KLF07_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830209.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61314..62897
                     /locus_tag="KLF07_000070"
     CDS             61314..62897
                     /locus_tag="KLF07_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD9830210.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63171..65024)
                     /gene="asmA"
                     /locus_tag="KLF07_000071"
     CDS             complement(63171..65024)
                     /gene="asmA"
                     /locus_tag="KLF07_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBD9830211.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="KLF07_000072"
     CDS             complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="KLF07_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBD9830212.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65719..66360)
                     /gene="udk"
                     /locus_tag="KLF07_000073"
     CDS             complement(65719..66360)
                     /gene="udk"
                     /locus_tag="KLF07_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBD9830213.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66678..69995
                     /locus_tag="KLF07_000074"
     CDS             66678..69995
                     /locus_tag="KLF07_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD9830214.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="KLF07_000075"
     CDS             complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="KLF07_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBD9830215.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71087..72439
                     /gene="yegD"
                     /locus_tag="KLF07_000076"
     CDS             71087..72439
                     /gene="yegD"
                     /locus_tag="KLF07_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD9830216.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="KLF07_000077"
     CDS             complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="KLF07_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBD9830217.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="KLF07_000078"
     CDS             complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="KLF07_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBD9830218.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75147..75806)
                     /locus_tag="KLF07_000079"
     CDS             complement(75147..75806)
                     /locus_tag="KLF07_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD9830219.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="KLF07_000080"
     CDS             complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="KLF07_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBD9830220.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76359..76415)
                     /locus_tag="KLF07_000081"
     CDS             complement(76359..76415)
                     /locus_tag="KLF07_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD9830221.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76686..76742)
                     /locus_tag="KLF07_000082"
     CDS             complement(76686..76742)
                     /locus_tag="KLF07_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD9830222.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77021..78262
                     /gene="mdtA"
                     /locus_tag="KLF07_000083"
     CDS             77021..78262
                     /gene="mdtA"
                     /locus_tag="KLF07_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBD9830223.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78262..81384
                     /gene="mdtB"
                     /locus_tag="KLF07_000084"
     CDS             78262..81384
                     /gene="mdtB"
                     /locus_tag="KLF07_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBD9830224.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81385..84462
                     /gene="mdtC"
                     /locus_tag="KLF07_000085"
     CDS             81385..84462
                     /gene="mdtC"
                     /locus_tag="KLF07_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBD9830225.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84463..85878
                     /locus_tag="KLF07_000086"
     CDS             84463..85878
                     /locus_tag="KLF07_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9830226.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85875..87278
                     /gene="baeS"
                     /locus_tag="KLF07_000087"
     CDS             85875..87278
                     /gene="baeS"
                     /locus_tag="KLF07_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBD9830227.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87275..87997
                     /gene="baeR"
                     /locus_tag="KLF07_000088"
     CDS             87275..87997
                     /gene="baeR"
                     /locus_tag="KLF07_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBD9830228.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21561 a        23315 c        22148 g        21055 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatc gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctgaaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    29221 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    29281 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    29341 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    29401 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    29461 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    29521 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    29581 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    29641 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    29701 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    29761 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    29821 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    29881 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    29941 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    30001 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    30061 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    30121 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    30181 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    30241 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    30301 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    30361 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    30421 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    30481 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    30541 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    30601 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    30661 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    30721 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    30781 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    30841 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    30901 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    30961 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    31021 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    31081 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    31141 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    31201 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    31261 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    31321 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    31381 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    31441 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    31501 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    31561 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    31621 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    31681 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    31741 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    31801 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    31861 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    31921 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    31981 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    32041 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    32101 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    32161 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    32221 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    32281 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    32341 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    32401 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    32461 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    32521 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    32581 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    32641 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    32701 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    32761 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    32821 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    32881 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    32941 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    33001 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    33061 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    33121 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    33181 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    33241 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    33301 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    33361 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    33421 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    33481 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    33541 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    33601 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    33661 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    33721 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    33781 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    33841 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    33901 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    33961 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    34021 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    34081 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    34141 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    34201 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    34261 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    34321 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    34381 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    34441 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    34501 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    34561 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    34621 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    34681 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    34741 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    34801 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    34861 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    34921 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    34981 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    35041 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    35101 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    35161 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    35221 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    35281 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    35341 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    35401 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    35461 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    35521 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    35581 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    35641 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    35701 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    35761 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    35821 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    35881 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    35941 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    36001 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    36061 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    36121 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    36181 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    36241 acgaaggtat tgaactgctg attcgcgctt tttgcgaatc gggtaaagac gccatcctct
    36301 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    36361 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    36421 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    36481 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    36541 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    36601 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    36661 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    36721 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    36781 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    36841 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    36901 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    36961 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    37021 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    37081 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    37141 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    37201 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    37261 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    37321 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    37381 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    37441 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    37501 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    37561 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    37621 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    37681 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    37741 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    37801 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    37861 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    37921 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    37981 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    38041 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    38101 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    38161 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    38221 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    38281 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    38341 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    38401 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    38461 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    38521 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    38581 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    38641 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    38701 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    38761 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    38821 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    38881 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    38941 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    39001 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    39061 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    39121 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    39181 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    39241 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    39301 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    39361 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    39421 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    39481 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    39541 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    39601 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    39661 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    39721 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    39781 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    39841 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    39901 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    39961 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    40021 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    40081 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    40141 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    40201 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    40261 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    40321 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    40381 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    40441 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    40501 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    40561 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    40621 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    40681 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    40741 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    40801 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    40861 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    40921 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    40981 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    41041 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    41101 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    41161 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    41221 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    41281 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    41341 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    41401 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    41461 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    41521 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    41581 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    41641 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    41701 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    41761 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcgctag
    41821 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    41881 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    41941 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    42001 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    42061 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    42121 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    42181 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    42241 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    42301 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    42361 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    42421 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    42481 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    42541 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    42601 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    42661 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    42721 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    42781 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    42841 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    42901 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    42961 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    43021 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    43081 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    43141 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    43201 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    43261 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    43321 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    43381 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    43441 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    43501 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    43561 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    43621 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    43681 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    43741 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    43801 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    43861 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    43921 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    43981 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    44041 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    44101 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    44161 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    44221 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    44281 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    44341 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    44401 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    44461 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    44521 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    44581 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    44641 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    44701 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    44761 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    44821 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    44881 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    44941 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    45001 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    45061 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    45121 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    45181 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    45241 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    45301 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    45361 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    45421 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    45481 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    45541 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    45601 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    45661 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    45721 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    45781 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    45841 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    45901 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    45961 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    46021 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    46081 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    46141 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    46201 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    46261 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    46321 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    46381 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    46441 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    46501 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    46561 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    46621 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    46681 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    46741 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    46801 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    46861 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    46921 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    46981 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    47041 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    47101 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    47161 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    47221 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    47281 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    47341 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    47401 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    47461 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    47521 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    47581 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    47641 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    47701 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    47761 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    47821 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    47881 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    47941 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    48001 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    48061 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    48121 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    48181 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    48241 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    48301 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    48361 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    48421 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    48481 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    48541 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    48601 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    48661 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    48721 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    48781 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    48841 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    48901 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    48961 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    49021 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    49081 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    49141 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    49201 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    49261 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    49321 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    49381 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    49441 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    49501 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    49561 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    49621 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    49681 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    49741 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    49801 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    49861 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    49921 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    49981 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    50041 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    50101 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    50161 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    50221 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    50281 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    50341 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    50401 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    50461 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    50521 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    50581 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    50641 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    50701 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    50761 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    50821 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    50881 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    50941 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    51001 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    51061 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    51121 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    51181 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    51241 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    51301 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    51361 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    51421 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    51481 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    51541 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    51601 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    51661 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    51721 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    51781 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    51841 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    51901 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    51961 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    52021 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    52081 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    52141 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    52201 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    52261 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    52321 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    52381 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    52441 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    52501 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    52561 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    52621 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    52681 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    52741 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    52801 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    52861 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    52921 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    52981 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    53041 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    53101 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    53161 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    53221 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    53281 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    53341 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    53401 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    53461 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    53521 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    53581 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    53641 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    53701 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    53761 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    53821 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    53881 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    53941 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    54001 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    54061 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    54121 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    54181 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    54241 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    54301 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    54361 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    54421 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    54481 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    54541 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    54601 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    54661 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    54721 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    54781 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    54841 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    54901 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    54961 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    55021 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    55081 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    55141 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    55201 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    55261 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    55321 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    55381 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    55441 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    55501 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    55561 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    55621 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    55681 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    55741 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    55801 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    55861 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    55921 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    55981 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    56041 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    56101 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    56161 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    56221 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    56281 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    56341 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    56401 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    56461 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    56521 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    56581 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    56641 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    56701 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    56761 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    56821 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    56881 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    56941 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    57001 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    57061 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    57121 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    57181 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    57241 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    57301 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    57361 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    57421 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    57481 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    57541 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    57601 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    57661 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    57721 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    57781 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    57841 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    57901 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    57961 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    58021 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    58081 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    58141 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    58201 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    58261 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    58321 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    58381 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    58441 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    58501 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    58561 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    58621 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    58681 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    58741 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    58801 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    58861 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    58921 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    58981 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    59041 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    59101 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    59161 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    59221 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    59281 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    59341 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    59401 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    59461 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    59521 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    59581 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    59641 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    59701 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    59761 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    59821 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    59881 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    59941 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    60001 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    60061 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    60121 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    60181 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    60241 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    60301 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    60361 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    60421 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    60481 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    60541 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    60601 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    60661 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    60721 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    60781 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    60841 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    60901 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    60961 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    61021 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    61081 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    61141 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    61201 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    61261 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    61321 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    61381 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    61441 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    61501 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    61561 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    61621 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    61681 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    61741 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    61801 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    61861 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    61921 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    61981 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    62041 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    62101 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    62161 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    62221 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    62281 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    62341 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    62401 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    62461 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    62521 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    62581 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    62641 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    62701 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    62761 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    62821 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    62881 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    62941 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    63001 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    63061 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    63121 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac ctacatcttc
    63181 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    63241 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    63301 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    63361 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    63421 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    63481 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    63541 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    63601 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    63661 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    63721 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    63781 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    63841 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    63901 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    63961 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    64021 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    64081 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    64141 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    64201 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    64261 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    64321 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    64381 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    64441 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    64501 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    64561 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    64621 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    64681 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    64741 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    64801 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    64861 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    64921 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    64981 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    65041 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    65101 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    65161 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    65221 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    65281 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    65341 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    65401 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    65461 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    65521 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    65581 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    65641 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    65701 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    65761 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    65821 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    65881 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    65941 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    66001 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    66061 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    66121 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    66181 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    66241 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    66301 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    66361 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    66421 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    66481 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    66541 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    66601 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    66661 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    66721 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    66781 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    66841 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    66901 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    66961 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    67021 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    67081 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    67141 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    67201 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    67261 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    67321 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    67381 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    67441 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    67501 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    67561 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    67621 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    67681 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    67741 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    67801 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    67861 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    67921 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    67981 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    68041 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    68101 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    68161 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    68221 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    68281 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    68341 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    68401 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    68461 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    68521 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    68581 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    68641 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    68701 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    68761 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    68821 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    68881 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    68941 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    69001 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    69061 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    69121 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    69181 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    69241 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    69301 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    69361 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    69421 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    69481 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    69541 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    69601 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    69661 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    69721 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    69781 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    69841 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    69901 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    69961 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    70021 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    70081 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    70141 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    70201 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    70261 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    70321 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    70381 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    70441 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    70501 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    70561 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    70621 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    70681 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    70741 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    70801 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    70861 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    70921 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    70981 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    71041 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    71101 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    71161 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    71221 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    71281 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    71341 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    71401 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    71461 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    71521 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    71581 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    71641 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    71701 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    71761 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    71821 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    71881 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    71941 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    72001 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    72061 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    72121 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    72181 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    72241 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    72301 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    72361 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    72421 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    72481 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    72541 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    72601 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    72661 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    72721 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    72781 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    72841 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    72901 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    72961 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    73021 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    73081 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    73141 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    73201 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    73261 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    73321 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    73381 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    73441 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    73501 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    73561 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    73621 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    73681 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    73741 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    73801 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    73861 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    73921 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    73981 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    74041 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    74101 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    74161 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    74221 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    74281 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    74341 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    74401 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    74461 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    74521 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    74581 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    74641 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    74701 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    74761 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    74821 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    74881 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    74941 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    75001 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    75061 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    75121 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    75181 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    75241 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    75301 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    75361 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    75421 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    75481 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    75541 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    75601 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    75661 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    75721 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    75781 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    75841 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    75901 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    75961 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    76021 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    76081 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    76141 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    76201 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    76261 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    76321 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    76381 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    76441 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    76501 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    76561 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    76621 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    76681 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    76741 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    76801 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    76861 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    76921 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    76981 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    77041 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    77101 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    77161 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    77221 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    77281 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    77341 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    77401 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    77461 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    77521 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    77581 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    77641 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    77701 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    77761 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    77821 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    77881 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    77941 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    78001 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    78061 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    78121 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    78181 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    78241 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    78301 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    78361 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    78421 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    78481 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    78541 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    78601 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    78661 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    78721 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    78781 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    78841 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    78901 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    78961 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    79021 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    79081 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    79141 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    79201 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    79261 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    79321 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    79381 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    79441 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    79501 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    79561 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    79621 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    79681 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    79741 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    79801 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    79861 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    79921 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    79981 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    80041 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    80101 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    80161 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    80221 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    80281 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    80341 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    80401 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    80461 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    80521 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    80581 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    80641 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    80701 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    80761 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    80821 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    80881 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    80941 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    81001 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    81061 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    81121 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    81181 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    81241 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    81301 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    81361 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    81421 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    81481 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    81541 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    81601 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    81661 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    81721 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    81781 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    81841 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    81901 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    81961 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    82021 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    82081 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    82141 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    82201 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    82261 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    82321 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    82381 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    82441 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    82501 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    82561 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    82621 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    82681 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    82741 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    82801 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    82861 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    82921 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    82981 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    83041 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    83101 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    83161 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    83221 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    83281 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    83341 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    83401 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    83461 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    83521 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    83581 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    83641 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    83701 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    83761 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    83821 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    83881 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    83941 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    84001 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    84061 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    84121 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    84181 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    84241 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    84301 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    84361 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    84421 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    84481 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    84541 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    84601 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    84661 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    84721 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    84781 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    84841 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    84901 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    84961 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    85021 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    85081 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    85141 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    85201 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    85261 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    85321 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    85381 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    85441 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    85501 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    85561 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    85621 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    85681 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    85741 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    85801 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    85861 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    85921 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    85981 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    86041 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    86101 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    86161 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    86221 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    86281 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    86341 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    86401 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    86461 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    86521 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    86581 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    86641 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    86701 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    86761 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    86821 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    86881 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    86941 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    87001 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    87061 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    87121 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    87181 cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    87241 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    87301 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    87361 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    87421 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    87481 cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc
    87541 aaaaatcgaa gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg
    87601 caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    87661 accgcagcgc gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    87721 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt
    87781 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    87841 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    87901 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    87961 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag
    88021 ccggagatgg cgttcagacc agatccggca acattatccc acgcatggtc agcaaactg
//
LOCUS       DAEEBB010000002        79578 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEEBB010000002 DAEEBB010000000
VERSION     DAEEBB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.002"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="KLF07_000089"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="KLF07_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="KLF07_000090"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="KLF07_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBD9830229.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="KLF07_000091"
     CDS             2354..2758
                     /locus_tag="KLF07_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBD9830230.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="KLF07_000092"
     CDS             2811..2921
                     /locus_tag="KLF07_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830231.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="KLF07_000093"
     CDS             3157..3201
                     /locus_tag="KLF07_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830232.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="KLF07_000094"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="KLF07_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBD9830233.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="KLF07_000095"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="KLF07_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBD9830234.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="KLF07_000096"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="KLF07_000096"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBD9830235.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="KLF07_000097"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="KLF07_000097"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBD9830236.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="KLF07_000098"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="KLF07_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBD9830237.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="KLF07_000099"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="KLF07_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBD9830238.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="KLF07_000100"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="KLF07_000100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBD9830239.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="KLF07_000101"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="KLF07_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBD9830240.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="KLF07_000102"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="KLF07_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBD9830241.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="KLF07_000103"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="KLF07_000103"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBD9830242.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="KLF07_000104"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="KLF07_000104"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBD9830243.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="KLF07_000105"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="KLF07_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBD9830244.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="KLF07_000106"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="KLF07_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBD9830245.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="KLF07_000107"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="KLF07_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBD9830246.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="KLF07_000108"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="KLF07_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBD9830247.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="KLF07_000109"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="KLF07_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBD9830248.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="KLF07_000110"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="KLF07_000110"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBD9830249.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="KLF07_000111"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="KLF07_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBD9830250.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="KLF07_000112"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="KLF07_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBD9830251.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="KLF07_000113"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="KLF07_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBD9830252.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="KLF07_000114"
     CDS             23801..24874
                     /locus_tag="KLF07_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBD9830253.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="KLF07_000115"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="KLF07_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBD9830254.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="KLF07_000116"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="KLF07_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="KLF07_000117"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="KLF07_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBD9830255.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="KLF07_000118"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="KLF07_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBD9830256.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="KLF07_000119"
     CDS             complement(30823..31362)
                     /locus_tag="KLF07_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD9830257.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="KLF07_000120"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="KLF07_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBD9830258.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="KLF07_000121"
     CDS             complement(33301..33843)
                     /locus_tag="KLF07_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD9830259.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="KLF07_000122"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="KLF07_000122"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBD9830260.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="KLF07_000123"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="KLF07_000123"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBD9830261.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="KLF07_000124"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="KLF07_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBD9830262.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="KLF07_000125"
     CDS             complement(36362..36739)
                     /locus_tag="KLF07_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBD9830263.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="KLF07_000126"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="KLF07_000126"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBD9830264.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="KLF07_000127"
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="KLF07_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBD9830265.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="KLF07_000128"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="KLF07_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBD9830266.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="KLF07_000129"
     CDS             complement(41412..42209)
                     /locus_tag="KLF07_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD9830267.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="KLF07_000130"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="KLF07_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBD9830268.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="KLF07_000131"
     CDS             complement(43221..43862)
                     /locus_tag="KLF07_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBD9830269.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="KLF07_000132"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="KLF07_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBD9830270.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="KLF07_000133"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="KLF07_000133"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBD9830271.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="KLF07_000134"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="KLF07_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBD9830272.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="KLF07_000135"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="KLF07_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD9830273.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="KLF07_000136"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="KLF07_000136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBD9830274.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="KLF07_000137"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="KLF07_000137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBD9830275.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="KLF07_000138"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="KLF07_000138"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBD9830276.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="KLF07_000139"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="KLF07_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBD9830277.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="KLF07_000140"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="KLF07_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBD9830278.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="KLF07_000141"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="KLF07_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBD9830279.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="KLF07_000142"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="KLF07_000142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBD9830280.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="KLF07_000143"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="KLF07_000143"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBD9830281.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="KLF07_000144"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="KLF07_000144"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBD9830282.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="KLF07_000145"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="KLF07_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBD9830283.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="KLF07_000146"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="KLF07_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBD9830284.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="KLF07_000147"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="KLF07_000147"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBD9830285.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="KLF07_000148"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="KLF07_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBD9830286.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="KLF07_000149"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="KLF07_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBD9830287.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="KLF07_000150"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="KLF07_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="KLF07_000151"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="KLF07_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBD9830288.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="KLF07_000152"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="KLF07_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBD9830289.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="KLF07_000153"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="KLF07_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBD9830290.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="KLF07_000154"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="KLF07_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBD9830291.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="KLF07_000155"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="KLF07_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBD9830292.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="KLF07_000156"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="KLF07_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBD9830293.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="KLF07_000157"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="KLF07_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBD9830294.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="KLF07_000158"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="KLF07_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBD9830295.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="KLF07_000159"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="KLF07_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBD9830296.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="KLF07_000160"
     CDS             complement(71243..72166)
                     /locus_tag="KLF07_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD9830297.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="KLF07_000161"
     CDS             complement(72168..72815)
                     /locus_tag="KLF07_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBD9830298.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="KLF07_000162"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="KLF07_000162"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBD9830299.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="KLF07_000163"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="KLF07_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBD9830300.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="KLF07_000164"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="KLF07_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBD9830301.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="KLF07_000165"
     CDS             75699..76259
                     /locus_tag="KLF07_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD9830302.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="KLF07_000166"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="KLF07_000166"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBD9830303.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="KLF07_000167"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="KLF07_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBD9830304.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="KLF07_000168"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="KLF07_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBD9830305.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="KLF07_000169"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="KLF07_000169"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBD9830306.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19594 a        20914 c        19711 g        19359 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc cttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacatt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actattgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gagcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt gacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagccca gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcatcg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctc
//
LOCUS       DAEEBB010000003        74367 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEEBB010000003 DAEEBB010000000
VERSION     DAEEBB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.003"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            71..1015
                     /locus_tag="KLF07_000170"
                     /pseudo
     CDS             71..1015
                     /locus_tag="KLF07_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="KLF07_000171"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="KLF07_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBD9830307.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="KLF07_000172"
     CDS             1797..1904
                     /locus_tag="KLF07_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBD9830308.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="KLF07_000173"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="KLF07_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBD9830309.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="KLF07_000174"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="KLF07_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBD9830310.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="KLF07_000175"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="KLF07_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBD9830311.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="KLF07_000176"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="KLF07_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBD9830312.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="KLF07_000177"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="KLF07_000177"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBD9830313.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="KLF07_000178"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="KLF07_000178"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD9830314.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="KLF07_000179"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="KLF07_000179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBD9830315.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="KLF07_000180"
     CDS             complement(11226..11813)
                     /locus_tag="KLF07_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBD9830316.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="KLF07_000181"
     CDS             11983..12561
                     /locus_tag="KLF07_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD9830317.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="KLF07_000182"
     CDS             complement(12691..13452)
                     /locus_tag="KLF07_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD9830318.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="KLF07_000183"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="KLF07_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBD9830319.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="KLF07_000184"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="KLF07_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBD9830320.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="KLF07_000185"
     CDS             complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="KLF07_000185"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBD9830321.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16807..17205
                     /locus_tag="KLF07_000186"
     CDS             16807..17205
                     /locus_tag="KLF07_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBD9830322.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17202..17897
                     /locus_tag="KLF07_000187"
     CDS             17202..17897
                     /locus_tag="KLF07_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBD9830323.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            18027..18911
                     /gene="cdd"
                     /locus_tag="KLF07_000188"
     CDS             18027..18911
                     /gene="cdd"
                     /locus_tag="KLF07_000188"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBD9830324.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            19061..19780
                     /gene="sanA"
                     /locus_tag="KLF07_000189"
     CDS             19061..19780
                     /gene="sanA"
                     /locus_tag="KLF07_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBD9830325.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19783..20022
                     /locus_tag="KLF07_000190"
     CDS             19783..20022
                     /locus_tag="KLF07_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBD9830326.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20216..21454
                     /gene="preT"
                     /locus_tag="KLF07_000191"
                     /pseudo
     CDS             20216..21454
                     /gene="preT"
                     /locus_tag="KLF07_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21448..22683
                     /gene="preA"
                     /locus_tag="KLF07_000192"
     CDS             21448..22683
                     /gene="preA"
                     /locus_tag="KLF07_000192"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBD9830327.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="KLF07_000193"
     CDS             complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="KLF07_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBD9830328.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="KLF07_000194"
     CDS             complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="KLF07_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBD9830329.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="KLF07_000195"
     CDS             complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="KLF07_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBD9830330.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26695..27735)
                     /gene="galS"
                     /locus_tag="KLF07_000196"
                     /pseudo
     CDS             complement(26695..27735)
                     /gene="galS"
                     /locus_tag="KLF07_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27877..29034)
                     /locus_tag="KLF07_000197"
     CDS             complement(27877..29034)
                     /locus_tag="KLF07_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBD9830331.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(29051..29719)
                     /gene="folE"
                     /locus_tag="KLF07_000198"
     CDS             complement(29051..29719)
                     /gene="folE"
                     /locus_tag="KLF07_000198"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBD9830332.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29977..30813
                     /gene="yeiG"
                     /locus_tag="KLF07_000199"
     CDS             29977..30813
                     /gene="yeiG"
                     /locus_tag="KLF07_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBD9830333.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="KLF07_000200"
     CDS             complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="KLF07_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBD9830334.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="KLF07_000201"
     CDS             complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="KLF07_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBD9830335.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34802..35683)
                     /locus_tag="KLF07_000202"
     CDS             complement(34802..35683)
                     /locus_tag="KLF07_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9830336.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35782..36831
                     /locus_tag="KLF07_000203"
     CDS             35782..36831
                     /locus_tag="KLF07_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBD9830337.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36905..37762
                     /gene="nfo"
                     /locus_tag="KLF07_000204"
     CDS             36905..37762
                     /gene="nfo"
                     /locus_tag="KLF07_000204"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBD9830338.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37766..38854
                     /locus_tag="KLF07_000205"
     CDS             37766..38854
                     /locus_tag="KLF07_000205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBD9830339.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38910..40160)
                     /locus_tag="KLF07_000206"
     CDS             complement(38910..40160)
                     /locus_tag="KLF07_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD9830340.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="KLF07_000207"
     CDS             complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="KLF07_000207"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBD9830341.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41331..42029
                     /gene="yeiL"
                     /locus_tag="KLF07_000208"
     CDS             41331..42029
                     /gene="yeiL"
                     /locus_tag="KLF07_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBD9830342.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42096..43346)
                     /locus_tag="KLF07_000209"
     CDS             complement(42096..43346)
                     /locus_tag="KLF07_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD9830343.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="KLF07_000210"
     CDS             complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="KLF07_000210"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBD9830344.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44366..45307)
                     /locus_tag="KLF07_000211"
     CDS             complement(44366..45307)
                     /locus_tag="KLF07_000211"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBD9830345.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="KLF07_000212"
     CDS             complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="KLF07_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBD9830346.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="KLF07_000213"
     CDS             complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="KLF07_000213"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBD9830347.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="KLF07_000214"
     CDS             complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="KLF07_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBD9830348.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49909..51090
                     /gene="setB"
                     /locus_tag="KLF07_000215"
     CDS             49909..51090
                     /gene="setB"
                     /locus_tag="KLF07_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBD9830349.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51087..51341)
                     /locus_tag="KLF07_000216"
     CDS             complement(51087..51341)
                     /locus_tag="KLF07_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBD9830350.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51496..52068
                     /gene="yeiP"
                     /locus_tag="KLF07_000217"
     CDS             51496..52068
                     /gene="yeiP"
                     /locus_tag="KLF07_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBD9830351.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52291..53757
                     /locus_tag="KLF07_000218"
     CDS             52291..53757
                     /locus_tag="KLF07_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD9830352.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53875..54861
                     /gene="yeiR"
                     /locus_tag="KLF07_000219"
     CDS             53875..54861
                     /gene="yeiR"
                     /locus_tag="KLF07_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBD9830353.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54900..55613
                     /gene="lpxT"
                     /locus_tag="KLF07_000220"
     CDS             54900..55613
                     /gene="lpxT"
                     /locus_tag="KLF07_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBD9830354.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            56025..56591
                     /gene="mepS"
                     /locus_tag="KLF07_000221"
     CDS             56025..56591
                     /gene="mepS"
                     /locus_tag="KLF07_000221"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBD9830355.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56772..58328
                     /locus_tag="KLF07_000222"
     CDS             56772..58328
                     /locus_tag="KLF07_000222"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBD9830356.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58410..60224
                     /locus_tag="KLF07_000223"
     CDS             58410..60224
                     /locus_tag="KLF07_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD9830357.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60225..61319
                     /locus_tag="KLF07_000224"
     CDS             60225..61319
                     /locus_tag="KLF07_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBD9830358.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61319..62344
                     /locus_tag="KLF07_000225"
     CDS             61319..62344
                     /locus_tag="KLF07_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9830359.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62346..63935
                     /gene="yejF"
                     /locus_tag="KLF07_000226"
     CDS             62346..63935
                     /gene="yejF"
                     /locus_tag="KLF07_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBD9830360.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63939..64283)
                     /locus_tag="KLF07_000227"
     CDS             complement(63939..64283)
                     /locus_tag="KLF07_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830361.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64616..65736)
                     /locus_tag="KLF07_000228"
                     /pseudo
     CDS             complement(64616..65736)
                     /locus_tag="KLF07_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="KLF07_000229"
     CDS             complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="KLF07_000229"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBD9830362.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66609..68369
                     /locus_tag="KLF07_000230"
     CDS             66609..68369
                     /locus_tag="KLF07_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBD9830363.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68494..68778
                     /gene="rplY"
                     /locus_tag="KLF07_000231"
     CDS             68494..68778
                     /gene="rplY"
                     /locus_tag="KLF07_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBD9830364.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="KLF07_000232"
     CDS             complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="KLF07_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBD9830365.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70106..70333
                     /locus_tag="KLF07_000233"
     CDS             70106..70333
                     /locus_tag="KLF07_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBD9830366.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70353..72113
                     /gene="pbgA"
                     /locus_tag="KLF07_000234"
     CDS             70353..72113
                     /gene="pbgA"
                     /locus_tag="KLF07_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBD9830367.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72188..72264
                     /locus_tag="KLF07_000235"
     tRNA            72188..72264
                     /locus_tag="KLF07_000235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72222..72224,aa:Pro,seq:ggg)
     gene            complement(72367..74367)
                     /gene="yejO"
                     /locus_tag="KLF07_000236"
                     /pseudo
     CDS             complement(72367..74367)
                     /gene="yejO"
                     /locus_tag="KLF07_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18268 a        19590 c        18256 g        18253 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgtaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aagcatcact ttaccagttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgctaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accaacaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcggt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    15361 gggggcggcc aatcgccgcc gccccctgca cccccgggct ctggcgaaca aaatcgccgc
    15421 tgcgcggtgc cctcggctta tcccttacgg ctaccgggtc gggcgcgagg taacatccct
    15481 gtaaaacgcg ccctcagccc acctccatgt gggctgcccc ggccttcagg gaacgcctcg
    15541 gcaattttga cgccaccaaa caaccgtgcg gcctattgat aaagagctaa cacattgtca
    15601 aaaaacatca ctatggtttt ttagagtttc tcgatatcaa ttgcctgaat agcccttgca
    15661 atatcagggg aattattcaa cacccgaaca tgctgaaata attccgttgc ttcatcgtat
    15721 tctttacgca aataactcaa ccactgttta atccgcgcaa cgtgatataa cccggtatcg
    15781 ccctgctttt ccagacgggt atatttttgc agcaaagcaa ccacctccgg ccacggcatt
    15841 cgcggttcgt tatattttac tacccggctc aggttgggaa tattgagcgc cccgcgacca
    15901 atcatcactg cgtcgcagcc gctgatcgca atgcattgtt gcgcgctctg ccagtcccag
    15961 atttcgccgt tggcaatcac cggaatattc agccgctggc gaatctcgcc aatcgcctgc
    16021 cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc gtccatgcac caccagctcc
    16081 gtagcaccag cctgttgaac cgcatcggcg atttcaaatt tcttctcacc gctgtcccag
    16141 cccagacgca ctttcacgct gacgggcaaa tgcgccggta cagcttcacg catcgctttt
    16201 gcaccctggt agatgagttc aggatctttg agtaacgtcg ccccgccgcc gctgccgtta
    16261 accgttttcg acgggcagcc gcaattgaga tccacgcccc aggaacctaa ttccaccgca
    16321 cgggcggcgt tctctgccag ccattgtggg aactgaccta acaactgaac gcgcaccagc
    16381 gtaccggatg gtgtccggct ggcgttttgt agctcagggc aaatgcgatg aaagactttt
    16441 accggcagca gttgatccac cacgcggaca aactcggtga tgcacagatc gtagtcgtta
    16501 acttcggtca gcaattctcg caccagagag tcaagcacac cctccatcgg agccagtaac
    16561 acacgcatat cttcacccgg aaaaaaatga ggcgctatgt tagcgcctat ggtcaacgga
    16621 ttaaaggtct gcaaagaggg attgctgcaa actgtaatcc ctctctatgc ttgatgtatg
    16681 cctgagttcg ttgcgcattg cgtaatgccg gggagcgaaa aaataagcgc tgatttctta
    16741 atgtgatcgg tagcacgttt tacgaattaa ttgtatgatg aatccatctc atctggggtg
    16801 ttgattatga gtaagacact gaacattatc tggcaatatt tacgcgcttt cgtcctgatt
    16861 tatgcctgcc tgtatgcagg cattttcatt gcttccctgc taccggtaac cattccgggc
    16921 agcatcatcg ggatgctgat cctgtttgtc ctgctggcct tgcaaattct tccggcaaaa
    16981 tgggtcaatc cggggtgcta cgtactgatt cgctatatgg cgctattgtt tgtgccgatt
    17041 ggcgtaggcg tcatgcaata ttttgatttg ctccgcgcac agtttggccc ggtagtggtt
    17101 tcctgtgcag tcagtacgct ggtggttttt ctggtggtga gctggagttc acaactggta
    17161 cacggtgaac gtaaagtcgt aggtcagaaa ggatcagaag aatgatggcg aatatctggt
    17221 ggtcattacc gctgacgttg attgtctttt ttgccgcccg caaactggcg gcacggtata
    17281 agtttcccct gcttaacccg ttgctggtag caatggtggt gatcattcct tttttaatgc
    17341 tgaccggcat ctcttacgac agctacttta aaggtagcga agtgctaaac gatctgctgc
    17401 aaccggcggt cgtcgcgctg gcctatcctt tatatgagca gctacaccag atccgcgcgc
    17461 gctggaaatc gatcatcacc atctgtttta tcggcagcgt ggttgcgatg gtgacgggga
    17521 cttccgtggc attgttgatg ggcgcttcac cggaaatcgc cgcgtcaatc ctgccgaaat
    17581 cagtcaccac gcctattgca atggcggttg gcggcagtat tggcggtatt ccggcaatca
    17641 gcgcagtttg cgtgattttc gtcggcatcc tcggcgcggt atttggccat acattgctta
    17701 atgcgatgcg tattcgtacc aaagctgcgc gcggtctggc aatggggaca gcctcgcacg
    17761 ccctcggtac ggcgcgctgc gccgagctgg attatcagga aggtgcattt agttcgctgg
    17821 cgctggtgtt atgcgggata attacttcgc tgatcgcacc gttccttttc ccgattattc
    17881 tggcagtaat gggctaaaat ttgcgatgcg tcgcgcattt ttgatgtaag tttcacgcgt
    17941 tgcataatta atgagattca gatcacatat aaagccataa cgggttcgta aactgttatc
    18001 ccattacatg attatgaggc aacgccatgc atccacgttt tcaaaccgct tttgcccaac
    18061 ttgcggataa cttgcaatct gcactggaac ctattctggc agacaagtac ttccccgctt
    18121 tgttgaccgg ggagcaagtc tcatcgctga agagcgcaac ggggctggac gaagacgcgc
    18181 tggcattcgc actacttcca ctggcggcgg cctgtgcgcg tacgccattg tcgaatttta
    18241 atgttggcgc aattgcgcgc ggtgtgagcg gaacctggta tttcggtgcc aatatggaat
    18301 ttattggtgc aacaatgcag caaaccgttc atgccgaaca aagcgcgatc agccacgcct
    18361 ggttgagtgg tgaaaaagcg cttgcagcca tcaccgttaa ctatacgcct tgtggtcact
    18421 gccgtcagtt tatgaatgaa ctgaacagcg gtctggatct gcgtattcat ctgccgggcc
    18481 gcgaggcaca cgcgctgcgt gactatctgc cggatgcctt tgggccgaaa gatctggaga
    18541 ttaaaacgct gctgatggac gaacaggatc acggctatgc gctgacgggt gatgcgcttt
    18601 ctcaggcagc gattgcggcg gcaaaccgtt cgcacatgcc ttacagtaag tcgccaagcg
    18661 gtgtcgcgct ggaatgtaaa gacggtcgta ttttcagcgg cagctacgct gaaaacgccg
    18721 cattcaaccc gactctgcca ccgttgcagg gggcgttaat tctgttgaat ctcaagggtt
    18781 atgattaccc ggatatccag cgcgcggttc tggcagaaaa agccgatgcg ccgttgattc
    18841 agtgggatgc cacctccgca acgctgaaag ctctcggctg tcacagtatc gaccgcgtgc
    18901 ttctcgctta agcctggtgc cggatgcggc gtgaacgcct tgtccggctt gccagccctc
    18961 tcttggtgtc gaaattcccg gcaaacagtt tgccgtttct tgcgcaaaac cagcgggtaa
    19021 agtagcctga tggaaatttt ccttagatcg agtctcctgc atgttaaagc gcgtgttcct
    19081 cagcctgtta gtcctgatcg gcttgctgct gttgactgtg ctcggcctcg atcgctggat
    19141 gagctggaaa accgcgcctt atatctacga cgaattgcag gatctcccct accgccaggt
    19201 cggcgtggtg ctcggcacag caaaatatta tcgtactggc gtaattaatc agtattatcg
    19261 ctaccgcatt caaggagcga ttaatgccta taacagcggt aaggtaaatt atctattact
    19321 gagcggcgat aacgcattgc aaagttataa tgagccgatg accatgcgca aagatttaat
    19381 cgctgctggt gtcgacccat cagatattgt tctcgattac gcaggctttc gtacgctgga
    19441 ttccatcgtg cgtacacgca aagtgttcga tactaatgat ttcattatta tcacccaacg
    19501 tttccactgt gagcgggcat tatttattgc gctgcatatg gggattcagg ctcagtgtta
    19561 tgccgtaccg tcaccgaaag atatgctgtc agtacgtatt cgcgaatttg ccgcccgttt
    19621 cggtgcgctg gctgaccttt atatttttag acgtgaaccg cgttttttag ggccgctggt
    19681 ccctattccg gctatgcacc aggtgccgga agatgcgcag gggtatcccg ccgtcacacc
    19741 cgaacagtta cttgaattac aaaagaaaca aggaaagtag ttatggatgt acagcagttt
    19801 tttgtcgttg ccgttttttt ccttatcccg atattttgtt tccgcgaagc atggaaaggc
    19861 tggcgcgcag gcgcgattga taaacgggtt aaaaatgcac cggaaccggt gtatgtctgg
    19921 cgagcaaaaa atcccggact ctttttcgct tatatggtgg catatatcgg cttcggaatt
    19981 ttatctatcg gcatgattgt ttatcttatt ttctatcgtt aatattttct cttttctgtt
    20041 aattattgag aataattatt acttcactca ataagctgcg gatataacat tctgaaccgc
    20101 agctatttgt gaatcctttc acagtttaaa tttccccaca cgcttagcct taatatcagc
    20161 acgcaatatt tatttagcgt tcgccttaat tactcatagc attaaggaag atcacatgcc
    20221 gcaacaaaat tatctggatg aactcactcc ggcttttacg cctttactgg cgattaaaga
    20281 agcctctcgc tgtttattat gtcatgacgc tccctgtagt caggcttgcc cggcacagac
    20341 cgatccgggg aaatttattc gctcaatcta ctttcgtaat tttaaaggcg ctgccgagac
    20401 aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga gtgtgcccga cggagaaatt
    20461 atgccaaagc ggttgtaccc gtgccggtgt tgatgctccc attgatatcg gccgcttaca
    20521 gcgttttgtt actgattttg aacaacaaac cgggatggaa atttatcagc ccggtactaa
    20581 aacgctcggc aaagtcgcga ttattggcgc aggtcctgcc ggattgcagg ccagtgtgac
    20641 actgacaaac cagggttatg acgtcacgat ctatgagaaa gaagcacaac ccggtggctg
    20701 attgcgtaac ggcattccgc aattccggtt accgcaatca gtactggatg cagagatcgc
    20761 ccgtattgaa aaaatgggcg tgaccattaa gtgcaacaac gaagtcggta acacactcac
    20821 ccttgagcag ctgaaagcag aaaaccgcgc ggtactggtc accgtggggt tatcaagcgg
    20881 ttccgggcta ccgctgtttg agcatagtga cgttgagatt gccgtcgact tcttgcaacg
    20941 tgcacgacag gcgcaaggcg atatcagcat tccacaaagc gcattaatta tcggcggcgg
    21001 tgatgtcgcg atggacgtag ccagcacgct gaaagttctc ggctgtcagg cggtaacttg
    21061 cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa aaagagttta ccagcgcccg
    21121 ggagctgggc gtttcgatca tcgatggatt cacgccagta gccgtggaag gcaataaagt
    21181 cacgtttaag catgtacggt tatcgggcga actgacgatg gcggcagata aaattattct
    21241 cgccgtcggt cagcatgcca gactagatgc ctttgcggag ttagagccgc agcgtaacac
    21301 catcaaaaca caaaattacc agacccgcga cccgcaagtc tttgctgctg gcgatattgt
    21361 tgagggagac aaaaccgtgg tctatgccgt gaaaaccggg aaagaagccg ccgaggcgat
    21421 tcatcactat ttagagggag cttgctcatg ttaacgaaag atctttcgat tactttttgc
    21481 ggcgtgaagt ttcccaaccc gttctgcctc tcttcttcgc cggtaggcaa ctgctatgag
    21541 atgtgtgcca aagcctacga cacaggttgg ggcggtgtgg tgtttaaaac gatcggcttt
    21601 tttatcgcca acgaagtctc gccgcgtttt gatcatctgg tgaaagaaga taccggtttt
    21661 atcggcttca aaaatatgga gcagattgcc gagcatccgc tggaggagaa tctggccgcc
    21721 ctgcgtcggc tgaaagaaga ttacccggac aaagtattga tcgcttcgat catgggggaa
    21781 aatgagcagc aatgggagga gctggcgcgc ctggtgcaag aagctggcgc ggatatgatc
    21841 gagtgtaact tctcctgtcc gcaaatgact tctcatgcga tgggtagcga tgtcgggcaa
    21901 agcccggagc tggtagaaaa atattgtcgg gcagtgaaac ggggttccac gctgccaatg
    21961 ctggcgaaga tgacgccgaa tatcggtgat atgtgcgaag tggcgctggc ggcgaagcgc
    22021 ggcggcgcag atggcattgc ggcgattaac accgttaaat ccatcaccaa tatcgatctt
    22081 aatcagaaaa tcggtatgcc gatcgttaac ggaaaatcaa gtatttccgg atattccggt
    22141 aaagcggtaa aaccgatcgc cctgcgcttc attcagcaaa tgcgcaccca tccagaactg
    22201 cgcgatttcc caatcagcgg tatcggcggc attgaaacct gggaggatgc ggcggagttt
    22261 ttattgctcg gcgcagcaac gttacaggtc accaccggca ttatgcagta cggctatcgc
    22321 atcgtagaag atatggcgag cgggttatcg cattatctcg ccgatcaggg atttgattcg
    22381 ttgcaggaga tggtaggtct ggcgaataac aatattgtcc cggcggaaga tttagaccgc
    22441 agttatattg tctatccccg tatcaatctt gacaaatgtg ttggctgtgg acgctgttat
    22501 atttcctgtt acgacggcgg tcaccaggcg atggaatgga gcgagaaaac ccgcacaccg
    22561 cattgtaata ccgagaaatg tgtgggttgt ctgctttgtg gtcacgtctg cccggtgggt
    22621 tgtattgagc tcggggaagt gaagtttaag aaaggcgaga aagaacaccc ggtaacgttg
    22681 tagaacccgc gtcacatccg gcatttggtt cctgttgccg gatgcggcgt gaacgcctta
    22741 tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg aatgcggacc
    22801 acattcacat catttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat
    22861 aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata
    22921 gttgatgacg gtaaaaataa tcaccccggt caccacgcca atcaccgtcc ccacaccgcc
    22981 gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc
    23041 gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa
    23101 cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt
    23161 tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca
    23221 caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa
    23281 actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat
    23341 tggcgacgcc ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt
    23401 gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa
    23461 accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc
    23521 catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac
    23581 tgccgccagc cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc
    23641 tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt
    23701 caacagactt aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac
    23761 gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat
    23821 gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt
    23881 tgtttttgta tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt
    23941 tatccctaac aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc
    24001 cagttcggca attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg
    24061 ttcatcgagc attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg
    24121 ctgattacca ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat
    24181 cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt
    24241 atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata
    24301 aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta tggcttcgtt
    24361 ggcattatgg ttattgatct gtttgccgtg caacgtaatg gtgccagccg atttctcgcg
    24421 aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc
    24481 gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga
    24541 cgttaggtta cgcacctcga ggatgacttc gcccggcttg ttttctttgt caggaaaacg
    24601 ctgattaaga gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag
    24661 cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa
    24721 gatttcttcc attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg
    24781 aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat
    24841 aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga
    24901 aacggataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat
    24961 tgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg
    25021 ccccagccac atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac
    25081 catcgaaata ccattttcca gtgcctcttt ggcggaatgg aaatcgatct ctttaccctg
    25141 gaataaaatg gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga
    25201 ttttcctgca ccgttttccc ccattaatgc atggatagaa tgtggccgga cttttaaatt
    25261 aacgttatca agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa
    25321 gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac
    25381 aataagggcg cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag
    25441 gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt
    25501 ggtgccatca gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt
    25561 cgcctggttg ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc
    25621 cagcgcttct ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt
    25681 caatgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt
    25741 gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtgtccca
    25801 cattgcggta tctaactgta actgttcagt tttgatgcct ttatcgttca attctttaat
    25861 cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcagtacgaa
    25921 ctgaatctga ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat
    25981 caaatcgccc tgaataatgc cggactcttt ggagtcagtg ccaacgtagt aggctttgtc
    26041 gtagctatcc agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg
    26101 cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag
    26161 tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt
    26221 ctgagaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc
    26281 cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt
    26341 atcagcagcg tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa
    26401 caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg
    26461 ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac ttttttaaat
    26521 tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat
    26581 tacctcggtg aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa
    26641 tcatagctat cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat
    26701 cgcctgattt gttgagttag tgatcgccgc cgcattctgg cgcgttgcga cagaatggcg
    26761 acgcactaac gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc
    26821 ctgcaaggcc agttcggtgg ctaatttagc cattgaagca atgggataac gcacggtcgt
    26881 taattgcggg tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg
    26941 taacggaatc gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc
    27001 gttataagca aatatagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc
    27061 ctcaccgccc ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc
    27121 ctgctctttc aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg
    27181 actggaagaa agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat
    27241 tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg
    27301 gttgattaac accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa
    27361 tgcttttgag tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg
    27421 acgctctttt tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg
    27481 agcgactaga tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac
    27541 caccacgcca atggtgtcgc taacctgagt tgccagcgcc tgggcattgg cgtttggccg
    27601 ataatccagc tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt
    27661 gctgttattg agcacccggg aaaccgttgc cacagagacg ccagcctaac gcgctacatc
    27721 acgaatggtg atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc
    27781 ggctattttg tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg
    27841 ttacagccgt tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc
    27901 cgttccggca gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa
    27961 gtaacgcagc cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc
    28021 cagcagctcc aggcggtcaa aatgcataaa caaaccgagg tggtaaaaaa gcatggtaca
    28081 aatcagcgtt tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag
    28141 caccagctta aagcggctca attgcggcca gaagccataa aacagcgacg catagccaat
    28201 cgcctgaaac ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata
    28261 cgcccagtcc agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc
    28321 caccagcaca aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc
    28381 gctgcgcatc agcgccgcac caatgagcat catccctgcc agttgccagc catactgtgc
    28441 gcccagtgcc agtaaactgt tgccaacacc atcggcacga ttactgatcg cttcaacgcc
    28501 gccgtgaagc ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggtgcg
    28561 gctggtctgg ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata
    28621 aagcatgacg cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca
    28681 gcagattaag cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc
    28741 gtgaataaaa cccagcaaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg
    28801 gggcagcaac atttgcaggc ccgcaccaaa cagcagcgca aaaagcgtga ggaatttcac
    28861 ctggccgatg agatcgagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcgccgta
    28921 ccaggcggga ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag
    28981 gatccccaga atggcgacgc cgcgaacaaa atcgagcgtt acgttgcgct ccatggttcc
    29041 tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac
    29101 tggatttgaa caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc
    29161 cacgcgcttt cacgcagtaa tgcaccgcat caatggagac agccacgtta ttggtgccca
    29221 gcagcgtttg tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct
    29281 gggcaaagaa ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga
    29341 tataagccac cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca
    29401 gagtgatatc gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga
    29461 ttttcgggaa gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga
    29521 tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga
    29581 tttcggtcat atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg
    29641 gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt
    29701 ctttactgag tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg
    29761 ggggcaaagt tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa
    29821 tccagtattc acagcgataa ttattgtaat tgccgctgac tttcatcagc agatgttaaa
    29881 acatcgttat gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga
    29941 tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc
    30001 tgttttgaag gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg
    30061 acgttcagta tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg
    30121 ctttccggat taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg
    30181 gcggcagaac tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt
    30241 gccaacgacg atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa
    30301 ccgccgtggg cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg
    30361 gttcagtcgc aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt
    30421 cacggtgcgc tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt
    30481 gcgccaattg tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cacctattta
    30541 ggtgaggaca aaaatgcatg gctggaatgg gatagttgcg cgctgatgta tgccagtaac
    30601 gcgcaggatg cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac
    30661 cagttgcaac ctgcggtatt ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg
    30721 cgtattcagc cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac
    30781 ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt
    30841 ctcatcagaa gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt
    30901 agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt
    30961 ctttagtcac ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag
    31021 ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt
    31081 cttccagcgc cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg
    31141 atagcggttt gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg
    31201 acagtttcca ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag
    31261 ctttgttaac gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa
    31321 aaccaacaaa gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga
    31381 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    31441 cttcttcacc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    31501 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    31561 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    31621 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat
    31681 cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca
    31741 caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg
    31801 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    31861 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    31921 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    31981 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    32041 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    32101 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    32161 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    32221 tgccgtaagc tttcattccc agcacaccat caattaatgg tccactggta aagaactgac
    32281 cgttataggt atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac
    32341 ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg
    32401 aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg
    32461 ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttcacgcgtt
    32521 taccgtcgac gagaatcagg gtatagctgc tgtccagacc acgaatacta acgcccttac
    32581 ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat
    32641 tctgtaccgg ttttcgctgc agatcttcct gggtaatgac gctgatactg gcgggtgcat
    32701 ctttaaggtt ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcgtcatcga
    32761 ccgctaacac aggccataca caagaaatag cggacaaaca cagcccgacc cgtacgaaag
    32821 ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa tgggtatcgc
    32881 tcacatcttc tttacgtccc cttgcgtacg gcagcatcgc ggtggactta tttgtatgca
    32941 ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt gattgtagcg
    33001 taacgataat gcaaacgata acaattatca atccaaatgt taaattttat atccgcatga
    33061 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    33121 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    33181 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    33241 ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca
    33301 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    33361 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    33421 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    33481 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacaaaccg gcaatcaccg
    33541 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga caatttagcg aaaatgcgcg
    33601 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    33661 tacctgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    33721 gacccgcgtg ctggaacacc aaggtgaacg gactgacgct gatgtcttta acatcgttac
    33781 gcagcaggct cggatcggta tacggaataa tcaggctgat aatcaggatc gcgaacacat
    33841 agaacaacag gattcgccag aatacctgac gtaccgcgcg tggaatgttt ttcgccggat
    33901 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    33961 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    34021 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    34081 cgatgataaa gacaataact gtcgtgactt tgatcagtga aaaccagtat tccgcttcac
    34141 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    34201 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgtgct gcaaccaagt
    34261 caacggcaat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    34321 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    34381 ccaattcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    34441 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    34501 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    34561 agcccggcgc ttctgtagtt ttagtttcgg aacccataca aaaatgctat ccatcttaaa
    34621 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    34681 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    34741 gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcac
    34801 cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    34861 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggg agagggaccg
    34921 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    34981 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    35041 ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg
    35101 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac
    35161 gagccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    35221 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    35281 gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc aactgcggat
    35341 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    35401 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    35461 caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    35521 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    35581 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    35641 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    35701 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    35761 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    35821 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    35881 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    35941 gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg
    36001 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    36061 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc
    36121 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    36181 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    36241 tcggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    36301 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    36361 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    36421 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    36481 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    36541 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgtc
    36601 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    36661 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    36721 gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    36781 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    36841 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    36901 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    36961 tcgcgccgtc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    37021 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    37081 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    37141 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    37201 acaactgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    37261 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    37321 tgtaacagcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    37381 acatctcgcg gcgattatcg acggcgtgga agataaattc cgcgtcggcg tctgcattga
    37441 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgaaaaaac
    37501 gttcgctgat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga
    37561 tgcgaaaagt acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    37621 cggtcatgat gcgttccgct ggatcatgca agacgaccgt ttcgacggca ttccgctgat
    37681 ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    37741 aactgaaaaa gcggtggcct gatcgatgaa cgacagggaa aagcagattc ttaaaatttt
    37801 acggcgtaac ccgctgattc aacagaatga aatcgccgat attctgcaaa tcagccgctc
    37861 acgtgttgcc gctcatatta tggatttgat gcgcaagggt ttgattaaag gcaaaggata
    37921 tatccttacc gagcaggatt actgtgtggt tgtgggtgca atcaatatgg atattcgcgg
    37981 aatggcggat atccgttacc cgcaagcagc ctctcatcca ggttctgttc actgttccgc
    38041 aggcggcgtc ggacgtaata tcgcgcataa cctggctctg cttggtcggg atgtgcattt
    38101 aatatcagct attggcaacg atttttacgg cgagacatta ctggaagaaa cgcgccgcgc
    38161 gggcgtgaac gtttcaaact gcattcgtct gcatggacac agcactgcaa cttatctcgc
    38221 tatcgctaat aagcaggaag aaactatcct ggcaatcaat gacacacata ttctgcaaca
    38281 gctaacgcct caactgctca atacttcacg ggacttgatt cgccatgctg gtgtggtgct
    38341 ggctgattgt aatctcacac ctgaagccct ggagtgggtc tttaccattg ctgatgaaat
    38401 tccgatgttt gtcgataccg tttccgaatt taaagcgaac aaagtaaaaa actggtacag
    38461 ccggatccac actctgaaac caacacaaaa tgaactggaa attttgtggg ggcaaccgat
    38521 taaagacgac aacgatcgca tcagagctgt aaattcgctg catcaacaag gtgttaaacg
    38581 catttttgtt tatctgaaag atgaatctgt attttgcagc gataaggatg gagaacaatt
    38641 tttgctgacc gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat
    38701 ggcgggcctg gtatatagct ttcttgaagg atacagtttc cgcgacagcg cccgttttgc
    38761 ggtagcctgc gcggcaattt cgcgcgccag cggcagctta aacaacccta ccctgtctgc
    38821 cgataacgcg ctttcattag tgccaatggt gtaacaatgt tgccggattc gacgcttgac
    38881 cgcatcttat ccggctcata aattgcgcac tacgctaaac caataaagaa tcctgcaata
    38941 gtagcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagttgggcg
    39001 atttccggcg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa
    39061 ccaaagttag cgaaaccgca caacgcgaaa gaaataatcg ccacggtttt agcatccaga
    39121 gtgccacccg tttgcagata tggcgagaaa ttgagataag cgacaaattc attgatcgcc
    39181 agcttctgcc caatcaaact cccggcaaga tttgcatcac tccagtcaac ccccatcacc
    39241 cacgccaatg gggccaacag gtaacctaaa atcgactcca gcgaagcatg ttcaaaacca
    39301 aaccagccgc caacaccgcc gataataccg ttaatcaacg caattattgc aacaaatgcc
    39361 ataacaactg tcgctacacc ggcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg
    39421 gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa agaaacctgc
    39481 gaagattccg tagccgggct taacaggcgg gcaaacaaga tcccgccagg gatcgccatt
    39541 aacgatgccg ccagcaaata ttccacaggt acgcccagtg cggcgtaacc aatcattgtc
    39601 gaaccagcaa tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga
    39661 cgatcgataa agggtttgac gatcgccgga atttcgtttt gcccgaggaa aatggtggtg
    39721 accgcgacga atgactcgat cttgctgata tttaaagctt tctggaagat accgccgaga
    39781 atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag tgcagtgacg
    39841 aagataattg ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaataaggta
    39901 tccattttcg gtccgaccag agaaccgaag ataaatgcgc tacctgcgtc gctgtacgcc
    39961 atcactttgt ggacgccaaa agcgactttt tctgcgaccc aacgccctgg cggtaaccaa
    40021 agcataatcc cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg
    40081 atcttcttct tgtttactga cagtaaaaac gcaattgcca gcagtaccac cattcccaga
    40141 acacttctca tgacatccat agtgattatc tcttcatgtc attttgggcg acggctttct
    40201 gcccatcaga aatagccggg cctgcatctt gcgatactgt cccggcttgt tatcaacact
    40261 taatgggttt ttatgtagcc gcgtacgcac tcttcgacta atccccagaa ccagtcggta
    40321 tcaatagtga tgccgacttt agtattggcg ggcttgccaa gaacgcccag ctcgtcgcag
    40381 acggtacgcc catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgggtt
    40441 ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca
    40501 gccaggccgt agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacatc
    40561 tctccggcag ggccgcctgc cctttccatt cgtgcaatta cgtccggggt gcaaacggtc
    40621 tggttggtaa gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgt
    40681 gcggcttccg ggtcggcaaa gatattgaac tcggcagatg gggtgaagtt gcctgtaccg
    40741 taagcgccgc ccattagcac aatttcacgg attttgggca ggattgcggg ttgcatacgc
    40801 attgccaccg cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt
    40861 gccataaggg tatcaatgat gtatttcacc gcgtgggtac tttctgcctg gcgggtcagc
    40921 ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt
    40981 tgacgcataa tgggttgcgg catccccgca taaaccggaa cattaatctc cagtttctgg
    41041 caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc aacaatggtg
    41101 atacctaata aatcaatcgc cggatgtttc gccgccatca ttatagcaat agcatcatca
    41161 tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc
    41221 cagttgcgtt attttttcta cagcaacgaa atttcgatcg ggccgatgat tgaatcttaa
    41281 caacagcgta cgaatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa
    41341 tccacaataa tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt
    41401 taacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc
    41461 aggaagggca acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg
    41521 ccacgctagc taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg
    41581 gcgagattga gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt
    41641 gttggtgcct tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat
    41701 ttttacgaaa actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa
    41761 ctcagaatca atcatttccg ttagtgaatc gcctggcagc atttatatta ctctcgcagg
    41821 aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc
    41881 gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt aaaagcaaga
    41941 aagggtatct aattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg
    42001 agaataaatt ctccgggatg atgcagtaaa aattattggc aatagcacga ttgccggatg
    42061 caacgcttaa taaggcgtac atccggcaat gaaactcaag ctaaaccaat aaagaacccg
    42121 gcaatggtcg cgctcatcag gttggaaagc gtcgccgccg ccagcgcccg taaaccaagc
    42181 tgggcgattt ccggcgcgcg ttttggcgaa atagccgaaa acgctccaac gacaacaccg
    42241 atagaaccaa agtttgcaaa cccacagagg gcgaacgaga taatggcaat ggtcttgaca
    42301 tccagcgtgc cgctcgtttg cagatagggc gagaaattga gataagcaac aaactcatta
    42361 atcgccagtt tctgcccaat caaacttccg gcaagatttg catcactcca gtccaccccc
    42421 ataatccatg ccaacggggc cagcacatag ccgaaaatgc tttccagaga gacattggca
    42481 aaaccaaacc agccgccaac tccaccaata atgccgttga tcagcgcaat gatcgctaca
    42541 aatgccatta ccaccgtcgc cacaccggcg gcgattttca gcccggtcat tgcaccgttc
    42601 gccgcagctt caataatgct ttttggcggt gtttcgctaa acgacagatt ttcaaacgta
    42661 acttgcgaag gttccgtcgc cggactaaga atacgtgcaa acaggatacc gccaggaata
    42721 gccatcagcg aagcggctaa caaatagtca atcggcaccc ccattccggc atagccaatc
    42781 atcatcgaac ccgcaataga cgccatgccg ctacaaatag ccgtaaatag ctcgttgcga
    42841 ttcatgcgat caataaaagg tttaacgatc gccgggatct cattttgccc gaggaaaata
    42901 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg aaaaatactg
    42961 ccaagaatac gaatcagcag ccccattacg ccaatgtagt acagcaagct gataagcgcg
    43021 gtaacgaaaa taatcgccgg gagtacgcga aaggcgaaga taaagcccgc accgtcaaac
    43081 agaacatcca ttttgggacc gaccagcgaa ccaaaaataa aggcacttcc tgcgtcactg
    43141 taggacatca ctttatggac gcctaatgcc gcctgctcta ctgcccattt tcccggggga
    43201 aaatagagca tgatgccacc aatggcaatt tgcagcagca gcgcagcgcc aaccgtgcgc
    43261 aagctgatgc tctttttatt taccgacaac acaaaggcaa ttgccagcaa taccaccatc
    43321 cccgcaacac ttctcattat atccataatg attttccctt catgccagta aacccggcgt
    43381 cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc cgccagaaat
    43441 taacccacga gacgctgata ttctttggca atttcgctcg ccagaatggc gttattaaag
    43501 accagttgaa tattggattt caggctatca ccgccggtta gctccgcaac acgcgccagc
    43561 aggaacggtg tactctcttt cccaataaca ccctgttctt cggcttcagc aacggcttga
    43621 tcaattgctg cattgatggt ttcttctggc atcgcaaact gttccgggat cgggttcgct
    43681 accaccaggc cgccgttcaa accggtttgc cacttcactg ccatcgcgcg agcaatttcg
    43741 cgggcactgt caagacgaat gctgacttca aatgggctgg ttcggcagaa aaacgcaggc
    43801 agcgcttttg tctgataacc aattaacggt acaccgaagg tttctaaata ttctgttgtt
    43861 aatcctaaat cgagaataga ttttgccccc gcacaaacaa cagtgacatt ggtatttgcc
    43921 agttcctgta aatcggcaga aatatcgaag gtatgttccg ctccgcgatg cacaccacca
    43981 atacctccgg ttgcaaaaac tttaatccca gccagtgctg caataatcat ggttgatgca
    44041 acggtagtcg cgccattttt ccctgcagca acgacaaaag gtaaatcacg acgactaact
    44101 ttcgtcacgt tatgtccttc acggcccagc aattcgattt cttctttact taagcccact
    44161 ttcatcacac cgccaataat ggcgatagtg gctggtactg cgccctgttt acgaattgtt
    44221 tcttctactt caattgcggt ttgggcattt tgtgggaatg gcattccgtg agaaattatg
    44281 gtcgattcca gcgcaacaac cggtttttta ttttttaaag catcctgtac ttcagcggat
    44341 atttgtaata attcagggga cagagttaat tcagacattc tgtgttctcc actaacgatc
    44401 taacattggc aattgataat tcagggttgt tggtatattc acaggcaagt gccattgacg
    44461 aacatccctg cgcaaatcga acagaatcga taaacggcat accgtctacc caacaggagg
    44521 ccagccccgc catcatggcg tcgcctgccc cagtaacatt gatgacattc gttttaatag
    44581 gggccgacca cccactttca ccgttgatat cactgtagta aacgccatcc cctcccatgc
    44641 tcagcaccaa gcggttcagg ccgtgttgat gaaaccaggc ggcgaccttt gcgacatctt
    44701 cacgtccgga aagtgcaatt ccgctcaacg tttcggcttc aagacggtta ggttttaacg
    44761 tatggatttt gctcagatga tcgcggattt tgacgcattt ccatgccgat accggatcaa
    44821 taaatacagg cgtttccccg ctattttcca gaacccagac cagcacctct tcactaaggt
    44881 tgcaatccgc gacaataacc tttgcaccgc gaataaactc ctgatgctgg gcaagaaatt
    44941 ctgcagaaat acagtcacta atactcatat catttatcgc gacaagcatt tctcctgtat
    45001 tatcgagcag tgataaatag ctggatgtat tctcacccgg aacaattaag catttatcga
    45061 cataaacacc ggattgatta gtttgcgcca gtaatgattg cccataaaaa tcaccaccta
    45121 ctgcacttaa aagccaggca tttttcccca gtaacgccag attatgagcg atattccttc
    45181 ccacgccacc tggggtaaat ttaatttttc cgggattcga atccgcataa ttcaatggat
    45241 gatgtgaata cccagccaca tcaatattcg ccgaaccaat aattactatg tagtcctttt
    45301 cgcgcatagc acgccccctg gcaataaatt gccaacgaag aaattgggta aaatagccat
    45361 tacagtcaat aagactatta gagcacgtgt tcagatttaa acacatgttc ataataagca
    45421 tatttaacgt aatgtaaatc gatcagaaaa aacgaaatcg gccagatatg aaggcggtca
    45481 caaagtttgt ctatttatgc agcaacaaaa gagaaagttg tgatgtgtaa cacacaatat
    45541 ggcaggcaag aaaaagggca gagatgtttc tgccctccag attgttgaca acgcgcgctt
    45601 tgttcatcct ggatgtggcg taaacgcctt atccgaccta caaaagcgca aaaaattcaa
    45661 tatattgcag ggttcatgta ggtctgataa gcatagcgca tcaggcaatt ttgcgtttgt
    45721 catcatcggg gcagagatgt ctcatccccg ataatgaaga taaattatgc gtttttttct
    45781 actatttgtg tttccgggcg tttcaggaag gcataggcca aacccgccac cagcgtaccg
    45841 gcaataattg ctaccaggta acccaatacc ggcgtaatag cgccagggat cagcagaaca
    45901 aacagaccac cgtgtggtgc catcagtttc gcaccaatcg ccattgagat tgcgccagtc
    45961 agcgccccac caacgataca gcacggtagc acacgcatcg gatcacgagc agcaaacgga
    46021 attgcacctt ccgaaataaa gcacagacca agtaccagag cggctttgcc accttcctgc
    46081 tgcgctttgt cgaatttgcg acgcgccacc attgttgcca gacccattgc cagcggtggc
    46141 accatacctg ccgccataat cgccgccatc gggccatagg tttgagtact cagcagaccc
    46201 acaccgaatg cgtacgctgc tttgtttacc ggaccgccca tgtcagtaca catcatgcca
    46261 ccgaggatcg cccccagcag aaccgcattc gcagtcccca tggtctgcag ccagtgagtc
    46321 aacccatcga gaatgccagc aaccggttta ccgattaggt agatcatcgc cagaccgacc
    46381 accagactgg aaattagcgg aatgatcagg atcggtttca gcgcctccat actctgtggc
    46441 agtttcaatt gcgtactgat taacttcgca atgtaaccag ccaggaagcc agcaattata
    46501 ccgccaataa agccagaacc ggtgctgacc gccagcatac cgccaatcag acccggtgtg
    46561 aggcccggac gatcggcaat ggagaaggca atataacctg ccagtaccgg caccatcagc
    46621 gcaaaggctg aaccaccacc aatctgcatc agcgccgctg ccaacgtacc cggctcttta
    46681 aacgcttcga taccaaaagc aaaagaaagc gcgatacaca gaccacctgc aacaaccatc
    46741 ggcagcatgt aagagacgcc cgtcagcaag tgacggtaag cgcctgcact ctctttctta
    46801 ccttcagtgg tcgctgtttg agctttgccc gctggttcat acggcgttgc ttcagcaacc
    46861 gctttatcca gttcctgcgc agttttcttc agcgccagac cggtagaggt gcgatacatc
    46921 ggtttaccag caaatttcgc cagatccact tcgatatctg ccgccacaat caccagatcc
    46981 gctgctgcga cttcttcagg ggtgattgca ttgcccgcgc caacagaacc acgggtttca
    47041 actttcaccc accagccacg ttttttcgct tcggtttcaa tggcttcagc cgccataaag
    47101 gtgtgtgcta cgccagtcgg gcaagcagtc accgcaacta cgcgtttcgg accgctggca
    47161 gcaaccggtg ctgtcgcagc gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca
    47221 ctcaggaaca gctcagggtg cgcaactgcc cgggaaatat cacccagcca gacattttta
    47281 ccgttcagcg cgctgtcatt cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg
    47341 ttcggattgt cgatgatttc cagttttgct tttcgcgccg ccgcgcccag cagggtcttc
    47401 gccatatagg cgcgtgcctg accgagatta gcgtcaataa tcagcagcgt tttcattatg
    47461 cctctcctgc tgtcagttaa aaggttgtaa gtcgacgcgc gccatcattg cggccaactg
    47521 cggacgatcg gtaataccca cattgctttg acttaccgcc agggctgcaa cagctgtcgc
    47581 cagacgcagt gtgtgttcac tggattcacg catcagcaag ccataaatca ggccaccaac
    47641 catagaatcc cctgcgccaa cggtgcttac gacatcgact gacggtggtt tggcgatcca
    47701 ttcgccggag gcattaaccc aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc
    47761 aataccttgt tcacgtagcg catgtgcagc ttcaatcaca tctttcattt caggcagttt
    47821 acggcctgcc cagatttcca actcgcggcg gttaggtttc accagccacg gtgccgcttt
    47881 caaacctgct actaacgctt cacggctact atcaaagata atgcaaggac actgactacg
    47941 caggcgagtc atccagtcgg tgaacgcttc cgggctgacg cctgacggta agcttccgct
    48001 gacacagacc atatcgaact gaccgagcca gctcagagaa tcagtcacaa agcgttccca
    48061 gtcggcgagg gtgacttcaa aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc
    48121 cgtcagctta acgttaattc gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc
    48181 cagctcgctg aacagttgct gaaaaccatc ctgattgtct ttacccagga agccgccaac
    48241 ggtgacatca attcccaggt cttttaatac tttggccacg ttgatgcctt tacccgccgc
    48301 atgcagaccg gtggttttca ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc
    48361 aacaaggtca taagccggat taagggtgat cgtagcaaca cgtctgctca ttatgcgccc
    48421 tccccaagac cagcagcgat agcgtcgcca atggctttca gcgcctgttc agcatctgca
    48481 ccctgggcgg taaagcgtag gcgatgacct ttcttaacgc caagtgccac aactttcatc
    48541 agactacgtc cgtttgccgg tttgccggtg ccatcaaggt ttgtcacggt aatatcactg
    48601 ttaaattgtt taatggtatt gaccagcatg gtacctggac gagcatgcag gccgtgttca
    48661 ttgcgcacca caaactccgc gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc
    48721 agcgccagca acgttgccgc atccgctttc agcaagcggt cagctttatt gtcgagcaat
    48781 aaatcagcga gacgcttaag aaccgcgatg ggctgatcgt cattcatcgc cacactcacc
    48841 agcatggctg ccgtttcgcc gtccacatca aaagcatttg ccgcacggct taccgcaatc
    48901 gcgctacgca gattgccttc ggcgctatcg ctcagccaga taccctgtcc gagattcagc
    48961 ggttgttcat tgatggcttt ggtgacgaaa gtggcgtcaa ctgcccccac ctctttcaga
    49021 cgcgcagcgt tcagcgcctg aagagtcagc agatcgctgg cgacgatatc cagcgtcagc
    49081 atttcgttgt cgagcttcag ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt
    49141 tcttctgctg ttgttgctga cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg
    49201 gtcagctggc gtagcaggcc cagatgttca tccgagctgg cagcaatacc gattgccacg
    49261 tacgctacct gaccgtcacc ccaggtgacg ccttccggga actgaaatac ctgaacgccg
    49321 gttttcagca cctgatcgcg ggtgtcggta gtgccgtgtg gaatagcaat accattgccg
    49381 aggaacgttg aggtttgctg ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca
    49441 ttaccggcct gcaccagcgc cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc
    49501 ttttcgcccg gatggatgtc ctgtacggat aactggaaca tagttctcct ctcttgctga
    49561 attgaaacga ttcagcctct atgagaaaaa aagcgccaac ctggcttagg gttaaagaca
    49621 agatcgcgct gaaacgtttc aagaaagcat aatacttctg tttcagcacg caaggaaagc
    49681 cgtaaagtta tgagcaaaag tttgatgtgc tgcacaattt tttgtcattt ttcctaattg
    49741 ctgatgggaa aatccgttat cagcaatttc atttcagcag cgtcagcagc caatcagcaa
    49801 cagtgaaatg gcattttgat tattcgtggc gcttttgatt tcgctgtctg tttattttct
    49861 gacaagcagc gtaaactccg cgtcttcctc ttccagtgat cgacctgcat gcataactcc
    49921 cccgcagtct ccagcgcgaa atcgtttgac ctgacctcga cggcgttttt aatcgttgcc
    49981 tttctcaccg gtattgcggg tgctctgcaa accccgacac tcagtatttt ccttaccgat
    50041 gaagtacatg cccgtccggc gatggtggga ttcttcttta ccggcagcgc tgtcattggg
    50101 attctggtaa gtcagtttct cgctgggcgc tctgataagc gcggcgatcg caaatcgctg
    50161 attgtctttt gctgcctgtt aggcgtgctg gcctgcaccc tttttgcctg gaatcgcaac
    50221 tactttgttt tgctattcgt tggcgtcttt cttagcagct ttggctcgac cgctaacccg
    50281 caaatgtttg cccttgcccg tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc
    50341 agctcttttt tacgcgctca ggtttcactg gcatgggtca ttggcccacc gctggcttat
    50401 gcgttagcga tgggtttcag ctttacggta atgtatctga gcgcagcggt agcgtttatt
    50461 gtttgcggcg tgatggtgtg gctgttttta ccgtcgatgc aaaaagagct tccgctggcg
    50521 accggcacgg ttgaagcgcc gcgccgtaac cgtcgcgata cgctactgct gtttgtcatt
    50581 tgtacattga tgtggggctc gaacagcctg tacatcatca acatgccgct atttattatc
    50641 aacgaactgc atcttcccga gaaactggcc gatgtgatga tggggaccgc cgccgggctg
    50701 gaaatcccga cgatgttgat tgccggatat ttcgccaaac gtctgggtaa gcgtttctta
    50761 atgcgcgttg ctgccgtggg tggcgtctgt ttttacgcag gaatgctgat ggcgcattca
    50821 cctgtcattc tgttgggctt gcagctgcta aatgctattt ttattggcat tctgggcggc
    50881 atcgggatgc tctattttca ggatctgatg cccggtcagg cgggttcagc caccacgctc
    50941 tataccaaca cttcgcgcgt gggctggatc atcgcaggat cagtggcggg catcgtcgcc
    51001 gaaatctgga attatcacgc tgtgttctgg tttgcgatgg tgatgattat cgccactctg
    51061 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
    51121 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
    51181 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
    51241 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
    51301 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
    51361 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
    51421 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
    51481 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
    51541 aacggcaaac tgctgctggt taaggatatt gatattcagt cgcccactgc ccgcggtgcc
    51601 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
    51661 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
    51721 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
    51781 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
    51841 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
    51901 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
    51961 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
    52021 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
    52081 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
    52141 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
    52201 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
    52261 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
    52321 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
    52381 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
    52441 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
    52501 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
    52561 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
    52621 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
    52681 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
    52741 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
    52801 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
    52861 aatggccatg tggtgaaaaa cgcggtgctg ggaatggcag aaaaatgttc gccagaactc
    52921 gccgggtgga taaaaaaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
    52981 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
    53041 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
    53101 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
    53161 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
    53221 ttcgtccata tcagtgattg catgcaggat cgcgtatttc gccatgctgc cagaacatta
    53281 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
    53341 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
    53401 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
    53461 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
    53521 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
    53581 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
    53641 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
    53701 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
    53761 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
    53821 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
    53881 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
    53941 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
    54001 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
    54061 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
    54121 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
    54181 gatctgttaa ccgcaccagt ctatgaactg tggatagatc tgcgcgccac cttgtgcatt
    54241 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
    54301 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
    54361 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
    54421 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
    54481 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
    54541 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
    54601 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
    54661 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
    54721 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
    54781 gagctgattt ccagcagcga agctgactgg aatgctttgc agagcgcgct gttgaagctt
    54841 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
    54901 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggcctcgcg ctgtttcttt
    54961 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
    55021 tctttaatca gaaactggtc gaaagtaagg cctttttgtg gctggttgca ttgaccaaca
    55081 atcgcgcttt cgacggttgt tctctgctgg cgatgggaat gttgatgctg agtttctggc
    55141 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
    55201 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
    55261 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
    55321 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
    55381 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
    55441 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
    55501 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
    55561 ccttttttga caaatcacta ccgggaaaaa acaaacattt ccaaaacaaa taactcacag
    55621 taattaacat catcaggctt atttttatag tgaggataat cctgatgatg cgcaccgtgc
    55681 tttcatccat cgcgcgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
    55741 ctcaatcgta ctttaattga ccaatcccgc ttattaactt tctgtatcac tttttcttat
    55801 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatcta gaactcgttt
    55861 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
    55921 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
    55981 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
    56041 cgattttgag atatatcttg cgcgggatcc ccgcgattgc agtagcggtt ctgctttctg
    56101 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
    56161 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
    56221 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
    56281 gcagcactaa aaaaggtatc gattgttctg gttttgtaca acgtacattc cgtgagcaat
    56341 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
    56401 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
    56461 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
    56521 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
    56581 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
    56641 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
    56701 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
    56761 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
    56821 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
    56881 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
    56941 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
    57001 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
    57061 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
    57121 tcaatgaatt gattccggcg ctcaacatta ataaaaacgt cgatatggcg atcttacccg
    57181 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
    57241 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
    57301 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
    57361 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
    57421 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
    57481 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctc tgctattacc
    57541 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
    57601 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgagtaacg ggcctggaag
    57661 tactgctacg ctggcggcat cctgtcgcgg gagaaattcc cccggatgcc ttcattaact
    57721 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
    57781 gcgatgccgc agaattagaa aaagtgctgc cagtaggcgt caaatttggt attaacattg
    57841 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
    57901 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
    57961 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
    58021 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
    58081 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
    58141 acgcggtgct gacgctggcg aaacgcctca atatgctgac ggttgctgaa ggggtcgaaa
    58201 cgccggaaca ggcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
    58261 ttagtcgccc tttaccgctg gacgattttg tccgctggct gaagaaaccg tatacgccgc
    58321 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
    58381 aatgatgagt aaaaggaaat ccattgcaga tgattgtgcg catactgctg ctgtttatcg
    58441 ctctgttcac ctttggtgtg caggcgcagg ctatcaagga aagctatgcc tttgccgtgc
    58501 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
    58561 caaaaggtgg gcagataacg ttgtcagccc tcggcacctt cgataatttc aaccgctatg
    58621 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgcta tttacgactt
    58681 ccgatgacga accaggcagt tattacccgc tgattgctga aagcgcacgc tatgctgacg
    58741 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttcgccca
    58801 ttactgcccg cgatgtagag tttacttttc aaaaatttat gaccgaaggc gtgccgcaat
    58861 ttcgtctggt ctacaaaggc accaccgtca aagccattgc accgttaacc gtgcgcattg
    58921 agttagctaa acccagcaaa gaagatatgc tgagtctgtt ttcgctgccg atatttccag
    58981 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
    59041 gtccgtaccg cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
    59101 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
    59161 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
    59221 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
    59281 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
    59341 cgtttaatat ccaacgtccg gtattcagcg atcgccgggt ccgggaagca atcactctcg
    59401 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
    59461 gttattttca gaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
    59521 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
    59581 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
    59641 acgaaacggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
    59701 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
    59761 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
    59821 ctaaccgcat gcgcagtcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
    59881 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
    59941 cccccggcgt gcaaagcccg gttatcgact cgctgatcaa ccaaattatt gccgcgcagg
    60001 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
    60061 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
    60121 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
    60181 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
    60241 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
    60301 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
    60361 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
    60421 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagac ccagaagtga
    60481 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
    60541 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
    60601 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
    60661 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
    60721 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
    60781 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
    60841 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
    60901 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
    60961 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
    61021 aaggggtaag tgagaaaaac attctctgga aacatgtgtt ccgcaacgcc atgctgctgg
    61081 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    61141 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    61201 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    61261 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    61321 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    61381 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    61441 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    61501 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    61561 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    61621 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    61681 ctggctggga acggatgcca acggcggcga tgtgctggca cgcattctct atggcacgcg
    61741 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    61801 gggggcgcta caaggctatt acggcggtaa agtcgatctc tggggacaac gctttattga
    61861 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    61921 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    61981 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    62041 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    62101 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    62161 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    62221 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    62281 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    62341 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    62401 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    62461 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    62521 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    62581 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    62641 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    62701 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    62761 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    62821 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    62881 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    62941 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    63001 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    63061 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    63121 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    63181 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    63241 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    63301 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    63361 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    63421 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    63481 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    63541 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    63601 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    63661 cattaattct taagccctca ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    63721 cggtacaggc gcaaatattg acgctattga aatcattgca acaaaagcat caactggcct
    63781 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    63841 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc gcaccgcagc
    63901 aggagtatac gcgtcagcta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    63961 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    64021 tggcgattta caaacaggca cggctcacct tcacactcca gcaccgaaca tggaacttcg
    64081 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    64141 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    64201 tcaaccttcg aacctgcgaa accagcagac cagcgaaagt tctgcggtag acgatggacg
    64261 attgagagtt gtaatgaagt cacttatttt ccccggaagc acatttactt cacaatttat
    64321 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    64381 cctcgcctgc atggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    64441 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    64501 gactttttac ataaattgat tttacataaa ataaacatat atcgggggaa tgatagattt
    64561 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
    64621 gttttttcgg tcgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    64681 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    64741 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    64801 gaaactcatc aagaatgacc gccatcgcat tggatgacac catagacacg cagcccacaa
    64861 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
    64921 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    64981 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    65041 caaagtaata accaaagttt tccggtgcaa cgtggttgat ttcaatgtag acaaacggtc
    65101 cggcgctcag gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca
    65161 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
    65221 gctggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
    65281 tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
    65341 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
    65401 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
    65461 acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg gcttacgccc
    65521 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
    65581 actcagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa ttaccggtag
    65641 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
    65701 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
    65761 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
    65821 atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca catggttacc
    65881 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
    65941 ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg agatcttttt cgttatgcag
    66001 ctgcacgcct ttagcaaatt gttctgccgt atcgtcagct acgggtgata ccagcgtcac
    66061 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
    66121 atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg cgtgcagttt
    66181 ccacgctacc ggttcatcta gaaaatagag caccgttggg tgatcagggt cgtccgtgga
    66241 gcaaacataa ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
    66301 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacggac
    66361 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
    66421 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
    66481 aaacggaggt cgggtaatga cccgaatatc tgaactgttg tctgcccagt atagcggtct
    66541 aataacatcc ctcaagggaa aaagatccgt ggcatactat tagcagaata atctacctac
    66601 gcgagaccat gatttttaca cttcgcccat atcagcaaga agccgtggat gccacgctca
    66661 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
    66721 gcctggtgat agcggaactg gcacggctgg ctcgtggtcg cgtactggtg ctggcacacg
    66781 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
    66841 tttttgccgc cgggctgaag cgtaaagaga gccacggtaa agtggtattt ggcagtgtgc
    66901 agtcggtcgc ccgtaatatt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
    66961 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
    67021 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
    67081 aaggttggat ctaccagttc cattatcacg gcatggtacg cagcgatgag aaagcccttt
    67141 tccgtgactg catttatgag ctgcccctgc gttatatgat taaacacagc tatctgacgc
    67201 cgccagaacg actggatatg ccagtagtgc aatatgattt cagccgcttg caggcacaga
    67261 gcaacgggct gttcagcgaa gccgatctca accgtgagct gaaaaaacag caacgtatta
    67321 ccccgcacat catcagccag attatggagt tcgccgaaaa gcgtaaagga gtgatgattt
    67381 tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgcag
    67441 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa aattttaaag
    67501 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
    67561 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
    67621 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
    67681 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
    67741 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
    67801 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
    67861 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
    67921 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
    67981 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
    68041 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
    68101 atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacacctgcc cagcgtaccg
    68161 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatcccg ctgcgctgga
    68221 tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acccccggat tttgtcgtcg
    68281 cccgcatgaa aggccagtac tggcaggtgc gtgaaaaagt gttcgattac gaaggtcgtt
    68341 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
    68401 agtatagaat ctcacccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
    68461 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
    68521 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
    68581 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
    68641 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
    68701 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
    68761 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccc gcggggtttt
    68821 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
    68881 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
    68941 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcagggtat cggtcgccgg
    69001 ctcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
    69061 gctaccggca aacttcgtca actgacgcag tgtgctccgg tctgccggga agctctcttc
    69121 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
    69181 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
    69241 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
    69301 gaaatcatca accgcttgca acagcccacg gttttgtgct ttcgtgttca atccttcgct
    69361 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
    69421 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
    69481 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
    69541 acgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg
    69601 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
    69661 caggcgacca gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
    69721 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
    69781 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
    69841 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
    69901 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
    69961 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
    70021 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
    70081 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
    70141 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
    70201 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
    70261 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
    70321 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
    70381 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
    70441 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
    70501 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact
    70561 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
    70621 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
    70681 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
    70741 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
    70801 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
    70861 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
    70921 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
    70981 acccgatgac ggcgcgacgt tttcttgaga agcatggtct gcttgatgcg caggagtatc
    71041 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
    71101 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
    71161 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
    71221 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
    71281 tctatggtat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
    71341 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
    71401 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
    71461 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccactac gcacaagaag
    71521 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
    71581 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
    71641 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggttgtgatt atcactgccg
    71701 gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac ggtcatctgc
    71761 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gcgctgactg
    71821 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
    71881 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
    71941 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
    72001 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
    72061 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
    72121 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
    72181 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
    72241 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccttt acggttggtt
    72301 tttttatatc tgcaattaat ttgataaaca gaccgtgaca catcacagcc tgtttatttt
    72361 ctgttatcag aacgtccaga ccactcccgc ctgagtattc cacggcgatt ctacccctgc
    72421 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
    72481 cccagcctga taaacgccac gggttccagc cacatcatta ttaaatttgc catcgtcatt
    72541 aactttcaca tggttagaat cggcgtattc ctgacgcacg gcggctttca gccagggttc
    72601 caacgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
    72661 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
    72721 atctgtggta aagccggtaa aggcgagata gggtctaaca ctccacaatc cgtcaaccca
    72781 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
    72841 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
    72901 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tgtagctatc
    72961 aatattgcct ttgccgccgc gatcaaaacc aatatcagaa tgagagtaat caaagaacaa
    73021 gccgcgaatt gtgctgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
    73081 cgtcaatgtt tgctcaaagc cagctcccgc atcagtggtc acgttgttgc gggtgttaat
    73141 tgccgaactc cacatcgccg tatcgtaatt aacgcctttt acgctaccaa gacgctcacg
    73201 cacggtgtcc agttctgcat caaataccag cggttgtgct gctgccatgt tcagcacatc
    73261 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
    73321 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
    73381 gaatgcagca tcaccgccgc ccgtcgttac cagtgtaagg ctatctcctg ctgccgggct
    73441 ggcccccgtg tccgtcacga atattttgaa atcacctgtt gcctgaccga tgacgttgag
    73501 ctgatcgctc tgatgattag ccatatcggt acgcatataa aattgccgtt tcccgacagg
    73561 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    73621 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    73681 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    73741 acgtccgctt ggctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    73801 actaccttac ccaccaacat agcgggtgca taatttattt tcggcttcat gacctcaaga
    73861 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    73921 ccattgttga taaccatatt ttcagagatg gctttagagg aatacgtgat acttccattc
    73981 tgatctgatc gaccgagatc atatacagca tttgcctgca ctttcgtacc agtatccttt
    74041 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg agtgtcctcc
    74101 ataacaatga gcccggagcc atcatccagt tcataatttt ttgacacacc atcctttata
    74161 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    74221 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaat
    74281 cctccctttt cgatagcggc aaagacggca atcccatctt tttcaacaat taagcgcccg
    74341 cctttaatcg tgggatcatt cgccgcg
//
LOCUS       DAEEBB010000004        70301 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEEBB010000004 DAEEBB010000000
VERSION     DAEEBB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.004"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..673)
                     /locus_tag="KLF07_000237"
     CDS             complement(<1..673)
                     /locus_tag="KLF07_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HBD9830368.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="KLF07_000238"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="KLF07_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBD9830369.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="KLF07_000239"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="KLF07_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBD9830370.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="KLF07_000240"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="KLF07_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBD9830371.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="KLF07_000241"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="KLF07_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBD9830372.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="KLF07_000242"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="KLF07_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBD9830373.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALSFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="KLF07_000243"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="KLF07_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBD9830374.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="KLF07_000244"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="KLF07_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBD9830375.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="KLF07_000245"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="KLF07_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBD9830376.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="KLF07_000246"
     CDS             complement(9727..9837)
                     /locus_tag="KLF07_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HBD9830377.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="KLF07_000247"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="KLF07_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBD9830378.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="KLF07_000248"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="KLF07_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBD9830379.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="KLF07_000249"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="KLF07_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBD9830380.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="KLF07_000250"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="KLF07_000250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBD9830381.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="KLF07_000251"
     CDS             complement(14170..14742)
                     /locus_tag="KLF07_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBD9830382.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="KLF07_000252"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="KLF07_000252"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBD9830383.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="KLF07_000253"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="KLF07_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBD9830384.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="KLF07_000254"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="KLF07_000254"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBD9830385.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="KLF07_000255"
     CDS             17911..18759
                     /locus_tag="KLF07_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBD9830386.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="KLF07_000256"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="KLF07_000256"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBD9830387.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="KLF07_000257"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="KLF07_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD9830388.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="KLF07_000258"
     CDS             complement(21671..21871)
                     /locus_tag="KLF07_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBD9830389.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="KLF07_000259"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="KLF07_000259"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBD9830390.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="KLF07_000260"
     CDS             complement(23030..23386)
                     /locus_tag="KLF07_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBD9830391.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="KLF07_000261"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="KLF07_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBD9830392.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="KLF07_000262"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="KLF07_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBD9830393.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="KLF07_000263"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="KLF07_000263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBD9830394.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="KLF07_000264"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="KLF07_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBD9830395.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="KLF07_000265"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="KLF07_000265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBD9830396.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="KLF07_000266"
     CDS             31857..32900
                     /locus_tag="KLF07_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBD9830397.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="KLF07_000267"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="KLF07_000267"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD9830398.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="KLF07_000268"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="KLF07_000268"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD9830399.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="KLF07_000269"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="KLF07_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBD9830400.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38830..39165
                     /gene="eutS"
                     /locus_tag="KLF07_000270"
     CDS             38830..39165
                     /gene="eutS"
                     /locus_tag="KLF07_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBD9830401.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39178..39657
                     /gene="eutP"
                     /locus_tag="KLF07_000271"
     CDS             39178..39657
                     /gene="eutP"
                     /locus_tag="KLF07_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBD9830402.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39632..40333
                     /gene="eutQ"
                     /locus_tag="KLF07_000272"
     CDS             39632..40333
                     /gene="eutQ"
                     /locus_tag="KLF07_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBD9830403.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40330..41133
                     /gene="eutT"
                     /locus_tag="KLF07_000273"
     CDS             40330..41133
                     /gene="eutT"
                     /locus_tag="KLF07_000273"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBD9830404.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41130..42146
                     /gene="pta"
                     /locus_tag="KLF07_000274"
     CDS             41130..42146
                     /gene="pta"
                     /locus_tag="KLF07_000274"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBD9830405.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42185..42478
                     /gene="eutM"
                     /locus_tag="KLF07_000275"
     CDS             42185..42478
                     /gene="eutM"
                     /locus_tag="KLF07_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBD9830406.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42585..42872
                     /gene="eutN"
                     /locus_tag="KLF07_000276"
     CDS             42585..42872
                     /gene="eutN"
                     /locus_tag="KLF07_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBD9830407.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42884..44287
                     /locus_tag="KLF07_000277"
     CDS             42884..44287
                     /locus_tag="KLF07_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBD9830408.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44298..45134
                     /gene="eutJ"
                     /locus_tag="KLF07_000278"
     CDS             44298..45134
                     /gene="eutJ"
                     /locus_tag="KLF07_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBD9830409.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45124..46311
                     /gene="eutG"
                     /locus_tag="KLF07_000279"
     CDS             45124..46311
                     /gene="eutG"
                     /locus_tag="KLF07_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBD9830410.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46628..47854
                     /gene="eutH"
                     /locus_tag="KLF07_000280"
     CDS             46628..47854
                     /gene="eutH"
                     /locus_tag="KLF07_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBD9830411.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47851..49254
                     /gene="eutA"
                     /locus_tag="KLF07_000281"
     CDS             47851..49254
                     /gene="eutA"
                     /locus_tag="KLF07_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBD9830412.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49266..50627
                     /gene="eutB"
                     /locus_tag="KLF07_000282"
                     /pseudo
     CDS             49266..50627
                     /gene="eutB"
                     /locus_tag="KLF07_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50648..51535
                     /gene="eutC"
                     /locus_tag="KLF07_000283"
     CDS             50648..51535
                     /gene="eutC"
                     /locus_tag="KLF07_000283"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBD9830413.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51545..52204
                     /gene="eutL"
                     /locus_tag="KLF07_000284"
     CDS             51545..52204
                     /gene="eutL"
                     /locus_tag="KLF07_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBD9830414.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52217..52717
                     /gene="eutK"
                     /locus_tag="KLF07_000285"
     CDS             52217..52717
                     /gene="eutK"
                     /locus_tag="KLF07_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBD9830415.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52763..53815
                     /gene="eutR"
                     /locus_tag="KLF07_000286"
     CDS             52763..53815
                     /gene="eutR"
                     /locus_tag="KLF07_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBD9830416.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="KLF07_000287"
     CDS             complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="KLF07_000287"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBD9830417.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="KLF07_000288"
     CDS             complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="KLF07_000288"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBD9830418.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55807..56232
                     /locus_tag="KLF07_000289"
     CDS             55807..56232
                     /locus_tag="KLF07_000289"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD9830419.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56219..56668
                     /locus_tag="KLF07_000290"
     CDS             56219..56668
                     /locus_tag="KLF07_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBD9830420.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56729..57304
                     /locus_tag="KLF07_000291"
     CDS             56729..57304
                     /locus_tag="KLF07_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBD9830421.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57400..58299
                     /gene="yfeX"
                     /locus_tag="KLF07_000292"
     CDS             57400..58299
                     /gene="yfeX"
                     /locus_tag="KLF07_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBD9830422.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="KLF07_000293"
     CDS             complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="KLF07_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBD9830423.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59666..59839)
                     /locus_tag="KLF07_000294"
     CDS             complement(59666..59839)
                     /locus_tag="KLF07_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HBD9830424.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59852..61090)
                     /gene="murP"
                     /locus_tag="KLF07_000295"
                     /pseudo
     CDS             complement(59852..61090)
                     /gene="murP"
                     /locus_tag="KLF07_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="KLF07_000296"
     CDS             complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="KLF07_000296"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBD9830425.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62154..63011
                     /gene="murR"
                     /locus_tag="KLF07_000297"
     CDS             62154..63011
                     /gene="murR"
                     /locus_tag="KLF07_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBD9830426.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63140..63931
                     /gene="ucpA"
                     /locus_tag="KLF07_000298"
     CDS             63140..63931
                     /gene="ucpA"
                     /locus_tag="KLF07_000298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBD9830427.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64102..65118
                     /gene="cysP"
                     /locus_tag="KLF07_000299"
     CDS             64102..65118
                     /gene="cysP"
                     /locus_tag="KLF07_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBD9830428.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65118..65951
                     /gene="cysT"
                     /locus_tag="KLF07_000300"
     CDS             65118..65951
                     /gene="cysT"
                     /locus_tag="KLF07_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBD9830429.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65951..66826
                     /gene="cysW"
                     /locus_tag="KLF07_000301"
     CDS             65951..66826
                     /gene="cysW"
                     /locus_tag="KLF07_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBD9830430.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66816..67913
                     /gene="cysA"
                     /locus_tag="KLF07_000302"
     CDS             66816..67913
                     /gene="cysA"
                     /locus_tag="KLF07_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBD9830431.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68047..68958
                     /gene="cysM"
                     /locus_tag="KLF07_000303"
     CDS             68047..68958
                     /gene="cysM"
                     /locus_tag="KLF07_000303"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBD9830432.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68961..69329)
                     /locus_tag="KLF07_000304"
     CDS             complement(68961..69329)
                     /locus_tag="KLF07_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBD9830433.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69434..70285
                     /gene="pdxK"
                     /locus_tag="KLF07_000305"
                     /gene_synonym="thiJ"
     CDS             69434..70285
                     /gene="pdxK"
                     /locus_tag="KLF07_000305"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBD9830434.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16702 a        18348 c        19433 g        15818 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gctcagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggtggtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaagc
    38641 agtctatata atatctcgat attattttat ttatattcat gcgttgcata tgaaagttta
    38701 tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta aacgcgcttg
    38761 ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt ttttcacagg
    38821 cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa acaggtcaca
    38881 ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg cgttccggat
    38941 gcgggcgtaa ttggcattat gacgctaact cccggcgaaa cggcgatgat cgtcggtgat
    39001 ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag cggtgcattg
    39061 gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt cagcggttta
    39121 ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga ggcattcatg
    39181 aaacgaattg cgtttgtggg tagcgtcggg gcaggaaaaa caacgttatt taatgcgttg
    39241 caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga taagggcgat
    39301 attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt aattaccacg
    39361 ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga aagtcggttg
    39421 cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat cagtaaaacg
    39481 gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac gggtttcgaa
    39541 gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact ggtggattat
    39601 ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag cgaatgatat
    39661 tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg ccagcattat
    39721 taccccggaa gcccgtaaag tggcggatct actgggtttt accatcaccg aatgtgatga
    39781 atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa ccgaaagcca
    39841 gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg aaagcctggt
    39901 cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg gcgcaatgca
    39961 gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg gcagcagcgt
    40021 caagtttggt cgttttgatg gcgcacaacc gcattgcgtg ggcttgactg atcttgtgac
    40081 cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg cattcttccc
    40141 gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggagctcc atgttcgcca
    40201 cgaaggcgaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga aaggctccag
    40261 catcgaattt ggtacgacat ccagcgtgaa attcctgtat gtcgcctggc cggctaactg
    40321 gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca tacgctcagc
    40381 gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc ccgggagttg
    40441 ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct gtttgttgac
    40501 gatgaacagc agcagccgca gcccgttcat gggctgacca gtagcgatga acatccgcag
    40561 gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct gactcacctg
    40621 tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc ggttctcgac
    40681 agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg gcagccgtgg
    40741 ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct gggggaaccg
    40801 ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcgact ttctcaccag
    40861 ccgctgcgct atctcgacca cgatcatctg gtgccggaag ccagccacgg gcgcgatgcg
    40921 gcgttgctca atctgctacg taccaaagtg cgggaaaccg aaaccgtagc ggcgcaggtg
    40981 tttatcaccc gcagctttga agtattacgc ccggacatct tgcaggcgct gaaccgcctt
    41041 tcgagcacgg tctacgtaat gatgatcctg agcgtaacga agccgccgct aacggtgaag
    41101 cagatccaac aacgactggg ggaaacgcaa tgattattga acgttgtcgt gaactggcgt
    41161 tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg ctgaaagctg
    41221 cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgctaat ccgtttgaac
    41281 ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg attgatccgc
    41341 atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa
    41401 aaacgccgcc ggatgcgctg gaaaaactta ccgatccgct gatgttcgcc gcagcaatgg
    41461 tcagcgccgg taaagcggat gtctgtatcg cgggcaacct ctcttccacg gcgaatgtgc
    41521 tgcgtgccgg attacgtatt atcggcttgc agccaggctg taaaacgctc tcatccattt
    41581 tcctgatgct gccacagtac agcggtccgg cgttgggctt tgccgattgc agcgtggtgc
    41641 cacagccgac ggcggcgcaa ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg
    41701 ccctcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagtaac ggtagcgccc
    41761 gtcacccctg cgttgccaac gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa
    41821 agctggtggt tgatggcgag ttgcagtttg acgccgcctt cgtgccggaa gtggcggcgc
    41881 aaaaagcgcc tgccagcccg ctacagggca aagccaatgt gatggttttt ccgtcgctgg
    41941 aagccggaaa tattggctac aaaatcgcac aacgactcgg cggatatcgt gccgtcgggc
    42001 cattgataca aggacttgcc gcgccgatgc acgatctctc tcgtggttgt agcgtgcagg
    42061 aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa
    42121 gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata aagaggaaag
    42181 cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga ttgaggcttc
    42241 tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg
    42301 cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg
    42361 tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc cgcgtccgca
    42421 cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca acctgtaatc
    42481 accctgtgtg acgtcgattc gggcgtcacg ctctctcctt tttcatttta ccttctgcgg
    42541 agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg
    42601 tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac aaattgctga
    42661 tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc gccatcgaca
    42721 atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc gcccgccagg
    42781 cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg
    42841 tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc aacaggatat
    42901 tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc
    42961 cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc
    43021 ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc
    43081 aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg gcatgggccg
    43141 cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg
    43201 tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa acgcgccctg
    43261 gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa ttaataacgc
    43321 catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa
    43381 agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga
    43441 aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt
    43501 tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa
    43561 acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag tggtggatga
    43621 aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa
    43681 catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg atgaactgat
    43741 gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc agcagctgca
    43801 accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt
    43861 tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg
    43921 tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc
    43981 ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact
    44041 ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa acatgaacca
    44101 gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgcgca ttgccgggct
    44161 ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg gtgaaggggt
    44221 aactagcgtg cgtacgtttg tccgtctgcg tcgctgtgta ttagtcgatg cgtttcgcat
    44281 tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt ctgcaaacgg
    44341 cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg
    44401 atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag tcggtggcgg
    44461 tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg
    44521 tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc cgttttagcc
    44581 atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac gtgctggagt
    44641 ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg gatctgctgc
    44701 aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt gccatcgtga
    44761 aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac atctctctga
    44821 ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag cgcggtcacg
    44881 gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggcggac attgtcgccc
    44941 ggcatattga agggcagggc attactgatt tatggctggc gggcggctct tgtatgcaac
    45001 cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc
    45061 acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc
    45121 tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa
    45181 cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc ggtgagcagt
    45241 tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc agacagcttt
    45301 ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc
    45361 atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg
    45421 gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg
    45481 gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct ggcagagatg
    45541 tcagaaacca gcgttctgca accgcgcttg ccgctgattg ccattccaac gaccgccgga
    45601 accggctctg aaaccaccaa tgtaacggtg attatcgacg cggtgagcgg gcgcaagcag
    45661 gtgttagccc atgcctcgct gatgccggat gtggcgattc tcgacgccgc gttgaccgaa
    45721 ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg cgttaaccca tgccattgaa
    45781 gcatacagcg ccctgaacgc tacaccgttt accgacagtc tggcgattgg tgccattgcg
    45841 atgattggca aatcgctgcc gaaagcggtg ggctacggtc acgaccttgc cgcgcgcgag
    45901 agcatgttgc tggcttcatg tatggcggga atggcgtttt ccagtgcggg tcttgggttg
    45961 tgccacgcga tggcgcatca gccgggcgcg gcgctgcata ttccacacgg tctggcgaac
    46021 gccatgttgc tgccaacggt gatggaattt aaccggatgg tttgtcgtga acgctttagt
    46081 cagattggtc gggcactgcg aactaaaaaa tccgacgatc gtgacgctat taacgcggta
    46141 agtgagctga ttgcggaagt tgggattggt aaacgactgg gcgatgttgg tgcgacatct
    46201 gcgcattacg gcgcatgggc gcaggccgcg ctggaagata tttgtctgcg cagtaacccg
    46261 cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg cggcgcaata aatgccggat
    46321 gcgacgcttg ccgcgtctta tccagcctac gggattgcac atgtagggcg gataaggcgt
    46381 tcacgccgca tccgccagtg gcgcggtgca attgccggat gcgatgcttg ccgcgtctta
    46441 tccggcctac gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    46501 gcgcggtgca attgccggat gcgacgcttg ccgcgtctga tccggccttc gggattgcac
    46561 atgtagggcg gataaggcgt ttacgccgca tccgccaata aataacaact aacagggagt
    46621 aaaggcgatg ggaattaacg aaatcatcat gtacatcatg atgttcttta tgctgatagc
    46681 tgccgtagac aggatcctgt cgcagttcgg cggttctgct cgtttcctcg gtaagttcgg
    46741 taaaagtatc gaaggatcag gcggtcagtt cgaagaaggc tttatggcaa tgggcgctct
    46801 gggtctggcg atggtcggta tgaccgcgct ggcaccggta ctggctcacg tactcgggcc
    46861 ggtaattatc ccggtttacg aaatgctcgg cgcaaacccg tcaatgttcg ccggaacgct
    46921 gctggcgtgc gatatgggcg gcttcttcct cgccaaagag ctggcgggcg gcgacgtagc
    46981 agcgtggcta tactctgggt taattcttgg gtcgatgatg gggccaacga ttgtgttttc
    47041 cattccggtg gcgctcggca ttatcgaacc ttctgaccgt cgttatctgg cgctcggcgt
    47101 gctggcgggc attgtgacca ttccgattgg ctgtattgct ggtggtctgg ttgctatgta
    47161 ctccggtgtg cagatcaacg gtcagccggt ggaattcact ttcgccctga tcctgatgaa
    47221 catgatcccg gtgatcattg ttgcgattct ggtggcgctg gggctgaaat tcatcccgga
    47281 aaaaatgatc aacggcttcc agatcttcgc caaattcctc gttgcattga tcaccctcgg
    47341 tcttgctgcc gcggtagtta agtttctgct tggctgggaa ctgatccccg gtctggaccc
    47401 tatctttatg gcccctggcg ataaacccgg tgaagtgatg cgcgccattg aagttatcgg
    47461 ttctatctcc tgcgttctgt taggggcgta tccaatggtg ctgctgctga cccgctggtt
    47521 tgaaaaaccg ctgatgagcg tcggtaaagt gctgaatatg aacaacatcg cggcagccgg
    47581 catggtggca acgcttgcca acaacatccc gatgttcggc atgatgaagc agatggatac
    47641 ccgcggcaaa gtcatcaact gcgccttcgc cgtttccgct gctttcgccc tgggcgacca
    47701 cttaggcttc gccgctgcca acatgaacgc catgatcttc ccgatgattg tcggcaagtt
    47761 gatcggcggc gtaacggcga ttggcgtggc gatgatgctg gtgccaaaag aagacgcgac
    47821 cacggctaaa accgaagcgg aggcacaatc gtgaacactc gccagctatt gagcgtcggt
    47881 atcgatatcg gcaccaccac cacccaggtg attttctccc acctggagct ggttaaccgt
    47941 gcggcggtgt cgcaggtgcc gcgctacgaa ttcattaaac gcgaaattag ctggcaaagc
    48001 ccggtgttct ttacccctgt cgataaacag ggtggtttaa aagaagcgga actgaaaacc
    48061 ttaatcctcg agcaatatca ggctgcgggc attgcgccgg aaagcgttga ttctggtgcc
    48121 atcatcatca ccggtgaaag cgcgaaaacc cgcaatgctc gtccggcggt gatggtgctc
    48181 tcccagtcgc tgggcgattt tgtcgttgcc agcgccgggc cgcacctcga atccgtcatt
    48241 gccggtcacg gtgccggggc acaaaccctt tctgaacaac ggctgtgtcg ggtactgaat
    48301 attgacatcg gcggtggcac tgcgaactac gccctgttcg atgccggaaa aatcagcggc
    48361 actgcctgcc tcaacgtcgg tggtcgcctg ctggaaaccg acagccaggg gcgcgtggtt
    48421 tacgctcata aaccggggca gatgattgtg gatgagtgct tcggcgcagg caccgacgcc
    48481 cgttcgctga ccggcgcgca gctggtgcag gttacccggc ggatggcaga actgattgtc
    48541 gaagtgattg acggaacgct ctcgccgctc gcgcaggcat tgatgcaaac cggtttgctg
    48601 cccgcaggtg ttacgcccga aatcattacg ctttctggcg gcgtgggcga atgttatcgc
    48661 caccagcccg ccgacccgtt ctgttttgcc gatattggcc cgctgctggc aacggcgctg
    48721 catgaccatc cgcgcctgcg tgagatgaac gtacagtttc cggcgcaaac cgtgcgcgcc
    48781 acggtgattg gcgcgggcgc gcataccctt tcgctctctg gcagcacaat ctggcttgag
    48841 ggcgtacaac tgccgctgcg caatttgccg gtggcgatcc cgattgatga aacggatctg
    48901 gtgagtgcct ggcaacaggc gctgcttcag ctggatcttg atcccaaaac tgacgcgtac
    48961 gtgctggcgc ttcccgcctc gctgcctgtg cgttacgccg cggtactgac ggtcatcaac
    49021 gcgctggtcg atttcgtcgc gcgttttcca aatccgcatc ccctgctggt ggtggccggg
    49081 caggactttg gtaaagctct gggcatgttg ttgcgcccac agctacaaca actcccgttg
    49141 gcagtcattg acgaagtgat tgtccgcgcg ggggactata tcgacattgg tacgcctctt
    49201 tttggcggat cggttgtgcc ggtgacggtg aaatcactcg catttccttc ctgagggaac
    49261 gacttatgaa actaaagacc acattgttcg gcaatgtata tcagtttaag gatgtaaaag
    49321 aggtgctggc taaagccaac gaactgcgtt cgggggatgt gctggcgggc gttgcagcgg
    49381 caagctcaca ggagcgcgtg gcggcaaagc aggtgttgtc ggaaatgacc gtagcggaca
    49441 tccgcaataa tccggtgatt gcctatgaag atgactgcgt gacgcggctg attcaggacg
    49501 atgttaacga aacggcctac aaccagatta aaaacaggag catcagcgaa ctgcgtgagt
    49561 atgtgctgag cgatgaaacc agcgtggacg acattgcctt tacccgcaaa gggctgacct
    49621 cggaagtggt cgcggcggta gcgaagattt gctccaacgc ggacctgatc tacggcgcga
    49681 agaaaatgcc ggtaatcaaa aaggccaata ccaccatcgg tattccgggc accttcagcg
    49741 cccgtttgca gccaaatgac acccgtgacg acgtgcaaag tatcgctgca caaatctacg
    49801 aagggctttc cttcggggtg ggcgatgcgg tgatcggcgt taacccggta actgacgacg
    49861 tggaaaactt aagccgcgtg ttggacacca tttacggcgt gatcgacaaa ttcaacatcc
    49921 caactcaggg ctgcgtactg gcgcacgtca ccacccagat cgaagcgatt cgtcgcggcg
    49981 cgcctggcgg actgattttc cagagtatct gtggcagcga aaaagggctg aaagagtttg
    50041 gcgtggaact ggcgatgctc gacgaagcgc gcgcagtggg tgctgagttc aaccgtatcg
    50101 ccggggaaaa ctgcctctac ttcgaaaccg ggcaaggttc ggcgctgtct gctggcgcta
    50161 acttcggcgc agactaggtg acgatggaag cacgtaacta tgggctggcg cgtcattacg
    50221 atccgtttat cgtcaacacc gtggtcggct ttattgggcc ggagtatctc tacaacgacc
    50281 gccagattat ccgtgctggc ttagaagatc actttatggg caagctgagc ggcatctcta
    50341 tgggctgtga ctgctgttac accaaccacg ctgatgctga ccagaacctc aacgaaaacc
    50401 tgatgatcct gctcgccacc gcaggctgca actacatcat ggggatgccg ctgggcgatg
    50461 acatcatgct caactatcag accaccgcat tccacgacac tgccactgtg cgtcagttac
    50521 tcaacctgcg cccgtcaccg gagtttgaac gctggctgga aagcatgggc attatggcaa
    50581 acggtcgcct gaccaaacgg gcgggcgatc cgtcactgtt cttctgatga cgcggagata
    50641 acacatcatg gatcaaaaac agattgaaga aattgtacgc agcgtgatgg cgtcaatggg
    50701 acaagcggcc cccgcgccgt cagaagcaaa gtgcgccacc actaactgtg cggcaccggt
    50761 gacctcggaa agctgcgcgc tggatttagg ttctgcggaa gcaaaagcgt ggattggtgt
    50821 tgaaaacccg catcgcgcag acgtattaac agaactgcgc cgcagcaccg tggcccgcgt
    50881 ttgtaccggt cgtaccggtc cgcgtccacg tacgcaggcg ctgctgcgtt tcctggccga
    50941 tcactctcgt tcgaaagata ccgtactgaa agaagtgccg gaagagtggg tgaaagcgca
    51001 gggcttgctg gaagtacgct ccgaaattag cgacaaaaat ctctacctga ctcgcccgga
    51061 tatgggccgc cgcctgtgtg cagaagctgt tgaagcgctg aaagcacagt gtgttgctaa
    51121 tccggacgta caggtcgtta tttctgatgg cctgtcaact gatgccatca ccgtgaacta
    51181 cgaagagatc ctgccgccgc tgatggcggg cctgaaacag gccgggctga aagtcggcac
    51241 gccattcttt gttcgttatg gtcgcgtgaa gattgaagat cagatcggtg agatcctcgg
    51301 cgcgaaagtg gtgatcctgc tggtggggga acgtccgggg ttgggtcagt cagaaagcct
    51361 ctcctgttac gccgtttact cgccgcgtat ggcgaccacc gttgaggccg atcgcacctg
    51421 catctctaac attcaccagg gcggcacgcc gccggttgaa gccgcagccg tcattgtgga
    51481 tttggcgaaa cgtatgctgg agcagaaagc atccggcatc aacatgaccc gataaggagg
    51541 catcatgcca gctttagatt tgattcgacc gtcggtaacc gctatgcggg tgattgcctc
    51601 cgttaactct gaatttgcgc gtgaactaaa attgccgccg catattcgta gcctcgggct
    51661 gatttctgct gattccgatg acgtcaccta cattgcggct gacgaagcga ccaagcaggc
    51721 gatggtcgaa gtggtatatg gccgctcgct gtatgcggga gcagcacacg gaccgtcacc
    51781 gactgccggt gaagtgctga ttatgcttgg tgggccaaac ccggcggaag tgcgtgctgg
    51841 tctggatgcg atggttgcgc atattgaaaa tggcgctgct ttccagtggg ctaatgacgc
    51901 agaaaacacc gcattcctgg cacatgtagt ttcgcgtacc ggttcttatc tttcatcaac
    51961 cgccgggatc acgcttggcg atccgatggc gtatctggtg gcaccgccgc tggaagcgac
    52021 ctacggcatt gatgcagcgt tgaaatctgc cgacgtacag ctggtgacct atgtcccgcc
    52081 accgtctgaa accaactact cggcagcatt tttaaccggt agccaggccg cgtgtaaagc
    52141 agcctgtaac gcctttaccg atgcagtgct ggaaatcgcg cgtaatccaa tccagcgtgc
    52201 gtaacggagg ttgccgatga tcaatgcact gggattgctg gaagtggacg gcatggtcgc
    52261 cgcgatagat gcagcggatg ccatgctcaa agcagctaac gttcgtctgc tcagtcacga
    52321 agtgcttgac cctggtcgct taacgctggt ggtggaaggc gatctggcgg cgtgtcgtgc
    52381 ggcgctggac gcaggttgtg ctgccgcgat gcgtaccggg cgtgtcatca gccgcaagga
    52441 gatcggtcgg ccagacgatg acacccagtg gctggttact ggctttaacc gccagccgaa
    52501 gcaacccgta aaggaacccg acgcgccagt tatcgtcgcg gaatctgctg acgagttgtt
    52561 ggcgctgtta acatcagtac gtcagggaat gacggcagga gaagtggctg cccactttgg
    52621 ctggccgctg gaaaaagcca gaaatgcgct cgaacagctc ttttctgccg ggacgttacg
    52681 taaacgcagt agtcgttatc gtctcaagcc ccattaacct gtcggaggtg ccgggtgtcc
    52741 tacaacaccc ggcattaaca tcatgaaaaa gacccgtaca gccaatttgc accatcttta
    52801 tcatgaaccc ttacccgaaa acctgaagct cacgccgaag gtcgaagtgg ataatgttca
    52861 tcaacgacag acaacggatg tctatgaaca tgctttgacg attaccgcct ggcagcagat
    52921 ttacgatcag ctgcatccgg gcaagtttca tggtgaattt acggaaattc tgctcgatga
    52981 tattcaggtt tttcgtgaat acaccggtct ggcgctgcgt cagtcgtgcc tggtctggcc
    53041 gaactcgttc tggtttggca ttccggcgac gcgcggtgag cagggattta tcggttcgca
    53101 atgtctggga agcgcagaaa tagcgacgcg ccccggtgga accgagtttg aattaagtac
    53161 gccggacgat tatacgatcc tgggcgtagt gctgtctgaa gatgtcatca cccgtcaggc
    53221 taactttttg cacaatccgg atcgggtatt acatatgctg cgtagccagt cggcgctgga
    53281 agtgaaagag cagcataaag ccgcgctgtg gggctttgtc caacaggcgc tggcgacgtt
    53341 ttgcgagaac ccggaaaatc tccatcagcc tgcagtgcga aaagtgctgg gggataattt
    53401 gctaatggcg atgggggcga tgctggaaga cgcgcaacca atggtgacgg cggaaagcat
    53461 cagtcatcag agttaccgta gattactttc ccgcgcccgt gaatatgtgc tggaaaatat
    53521 gtctgagctg gtgacggtgc tggacttgtg taatcaactg catgtcagtc gccgcacgct
    53581 acaaaacgcg tttcacgcta ttttaggcat tggcccaaac gcgtggctga aacgcattcg
    53641 cctgaacgcc gtacgccgcg aactgataag cccgtggtcg caaagcacaa cggtaaaaga
    53701 cgccgccatg cagtggggat tctggcatct ggggcaattt gccacggatt accagcagct
    53761 gtttgccgag aagccgtcgt tgacgttgca tcagcggatg cgggaatggg ggtgagggag
    53821 ttacacccaa tccctgacct taataaactc acttaacgcc gcttctgggc tgccatcttt
    53881 tggctgataa tcatactccc agcgtaccag tggcggcatt gacatcagga tcgactcggt
    53941 gcgcccgcca gtttgcaggc caaacagagt gccgcgatcc cagaccagat tgaactcgac
    54001 ataacgaccg cgacggtaga gctgaaaatt gcgctcgcgc tcgccgtagg ccatcgcttt
    54061 acgtcgctct acaattggta aataagcgtc ggtgtagcct ttgcctaccg cctgcataaa
    54121 ggcaaaacag tggtcgaaat ctggcgtgtt cagatcatca aagaacagcc cgccaatacc
    54181 gcgctgttcg ttgcgatgtt tgaggtagaa gtaatcgtcg caccactttt tgtaacgggg
    54241 ataaacgtct tcaccaaatg gcaggcacag gtcacgggcg gtgcggtgcc agtgaatggc
    54301 gtcttcttca aaaccataga aaggggttaa atcgaagccg ccgccaaacc accagacggg
    54361 ctctgcaccc ggtttttcgg caataaaaaa acgcacattc gcgtggctgg tgggaacata
    54421 cgggttatgc ggatgcacta ccagtgaaac gcccatcgcc tcgaaactgc gcccggcaag
    54481 ttccgggcga tgagcggtgg cggaagcagg catcgcctcg ccgtggacgt gagaaaagtt
    54541 gacgcctgcc tgttcgaaaa caccaccatt acgcaacacc cgactacgcc cgccgccgcc
    54601 agcttcgcgc tgccaactat cttcgacaaa ttctgcgcca tcgacggcgg tcagctgctg
    54661 acaaatcgta tcctgaaggt tgagcagaaa ctgtttaacc tggtgtgcgt cgggtttcat
    54721 aacttatcgc tttttcgaat gtgctttctg gttgtcgaac cagtggaaat aactgatcac
    54781 gccttcagca atcgctgtgg cgattttctg acgaaacgcc accgtgccta acagccgctc
    54841 ttcttccggg ttggtgataa acgaggtttc caccagcacc gaaggaaccg acggcgattt
    54901 caacaccaca aacgcggctt gttcggtgtt gcggctgtgc agtttatgca ccggcttaat
    54961 ctttttcaga atatgcgagc caagcgtcag gctgttttta atagtatcgg tttgcaccag
    55021 atcaaacagc acttgctgca ataggtgatc cttgtcagtc gcctttttac cggcaacttc
    55081 atcggcgcgg ttttcgcgtt cagacaggta tttcgccatt gcgctacttg ccccacggtt
    55141 agagagggca aataccgaag caccggcagc tttcgggttg gtaaagccat cggcatgaat
    55201 tgacataaac agatctgcgc catgtttatg ggcgatttca acgcgatcgt aaagtgggat
    55261 aaacgtatcg ccagaacgcg ttaaacgcgc atcaatccca tgattacgca aaatggaacg
    55321 gacgttttta gcaatcgcca gcaccacatg tttttctttc gaaccgttgc gtccgatcgc
    55381 tccggtatca attccgccgt ggcctggatc gagaacaacg acacgtttgc cgccagattt
    55441 tttggctttc ggcttgctgt gtccgttgct ggtttttaaa ggttcgtctt tggcgatggc
    55501 ttgcgacatt cctgacaacg tcagggcagc caaaccggct ttcagcacct ggcggcgcga
    55561 agtgagtgtt tttagtggtt taaaagtgct catacggcct gagttgtaat aataaagttc
    55621 cagatgttat atcgtatcgc gtaatccgtt acgacggttt gatgttgaga attgttttac
    55681 ttttcatttc aatagttgac aactccccat tatgccactt tttcaaccga ttttcgtcag
    55741 atattggcta attcgaccgt tcacgccata atcagccttt taacccgcaa aaaggtaacg
    55801 aataccatgg agatacgcgt atttcgccag gaagatttcg aagaggtcat caccctttgg
    55861 gagcgttgcg acttgctgcg tccgtggaac gatccggaaa tggacatcga gcgtaagatg
    55921 aaccatgacg tcagtttgtt tttggtcgct gaggtaaacg gtgaagtggt cggaacggtg
    55981 atgggcggtt atgacgggca tcgcgggtct gcttattatc ttggcgtgca tccagagttt
    56041 cgtgggcgtg ggattgccaa tgcgttgctt aatcggctgg agaaaaagct gattgctcgt
    56101 ggctgcccga aaattcagat caatgtgccg gaagataacg acatggtgct cggaatgtat
    56161 gaacgcctgg gatatgaaca cgccgacgtg ctgagtctgg gtaagcgttt gattgaagat
    56221 gaagagtact gagttccatc ctgtccatta tgatgcgcac ggacgcctgc gtttaccctt
    56281 gctcttctgg cttgtgctat tgcttcaggc gcgaacctgg gtgctgtttg tcattgccgg
    56341 tgcgtcgcgc gagcagggta ctgcgctgct aaatctgttt tatcccgatc acgataattt
    56401 ctggctgggg ctgattccag gcattccggc ggtgctggcg tttttgttga gcggtcggcg
    56461 ggctacattt cctcgcactt ggcatgtgct ctattttctg ttgctgttgg cgcaggtggt
    56521 tttactttgc tggcaaccgt ggctgtggct gaacggtgaa tccgttagcg gtatcggtct
    56581 ggcgctggta gtggcggata ttgtggcgtt aatctggctg ctgaccaatc gacgtttacg
    56641 cgcttgtttt aatgaagaaa aagaataacg gcactttttg gtgaatttgc actccaagca
    56701 acgttattga ataaccaaag gaagtgacat gaaatcgctg cgtttaatgt tatgcgctat
    56761 gccgttgatg ctgaccggct gttccacgat gtcgtcagtt aactggtcgg ccgctaaccc
    56821 gtggaactgg tttggatcgt ccaccaaagt gagcgagcag ggcgtgggtg aattaacggc
    56881 gtccacacca ctgcaagaac aagccattgc tgatgcgctt gatggcgatt atcgcctgcg
    56941 cagcggaatg aaaaccgcga acggcaacgt ggtgcgcttt tttgaagtga tgaaaggcga
    57001 caacgtggcg atggtgatta acggcgatca aggcacgatc agtcgcattg acgtgctgga
    57061 tagcgatatt cctgctgaca ccggtgttaa aatcggtaca ccgtttagcg acctttacag
    57121 caaagcattt ggcaattgcc aaaaagctga tggtgatgat aatcgtgccg tcgaatgtaa
    57181 agccgaaggc agtcaacata ttagctacca gttcagcggg gaatggcgtg gtcctgaagg
    57241 gttaatgcct tcggacgata ccctgaaaaa ctggaaagtc agtaaaatta tctggcggcg
    57301 ttaatttgcg tctgaacaaa ccgccgccgc gaaaaaacac gtaaaatagc gctcaacaat
    57361 gccacggatt gcgtggcatt cttattttca ggaggaacaa tgtctcaggt tcagagtggc
    57421 attttgccag aacattgccg cgcggcgatt tggatcgaag ccaacgtgaa aggggaagtt
    57481 gacgccctgc gtgcggccag taaaacattt gccgacaaac tggcaacttt tgaagcgaaa
    57541 ttcccggacg cgcatcttgg tgcggtggtt gcctttggta acaacacctg gcgcgctctg
    57601 agcggcggcg ttggggcaga agagctgaaa gattttccgg gctacggtaa aggccttgcg
    57661 ccgaccaccc agttcgatgt gttgatccac attctttctc tgcgtcacga cgtaaacttc
    57721 tctgtcgccc aggcggcgat ggaagccttt ggtgactgca ttgaagtgaa agaagagatc
    57781 cacggcttcc gttgggtgga agagcgtgac ctgagcggct ttgttgacgg tacagaaaac
    57841 ccggcgggtg aagagacgcg tcgcgaagtg gcggttatca aagacggcgt ggatgccggc
    57901 ggcagctatg tgtttgtgca gcgttgggag cacaacctga agcagctcaa ccggatgagc
    57961 gttcacgatc aggagatgat gatcgggcgc accaaagagg ccaacgaaga gattgacggc
    58021 gacgaacgtc cggaaacctc tcacctcacc cgcgttgatc tgaaagaaga tggcaaaggg
    58081 ctgaagattg ttcgccagag cctgccgtac ggcaccgcca gtggcactca cggtctgtac
    58141 ttctgcgcct actgcgcgcg tctgcataac attgagcagc aactgctgag catgtttggc
    58201 gataccgatg gtaagcgtga tgcgatgttg cgtttcacca aaccggtaac cggcggctat
    58261 tacttcgcgc cgtcgctgga caagttaatg gcgttgtaag cgtttctgat tcgatgatta
    58321 aaggccagcc agtaacaaaa ctgactggcc tttttattac ttttgcttca acgccgcata
    58381 cacctgatca actacccaac cataagttgc aatcggtaac tgaccgcttt cgaacatatt
    58441 gggatttttt tgtggatcgg caactggcga atggggcttg ttgcttaaca tcacaatcgc
    58501 catatgattg accggatcga taacggttac tgtcccggtc cagccagtgt gaccgtaggt
    58561 ttgcgggctt gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat tcacgcgcca
    58621 gccgaggcca aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc
    58681 actgaacagc tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc
    58741 aatatcgccg gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa
    58801 ggctttttca tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat
    58861 cacgccatcg cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc
    58921 tttcagcagc gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc
    58981 aacatagcgg tcaagcggct gaccggtaac cgactcgacg ataaatccaa gcagcatata
    59041 atcgacatcg ctgtagatat gtttgctgcc gggctgatat tccagcggcg tgcgcttgat
    59101 catctccagc gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg
    59161 gtattgcgga tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt
    59221 gtttttgcct ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat
    59281 ccgatcgtca ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc
    59341 gtacattttg gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg
    59401 ttccatcaac acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat
    59461 ctgattatct ttaatgatca gcaggtttac gccgggataa ccgacatcaa tttgctggct
    59521 aatccagcga tccatctggt taagccgttc gacgttaaac cctgctttct ctggcgagct
    59581 ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag
    59641 atagagcatt gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg
    59701 tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccatgg
    59761 ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc taccagacca gacggcccaa
    59821 aggcgctgtt taagcccatc ggcagacccc atcaggctat caggccgata aacaaaccgc
    59881 ccgccgcgcc gcctaaacag gcggtaacaa acggcttcat gcggggcagg gtgacaccgt
    59941 aaatcagcgg ttcaccaacg cccagcaggc cgggaataat cgccccgcgt acctgactgc
    60001 gtagcgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg
    60061 cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctagctgtcc attaacgcga
    60121 ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg
    60181 ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt
    60241 cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca
    60301 gggtgattaa cgaggtcagc agcatgtcga gatcgtccga cataaagcgg cgcaccatgc
    60361 cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat
    60421 cgatgggcag accaaagaaa tcgtgaaaac cggcgtagtc accggtggtt gcagcggggt
    60481 tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg
    60541 cctgggcggc gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca
    60601 ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca
    60661 ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca
    60721 gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg
    60781 cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc
    60841 ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct
    60901 gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat
    60961 caaccagtga actgtcatgt acacccagac gcaggtgcgt catacagtta ccacaactgg
    61021 cgatatttcc cagtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct
    61081 ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca
    61141 ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac
    61201 agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt
    61261 ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca
    61321 tcaggttgcc gaacactttg ccggatttaa tcatcagccc ggtggaaagc atattgagca
    61381 ccagtttctg cgctgtgcct gctttcattc gcgaagaacc agtgaccact tcggcaccta
    61441 caatcggtgt aatggcaaac tcggcggtgg tggaaacggc actccccgga ttacaggaaa
    61501 ttcccactgt gcggcaaccg agctggcggg cgtattccag tccggcaatc acatacggcg
    61561 tacgaccgct ggcggcaata ccgaccacca catcctgtgc cgttaaattt atatttttca
    61621 gatcgttaat gccgccttct cggctatctt ccgtgccttc caccgcgtgc tgaatggcat
    61681 attcgccgcc agcaatcaaa ccaaccacca gacccggttt cacgccgtag gtgggcggac
    61741 attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac
    61801 gaccgccgcc gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagga
    61861 cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca
    61921 gcgtcgatac gcggtcaatt tcagccgagg cggtgttcga gccttcagta atcatctttt
    61981 caagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc
    62041 atatatcccg aattgacaga cttaaaggaa tcattgagtc tacacttgca gagtgtgaag
    62101 agcgtcataa gaacttaagc ggaatttttg ctgccagccc gggagagtgg aagatgttgt
    62161 acctgacgaa aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg
    62221 actttctgcg ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac
    62281 agctcggcat cagtcagtcg agcattgtga aatttgccca gaagctcggc gcgcaaggat
    62341 ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg
    62401 ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc
    62461 gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgtgcgttg ttcgactacg
    62521 ctcggctgca aaaaatcatt gaggtcatca gtaaagcacc gtttatccag ataacgggcc
    62581 tgggcgggtc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc
    62641 gcgtggccta tgaagccgac acacacgtac aagccaccgt ttcccaggcg ttgaaaaaag
    62701 gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag
    62761 aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc
    62821 tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct
    62881 cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg
    62941 tgcaactcaa tgatgtggag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac
    63001 gcctgaaatg atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat
    63061 ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat
    63121 taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc
    63181 attgcaggga attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat
    63241 cttgctggat atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca
    63301 tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa
    63361 acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg
    63421 tctgggcagt ttcctcgata tgagcgatga cgatcgcgat ttccatattg acatcaatat
    63481 taaaggcgta tggaacgtca cgaaggcggt gctgccggag atgattgccc gcaaagatgg
    63541 tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc
    63601 gtacgcctta acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc
    63661 gcagtctggt attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga
    63721 aagcattgcc cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa
    63781 agcaatcccg ctgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct
    63841 cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac
    63901 actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg
    63961 gggaggattt cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg
    64021 gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca
    64081 ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgcgctg
    64141 gtcgcttctc tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat
    64201 gacgtctccc gcgagctgtt tgccgccctg aacccgccgt ttgagcaaca atggaccaaa
    64261 gaaaacggcg gcgacaaact gacaataaaa caatctcatg ccgggtcatc aaaacaggcg
    64321 ctggcaattt tgcagggctt aaaagcagac gttgtcactt ataaccaggt gactgacgta
    64381 caaatcctgc atgacaaagg caagctgatc ccggcggact ggcaaacgcg cctgccaaat
    64441 aatagctcgc cattctactc caccatgggc ttcctggtgc gtaagggcaa cccgaagaat
    64501 atccacgact ggaacgacct ggtgcgctcc gatgtgaagc tgattttccc gaacccgaaa
    64561 acgtcgggta acgcgcgtta cacctatctg gcggcatggg gcgcagcgga taaagctgac
    64621 ggtggcgata aagccaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg
    64681 ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg agcgcggcct gggcgatgtg
    64741 ctgatcagct tcgagtcgga agtgaacaac atccgtaaac agtatgaagc gcagggtttt
    64801 gaagtggtga ttccgaaaac caacattctg gcggaattcc cggtggcgtg ggttgataaa
    64861 aacgtgcagg ccaacggtac ggaaaaagcc gccaaagcct accttaactg gctctacagc
    64921 ccgcaggcgc aaaccatcat caccgactat tactaccgcg tgaataaccc ggaagtcatg
    64981 gataaactga aagacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc
    65041 tcctggccgg aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta
    65101 gcggcggggc gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct
    65161 taagcctcgg caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc
    65221 tggtgatgca gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc
    65281 aggtagtagc ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg
    65341 gcgtttttgg cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc
    65401 tgcttgatgc gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc
    65461 tagcctcgct cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca
    65521 aagtcaccta tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg
    65581 tggtacgtac cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcag
    65641 aaacgcttgg cgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gaactttctc
    65701 cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg
    65761 tgatttttat cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg
    65821 tgcgcttaca ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg
    65881 catctctgct gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg
    65941 taggtcatta atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg
    66001 gggcaaatgg tttctgattg gcatcgggat gctggtatcg gcgttcatcc tgctggtgcc
    66061 gatgatttat atcttcgtac aggcgttcag caaagggctg atgccggttt tacagaatct
    66121 ggccgatccg gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt
    66181 accggtaaac ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc
    66241 tggacgccag ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc
    66301 cggtctggtg tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga
    66361 acataatctg caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac
    66421 gtgtccgttt gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggaaga
    66481 cgaagcggcg attttgcttg gcgcgtctgg ctggcagatg tttcgccgcg tcacattgcc
    66541 gaacattcgc tgggcgctgc tttatggcgt ggtgttgacc aacgcccgcg caattggcga
    66601 gtttggcgcg gtgtcggtgg tttccggctc gattcgcggc gaaaccctgt cgctgccgtt
    66661 acagattgaa ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact
    66721 gttaacgctg atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga
    66781 gaatcaggaa aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta
    66841 agaagtcgtt tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc
    66901 agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg
    66961 ccgggctgga gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc
    67021 tgcacgcgcg cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata
    67081 tgacggtatt cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gatcgcccga
    67141 atgccgcagc catcaaagcg aaagtgacaa aattgctgga gatggtgcag cttgctcatc
    67201 tggcggatcg ttatccggca cagctttccg gcggccagaa acagcgcgtg gcgctggcgc
    67261 gcgcgctggc tgtagaaccg caaattctgc tgcttgatga accgtttggc gcgctggatg
    67321 cgcaggtgcg taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca
    67381 ccagcgtgtt cgtgacccac gaccaggaag aagcgacgga agtagctgat cgtgtagtgg
    67441 tgatgagcca gggcaatatt gaacaggctg atgcgccgga gcaggtatgg cgcgaaccag
    67501 cgacccgttt tgtgctcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg
    67561 gcgggcagtt ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg
    67621 ggccggtgga tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg
    67681 attcgccgct gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag
    67741 tggtgcagcc gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg
    67801 ccccgcagcg tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg
    67861 acgagcgtat cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg
    67921 agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt
    67981 gtaacctgta gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata
    68041 gagatcgtga gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga
    68101 atggggccgg ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt
    68161 tcggtgaaag atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt
    68221 aaaccgggtg atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg
    68281 attgccgcgc tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa
    68341 cgccgtgcgg cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc
    68401 atggaaggtg cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc
    68461 gatcagttca ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc
    68521 tggcagcaaa ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact
    68581 atcaccggcg tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg
    68641 cagccggaag aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg
    68701 gggattttca acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa
    68761 aacaccatgc gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc
    68821 gcggttgccg gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc
    68881 atctgcgatc gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagt
    68941 cagggggcgg ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattaag
    69001 gcatgtaaaa aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc
    69061 ttcccggtaa tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg
    69121 cggttacggg tattgccgag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt
    69181 tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg
    69241 ttgatgcggg cttcttcatg aacagttaac ttcgcgtaag cggggagtgt cattagtagt
    69301 gtaatgacca gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc
    69361 gatttgactg ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag
    69421 gagaaaaaat taaatgagta gtttgttgtt gtttaacgat aagagtaggg cgctgcaggc
    69481 ggatatcgtc gccgtgcagt cgcaggtagt gtacggcagc gtaggcaaca gcattgcggt
    69541 gcctgctata aaacagaacg gcttgaatgt ctttgccgtg ccgacggtat tgctgagcaa
    69601 tacgccgcat tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta
    69661 tttgcgtgcg cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta
    69721 tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga
    69781 ccatcctgac ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta
    69841 tgtcaaacct gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcaaggaat
    69901 tacccccaat atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag
    69961 tgccattgct gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag
    70021 cgcctccggt aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt
    70081 gaatgtcatt tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg
    70141 tgctcagctt atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc
    70201 ggggttgcgc gtactggaag tgatgcgcta tacccagcag catgagagcg atgaattgat
    70261 tttgccgccg ctggcggaag cataaaaaat ggcgccgatg g
//
LOCUS       DAEEBB010000005        63143 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEEBB010000005 DAEEBB010000000
VERSION     DAEEBB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.005"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..413)
                     /locus_tag="KLF07_000306"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="KLF07_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="KLF07_000307"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="KLF07_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBD9830435.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="KLF07_000308"
     CDS             3156..3290
                     /locus_tag="KLF07_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830436.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="KLF07_000309"
     CDS             3367..3720
                     /locus_tag="KLF07_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBD9830437.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="KLF07_000310"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="KLF07_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="KLF07_000311"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="KLF07_000311"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBD9830438.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="KLF07_000312"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="KLF07_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBD9830439.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="KLF07_000313"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="KLF07_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBD9830440.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="KLF07_000314"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="KLF07_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBD9830441.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="KLF07_000315"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="KLF07_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD9830442.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="KLF07_000316"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="KLF07_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBD9830443.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /gene="hycB"
                     /locus_tag="KLF07_000317"
     CDS             10323..10934
                     /gene="hycB"
                     /locus_tag="KLF07_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBD9830444.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="KLF07_000318"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="KLF07_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD9830445.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="KLF07_000319"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="KLF07_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBD9830446.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /gene="hycE"
                     /locus_tag="KLF07_000320"
     CDS             13701..15398
                     /gene="hycE"
                     /locus_tag="KLF07_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBD9830447.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="KLF07_000321"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="KLF07_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBD9830448.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="KLF07_000322"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="KLF07_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBD9830449.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /gene="hycH"
                     /locus_tag="KLF07_000323"
     CDS             16714..17124
                     /gene="hycH"
                     /locus_tag="KLF07_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBD9830450.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="KLF07_000324"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="KLF07_000324"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBD9830451.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="KLF07_000325"
     CDS             17612..18385
                     /locus_tag="KLF07_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830452.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="KLF07_000326"
     CDS             18547..18858
                     /locus_tag="KLF07_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBD9830453.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="KLF07_000327"
     CDS             18855..19274
                     /locus_tag="KLF07_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD9830454.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="KLF07_000328"
     CDS             complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="KLF07_000328"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBD9830455.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="KLF07_000329"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="KLF07_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBD9830456.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /gene="ascG"
                     /locus_tag="KLF07_000330"
     CDS             22538..23548
                     /gene="ascG"
                     /locus_tag="KLF07_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBD9830457.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="KLF07_000331"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="KLF07_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBD9830458.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24376..26628
                     /gene="hypF"
                     /locus_tag="KLF07_000332"
     CDS             24376..26628
                     /gene="hypF"
                     /locus_tag="KLF07_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBD9830459.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26756..27889)
                     /gene="norW"
                     /locus_tag="KLF07_000333"
     CDS             complement(26756..27889)
                     /gene="norW"
                     /locus_tag="KLF07_000333"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBD9830460.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27886..29325)
                     /gene="norV"
                     /locus_tag="KLF07_000334"
     CDS             complement(27886..29325)
                     /gene="norV"
                     /locus_tag="KLF07_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBD9830461.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29512..31026
                     /gene="norR"
                     /locus_tag="KLF07_000335"
     CDS             29512..31026
                     /gene="norR"
                     /locus_tag="KLF07_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBD9830462.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="KLF07_000336"
     CDS             complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="KLF07_000336"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBD9830463.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="KLF07_000337"
                     /gene_synonym="gutR"
     CDS             complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="KLF07_000337"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBD9830464.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="KLF07_000338"
     CDS             complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="KLF07_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBD9830465.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="KLF07_000339"
                     /pseudo
     CDS             complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="KLF07_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="KLF07_000340"
                     /gene_synonym="gutB"
     CDS             complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="KLF07_000340"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBD9830466.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="KLF07_000341"
                     /gene_synonym="gutE"
     CDS             complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="KLF07_000341"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBD9830467.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="KLF07_000342"
                     /gene_synonym="gutA"
     CDS             complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="KLF07_000342"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBD9830468.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36215..37300
                     /gene="mltB"
                     /locus_tag="KLF07_000343"
     CDS             36215..37300
                     /gene="mltB"
                     /locus_tag="KLF07_000343"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBD9830469.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37445..37942
                     /gene="pncC"
                     /locus_tag="KLF07_000344"
     CDS             37445..37942
                     /gene="pncC"
                     /locus_tag="KLF07_000344"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBD9830470.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38022..39083
                     /gene="recA"
                     /locus_tag="KLF07_000345"
     CDS             38022..39083
                     /gene="recA"
                     /locus_tag="KLF07_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBD9830471.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39311..39811
                     /gene="recX"
                     /locus_tag="KLF07_000346"
     CDS             39311..39811
                     /gene="recX"
                     /locus_tag="KLF07_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBD9830472.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39939..42569
                     /gene="alaS"
                     /locus_tag="KLF07_000347"
     CDS             39939..42569
                     /gene="alaS"
                     /locus_tag="KLF07_000347"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBD9830473.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42804..42989
                     /gene="csrA"
                     /locus_tag="KLF07_000348"
     CDS             42804..42989
                     /gene="csrA"
                     /locus_tag="KLF07_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBD9830474.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43305..43397
                     /locus_tag="KLF07_000349"
     tRNA            43305..43397
                     /locus_tag="KLF07_000349"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43339..43341,aa:Ser,seq:gct)
     gene            43401..43477
                     /locus_tag="KLF07_000350"
     tRNA            43401..43477
                     /locus_tag="KLF07_000350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43435..43437,aa:Arg,seq:acg)
     gene            43541..43617
                     /locus_tag="KLF07_000351"
     tRNA            43541..43617
                     /locus_tag="KLF07_000351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43575..43577,aa:Arg,seq:acg)
     gene            43681..43757
                     /locus_tag="KLF07_000352"
     tRNA            43681..43757
                     /locus_tag="KLF07_000352"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43715..43717,aa:Arg,seq:acg)
     gene            43956..44032
                     /locus_tag="KLF07_000353"
     tRNA            43956..44032
                     /locus_tag="KLF07_000353"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43990..43992,aa:Arg,seq:acg)
     gene            44313..44879
                     /gene="yqaB"
                     /locus_tag="KLF07_000354"
     CDS             44313..44879
                     /gene="yqaB"
                     /locus_tag="KLF07_000354"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBD9830475.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44876..45304
                     /locus_tag="KLF07_000355"
     CDS             44876..45304
                     /locus_tag="KLF07_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD9830476.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45377..46933
                     /gene="gshA"
                     /locus_tag="KLF07_000356"
     CDS             45377..46933
                     /gene="gshA"
                     /locus_tag="KLF07_000356"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD9830477.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            47083..47598
                     /gene="luxS"
                     /locus_tag="KLF07_000357"
     CDS             47083..47598
                     /gene="luxS"
                     /locus_tag="KLF07_000357"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBD9830478.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47662..49200)
                     /gene="emrB"
                     /locus_tag="KLF07_000358"
     CDS             complement(47662..49200)
                     /gene="emrB"
                     /locus_tag="KLF07_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBD9830479.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49217..50389)
                     /gene="emrA"
                     /locus_tag="KLF07_000359"
     CDS             complement(49217..50389)
                     /gene="emrA"
                     /locus_tag="KLF07_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBD9830480.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50516..51046)
                     /gene="emrR"
                     /locus_tag="KLF07_000360"
     CDS             complement(50516..51046)
                     /gene="emrR"
                     /locus_tag="KLF07_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBD9830481.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(51137..51472)
                     /gene="ygaH"
                     /locus_tag="KLF07_000361"
     CDS             complement(51137..51472)
                     /gene="ygaH"
                     /locus_tag="KLF07_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBD9830482.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51462..52199)
                     /gene="ygaZ"
                     /locus_tag="KLF07_000362"
     CDS             complement(51462..52199)
                     /gene="ygaZ"
                     /locus_tag="KLF07_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBD9830483.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52323..53507)
                     /locus_tag="KLF07_000363"
     CDS             complement(52323..53507)
                     /locus_tag="KLF07_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9830484.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53798..54790)
                     /gene="proX"
                     /locus_tag="KLF07_000364"
     CDS             complement(53798..54790)
                     /gene="proX"
                     /locus_tag="KLF07_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBD9830485.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54848..55912)
                     /gene="proW"
                     /locus_tag="KLF07_000365"
     CDS             complement(54848..55912)
                     /gene="proW"
                     /locus_tag="KLF07_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBD9830486.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55905..57107)
                     /gene="proV"
                     /locus_tag="KLF07_000366"
     CDS             complement(55905..57107)
                     /gene="proV"
                     /locus_tag="KLF07_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBD9830487.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57463..58422)
                     /gene="nrdF"
                     /locus_tag="KLF07_000367"
     CDS             complement(57463..58422)
                     /gene="nrdF"
                     /locus_tag="KLF07_000367"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBD9830488.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58432..60576)
                     /gene="nrdE"
                     /locus_tag="KLF07_000368"
     CDS             complement(58432..60576)
                     /gene="nrdE"
                     /locus_tag="KLF07_000368"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBD9830489.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60549..60959)
                     /gene="nrdI"
                     /locus_tag="KLF07_000369"
                     /pseudo
     CDS             complement(60549..60959)
                     /gene="nrdI"
                     /locus_tag="KLF07_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60956..61201)
                     /gene="nrdH"
                     /locus_tag="KLF07_000370"
     CDS             complement(60956..61201)
                     /gene="nrdH"
                     /locus_tag="KLF07_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBD9830490.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61449..61778)
                     /locus_tag="KLF07_000371"
     CDS             complement(61449..61778)
                     /locus_tag="KLF07_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBD9830491.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61930..62274
                     /locus_tag="KLF07_000372"
     CDS             61930..62274
                     /locus_tag="KLF07_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBD9830492.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62311..62760)
                     /gene="alaE"
                     /locus_tag="KLF07_000373"
     CDS             complement(62311..62760)
                     /gene="alaE"
                     /locus_tag="KLF07_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBD9830493.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14864 a        16279 c        17013 g        14987 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gctcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cgactacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcatttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagactg ccggatacga cgcagtcgtg tccggcagga cgggttacgc aattcatgat
    24361 ggcgggaata actcaatggc aaaaaacaca tcttgcggtg tccaactgcg tattcgtggc
    24421 aaagtgcagg gcgtcggttt tcgtccgttt gtctggcagc tggcacagca attaaatctt
    24481 cacggcgatg tctgtaatga cggcgatggc gtagaagtcc ggctgcggga agacccggaa
    24541 acgttacttg ttcaattgca tcagcactgc ccgccgctgg cgcgtattga tagcgtcgag
    24601 cgtgagccgt acatctggtc acaactgccc actgagttca ccatccgcca gagcgcggga
    24661 ggtgccatga atacgcaaat tgtcccggat gccgctactt gccctgcttg ccttgccgaa
    24721 atgaataccc caggcgaacg gcgttatcgt tatccgttta tcaactgtac ccactgcggc
    24781 ccgcgtttca ccattattcg cgccatgcct tacgaccgcc cgtttaccgt gatggcggcg
    24841 tttccgttgt gtccggcttg tgacaaagag taccgtgacc cgctcgatcg tcgcttccac
    24901 gcccagccgg tggcctgccc ggagtgtggc ccacatcttg aatgggtaag tcatggtgaa
    24961 catgcagaac aagaggcggc attacaggcg gctatcgcac agttaaaaat gggcaacatt
    25021 gtcgccatca aagggattgg cggatttcat cttgcctgcg atgcacgtaa cagtaacgcg
    25081 gtggcgacac ttcgggcgcg caaacatcgc ccggcgaaac cgctggcggt catgttgcca
    25141 gtggctgacg gtttaccaga cgctgcgcgc cagttgctta ccacgcccgc cgcgccgatt
    25201 gtgctggtgg ataaaaaata cgttcctgag ctttgtgatg atatcgcccc tgaccttaac
    25261 gaagtcgggg taatgttgcc tgcgaacccg ctccagcatt tgctgttaca ggaactgcaa
    25321 tgcccgctgg tgatgacctc cggcaacctg agcggtaaac caccagctat cagcaacgaa
    25381 caggcgctgg cggatttgca gggcattgcc gacggattct tgatacataa ccgcgacatc
    25441 gtgcagcgga tggatgattc ggtggtgcgc gaaagcggcg aaatgctgcg ccgttcgcgg
    25501 gggtatgtgc cggatgcgct ggctttgcct ccgggcttta aaaatgttcc gcctgtgctg
    25561 tgtctcggcg cggatctgaa aaataccttc tgcctggtgc gcggtgaaca agcggtgttg
    25621 agtcagcatc tgggcgattt aagtgacgat ggcatccaga tgcagtggcg cgaagcgtta
    25681 cgcctgatgc aaaacatcta cgattttacc ccgcaatacg ttgtgcatga cgcgcatccg
    25741 ggctatgtct ccagccagtg ggcgcgtgaa atgaatctgc cgacgcaaac ggtactgcat
    25801 catcatgccc atgcagcggc gtgtctggca gagcatcagt ggccgttgga tggcggtgat
    25861 gtcattgctt tgacgctcga cggtatcggt atgggggaga acggcgcttt gtggggcggc
    25921 gagtgcctgc gggtgaacta tcgcgaatgt gagcacctgg gcggcttgcc tgcagtggcg
    25981 cttccgggtg gcgatttggc agcgaagcag ccgtggcgaa acctgctggc gcagtgcctg
    26041 cgctttgtgc cggagtggca gaattaccct gaaacagcaa gtgtgcaaca gcaaaactgg
    26101 agcgtgctgg cgcgggccat tgagcgtgga attaacgcgc cgctggcgtc atcgtgtggg
    26161 cggttgttcg atgcagtggc ggcggcactg ggctgtgcgc cagccacgtt aagttatgaa
    26221 ggtgaagcgg cttgtgctct ggaggcgctc gcagcctcat gccacggagt gacgcatccg
    26281 gtgacgatgc cgctggtgga caatcaactg gatctcgccg ctttctggca gcagtggctg
    26341 aactggcagg caccggttaa tcaacgcgcg tgggcgtttc atgatgcgct ggcgcagggt
    26401 tttgccgcgt tgatgcgtga gcaggccacg atgcgtggta tcactacgct ggtatttagc
    26461 ggcggggtta ttcataaccg tttgctgcgt gcacgtctgg cgcattatct cgctgatttc
    26521 acattgctct ttccacagag tttaccggcg ggtgatggcg gtttgtctct ggggcagggg
    26581 gttattgctg cggcgcgttg gttagcgggt gaagtccaga acggataaaa cgtgttgcat
    26641 ttggacacca ttgccggatg cggcgtaaac gccttatctg gcctacacat gcgtccccgt
    26701 aggtcggata agacgcgcca gcgtcgcatc cgacatttta ggcacagtag cccacctaca
    26761 tcggcaatgt tttcaacaat ccaaatgcct ctttcatccg atcctcactg accacaaagg
    26821 cgcgaagctg gtcagcatcg tcaacaccgc gagccaccat tccctggcgt tcggtattaa
    26881 tttgccagcg taaatcctga cgctgggttt cgcctgccag atgcagcggt aattccggcg
    26941 ttttgatttt caccagcatc gccggtagtt tcagcggcgt gttattgccg agaagatttt
    27001 ttgccagcac catcgcgcta agttgaatcg gctgcaggaa tggcagtacc tgaccgttga
    27061 tttccgcgca atcgcccagc gcataaatat cgtcattact ggtttgcaga taactatcga
    27121 cgcaaacgcc gcgattaatc gtcagcccgg cgcgtcgtgc cagggctgtt tccgggcgca
    27181 gtccggtggc ggcaattacc gcatccactt cgatactacg ctggtggtcc agcgttgcca
    27241 gaatgccaga atccgttttt tccagcccct gcaactgaga tttcaacagc agatgaacgc
    27301 ccatctccgt caaccgatgc tgcaagcggc tgcttacttc tggtggcatt aatgacgcca
    27361 gaatactggc agcgttgtcg attagcgtga ccgctttgcc tgcacgacaa aaatccatcg
    27421 ccagttcgct accaatcaaa ccaccgccga caatcaacac gcgtcgggca tcccgcagtt
    27481 gcgtttcaca ggcgcgatac tcttgctgac tatttaacgt cagcattaac tcacgcccag
    27541 gcactggcgg gacaaaggca ctggcaccag ttgccagcac cagcttgtcg tattgccact
    27601 gattattctg gcttttcacc acatgggctt cggcatcgat atccgtcacc caggtgtgcg
    27661 gaaacaggtg cagattaaac tgcccggcaa attcacccgc cgtctggcgg gtaaggtcgt
    27721 cggcacgttg cccctggctg ataacatggc tgaggtccgg tttgttgtac tcatccatgc
    27781 tgtcggcggc gatcagggtt aatggaatac tggcgtcctg tttgcgaata tttttcacca
    27841 gttggcgggc ggcgaagccc gaaccaatga tcacaatgcc gttgctcatt ttgcctccga
    27901 tgccagttcg tcaaagacgt ctttgccgag agagcattcc gggcagagga agttatccgg
    27961 aacttcactc cacggcgttc ctggcgcaac gtcctgcatt ggctcgcctt ttgccggatc
    28021 gtaaatccac tggcagacgc tgcactgcat ccgtgggccg aggtcggact tcgtggtggc
    28081 agaggtttct tctttaacca ccgtattcac cgtgctctgc ggcagcggcg cgagcgccca
    28141 ctgacgggcg atttcgcgac catgttcacg gcataactcc agagcgtcct ggtctggtcg
    28201 ccatttggct ttcaggctaa gcgacatttc gaaacccgca tcctgcaggc gcgtggaaag
    28261 acgatccacc gcaccgccgc tccagccgtg agagccgaaa gcactggcgc gtttgttacg
    28321 gaagcgtaat ccggtcatct cctccaccag accggcgatt ttcggcatca tcacgttatt
    28381 catcgtcgaa gtgccgacca gcacgccttt tgagcggaag acattggtca ggatttcgtt
    28441 tttatcgctt cgggcgacgt tgaaaatttt caccgccacg cgtgggtcgg tttccgcaat
    28501 cccctgggcg atagcgtcag ccatcatgcg ggtgttattc gacatggtgt cgtagaaaat
    28561 ggtgattctg tcttcctgat aatcagccgc ccatttcagg tacagctcga caatttgcgt
    28621 cgggttatcg cgccatacca cgccgtgaga ggtcgctatc atatcgactg gtaagttaaa
    28681 gcccaggatc tcggtaattt tcggtgttac caggcggctg aacggcgtca ggatattggc
    28741 gtagtaacgc tggcactgct cgaaaagctc cgtctgatcc acttcatcgt tgaacagatg
    28801 ctcgtcgcag tagtgctgac cgaaagcatc gttactgaac agcaccgcgt cgcctgtcag
    28861 gtaagtcatc atgctgtccg gccagtgcag cattggcgtt tcgacaaaaa tgagctgttt
    28921 gccgttgccg atatccagcg tgtcaccagt tttcaccaca ttaaaattcc actccggatg
    28981 atgatgatga ccatttatcg agtcgatagc gttggcagta cagtaaatcg gcgtatcggg
    29041 aatttgcgcc atcagttcgg tcagcgcccc agcgtggtcc tcttctgcat ggttaatcac
    29101 gatgtaatcg atatccgcca gatcgatttc attacgcagg ttctgcacaa attcgcggct
    29161 gaatttatgg tcgacggtgt cgatcagcac gtttttttct tcgcggatga ggtagctgtt
    29221 gtaactgctg ccgcgtagcg ttttatattc cgtaccgtga aaatcacgca cttcccagtc
    29281 acgttgacca acccaatgaa tgttattttt caccacaata gacatagcaa cctcaattta
    29341 ttcagcgtgt tctaaaaaga tgtcttgcta attgcagatt acgtgccagt tttttatctg
    29401 attgttttta ctctataaat aaaattatgc tcattaatga gtagtcaaaa tgactatcaa
    29461 tcaaatggtc gatatgacaa tatctatagt caaattgaca gtgaggcaaa gatgagtttt
    29521 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcatcaggat
    29581 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
    29641 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
    29701 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
    29761 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
    29821 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
    29881 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
    29941 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
    30001 gaacaactgg aaagccagaa tatgatgcca ggcgatgcca cgccgtttga agcggtgaaa
    30061 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
    30121 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga cgggaaccgg taaggagctg
    30181 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
    30241 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
    30301 tttactggcg ctatcagtaa ccgcagcggg aagtttgaaa tggcggataa cggcactctg
    30361 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
    30421 cagtatggcg atattcagcg cgttggcgat gaccgtagtt tgcgggtcga tgtgcgcgtg
    30481 ctggcggcga ctaaccgcga cttacgcgaa gaggtgatgg cagggcgatt tcgcgctgac
    30541 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
    30601 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggct ggggctctcc
    30661 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actatcgttt tccggggaac
    30721 gtgcgcgaac tggaacatgc tattcatcgg gcggtagtgc tggcgagagc cacccgcaac
    30781 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgccg
    30841 ccagaagcgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
    30901 cagcgtgaaa ctattcgcca ggcactggca caaaatcatc ataactgggc tgcctgcgcg
    30961 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggaatgaag
    31021 gattaaataa tcccggcctg atagaaatcc tgcaggttaa ttgcgccggt gagtttgccg
    31081 ttttcatcca ccaccggtgc ggcagtgatt ttgcgcttca tcaggatctc tttggcgtcg
    31141 atggcgcgac tttgcgcctg taacgtggtg ccgcctgtcg tcatcgcttc attaactggc
    31201 gtggtgagtg cgccgccgcc taccagccag cgacgtaaat cgccgtcggt aaagacgcct
    31261 tgtacctgtt gttgagcgtc acataccgcc accagcccca gaccggtgcg gctgagttcc
    31321 agcatcgcat ccatcacgct ggcggttaac gccacctgtg ggatggcatc gtcacggcgc
    31381 atcagatgat gcactttatt cagcaagcga gcgcccagtg ccccggctgg gtgggagcgg
    31441 gcaaaatctt cttcattaaa tccgcgcgcc tgcatgaccg ccatcgccag cgcgtcaccc
    31501 atcatcaggg tattgacggt gctggaggtc ggcgcaaggt gcatcgggca ggcttcgcgt
    31561 tctacggaga tatccagcac cgcttttgcc gccaggccca gcggtgacgt cggtttgccg
    31621 gtcatcgcca gcagcgcgat agatttatct tccagacgcg gaataatcag atccagttcc
    31681 ttcgcgccac cggagtaaga gataaacagc atcacatcgc ggctttcaat catccccaga
    31741 tcgccgtgca gtgcttctgc cggatggaca aaaaaagccg atgtgccggt actggcaagt
    31801 gttgcggcga ttttcttacc aatgtggccc gatttgccaa ttcccgaaac caccactttg
    31861 ccttcgcagt gcaggatgat attggcggcg cgaacaaaat catcgcccag acgttccggt
    31921 aaacggcttg cttcctgcaa ctccagcatt aacgtctgac gtcccgcgtt cagtagtgct
    31981 tcactcattg ctctctccgg ttatgatcac atcgatccct ttctcttcca gcgcctgacg
    32041 aaacgccgga tcgatacctg cgtcggtaat cagcttatcg acgctttcaa gactgcaaac
    32101 tacgttgggg cttttacggc caaactttga tgagtccgcc atcaaaatca cttcgcgcgc
    32161 ggcattgcac attgccttac tgacggtata aacctcgtta aaggtggtta cgcccgcatt
    32221 gagatcgatg ccgtcggtgc ccataaacaa tttatcgaaa ctgaaatgtt cgaaggcatt
    32281 ctctgccagc tgtccgtgaa atgaggccga ttttttacga aacgttccgc ctggcatcag
    32341 gatagtttgt tcgttatcca gttcagatag cgcattgacg atatgcagac tgttggtcat
    32401 caccgtgatg ttattaaagc gcgagagcag gggaaccatc tgcaaaacgg tactgccagc
    32461 atcaagaatg atcgaatcgc catcatgaat aaaactaacg gcagcttctg ctatcagctc
    32521 tttcttgtgg gtgttgatga gtgttttatg atcgataggc ggatcggatt cctctttatt
    32581 caacaccact ccgccataag tacgaatgac ggttcctgca tgttccagaa tgaccagatc
    32641 tttgcgaatg gttgtgcctg tggtgtcaaa gtattgcgcc aattcttcaa ccgagcattt
    32701 accctgcttt tgcagatact ccagaatggc ggcctgacgc tgacgaggtt tcatgggcgt
    32761 gattacctga tttaggtttc aaaatgataa cttcgcaagc aagtaacatt cacaacgaaa
    32821 ttatccacgt ttcagtttaa gcgccaatga tagagcattc tgtgatagtg gatcatgggg
    32881 aaagatcaca tcgggctgta aatcacccgc atgcataccg gtaattgcgg gaattttttg
    32941 cggtcgtgcg aagacggtca gtcctttcat aaacaaggtg tcgacgatgc gctgctgatc
    33001 gtccagggcg atggcgacca cgacccgtgg tttaaagcgc ccgctggaac ggcccacgcc
    33061 aacccgccct tgctggcata gtgtgtcgaa ggcacggtta aaacgagaaa tttgccagcc
    33121 gcctaaggcc agttgcgcac accaggcgat aacggcgacg gtgatgagtg cggataccat
    33181 aacttttcct taatgtagtg gcggaagtcc tgtatgcctg atgagcgtag cgcatcaggc
    33241 attctattag tctcacggag atccttaacg gatctccgct gttgattaca acatcacctg
    33301 accgccggtg acattgatcg actgtccggt gcagtacgac gccttaggac tggcatagag
    33361 cagcagcata ttcagcacat cctgataatc gcagccgcgt ttgagcggta ctttgtcgat
    33421 gtaatactgc tcgacttgat ccggtttgat acccagcttg gtcgcgtatt ggggtaacag
    33481 tgactggaac ataggcgatt tcagcaggtt accgagcatc agtgaatgca ccgtaatgcc
    33541 gtactccgcc agatccagcg ccagtgattg agtcagcccg acgccaccaa atttcgctgc
    33601 gctgtagcca gagttgtgtt tgctgcccac tttgccggat ttcgagttga tctgaataat
    33661 gcgaccctga atcccgtcgc ggatcatcaa acgagaaaat tcacgggcac acaggaaata
    33721 acccaccaga ttcacggtca aaatcgccga gctggaagtc gctgataaag gctgcttttg
    33781 ctattccggc gctgtagacc agcaaatcca cgcgcccaaa gatttcatct accccacgag
    33841 agagcgccag gacgctttgc tcgctggtgg cgtcagcacc aaaactatac gctgttcctt
    33901 caccatattc ggcgttaatt tcttgtgcca catttgcggc tttgtcgctc tgaatatcga
    33961 caaccgcgac gcgatacccc tcggcagcca gaccgtggca caggaacgcg cctaaggttt
    34021 gcccaccacc gatgacaacg gcaacctgat tcatttttta ctccttaaca gattcaaact
    34081 tcaaaaccga tcccggcgcg atatcgtcgg gtacagggcc tgccacatgg acagtgcccg
    34141 gaaattccgc ttcgtttaaa ccatcgaagc gcagggtgac atgacccagt tcgcgaaggt
    34201 tgtcttccgc cacgctgcca acagcggtca ccggatagcg atgctgcccg agcgtaaatt
    34261 gcaaaccggg atggagtgca cctttcaact cgccgtggca atgaatgaag caatactctt
    34321 cgaggtccgc tggcgcgcct tcacgaaagg tgatgagcat ctggtcgctg agggcgtcag
    34381 tggcactcgc gccgatacgg gtgatggtgg tctgataaat aacggtcatg tttcagcctc
    34441 tattgataga taaaaccgga gacaaaccag gcgatcagta cagtcggtgc gccggttaaa
    34501 aagtggctca ccagtacaga agggacaccg acgcgaaccg tgtcctgacg ggcttccgcc
    34561 agcgacaaac cgaccgggat gaagtcgcag gccgcctgcg cgttgatggc aaacagtgcc
    34621 ggtaaagcca gatgcggcgg aatattgccg agaccaatct gcacgccaat caatacgccg
    34681 ataacctgtg cgataactgc gcctgggccg aggaaaggtg aaagcagcgg gaaggagcag
    34741 atgagcgcca gcatgaccag acctagtgga tggctcgcca gcggagcaag accgtgggca
    34801 atccagtcac caaggccaga agccataatg atgccaatga gcgccgagac gaacgccata
    34861 aacggcagaa tggtttttaa tacagtgtcg atggtgtcac gaccagattg aaacaacacg
    34921 gcaacggcag aacccatacc cattcccacc ttcgccagta aaccatcgct ttgttcggtg
    34981 attttcttgc tggtgtcata gtcacggccc acggaagagg gttgtggtgt cgcatcaccc
    35041 actacagtga tgttttcttc ttttacgcca gagacataaa tatcttccac aatgtactgc
    35101 gccagcggac tggacttgcc cgtcgagtgg atattaatgg tgggaatacg tcgtttcgga
    35161 tagatgccgc agcgtaatgt gccgccacag tcgattaccg cgacaccaat ttccgcctcc
    35221 gcgggttcac cttctttaaa tccgtcaata gcctgccagc cagtaagttg tgccagtttg
    35281 tcaacaatcg caggccgggt accggcggtg atatagacga tttttttgcc cggcgtggct
    35341 tccagctcaa gcgggccgcc ccagccaccc gttccttttt cgatccgaat acgcgtcatg
    35401 atgttgctcc tgccaggtga actttttgtt caagttgaat gcccatcttt ttctcaaaaa
    35461 tggcggtggt cagatcggtc acccagccgc gaaagaagtt ggtgaccaga ccaaccagca
    35521 gatagctcac cgccagtggg ccaagtggca ggttcagcgt tgtcagaccg ctggcaatgc
    35581 caagataaac aaacagttcg ccagggttga tatggggaaa gaggccattc attgagtggc
    35641 agctataaga ggctgccgcg tagtagctgg gtttgtactt ttccggcata aagcgaccca
    35701 ggcttaaggt catcggattg caaaagacaa acgtgccaat gcacggtagc agcaggtaac
    35761 gggaaacagg gttaccggcg caacgttgag caaaacgttc aatacgatgc tgaccgataa
    35821 aattaatcag tgcgttcatg ataaccagca ggctaatcaa cagcggaaga atgccggtca
    35881 ccatcccggt aaacacctct ccgccctttt ggaacagccc gataaaccac tctgcaccat
    35941 gagtaatggt ttctatcatt gttctctcct tcaggattta ttgttttatt accaaacggc
    36001 aacctaatct aatcagattg aaagatttga aagtgttatt ttgatcgcaa aatgaaagat
    36061 aaatatttta atttgaaagt ttgaataaaa ggatagcgag gggaatgagt tgagttatgt
    36121 aaagtccgta tcgggcagtg actaccgctt ccttgtgcgg ggcgtgatgc tttaccatac
    36181 ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt atgtaacatt
    36241 gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta ctgagactga
    36301 aacgaccacc ggaacgccgt ctggcggctt cctgcttgag cctcagcaca atgtgatgca
    36361 gatgggcggc gatttcgcta ataacccgaa tgcccagcag ttcatcgaca gaatggtgaa
    36421 caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga agcgtctgga
    36481 ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac cgccatcagg
    36541 tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca acgtgcagaa
    36601 cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg
    36661 cgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg ggcgcgtgat
    36721 ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc cacgccgcgc
    36781 ggagtatttc tctggcgagc tggaaacctt cctgttgatg gcgcgcgacg agcaggacga
    36841 tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggacagt ttatgccgtc
    36901 gtcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaacc tgtgggatcc
    36961 ggttgatgcg atcggtagtg tggcgaacta tttcaaagcg cacggctggg tgaaaggcga
    37021 tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg ccaaatggct tcaaaaccag
    37081 gtacagcatt tcgcagcttg ccacggcagg tttaacgcca cagcagccgc tgggcaacca
    37141 tcaacaagcc agcctgctgc gtctggatgt cggtaccggt taccagtact ggtacggtct
    37201 gccgaacttc tacaccatta cccgttataa ccacagcacc cattacgcaa tggcggtctg
    37261 gcagttagga caagccgtgg cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg
    37321 tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca
    37381 acaccatctt cctgacgaaa gtgctatctt gtccggcata aatttttgac tgactgaggt
    37441 tgtgatgact gacagtgaac tgatgcagtt aagcgaacag gttgggcagg cgctgaaagc
    37501 ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat
    37561 taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct acagtaacga
    37621 agccaaagcg cagatgatcg gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag
    37681 tgaacccgtc gtggtggaaa tggcgatagg tgcactgaaa gcggctcgtg ctgattatgc
    37741 cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc ctgtcggcac
    37801 cgtctggttt gcttttgcca ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag
    37861 cggcgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca
    37921 acaatttcta caaaacactt gatactgtat gagtatacag tataattgct tcaacagaac
    37981 atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc gacgaaaaca
    38041 aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt aaaggctcca
    38101 tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc ggttcgcttt
    38161 cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc gaaatctacg
    38221 gaccggaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca gcgcagcgtg
    38281 aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc tacgcacgta
    38341 aactgggcgt cgatatcgat aacctgctgt gctcccagcc ggacaccggc gagcaggcac
    38401 tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc gttgactccg
    38461 tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct cacatgggcc
    38521 ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg aagcagtcca
    38581 acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc
    38641 cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt ctcgacatcc
    38701 gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag
    38761 tggtgaagaa caaaatcgct gcaccgttta aacaggctga atttcagatc ctctacggcg
    38821 aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag ctgatcgaga
    38881 aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa
    38941 ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt
    39001 tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc gaaggcgtag
    39061 cagaaactaa cgaagatttt taatcgttcg ttattgatga taatagataa tgtgtgcacg
    39121 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc caacactggc
    39181 gtgaaaaatt actctcacct cgatcctcta acgaattggc tgcacactca ttgactatta
    39241 aatctgttta tcagcaaaac ctgggtcgca tctgcggccc ttttgctttt ttaagttgta
    39301 aggatatgcc atgacagaat caacatcccg tcgcccggca tatgctcgcc tgttggatcg
    39361 tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa gaactgcgac gtaaactcgc
    39421 ggcaccgatt atgggcaaaa atggcccaga agagattgat gctacggcag aagattacga
    39481 gcgcgttatt gcctggtgcc atgaacatgg ctatctcgat gacagccgat ttgttgcgcg
    39541 ctttatcgcc agccgtagcc gcaaaggtta tggacctgca cgtattcgtc aggaactgaa
    39601 tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg cgtgaatgtg acatcgactg
    39661 gtgcgcactg gtgcgcgatc aggcgacgcg aaaatatggt gaacctttgc caactgtctt
    39721 ttcagaaaaa gttaagatcc agcgttttct gctctatcgt ggctatctga tggaagatat
    39781 ccaggatatt tggctaaatt ttgccgactg aacgcatacg gtattttact tcccactcaa
    39841 gaaaacttat cttattccca cttttcagtt accagcccgg cggttaagac acgctggagc
    39901 tggtggcgat atttcgttag cttgatttca ggataattat gagcaagagc accgctgaga
    39961 tccgtcaggc gtttctcgac tttttccata gtaagggaca tcaggtagtt gccagcagct
    40021 ccctggtacc ccataacgac ccaactttgt tgtttaccaa cgccgggatg aaccagttca
    40081 aggatgtgtt ccttggactc gacaagcgta attattcccg cgctaccact tcccaacgct
    40141 gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaatgt cggttacacc gcgcgtcacc
    40201 ataccttctt cgaaatgctg ggcaacttca gcttcggcga ctatttcaaa cacgatgcca
    40261 ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt tgccctgccg aaagagcgtc
    40321 tgtgggttac cgtctatgaa agcgacgacg aagcctacga aatctgggaa aaagaagtag
    40381 ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa aggtgcgcca tacgcatctg
    40441 acaacttctg gcagatgggt gacactggtc cgtgcggccc gtgcaccgaa atcttctacg
    40501 atcacggcga ccacatttgg ggtggccctc cgggaagtcc ggaagaagac ggcgaccgct
    40561 acattgagat ctggaacatc gtcttcatgc agttcaaccg ccaggcagat ggcacgatgg
    40621 aaccgctgcc gaagccgtct gtagataccg gtatgggtct ggagcgtatt gctgcggtgc
    40681 tgcaacacgt taactctaac tatgacatcg acctgttccg cacgttgatc caggcggtag
    40741 cgaaagtcac tggcgcgacc gatctgagca ataaatcgct gcgcgtaatc gctgaccaca
    40801 ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc gtccaatgaa aaccgtggtt
    40861 atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg caatatgctc ggcgcgaaag
    40921 aaaccttctt ctacaaactg gttggtccgc tgatcgacgt tatgggctct gcgggtgaag
    40981 acctgaaacg ccagcaggcg caggttgagc aggtgctgaa gactgaagaa gagcagtttg
    41041 cccgtactct ggagcgcggt ctggcgttgc tggatgaaga gctggcaaaa ctttctggtg
    41101 atacgctgga tggtgaaacg gctttccgtc tgtacgacac ctatggcttc ccggttgacc
    41161 tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga cgaagctggt tttgaagcag
    41221 caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg ctttggtgcc gattacaacg
    41281 caatgatccg tgttgatagt gcatctgaat ttaaaggcta tgaccatctg gaactgaacg
    41341 gcaaagtgac cgcgctgttt gttgatggta aagcggttga tgccatcaat gcaggccagg
    41401 aagctgtggt cgtgctggat caaacgccat tctatgcgga atccggcggt caggttggtg
    41461 ataaaggcga actgaaaggc gctaacttct ccttcgcggt ggaagatacg cagaaatacg
    41521 gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc tctgaaagtg ggcgacgctg
    41581 tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg tctgaatcac tccgcaacgc
    41641 acctgatgca cgctgcgctg cgccaggttc tggggactca tgtatcgcag aaaggttcac
    41701 tggttaacga caaggtgctg cgcttcgact tctcacacaa cgaagcgatg aaaccggaag
    41761 agattcgtgc ggtcgaagac ctggtgaacg cacagattcg tcgcaatttg ccgatcgaaa
    41821 ccaacatcat ggatctcgaa gcggcgaaag cgaaaggtgc gatggcgctg ttcggcgaga
    41881 agtatgatga gcgtgtacgc gtgctgagca tgggcgattt ctccaccgag ctgtgtggcg
    41941 gtactcacgc cagccgcact ggtgatattg gtctgttccg catcatctct gaatcgggta
    42001 ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga aggcgctatc accaccgttc
    42061 atgcagacag cgatcgctta agcgaagtcg cgcatctgct gaaaggcgat agcaataatc
    42121 tggcggataa agtgcgctca gtactggaac gtacgcgtca gttagaaaaa gaactacaac
    42181 agcttaaaga acaagctgcc gcacaggaga gcgcaaatct ttccagtaag gcaattgatg
    42241 ttaatggtgt taagctgttg gttagcgagc ttagcggtgt tgagccgaaa atgttgcgta
    42301 ccatggttga cgatttaaaa aatcagctgg ggtcgacaat tatcgtgctg gcaacggtag
    42361 ccgaaggtaa ggtttctctg attgcaggcg tatctaagga cgtcacagat cgtgtgaaag
    42421 caggggaact gattggtatg gtcgctcagc aggtgggcgg caagggtggt ggacgtcctg
    42481 acatggcgca agccggtggt acggatgctg cggccttacc tgcagcgtta gccagtgtga
    42541 aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca ggcaatgccg tggactcgct
    42601 tcacggcatt cgcattaacg ctatcgacaa cgataaagtc aggttgaagt tgtgtatatc
    42661 ggctaaactt aggtttaaca gaatgtaatg ccatgactgc ttacatgtaa tgtgtttgtc
    42721 attgcttact ttttggcgtt atatgatgga taatgccggg atacagagag acccgactct
    42781 tttaatcttt caaggagcaa agaatgctga ttctgactcg tcgagttggt gagaccctca
    42841 tgattgggga tgaggtcacc gtgacagttt taggggtaaa gggcaaccag gtacgtattg
    42901 gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga gatctaccag cgtatccagg
    42961 ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt ctcatcttta tcggtgagac
    43021 gcaccctcaa aatttctcct tcactctata gtcttttcgc tttactcccg ttcattcaac
    43081 ttaagtctcc atttttttgc attactgcta tctgccagac ctccgttttt ctgtagataa
    43141 aacactcttt ttgacgtttt tacagactaa ttgagcgtga agtgtgcaaa cgataaaagt
    43201 gtgggaaaaa ttgtttgact tataagtctc agaaagtaat atgtgcgcca cgcagcgacg
    43261 atgagcgata aacaagttct tcgaagcact cgtaagaggc gtgtggtgag gtggccgaga
    43321 ggctgaaggc gctcccctgc taagggagta tgcggtcaaa agctgcatcc ggggttcgaa
    43381 tccccgcctc accgccattt gcatccgtag ctcagctgga tagagtactc ggctacgaac
    43441 cgagcggtcg gaggttcgaa tcctcccgga tgcaccatct cttacttgat atggctttag
    43501 tagcggtatc aatatcagca gtaaaataaa ttttcccgat gcatccgtag ctcagctgga
    43561 tagagtactc ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccacct
    43621 cttacttgat atggctttag tagcggtatc aatatcagca gtaaaataaa ttttcccgat
    43681 gcatccgtag ctcagctgga tagagtactc ggctacgaac cgagcggtcg gaggttcgaa
    43741 tcctcccgga tgcaccatat tctccgaaat cttcagcaat gaaggtatcg aagaggtagc
    43801 ggaattaacc gcaggcacca gggatgataa cgttgcttta gcaacggccc gaagggcgag
    43861 ccgcaaggcg agtaatcctc ccggatgcac catctcttaa ttgatatggc tttagtagcg
    43921 gtatcaatat cagcagtaga ataagttttc ccgatgcatc cgtagctcag ctggatagag
    43981 tactcggcta cgaaccgagc ggtcggaggt tcgaatcctc ccggatgcac catattctcc
    44041 gtaaccttca gcaatgaagg tatcgaagag atagcggtat taaccgcagg caccaggaag
    44101 gataacgttg cgccagcaac ggcccgaagg gcgaacgtca gtgagtcatc ctcccggatg
    44161 caccatcttc tccaaaaaat ttccttcaaa aatatctcat ttctaaataa ctcctttatc
    44221 catttcctga attttcttct ccttcgccat cagcgacaaa atcaagcaat tacgctaaag
    44281 tagcgtcata tttttcgact tgcgagaaga ccatgtacga gcgttatgca ggtttaattt
    44341 ttgatatgga tggcacaatc ctggatacgg agcctacgca ccgtaaagcg tggcgcgaag
    44401 tattagggca ctacggtctt cagtacgata ttcaggcgat gattgcgctt aatggatcgc
    44461 ccacctggcg tattgctcag gcaattattg agctgaatca ggccgatctc gacccgcatg
    44521 cgttagcgcg tgaaaaaaca gaagcagtaa gaagtatgct gctggatagc gtcgaaccgc
    44581 ttcctcttgt tgatgtggtg aaaagttggc atggtcgtcg cccaatggct gtaggaacgg
    44641 ggagtgaaag cgccatcgct gaggcattgc tggcgcacct gggattacgc cattattttg
    44701 acgccgtcgt cgctgccgat cacgtcaaac accataaacc cgcgccagac acatttttgt
    44761 tgtgcgcgca gcgtatgggc gtgcaaccga cgcagtgtgt ggtctttgaa gatgccgatt
    44821 tcggtattca ggcggcccgt gccgcaggta tggacgctgt ggacgttcgc ttgctgtgag
    44881 tgaagcgtta tcgcttttct cgttgtttgc cagtagtttt ctcagcgcta cactattacc
    44941 cggcaactcc gaagtcgtgc tggtggcaat gttgctttcc gggatcagtc atccctgggt
    45001 tttagtttta acagcaacaa tgggtaatag ccttggaggg ttaactaacg ttatccttgg
    45061 gcgtttcttt ccattgcgta aaacatcgcg ctggcaagag aaagctatcg gctggctgaa
    45121 acgctatggt gcagtcacgc tattattaag ctggatgccc gtggttggcg atttactgtg
    45181 tctgttagcg ggatggatgc gcatctcgtg gggaccggta atcttttttt tgtgccttgg
    45241 taaagcgtta cgctatgttg cagttgcagc agcgaccgtt cagggcatga tgtggtggca
    45301 ctaattgtag gcctgcacat atggtcacca ttacagttat gctaattaaa acgattttga
    45361 caggcgggag gtcaatttga tcccggacgt atcacaggcg ctggcctggc tggaaaaaca
    45421 tcctcaggcg ttaaagggga tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc
    45481 tgatggcaca ctggcaacaa caggtcatcc tgaagcatta ggttccgcac tgacgcacaa
    45541 atggattact accgattttg cggaagcatt gctggaattc attacaccag tggatggtga
    45601 tattgaacat atgctgacct ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg
    45661 cgatgagcgg atgtggccgt taagtatgcc atgctacatc gcagaaggtc aggacatcga
    45721 actggcacag tacggcactt ctaacaccgg acgctttaaa acgctgtatc gtgaagggct
    45781 gaaaaatcgc tacggcgcgc tgatgcaaac catttccggc gtgcactaca atttctcttt
    45841 gccaatggca ttctggcaag cgaagtgcgg tgatatctcg ggcgctgatg ccaaagagaa
    45901 aatttctgcg ggctatttcc gcgttatccg caattactat cgtttcggtt gggtcattcc
    45961 ttatctgttt ggtgcatctc cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc
    46021 gctgccgttt gagaaaaccg agtgcggtat gtattacctg ccgtatgcga cctctcttcg
    46081 tttgagcgat ctcggctata ccaataaatc gcaaagcaat cttggtatta ccttcaacga
    46141 tctttacgag tacgtagcgg gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc
    46201 gaagattggt attgagaaag acggtaagag gctgcaaatc aatagcaacg tgctgcagat
    46261 tgaaaacgaa ctgtatgcgc cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc
    46321 ttctgatgcg ctgttacgtg gcggcattga atatattgaa gtgcgttcgc tggacatcaa
    46381 cccgttctcg ccgattggtg tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt
    46441 ctggtgtgcg ctggctgatg caccggaaat gagcagtagc gaacttgcct gtacgcgcgt
    46501 taactggaac cgggtgatcc ttgaaggtcg caaaccgggc ctgacgctgg gtatcggctg
    46561 cgaaaccgcg cagttcccgt tactgcaggt gggtaaagat ctgttccgcg atctgaaacg
    46621 cgtcgcgcaa acgctggata gcattaacgg cggcgacgcg tatcagaaag tgtgtgatga
    46681 actggttgcc tgcttcgata atcccgatct gactttctct gcccgtatct taaggtctat
    46741 gattgatact ggtattggcg gaacaggcaa agcgtttgca gaagcctacc gtaatctgct
    46801 gcgtgaagag ccgctggaaa ttctgcgcga agaggatttt gtagccgagc gcgaggcatc
    46861 tgaacgccgt cagcaggaaa tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga
    46921 aaaacacacc tgacagaaaa gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga
    46981 attcagggat gatgataaca aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa
    47041 agagttcaga aaatttttaa aaaaattacc ggaggtggct aaatgccgtt gttagatagc
    47101 ttcacagtcg atcatacccg gatggaagcg cctgcagttc gggtggcgaa aacaatgaac
    47161 accccgcatg gcgacgcaat caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa
    47221 gtgatgccag aaagagggat ccataccctg gagcacctgt ttgctggttt tatgcgtaac
    47281 catcttaacg gtaatggtgt agagattatc gatatctcgc caatgggctg ccgcaccggt
    47341 ttttatatga gtctgattgg tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg
    47401 gcaatggaag acgtgctgaa agtgcaggat cagaatcaga tcccggaact gaacgtctac
    47461 cagtgtggca cttaccagat gcactcgttg caggaagcgc aggatattgc gcgtagcatt
    47521 ctggaacgtg acgtacgcat caacagcaac gaagaactgg cactgccgaa agagaagttg
    47581 caggaactgc acatctagtc agtaaactat cttcacaatt aattgaaaaa agccagttca
    47641 aatgaactgg cttagttgta cttagtgcgc accgccgcca ccgccacctg cgccaaatgg
    47701 cggtttagca aaccacacca gccccagcag tacgaggaat atcccggctg acatccagaa
    47761 gatctcattg gcggaaataa tcagcccctg attggtgatc tgctgggcaa tccagcctga
    47821 tgcctgctgt tgcgtcatcc caagcccttc cagttgactg tacatcgcct gggcattcgg
    47881 gttgaacggg tttaccgact cagtcaactg cgcatggtgc atcgactcgc ggttggtcca
    47941 catggtcgtg gttatcgacg tgccgataga ccccgccagc gttcgcgtaa agttagagag
    48001 gctcgatgcc gccgccagtc gttccggtgg caaaccagac agcgtaatgg tggtcagcgg
    48061 cataaagaag caggccaccg caaacccctg gataaactgc ggccaggccg acgcgccaaa
    48121 atccatacct ggttcaaagg tataggcacg ccagtagaag cagacggcat acataataaa
    48181 gctgaaggtt accagccgcc gcatatccag tttatgcgcg aagcggccga taatcggcga
    48241 caggatcacc ggaataatcc ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta
    48301 accgtagacc tcctgcaaca actgcggcag cagaacaata gcgccgaagt agagcatata
    48361 cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca acgacagatc
    48421 gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag ccaccaccgc
    48481 caccacggta aggatgataa tttcctgtga tgaaaaccag tccagctctt taccgcggtc
    48541 gagcataatc tgcaggctgc cgataccaat aaccagcagt gccagcccca cggcatcaat
    48601 ccgccgccgt ttggtgcggg tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac
    48661 cgccacgcca atcggcacgt tgatgaagaa tatccagccc cagtggtaat tatcgctgat
    48721 ataaccgccg aggatcgggc cgcaaattgg cgcgacaatc accgtcatcg accacaacgc
    48781 cagcgcgatc gagcgtttgg ctggcgggta gttattcagc aatagacttt gcgaaagcgg
    48841 gatcaacggc ccggcgacaa tcccctgaat cacgcggaag aagatcagca tattcaggct
    48901 gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc aaaggaacag
    48961 tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga tggcattcgc
    49021 caccccgaaa gaagtgatta cccacgttcc ctggctgagc gatgagccca gattcccggc
    49081 gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga atgtcgccag
    49141 tgacagcgca atcgtcataa tgaccagttg cgcgccttcc agcggttttt gctgttgcat
    49201 cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc agtttattga
    49261 caggtgccag gctgatttca cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat
    49321 ttgccagtac ctgaccgtca cggttagtgg tattgacgct caccagtgtg gacaaaccaa
    49381 tgcgcagcgg gtattgctcc agctgtttct ggtccagttc gatacgcaca ggtagacgct
    49441 gaacgacttt gatccagtta ccggtcgcat tttgcgccgg aagcagtgag aatgcgctac
    49501 ctgtgcccat atccagacca accactttac cggtgtattt cacatcatcg ccgtaaatat
    49561 ccgtggtgat agtgaccggc tgaccgatac gcatattggc aatctgcgtc tctttaaagt
    49621 tggcatccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc gttgggctaa
    49681 tttgcgcccc aggctgtacc gcacggcggg agacataacc ggtcatcgga ctgacaatac
    49741 gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt tgcacggcag
    49801 gctgatcttc cagtttagtc cccagaatca tcgcctgatt ggcattgtat tgttgaatag
    49861 cgacgtccag ttgcgcctgg gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc
    49921 gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt tgtgcttttg
    49981 cgagggcgat tttctgcacc tcaatattcg cctgcaactg tttgctgtta atcatcagct
    50041 ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac gcctggcgag
    50101 catctgtcgg gtcgagagtg accagcacgt cgccttcttt tacaaaatcg gtgttatcgg
    50161 cccagacttt cgtcacgctg ccagacacct gagacataat ttgaatctga ttccctgcca
    50221 cgtatgcgtc atcggtttct tcgaaatgac gcagtaccaa aaaccaataa atccctatcg
    50281 ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta cgtttgccgc
    50341 tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata ttgttctcca
    50401 cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag catgttgcga
    50461 tgctggccgg tcattttttc ttttataaat ctggattttt gagcgagatg acgcgttagc
    50521 tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc gagacgggag agcaatttgc
    50581 gggtgatttg ctcgagctga tctttttctg ttgtgctgag cgcggaccag agttgatgca
    50641 ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt
    50701 gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt
    50761 ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca caacttaatt
    50821 cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc ataaacaacg
    50881 tctcgttaat cccctgagcc ttcagcattt tattgcggtt ctccagcagc ttgctttgca
    50941 tgtgcatgca aagacgagtc agaaggatct cctgataagg aaaatcttcg tggcggctgg
    51001 cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg gtatgacctc
    51061 attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat tgttttagaa
    51121 aaatgattct tgtgggttat ataatcgcca tcactttcca ggcgagccca taggccagcg
    51181 cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca cccagtaccg
    51241 cgaagccgac cagcgtgggc acgaaacggc gtgtatcgtg catcacttct ggtgcggtag
    51301 agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa atacctaccg
    51361 cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaaa tagcggaagc
    51421 aataattcgc cgcgccaacc agtaacccag gcagcagaac ctcatagctc atcgggcgct
    51481 ccttgccaga atgcttggat taacgcagtg aggcagccac agacaatgcc tgccagaatg
    51541 gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc tgcggtaacg
    51601 caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag tgccggaagc
    51661 ataaaaccta atgctgcttc aacggcggga taaccttgca gcaagccgct gccagagaat
    51721 gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat gccgatcatc
    51781 cagttctcgc tccagcggcg attattgcgt accagttttg ctgttgcggc ggcaaaaacc
    51841 tcatccgtca ggccaaacgc ccacagggcg gttttcgatt tttgcagacg ctgaataata
    51901 cggctacgca gtgacgggcc atacaacaca tggcgaacat ccattgccat gacggtcagt
    51961 gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg
    52021 cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc
    52081 gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg taaactgtct
    52141 ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct gtggagtagg gctttccata
    52201 gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc ttaaccttat
    52261 cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg gttgctgatt
    52321 gcttaatttg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa gttaactagg
    52381 gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc catgttgcca ggctgaggct
    52441 gaaattagcg aaccggcggc accgccaata aagtagctgg tcatgtaacc tgcggtcagg
    52501 cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat atgcacgccc
    52561 tgtacggtga gatccagcac caggatgccg ataatcagcg ccagtacgga agtgtgacca
    52621 aaccagatcg ccagccatga aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc
    52681 gatttaccct tatcggcaaa accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc
    52741 gcaagtccaa acagaccaat gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa
    52801 aaggccattg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg
    52861 cgggtgcgga ggatcttatc gctgataaac atgctgaaaa cggaacctaa caactgtggg
    52921 tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc tagcgccatc
    52981 agtgccatta acaccgaggc aacccaaaag acggtgcgcc agccgccgag gtttgccagt
    53041 aacccggcaa cggttcgtgc cagcaagatc cccaacagca gaccgctcat aatggtgcca
    53101 accactttgc cgcgtttatc cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt
    53161 gccacgactg agaataaacc ggttaatgcc gtaccgagga tcatcatcgc cagcgactga
    53221 ctgctggcag tgattaacat gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg
    53281 cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgctgc atagcccaac
    53341 tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg
    53401 gtgtcgagca atggctgggc gtaatagtta ctggctaccg cctgaccggt ggcgatagac
    53461 atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat ttttgttcat
    53521 caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatggg ggcgaaagat
    53581 agcttgttga aatgtgcgat gtggtttgta ggcatgataa gacgcgccag cgtcgcatca
    53641 ggcattgtgc acagaacggc ggatgcggcg aaaacgcctt atccgccata cggaatgagt
    53701 gtattttgta ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaatggc
    53761 ggatgaggca aaaacgactt atccgcccga ataaaaatta cttctgcgct gccagcgcct
    53821 cattcaccca gccatcgaac tgctgctggt gggctttgat ccaaccatca acatggccct
    53881 gaatatcgcc ttctgaggct ttgccgtcat gcataatggc gttctgggcg ttaatatctg
    53941 ccactggcaa ctgcataatg gcaaaaagtt tcgctgctgc cgggtttttc tcggcccagg
    54001 ctttgttggc aacgatatgc atggtgctga ccgggaagcc ataattcgca ccattcggca
    54061 gtttggtatc ggcgttttta tcgcccggca gtgcggagaa cggcacctgc aaccagacca
    54121 catctttccc tggcttcagc tcattactca cccagtacgg cgtccaggtg taataaaaca
    54181 ccggtttgcc ctctttgtag cgactgatgg tgtcggccat catcgctgcg tagttcccct
    54241 gattatgcgt cacggtgttg gtcagttcat acgcggcaag ctggtggttg atcgcacctt
    54301 cgcagcccca gcctgggtta cagccggtta aatccgcttt accgtcgccg ttggtatcga
    54361 acagtttggc gatcttcgga tctttcagtt gtgcgatgtt ggtgattttg tactggtcgg
    54421 cggttttctt atcgatcagg taaccctgtg ccgcgccgtt aacgaatatc ccttcacaat
    54481 aaaatttctt atcgccaccg gcagcttcgt acatgttgtc atgcagcggc gtccagttca
    54541 cggcggtgaa ggttgcatcg ccggaagcaa gtgaggtgta gccaacgttg taatccactt
    54601 cgctgggttt attgacggta taacctaatt tctccagtgc acgactgacc agcagcgtct
    54661 ggaaggtttc ttcagtgatg gtgctctgaa ctggattaac agtaatgcct ttgcccggca
    54721 gatcggctgc aaaagtttgt gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc
    54781 tatgtcgcat tgttattcct ttttttggca gggtgataat gcccggcgtt accgggcaag
    54841 tgcagagtta cttaatgaat gggcgggtca gcagaccgac agggccagtg gtgtaccagc
    54901 gacggttgcc gcgactgcgt gagtcgcgcc caacggcctg cgtcaggcgg tcgaggataa
    54961 tggcgaggat cacaatcccg acgccgccaa cggtggcaag gcccatatcc agacgaccga
    55021 taccgcgaag taccatctga cccagcccgc cgacggcaat catcgaggcg atgaccacca
    55081 tagaaagagc cagcatcagc gtctggttaa cgcccgccat aatggtcggc atcgccagcg
    55141 gtaactgaac tttgaacagc atctggcgcg ggctggcacc gaatgagcgc gaggcttcaa
    55201 tcagatccgc cggaacctgg ttaatcccca gaatcgtcag acggataatc ggcggcagag
    55261 caaagatgat cgtcaccact acgcccggca cgttaccgat accaaacagc atgacgattg
    55321 gtaccagata aacaaacgct ggcgtggtct gcatggcatc aagcagtgga cgaataattt
    55381 tcgccgctcg cggacttctc gccagccata tccccaacgg caaaccgatg acgatacaga
    55441 acagcagggc ggttaacacc agcgccagag tcaccattgc ctgcgaccag gcaccgattg
    55501 cgccgatggc aatcagcgaa accagcgtcg ccacgcccat tccgaccccg gaaatctgcc
    55561 aggcgatgag agcgaaaacg ataatcgcca ctggcgctgg catacccagc agcaattgct
    55621 ggaaactgtt gaggatataa tcaaccggaa cgcgcacgcc ctggaagacg ggacggaaat
    55681 gggtaacaac ccagtcgatc ccttcagtga cccaactgtc gagcgggatc agcgttttat
    55741 ggaacggatc gagaatatta aaatgctcga cgtttggcgc aggcgtactg gtcagccagt
    55801 cagcaccacc gccgtcagtc ggtgcagtcg tcggtgtacc ccaggcgtct gcggattgcg
    55861 cggcactgtc cgccgctggc gtggtatccc acggattatt ttgatcagcc attatttacc
    55921 ccctcacgat ctaaagcgcg cagcagcatt cctttcgaaa tgatgccgac atactgttgg
    55981 tcctcgtcga ccacgggcac cgcacagggt gcctgtccga catgagagag caactcgcta
    56041 agaggcgttt gtgcatcgac tgctaacggc gcatcaatca gcgccgcatc aagaccttgc
    56101 tgctgcgtta acgcggtttt aagcgaatcg atggagactg cgccgacaaa cttattaccg
    56161 cgttcgataa cgtagccata ttcgcgatct tcatcctgca ataatttcag tgccgaacgt
    56221 gggccgaagc caggggtttt acgaattaag ccattcggtg tccggcgggc aatatctttc
    56281 gcactgaata cctgactaat atcaacgcca cggaagaagg tacggacata atcattcgcc
    56341 ggattattga gaatttcatc cggtgtgccg acctgtacca cttcaccatt ttgcataatg
    56401 gcaattcggt cgccaatacg catggcttca tcgagatcgt gggaaataaa gacaatggtg
    56461 cgctgatgtt tcgcctgtaa ttttaccagc tcatcctgca tctcggtgcg aattaatgga
    56521 tcgagcgccg agaaggcttc gtccattaat aaaatatccg gattaatcgc taacgcgcgg
    56581 gctaatccca cacgttgacg catcccgcca gagagttcat ccgggtagct gtgggcataa
    56641 ttttccagcc cgacctgacg cagtgcatca agggcttttt cccggcgttc ttcggcatta
    56701 attccggcca attccatacc gaacgcagta ttgtccagca cggtcatatg cggcattaag
    56761 gcaaaggact ggaagaccat cgcaatcttt tttctgcgca cctcacggag tttggcatca
    56821 gatattttgg caatatccac accatcaatc agcacttgcc cgcgggtggg ttcaatcagg
    56881 cgattgagaa ggcgtaccat tgtggattta cccgagccgg ataatcccat gatgacaaat
    56941 atctcgcctt cttcaatggc cagactggcg tcttttacgc caagcgatag cccagttttt
    57001 tccagaattt gttcttttga aagtccttgt tcgatatatt tgaatgctcg ctgtggatgc
    57061 tcaccaaata ttttataaag gtttttaatt tctaatttaa ttgccatgca atagaaagat
    57121 tcctgttatt tgtttatgtc gatatattac ccaatggaaa tagttacttt tttctaccct
    57181 aacatactga gaatctgagg caacccctga tggcaaatgt ggcatgaata ttgatgcgag
    57241 caaatcgccg gaatttcccg tgatataagg gctgagagca aatcgagaaa agttgatatt
    57301 ttttgtcaaa gatagcgaaa aaacgcctga atattgtggt ttcaggcgaa taatgcaaca
    57361 gagtatcagt gtagatcacc acaaaatatt tgcgtgataa aaagctattt ggcgggaatt
    57421 atttccctgt cgcgggtagt gatatttttg aaaataacac cctcagaaat tccagtcttc
    57481 atcttctgtt tcaaccgctt tccccatcac ataagaggag cctgaaccgg aaaaaaaatc
    57541 gtgattttca tcggcattcg gcgaaagcgc ggcgaggatt gccggattca cttccgccat
    57601 ttctgcggga aataaaggtt cgtagcccag attcatcaaa gccttattgg cgttgtaaca
    57661 gagaaacgct ttcacatcgt cagcccacgg ggtttcggcg tacagctcat cggtgtattg
    57721 cagctcgttg tcgtagagtt ccagcaacaa atcgaaggcg aaactcttca actcttcacg
    57781 ttgtcccaga gatatctttt ccatgttttt ctgatattta tagcctatgt agtaaccgtg
    57841 gactgcttca tcacggataa tcagacggat caggtctgcg gtattggtca gctttccgcg
    57901 gctggaaaaa tacatcggca gccagaaacc ggaatagaac aaaaaagatt caagaaacac
    57961 actggcgatt ttctttttca gcggatcatc accgcgataa tgttgctgaa taatctgagc
    58021 ttttcgctgc aacggtgcgt tttcttcact ccaggcgtag gcggcatcga catctttggt
    58081 ctggcacagc gtcgagaaaa tcgaactgta agagcgggca tgaaccgctt ccataaagct
    58141 gatattcgat aataccgctt cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc
    58201 gccgataaca ttttgcagcg tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag
    58261 ttgttgttct acgacagtta atgtctgcca ggcaggaata tcgttcgaca gcggcacctt
    58321 ttctggtagc cagaaattgc tggtcaggcg attccacacc tccagatctt tatcgtcaga
    58381 tatcttgttc cagttgatgg cgctgatacg tgagagtttc atagatattc cttaaagtgc
    58441 acaggagacg cagccttcaa tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta
    58501 atagagcgtt ttgatacctt tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt
    58561 ggtggcggta tcggggaaaa acagcgtcag cgacaaaccc tgatcgacat ggcgagtcgc
    58621 ttccgcgtag gtgtcgatga tcttttctgc gccgatttcg taagcatcct gatacagcgc
    58681 cagattttcg ttagtcataa acggggcagg gtagtaaacg cgtcctgttt tgccctcttt
    58741 gcgtatctct actttcgcca caatcggatg aatactcgac gtagcatggt tgatataaga
    58801 gatagaaccg gttggcggca ccgcctgaag attctggttg taaatgccgt agcgcatcac
    58861 gtcgtcgcgc agttgcgccc acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa
    58921 cagttcgcca actttcgccg ttttcggctg ccagttacct tgcagatatt ggctaaaata
    58981 ttcaccactg gcataacgtg actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc
    59041 cagcaacatc gaggtacgca gtgcgtgcca ggtgatggca tagaaataga gattggtgaa
    59101 atccagtgct tccggcgaac cataagcgat gccttctcgc gccagatagc cgtgtaaatt
    59161 catctgcccc agtccgatgg cgtgtgaggc ggcatttccg gcttcgatgg acggcacgct
    59221 gcggatatga ctcatatctg acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg
    59281 ggcaaaatcg ggggaatcca tggtgtgcgc aatattcaac gaacctaaat tgcaggagat
    59341 atcatggcct gtgcgggcat agtcgagatt ctcgtcatac tctgaggcac agttgacctg
    59401 caaaatctct gagcagagat tactcatatt tatgcgcccg gcgacagggt tagcacggtt
    59461 taccgtgtct tcatacatga tgtagggata gccggactca aactggattt ctgccagccg
    59521 ctggaagaaa tcacgggcgt tgaggtattt tttgcggatg cgttcatcgg caaccagttc
    59581 gtcatagtgt tcgctgatgg cgatatcggc aaacggcttg ccataaactc gctcaacgtc
    59641 atagggcgaa aacagcgcca tctgcgcatt ctcttttgcc agatggaaag tgatatccgg
    59701 gatcaccacg cccagcgaca gtgttttaat gcggattttt tcgtcggcat tttcccgttt
    59761 tgtatcgaga aaacgcagaa tatcgggatg atgagcatgt aaatagactg cacctgcccc
    59821 ctgacgcgcg ccgagttggt tggcatagga aaatgcgtct tccagcattt tcatcaccgg
    59881 aattacgcca gaagattgat tttcaatacg tttaattggc gcgcccgctt ctcgcagatt
    59941 cgacagcaaa aatgccacgc cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc
    60001 ccggccaatc gactccatat tgtcttcaat acgcagcaga aaacaggaaa ccagttcgcc
    60061 gcgctgctgc ttaccgcagt tgaggaatgt cggcgtggct ggctgaaagc gtcctgacag
    60121 catttcattg gtcagttgca acgccagcgt cgcatcgcct tgtgccagcg tcagcgccac
    60181 catcgtgact cgatcggcaa aatcttccag ataacgttta ccgtcgaatg tcttcaacgt
    60241 atagctggtg taaaacttcc atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg
    60301 tgcgtgggca aacagcgtaa tgacaaagtc gcgagagtag cgattaagaa cgctttcatc
    60361 atagtaacct tcgttgacca gccagttcag gcgctgctgc tggctactga aggtcacact
    60421 gttcggacgc acatgcgtcg ccataaaggc gtcaacggcc tggcggtctt tatcgaactg
    60481 aatgcgacct gcgctatcgt agaggttaag catcgcattc agcgcgtggt aatccatcgt
    60541 ttcctgcgtc aggcattctg cggttgtcgt tgccaaaatt cggttactcc tttacgaacg
    60601 ttttcgatat cgctttgcgt acccatgagt tcaaaacggt acagccacgg cacgccgcat
    60661 ttccgggcaa tcacatctcc ggcgcggcca tacgcctcac caaagttgcg attaccagaa
    60721 gcaataacgc cgcgaagcaa cgcccggttg tgctcgtcgt ttaaaaagcg aattacctat
    60781 cgtggcaccg cgccagccgt accgccgccg ccgtaagagg gcacgatcag gatgtaaggc
    60841 tcgtctacct gaatccgttc ccgctcattg agcgggatgc gcaccgcggg cagacctaaa
    60901 cgttcgataa aacgctgcgt gttttcggag ctgctggaga agtagacgag ctggctcatg
    60961 cactggccgc gtgtggcgct ggatgcagac ggttaatcat gtccggacgg aaaccagacc
    61021 agctaagatc gccagcaatc actaccggca actgacgaaa gccctgagca cgcaacgctt
    61081 ccgctgcttc aggaacgcga tcgacattaa tcatttcaaa atcaaagccc cggttttcca
    61141 tcgcccgttt ggtggcgtgg cactgaacgc aatcgttacg agtgtaaata gttatgcgca
    61201 tgattcgtat ttccgtttaa aatgaagata cggcgcgatg atacgcgtcg ggttgtctct
    61261 ctgttgatac agagatacta gatgtagttg aaaaaagatt caaccacaca atatatagca
    61321 aatagtgatc gaaattaccc tagatatgag cgtgacgggg gagggggatt tttgtgattc
    61381 accaggcaaa aagaaacccc gaagacaggc ttcggggtta aagacgcgta tttattatca
    61441 tttttgcact acgatttgcg catgcttaac agtgcgccga taaaaatacc taccgcagct
    61501 gctgtaccca cgctacacca gggtctttca cgaacaaaag aatccgcgca gccaacggca
    61561 tcgcgtgcgg cttgctggac gcgagtacga ccatgcattc gcgccctggt ttctttcagc
    61621 aatgcctgag ctttactgcg tgcggcttca gcttcccctt tggcgtcgct gccccaggac
    61681 ttcaatacag attcgaggct gtcagctaat tgattgacat cattctggat atcctgcacg
    61741 ccatcatcaa cgtcgttgcg gttcggtcgg ttaaacatat gatcccccat tagaattcga
    61801 tgtaattttc agtgtagacc ataattttct taactgaggt tcatcgcggc tttatggact
    61861 tttctgaaag cgttgtgaat gcgcaatcag gtaaggtagg cccgcggttg ataataacga
    61921 ggatcaaata tgtatttacg accagacgag gtggcgcgcg tacttgaaaa agtcggtttt
    61981 actgtcgatg tggtaaccca gaaagcttat ggttaccgcc gtggggaaaa ttatgtctat
    62041 gttaatcgcg aagcgcgaat gggacgtacc gcactggtaa ttcatccaac attaaaagaa
    62101 cgtagttcga cactggctga acccgcttcc gatattaaaa cttgcgatca ttatcaacaa
    62161 tttccgctct atttagcagg cgagcgacac gagcattacg gtatcccgca tggcttcagt
    62221 tcgcgtgttg cgcttgaacg ttatttgaat ggtttatttg gcgaagccag ttaaagacgc
    62281 gactggcgat gccagtcgcg aaaagaagag tcaggctttt acctgctggt aatggctgac
    62341 tttaaacagt cggcggcaat aatcgaggaa gtagccataa accgccccca tcaacatcga
    62401 aacaacgatg tttgaactga ccgccgccat aatctgatgc cagtctgcgc ccaccactaa
    62461 caagatcgcc acatacaccg gtgactggaa cgtcacataa gccaggatat cagccagatt
    62521 ttttatccag cccgacgggc taactttgcg tgccgcgcgc ataaatagat cgcggtacat
    62581 accgtatggc catgcaatta agatgttcac cggaatcgct accaatctgg aataaaaaga
    62641 ctgttcgaag ctcattccgg agaggaaaac ttcaatacac atgttcacga cagaacagta
    62701 aacaaccatc gcgaacgtat ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat
    62761 gctaatgctc cttaataaaa gcgtaatggg atgttaatgg agatgaaagc agtggatctc
    62821 attgccatct gcattttcgc atagcgtata tcgctggcta tgactattca attagtgaaa
    62881 aatttttatt tagtcagttt ttgtagataa aatattccgt aacggctcgt tttttgtaca
    62941 ttgtggattt ttacatgctg atatgattta ttttcaatta aatcaattag atatgacctt
    63001 gcctttaatt tcactaatga aagtttgggg gtgaggtaac gctataagcg tagagaattc
    63061 ggtgtatgtc gtgaccgtgt catttacagc atttttgatg ccagaaaatg tacattaaca
    63121 gcagaaagca ccagtgaggg ggg
//
LOCUS       DAEEBB010000006        60958 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEEBB010000006 DAEEBB010000000
VERSION     DAEEBB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.006"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..468
                     /locus_tag="KLF07_000374"
     CDS             <1..468
                     /locus_tag="KLF07_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9830494.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="KLF07_000375"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="KLF07_000375"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBD9830495.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="KLF07_000376"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="KLF07_000376"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBD9830496.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="KLF07_000377"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="KLF07_000377"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBD9830497.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="KLF07_000378"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="KLF07_000378"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBD9830498.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="KLF07_000379"
     CDS             complement(4084..5313)
                     /locus_tag="KLF07_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD9830499.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="KLF07_000380"
     CDS             complement(5377..5817)
                     /locus_tag="KLF07_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD9830500.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="KLF07_000381"
     CDS             complement(5922..6692)
                     /locus_tag="KLF07_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9830501.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="KLF07_000382"
     CDS             complement(6689..7615)
                     /locus_tag="KLF07_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9830502.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="KLF07_000383"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="KLF07_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBD9830503.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="KLF07_000384"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="KLF07_000384"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBD9830504.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="KLF07_000385"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="KLF07_000385"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBD9830505.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="KLF07_000386"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="KLF07_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBD9830506.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="KLF07_000387"
     CDS             13322..13669
                     /locus_tag="KLF07_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBD9830507.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="KLF07_000388"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="KLF07_000388"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBD9830508.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="KLF07_000389"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="KLF07_000389"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBD9830509.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="KLF07_000390"
     CDS             complement(15489..15851)
                     /locus_tag="KLF07_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBD9830510.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="KLF07_000391"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="KLF07_000391"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBD9830511.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="KLF07_000392"
     CDS             18978..20735
                     /locus_tag="KLF07_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBD9830512.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="KLF07_000393"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="KLF07_000393"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBD9830513.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="KLF07_000394"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="KLF07_000394"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBD9830514.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="KLF07_000395"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="KLF07_000395"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBD9830515.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="KLF07_000396"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="KLF07_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBD9830516.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="KLF07_000397"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="KLF07_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBD9830517.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="KLF07_000398"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="KLF07_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBD9830518.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="KLF07_000399"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="KLF07_000399"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBD9830519.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="KLF07_000400"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="KLF07_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBD9830520.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="KLF07_000401"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="KLF07_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBD9830521.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="KLF07_000402"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="KLF07_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="KLF07_000403"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="KLF07_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="KLF07_000404"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="KLF07_000404"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBD9830522.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="KLF07_000405"
     CDS             complement(36855..36899)
                     /locus_tag="KLF07_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830523.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="KLF07_000406"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="KLF07_000406"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBD9830524.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="KLF07_000407"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="KLF07_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBD9830525.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="KLF07_000408"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="KLF07_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBD9830526.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="KLF07_000409"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="KLF07_000409"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBD9830527.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="KLF07_000410"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="KLF07_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBD9830528.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="KLF07_000411"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="KLF07_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBD9830529.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="KLF07_000412"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="KLF07_000412"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBD9830530.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="KLF07_000413"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="KLF07_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBD9830531.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="KLF07_000414"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="KLF07_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBD9830532.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="KLF07_000415"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="KLF07_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBD9830533.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="KLF07_000416"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="KLF07_000416"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD9830534.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="KLF07_000417"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="KLF07_000417"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBD9830535.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="KLF07_000418"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="KLF07_000418"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBD9830536.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="KLF07_000419"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="KLF07_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBD9830537.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="KLF07_000420"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="KLF07_000420"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBD9830538.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="KLF07_000421"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="KLF07_000421"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBD9830539.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="KLF07_000422"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="KLF07_000422"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBD9830540.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="KLF07_000423"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="KLF07_000423"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBD9830541.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="KLF07_000424"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="KLF07_000424"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBD9830542.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="KLF07_000425"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="KLF07_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBD9830543.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="KLF07_000426"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="KLF07_000426"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBD9830544.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="KLF07_000427"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="KLF07_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBD9830545.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16854 c        14998 g        14510 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacg
//
LOCUS       DAEEBB010000007        59841 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEEBB010000007 DAEEBB010000000
VERSION     DAEEBB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.007"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..262)
                     /locus_tag="KLF07_000428"
     CDS             complement(<1..262)
                     /locus_tag="KLF07_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD9830546.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="KLF07_000429"
     CDS             369..665
                     /locus_tag="KLF07_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9830547.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="KLF07_000430"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="KLF07_000430"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBD9830548.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="KLF07_000431"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="KLF07_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBD9830549.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="KLF07_000432"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="KLF07_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBD9830550.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="KLF07_000433"
     CDS             complement(3472..3645)
                     /locus_tag="KLF07_000433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830551.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="KLF07_000434"
     CDS             3673..3756
                     /locus_tag="KLF07_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBD9830552.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="KLF07_000435"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="KLF07_000435"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBD9830553.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="KLF07_000436"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="KLF07_000436"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBD9830554.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="KLF07_000437"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="KLF07_000437"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBD9830555.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="KLF07_000438"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="KLF07_000438"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBD9830556.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="KLF07_000439"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="KLF07_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBD9830557.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="KLF07_000440"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="KLF07_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBD9830558.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="KLF07_000441"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="KLF07_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBD9830559.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="KLF07_000442"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="KLF07_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBD9830560.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="KLF07_000443"
     CDS             14052..15254
                     /locus_tag="KLF07_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBD9830561.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="KLF07_000444"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="KLF07_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="KLF07_000445"
     CDS             16953..18560
                     /locus_tag="KLF07_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD9830562.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="KLF07_000446"
     CDS             18822..20444
                     /locus_tag="KLF07_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD9830563.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="KLF07_000447"
     CDS             20810..21877
                     /locus_tag="KLF07_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD9830564.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="KLF07_000448"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="KLF07_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBD9830565.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="KLF07_000449"
     CDS             24609..25733
                     /locus_tag="KLF07_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9830566.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="KLF07_000450"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="KLF07_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="KLF07_000451"
     CDS             26076..26435
                     /locus_tag="KLF07_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBD9830567.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="KLF07_000452"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="KLF07_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBD9830568.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="KLF07_000453"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="KLF07_000453"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBD9830569.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="KLF07_000454"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="KLF07_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBD9830570.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="KLF07_000455"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="KLF07_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBD9830571.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="KLF07_000456"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="KLF07_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBD9830572.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="KLF07_000457"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="KLF07_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBD9830573.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="KLF07_000458"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="KLF07_000458"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBD9830574.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="KLF07_000459"
     CDS             complement(32630..33679)
                     /locus_tag="KLF07_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD9830575.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="KLF07_000460"
     CDS             33811..35028
                     /locus_tag="KLF07_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9830576.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="KLF07_000461"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="KLF07_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBD9830577.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="KLF07_000462"
     CDS             complement(35662..36327)
                     /locus_tag="KLF07_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBD9830578.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="KLF07_000463"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="KLF07_000463"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBD9830579.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="KLF07_000464"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="KLF07_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBD9830580.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="KLF07_000465"
     CDS             complement(39167..39793)
                     /locus_tag="KLF07_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBD9830581.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="KLF07_000466"
     CDS             39934..40293
                     /locus_tag="KLF07_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBD9830582.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="KLF07_000467"
     CDS             complement(40296..40565)
                     /locus_tag="KLF07_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBD9830583.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="KLF07_000468"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="KLF07_000468"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBD9830584.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="KLF07_000469"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="KLF07_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBD9830585.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="KLF07_000470"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="KLF07_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBD9830586.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="KLF07_000471"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="KLF07_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBD9830587.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="KLF07_000472"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="KLF07_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBD9830588.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="KLF07_000473"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="KLF07_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBD9830589.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="KLF07_000474"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="KLF07_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBD9830590.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="KLF07_000475"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="KLF07_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBD9830591.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="KLF07_000476"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="KLF07_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBD9830592.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="KLF07_000477"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="KLF07_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBD9830593.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="KLF07_000478"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="KLF07_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBD9830594.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="KLF07_000479"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="KLF07_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBD9830595.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="KLF07_000480"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="KLF07_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="KLF07_000481"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="KLF07_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBD9830596.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="KLF07_000482"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="KLF07_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBD9830597.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSKK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="KLF07_000483"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="KLF07_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBD9830598.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="KLF07_000484"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="KLF07_000484"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD9830599.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="KLF07_000485"
     CDS             complement(57808..58248)
                     /locus_tag="KLF07_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBD9830600.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59841
                     /gene="ggt"
                     /locus_tag="KLF07_000486"
     CDS             58368..>59841
                     /gene="ggt"
                     /locus_tag="KLF07_000486"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBD9830601.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14595 a        15113 c        16138 g        13995 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt aagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtgg
    59581 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    59641 ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg aacatttacg
    59701 ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcatcgatgt
    59761 caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt cctggcggga
    59821 gtcggattat taccaccgtg c
//
LOCUS       DAEEBB010000008        57431 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEEBB010000008 DAEEBB010000000
VERSION     DAEEBB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.008"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            146..1489
                     /gene="argG"
                     /locus_tag="KLF07_000487"
     CDS             146..1489
                     /gene="argG"
                     /locus_tag="KLF07_000487"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBD9830602.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1497..3104)
                     /locus_tag="KLF07_000488"
     CDS             complement(1497..3104)
                     /locus_tag="KLF07_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD9830603.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="KLF07_000489"
     tRNA            complement(3582..3668)
                     /locus_tag="KLF07_000489"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="KLF07_000490"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="KLF07_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBD9830604.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="KLF07_000491"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="KLF07_000491"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBD9830605.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="KLF07_000492"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="KLF07_000492"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD9830606.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="KLF07_000493"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="KLF07_000493"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBD9830607.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="KLF07_000494"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="KLF07_000494"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBD9830608.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="KLF07_000495"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="KLF07_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBD9830609.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="KLF07_000496"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="KLF07_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBD9830610.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="KLF07_000497"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="KLF07_000497"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD9830611.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="KLF07_000498"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="KLF07_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBD9830612.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="KLF07_000499"
     CDS             complement(13280..14245)
                     /locus_tag="KLF07_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD9830613.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="KLF07_000500"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="KLF07_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBD9830614.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="KLF07_000501"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="KLF07_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBD9830615.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="KLF07_000502"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="KLF07_000502"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBD9830616.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16420..16698
                     /gene="sfsB"
                     /locus_tag="KLF07_000503"
     CDS             16420..16698
                     /gene="sfsB"
                     /locus_tag="KLF07_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBD9830617.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16746..18005)
                     /gene="murA"
                     /locus_tag="KLF07_000504"
     CDS             complement(16746..18005)
                     /gene="murA"
                     /locus_tag="KLF07_000504"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBD9830618.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="KLF07_000505"
     CDS             complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="KLF07_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBD9830619.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="KLF07_000506"
     CDS             complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="KLF07_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBD9830620.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="KLF07_000507"
     CDS             complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="KLF07_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBD9830621.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="KLF07_000508"
     CDS             complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="KLF07_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBD9830622.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="KLF07_000509"
     CDS             complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="KLF07_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBD9830623.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="KLF07_000510"
     CDS             complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="KLF07_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBD9830624.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21786..22763
                     /locus_tag="KLF07_000511"
     CDS             21786..22763
                     /locus_tag="KLF07_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBD9830625.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22777..23763
                     /gene="kdsD"
                     /locus_tag="KLF07_000512"
     CDS             22777..23763
                     /gene="kdsD"
                     /locus_tag="KLF07_000512"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBD9830626.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23784..24350
                     /gene="kdsC"
                     /locus_tag="KLF07_000513"
     CDS             23784..24350
                     /gene="kdsC"
                     /locus_tag="KLF07_000513"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBD9830627.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24347..24922
                     /gene="lptC"
                     /locus_tag="KLF07_000514"
     CDS             24347..24922
                     /gene="lptC"
                     /locus_tag="KLF07_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBD9830628.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24891..25448
                     /gene="lptA"
                     /locus_tag="KLF07_000515"
     CDS             24891..25448
                     /gene="lptA"
                     /locus_tag="KLF07_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBD9830629.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25455..26180
                     /gene="lptB"
                     /locus_tag="KLF07_000516"
     CDS             25455..26180
                     /gene="lptB"
                     /locus_tag="KLF07_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBD9830630.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26228..27661
                     /gene="rpoN"
                     /locus_tag="KLF07_000517"
     CDS             26228..27661
                     /gene="rpoN"
                     /locus_tag="KLF07_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBD9830631.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27684..27971
                     /gene="hpf"
                     /locus_tag="KLF07_000518"
     CDS             27684..27971
                     /gene="hpf"
                     /locus_tag="KLF07_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBD9830632.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28089..28580
                     /gene="ptsN"
                     /locus_tag="KLF07_000519"
     CDS             28089..28580
                     /gene="ptsN"
                     /locus_tag="KLF07_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBD9830633.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28626..29480
                     /gene="rapZ"
                     /locus_tag="KLF07_000520"
     CDS             28626..29480
                     /gene="rapZ"
                     /locus_tag="KLF07_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBD9830634.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29477..29749
                     /gene="npr"
                     /locus_tag="KLF07_000521"
     CDS             29477..29749
                     /gene="npr"
                     /locus_tag="KLF07_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBD9830635.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29963..30595
                     /gene="yrbL"
                     /locus_tag="KLF07_000522"
     CDS             29963..30595
                     /gene="yrbL"
                     /locus_tag="KLF07_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBD9830636.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="KLF07_000523"
     CDS             complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="KLF07_000523"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBD9830637.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="KLF07_000524"
     CDS             complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="KLF07_000524"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBD9830638.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="KLF07_000525"
     CDS             complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="KLF07_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBD9830639.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34632..35561)
                     /locus_tag="KLF07_000526"
     CDS             complement(34632..35561)
                     /locus_tag="KLF07_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBD9830640.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36236..40696
                     /gene="gltB"
                     /locus_tag="KLF07_000527"
     CDS             36236..40696
                     /gene="gltB"
                     /locus_tag="KLF07_000527"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBD9830641.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40709..42127
                     /gene="gltD"
                     /locus_tag="KLF07_000528"
     CDS             40709..42127
                     /gene="gltD"
                     /locus_tag="KLF07_000528"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBD9830642.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42311..42790
                     /locus_tag="KLF07_000529"
     CDS             42311..42790
                     /locus_tag="KLF07_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBD9830643.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42807..43112
                     /locus_tag="KLF07_000530"
     CDS             42807..43112
                     /locus_tag="KLF07_000530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9830644.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43188..43652)
                     /locus_tag="KLF07_000531"
     CDS             complement(43188..43652)
                     /locus_tag="KLF07_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD9830645.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43649..44524)
                     /gene="nanK"
                     /locus_tag="KLF07_000532"
     CDS             complement(43649..44524)
                     /gene="nanK"
                     /locus_tag="KLF07_000532"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBD9830646.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44521..45210)
                     /locus_tag="KLF07_000533"
     CDS             complement(44521..45210)
                     /locus_tag="KLF07_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBD9830647.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="KLF07_000534"
     CDS             complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="KLF07_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBD9830648.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="KLF07_000535"
     CDS             complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="KLF07_000535"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBD9830649.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="KLF07_000536"
     CDS             complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="KLF07_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBD9830650.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49044..50366
                     /gene="dcuC"
                     /locus_tag="KLF07_000537"
     CDS             49044..50366
                     /gene="dcuC"
                     /locus_tag="KLF07_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD9830651.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="KLF07_000538"
     CDS             complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="KLF07_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBD9830652.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="KLF07_000539"
     CDS             complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="KLF07_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBD9830653.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="KLF07_000540"
     CDS             complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="KLF07_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBD9830654.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="KLF07_000541"
     CDS             complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="KLF07_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBD9830655.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53000..54127)
                     /gene="zapE"
                     /locus_tag="KLF07_000542"
     CDS             complement(53000..54127)
                     /gene="zapE"
                     /locus_tag="KLF07_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBD9830656.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54321..54719
                     /locus_tag="KLF07_000543"
     CDS             54321..54719
                     /locus_tag="KLF07_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBD9830657.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54873..56240
                     /gene="degQ"
                     /locus_tag="KLF07_000544"
     CDS             54873..56240
                     /gene="degQ"
                     /locus_tag="KLF07_000544"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBD9830658.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56330..57397
                     /gene="degS"
                     /locus_tag="KLF07_000545"
     CDS             56330..57397
                     /gene="degS"
                     /locus_tag="KLF07_000545"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBD9830659.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14264 a        15302 c        14255 g        13610 t
ORIGIN      
        1 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
       61 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
      121 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
      181 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
      241 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
      301 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
      361 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
      421 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
      481 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
      541 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
      601 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
      661 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
      721 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
      781 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
      841 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
      901 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
      961 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
     1021 cgaagctccg gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca
     1081 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
     1141 ccaggggcgt tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt
     1201 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
     1261 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
     1321 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat
     1381 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
     1441 cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa
     1501 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
     1561 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
     1621 atcacttcct ccagcgtttt cggttgttcc ggctttgtta cccccatcca cgcattaata
     1681 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtacgatc cacgccatcc
     1741 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
     1801 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
     1861 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
     1921 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
     1981 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
     2041 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
     2101 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
     2161 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
     2221 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
     2281 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgattgatta
     2341 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
     2401 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
     2461 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
     2521 tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca
     2581 aatcgcgacg ctagtatata tggactgaat gcttttttat tttttgcatc tttataagca
     2641 aattgacaag cagaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
     2701 ttttttgtcg gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa
     2761 aaagcaaaag cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
     2821 tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
     2881 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
     2941 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
     3001 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
     3061 acacaaagat acaacaacca atgagattta aaatcttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gataaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtctacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatgacc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaaa gggcgatgaa tctttatata ttctgaatat tcacactctt
    16321 tacaggaact ttttagagca ataggccatc aggagtatag tgatgcccga cagaagaagt
    16381 gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac
    16441 tggcatcccg ctgacatcat tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggag
    16501 tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg cattatcgcg cccatggccg
    16561 aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca
    16621 cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac
    16681 actaaaccga aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca
    16741 gacgattact cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca
    16801 atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca
    16861 atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg
    16921 ccagaaagtt tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcatgcgcg
    16981 cccatacggc taagctccgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg
    17041 aatccggtcc cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg
    17101 aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc
    17161 aggctaatcc agtcttcgcc gacttcgatg tccgctccag cgtcacgcag tttcgccagc
    17221 acggcgtcga gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agaaatcgcc
    17281 gccgccacca ggaaagtacc ggtttcgata cgatccggta gcacgcgata gacaccgccg
    17341 cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca
    17401 cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcattttca
    17461 ataatcgtgg taccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg
    17521 ctgactttat ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg
    17581 taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca
    17641 accggacgcg caccgatcgt acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc
    17701 gctaccagcg gccccagtgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt
    17761 gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc
    17821 gcacccagct ggcttagcag cttcattgat gtatcgacgt ctttcagttt tgggacgttc
    17881 tggatctcta ccggttcttc cgccagtaga gcggcaaaaa ggataggcag agcagcattt
    17941 ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta
    18001 tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct
    18061 caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac
    18121 acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt
    18181 tttttaaccc gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg
    18241 ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt
    18301 tcattatttt ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc
    18361 aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact
    18421 tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga
    18481 ggcagaacat cagcaggcaa attatacaat ttcgcgaggg tatacacttt gtcgtttacc
    18541 ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt
    18601 gccagtccac ccgtatccac gcgggacaca cggctaagat cgatgcaggt aatccccttc
    18661 accgcttcct cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat
    18721 aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt
    18781 ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt
    18841 tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag
    18901 caatcatgtc gtaagcctgc cagtttcccg tctgggagtt tttacgccac tggaagtcca
    18961 gacgcaccgg cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt
    19021 tatcgcccag cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg
    19081 cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag
    19141 caggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta
    19201 cgtatggcag cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt
    19261 gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat
    19321 aaggattggt ctggtctgcc gcggttgccg cactcagagg tgcaatcacc agcaaagcga
    19381 ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag
    19441 gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt
    19501 catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact
    19561 tagtgtcctg aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa
    19621 aaccgacgtt taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg
    19681 aactggtatc tggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat
    19741 aggttttcgg gtccagcgta atatccgcca cccgacccac aacaacgcca ccaatactga
    19801 ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg
    19861 gttcagtacg tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca
    19921 gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa
    19981 ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg
    20041 acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggt gtcgtagccg
    20101 ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg
    20161 gccagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca
    20221 atgcctttcc aactgacgcc gaccagagat ccgccccaga tccccacggc gacgaaaata
    20281 accgtcaaca gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc
    20341 agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg
    20401 atttctgcgg ttagcgccga accagcacgc ccggcaaaca acaacgcggc aaccaccggc
    20461 cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa
    20521 gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaacc
    20581 acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca
    20641 tgtttgcgaa attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg
    20701 aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg
    20761 agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg
    20821 aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg
    20881 caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc
    20941 cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc
    21001 gctgttcagc tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac
    21061 aaaaggttca tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc
    21121 acgccgcgcc atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc
    21181 cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggta gttgggtatg
    21241 ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga
    21301 ctgaaataac atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg
    21361 aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag
    21421 acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg
    21481 cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc
    21541 gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga
    21601 ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg
    21661 ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag
    21721 aatccgttac ggctttctga aaatcttcag cggaccgacg agtatacctg aagaaaggac
    21781 gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg
    21841 ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga
    21901 tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg
    21961 ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta
    22021 tcaatatttt gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattccg
    22081 atgttctgcg tcgtgaatta cccttaatgt tgttggtcag cgtagtggcc ggttccgtac
    22141 tctatgacgg acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat
    22201 ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga
    22261 ccagagagca gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc
    22321 tcggcattgc gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg
    22381 tgctggcgaa ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg
    22441 gaaccagcct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca
    22501 ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac
    22561 ccgcgctgat aacgccagga gagattgagc cattggcgta cagtcgtgac tacagcgtga
    22621 tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc
    22681 gtggtgtagg ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt
    22741 tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg
    22801 ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg
    22861 gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt
    22921 aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc
    22981 gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc atcctggtga agccgcgcat
    23041 ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa
    23101 tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc
    23161 atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa
    23221 gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg
    23281 gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat
    23341 tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat
    23401 attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgcg
    23461 ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg
    23521 attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt
    23581 cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt
    23641 ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc
    23701 gatggcgacc atttactcgg agtgttacat atgcatgatt tactgcgtgc aggcgtagtg
    23761 taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg
    23821 gccctgtcag cgccgacgtt atagcaaaag cagagaacat tcgtctgctg atcctcgatg
    23881 tcgatggcgt actgtcagat ggcctgattt atatgggcaa taatggcgaa gagctgaaag
    23941 cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg
    24001 ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca
    24061 ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac
    24121 tggcgattgc cccggaaaat gtggcttatg tcggcgatga tctcatcgac tggccggtaa
    24181 tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg
    24241 ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat
    24301 tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag
    24361 acgttgggtt atcattgtgc tatcactggc ggttctggtg atgatcggca ttgatatggc
    24421 cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga
    24481 gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca
    24541 acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac
    24601 gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa
    24661 tgaccggatg ctctatttat atggacacgt tgaagtcaac gcacttgtgc caaactctca
    24721 acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc
    24781 tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg
    24841 caacttacgc agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca
    24901 aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc
    24961 cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat
    25021 ctcttgatat gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca
    25081 ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag
    25141 aagtgattga cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac
    25201 ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga
    25261 cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc
    25321 tggtaaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc
    25381 tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg
    25441 gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc
    25501 gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg
    25561 ggccaaacgg tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg
    25621 atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg
    25681 cgcgccgcgg tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt
    25741 acgataacct gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag
    25801 accgcgcgaa cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc
    25861 agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc
    25921 cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca
    25981 ttaaacgcat cattgagcac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca
    26041 acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga
    26101 tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg
    26161 gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat
    26221 tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca
    26281 gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca
    26341 gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac
    26401 ccgcgaaacg caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat
    26461 gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc
    26521 cggcaccagc ggtgactaca ttgacgatga gctgccggtc tatcagggcg aaacgacgca
    26581 gaccttgcag gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg
    26641 cgctattgct acctctatcg tcgatgccgt tgatgacatc ggttatctga ctgtcccgct
    26701 ggaagatatt ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt
    26761 ccttaagcgg atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg
    26821 cctgctaatc caactctccc aattcgataa aaccacgccg tggctggaag aggccagact
    26881 gatcattagc gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt
    26941 cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc
    27001 gcgccccggg cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt
    27061 gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca
    27121 aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt
    27181 tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga
    27241 tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg
    27301 tgaagaatat atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga
    27361 atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga
    27421 actgaagtat ttcttttcca gtcacatcaa taccgagggc ggcggcgaag cttcctccac
    27481 ggcgattcgt gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag
    27541 cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt
    27601 tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg
    27661 acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca
    27721 ccgaggcact gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc
    27781 gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa
    27841 cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg
    27901 ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa gataaactga
    27961 aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg
    28021 ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag
    28081 gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat
    28141 gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc
    28201 tggcggcgaa acaacttagc ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg
    28261 aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc aaactggaag
    28321 aagatactct gcgcgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg
    28381 ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgtcggca gaccaaacta
    28441 aaacgcacct gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc
    28501 gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag
    28561 gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac
    28621 agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc
    28681 gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg
    28741 atctggctcg aaccctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta
    28801 atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct
    28861 caccgcaact cctgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca
    28921 cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag
    28981 aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa
    29041 tgtccgttca cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg
    29101 aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt
    29161 acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga
    29221 caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta
    29281 tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta
    29341 gctacttgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag
    29401 agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc
    29461 tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat
    29521 gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct
    29581 gttacgtaat gacgaaggca ccgaggccga agccaacagc gttattgcgc tgctgatgtt
    29641 ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggcccacagg aagaggaagc
    29701 actggccgcc gttatcgccc tctttaactc tggttttgat gaagattaat cttcatcact
    29761 ttgacataca actcccttca aactcccccc gacaataaga aaatcacgta ctgaaatcgt
    29821 tctcaatcaa cgtcatttgt acattttgtg cgcttttcac tttcagaaga accttaagaa
    29881 aaccttaaga ggcattgttt aggttttgtt taagttaatc gaccatactg gagatcgtca
    29941 gaaaatattt ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg
    30001 aagacatcgg aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta
    30061 ccatcgtggc gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct
    30121 tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga
    30181 ttgcggaacc ggttatgtct acgatgtgat tgcagatttt gacggcaaac tctccattac
    30241 gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac tgcgccagct
    30301 actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc
    30361 gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg tctgcgacaa
    30421 cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa
    30481 gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa
    30541 agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag
    30601 ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc
    30661 ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg gattaggtaa
    30721 tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata
    30781 acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta
    30841 aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta atccagcttc
    30901 cagccctttt cgtacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg
    30961 agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt
    31021 ctcaatggaa gcgacatcaa agccccagtg ctcagaaaat ttctgatctt ctgcggcaat
    31081 caccgccagt cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac
    31141 gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa
    31201 gggaacaggc gcaacactaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac
    31261 cattaacagc aaaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat
    31321 tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg
    31381 ttctgcgcca gcatatatgc tggggtggtg acaatcttat tgtcttcatc aaccacgata
    31441 tcatcgacag gacagggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg
    31501 atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg
    31561 gcaatacaca taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt
    31621 aattcacggt caacggtgca ttcgctacca agagtggcaa aattgcttaa attcttcgcc
    31681 gcgccaaacc cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga
    31741 ggacggattt caccgcgcgt tattcgtgcc gcttcaatca gcacgttgcg cgtttccgtc
    31801 atcgcttcgc cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag
    31861 accgcctgcg caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca
    31921 gaaccgtcat agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc
    31981 ctttcgcaat tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt
    32041 taactgattc atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa
    32101 aacagtgctg tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg
    32161 gtgttggcgc agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc
    32221 cacccacgct ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca
    32281 ttcaccgacg ttatcttccc aggcaggaag gtcaggagac tgaatttgct gacccagctg
    32341 ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac
    32401 aatgcctttt ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat
    32461 catcttctca aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata
    32521 ctgttccagc atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac
    32581 cgtactctcc tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg
    32641 aacagaaagc ggcttactca gcacatcatc cattccagca ttgaggtact cttgtttgtc
    32701 tttcagcacg ttagcggtta aggccaccag cggcggtaaa tcctctcgcg gataacgctt
    32761 cgtcagttca cgagagatat ccagcccggt catatctggc aactgaatat ccagtaacac
    32821 caggtcgtat tcgcccggtt taaacatctc tagcgccgcc ttgccggtca tggcgacatc
    32881 aacgctgtta cctaattttt ccagcacaga acgcgcaaca atcacgttca gttcaatgtc
    32941 ttccaccagc agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc
    33001 gacctcttct gctaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg
    33061 ttcgctggta accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag
    33121 accaataccg gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat
    33181 ggcgaaaatt ttatccagct catcctgcgg aatgccgata ccagagtctt ccacttcaaa
    33241 atgcagcata tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa
    33301 tttgacggcg ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat
    33361 gacctgatgc ggtaatggca gcgtcggctc caggttaaag cgcaatcctt tttgttgcgc
    33421 ctgcaatgcg gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggttgatt
    33481 atcaagctgg accttgcgcc gttccatctt atccatgtcg ataatatcgt taaagatatt
    33541 ccccagcgtg acggccgaaa catggatggt cttgagatat ttttcctgct cggcggtgag
    33601 ttcggtatcc agcagaatgc ggctcagacc gacgataccg ttcagcggtg tacgcaattc
    33661 gtgactgatg gtggagataa acgtcgtttt gtcgcggctg gcccgttcaa gcgcatcctg
    33721 ataccgctta cgctcggtaa tgtctcgacc aaagcccatc aaaccgtgac gtttacccac
    33781 gcggtcgtag tacggcactt tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc
    33841 cagccactgt tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat
    33901 gacttttgcg gcggcttccg gtgagtaaac atcaacaggt ttcaggtgaa ccagttgttt
    33961 ttcgcttttt ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc
    34021 gttacgataa aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg
    34081 ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa
    34141 ggtttcctgc agttctgctt ccgctttttc acgaacggcg atttcctgat ttagctgggc
    34201 aatattatct tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt
    34261 ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag
    34321 aaagtagacc gcccacggcg taatcaacaa accaaagaag atagaacgaa taacatcaat
    34381 gctttcgacc tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac
    34441 gacgagggcc agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc
    34501 aacataatac tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct
    34561 gccccaataa tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc
    34621 ccctcaacga cctactccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg
    34681 ccatgttcat tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac
    34741 agaggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc
    34801 ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt aatcctccag ttcaataccg
    34861 ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcaacgga
    34921 tgtagcttta taccgtcaac accggtttcc actacccgct ccagcgtttg cagacattca
    34981 gccagccctt cgcctggcag accgacaatt aaatgcgagc acaccttcag cccgcgctgg
    35041 cgtgccagct gcgtcgtgcg ctgataacag gcgaaatcat gaccgcgatt aatgcgatgt
    35101 agcgttttgt cgtgcacggt ttgcagcccc agctccagcc acacttcgta gccctggtct
    35161 ttatattcgc aaagcagatc cagcaccgca tccggcacgc agtccgggcg cgtgccgaca
    35221 cacaaaccga caatattggc ctggctcacc gcctgttgat acatcgaacg cagcacctga
    35281 acttcggcaa aggtgctggt atacgcctgg aaataggcga ggtaacgttt cgcgcgatta
    35341 attaaattcg cctgatgcgc cagctgttcg gcaatggaac gatgctgctg cgcttcgtcg
    35401 gcaaacgagg caacattaca gaaggtgcag ccgccgcgcc cgatggtgcc gtcgcgattc
    35461 gggcaactga aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg
    35521 agatcaccac caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct
    35581 tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg cgatgacctg gatcaatcgt
    35641 cccggcccgc ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcataatc
    35701 acataaatca cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc
    35761 aaaaacagta gcatgaaacg tcattaccaa ttaatgcagt ataaaatgct ggttttctcg
    35821 tcaattcaag gcaggataag ggttaacacg cctttatgac agtcaggaat tgactgtttc
    35881 tctaacgact tcccttttag ccttaaagat aaaatctatt ttaatttcag ccatttaata
    35941 aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag
    36001 ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata
    36061 tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag
    36121 ggggcgaatg cgaggcgcgc gtatgacacg caacccccgt cgccacgctc tttctgtacc
    36181 cgtgcgcagc ggttcggtag tggggttccc gcagagcctg ggggaggttc acgatatgtt
    36241 gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg
    36301 cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg
    36361 tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc
    36421 ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcga aaaactacgc
    36481 cgtcgggatg ctattcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt
    36541 tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa
    36601 cgaaggcgta ctgggtgaaa tcgccctctc ctctctgccc cgcattgagc aaatttttgt
    36661 gaacgccccg gcaggctggc gtccgcgcga tatggagcgc cgtctgttta tcgcccgccg
    36721 ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc tatcgaatct
    36781 ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct
    36841 tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac
    36901 cgtaccgcgc tggccgctgg cgcaaccgtt ccgctatctg gcgcataacg gtgaaatcaa
    36961 caccatcact ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct
    37021 tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa accggctctg actccagttc
    37081 gatggataac atgctggaac tcctgctggc aggcgggatg gatatcatcc gcgccatgcg
    37141 tctgttagtg ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt
    37201 ctttgacttt aactccatgc atatggagcc gtgggatggc ccggcgggca tcgtgatgtc
    37261 cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt
    37321 catcaccaaa gataagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc
    37381 tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtta tcgacacccg
    37441 cagtgggcat attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccatccata
    37501 taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga
    37561 agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa
    37621 ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc
    37681 ggtcggttcg atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat
    37741 ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg
    37801 tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga
    37861 agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt
    37921 caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt
    37981 tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag ctgtgcgaca aagccgaaaa
    38041 aatggtacgt agcggcaccg tgctgctggt gctctccgac cggaatatcg ctaaagatcg
    38101 cctgccggtt ccagccccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag
    38161 cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt
    38221 cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca taccttgcct atgaaacgct
    38281 gggccgcctg gtagacaccc atgcgattgc caaagattat cgtaccgtga tgctcaacta
    38341 ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat
    38401 cgcctcttac cgctgctcga aactgtttga agcggtcggt ctacacgatg atgtagtggg
    38461 cctgtgcttc cagggggcgg tcagccgcat tggtggggca agctttgaag acttccagca
    38521 ggatctgctg aatctgtcga aacgtgcctg gctggcgcgt aagcccatca gccagggcgg
    38581 cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac
    38641 gctgcaacaa gcggtacaaa gcggcgagta cagcgactat caggaatacg cgaagctggt
    38701 taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc
    38761 ggtcaacatt gctgatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgctgc
    38821 gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaacag
    38881 catcggcggt aactcaaact ccggtgaagg cggtgaagat ccggcgcgct acggcaccaa
    38941 caaagtgtcg cgcatcaagc aggtggcttc cggtcgcttt ggggttactc cggcgtatct
    39001 ggtcaatgcc gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg
    39061 tcagttgccg ggtgataaag tcactcctta catcgccaaa ctgcgctatt cggtgcccgg
    39121 agtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgagg acttagcgca
    39181 gctcattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga agctggtttc
    39241 cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa gcttatgccg atttaatcac
    39301 catcgcaggc tatgacggtg gcaccggcgc aagcccgctg tcgtcagtga aatacgcagg
    39361 ttgcccgtgg gagctggggc ttgttgaaac ccagcaggcg ctggttgcca atggtctgcg
    39421 tcataagatc cgtttgcagg tcgatggcgg cctgaaaacc ggtgttgata tcatcaaagc
    39481 ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtcg cgctcggctg
    39541 taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg ggtgtggcaa ctcaggatga
    39601 caaactgcgt aagaatcact atcacggcct gccgttcaag gtgacgaatt actttgagtt
    39661 tatcgcccgt gaaacccgcg agctgatggc acagctgggg gtaacgcggc tggtggatct
    39721 gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact
    39781 ggcgctgtcg aagctgctgg agaccgccga accgcatcca ggtaaggcac tctactgcac
    39841 cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcgaa
    39901 accgtttgtc gatgaacgcc agagcaaaac cttctggttc gatatccgca ataccgatcg
    39961 ctccgtgggc gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc
    40021 agccgatcct atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtatggaa
    40081 cgcgggcggc gtggaactgt acctgaccgg ggatgccaac gactatgtcg gtaaaggcat
    40141 ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc
    40201 aagtattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg
    40261 ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat
    40321 tggcgacaac ggctgtgaat atatgacggg cggtattgtc tgtattctgg gtaaaaccgg
    40381 cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggtga
    40441 tttccgcaaa cgcgttaacc cggaactggt cgaggtctta agcgttgacg atctggcgat
    40501 ccatgaagag catctgcgcg gtcttatcac cgagcatgtt cagcatactg gctctcagcg
    40561 cggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc tggttaaacc
    40621 gaagtccagt gatgtaaaag cactgctggg tcatcgtagt cgtagcgcag cagagttgcg
    40681 cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct
    40741 gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc aaaattgagt ttgttgaaat
    40801 ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg
    40861 caacccatac tgcgagtgga aatgtccggt acacaactac atcccgaact ggctgaagct
    40921 cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg caccagacca acaccctgcc
    40981 ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc gaaggttcct gcactctgaa
    41041 cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt
    41101 cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat
    41161 tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa
    41221 agccgttgtc ttcgaccgtc atccagaaat tggcgggctg ctgaccttcg gtattccggc
    41281 cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat
    41341 tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag ctggacgatc tgctgagtga
    41401 ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa
    41461 cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt
    41521 aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt
    41581 ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt acgtccgtgc gccagggagc
    41641 gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga
    41701 agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc cactggggat
    41761 tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga
    41821 accggatgcc aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc
    41881 ggcagatgca gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa
    41941 acacagcgtc gagctggatt cacaaggccg catcatcgcc ccggaaggca gcgacaacgc
    42001 cttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct
    42061 ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac ggtattatga actggctgga
    42121 agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt
    42181 tctttctgga aagcgcctcg cataatctga agaaaaccaa tgtaatacgc tcatctggat
    42241 tgttaccggt agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa
    42301 gggacagata atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga
    42361 cggtattatt cacctggtcg atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct
    42421 gatgaccgcg acatactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga
    42481 ggcaagggct gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag
    42541 gtataagcgt ggtttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt
    42601 tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca
    42661 gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaattgag
    42721 aggctgtttg atatttccac agcttttgta ttgcatctcc tattttcggt ttgtatattc
    42781 atgcaaataa acgaaggaaa gttgccgtga tttacattga acagcttgaa ttaatccata
    42841 aaagtggtga tgtgttgtat ccagtaaaaa taaccagaaa atcatcagga aaaactgcct
    42901 tccaccttgt tccattcggc ttaaataaga ctcacgacct tctagaggtg gaagatgctt
    42961 cagaagcaat tcggctggtg attgatgaac gtcattccat tcgctgcagt acacttacag
    43021 caactattac gaataaaaag ggcaagcgaa ttaagcggac aggtatatac agtatcaagg
    43081 gtgttaacat aaaagaatac aacgtcaggt aatggcacag agcggtccgt cagttcaggt
    43141 ttggctctgt gccaaagatg ggtcaggtca cacctgatct aataaattta agccattaaa
    43201 tcagccttaa ccttcaccac caccttttta atctcacccg gctcgccaac aacgcatccc
    43261 ggcttatgcg gctctcctag cataaacacg gcgaacattc ccggtttcag gatgatggct
    43321 tgctcgtttt caatggtgct gcaaagctgg taatcatcct catggtggaa ctcttcacac
    43381 tgacgcgcag tgcctgccat gccaaacaga atccgttctt caccgtttaa taacagctgg
    43441 atatcaatgt attgctcgtg cagctccgct tttttctcaa cgggcgattg agtgttaaac
    43501 gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg cgctttttct
    43561 tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg taacccagca
    43621 gacggtaatg accgtacttc acccatcatc ataatttttc tccctgggcc aacagcgctg
    43681 ccccaagtaa acctgcatca tggcggtagt gcgccgccag taaatcaaca tgaaataccg
    43741 ctggctcctg cgccagatac gtttccacca acgccagata cccttctgcc agaccaacgc
    43801 tgccaccgac caccacgcac tggcaatcag ttgtggcttt aatatcagcg atcagccttg
    43861 ccagcacatg tgcggagcgg tgaatcagtt gctgcgcctg ctcgtcgccc tgcccggcgc
    43921 gtgtgaaaat agttctcgca tccgcgccca tgagttcacc ttgcgctgcc gctgcaatac
    43981 cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc acagccgcag actgggccgt
    44041 gtggatcggc aagcgtatgc ccgatatgcc ccgccagacc gccagggccg gtaagcagtt
    44101 taccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac accatctcgg
    44161 ttacatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg
    44221 caatggtcgg caaattggta agttgttcca gcgttttgac taacggaaag tgtagcaatc
    44281 caccgagatt atgcggattc agcgccagca aactgccgtc gcgaataatt ccggttgaag
    44341 cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat aaggcatcac
    44401 gcaaggcttc tggtgtctgg ctggctggcg taggaagttc acgacgatcg cggatctgcc
    44461 cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc gccagtgtgg
    44521 tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt
    44581 aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc
    44641 aggcgtgttg taacgccctt cggcaatcac ccgacatccg gcttcgctca acgttttcac
    44701 cagcgccaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc
    44761 aataatttcg gctcccagct tttggcatgc caggccgtct tccggcgttg agcagtcggt
    44821 catcgccagt aaaccgtgat gatgtatacg tgccagcagc gtttcaacag gcaccggacg
    44881 cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac
    44941 atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc
    45001 aataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg
    45061 aatggcaacc gcgcccgcct gctctgccgc taatgccatg gctgcgacga tttcgggttt
    45121 atcgagcggg ctgtcaggaa tcggctggca ggagacaatc aggccaccgt tagcagcgat
    45181 tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag gcccggtaca
    45241 tagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc gctgccaaat
    45301 ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg
    45361 cgcagccaac gctgaacgcg ggaaggcata tccagaccaa tcagcaggat caccacaaat
    45421 gtcaggctga acgagagcga tgccagcgca gtacccagat ccagacgttg agcgatcaac
    45481 gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg
    45541 cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa gatcccggcg
    45601 atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca gacgtttgcg
    45661 ccgccaatcg caaataccgg aataatcagc agctgcgagg ccagcaggct acaaacgtac
    45721 gctttgcggg ttcccagcca gtcaccgagg aagcctccta cgcagcatcc cacggccgcg
    45781 ccaaagccac taaagaacag cacattggcg acagtatgcg ggttataagc cagatcggtt
    45841 ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc aaacaacacg
    45901 accaccatca gcattacgcc cgttggccag cgtttgcctg tactctgcac cataaagctg
    45961 ataaagattg cggcgcataa cagcccaaga acagcgacga tcgcggcatt ttgcaggtta
    46021 ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat attggcaatg
    46081 cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt acctgcgtgt
    46141 ttctctttcc agtcttccgc ttccgggatg tttttacgca gccagagagc aaagatgatt
    46201 ggcaaaatgc cgataaagaa cagcgcacgc caaccccaga ccggaaccac caggctatag
    46261 acctgagcgg caacgacggc ccccacagag aagcctgaaa tcaaaaaacc actggctttg
    46321 ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc gtattcaccc
    46381 gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag
    46441 ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc cagacgacgc
    46501 ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga gataaaggct
    46561 gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccctg tacttcggtg
    46621 agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg
    46681 gaaaatgcgc gccattgtgc acggttgaga tggcgatacc acgggatatt ctgggttgta
    46741 gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat gaaaattgta
    46801 gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga aaacaactca
    46861 cccgcgctct tgcatcaact gctgggccag cgccttcagt tctggcagat atttttcatc
    46921 taccggtcca aacggtttgc ggcacagcgg cacagaaacg acatccatat aatgcaggac
    46981 agttttcagg ccgcggaata cgccagtttt gatcagtaaa tcaatgactt tgttgcactc
    47041 agtttgcagt ttctgcgcgg tctggatatc gccttctttc agcgccttca cgatcccctg
    47101 atatcgccag cccatgatgt tgtaagtact gccgatacca ccatcagcgc ctgccagcaa
    47161 gccggaagcg aagatttcgt cgtaaccgtt atagagcacc agatcaggat gttcacgacg
    47221 gatctgttcc atctgataga gatcgccaga agtctgtttc agcgcaccga cgccaggcaa
    47281 cgtaaccagg gtgttgatct gatccagggt cagttttacc ccactcaggg ctggaatgtt
    47341 gtacacaacc atcggcaaac catccgctga atcaataatt gcccgatagt gatcgcagtg
    47401 ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg gagacggcat cgaagccata
    47461 acgtttagcc gatgccgcaa gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc
    47521 gatgagttta atcttacctt tcgcctcttc ggcgacgatt tccagtacct gttcacgctc
    47581 ggaaaggctt tgtacaaagg cctcgccggt cgaaccaccc acgtataaac cgtcgatgcc
    47641 ctgctgaata ttgaactgaa ccaggcgacg cagactcgct ttatccagtg cttgttgttg
    47701 gtcaaaagga gtcaggagtg cagccattac gccacgtaaa ttcgttgcca taaatacctc
    47761 tgaagtgata cttgtctgat aaacgatata cctttatacc tgttatacca gatcaattaa
    47821 gcaacacccc atacagaaag cttataatgc gatctgcttc actaaagtgg cattatttct
    47881 ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt
    47941 gcaacgcacg atcggcttcg tcaggatcat gacggcggat cgcatcaacg atcgcaatat
    48001 gctgttgata gctaacgttg ttatgctcgt gcagagcctg atcagccacg gttggacgtg
    48061 cggcgatcag ccagtcgagc agggcaacgt ggatcgccat gaagattggg ttaccaggga
    48121 tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca
    48181 gcgactgact gttgatttcc agtgcttttg ccagcaaatc gatttgctca tcggtggcat
    48241 gttcagccgc atagcgcacc agactggatt caaagaacag acgtaattgt tcgaaatggg
    48301 caatcccacc gggatgagaa aggaaatctt tcgccatgcc ggaaaactca ccgatgatag
    48361 tgtccgcaga aggacgcgag acgcgagcgc gttcgccgtt gtttatttgc accagacctt
    48421 tgcgttttaa cgctgccagc gcttcgcgca cagaaggacg cccgacgtta aagaacgcca
    48481 tcagttcgcg ttcagacggt aattgttcac cttcgccaaa ttcacgacgg cggatcatct
    48541 gttccagctc ttcttccacc atttcggaga gttttttacg cgccagcggg cggctacgca
    48601 agttgcgacc aattgcaggt gaagaatctt cggtttgcga atcaaatgcg ttcataaggc
    48661 ccattctgta aggtcagtgt gattaacatc atcagtgaca tcctatcaca ggattgaaag
    48721 taggggaaaa tggcaggttt ttctctttgt gcctcatcat taccataatt aacggaataa
    48781 ttaactattg cgaaaaatta atgtaacgca gataaacaca tcccgattga attattcata
    48841 agactattca caagcattat gaatattatg aatgtgttct tacaaaataa tcataagcgc
    48901 atatttttta atgaaaaatc acatcaccta caattaaaaa cacgacatcc gcaccataaa
    48961 tagacttgca aaaaatataa catcattgtt ttcaatctgc cgtttatggg attgaccgtt
    49021 ttcttttgac acggagttca acaatgttcg gcataattat atctgtcatc gtattaatta
    49081 cgatgggcta tttgatcctg aaaaactaca aacctcaggt ggtgctggct gccgcaggtg
    49141 tcttcctgat gatgtgcgga gtctggttag ggttcggtgg tgtactcgat cccgccaaaa
    49201 gcagcggcta cttgatcgtc gacatttata atgaaatcct gcgcatgctg tccaaccgca
    49261 ttgccggatt ggggctgtcg attatggcgg tgggcggtta tgcccgctac atggagcgca
    49321 taggagccag tcgcgcgatg gtgagcttgc taagccgccc gttaaaactc attcgctcgc
    49381 cgtatattat tctgtcggca acttacgtca tcggccaaat catggcgcag tttattacca
    49441 gcgcctccgg tctgggtatg ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg
    49501 gagtaagtcg cctctctgcg gtggcggtta tcgcaaccac gatgtccatt gagtggggga
    49561 ttctggaaac gaactccatt tttgcagccc aggtcgcggg aatgaaaatt gccacatact
    49621 tcttccacta ccagcttccg gtcgcctctt gcgtcattat ctcggtggcg atctcccact
    49681 ttttcgtgca acgcgctttt gacaaaaaag ataaaaatat caatcacgaa caggcagagc
    49741 aaaaaactct cgataatgtc ccgccgctct attacgccat tttacctgtg atgccgttaa
    49801 tcctgatgct cggctcgctg ttcctcgccc acgtcgggct gatgaagtca gaactgcatc
    49861 tggtggtggt gatgttacta agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc
    49921 ataacttgcg cgaaacaatg gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt
    49981 ttgccaacgt ggtaacgctg gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga
    50041 ttggcactgt tgatgcggtt atcaggggtg cggagcattc tggtctgggc ggtattggcg
    50101 tgatgattat tatggcgcta gtcattgcca tttgtgccat tgtgatgggc tctggcaatg
    50161 cgccgtttat gtcatttgcc agtcttattc cgaatatcgc agccggacta catgtaccag
    50221 cggttgtaat gattatgccg atgcattttg ccacgacgct agcgcgcgcg gtttcgccga
    50281 ttactgcggt ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga
    50341 ttgccacaat cacgctattt tattaagtca ttaaaaagac aaaacaggcc gcctgggcct
    50401 gttttgtatt acttcacaac acgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt
    50461 tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata
    50521 acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag
    50581 gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg
    50641 gcagccagcg gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc
    50701 tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc
    50761 ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg
    50821 tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga
    50881 cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacac atttcacgtt
    50941 ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt
    51001 tcagctcttt cgcacccggg ccgctgagct cgatacccag ttgcggcaga cgccacagca
    51061 gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac
    51121 cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt
    51181 cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat
    51241 gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg
    51301 ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat
    51361 ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt
    51421 ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct
    51481 gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag
    51541 cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg
    51601 gccagtcaga agttgtgtta cccaataagg aacgactctc tttgttcgaa aatcaaacaa
    51661 aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg
    51721 accattgtgg tgagtggatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata
    51781 gttgacttag acccttattg gcgatgtggt ttttgttttg cctgtctttc agacggcagc
    51841 aaaaagcaac ttcccatttt ttacgctgat tcagaattta gccataaaaa aacccgccga
    51901 agcgggtttt ttcgtaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac
    51961 tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca
    52021 cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga
    52081 gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg
    52141 gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc
    52201 atgcgggcag tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta
    52261 ccgttgcccg gtttgatgaa aacgcgagct gcggaatttt tgcggcgacc agtgccgtag
    52321 tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt
    52381 gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc
    52441 agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga
    52501 gcaatcatct cttcaaagct cacttgtttg ataccaccga tgtggccggt gtggtgatag
    52561 tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg
    52621 atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgtaggcga
    52681 cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag
    52741 tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa
    52801 tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc
    52861 cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac
    52921 tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt
    52981 ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac tcttcgcttt
    53041 gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat
    53101 aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact
    53161 catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta
    53221 catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc
    53281 gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg
    53341 tcgctaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc ccccccgcca
    53401 gcgccaacca tagtttatcc atttgcgccc gggtttcatc gtggagtggc gaaagccaca
    53461 gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg ttcattacat
    53521 cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aagccatttc
    53581 gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca
    53641 aggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca aaaaattcgt
    53701 caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc agcggatcgg
    53761 tctgcccctg taaggcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca
    53821 ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc
    53881 cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttcgc
    53941 gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg
    54001 tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct ttttgaacgt
    54061 cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc
    54121 tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga
    54181 aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac
    54241 ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca
    54301 aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca
    54361 tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt
    54421 tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta
    54481 gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt
    54541 atcagcacat ggcgaaaagt tccagcagcc tgctgccgga actgtctgct gaagcaaacc
    54601 cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc
    54661 ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat
    54721 ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa
    54781 tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct
    54841 tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaccccag ctgttgagtg
    54901 cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc
    54961 caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc
    55021 ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg
    55081 aagaattcaa aaagtttttt ggtgatgatt taccggagca acctgcacaa cccttcgaag
    55141 gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg
    55201 tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa
    55261 aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa aacccgagca
    55321 aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtag
    55381 cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag
    55441 gacgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta
    55501 accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt ggcatcaaca
    55561 ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc cccagtaata
    55621 tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag
    55681 gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc
    55741 gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaag
    55801 cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct
    55861 ctcgtatcgc gaccaccgag ccgggcacaa aagtgaaact tggcctgctg cgtaacggca
    55921 aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa
    55981 tgatcgcgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca
    56041 aaggtattaa gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa
    56101 aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta
    56161 aagtgctggc ggcaaaatcg gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca
    56221 tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt
    56281 taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa
    56341 gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc
    56401 ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc
    56461 tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg
    56521 tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat
    56581 catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat
    56641 catcgtcgcc ttacaggatg gtcgtgtatt tgaagcattg ctggtgggat ctgactctct
    56701 aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca ttccaatcaa
    56761 tgcacgtcgc gtaccgcata ttggcgacgt ggtactggcg atcggtaacc cgtacaacct
    56821 cgggcagacc attacccagg ggattattag tgccacgggt cgaattggtc tgaacccgac
    56881 cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc
    56941 gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa
    57001 cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat
    57061 tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggca ttggcggacg
    57121 tgagatcgca ccgctgcacg cacagggcgg tggtatagat caactgcaag ggatcgtggt
    57181 taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat
    57241 tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc aggtggcgga
    57301 aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct
    57361 gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa aacaaaaaac
    57421 cggagtctgt g
//
LOCUS       DAEEBB010000009        50276 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEEBB010000009 DAEEBB010000000
VERSION     DAEEBB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.009"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="KLF07_000546"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="KLF07_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBD9830660.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="KLF07_000547"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="KLF07_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBD9830661.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="KLF07_000548"
     CDS             complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="KLF07_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBD9830662.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4218..4622
                     /locus_tag="KLF07_000549"
     CDS             4218..4622
                     /locus_tag="KLF07_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBD9830663.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4648..5361
                     /gene="msyB"
                     /locus_tag="KLF07_000550"
     CDS             4648..5361
                     /gene="msyB"
                     /locus_tag="KLF07_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBD9830664.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5510..6076
                     /gene="satP"
                     /locus_tag="KLF07_000551"
     CDS             5510..6076
                     /gene="satP"
                     /locus_tag="KLF07_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBD9830665.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6111..6698)
                     /gene="mog"
                     /locus_tag="KLF07_000552"
     CDS             complement(6111..6698)
                     /gene="mog"
                     /locus_tag="KLF07_000552"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBD9830666.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6813..7766)
                     /gene="tal"
                     /locus_tag="KLF07_000553"
     CDS             complement(6813..7766)
                     /gene="tal"
                     /locus_tag="KLF07_000553"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD9830667.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8045..9474
                     /locus_tag="KLF07_000554"
                     /pseudo
     CDS             8045..9474
                     /locus_tag="KLF07_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9544..10320
                     /gene="yaaA"
                     /locus_tag="KLF07_000555"
     CDS             9544..10320
                     /gene="yaaA"
                     /locus_tag="KLF07_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBD9830668.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10473..10769)
                     /locus_tag="KLF07_000556"
     CDS             complement(10473..10769)
                     /locus_tag="KLF07_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD9830669.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="KLF07_000557"
     CDS             complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="KLF07_000557"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBD9830670.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="KLF07_000558"
     CDS             complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="KLF07_000558"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBD9830671.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="KLF07_000559"
     CDS             complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="KLF07_000559"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBD9830672.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15693..15812)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="KLF07_000560"
     CDS             complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="KLF07_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBD9830673.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16027..16713)
                     /locus_tag="KLF07_000561"
     CDS             complement(16027..16713)
                     /locus_tag="KLF07_000561"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD9830674.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17113..17253)
                     /locus_tag="KLF07_000562"
     CDS             complement(17113..17253)
                     /locus_tag="KLF07_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830675.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17349..18065
                     /gene="arcA"
                     /locus_tag="KLF07_000563"
     CDS             17349..18065
                     /gene="arcA"
                     /locus_tag="KLF07_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBD9830676.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18125..19477)
                     /gene="creD"
                     /locus_tag="KLF07_000564"
     CDS             complement(18125..19477)
                     /gene="creD"
                     /locus_tag="KLF07_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBD9830677.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19535..20959)
                     /gene="creC"
                     /locus_tag="KLF07_000565"
     CDS             complement(19535..20959)
                     /gene="creC"
                     /locus_tag="KLF07_000565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBD9830678.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20959..21648)
                     /gene="creB"
                     /locus_tag="KLF07_000566"
     CDS             complement(20959..21648)
                     /gene="creB"
                     /locus_tag="KLF07_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBD9830679.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21661..22134)
                     /gene="creA"
                     /locus_tag="KLF07_000567"
     CDS             complement(21661..22134)
                     /gene="creA"
                     /locus_tag="KLF07_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBD9830680.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22345..23214
                     /gene="robA"
                     /locus_tag="KLF07_000568"
     CDS             22345..23214
                     /gene="robA"
                     /locus_tag="KLF07_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBD9830681.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="KLF07_000569"
     CDS             complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="KLF07_000569"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBD9830682.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23910..24431
                     /gene="yjjX"
                     /locus_tag="KLF07_000570"
     CDS             23910..24431
                     /gene="yjjX"
                     /locus_tag="KLF07_000570"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBD9830683.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="KLF07_000571"
     CDS             complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="KLF07_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBD9830684.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="KLF07_000572"
     CDS             complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="KLF07_000572"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBD9830685.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27080..28747
                     /gene="ettA"
                     /locus_tag="KLF07_000573"
     CDS             27080..28747
                     /gene="ettA"
                     /locus_tag="KLF07_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBD9830686.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="KLF07_000574"
     CDS             complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="KLF07_000574"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBD9830687.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30307..31689)
                     /gene="radA"
                     /locus_tag="KLF07_000575"
     CDS             complement(30307..31689)
                     /gene="radA"
                     /locus_tag="KLF07_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBD9830688.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31738..32706)
                     /gene="serB"
                     /locus_tag="KLF07_000576"
     CDS             complement(31738..32706)
                     /gene="serB"
                     /locus_tag="KLF07_000576"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBD9830689.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32812..33456
                     /locus_tag="KLF07_000577"
     CDS             32812..33456
                     /locus_tag="KLF07_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBD9830690.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33484..34500
                     /gene="lplA"
                     /locus_tag="KLF07_000578"
     CDS             33484..34500
                     /gene="lplA"
                     /locus_tag="KLF07_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBD9830691.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34532..34681
                     /locus_tag="KLF07_000579"
     CDS             34532..34681
                     /locus_tag="KLF07_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830692.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34956..35675)
                     /gene="deoD"
                     /locus_tag="KLF07_000580"
     CDS             complement(34956..35675)
                     /gene="deoD"
                     /locus_tag="KLF07_000580"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBD9830693.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35755..36978)
                     /gene="deoB"
                     /locus_tag="KLF07_000581"
     CDS             complement(35755..36978)
                     /gene="deoB"
                     /locus_tag="KLF07_000581"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD9830694.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37030..38352)
                     /gene="deoA"
                     /locus_tag="KLF07_000582"
     CDS             complement(37030..38352)
                     /gene="deoA"
                     /locus_tag="KLF07_000582"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBD9830695.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38479..39258)
                     /gene="deoC"
                     /locus_tag="KLF07_000583"
     CDS             complement(38479..39258)
                     /gene="deoC"
                     /locus_tag="KLF07_000583"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBD9830696.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39516..41066
                     /locus_tag="KLF07_000584"
     CDS             39516..41066
                     /locus_tag="KLF07_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBD9830697.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41038..41901
                     /locus_tag="KLF07_000585"
     CDS             41038..41901
                     /locus_tag="KLF07_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBD9830698.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(42417..43199)
                     /locus_tag="KLF07_000586"
     CDS             complement(42417..43199)
                     /locus_tag="KLF07_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD9830699.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(43196..44269)
                     /locus_tag="KLF07_000587"
     CDS             complement(43196..44269)
                     /locus_tag="KLF07_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBD9830700.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(44391..44552)
                     /locus_tag="KLF07_000588"
     CDS             complement(44391..44552)
                     /locus_tag="KLF07_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBD9830701.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44679..45284)
                     /gene="osmY"
                     /locus_tag="KLF07_000589"
     CDS             complement(44679..45284)
                     /gene="osmY"
                     /locus_tag="KLF07_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBD9830702.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45677..47266)
                     /gene="prfC"
                     /locus_tag="KLF07_000590"
     CDS             complement(45677..47266)
                     /gene="prfC"
                     /locus_tag="KLF07_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBD9830703.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(47357..48034)
                     /gene="yjjG"
                     /locus_tag="KLF07_000591"
     CDS             complement(47357..48034)
                     /gene="yjjG"
                     /locus_tag="KLF07_000591"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBD9830704.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(48049..48495)
                     /gene="rimI"
                     /locus_tag="KLF07_000592"
     CDS             complement(48049..48495)
                     /gene="rimI"
                     /locus_tag="KLF07_000592"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBD9830705.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(48464..48877)
                     /gene="holD"
                     /locus_tag="KLF07_000593"
     CDS             complement(48464..48877)
                     /gene="holD"
                     /locus_tag="KLF07_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBD9830706.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48980..50011
                     /gene="rsmC"
                     /locus_tag="KLF07_000594"
     CDS             48980..50011
                     /gene="rsmC"
                     /locus_tag="KLF07_000594"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBD9830707.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11840 a        13621 c        12691 g        12124 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     2161 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat
     2221 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     2281 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     2341 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt
     2401 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     2461 catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt tcaccctgca
     2521 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     2581 tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     2641 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     2701 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     2761 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     2821 ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt
     2881 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     2941 tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa
     3001 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     3061 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     3121 ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg
     3181 tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     3241 cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca
     3301 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     3361 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     3421 tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt
     3481 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     3541 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     3601 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     3661 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     3721 caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc
     3781 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     3841 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     3901 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     3961 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     4021 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     4081 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     4141 gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc
     4201 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     4261 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     4321 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     4381 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     4441 ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
     4501 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
     4561 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4621 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4681 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     4741 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4801 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
     4861 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     4921 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
     4981 gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag
     5041 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     5101 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
     5161 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     5221 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5281 ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct gcgccggatg
     5341 gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa
     5401 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta
     5461 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5521 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5581 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     5641 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5701 cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg cgattctgct
     5761 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5821 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5881 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     5941 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     6001 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     6061 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     6121 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     6181 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     6241 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     6301 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     6361 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     6421 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6481 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     6541 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6601 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6661 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6721 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     6781 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6841 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6901 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6961 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     7021 agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     7081 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     7141 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     7201 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     7261 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7321 tctttttcca gctgttctgc agcacggata ccctgccaag tagaagccag tttgatcaga
     7381 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7441 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7501 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     7561 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7621 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7681 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7741 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7801 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7861 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7921 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7981 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     8041 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     8101 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     8161 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     8221 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     8281 cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc
     8341 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     8401 tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggggat
     8461 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8521 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt tcccccgctg
     8581 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     8641 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     8701 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8761 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8821 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     8881 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg gattgtccag
     8941 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     9001 ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     9061 atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt tattctgttt
     9121 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9181 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9241 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9301 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
     9361 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9421 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9481 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9541 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9601 acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca tgaagcgcgg
     9661 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
     9721 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggct
     9781 attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac
     9841 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
     9901 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9961 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    10021 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    10081 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    10141 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10201 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10261 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10321 gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10381 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10441 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10501 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccatgt
    10561 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10621 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    10681 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    10741 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10801 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    10861 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    10921 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10981 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    11041 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    11101 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    11161 tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc gtgcggctcc
    11221 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11281 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11341 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11401 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11461 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11521 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11581 actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    11641 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    11701 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    11761 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    11821 ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11881 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11941 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    12001 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    12061 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    12121 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
    12181 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    12241 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12301 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12361 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    12421 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12481 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    12541 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    12601 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12661 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12721 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12781 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12841 gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    12901 caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt catcgccact
    12961 ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac gatattttcc
    13021 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    13081 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    13141 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13201 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccagcagc
    13261 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13321 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    13381 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    13441 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13501 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    13561 ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga gaatcaatag
    13621 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    13681 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13741 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    13801 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13861 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    13921 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13981 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    14041 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    14101 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    14161 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    14221 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14281 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14341 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    14401 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14461 taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta agccttcttt
    14521 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
    14581 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14641 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14701 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    14761 gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    14821 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14881 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    14941 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    15001 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    15061 aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca gcaccatgtg
    15121 atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    15181 ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg taacgttgtg
    15241 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15301 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15361 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15421 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    15481 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15541 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15601 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15661 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
    15721 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
    15781 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
    15841 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
    15901 taatttactc atggcaggta accagttcag aagctgctat cagacactct ttttttaatc
    15961 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
    16021 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
    16081 gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc
    16141 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
    16201 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
    16261 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
    16321 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
    16381 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
    16441 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
    16501 gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa taacatcacc
    16561 agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg
    16621 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
    16681 ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga ccaaataacc
    16741 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
    16801 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
    16861 aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
    16921 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
    16981 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
    17041 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
    17101 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
    17161 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
    17221 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
    17281 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
    17341 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
    17401 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
    17461 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
    17521 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
    17581 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
    17641 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
    17701 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
    17761 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
    17821 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
    17881 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
    17941 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
    18001 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
    18061 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
    18121 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
    18181 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
    18241 ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg agcagtcccc
    18301 acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt
    18361 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
    18421 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
    18481 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
    18541 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
    18601 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt
    18661 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
    18721 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
    18781 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
    18841 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
    18901 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
    18961 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
    19021 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
    19081 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
    19141 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
    19201 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
    19261 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acaagttttt
    19321 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
    19381 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
    19441 gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt gcaatggcag
    19501 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
    19561 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
    19621 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
    19681 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
    19741 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
    19801 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
    19861 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
    19921 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
    19981 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
    20041 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
    20101 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
    20161 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
    20221 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
    20281 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
    20341 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
    20401 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    20461 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    20521 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
    20581 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    20641 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
    20701 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgagcca
    20761 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    20821 ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    20881 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    20941 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    21001 aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    21061 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
    21121 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    21181 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    21241 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    21301 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    21361 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    21421 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa
    21481 accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    21541 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    21601 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    21661 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    21721 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    21781 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    21841 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
    21901 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
    21961 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    22021 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    22081 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    22141 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    22201 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    22261 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    22321 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    22381 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    22441 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    22501 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    22561 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    22621 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    22681 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    22741 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    22801 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    22861 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    22921 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
    22981 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    23041 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
    23101 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
    23161 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
    23221 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
    23281 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    23341 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    23401 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    23461 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    23521 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    23581 atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    23641 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    23701 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    23761 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    23821 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    23881 taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg accaccaatc
    23941 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    24001 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
    24061 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    24121 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    24181 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
    24241 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
    24301 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    24361 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
    24421 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    24481 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    24541 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    24601 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
    24661 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    24721 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    24781 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    24841 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    24901 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    24961 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
    25021 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    25081 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    25141 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    25201 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    25261 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    25321 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    25381 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
    25441 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
    25501 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
    25561 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    25621 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    25681 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
    25741 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    25801 gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    25861 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
    25921 gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct
    25981 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    26041 gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt gcattttcag
    26101 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
    26161 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    26221 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    26281 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
    26341 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    26401 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    26461 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    26521 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    26581 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    26641 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    26701 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    26761 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    26821 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    26881 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    26941 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    27001 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    27061 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    27121 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    27181 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    27241 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    27301 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    27361 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    27421 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    27481 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    27541 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    27601 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    27661 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    27721 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    27781 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    27841 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    27901 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    27961 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    28021 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    28081 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    28141 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    28201 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt
    28261 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    28321 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    28381 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    28441 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    28501 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    28561 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    28621 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    28681 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    28741 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    28801 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    28861 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    28921 tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    28981 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    29041 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    29101 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    29161 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    29221 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    29281 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    29341 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    29401 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    29461 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    29521 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    29581 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    29641 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    29701 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    29761 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    29821 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    29881 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    29941 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    30001 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    30061 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    30121 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    30181 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    30241 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    30301 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    30361 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    30421 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    30481 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    30541 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    30601 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    30661 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    30721 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    30781 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    30841 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    30901 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    30961 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    31021 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    31081 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    31141 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    31201 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    31261 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    31321 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    31381 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    31441 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    31501 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    31561 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    31621 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    31681 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    31741 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    31801 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    31861 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    31921 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    31981 gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    32041 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    32101 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    32161 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    32221 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    32281 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    32341 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    32401 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    32461 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    32521 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    32581 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    32641 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    32701 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    32761 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    32821 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    32881 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    32941 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    33001 atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    33061 gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcccgttca
    33121 ggcgaaagcg tcgaagtgcg cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat
    33181 tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc
    33241 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    33301 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    33361 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    33421 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    33481 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    33541 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    33601 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    33661 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    33721 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    33781 atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
    33841 ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc
    33901 tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa tgccgacctt
    33961 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    34021 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    34081 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    34141 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    34201 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    34261 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    34321 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    34381 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    34441 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    34501 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    34561 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    34621 taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    34681 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag aaatttatgg
    34741 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    34801 ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat aatagtaaat
    34861 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc ccccactccg
    34921 gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    34981 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    35041 tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt cgcgccaaat
    35101 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    35161 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    35221 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    35281 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    35341 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    35401 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    35461 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttgtaa
    35521 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    35581 gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    35641 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc aacatgtttt
    35701 caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    35761 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    35821 atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc cgtaaaccag
    35881 taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac aaccgtggtc
    35941 agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg gcagacggcg
    36001 gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc cccaggaaga
    36061 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    36121 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    36181 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    36241 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    36301 acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac gaccgatgtt
    36361 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccag
    36421 gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg tatagaaaat
    36481 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    36541 gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat ccagcagctc
    36601 ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa ccgggacacc
    36661 ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    36721 gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc
    36781 ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg gacctttacg
    36841 accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg tgtcagcccc
    36901 gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    36961 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa ttatcagatc
    37021 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    37081 ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt
    37141 cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac gcgccatatc
    37201 agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc cgcccattgc
    37261 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    37321 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    37381 ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    37441 cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat
    37501 aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac gatactcacc
    37561 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    37621 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    37681 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    37741 tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca gaatagaggc
    37801 ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt
    37861 atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt ctttaataat
    37921 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    37981 gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat agccgccgca
    38041 ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac cagtggaatg
    38101 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    38161 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    38221 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc cgttgataaa
    38281 gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa taatttcttg
    38341 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    38401 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    38461 aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt ggcccagcgc
    38521 tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg catctgccca
    38581 gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    38641 cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac ggatcacttc
    38701 catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg tagaggtttt
    38761 gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    38821 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    38881 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcgcggta
    38941 cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg tttctgccag
    39001 cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga ttcggatttc
    39061 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    39121 gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa ttactttctc
    39181 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    39241 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    39301 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    39361 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    39421 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    39481 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    39541 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    39601 attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    39661 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    39721 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa tggctggagc
    39781 tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt taccaccacg
    39841 taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg ttgcaaccat
    39901 atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc tggcgttacc
    39961 tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct gattcgccca
    40021 ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg aacctgacct
    40081 ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct
    40141 gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag
    40201 ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc
    40261 tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta
    40321 cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat
    40381 tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta
    40441 accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc
    40501 tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca aatgaagtgg
    40561 gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct
    40621 ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc ggcaccacgc
    40681 cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg caaactgtcg
    40741 ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc gacgaaacca
    40801 tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc
    40861 gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc
    40921 gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg
    40981 gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg
    41041 aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc
    41101 agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg
    41161 tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg
    41221 ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca gtgtgatacc
    41281 tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag cgtggacgaa
    41341 gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt aagcggcggc
    41401 gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg
    41461 caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga aaccggctgg
    41521 gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg ggggagcgaa
    41581 tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat ctgtttgctt
    41641 gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca ggtggattat
    41701 ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt tccggttcgc
    41761 ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg
    41821 ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata
    41881 tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag
    41941 gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc ggatgcggcg
    42001 tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcag
    42061 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    42121 cggttcggca cagacttgta ggcatgataa gacgcgtcaa gcgtcgcatc aggcattgtg
    42181 cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaaccagt
    42241 aggcatgata agacgcgtca agcgtcgcat caggcattgt gcgccaactg ccggatgcgg
    42301 cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat aagacgcgtt
    42361 aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag ggtaggctac
    42421 ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat ctcatcttct
    42481 ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc cggtcggtta
    42541 ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag caacaacgat
    42601 gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata ggtgatagta
    42661 ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg caggctgccg
    42721 gaaaaaccgt gaaccacgcc agtgcacggt aaatcgtgac gtttaagatg catcgccagt
    42781 ttgtcgtgta tacgccggga atgaaggatc accggcagat cgtagcgttt cgccagtttc
    42841 agctgttcgt cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc gaagagatcc
    42901 agaccgatct cccccaccgc caccaccttc gccggacgcc tttccagcgc ctgctgtagc
    42961 tgatcaagag acacatcgct atgtttttcc aacatccccg gatgcaaccc caatgcggca
    43021 tacagcggtt gatatttttc cgctaacgcc tgcacgcggg caaaattttc cgcctctgtc
    43081 gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct ggcctcttca
    43141 tcgccactaa acggcgggaa atcaaaatgg cagtgggtat cgataaaacg gcaaatcaag
    43201 ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcatcgctc acttcggcaa
    43261 ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc gtcaccactg
    43321 gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc gtggcgagga
    43381 aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga atccgactgc
    43441 caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc agcttgcccg
    43501 gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc tgcaccagat
    43561 tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg aaccactgcg
    43621 gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta gcgccctgcc
    43681 ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag ttaatgcctt
    43741 ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga atcacatcca
    43801 gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga cgacaggcgc
    43861 acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt tgcaacggca
    43921 tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct ccacggacaa
    43981 agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact ttgcgcgcgt
    44041 aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat gtgccgagat
    44101 aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca gccgtgaaaa
    44161 ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg aacggcctta
    44221 gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc tctgccttcc
    44281 tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa aaccgcaaaa
    44341 gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat ctagggtcgt
    44401 tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa gacaatttta
    44461 gctgcgcctg cagcggtacc ggccagacca ccaaacccaa gtgcggcggc gattaacgcg
    44521 ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat agttaatgtc
    44581 accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg tgcacatcgc
    44641 gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt cagatcattt
    44701 ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc gctctgtgcc
    44761 tgggaatcga cggtaccgga gagctgaacc acgccgtcgg tggtttcaac tttcacatga
    44821 cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt ggtggcggtg
    44881 tcacccgcgt agcccttcac agagccttct ttagcgtcgc gaacgtgcag tttgtcgctg
    44941 acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc ctgagcctgg
    45001 ctttcaacga aaccgctcag ggtcacgact ttttgatcgg ttttgacaga gatatcggtg
    45061 ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat ggcgctgtca
    45121 tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc gctttcattg
    45181 gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga ggtcaacatt
    45241 acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt attcctgtat
    45301 gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg gagagacttc
    45361 accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa taacgaccat
    45421 tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct ttagatactg
    45481 atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt gttaaatata
    45541 gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag tgaatgtttt
    45601 aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag cggctaccgc
    45661 gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg ggataacgtt
    45721 cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta tcgccgccat
    45781 caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc gcgtccgcac
    45841 attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca acgctgtatt
    45901 cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca ccaacgatca
    45961 aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa agctgtacca
    46021 gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga cggaacagtt
    46081 ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta aaggtgtcgc
    46141 cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac gcttcttcaa
    46201 cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca tctttcgcag
    46261 tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg cgcataaacg
    46321 ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat tttaaatacg aagccggtaa
    46381 atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc ggcgcaggag
    46441 cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc gcagtaccga
    46501 agaataccgg agtgatttcg cccgcaagga acaattcttt gtcgaactcg ttagacgcgc
    46561 ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca accgcagcgt
    46621 cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg tggcctttac
    46681 cgctctgata gagataggtt tcgtctttat aaaggtggta aacgccttta aacagcttgc
    46741 cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc tcaacttcat
    46801 cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag gtgaggatcg
    46861 gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca acaccttttg
    46921 cagcgtcgat aaccatcagg cagcagtcca ccgccgtcag ggtacgatag gtatcttccg
    46981 agaagtcttc gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga tacggaaact
    47041 gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc cagtccgact
    47101 tagcgtgctg gttggaacca cggcctttta ctgtaccggc ggtctgaatg gcctgtccga
    47161 acagcagcac cttctcggtg atggtagtct taccggcatc cgggtgagaa ataatggcaa
    47221 aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt ctttcttctt
    47281 gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat tttacccatc
    47341 aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc aacgaagaaa
    47401 cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt gtgcgcattc agccagcagg
    47461 tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg ccaaccatca
    47521 gcacgcgtga acggtcagga ttgcccgcct gttccagcgc ataatcgaaa attttcttat
    47581 tcggtttggc aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa tcacgcaggc
    47641 ccgtgcgttc cagacgcact tgctgcaagg cgctaaagcc gttggtgatg atgccgattt
    47701 tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc gtgcagattt
    47761 ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc agccgttcgg
    47821 cccagctctc aaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg ttttgataat
    47881 ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg gtaaaggtga
    47941 cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta aacagcgttt
    48001 catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt acatactgat
    48061 tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt aattgcggcg
    48121 aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag catttgaggc
    48181 acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga tcagatgttc
    48241 cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccgcaa tattgaacaa
    48301 tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc cgttttgcgt
    48361 taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt cactccacgg
    48421 aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg tttcgaggga
    48481 agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc gttgggtttg
    48541 cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat agcggttcgt
    48601 cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg attttttctg
    48661 gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca tcgctcacta
    48721 aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg tgtgccggga
    48781 tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac tgggtaatgc
    48841 ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc tgttgtcagg
    48901 ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat cgccgccagt
    48961 atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct tgctgcgtca
    49021 cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg atgacctgcc
    49081 cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc actggcaggt
    49141 attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg tggatgacgt
    49201 cgcagattgc gatatgctga tttactactg gccgaagaac aaaccggaag cccagttcca
    49261 gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg ttggcgagaa
    49321 ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt tgaataaagt
    49381 cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc cggtatttga
    49441 tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa cgctgcctgg
    49501 cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga cgttaactcc
    49561 gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt cagttgcctt
    49621 tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc cagcggtaga
    49681 agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg ccagcaacgt
    49741 cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt tccacgatgg
    49801 gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc gtcatcttaa
    49861 tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttacccgg acgtgctgga
    49921 tgagacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg tgtatcgcgc
    49981 cattatgacc cgccaggcga agaaaggtta attatctcgc cgaataccgt gtcggatgcg
    50041 gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag gccagataag
    50101 acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg cacgctagtt
    50161 tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat tgacgaaaag
    50221 ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt gatggt
//
LOCUS       DAEEBB010000010        49699 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEEBB010000010 DAEEBB010000000
VERSION     DAEEBB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.010"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..455
                     /locus_tag="KLF07_000595"
     CDS             <1..455
                     /locus_tag="KLF07_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD9830708.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="KLF07_000596"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="KLF07_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBD9830709.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="KLF07_000597"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="KLF07_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBD9830710.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="KLF07_000598"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="KLF07_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBD9830711.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="KLF07_000599"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="KLF07_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBD9830712.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="KLF07_000600"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="KLF07_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD9830713.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="KLF07_000601"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="KLF07_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBD9830714.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="KLF07_000602"
     CDS             complement(6424..6918)
                     /locus_tag="KLF07_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBD9830715.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="KLF07_000603"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="KLF07_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBD9830716.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="KLF07_000604"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="KLF07_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBD9830717.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="KLF07_000605"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="KLF07_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBD9830718.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="KLF07_000606"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="KLF07_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBD9830719.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="KLF07_000607"
     CDS             complement(12082..12369)
                     /locus_tag="KLF07_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBD9830720.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="KLF07_000608"
     CDS             complement(12488..13375)
                     /locus_tag="KLF07_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD9830721.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="KLF07_000609"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="KLF07_000609"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBD9830722.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="KLF07_000610"
     CDS             complement(14612..15106)
                     /locus_tag="KLF07_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBD9830723.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="KLF07_000611"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="KLF07_000611"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD9830724.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="KLF07_000612"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="KLF07_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBD9830725.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="KLF07_000613"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="KLF07_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD9830726.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="KLF07_000614"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="KLF07_000614"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBD9830727.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="KLF07_000615"
     CDS             19086..19745
                     /locus_tag="KLF07_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD9830728.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="KLF07_000616"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="KLF07_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBD9830729.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="KLF07_000617"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="KLF07_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBD9830730.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="KLF07_000618"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="KLF07_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBD9830731.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="KLF07_000619"
     CDS             23173..24099
                     /locus_tag="KLF07_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBD9830732.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="KLF07_000620"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="KLF07_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBD9830733.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="KLF07_000621"
     CDS             complement(24450..26669)
                     /locus_tag="KLF07_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBD9830734.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="KLF07_000622"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="KLF07_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBD9830735.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="KLF07_000623"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="KLF07_000623"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBD9830736.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="KLF07_000624"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="KLF07_000624"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBD9830737.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="KLF07_000625"
     CDS             complement(32041..32523)
                     /locus_tag="KLF07_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9830738.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="KLF07_000626"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="KLF07_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBD9830739.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="KLF07_000627"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="KLF07_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBD9830740.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="KLF07_000628"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="KLF07_000628"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBD9830741.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="KLF07_000629"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="KLF07_000629"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBD9830742.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="KLF07_000630"
     CDS             35847..36161
                     /locus_tag="KLF07_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBD9830743.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="KLF07_000631"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="KLF07_000631"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBD9830744.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="KLF07_000632"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="KLF07_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBD9830745.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="KLF07_000633"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="KLF07_000633"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBD9830746.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="KLF07_000634"
     CDS             complement(39563..39985)
                     /locus_tag="KLF07_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBD9830747.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="KLF07_000635"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="KLF07_000635"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBD9830748.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="KLF07_000636"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="KLF07_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBD9830749.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="KLF07_000637"
     CDS             42449..43120
                     /locus_tag="KLF07_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBD9830750.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="KLF07_000638"
     CDS             43126..44286
                     /locus_tag="KLF07_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD9830751.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="KLF07_000639"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="KLF07_000639"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBD9830752.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="KLF07_000640"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="KLF07_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBD9830753.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="KLF07_000641"
     CDS             complement(46086..46256)
                     /locus_tag="KLF07_000641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830754.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="KLF07_000642"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="KLF07_000642"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBD9830755.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="KLF07_000643"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="KLF07_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBD9830756.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="KLF07_000644"
     CDS             48117..48668
                     /locus_tag="KLF07_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBD9830757.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..>49699
                     /locus_tag="KLF07_000645"
     CDS             48728..>49699
                     /locus_tag="KLF07_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD9830758.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT        12447 a        13186 c        12329 g        11737 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 gacggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg actttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgta tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg ctgaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacag
//
LOCUS       DAEEBB010000011        48840 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEEBB010000011 DAEEBB010000000
VERSION     DAEEBB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.011"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            34..663
                     /locus_tag="KLF07_000646"
     CDS             34..663
                     /locus_tag="KLF07_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBD9830759.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="KLF07_000647"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="KLF07_000647"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBD9830760.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="KLF07_000648"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="KLF07_000648"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBD9830761.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="KLF07_000649"
     CDS             complement(3031..3228)
                     /locus_tag="KLF07_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD9830762.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="KLF07_000650"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="KLF07_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBD9830763.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="KLF07_000651"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="KLF07_000651"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBD9830764.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="KLF07_000652"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="KLF07_000652"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBD9830765.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="KLF07_000653"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="KLF07_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBD9830766.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="KLF07_000654"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="KLF07_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBD9830767.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="KLF07_000655"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="KLF07_000655"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBD9830768.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="KLF07_000656"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="KLF07_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBD9830769.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="KLF07_000657"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="KLF07_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBD9830770.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="KLF07_000658"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="KLF07_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBD9830771.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="KLF07_000659"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="KLF07_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBD9830772.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="KLF07_000660"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="KLF07_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD9830773.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="KLF07_000661"
     CDS             complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="KLF07_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBD9830774.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17457..21338)
                     /gene="entF"
                     /locus_tag="KLF07_000662"
     CDS             complement(17457..21338)
                     /gene="entF"
                     /locus_tag="KLF07_000662"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBD9830775.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="KLF07_000663"
     CDS             complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="KLF07_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBD9830776.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21556..22758)
                     /gene="fes"
                     /locus_tag="KLF07_000664"
     CDS             complement(21556..22758)
                     /gene="fes"
                     /locus_tag="KLF07_000664"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBD9830777.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23001..25241
                     /gene="fepA"
                     /locus_tag="KLF07_000665"
     CDS             23001..25241
                     /gene="fepA"
                     /locus_tag="KLF07_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBD9830778.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEINPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25416..26036
                     /gene="entD"
                     /locus_tag="KLF07_000666"
     CDS             25416..26036
                     /gene="entD"
                     /locus_tag="KLF07_000666"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBD9830779.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="KLF07_000667"
     CDS             complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="KLF07_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBD9830780.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="KLF07_000668"
     CDS             complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="KLF07_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBD9830781.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27253..28371
                     /locus_tag="KLF07_000669"
     CDS             27253..28371
                     /locus_tag="KLF07_000669"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD9830782.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            28437..28685
                     /locus_tag="KLF07_000670"
                     /pseudo
     CDS             28437..28685
                     /locus_tag="KLF07_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            28750..29118
                     /locus_tag="KLF07_000671"
     CDS             28750..29118
                     /locus_tag="KLF07_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD9830783.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29212..29865
                     /gene="nfsB"
                     /locus_tag="KLF07_000672"
     CDS             29212..29865
                     /gene="nfsB"
                     /locus_tag="KLF07_000672"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBD9830784.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            29973..31220
                     /gene="ybdG"
                     /locus_tag="KLF07_000673"
     CDS             29973..31220
                     /gene="ybdG"
                     /locus_tag="KLF07_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBD9830785.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="KLF07_000674"
     CDS             complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="KLF07_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBD9830786.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="KLF07_000675"
     CDS             complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="KLF07_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBD9830787.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="KLF07_000676"
     CDS             complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="KLF07_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBD9830788.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="KLF07_000677"
     CDS             complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="KLF07_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBD9830789.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="KLF07_000678"
     CDS             complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="KLF07_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBD9830790.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            39055..39738
                     /gene="cusR"
                     /locus_tag="KLF07_000679"
     CDS             39055..39738
                     /gene="cusR"
                     /locus_tag="KLF07_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBD9830791.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            39728..41176
                     /gene="cusS"
                     /locus_tag="KLF07_000680"
     CDS             39728..41176
                     /gene="cusS"
                     /locus_tag="KLF07_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBD9830792.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            41913..43814
                     /locus_tag="KLF07_000681"
     CDS             41913..43814
                     /locus_tag="KLF07_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBD9830793.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            43842..44303
                     /locus_tag="KLF07_000682"
     CDS             43842..44303
                     /locus_tag="KLF07_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBD9830794.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            44323..48734
                     /locus_tag="KLF07_000683"
                     /pseudo
     CDS             44323..48734
                     /locus_tag="KLF07_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        11692 a        13454 c        12816 g        10878 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tccactgaat gcaatattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    17401 caaataaaaa acccataatt attttctgta attatgggtt tatagataat attaatttac
    17461 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
    17521 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
    17581 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
    17641 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
    17701 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
    17761 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
    17821 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
    17881 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
    17941 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
    18001 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
    18061 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
    18121 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
    18181 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
    18241 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
    18301 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
    18361 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
    18421 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
    18481 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
    18541 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
    18601 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
    18661 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
    18721 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
    18781 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
    18841 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
    18901 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
    18961 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
    19021 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
    19081 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
    19141 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
    19201 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
    19261 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
    19321 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
    19381 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
    19441 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
    19501 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
    19561 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
    19621 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
    19681 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
    19741 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
    19801 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
    19861 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
    19921 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
    19981 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
    20041 atatcgacat cgccgcacaa cagcgccgga tccgcggcga actgggcaat cagcattttc
    20101 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
    20161 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
    20221 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
    20281 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
    20341 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
    20401 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
    20461 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
    20521 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
    20581 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
    20641 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
    20701 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
    20761 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
    20821 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
    20881 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
    20941 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
    21001 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
    21061 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
    21121 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
    21181 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
    21241 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
    21301 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
    21361 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
    21421 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
    21481 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
    21541 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
    21601 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
    21661 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
    21721 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
    21781 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
    21841 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
    21901 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
    21961 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
    22021 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
    22081 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
    22141 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
    22201 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
    22261 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
    22321 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
    22381 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
    22441 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
    22501 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
    22561 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
    22621 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
    22681 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
    22741 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
    22801 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
    22861 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
    22921 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
    22981 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
    23041 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
    23101 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
    23161 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
    23221 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
    23281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
    23341 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
    23401 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
    23461 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
    23521 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
    23581 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
    23641 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
    23701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
    23761 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
    23821 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
    23881 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
    23941 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
    24001 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
    24061 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
    24121 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
    24181 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
    24241 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
    24301 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
    24361 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
    24421 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
    24481 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
    24541 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
    24601 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
    24661 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
    24721 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
    24781 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
    24841 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
    24901 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
    24961 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
    25021 atccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
    25081 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
    25141 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
    25201 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
    25261 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
    25321 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
    25381 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
    25441 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
    25501 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
    25561 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
    25621 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
    25681 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
    25741 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
    25801 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
    25861 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
    25921 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
    25981 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
    26041 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
    26101 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
    26161 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
    26221 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    26281 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    26341 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
    26401 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    26461 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
    26521 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
    26581 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
    26641 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
    26701 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
    26761 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
    26821 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
    26881 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa
    26941 ccggaaagct tcaggcaccc gccgttatct tacccttcct caacaaaatg gctgtttatt
    27001 tatacagtaa atcctgtttc ggacttctca ctcctcattt aatcaactga agctgagccc
    27061 gttttttctg catgcctcaa tcgtcaaaat tcttcgccat cagccagctc tgccataact
    27121 tgccacattg aaaaagtagc caaattgctt cagtcaccat catcaaaaaa agaaacattc
    27181 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
    27241 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
    27301 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
    27361 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
    27421 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    27481 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
    27541 ggtggcacac acccgtttca gaaatggcag cgtcaggagg tatgcgataa cgagcgctat
    27601 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    27661 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    27721 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    27781 cgttttgcct cctcacgacc gaatattttt tccgcctttc ccgataatgg cccgatgccg
    27841 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtttgagtta caccacgatg
    27901 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
    27961 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    28021 attcaggcta ccgcccactg gttactgaca gaacgcccgt tcaaacatca ggagaaagat
    28081 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    28141 accgatccgc acactggcga tcgtcgaccg ctaacggaag acaccttgcg attgctggaa
    28201 aaaatcgccc cttccgcaca taaaattggt gcatcgagcg cgattgaggc cctgcatcgc
    28261 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc
    28321 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccgatgacta agtagggaat
    28381 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    28441 aacacccttt agaaaccttg actaccgcag caggcatttt gctgatggct ttcctctctt
    28501 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
    28561 tccatctgat ggatcttagc tagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    28621 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
    28681 attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc ttcgcctaaa
    28741 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc ccttgagtta
    28801 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    28861 aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    28921 ccacaaaaat ccctgttaaa tcagcagata tacccgagca ttaagccagg gtatcacatg
    28981 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    29041 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    29101 agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta ccatttctcg
    29161 ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    29221 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa aaaacttacc
    29281 ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag caccaactcc
    29341 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    29401 gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg
    29461 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    29521 gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    29581 ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    29641 tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga cgcggtaccc
    29701 atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga gaaaggctac
    29761 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
    29821 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    29881 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    29941 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    30001 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
    30061 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    30121 aacgagccat cgccagttca cggctttggt tgcaaatcat aacccagaat aaactcttcc
    30181 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    30241 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    30301 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    30361 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    30421 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    30481 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    30541 tggtggccgg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    30601 tgccgaaata cggcgcagat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    30661 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    30721 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    30781 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    30841 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    30901 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    30961 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
    31021 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
    31081 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    31141 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    31201 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
    31261 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
    31321 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
    31381 catcagcagc aaaatcatgc cgaggaaggc aatgcagaga tagtttccga acggatagag
    31441 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg
    31501 cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    31561 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    31621 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
    31681 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    31741 atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    31801 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    31861 atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc gatacaccac
    31921 ctgatttacc gcttttggaa tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc
    31981 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
    32041 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
    32101 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga ttgccagcac
    32161 tttaatcaac gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    32221 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    32281 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    32341 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    32401 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
    32461 gaaagcgatg atcccggcga cgccgtaacc cagcaataca gccggacccg ccatctgaat
    32521 cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    32581 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    32641 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    32701 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    32761 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    32821 ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg
    32881 ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc ggcgataatc
    32941 accgccaccg tcatcgcttt cgggcgcacg cgcagtaccg cgccgtgata caacgcctca
    33001 tccagcttct gctcactgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    33061 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    33121 aaaccggtgc ccgttgccac agaaagatga aagcccatcc accacagcaa ccagatgccg
    33181 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaat
    33241 gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag tttcagctta
    33301 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    33361 aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac catgtcacga
    33421 tcgcgggcat caatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    33481 ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg cttcatcggc
    33541 gtcaggatcg gtagctggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    33601 agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    33661 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    33721 cggttaatct caacgttgat atagcgccca ccttccagac gctcggctag agcagaagcc
    33781 acgcttggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc gatatccgcc
    33841 agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga gagcatatcg
    33901 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg cacggtgtta
    33961 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    34021 agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt ttccgctttc
    34081 ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt gtcggttttt
    34141 tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    34201 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    34261 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    34321 agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    34381 ggaattttgc cacggatcca gtaccccatc aggataggga tcactacgat cgccagcagc
    34441 gccgcgcccg ccatcgcata ggttttagtg aacgccaacg ggccaaacag acgcccttcc
    34501 tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag actgataaat
    34561 agcgccggac ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    34621 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgtgc attttcgatc
    34681 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccgcc caacgacata
    34741 atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa ccccagcggc
    34801 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    34861 gccaccacga taaactcttc cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg
    34921 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    34981 gtttccagtt tgtccttcac ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg
    35041 atcaccaccc cgcccgccac ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc
    35101 tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc attttctctg
    35161 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    35221 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    35281 agcgcgcttt ttacttcggc tagactgatg ccatattgcg ccaggcgctg gggatcgata
    35341 accacctgat actctttcac cacgccgccc accgacgcca cttccgcaac gtcagggatg
    35401 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    35461 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
    35521 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    35581 acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata cacataagag
    35641 tctccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    35701 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    35761 ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt gttaatgatg
    35821 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
    35881 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca ttggtagcac
    35941 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
    36001 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt
    36061 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa
    36121 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    36181 cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    36241 tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg
    36301 tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg
    36361 aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc cacaccgggt
    36421 ccataccctg aattttcgct accacgttat ctttggcgat attcattccc gcgcgcagat
    36481 caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt
    36541 gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc agccgctcaa
    36601 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    36661 gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt tgtactttat
    36721 cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc tgcacaatgg
    36781 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc
    36841 cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    36901 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    36961 gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac cagaataaga
    37021 ttttacgttc tgcggtcgac gttttttctg cgggtggttc agcctttgca aaccaggtaa
    37081 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    37141 tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    37201 cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc attttcgtct
    37261 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    37321 gatggatggt gatttttttg ctttccagat caataccctt taccacgcca gtggcgctaa
    37381 taacctgcgg ttgtacttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    37441 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    37501 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtataca aagaaatttc
    37561 gttaacctga cgggcataat tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg
    37621 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    37681 ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgttcag
    37741 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    37801 ataattcact accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    37861 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc
    37921 gttaaataat gaagacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    37981 cgggaaaaat gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc
    38041 agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    38101 aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc
    38161 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    38221 actgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    38281 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    38341 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    38401 aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc
    38461 ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc
    38521 ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc agctgccgct
    38581 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc
    38641 ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat
    38701 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc
    38761 tgctgcgtta accaagccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgt
    38821 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    38881 aagtttacaa ggagacatag gctcataatt tctggtgatt ttataccgcc aactttactc
    38941 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    39001 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa
    39061 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    39121 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    39181 ggtgattatg acctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    39241 gtgcgcatgt tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc
    39301 accattgaac atcgcgtcaa ggggctggag ttgggggccg atgactatct ggtgaagccg
    39361 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg
    39421 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    39481 acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct ggagtttttt
    39541 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg
    39601 aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc
    39661 gacaacgact ttgagccgaa gctgattcag accgtgcgcg gcgtgggcta tatgcttgag
    39721 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gtctgacctt
    39781 ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    39841 agtgaaagtg cattttgccg agcaggatat taatgattta aaagaaatta gcgccaccct
    39901 tgaacgggta cttaatcatc ctgacgagac gcaagcccga cgcttaatga cgctggaaga
    39961 tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt
    40021 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    40081 agacgctcag ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca
    40141 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt
    40201 ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    40261 ttacataaat gatttgatga ataaacttat tatgaccgca tctgtcatca gcatcctgat
    40321 cgtctttatc gtgctattgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    40381 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgagcc
    40441 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    40501 ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca cgccgattac
    40561 caatctcatt acgcaaacgg aaatcgccct cagccagtct cgcagccaga aggagctgga
    40621 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    40681 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    40741 ggcggatgaa gtcggcaaag tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt
    40801 ggagttgcga tttgttggcg acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg
    40861 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccagag agacaattgt
    40921 agtgcgctgt cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc
    40981 cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg acccttcccg
    41041 ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc
    41101 gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg tgattatgct
    41161 gccggagaga gagtgattat taagttcaaa ttcagggcgt ggtgcatcac ctcctggtat
    41221 tgttgatcaa caaattagag aaaaacttat taaacatatg aaatatcgaa ggcttttatt
    41281 tttcaaagtt tcacgagatg tttgtatatg aaacttagtt aaatctgtgt ttcttccaat
    41341 tgtagatcac aatgcccttt ctaaattttc atcgttataa acaagccatc tatcatataa
    41401 agcaatgaat aatttaatcg cgattaatca tcattaattg ttatattatt cactcaatct
    41461 atttgtcaag tgaatttgat aaattcctaa tttaaaatat ccactattga gccagtattt
    41521 aacaacgtta agatgtgcaa tgtgcagatc atgttatcag ggtggagcgg tagcatatat
    41581 ttcttatgca atcattcatt ttaataatgt taattggatt atttattgct gtgaataaaa
    41641 atatattatg aatattggtt taactttgcg tttttgttac tcgttgtgat atgcgtacct
    41701 gtcctgaata acgccaagaa acaagaggtt gagcttgtga ttattttttt gactgaacag
    41761 acacccgttt ctgtaaagta atatttaaac aaaattctca cataaccata gcattttctg
    41821 ttccttccct aataacgtct gaagtcgtcc cgcttatcac attatccgca gttgtacggt
    41881 acggatcatt attcatttta ctggaggttg ctatgtcaac cggattacgt ttcacactgg
    41941 aagtggacgg cctgccgccg gacgcttttg cggtggtttc ctttcatctg aaccagtcac
    42001 tctcttcgct cttttccctc gatctctccc tggtcagcca gcagtttctc tcccttgaat
    42061 ttgcgcaggt gctggacaaa atggcctacc tgacgatatg gcagggcgat gaagtacagc
    42121 gccgggtgaa aggcgtggtg acctggtttg aactcgggga gaacgacaaa aaccagatgc
    42181 tgtacagcat gaaggtgcac ccgccgctgt ggcgtgccgg tctgcgccag aacttccgta
    42241 tcttccagaa cgaggacatc aaaagcatcc tcggcacgat gttgcaggaa aacggggtga
    42301 ccgaatggag tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg
    42361 gtgagactga ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg
    42421 aggagcatgc ttacaaaagt accgaccaga gcctggtgct gtgcgacaca gtccgccatc
    42481 tgcccgaatc ttttgaaatc ccatggaacc cgaacacccg taccgaggtg agcaccctct
    42541 gcatcagcca gttccgctac agcgcacaaa tccgcccttc ttccgtggtg accaaagact
    42601 acacctttaa acgccccggc tgggccggac gttttgaaca ggaaggccag caccaggact
    42661 accagcgcac gcagtatgaa gtgtatgact accccggacg tttcaagggt gctcacgggc
    42721 agaactttgc ccgctggcag atggacggct ggcgaaacaa tgcagaaacc gcgcggggaa
    42781 tgagtcgctc gccggagata tggccgggac gacgaattgt gctgacgggg catccgcagg
    42841 cgaacctgaa ccgggaatgg caggtggtgg caagtgaact gcacggcgaa cagccacagg
    42901 cggtgccagg acggcaggga gcggggacgg cgctggagaa ccattttgcg gtgatcccgg
    42961 cagacagaac atggcgacca cagccgttgc tgaaaccgct ggtcgacggc ccgcagagcg
    43021 ctgtcgtgac aggaccggca ggcgaggaaa tcttctgcga cgaacatggt cgcgtgcggg
    43081 tgaagttcaa ctgggaccgt tataacccgg cagaccaaga cagttcgtgc tggatccgtg
    43141 tggcacaggc gtgggcaggc accggttttg gccacctggc gataccgcgt gtgggtcagg
    43201 aagtgattgt agacttcctc aacggcgatc cggaccagcc gatcattatg gggcgcacct
    43261 accaccagga aaaccgcccc cccggcagcc tgccgggaac aaagacgcag atgaccatcc
    43321 gctccaaaac gtacatgggc agcggattta atgagctgaa gtttgatgat gcgacaggga
    43381 gagaacaggt ctacatccac gcgcagaaga acatggatac cgaagtgctc aacgaccgta
    43441 ccaccaccgt aaaacacgat caccgcgaaa ccgtaaaaaa tgaccagacg gtcacgatcc
    43501 aggaaggtaa ccgccttctt acggtggaaa aaggccacaa gatcaccgga gtactgaaag
    43561 ggtctttatc tgaggatgtc tttcaggaca gaggcacgat tgccggttcg gtgcatgttg
    43621 acgctgtaaa caatggtggc gaaggcgacg gtatacaggc ttatacggcg attaaggaaa
    43681 ttttgctggc cgtggaggaa agcaaaattg cgctgacgcc ggatagcatt cagctacagg
    43741 tcggggaatc gacggtaatc aggctgtcga aggatggcat caccatcgtg ggcggttctg
    43801 ttttcatcaa ctgattcact cagattatca gggagtattt tatgcagttc acttttaatg
    43861 aaggccacat tcaactgccg tctcaatggc aggatcagtc aatgcaggtc ctggtgtcca
    43921 cagataacag tggcatcaat ctggtcatta cccgcgaagc cgttccacag ggaaccctga
    43981 caccagagct gtaccaggaa acgctggcgc tgtaccaggg caaacttgat gggtacaccg
    44041 aacatgcctg ccgggaaatc actctggcag aggccccggc ctggctgctg gattattcct
    44101 ggaacggtcc ggaagatgag gggaatcagg ggcggattag ccagatagcc gtattccagc
    44161 ggcgtggaga tacgctgctg accttcactt tcagcacctc cttatccctg aagaacagtc
    44221 aaaagacgat gctgctggag gtgataaaga gcttcacccc gttgccaccg gagaatgaca
    44281 tccaaaagga ccagccgcgc tgacataaac gagggataac acatgagtga aggaccaggc
    44341 gggccacagg gagcgaccgc aggcggtacg ctggcaatgc gaatgctgtc acagcaggcg
    44401 atggtcgcca gccagatgaa acgggcagcc aacgacaaag ccattgcaca gatgttggca
    44461 tcaaaaaagt ctggcccccc cgccgccagg ctgggcgatg aaattcagca caagagtttt
    44521 ttgggggcgc tggcaggggc cgtactgggg gcgatagtga ccatcgcaga aggttgcctg
    44581 attatggccg cctgtgccac cggcccttat gcgctggttc tggtacctgc gctgatgtat
    44641 gccagctata aggcgagtga ttatgtggag gagaaacaga accagcttga atcatggata
    44701 aacagctttt gtgacacgga cggcgccatc aataccggtt ctgaaaacgt aaaaattaac
    44761 ggcgagctgg ccgcacgtgc agcggtcacc cttccccctc ctcccccacc tggagcaata
    44821 cctgaagtcc cacaggggga accctcatgg ggtgatattg ccactgacct gcttgaatcg
    44881 gcagcggaaa aagcagtacc actggcgaag gcctggggga acgctgttat caccctgacg
    44941 gacagcaatg ccggttttat ggatcgcgtc agcgccggcg catcgcttct gtttcccgcc
    45001 ggtccggtat taatggagtt tgccaccatg gtgggcgggc gtggcgaaat caaaaaagat
    45061 gtggatttcc cggaagccgg tgaggacacg gcgctctgcg acaaggagaa caaaccaccg
    45121 aggatagccc agggcagcag caacgtcttt atcaacaatc agcctgccgc gcgcaagggc
    45181 gacaaactgg agtgcagtgc ggcgatcgtg ggaggttcgc cggacgtctt tattggcggt
    45241 gagcaggtca catatctgga tatccagccg gagttcccgc cgtggcagag aatgatcctg
    45301 gggggaataa cgatagccag ctatcttctg ccgccagcgg gactactggg aaaactgaag
    45361 aatctggcga gactcggcaa actgggaaac tgctggggaa aagcgggaag ctgctgggcg
    45421 caaagctcgg cgcgttgctg gggaaaacag gtaagtcgtt aaaaagtatt gccaataaag
    45481 tcatcagatg ggtaacagat cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta
    45541 ccgacttcac cctgggccag accctccccc tctccttcac ccgtttccac agttctgtac
    45601 tgccgctgca tggcctgacc ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg
    45661 tgcgtgaaca gggaaaccgg gtggatatca tcagccaggg agccacgctg agatttgcct
    45721 tcgacggtga cagtgatacg gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc
    45781 gcgatgatta tctggagctg ttcgacaggg atgcactgag cagccgcttc ttttatgacg
    45841 cctttccggg aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg
    45901 cacacagccc caatgaccgg atgtacatgc tgggcgggat gagcgacacc gccagcaacc
    45961 gcatcacgtt tgagcgcgac agccagtacc ggatcacggg tgtcagtcac accgacggga
    46021 tccggcttaa actgacgtac cacgccagcg gctacctgaa agccattcac cgcacggata
    46081 acgacataca gacgctggcg acctacgaac aggatgcgcg ggggcggctg acagaagcgg
    46141 atgcgcggct ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct
    46201 ggtccgataa cgaccagacg tggagccgtt tcacctacga tgaacagggc cggtgcgtga
    46261 atgtcaccgg ggcggagggc tattacaacg ccacgctgga ctatggtgac ggctgcacca
    46321 ccgtgacgga cggcaagggc attcaccgtt attactatga tcctgacggc aatattctgc
    46381 gggaagaagc gccggacggc agcaccacca tgtatgaatg ggatgaattc catcacctgc
    46441 tggcccgcca ctcccctgcc gggcgggtgg agaaatttga atacaacgcc gcacacggtc
    46501 agttaagccg ttacacggcg gcagacggcg cggagtggca gtaccgctat gatgagcgcg
    46561 gcctgctcag caacatcacc gaccctgccg ggcagacgtg gacacagcag tgtgatgaac
    46621 gcggcctgcc ggtaagtctg gtgtcgccac agggcgaaga gacccggctg gcatacaccg
    46681 ctcaggggct gctgtcggga atattccgcc aggatgaacg gcgtctgggc atagagtacg
    46741 accaccacaa ccggccggaa acactcaccg acgtgatggg ccgtgaacac cacaccgaat
    46801 acagcggtca cgacctgcca gtgaagatgc gcggccccgg cggtcagtca gtgcggttac
    46861 agtggcagca gcaccataaa ctgagcggca ttgaacgtgc tggaaccggc gcggaaggat
    46921 ttcgctatga ccgccacggc aacctgctgg cctggacgga cggcaacggt gttgtctgga
    46981 cgatggaata cggtccgttc gattttccgg tggcgcgaac ggacggtgaa ggccaccgct
    47041 ggcagtaccg ctacgataaa gacacgctgc aactgacaga agtcatcaac ccgcagggtg
    47101 agtcatatct ttatgttctg gataactgcg gccgggtgac ggaagagcgt gactggggcg
    47161 gcgtggtctg gcgttaccgc tatgacgccg atggcctctg taccgccaag gttaacggcc
    47221 tggaggaaac catcctctac agccgggacg ccgcaggccg cctggcagaa atcatctcgc
    47281 cggaaggcaa aacgcagtat gcctatgaca aatccggcag gctgacgggt atcttcagcc
    47341 cggacggcac atcacagcgc accggctatg acgaacgcgg gcgggtgaat gtcaccactc
    47401 agggccgacg ggccattgaa tacaactacc ccgatgaaca caccgtcatc cgctgtatcc
    47461 tgccaccgga agatgaacgc gacagacacc ccggtgaatc cctgctgaaa accacgtacc
    47521 gctacaacgc cgccggagaa ctgacagagg tcattctgcc gggggatgag acgctgacgt
    47581 tcagccgtga tgaggcggga cgtgaagtgc tccggcacag taaccggggt tttgcctgtg
    47641 aacagggctg gaatgcagcc ggtcagcctg tcagtcagcg cgccggattt tttccggcag
    47701 aagccacatg ggacgggctg gttccctcac tggtacggga gtaccgttac gacagcgcgg
    47761 gtaacgtgtc aggcgtcacc agccgggaag attacggacg ggaaacacgg cgggagtatc
    47821 ggctggaccg gaacggccag gtcacggcgg tgacagcctc aggcaccggg ctgggctatg
    47881 gcgaaggcga tgagtcctat ggctatgaca gctgcggcta cctgaaggcg cagtctgcgg
    47941 gcaggcaccg gataagtgaa gagactgacc agtatgccgg aggccaccgg ctgaaacagg
    48001 ccggaaacac gcagtatgac tatgacgccg caggccggat ggtcagccgc accagacacc
    48061 gtgacggcta ccgaccggaa acagagcggt tccggtggga cagccgggac cagctgaccg
    48121 ggtattgcag cgcacagggt gagctgtggg aataccgcca cgacgccagc ggcagacgaa
    48181 cagagaaacg ctgcgaccgg aagaaaatcc gcttcacgta tctgtgggac ggcgacagta
    48241 ttgcggaaat ccgggaatac cgcgatgata aactgtacag cgtaaggcac ctggtgttta
    48301 acggctttga gctgataagc cagcagttca gccgggtacg gcaggcgcat ccgtcggtgg
    48361 ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc cgcccgctga
    48421 tgctctttaa cagtgaagga aagacggtct ggcgaccggg gcagaccagc ctgtgggggc
    48481 tggcactcag cctgcccgca gacaccggct acccggaccc gcgcggggaa ctggacccgg
    48541 aagccgaccc cggcctgctg tatgcaggac agtggcagga tgcggaatcc gggctgtgct
    48601 ataatcggtt ccggtattac gagccggaaa ccggaatgta cctggtgagt gatccgctgg
    48661 ggttgcaggg cggggagcag acttaccggt atgtgccgaa tccttgtggg tgggttgatc
    48721 cgctgggatt ggctgcaagt tctaaaatca gcagtttgat ggactatatt ggcgatggtc
    48781 gtcgtgttag tgggcatacg ggtttcctgg atggggttcg tttatcacgt agtcaaataa
//
LOCUS       DAEEBB010000012        46684 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEEBB010000012 DAEEBB010000000
VERSION     DAEEBB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.012"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="KLF07_000684"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="KLF07_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBD9830795.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="KLF07_000685"
     CDS             complement(280..873)
                     /locus_tag="KLF07_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD9830796.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="KLF07_000686"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="KLF07_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD9830797.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="KLF07_000687"
     CDS             1788..2183
                     /locus_tag="KLF07_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD9830798.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="KLF07_000688"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="KLF07_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBD9830799.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="KLF07_000689"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="KLF07_000689"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBD9830800.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="KLF07_000690"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="KLF07_000690"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBD9830801.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="KLF07_000691"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="KLF07_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBD9830802.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="KLF07_000692"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="KLF07_000692"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBD9830803.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="KLF07_000693"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="KLF07_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBD9830804.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="KLF07_000694"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="KLF07_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBD9830805.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="KLF07_000695"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="KLF07_000695"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBD9830806.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="KLF07_000696"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="KLF07_000696"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBD9830807.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="KLF07_000697"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="KLF07_000697"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBD9830808.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="KLF07_000698"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="KLF07_000698"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBD9830809.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="KLF07_000699"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="KLF07_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBD9830810.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="KLF07_000700"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="KLF07_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBD9830811.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="KLF07_000701"
     CDS             17158..17709
                     /locus_tag="KLF07_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBD9830812.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="KLF07_000702"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="KLF07_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBD9830813.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="KLF07_000703"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="KLF07_000703"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBD9830814.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="KLF07_000704"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="KLF07_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBD9830815.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="KLF07_000705"
     CDS             complement(21354..22376)
                     /locus_tag="KLF07_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9830816.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="KLF07_000706"
     CDS             complement(22390..23892)
                     /locus_tag="KLF07_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD9830817.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="KLF07_000707"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="KLF07_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBD9830818.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="KLF07_000708"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="KLF07_000708"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBD9830819.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="KLF07_000709"
     CDS             complement(26207..26548)
                     /locus_tag="KLF07_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBD9830820.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="KLF07_000710"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="KLF07_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBD9830821.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="KLF07_000711"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="KLF07_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBD9830822.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="KLF07_000712"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="KLF07_000712"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBD9830823.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="KLF07_000713"
     CDS             33227..34570
                     /locus_tag="KLF07_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD9830824.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="KLF07_000714"
     CDS             complement(34650..34856)
                     /locus_tag="KLF07_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBD9830825.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="KLF07_000715"
     CDS             35181..35735
                     /locus_tag="KLF07_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD9830826.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="KLF07_000716"
     CDS             complement(35825..36736)
                     /locus_tag="KLF07_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830827.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="KLF07_000717"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="KLF07_000717"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBD9830828.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="KLF07_000718"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="KLF07_000718"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBD9830829.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDAPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="KLF07_000719"
     CDS             complement(39939..40319)
                     /locus_tag="KLF07_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD9830830.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="KLF07_000720"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="KLF07_000720"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD9830831.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="KLF07_000721"
     CDS             41376..42341
                     /locus_tag="KLF07_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD9830832.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="KLF07_000722"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="KLF07_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBD9830833.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="KLF07_000723"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="KLF07_000723"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBD9830834.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="KLF07_000724"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="KLF07_000724"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD9830835.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="KLF07_000725"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="KLF07_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBD9830836.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46684)
                     /locus_tag="KLF07_000726"
     CDS             complement(46338..>46684)
                     /locus_tag="KLF07_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD9830837.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11453 a        12416 c        11632 g        11183 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgctccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggt
//
LOCUS       DAEEBB010000013        45922 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEEBB010000013 DAEEBB010000000
VERSION     DAEEBB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.013"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..715)
                     /locus_tag="KLF07_000727"
     CDS             complement(<1..715)
                     /locus_tag="KLF07_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD9830838.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="KLF07_000728"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="KLF07_000728"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBD9830839.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="KLF07_000729"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="KLF07_000729"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBD9830840.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="KLF07_000730"
     CDS             complement(3649..4704)
                     /locus_tag="KLF07_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD9830841.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="KLF07_000731"
     CDS             complement(4714..5502)
                     /locus_tag="KLF07_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBD9830842.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="KLF07_000732"
     CDS             complement(5520..6572)
                     /locus_tag="KLF07_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBD9830843.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7206)
                     /locus_tag="KLF07_000733"
                     /pseudo
     CDS             complement(6603..7206)
                     /locus_tag="KLF07_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7193..8074)
                     /locus_tag="KLF07_000734"
     CDS             complement(7193..8074)
                     /locus_tag="KLF07_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBD9830844.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8095..9405)
                     /locus_tag="KLF07_000735"
     CDS             complement(8095..9405)
                     /locus_tag="KLF07_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9830845.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9440..10950)
                     /locus_tag="KLF07_000736"
                     /pseudo
     CDS             complement(9440..10950)
                     /locus_tag="KLF07_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="KLF07_000737"
     CDS             complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="KLF07_000737"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBD9830846.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="KLF07_000738"
     CDS             complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="KLF07_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBD9830847.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="KLF07_000739"
     CDS             complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="KLF07_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBD9830848.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="KLF07_000740"
     CDS             complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="KLF07_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBD9830849.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="KLF07_000741"
     CDS             complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="KLF07_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBD9830850.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13253..14230
                     /gene="pspF"
                     /locus_tag="KLF07_000742"
     CDS             13253..14230
                     /gene="pspF"
                     /locus_tag="KLF07_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBD9830851.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="KLF07_000743"
                     /pseudo
     CDS             complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="KLF07_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="KLF07_000744"
     CDS             complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="KLF07_000744"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBD9830852.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="KLF07_000745"
                     /pseudo
     CDS             complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="KLF07_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="KLF07_000746"
     CDS             complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="KLF07_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBD9830853.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="KLF07_000747"
     CDS             complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="KLF07_000747"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBD9830854.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20133..21494
                     /gene="puuA"
                     /locus_tag="KLF07_000748"
     CDS             20133..21494
                     /gene="puuA"
                     /locus_tag="KLF07_000748"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBD9830855.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21643..21807
                     /locus_tag="KLF07_000749"
     CDS             21643..21807
                     /locus_tag="KLF07_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830856.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21797..23182
                     /gene="puuP"
                     /locus_tag="KLF07_000750"
     CDS             21797..23182
                     /gene="puuP"
                     /locus_tag="KLF07_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBD9830857.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23316..23561
                     /locus_tag="KLF07_000751"
     CDS             23316..23561
                     /locus_tag="KLF07_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBD9830858.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23874..25517
                     /gene="sapA"
                     /locus_tag="KLF07_000752"
     CDS             23874..25517
                     /gene="sapA"
                     /locus_tag="KLF07_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBD9830859.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25514..26479
                     /gene="sapB"
                     /locus_tag="KLF07_000753"
     CDS             25514..26479
                     /gene="sapB"
                     /locus_tag="KLF07_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBD9830860.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26466..27356
                     /gene="sapC"
                     /locus_tag="KLF07_000754"
     CDS             26466..27356
                     /gene="sapC"
                     /locus_tag="KLF07_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBD9830861.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27356..28348
                     /gene="sapD"
                     /locus_tag="KLF07_000755"
     CDS             27356..28348
                     /gene="sapD"
                     /locus_tag="KLF07_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBD9830862.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28350..29156
                     /gene="sapF"
                     /locus_tag="KLF07_000756"
     CDS             28350..29156
                     /gene="sapF"
                     /locus_tag="KLF07_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBD9830863.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29224..29577
                     /locus_tag="KLF07_000757"
     CDS             29224..29577
                     /locus_tag="KLF07_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBD9830864.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29945..30733
                     /gene="fabI"
                     /locus_tag="KLF07_000758"
     CDS             29945..30733
                     /gene="fabI"
                     /locus_tag="KLF07_000758"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBD9830865.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30878..32004
                     /locus_tag="KLF07_000759"
                     /pseudo
     CDS             30878..32004
                     /locus_tag="KLF07_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32072..34006
                     /gene="rnb"
                     /locus_tag="KLF07_000760"
     CDS             32072..34006
                     /gene="rnb"
                     /locus_tag="KLF07_000760"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBD9830866.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34084..34187)
                     /locus_tag="KLF07_000761"
                     /pseudo
     CDS             complement(34084..34187)
                     /locus_tag="KLF07_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34241..36231
                     /gene="pdeR"
                     /locus_tag="KLF07_000762"
                     /pseudo
     CDS             34241..36231
                     /gene="pdeR"
                     /locus_tag="KLF07_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36378..36551
                     /locus_tag="KLF07_000763"
     CDS             36378..36551
                     /locus_tag="KLF07_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830867.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36641..37390
                     /locus_tag="KLF07_000764"
     CDS             36641..37390
                     /locus_tag="KLF07_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD9830868.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37659..37877
                     /gene="osmB"
                     /locus_tag="KLF07_000765"
     CDS             37659..37877
                     /gene="osmB"
                     /locus_tag="KLF07_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBD9830869.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="KLF07_000766"
     CDS             complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="KLF07_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBD9830870.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="KLF07_000767"
     CDS             complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="KLF07_000767"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBD9830871.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="KLF07_000768"
     CDS             complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="KLF07_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBD9830872.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="KLF07_000769"
     CDS             complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="KLF07_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBD9830873.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="KLF07_000770"
     CDS             complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="KLF07_000770"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBD9830874.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41826..42416
                     /gene="ribA"
                     /locus_tag="KLF07_000771"
     CDS             41826..42416
                     /gene="ribA"
                     /locus_tag="KLF07_000771"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBD9830875.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="KLF07_000772"
     CDS             complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="KLF07_000772"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBD9830876.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45319..45414)
                     /locus_tag="KLF07_000773"
     CDS             complement(45319..45414)
                     /locus_tag="KLF07_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830877.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45528..45695)
                     /locus_tag="KLF07_000774"
     CDS             complement(45528..45695)
                     /locus_tag="KLF07_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830878.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45698..45862)
                     /locus_tag="KLF07_000775"
     CDS             complement(45698..45862)
                     /locus_tag="KLF07_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBD9830879.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10847 a        12140 c        11560 g        11375 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7081 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7141 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7201 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7261 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7321 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7381 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7441 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7501 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7561 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7621 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7681 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7741 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7801 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7861 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7921 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7981 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8041 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8101 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8161 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8221 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8281 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8341 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8401 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8461 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8521 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8581 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8641 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8701 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8761 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8821 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     8881 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8941 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9001 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9061 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9121 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9181 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9241 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9301 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9361 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9421 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     9481 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9541 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     9601 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9661 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9721 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9781 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9841 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9901 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9961 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10021 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10081 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10141 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10201 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10261 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10321 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10381 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10441 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10501 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10561 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10621 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10681 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10741 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10801 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10861 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10921 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10981 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11041 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11101 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11161 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11221 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11281 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11341 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11401 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11461 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11521 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11581 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11641 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11701 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11761 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11821 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11881 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccagc
    11941 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12001 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12061 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12121 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12181 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12241 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12301 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12361 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12421 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12481 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12541 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12601 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12661 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12721 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12781 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12841 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12901 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12961 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13021 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13081 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13141 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13201 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13261 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13321 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13381 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13441 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13501 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13561 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13621 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13681 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13741 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13801 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13861 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13921 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13981 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14041 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14101 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14161 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14221 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14281 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14341 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14401 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14461 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14521 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14581 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14641 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14701 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14761 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14821 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14881 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14941 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15001 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15061 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15121 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15181 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15241 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15301 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15361 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15421 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15481 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15541 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15601 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15661 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15721 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15781 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15841 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15901 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15961 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16021 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16081 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16141 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16201 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16261 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16321 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16381 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16441 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16501 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16561 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16621 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16681 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16741 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16801 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16861 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16921 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16981 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17041 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17101 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17161 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17221 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17281 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17341 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17401 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17461 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17521 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17581 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17641 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17701 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17761 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17821 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17881 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17941 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18001 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18061 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18121 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18181 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18241 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    18301 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18361 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18421 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18481 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18541 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18601 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18661 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18721 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18781 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18841 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18901 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18961 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19021 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19081 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19141 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19201 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19261 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19321 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19381 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19441 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19501 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19561 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19621 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19681 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19741 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19801 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19861 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19921 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19981 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20041 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20101 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20161 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20221 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20281 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20341 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20401 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20461 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20521 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20581 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20641 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20701 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20761 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20821 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20881 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20941 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21001 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21061 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21121 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21181 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21241 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21301 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21361 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21421 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21481 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21541 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21601 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21661 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21721 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21781 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21841 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21901 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21961 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22021 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22081 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22141 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22201 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22261 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22321 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22381 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22441 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22501 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22561 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22621 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22681 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22741 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22801 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22861 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22921 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22981 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23041 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23101 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23161 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23221 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    23281 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23341 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23401 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23461 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23521 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23581 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23641 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23701 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23761 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23821 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23881 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23941 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24001 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24061 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24121 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24181 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24241 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24301 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24361 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24421 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24481 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24541 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24601 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24661 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24721 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24781 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24841 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24901 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24961 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25021 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25081 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25141 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    25201 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25261 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25321 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25381 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25441 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25501 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25561 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25621 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25681 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25741 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25801 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25861 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25921 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25981 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26041 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26101 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26161 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26221 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26281 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26341 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26401 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26461 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26521 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26581 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26641 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26701 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26761 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26821 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26881 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26941 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27001 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27061 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27121 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27181 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27241 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27301 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27361 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27421 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27481 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27541 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27601 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27661 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27721 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27781 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27841 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27901 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27961 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28021 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28081 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28141 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28201 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28261 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28321 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28381 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28441 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28501 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28561 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28621 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28681 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28741 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28801 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28861 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28921 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28981 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29041 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29101 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29161 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29221 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29281 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29341 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29401 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29461 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29521 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29581 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29641 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29701 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29761 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29821 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29881 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29941 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30001 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30061 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30121 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30181 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30241 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30301 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30361 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30421 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30481 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30541 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30601 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30661 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30721 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30781 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30841 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30901 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30961 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31021 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31081 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31141 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31201 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31261 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31321 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31381 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31441 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31501 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31561 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31621 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31681 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31741 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31801 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31861 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31921 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31981 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32041 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32101 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32161 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    32221 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32281 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32341 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32401 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32461 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32521 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32581 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32641 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32701 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32761 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32821 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32881 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32941 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33001 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33061 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33121 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33181 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33241 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33301 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33361 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33421 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33481 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33541 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33601 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33661 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33721 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33781 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33841 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33901 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33961 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34021 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34081 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34141 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34201 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34261 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34321 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34381 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34441 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34501 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34561 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34621 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34681 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34741 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34801 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34861 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34921 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34981 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    35041 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35101 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35161 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35221 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35281 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35341 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35401 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35461 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35521 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35581 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35641 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35701 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35761 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35821 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35881 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35941 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36001 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36061 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36121 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36181 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36241 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36301 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36361 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36421 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36481 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36541 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36601 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36661 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36721 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36781 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36841 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    36901 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36961 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37021 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37081 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37141 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37201 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37261 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37321 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37381 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37441 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37501 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37561 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37621 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37681 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37741 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37801 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37861 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37921 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37981 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38041 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38101 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38161 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38221 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38281 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38341 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38401 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38461 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38521 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38581 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38641 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38701 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38761 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38821 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38881 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38941 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39001 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39061 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39121 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39181 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39241 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39301 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39361 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39421 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39481 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39541 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39601 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39661 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39721 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39781 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39841 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39901 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39961 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40021 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40081 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40141 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40201 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40261 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40321 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40381 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40441 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40501 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40561 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40621 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40681 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40741 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40801 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40861 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40921 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40981 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41041 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41101 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41161 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41221 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41281 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41341 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41401 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    41461 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41521 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41581 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41641 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41701 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41761 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41821 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41881 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41941 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42001 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42061 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42121 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42181 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42241 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42301 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42361 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42421 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42481 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42541 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42601 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42661 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42721 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42781 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42841 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42901 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42961 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43021 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43081 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43141 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43201 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43261 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43321 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43381 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43441 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43501 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43561 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43621 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43681 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43741 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43801 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43861 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43921 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43981 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44041 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44101 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44161 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44221 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44281 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44341 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44401 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44461 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44521 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44581 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44641 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44701 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44761 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44821 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44881 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44941 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45001 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45061 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45121 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45181 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45241 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45301 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45361 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45421 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45481 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45541 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45601 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45661 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45721 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45781 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45841 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45901 caaatcctaa agaaacttta gt
//
LOCUS       DAEEBB010000014        43713 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEEBB010000014 DAEEBB010000000
VERSION     DAEEBB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.014"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..559)
                     /locus_tag="KLF07_000776"
     CDS             complement(<1..559)
                     /locus_tag="KLF07_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBD9830880.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="KLF07_000777"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="KLF07_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBD9830881.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="KLF07_000778"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="KLF07_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBD9830882.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="KLF07_000779"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="KLF07_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBD9830883.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="KLF07_000780"
     CDS             complement(4001..6322)
                     /locus_tag="KLF07_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBD9830884.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="KLF07_000781"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="KLF07_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBD9830885.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="KLF07_000782"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="KLF07_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBD9830886.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="KLF07_000783"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="KLF07_000783"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBD9830887.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="KLF07_000784"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="KLF07_000784"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBD9830888.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="KLF07_000785"
     CDS             11268..11582
                     /locus_tag="KLF07_000785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD9830889.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="KLF07_000786"
     CDS             11595..12968
                     /locus_tag="KLF07_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBD9830890.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="KLF07_000787"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="KLF07_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBD9830891.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="KLF07_000788"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="KLF07_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBD9830892.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="KLF07_000789"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="KLF07_000789"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBD9830893.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="KLF07_000790"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="KLF07_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBD9830894.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="KLF07_000791"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="KLF07_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBD9830895.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="KLF07_000792"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="KLF07_000792"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBD9830896.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="KLF07_000793"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="KLF07_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBD9830897.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="KLF07_000794"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="KLF07_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="KLF07_000795"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="KLF07_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBD9830898.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="KLF07_000796"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="KLF07_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBD9830899.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="KLF07_000797"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="KLF07_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBD9830900.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="KLF07_000798"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="KLF07_000798"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBD9830901.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="KLF07_000799"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="KLF07_000799"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBD9830902.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="KLF07_000800"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="KLF07_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBD9830903.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="KLF07_000801"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="KLF07_000801"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD9830904.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="KLF07_000802"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="KLF07_000802"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBD9830905.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="KLF07_000803"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="KLF07_000803"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBD9830906.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="KLF07_000804"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="KLF07_000804"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBD9830907.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="KLF07_000805"
     CDS             31280..32185
                     /locus_tag="KLF07_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830908.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="KLF07_000806"
     CDS             32202..32564
                     /locus_tag="KLF07_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBD9830909.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="KLF07_000807"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="KLF07_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="KLF07_000808"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="KLF07_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="KLF07_000809"
     CDS             35034..35912
                     /locus_tag="KLF07_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBD9830910.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="KLF07_000810"
     CDS             35923..36276
                     /locus_tag="KLF07_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBD9830911.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="KLF07_000811"
     CDS             36288..37592
                     /locus_tag="KLF07_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBD9830912.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="KLF07_000812"
     CDS             37604..38689
                     /locus_tag="KLF07_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD9830913.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="KLF07_000813"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="KLF07_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBD9830914.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="KLF07_000814"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="KLF07_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBD9830915.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="KLF07_000815"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="KLF07_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="KLF07_000816"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="KLF07_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBD9830916.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="KLF07_000817"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="KLF07_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBD9830917.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11126 c        11988 g        10442 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cattcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaactcg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DAEEBB010000015        41013 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEEBB010000015 DAEEBB010000000
VERSION     DAEEBB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.015"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="KLF07_000818"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="KLF07_000818"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBD9830918.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="KLF07_000819"
     tRNA            2260..2336
                     /locus_tag="KLF07_000819"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="KLF07_000820"
     tRNA            2345..2429
                     /locus_tag="KLF07_000820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="KLF07_000821"
     tRNA            2453..2527
                     /locus_tag="KLF07_000821"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="KLF07_000822"
     tRNA            2562..2636
                     /locus_tag="KLF07_000822"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="KLF07_000823"
     tRNA            2652..2728
                     /locus_tag="KLF07_000823"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="KLF07_000824"
     tRNA            2777..2851
                     /locus_tag="KLF07_000824"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="KLF07_000825"
     tRNA            2888..2962
                     /locus_tag="KLF07_000825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="KLF07_000826"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="KLF07_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBD9830919.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="KLF07_000827"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="KLF07_000827"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBD9830920.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="KLF07_000828"
     CDS             6014..7054
                     /locus_tag="KLF07_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBD9830921.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="KLF07_000829"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="KLF07_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBD9830922.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="KLF07_000830"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="KLF07_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBD9830923.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="KLF07_000831"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="KLF07_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBD9830924.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="KLF07_000832"
     CDS             complement(10127..12004)
                     /locus_tag="KLF07_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBD9830925.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="KLF07_000833"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="KLF07_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBD9830926.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="KLF07_000834"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="KLF07_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBD9830927.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="KLF07_000835"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="KLF07_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBD9830928.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="KLF07_000836"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="KLF07_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBD9830929.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="KLF07_000837"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="KLF07_000837"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBD9830930.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="KLF07_000838"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="KLF07_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBD9830931.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20005)
                     /gene="djlC"
                     /locus_tag="KLF07_000839"
     CDS             complement(18551..20005)
                     /gene="djlC"
                     /locus_tag="KLF07_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBD9830932.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(20002..20709)
                     /locus_tag="KLF07_000840"
     CDS             complement(20002..20709)
                     /locus_tag="KLF07_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD9830933.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20811..21365
                     /locus_tag="KLF07_000841"
     CDS             20811..21365
                     /locus_tag="KLF07_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD9830934.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21375..22802)
                     /locus_tag="KLF07_000842"
     CDS             complement(21375..22802)
                     /locus_tag="KLF07_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBD9830935.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22799..23506)
                     /locus_tag="KLF07_000843"
     CDS             complement(22799..23506)
                     /locus_tag="KLF07_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD9830936.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23670..24646
                     /locus_tag="KLF07_000844"
                     /pseudo
     CDS             23670..24646
                     /locus_tag="KLF07_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            complement(24716..25198)
                     /locus_tag="KLF07_000845"
     CDS             complement(24716..25198)
                     /locus_tag="KLF07_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBD9830937.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25433..28015
                     /gene="leuS"
                     /locus_tag="KLF07_000846"
     CDS             25433..28015
                     /gene="leuS"
                     /locus_tag="KLF07_000846"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBD9830938.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28030..28611
                     /gene="lptE"
                     /locus_tag="KLF07_000847"
     CDS             28030..28611
                     /gene="lptE"
                     /locus_tag="KLF07_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBD9830939.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28611..29642
                     /gene="holA"
                     /locus_tag="KLF07_000848"
     CDS             28611..29642
                     /gene="holA"
                     /locus_tag="KLF07_000848"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBD9830940.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29644..30285
                     /gene="nadD"
                     /locus_tag="KLF07_000849"
     CDS             29644..30285
                     /gene="nadD"
                     /locus_tag="KLF07_000849"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBD9830941.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30309..30920
                     /locus_tag="KLF07_000850"
                     /pseudo
     CDS             30309..30920
                     /locus_tag="KLF07_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31180..31497
                     /gene="rsfS"
                     /locus_tag="KLF07_000851"
                     /gene_synonym="rsfA"
     CDS             31180..31497
                     /gene="rsfS"
                     /locus_tag="KLF07_000851"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBD9830942.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31501..31968
                     /gene="rlmH"
                     /locus_tag="KLF07_000852"
     CDS             31501..31968
                     /gene="rlmH"
                     /locus_tag="KLF07_000852"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBD9830943.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31999..33900
                     /gene="mrdA"
                     /locus_tag="KLF07_000853"
     CDS             31999..33900
                     /gene="mrdA"
                     /locus_tag="KLF07_000853"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBD9830944.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33903..35015
                     /gene="mrdB"
                     /locus_tag="KLF07_000854"
                     /gene_synonym="rodA"
     CDS             33903..35015
                     /gene="mrdB"
                     /locus_tag="KLF07_000854"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBD9830945.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35026..36114
                     /gene="rlpA"
                     /locus_tag="KLF07_000855"
     CDS             35026..36114
                     /gene="rlpA"
                     /locus_tag="KLF07_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBD9830946.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36253..37464
                     /gene="dacA"
                     /locus_tag="KLF07_000856"
     CDS             36253..37464
                     /gene="dacA"
                     /locus_tag="KLF07_000856"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBD9830947.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37574..37837
                     /locus_tag="KLF07_000857"
     CDS             37574..37837
                     /locus_tag="KLF07_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBD9830948.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37938..38579
                     /gene="lipB"
                     /locus_tag="KLF07_000858"
     CDS             37938..38579
                     /gene="lipB"
                     /locus_tag="KLF07_000858"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBD9830949.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38839..39792
                     /locus_tag="KLF07_000859"
     CDS             38839..39792
                     /locus_tag="KLF07_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD9830950.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            40001..40966
                     /gene="lipA"
                     /locus_tag="KLF07_000860"
     CDS             40001..40966
                     /gene="lipA"
                     /locus_tag="KLF07_000860"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBD9830951.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10299 a        10449 c        10291 g         9974 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagaccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc agtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacga cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctgg cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagtatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcat
    19561 ccattccttc gctgtcatta aacgaatgcc agttcaaacg ctctgccaac aaagaagcac
    19621 aatttaatga aagatatcgc gcttctatag cgattgcaca cagcggccag cgtaattgat
    19681 cgacatcctc catgttccag gtatttaatt gctgaataaa tttctgccag gcggagggct
    19741 gaaaacgatc actttctgaa tccagtaatg tcctgaatgc acgtagtatt tcatgttcag
    19801 cggtggcatc tttttcttca tcatcggctt cctcgacagg atttaccgcc agtcgcagcg
    19861 cctcttcata ggcctggcgt aatgctttaa acccctgcgg atcggtttcg ggatgacaca
    19921 acggtaagcg agcaagataa gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt
    19981 cgaggatttg ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta ccgggcattc
    20041 aggctggaca gttaatggtt gccagggaag cgtggcatag aaacggtcgc ttcccgctgc
    20101 cagctcttcg aacatccgac tcccggcgct atcgtatttg tacagggtta gcgcctcgcg
    20161 tagcgaagcg ttgtcttcgc actgagaaga ttgccagtag agacgcccta gtgaataggc
    20221 agaaaaatag tgcatccagc tgtgatagta atggtgagct cgtgcataga cgcgcgattg
    20281 cagccataaa ctctcttctt ccgtcagcca gttgttgagc acaccgaccc gtgacagaaa
    20341 ccccattcga acataatccc aggctttgat tcccccttct ccacagcaca tagcggtttg
    20401 ggcaacgaat tgggtataaa tcaaccctct ctccgagcca ccagcaatta atgctttcct
    20461 ctcttcaggg gaagagcgtt gccatgtcag atataagcca gctaaatcat tggcatgacc
    20521 atcatcagtc atctgataaa tagtctgaat taaatcatca cgattattta ttccccagga
    20581 tgatgagaga tcgaattctt ttatcccagt atacattttt ggcgagtcat gcgcgggttt
    20641 gcaactctga ttaagtattt ccatgggcgc agaaagcgca aaaagaagat attgttcttc
    20701 cttattcatt ttttaataat cccttaagtg cattaactga ctactttttt attattgatg
    20761 agtataaact tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac atgaataaaa
    20821 aatcaatgta tatattcgca atttttatag ttgcagcaat tacctgtatt agccaaccca
    20881 agaaaacgac gttgcgtgat aaagccatgg tgaattatgc ctttgattat ttaagatcac
    20941 caggcagtct accgttaacc acggcagcca cggagctttc cgcgattcat ggtcactcaa
    21001 catcgcaata tcgccttggc gaattttatc ttcatggtag cgacggtaaa ccactggatt
    21061 atacacaggc gagatactgg tatgagcaat cagcagaaca ggaaaatccg cgcgcacaaa
    21121 gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa acccgatacc cgtaaagcaa
    21181 ttctctggta taaggaagca gctgaacaag ggtatgctca tgctcaatat actttgggtt
    21241 tgatctacag aaatggcact ggtattaatg ttaaccatta tgaatctcaa aaatggttaa
    21301 aactggccgc caaacaacat tacaaaaatg cggaaagatt acttgccgga cttcccgcac
    21361 attaacgtcg actgtcaccc gaagagatgg agtatttttc ctatcaaacc ggcaaagata
    21421 aaaatgtaga aaataatttt gatgacgccc agacagccca gcccgccatc gtcaacgggc
    21481 attggctgcg gttgcggctc cggctcgacg gtttcctgac cggtaaaatt cgccgcctga
    21541 attaataatc gacgaataat taatgcttcc agtggacaac gatcttctgc atcaaggcgg
    21601 ctcacaatcg gcattaatga ttcttctgtc ggttggctgg cctgcactaa ttgtttggca
    21661 actaaatata atggccataa gggatgttct gtatcacgcg tgagtgagat ccgcggctta
    21721 cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag tttttcatcg
    21781 ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt cgccggggac ataaagtagc
    21841 tgtttaccct gatcgttaac cagttcacga gcttcaatca tcatcaccac taacgggtaa
    21901 taatcgaccc gatgctgacg gcaccattgc aatattaaag catccgcctg ggtggaggga
    21961 tactcgcgcc agtagtcaca aagttctgcc agcaagcttt cgccttctcc acaatagacg
    22021 cgttgcgcgt attcataata acgcgcatct tcattatcag gttcagcttc ccgccacgac
    22081 cgggcgtaat ccagaagttc ggggatatcc aggcgacctg cgctatacca gcggagtaaa
    22141 tttctgtgca gttttgcatc atcggggatg atcactgcgc gatgttgcgc taaatacaat
    22201 gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg
    22261 tcattttgca cggcaacggg cagatgcaga atggtctgga agttaaaaac attccccttc
    22321 gcaatggcat ataaaaagga ttccagttct tcttcgtcta tttccccgac gttttcctcc
    22381 tgcaaccacc gcaatctttc tgctaataac accacgcact cgaaggataa atgggcagtg
    22441 ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa tctcttccat cgagcaataa
    22501 tttaattgct gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct atcagaggca
    22561 agtaacgcgc gaaaggcgag cagaatttca tgttctgcta cctcatattc ttctggttgc
    22621 caaacagatt tagcaggcga ctgcgcaatc cggagcgcct cctcatacgc ctgacgaagt
    22681 tgtttaaaac cctgcggatc ggtttctgga tgaaaggaca gtaacagcgc cagataagcg
    22741 cggcggataa tatcgacgtc agtcgtttcc tctatttcga ggatcttcca gcaatttttc
    22801 ataggtcgct catatcctta agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc
    22861 aggacaaggc agggtaatac gcatcatcac tggcaaataa ttgataaaac atatcattgc
    22921 cggaatcgtc gtattcttta cgagctaaga attcgcgtaa taaaggcaga tgatcatctt
    22981 cgggagactg ccaatattgc cgaccaaagg tataaccggc aaagtattgc cgccagttac
    23041 tataataacg tagagcccgt agatgaatac gcgactggat ccatagactt tcttcttcac
    23101 tcaaccagtt attaagtacg cccattcgac ttaaaaaacc catccggaca taatcccatg
    23161 ctttaattcc tccctcacca cagcattcgg ctgtgctggc gacaaattgc gcataagcct
    23221 gaccttgctc atttagttca gcaagatagt cacgccattc acatggcgaa taacgaaacc
    23281 agcggtgata aaaccctgcc aaccgagcgg catgaccgtt atcgatcatt cggtgaattg
    23341 tgcgcagcag gtcatcacgg tcatcaattc cccatgagga agaaaggtca aaaaagcatg
    23401 tacctaaata cattttgggg gatgaatgcg aaggtaaaca gcattcattg cgtatttcca
    23461 tcggagtgga aagtgcaaac aatatttttt gcgaccccat gtccatataa tttatcctta
    23521 aacattcatt taatgaatgt aaactacatt tcgagactca gttatttata cttttctgac
    23581 gcaacataat acgcataaaa taaggcttac gcaaaaatgg aacacataaa aatataatat
    23641 gttttatcta caaccataag gaaatgataa tggtttttac gtcaagttgc tgcgataatt
    23701 tatcaataga tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct caatatattg
    23761 ttgggtttta ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa aaggcctttt
    23821 actggctgaa gctggccgct gagcaaggtc attgtgaagc acagtattcc ttagggcaaa
    23881 agtataccga ggataaaagc cgtcataaag ataatgagca agccatcttc tggctgaaaa
    23941 aagctgccct acaaggccat actttcgctt ccaacgccct tggctggaca ctggatcgtg
    24001 gagaagcccc caattataaa gaagcggttg tctggtatca gatagccgcg gagagcggaa
    24061 tgtcttatgc gcaaaataat cttgggtgga tgtacagaaa tggcaacgga gtcgcaaaag
    24121 actatgcgct ggcatttttc tggtacaaac aagctgcatt acaaggccat agtgacgcgc
    24181 aaaacaatct ggccgatctt tatgaagacg gaaaaggcgt tgctcaaaac aagacactcg
    24241 ccgcattctg gtatttgaaa agcgcacagc agggtaatcg gcacgcccag tttcaaattg
    24301 catgggatta taacgctggc gaaggggtgg accaggacta taagcaagcg atgtactggt
    24361 atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt tatatgtata
    24421 aacacggaca aggcgttgag aaagattatc aggctgcctt tgaatggttt acgaaagccg
    24481 ctgaatgcaa tgacgccact gcctggtata acctggccat tattatcact acggagaagg
    24541 aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac
    24601 cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagagat aaaaacaagg
    24661 gccggggaat ttcattcggc ccatattctc tttattgctg tgcgaaatca gactattacg
    24721 gctcaaacgg gcgtctctgg aactggtcat gaccacattt cgggcatagc gtcagcactt
    24781 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga
    24841 ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact tcgcgccatt
    24901 caagctgcgt tttatcggtg atatctgcca gctcctgcca taagctttct ttaatcaccc
    24961 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
    25021 ccaggtcacg tcttacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
    25081 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
    25141 gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
    25201 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
    25261 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
    25321 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
    25381 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
    25441 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
    25501 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
    25561 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
    25621 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg
    25681 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga
    25741 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
    25801 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
    25861 gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc cgaacgacca
    25921 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
    25981 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
    26041 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
    26101 gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg acaacatgct
    26161 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagctgc
    26221 gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg cctttattga
    26281 cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
    26341 cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
    26401 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
    26461 gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccagtta tcctggctgc
    26521 tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg tgctgttcaa
    26581 ctctggcgaa ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
    26641 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
    26701 tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
    26761 gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
    26821 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
    26881 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
    26941 cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact actggctgcc
    27001 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
    27061 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
    27121 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
    27181 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
    27241 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
    27301 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
    27361 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggtgt
    27421 ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa
    27481 aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg
    27541 cgatgtgcat aaaacgatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
    27601 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac caaccgatgg
    27661 cgagcaggat cgcgctctga tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc
    27721 gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga
    27781 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
    27841 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ctggtggacg caacggaaga
    27901 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
    27961 cgtgcgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
    28021 ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc
    28081 gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa ggtcatgatc
    28141 ctggactcag gcgatccgaa cgggccatta agccgtgcag tgcgtaacca gttacgtctg
    28201 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cctgcgtttg
    28261 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
    28321 tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga tatctacccg
    28381 attagcgcca aagtcttccg ttcgttcttt gataacccgc aaatggcgtt agcgaaagat
    28441 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
    28501 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
    28561 acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg atgattcggt
    28621 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
    28681 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg
    28741 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
    28801 tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
    28861 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
    28921 tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg
    28981 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
    29041 aggctcagct tccccgctgg gttactgcgc gcgcaaaaca gctcaactta gaactggatg
    29101 acgcggcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
    29161 cactggagcg tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac
    29221 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
    29281 gaaaaagtaa gcgcgcattg catattcttc agcaactgcg tctggaaggc agcgaaccgg
    29341 ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg aaacgccagt
    29401 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
    29461 gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac
    29521 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
    29581 tgaaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
    29641 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
    29701 ctaaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aatcatccct
    29761 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
    29821 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
    29881 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca aggaccggac
    29941 gtgccgctgg cgtttattat tggtcaggat tcactgctga cctttccgac ctggtacgaa
    30001 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
    30061 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
    30121 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg gtttaacatc
    30181 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
    30241 gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
    30301 agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc
    30361 tttacagcgg tcatgcgccc acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc
    30421 tgcatacgct gctacatggt gtttcctttg atctggtttt atgcagtgaa ctggaacggg
    30481 cacagcatac cgcacgactg gttctcagtg accgccagct ccccgtgcaa atcatccctg
    30541 aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag
    30601 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgcaatc cccacgaacg
    30661 gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat
    30721 ttcagcacta tcagaatatt ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg
    30781 cccgtttaat tggcatgcct gccgaggcta tgtggcattt tcgcgttgac taggggtgtt
    30841 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
    30901 tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
    30961 cagacccggg caggctgata ttctccgcag ccagactttt ttcgccagac acgactttgt
    31021 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
    31081 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
    31141 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
    31201 gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
    31261 caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
    31321 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
    31381 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
    31441 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
    31501 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
    31561 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
    31621 ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
    31681 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
    31741 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta
    31801 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg
    31861 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
    31921 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt
    31981 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
    32041 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
    32101 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca gactcgctct
    32161 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
    32221 aatggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat gccagagaaa
    32281 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac
    32341 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattccggtg
    32401 aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
    32461 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
    32521 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
    32581 aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat
    32641 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
    32701 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
    32761 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt
    32821 gtcaccgatc cgcgtacagg tggggtgctg gcgctggttt ccacgcctag ttatgaccca
    32881 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgttgaa cgccccgaat
    32941 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc
    33001 tatgtggcgg tttcggcatt gagcgccggg gtgatcacgc gcaatacgac gctgtttgac
    33061 ccaggctggt ggcaactgcc aggttcggaa aaacgttatc gtgactggaa aaaatggggc
    33121 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
    33181 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
    33241 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
    33301 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggt
    33361 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
    33421 gacggtatcg tgaaggttcc tcatttgctg atgagcaccg ccgaagacgg caaacaggtg
    33481 ccatgggtac agccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
    33541 gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca taaatacttt
    33601 gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt cggtctgaaa
    33661 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    33721 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggt
    33781 ggtgcgggtc cggcggttgg tacactgatg cgccagatcc tcgaccacat tatgctgggt
    33841 gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa
    33901 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    33961 tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    34021 gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctagtcatca
    34081 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    34141 tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggtgctc
    34201 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    34261 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca
    34321 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    34381 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    34441 gctggcgtct gattggcgtc gcagtagtgc tggtggcggc gttcattccg attctgtggt
    34501 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctagac ccggaatcag
    34561 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    34621 tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc cccgaacgcc
    34681 ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    34741 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    34801 ccacctttgg tcgcgtcatg gctggtggct taatgctgat attattcgtt tatgtcttcg
    34861 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    34921 gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    34981 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    35041 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    35101 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    35161 gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    35221 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    35281 tatgatgccg aacccggcag taacctgacg gcctctggcg aagctttcga tccgacacag
    35341 ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    35401 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt
    35461 atttcacttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    35521 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    35581 acagtcgcca aacagactta cgccctgcca gcacctcccg atttaagcgg tggcgcggga
    35641 acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa
    35701 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc tccaacgacc
    35761 ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca
    35821 gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    35881 agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa gcgatcaggc tcgtgcgcaa
    35941 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    36001 gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag taccttgcag
    36061 caacgtttgc aaaccgaagc ccaattacag tcatttatta ctaccgcgca gtagcgtaaa
    36121 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    36181 atgcctgccg gtagtgcatt tgctatagta gggcactttt ttaattccat cacggatgtc
    36241 gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct ggcgctcacc
    36301 acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa tatcaaaact
    36361 atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga ctataactcc
    36421 ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgccag cctgaccaaa
    36481 atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa agaaactgat
    36541 ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa aggttcttcg
    36601 ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg cggtattaac
    36661 ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg tagccaggac
    36721 gcttttgttg gcttgatgaa cagctacgtt aacgcactgg gcctgaaaaa tacccacttc
    36781 cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga tatggcgctg
    36841 atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa agaaaaagaa
    36901 tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga taacagcctg
    36961 aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct tgttgcttct
    37021 gcgactgaag gccagatgcg cttgatttct gcggtgatgg gcggacgtac ttttaaaggc
    37081 cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga aaccgttaac
    37141 ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga ttctgatcgc
    37201 gcttcgttag gggttgataa agacgtgtac ctgaccattc cacgtggtcg catgaaagat
    37261 ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca aaagaatcag
    37321 gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt
    37381 gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta cattaaatta
    37441 atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt ccgtccccat
    37501 atactaagca tcagtaaaaa actcccgcct tctggcggga gttgctattt aattacgtta
    37561 cgccggagct gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac
    37621 ttacaaagtt atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca
    37681 gcgccatgcg ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca
    37741 ctcggtatct atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga
    37801 actaggcaaa atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggccc
    37861 ctggtcgggt aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc
    37921 tccacggaga tgcagttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc
    37981 cttacgagcc aatctcccag gctatgcatg aattcaccga tacccgcgat gatagtaccc
    38041 ttgatgaaat ctggctggtc gagcactatc cggtattcac ccaaggtcag gcaggaaaag
    38101 cggagcacat tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg
    38161 tgacttatca cgggccgggg caacaggtga tgtatgtgtt gcttaacctg aaacgccgta
    38221 aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg
    38281 aactgggtat agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga
    38341 aaatttgctc actgggttta cgtattcgac gcggttgttc attccacggt ctggcattaa
    38401 acgtcaatat ggatctttca ccatttttac gtattaatcc ttgtgggtat gccggaatgg
    38461 aaatggctaa aatatcacaa tggaaacccg aagcgacgac taataatatt gcaccacgtt
    38521 tactggaaaa tattttagcg ctactaaaca atcctgactt cgaatatatt accgcttaat
    38581 tgcatacctc aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt
    38641 acatctcctt cgcgtttgta tactttcttt atcacttgca gaaatgcggt gctacgcctt
    38701 gctccgtaac tcaccccatt gattaaccta ttgaaatagt tgattatggt gagcaaaagc
    38761 gttccgttcc aattagacat aagattattg taattttttt aaatattatt tttccatgaa
    38821 taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt aagcagaaag
    38881 tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct taaccttctg
    38941 actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa agtgcttaat
    39001 ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt ccctgaccca
    39061 ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca tctacatgag
    39121 tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg aagctatgat
    39181 aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt ccttcctgtc
    39241 atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc gcccatcagc
    39301 gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa catgttttgc
    39361 accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt aatttgccgc
    39421 gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa cgctgcgcat
    39481 gtactcctgt taccggaagg gcaaaatttc agcggtctgc ggcagagagt tcaagagatg
    39541 tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc tgcactggtt
    39601 gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt tagtcgttgc
    39661 tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga tttctctatc
    39721 cactacaaca aattcagcct gcgtgatccc atcctgcacg gggtaataga tgttatccgt
    39781 aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat aaaatggcac
    39841 aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt ccgtttcaat
    39901 gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct attgaattga
    39961 aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac ccattgtgat
    40021 ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg ttaaaaacgt
    40081 ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
    40141 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt
    40201 ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt
    40261 tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg
    40321 cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg ccgatatggc
    40381 gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca
    40441 gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac
    40501 tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
    40561 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc
    40621 tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga
    40681 aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag aaattattga
    40741 ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc aatatttgca
    40801 gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt tcgacgaaat
    40861 gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt ttgtccgctc
    40921 ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
    40981 cttacatctg tctgataaaa aacccgcttt ttg
//
LOCUS       DAEEBB010000016        40926 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEEBB010000016 DAEEBB010000000
VERSION     DAEEBB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.016"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..789
                     /locus_tag="KLF07_000861"
     CDS             <1..789
                     /locus_tag="KLF07_000861"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBD9830952.1"
                     /translation="LITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNL
                     LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTY
                     PLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMN
                     PISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDL
                     TNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            791..1027
                     /locus_tag="KLF07_000862"
     CDS             791..1027
                     /locus_tag="KLF07_000862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9830953.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1200..1875)
                     /locus_tag="KLF07_000863"
                     /pseudo
     CDS             complement(1200..1875)
                     /locus_tag="KLF07_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2020..2628)
                     /locus_tag="KLF07_000864"
     CDS             complement(2020..2628)
                     /locus_tag="KLF07_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBD9830954.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2812..3129)
                     /gene="metJ"
                     /locus_tag="KLF07_000865"
     CDS             complement(2812..3129)
                     /gene="metJ"
                     /locus_tag="KLF07_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBD9830955.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3406..4566
                     /gene="metB"
                     /locus_tag="KLF07_000866"
     CDS             3406..4566
                     /gene="metB"
                     /locus_tag="KLF07_000866"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBD9830956.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4569..7001
                     /gene="metL"
                     /locus_tag="KLF07_000867"
     CDS             4569..7001
                     /gene="metL"
                     /locus_tag="KLF07_000867"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBD9830957.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7350..8240
                     /gene="metF"
                     /locus_tag="KLF07_000868"
     CDS             7350..8240
                     /gene="metF"
                     /locus_tag="KLF07_000868"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBD9830958.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8569..10749
                     /gene="katG"
                     /locus_tag="KLF07_000869"
     CDS             8569..10749
                     /gene="katG"
                     /locus_tag="KLF07_000869"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBD9830959.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10843..11748
                     /gene="yijE"
                     /locus_tag="KLF07_000870"
     CDS             10843..11748
                     /gene="yijE"
                     /locus_tag="KLF07_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBD9830960.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11775..12392)
                     /locus_tag="KLF07_000871"
     CDS             complement(11775..12392)
                     /locus_tag="KLF07_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBD9830961.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12666..13769)
                     /gene="gldA"
                     /locus_tag="KLF07_000872"
     CDS             complement(12666..13769)
                     /gene="gldA"
                     /locus_tag="KLF07_000872"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBD9830962.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13780..14442)
                     /gene="fsa"
                     /locus_tag="KLF07_000873"
     CDS             complement(13780..14442)
                     /gene="fsa"
                     /locus_tag="KLF07_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBD9830963.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14454..16955)
                     /gene="ptsP"
                     /locus_tag="KLF07_000874"
     CDS             complement(14454..16955)
                     /gene="ptsP"
                     /locus_tag="KLF07_000874"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD9830964.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17264..18343
                     /locus_tag="KLF07_000875"
     CDS             17264..18343
                     /locus_tag="KLF07_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBD9830965.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18358..18678
                     /locus_tag="KLF07_000876"
                     /pseudo
     CDS             18358..18678
                     /locus_tag="KLF07_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18729..21025
                     /locus_tag="KLF07_000877"
                     /pseudo
     CDS             18729..21025
                     /locus_tag="KLF07_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20991..21869
                     /locus_tag="KLF07_000878"
     CDS             20991..21869
                     /locus_tag="KLF07_000878"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBD9830966.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21871..22212
                     /locus_tag="KLF07_000879"
     CDS             21871..22212
                     /locus_tag="KLF07_000879"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD9830967.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22199..23050)
                     /locus_tag="KLF07_000880"
     CDS             complement(22199..23050)
                     /locus_tag="KLF07_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9830968.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23265..24998)
                     /locus_tag="KLF07_000881"
     CDS             complement(23265..24998)
                     /locus_tag="KLF07_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBD9830969.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25181..27832)
                     /gene="ppc"
                     /locus_tag="KLF07_000882"
     CDS             complement(25181..27832)
                     /gene="ppc"
                     /locus_tag="KLF07_000882"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBD9830970.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28184..29335)
                     /gene="argE"
                     /locus_tag="KLF07_000883"
     CDS             complement(28184..29335)
                     /gene="argE"
                     /locus_tag="KLF07_000883"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBD9830971.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29489..30493
                     /gene="argC"
                     /locus_tag="KLF07_000884"
     CDS             29489..30493
                     /gene="argC"
                     /locus_tag="KLF07_000884"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBD9830972.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30501..31277
                     /gene="argB"
                     /locus_tag="KLF07_000885"
     CDS             30501..31277
                     /gene="argB"
                     /locus_tag="KLF07_000885"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBD9830973.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31338..32711
                     /gene="argH"
                     /locus_tag="KLF07_000886"
     CDS             31338..32711
                     /gene="argH"
                     /locus_tag="KLF07_000886"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBD9830974.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32978..33895
                     /gene="oxyR"
                     /locus_tag="KLF07_000887"
     CDS             32978..33895
                     /gene="oxyR"
                     /locus_tag="KLF07_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBD9830975.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33878..35278)
                     /gene="sthA"
                     /locus_tag="KLF07_000888"
     CDS             complement(33878..35278)
                     /gene="sthA"
                     /locus_tag="KLF07_000888"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBD9830976.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35599..36249
                     /gene="fabR"
                     /locus_tag="KLF07_000889"
     CDS             35599..36249
                     /gene="fabR"
                     /locus_tag="KLF07_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBD9830977.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36249..36608
                     /locus_tag="KLF07_000890"
     CDS             36249..36608
                     /locus_tag="KLF07_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBD9830978.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36648..37748)
                     /gene="trmA"
                     /locus_tag="KLF07_000891"
     CDS             complement(36648..37748)
                     /gene="trmA"
                     /locus_tag="KLF07_000891"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBD9830979.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37862..38052
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38117..39961
                     /gene="btuB"
                     /locus_tag="KLF07_000892"
     CDS             38117..39961
                     /gene="btuB"
                     /locus_tag="KLF07_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBD9830980.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39906..40763
                     /gene="murI"
                     /locus_tag="KLF07_000893"
     CDS             39906..40763
                     /gene="murI"
                     /locus_tag="KLF07_000893"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD9830981.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9459 a        10199 c        11240 g        10028 t
ORIGIN      
        1 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
       61 agccagttaa cgtcagccac cgggcctgac gggctggaaa tacgccggga atatgatgaa
      121 tggggcaacc tgctgaatga agagaacccg catcagctgc agcagcttat tcgtttgccg
      181 gggcagcagt atgatgagga gtccggcctg tattataacc gccaccgcta ttatgacccg
      241 ctgcaggggc ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat
      301 acatatccgc tgagcccggt gaatagcatg gatccattag gattatatga atttaaatca
      361 aaaaatattg atgatattgg aatatttgca ttggcaatgt gtaatggaga atcaattaac
      421 gagaataaag aatatggtgg actaatatgt aagaagcaag gtgaatattt ccccatgaat
      481 ccgataagtt caaatgataa tgatagtgta gacttgcgaa atataaaatg ccctgaaggt
      541 tcagagagag taggcgatta tcacactcac ggtttttact ctgacgataa aggaaataaa
      601 gtaacaaaag aaaatgatgt ttatgatagt ctaaattttt caagcaaaga tttaacgaat
      661 tcttatatga atggaatggg aaaaaaagaa tacagtagtt acttgggaac accaaataac
      721 acctatctaa aatataatcc caaagctaaa gggaatggag ttacaattat caggcaaggg
      781 agtaattaaa atgaaggcaa tacaatgttg ttatttcctt gctttagtct ccagtcattt
      841 aatggctgga gttatgggtg atgataatat atttttaagt aattcggatt cagctgcttt
      901 atattgtgtg gctaaaatat ttccagatgg agaaaatatt ttaatatata aagtaaatat
      961 aattaatgct agttcaaaaa aagatgtttt ttttatgaaa gcccaacatc aacccacaat
     1021 gacataatgg ggggcggggg aatgtcatat gcctatgaat atgatgtaaa atataatata
     1081 tgtcatttga gaaaaaagag cagaatgagg taaaaatctt tggctgtgta ttcagtctgg
     1141 taatcatcca aggaaagctt atcagaaggg tgatgactcc aattagtaga acatatattt
     1201 tcgataaaag caccgatgac tttttcatga agttctacag aacgcgagaa acagatggtt
     1261 ctgcgggcca gacgtttgat tcgggttctc agggtcagat tgttacgctc aatgtgctga
     1321 gtaaaaatct taccagtcag atgcttctcc ttcggtaact ctctagcgta actaccccat
     1381 tcgtcagtgg tcaacatgcc gatggtaaaa gggtaagcag cgcccgcaat tcacgacagg
     1441 tttcgtcagt ccgggggcca aaagtgtagg ccagaacacc accagttttc gtgttgtaca
     1501 cgtaccagag ccagtgctgt cgcgctttac tgccgacaaa gctccattgc tgactaagtt
     1561 tgcagatgag tgccacatca gcatgagcga ccggggatga cgttattctt cgtggcgaga
     1621 gttttttaaa gttcggatga ctgtattaat gccgattttc agcgtacggg cagtatcgcg
     1681 aaccccggca ccgttgaaag tcatttcagc gatttggtct ttaactcccg gtttacgggg
     1741 ctcgtaacag taactgagct gaaatacccg gtgacagtca cggccgcgga atcgctcatg
     1801 tctttttggg ttctgcccgt gacggtaaac ctgaacggac tggcaacggg ggcaatgaag
     1861 gttaactgtg accatgaaac aaccttaaaa gcgagtatta tatccttaat tcaactaatt
     1921 ggaggcatca ccgtttaccg gagcgacagc ttgcggaaag gtcgtgcaac tacaaagcaa
     1981 caaacagact acggcgggaa ttattttttt catcatccat taccacgaaa acagcggcca
     2041 ctctttggcg actgtggtga gttgtggcgc gtcgaaaacg gcttgtggtg aaactaccgt
     2101 ttcctcaagc ggaatatttt tgccgtagcg ttctttgagc ttagcctgga ccagcgggtt
     2161 gctgagatcg aactctttca acttctgctg ctcgcccacg cgcattccca gcgcgttctg
     2221 gtaaaccttc cacaccactt ccgaacagta ctggcgatcg tcgctccatg agaagctgaa
     2281 atcatacggt ttaccgagat aacgttttgc cgtttgcgcc agtttttgct gttgttcaac
     2341 actcagtccg ccttcaacgc ggcgaacaac gtatttgccc ttttcaccat gggcgatcca
     2401 ctgcttgagc ggggggtatt tcaccgggcc gaccgcttca aaaacgtagg gctttttgtt
     2461 gcgtatcacc agcataccgg tgtggctata atcggaatgg gtcgccagtt ggatcgcttt
     2521 actttgcgat gagcgagaga tctgaaagat gatgtcgccg gtttgtggct gccaggcaaa
     2581 ggcaggtaca gaaaccagca ggctgaggat cagcagcctg tttttcatag ttaaacgtcc
     2641 atgtataaaa agcggtgggt cgcagacaac gtgctcgttg tttatgccgg atgcggcgtg
     2701 aacgccttat ccggcctaca agttcgtgca aattcaataa attgcaatat gacgtaggcc
     2761 tgataagcgt agcgcatcag gcgattccac tccgcgccgc tcttttttgc tttagtattc
     2821 ccacgtctcc gggttaatcc ccatctcacg catgatctct tttgccgctt ccgggatttc
     2881 gtcgctgcgc tctttacgca gatcggcatc atccggcaaa ggttgcccgg taaaggcatg
     2941 cagaaacgct tcgcacagca gctcgctgtt ggtagcgtga cgcaggttgt tcacctgacg
     3001 acgcgtgcgt tcatcggtga ggatttttaa caccttaaga ggaatggaaa ccgtaatctt
     3061 tttgacttgt tcactcttct tgccgtgctc agcgtatggg ctgatatatt cgccgctcca
     3121 ttcagccatg agatacttaa tcctcttcgt caataaattg agaccagacc acacaattta
     3181 tgtgggtact gaccgtaaac ccgcatagtt taccgtacag gcgttaccgt gacatcgtgt
     3241 aatgcacctg tcggcgtgat aatgcatata attttaacgg ctatttggga tttgctcaat
     3301 ctatacgcaa agaagtttag atgtccagat gtattgacgt ccattaacac aatgtttact
     3361 ctggtgcctg acatttcacc gacaaagccc aggaaacttc atcacatgac gcgtaaacag
     3421 gccaccatcg cagtgcgtag cgggttaaat gacgacgaac agtatggttg cgttgtccct
     3481 cccatccatc tttccagcac ctataacttt accggattta atgaaccgcg cgcgcatgat
     3541 tactcgcgtc gcggcaaccc aacgcgcgat gtggttcagc gtgcgctggc agaactggaa
     3601 ggcggtgctg gcgcagtact tactaatacc ggcatgtccg cgattcacct ggtaacgacc
     3661 gtctttttga aacctggcga tctgctggtt gcgccgcacg actgctacgg cggtagctat
     3721 cgcctgttcg acagtctggc gaaacgcggt tgctatcgcg tgttgtttgt tgatcaaggc
     3781 gatgaacagg cattacgggc agcgctggca gaaaaaccca aactggtact ggtagaaagc
     3841 ccaagtaatc cattgttacg cgtcgtggat attgcgaaaa tctgccatct ggcaagggaa
     3901 gtcggggcgg tgagcgtggt ggataacacc ttcttaagcc cggcattaca aaatccgctg
     3961 gcattaggtg ccgatctggt gttgcattca tgcacgaaat atctgaacgg tcactctgac
     4021 gtggtggctg gcgtggtgat tgctaaagac ccggacgttg tcactgaact tgcctggtgg
     4081 gcaaacaata ttggcgtgac gggcggcgcg tttgacagct atctgctgct acgcgggttg
     4141 cgaacgctgg tgccgcgtat ggagctggcg cagcgcaacg cgcaggcgat tgtgaaatat
     4201 ctgcaaaccc agccgttggt gaaaaaactg tatcacccgt cgctgccgga aaatcagggg
     4261 catgaaattg ccgcgcgtca gcaaaaaggc tttggcgcaa tgttgagttt tgaactggat
     4321 ggcgatgagc agacgctgcg tcgtttcctg agcgggctgt cgttgtttac gctggcggaa
     4381 tcattagggg gagtggaaag tttaatctct cacgccgcaa ccatgacaca tgcaggcatg
     4441 gcaccagaag cgcgtgctgc cgccgggatc tccgagacgc tgctgcgtat ctccaccggt
     4501 attgaagatg gcgaagattt aattgccgac ctggaaaatg gcttccgggc tgcaaacaag
     4561 gggtaaaaat gagtgtgatt gcgcaggcag gggcgaaagg tcgtcagctg cataaatttg
     4621 gtggcagtag tctggctgat gtgaagtgtt atttgcgtgt cgcgggcatt atggcggagt
     4681 actctcagcc tgacgatatg atggtggttt ccgccgccgg tagcaccact aaccagttga
     4741 ttaactggtt gaaactaagc cagaccgatc gtctctctgc gcatcaggtt caacaaacgc
     4801 tgcgtcgcta tcagtgcgat ctgattagcg gtctgctacc cgctgaagaa gccgatagcc
     4861 tcattagcgc ttttgtcagc gatctggaac gcctggcggc gctgctcgac agcggtatta
     4921 acgacgcagt gtatgcggaa gtggtgggcc acggggaagt atggtcggca cgcctgatgt
     4981 ctgcggtact taatcaacaa gggctgccag cggcctggct tgatgcccgc gagtttttac
     5041 gcgctgaacg cgccgcacaa ccgcaggttg atgaagggct ttcttacccg ttgctgcaac
     5101 agctactggt gcaacatccg ggcaaacgtc tggtggtgac cgggtttatc agccgtaaca
     5161 acgccggtga aacggtgctg ctggggcgta acggttccga ctattccgcg acacaaatcg
     5221 gtgcgctggc gggtgtttct cgtgtaacca tctggagcga cgtcgccggg gtatacagtg
     5281 ccgacccgcg taaagtgaaa gatgcctgtc tgctgccgtt gctgcgtctg gatgaagcca
     5341 gcgaactggc tcgtctggcg gctcccgttc ttcacgcccg tactttacag ccggtttctg
     5401 gcagcgaaat cgacctgcaa ctgcgctgta gctacacgcc ggatcaaggt tccacgcgca
     5461 ttgaacgcgt gctggcctcc ggtactggtg cgcgtattgt caccagccac gatgatgtct
     5521 gtttgattga gtttcaggtg cccgccagtc aggatttcaa actggcgcat aaagagatcg
     5581 accaaatcct gaaacgcgcg caggtacgcc cgctggcggt tggcgtacat aacgatcgcc
     5641 agttgctgca attttgctac acctcagaag tggccgacag tgcgctgaaa atcctcgacg
     5701 aagcggggtt acctggcgaa ctgcgcctgc gtcaggggct ggcgctggtg gcgatggtcg
     5761 gtgcaggcgt cacccgtaac ccgctgcatt gccaccgctt ctggcagcaa ctgaaaggcc
     5821 agccggtcga atttacctgg cagtccgatg acggcatcag cctggtggca gtactgcgca
     5881 ccggcccgac cgaaagcctg attcaggggc tgcatcagtc cgtcttccgc gcagaaaaac
     5941 gcatcggcct ggtattgttc ggtaagggca atatcggttc ccgttggctg gaactgttcg
     6001 cccgtgagca gagcacgctt tcggcacgta ccgactttga atttgtgctg gcaggcgtgg
     6061 tggacagccg ccgcagtctg ttgagctatg acgggctgga cgccagccgc gcgttagcct
     6121 tcttcaacga tgaagcggtt gagcaggatg aagagtcgtt gttcctgtgg atgcgcgccc
     6181 atccgtatga tgatttagtg gtgctggacg ttaccgccag ccagcagctt gctgatcagt
     6241 atcttgattt cgccagccac ggtttccacg tcatcagcgc caacaaactg gcgggagcca
     6301 gcgacagcaa taaatatcgc cagatccacg acgccttcga aaaaaccggg cgtcactggc
     6361 tgtacaatgc caccgtcggt gcgggcttac cgatcaacca caccgtgcgc gatctgatcg
     6421 acagcggcga tactattttg tcgatcagcg ggatcttctc cggcacgctc tcctggttgt
     6481 tcctgcaatt cgacggtagc gtgccgttta ccgagctggt ggatcaggcg tggcagcagg
     6541 gcttaaccga acctgacccg cgtgacgatc tctctggcaa agacgtgatg cgcaagctgg
     6601 tgattctggc gcgtgaagca ggttacaaca tcgaaccgga tcaggtacgc gtggagtcgc
     6661 tggtgcctgc ccattgcgaa ggcggcagca tcgaccattt ctttgaaaat ggcgatgaac
     6721 ttaacgagca gatggtgcaa cggctggaag cggcccgcga aatggggctg gtgctgcgct
     6781 acgtggcgcg tttcgatgcc aatggcaaag cgcgtgtggg cgtggaagcg gtgcgtgaag
     6841 accatccgct ggcatcactg ctgccgtgcg ataacgtctt tgccatcgaa agccgctggt
     6901 atcgcgataa tccgctggtg atccgtgggc ctggcgctgg gcgcgacgtc accgccgggg
     6961 cgattcagtc ggatatcaac cggctggcac agttgttgta atttcagaaa tttaataatg
     7021 cccggtactc atgttttcgg gtttatggtt tctaatgaaa tatattgaat tatcatagga
     7081 ttaggccgga ttaagcgttt acgacgaatc cggcaagaag caataagtac atggttagtt
     7141 tatatttgca gtccggtttg ctttgcatac cggattttct ttttcttacc atcctgaagt
     7201 tttttcatct tccctgattt ttcctcacca tcattggtca tttttcggtt gacgcccttc
     7261 ggcttttcct tcatctttac atctggacgt ctaaacggat agatgtgcac aacacaacat
     7321 ataactacaa gcgattgatg aggtaaggta tgagcttttt tcacgccagc cagcgggatg
     7381 ccctgaatca gagcctggca gaagtccagg ggcagattaa cgtttcgttc gagtttttcc
     7441 cgccgcgtac cagtgaaatg gagcagaccc tgtggaactc catcgatcgc cttagcagcc
     7501 tgaaaccgaa gtttgtatcg gtgacctatg gcgcgaactc cggcgagcgc gaccgtacgc
     7561 acagcattat taaaggcatt aaagatcgca ctggtctgga agcggcaccg catcttactt
     7621 gcattgatgc gacgcccgac gagttgcgca ccattgcacg cgactactgg aataacggta
     7681 ttcgtcatat cgtggcgctg cgtggcgatc tgccgccggg aagtggtaag ccagaaatgt
     7741 atgcttctga cctggtgacg ctattaaaag aagtggcaga tttcgatatc tccgtggcgg
     7801 cgtatccgga agttcacccg gaagcaaaaa gcgctcaggc ggatttgctt aatctgaaac
     7861 gcaaagtcga tgccggagcc aaccgcgcga ttactcagtt cttcttcgat gtcgaaagct
     7921 acctgcgttt tcgtgaccgc tgtgtatcgg cgggcattga tgtggaaatt attccgggaa
     7981 ttttgccggt atctaacttt aaacaggcga agaaatttgc cgatatgacc aacgtgcgta
     8041 ttccggcgtg gatggcgcaa atgttcgacg gcctggatga tgatgccgaa acccgcaaac
     8101 tggtgggcgc gaatattgcc atggatatgg tgaagatttt aagccgtgaa ggagtgaaag
     8161 atttccactt ctatacgctt aaccgcgctg aaatgagtta cgcgatttgc catacgctgg
     8221 gtgttcgacc tggtttataa atagtgtggc ttttgtgaaa atcacacagt gatcacaaat
     8281 tttaaacaga gcacaaaatg ctgcctcgaa atgagggcgg gaaaataagg ttgtcagcct
     8341 tgttttctcc ctcattactt gaaggatatg aagctaaaac ccttttttat aaagcatttg
     8401 tccgaattcg gacataatca aaaaagctta attacgatca atttgaccta catctcttta
     8461 accaacaata tgtaagatct caactatcgc atccgtggat taattcaatt ataacttctc
     8521 tctaacgctg tgtatcgtaa cggtaacact gtagagggga gcacattgat gagcacgtca
     8581 gacgatatcc ataacacgac agccactggc aaatgcccgt tccatcaggg cggtcacgac
     8641 cagagcgcgg gggcgggcac aaccactcgc gactggtggc caaatcaact ccgtgttgac
     8701 ctgttaaacc aacattctaa tcgttctaac ccgctgggtg aggactttga ctaccgcaaa
     8761 gaattcagca aattagatta ctacggcctg aaaaaagatc tgaaagccct gctgacagaa
     8821 tctcagccgt ggtggccagc cgactggggc agctacgccg gtctgtttat tcgtatggcc
     8881 tggcacggcg caggtactta ccgttcaatc gatggacgcg gcggcgcggg tcgtggtcag
     8941 caacgttttg caccgctgaa ctcctggccg gataacgtaa gcctcgataa agcgcgtcgc
     9001 ctgttgtggc caatcaaaca gaaatatggt cagaaaatct cctgggccga cctgtttatc
     9061 ctcgcaggta acgtggcgct ggaaaactcc ggcttccgta ccttcggttt tggtgccggt
     9121 cgtgaagacg tctgggaacc ggatctggat gttaactggg gtgatgaaaa agcctggctg
     9181 actcaccgtc atccggaagc gctggcgaaa gcaccgctgg gtgcaaccga gatgggcctg
     9241 atttacgtta acccggaagg cccggatcac agcggcgaac cgctttctgc ggcagcagct
     9301 atccgcgcga ccttcggcaa catgggcatg aacgacgaag aaaccgtggc gctgattgcg
     9361 ggtggtcata cgctgggtaa aacccacggt gccggtccga catcaaacgt aggtcctgat
     9421 ccagaagctg caccgattga agaacaaggt ttaggctggg cgagcactta cggcagcggc
     9481 gttggcgcag atgccattac ctctggtctg gaagttgtct ggacccagac gccgacccag
     9541 tggagcaact atttcttcga aaacctgttc aaatatgagt gggtacagac ccgcagtccg
     9601 gctggcgcaa tccagttcga agcggtagac gcaccggaaa ttatcccgga tccattcgat
     9661 ccgtcgaaga aacgtaaacc gacgatgctg gtgaccgacc tgacgctgcg ttttgatcct
     9721 gagttcgaga agatctctcg tcgtttcctc aacgatccgc aggcgttcaa cgaagccttt
     9781 gcccgtgcct ggttcaaact gacgcacagg gatatggggc cgaaatctcg ctacatcggg
     9841 ccggaagtgc cgaaagaaga tctgatctgg caagatccgc tgccgcagcc gatctacaac
     9901 ccgaccgagc aggacattat cgatctgaaa ttcgcgattg cggattctgg tctgtctgtt
     9961 agtgagcggg tatcggtggc ctgggcatct gcttctacct tccgtggtgg cgacaaacgc
    10021 ggtggtgcca acggtgcgcg tctggcatta atgccgcagc gcgactggga tgtgaacgcc
    10081 gcagccgttc gtgctctgcc tgttctggag aaaatccaga aagagtctgg taaagcctcg
    10141 ctggcggata tcatcgtgct ggctggtgtg gttggtgttg agaaagccgc aagcgccgca
    10201 ggtttgagca ttcatgtacc gtttgcgccg ggccgcgttg atgcgcgtca ggatcagact
    10261 gacattgaga tgttcgagct gctggagcca attgctgacg gtttccgtaa ctatcgcgct
    10321 cgtctggatg tttccaccac cgagtcactg ctgatcgaca aagcccagca actgaccctg
    10381 accgcgccgg aaatgaccgc gctggtgggc gggatgcgcg tactgggtgc caacttcgat
    10441 ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat tgagcaatga cttcttcgtg
    10501 aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg aatcgaaaga gctgtttgaa
    10561 ggccgtgacc gcgaaaccgg cgaagtgaaa tacactgcca gccgtgcgga tctggtattt
    10621 ggttctaact ctgtcctgcg tgcggtggct gaagtttacg ccagcagcga tgctcacgag
    10681 aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga tgaacctcga ccgtttcgac
    10741 ctgctgtaat ctgaccttct tcagcgactg cctttcaggc agtcgctgaa gtttctttac
    10801 cggcgtatag tgtccacagg aaaactacac actggatctc tcatgtctgc cgcaggaaag
    10861 agcaacccac tggcaatcag tggcctggtt gtgctcacac ttatctggag ttatagctgg
    10921 attttcatga agcaagtcac cagctacatc ggtgccttcg actttaccgc cttacgctgc
    10981 attttcggcg ctctcgtttt attcatcgtc cttttattac gtggtcgcgg aatgcgcccg
    11041 acaccgttta aatacacctt agccattgcc ctgttacaaa cctgcgggat ggttggcctg
    11101 gcgcagtggg cgctggtcag cggcggagcg gggaaggtgg cgatcctaag ctataccatg
    11161 ccgttctggg tggtgatttt cgccgcgttg tttctcggtg aacgcctgcg acgtgggcaa
    11221 tatttcgcga tactgattgc cgctttcggc ttatttttgg tgttgcagcc gtggcaactc
    11281 gatttctctt cgatgaaaag tgccatgctg gcaatcctct ccggcgtcag ttggggggcg
    11341 agcgcgattg ttgctaaacg tctgtatgcc cgtcatccgc gcgtggattt attgtcgtta
    11401 acatcctggc agatgctcta tgcggcgctg gtgatgagtg tggtcgcttt actggtgccg
    11461 caacgtgaaa ttgactggca gcccaccgtg ttctgggcgc tggcctacag tgcgattctg
    11521 gcgacggcac tggcgtggag cttatggttg tttgtattga aaaacttgcc tgccagtatt
    11581 gccagcttaa gcacactggc cgttcccgtt tgcggcgtac tcttttcctg gtggctgctc
    11641 ggcgagaatc cgggggccgt tgaaggtagc ggtattgtgc tgattgtgat ggcactggcg
    11701 ctggtgagcc gtaagaaaaa agaagccgtt agtgtaaaaa ggatctgaat tttttcttca
    11761 tgtggggcga tcgcttattt aacaaaataa cgataatgcc ccaccatgcg ccagctaaac
    11821 agcacatctt cttcctgcgc acctgcgcca atgttatgta tcaccagcgg cgtaccgtcg
    11881 cgggcgaagc catctgaaac caccccaata tgtgccagcc cgttatctaa tcgccaggag
    11941 acaatatcgc ccgcttgata gtcactgggg ttcttgctga tggggcgtgt tttatcgtgg
    12001 tgggtaaacc aggtttccag attaggcacc cgacggtgat cgatgttgct gtccgggcgc
    12061 tttaactgcc atttttgcgg gtactcggca aaattcttcg ccatatcttc gtgcaccagt
    12121 ttctgcaaat cgaccttctg gctgcgcaat gcacggatca ccacatcgga acataccccg
    12181 cgtttttgcg ggacatcacc gccaggatag gtaagctgca tatacgccgg atcgtaaaat
    12241 agcgtgctgc cgatttgctg tctggcaccg tctgcgatgg caaggttggt attggcctgg
    12301 atttgtacca cggttggtgg aacggcggga gattttaagg agtggctggt aaatgccgtt
    12361 agcaggctga gcagcgccag tgaagctttc atctggttcg tccgtgaaaa taagagtcac
    12421 agattcgacc ttccgggata acgattgttc tgtagagaaa atgtccagaa gcggataaaa
    12481 aactcctgaa caacgtggga gtttcagatt actggcacag agtgccggat gcggcgtgaa
    12541 cgccttatct gtcctaaaaa aaaagaaaat tcaatatatt gcagagattg cgtaggcctg
    12601 ataagcgtag cgcatcaggc aattttgcgt tcaaactccc ggacaagccg ggagtttgga
    12661 gtaggttatt cccactcttg caggaaacgc tgaccgtact ggtcggctac cagcagagcg
    12721 gcgtaaacct gatctggcgt cgcgccgcca ggcatgttgt ggatggtttc acattctgca
    12781 catgccgctt cagccacaat tcgcattttc gccgggacat cttctttaat atccagttga
    12841 gcgagagtta ttggcaaacc taccgcatgg ctaagtgcag ctacggtttc gatttcctca
    12901 accggtgcgt tttccagaac cagctgcgtc agcgtaccaa atgccacttt ttcaccgtga
    12961 taatagtgat gcgcgtccgg gatagcggtc aggccgttat gcactgcgtg cgccgcagcc
    13021 agaccaccac tttcaaaacc aacaccgctc aaataggtgt tcgcttcaat cacgcgctcc
    13081 agcgccggag tcactacatg ctgttcggca gcaagcatcg ctttttcgcc ttcttccagc
    13141 agggtgttgt agcacagttc agccagtgcc agcgcagcct gggtgcactt gccgcccgcc
    13201 atggtggtcg cgccgctacg agagcaggca cgcgcttcaa accaggttgc cagcgcatcg
    13261 ccgatacccg ccgctaacag acgtgcaggt gcgccagcga cgattttggt gtcgacaatg
    13321 accatattcg ggttatttgg caacagcaga tagcggtcaa actcaccctc atcggtgtag
    13381 ataacagaca atgcgctgca cggtgcatcg gtagaggcga tagtcggtgc gatcgctacc
    13441 ggaacaccca tgaaatgtgc cagtgctttg gctgtatcga gggtttttcc gccaccgata
    13501 ccgagaattg cgccacactg cgcagtctcc gcgatgccac gcagacggtc gatctcattt
    13561 tgcgaacatc caccgccaaa cggcgcaatt tctactacca gtccagcatc tttaaagctt
    13621 ttctcgacag tggattgagc aaaacctaaa acaaatttgt cacccaccac taaccagcgt
    13681 tctgccagcg gcttcaggta ttcgcccaga cgattaatca catcagcgcc ctggatgtat
    13741 ttacccggtg attgaataat gcggtccata attgctcctt tagagatgag tagtgccaaa
    13801 tgcggcattc cagtcgtgtt cgaacttctc tatagctgac tctaccgcag gggtgttgag
    13861 catttgttgc gctacatcta agggcagggt gatggattca catcctgcca gtaaacagtc
    13921 cagcgcctga cgcggcgttt taaagctggc tgccagcacc atgctttctg gcgcgtgcat
    13981 ttctaacagc gcttgcagct cctgaaccgt acgaatgccg tctccgccct gggcatctac
    14041 gcggttaaca tacggagcaa cgtattttgc ccctgccagc gcggccagta acccttgtgc
    14101 agcactgtac actgcggtgc caagcgtagt aataccctct tttttcagta ttttaattgc
    14161 tgccagacct tcggaagtca ccgggatttt caccacaata cccggaatag cgtcgcgcag
    14221 gcgcttcgct tcttccacca tcccctgcgc gtcgcggctc atggtctgag caaacagaat
    14281 gccctcatca ccaatcgctt tttgcagacg cggcagcact tcccatatgg actccttgct
    14341 ggcagcgata atgctcgggt tagttgtcac cccggcaatg gggaatatgc gtgccagacg
    14401 ttcgacttct gcgacgttag cggtgtccag atacagttcc atgatgtttc cttttacagt
    14461 tccagttcat gttgcagcag gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg
    14521 ttacggaatt cttcgtgcat gatgcggcga gcgaggcgcg agaaaatgcg catgtgctga
    14581 tcgcccgcag cgtgtttgtt cagggttaac atgatgatga attgcgcttc atcatcaccc
    14641 cagcgcaccg gcgcttgcag acgcgcaacg ctgatggtgg attgctcaat gtgttctgat
    14701 ttgctgtgtg gaatggcaaa actaaagccc agaccggtag agaaaacggc ctcgcgcgcc
    14761 cacaagtcgg cttccagttt acgcggatag cggcagcgcc ctgccagcag caggttatcg
    14821 gtcatgcctt tgagcacttc ttctttgctg cgccagtcgc tttccagtgt gatgcactcg
    14881 gcggtgacca gcggtgcgtc ttgttgggtc atgcggaatt gcgccagcag gtgctccact
    14941 tccagcgaag tacggcaggc cattgcctgg ttgagcaact tgcggcactc acggctatca
    15001 agttgcgcca tccgcgcttt cgccgccgga attgatggtg cgctcatgct gagttcatcc
    15061 agccctaagc cgaccagcaa cggcagcacg gaacctttcg ctcccagctc accgcacaga
    15121 ccaatccatt taccctggcg atgcaccgcc tgcacggcgt aatcgagcgc ccgcaagaat
    15181 gccggattca ggctgttgta gtgacgagta accttagcgt tatcgcgatc cactgccagc
    15241 agatattgcg tcaggtcatt actaccaata ctaaagaaat caatctcttc gcagcattga
    15301 tcgatgatga acatcaccga cggcacttcc agcataatgc cgagctggat cttctcatca
    15361 aacggaatgt gttcgttacg tagttgctgt ttggcttctg ccagtttttc tttcacccat
    15421 aagatctctt ccattgagga gatcatcggg atcatgattt tcaggctgcc gtgagcggag
    15481 gcgcgcagga tcgagcgtag ttgtgtggta aataacgacg cgtattcttc ataaatacgc
    15541 acggcgcgat aaccgaggaa cgggtttgcc tctgcgggaa tgtttagata atcaacgggt
    15601 ttgtcgccgc caatgtccat agtgcgcaca ataatgctgc gtccgttggc agactccagc
    15661 gcctgacaaa aaatgttgta cagctcgctt tcgcccggtg cactggtgcg atccatatag
    15721 agcatttcag tgcggaacaa accaacgcct tccgcaccat tgccgaatgc ggcctgcgct
    15781 tccacggagt gagcgatgtt agcggcaatt tcaatgcgga taccgtcagc ggtacgggct
    15841 tgttgggtca gccagacacg ctgttgctca cgcagggcgt cctgtacgcg ggcttcttgc
    15901 tgataataac gagctacggc ttctcctggc tcaaccacaa tcgccccggc gttgccgtcg
    15961 atataaatcg tttgatgctg ccacggagtg agggcatcaa tatccacacc aaccagcgtc
    16021 ggaatgttga acgaacgggc aaggatcacc gtatgtgagg tggtgccgcc acttttcagc
    16081 aacagacctt tgaggtgatt tttatccagt tcgaggaact ggctgggggt cagttcatca
    16141 gccatacaaa tggcggactg cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag
    16201 atttgctgga gtaactggaa gcagacgtcg cgtacgtcca gggcacgttc ttgcaggtag
    16261 ctgctgctgg aacgggcgaa ctcttcgcaa aagtgattcg cactggcgac aatcgcttcg
    16321 gcgcagctta atccctcgct gacgcctgcc agtaagtgct gacgtaaaga tgtatcgcca
    16381 gccagggatc ggtgagcttc cagtatcgcg ctggtagcac cgtcgctatc cagcagacga
    16441 aactcaatgt ttttcagtac cagcgtcagg ccgttttcca gtgtggattg ctcggcgtca
    16501 acgtctttgg ctgcgggaag attactcagc gcattgagat ctaaagaaga gatcggcgtc
    16561 agaatgccgc ctgcactacc gctgcacacg gtgcgggcgc ggataatttg cggattcaga
    16621 ttggtcagtg aaaccggcag tggttccagt tcgtcagatt taacttccgc cagcggcgca
    16681 tcgcagtggg ggaattcatc gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg
    16741 tcggccccgg aaatcagtaa ctggcagtta tcgcccgcca gcgtatcggt gccaatcagc
    16801 gcaagggcgc ttttggcgtt gcccttgcgg tcagtgtgca ggttatgcca ctcaatttgt
    16861 gatgaaaaag tattacacag cgtttcaacg tggcttgccg gacgcgcatg tacgccgtta
    16921 ggtagctcac aaataaattc cacaatcagg gccattgcct ctctcccggt acggttttct
    16981 gtttacagga taagagcgca cggcaacggc ctgccatgtg acaaatctgc caaaagctgg
    17041 acaaatgtaa tgtaaccgtc aatttgcgac gcgtctcaca agacgccgtt ttgcggcatg
    17101 cttccggttt atcgcaagtt atgaggcgga tcgcattttt gtactgatat tacaaaagtc
    17161 cagtaaatgg cctttttatc cactgtttgc tccgcctgcg attgcctatt gtttcctcca
    17221 tcaaaaatat ggacatgggc tttcgcaagc ccgggagcag cttatgaatg agttggtgca
    17281 gatcctgaaa aatacccgtc agcatttaat gacgggcgtt tcacacatga ttcccttcgt
    17341 ggtatcgggc ggtattttgc tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc
    17401 ggatgccgta gccgatccaa atctgaaaaa actgtttgat atcggcgttg cgggcttgac
    17461 gctgatggtg cctttcctcg ccgcttacat tggttattcc attgcagaac gttctgcgct
    17521 ggctccatgc gctatcggta cctgggttgg taacagcttt ggtgcgggct tctttggtgc
    17581 actgatcgcc gggattatcg gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca
    17641 taaagttctg cgctcggtga tgcctatctt cattattcct atcgtcggca cactgattac
    17701 cgcaggcatc atgatgtggg gcttgggcga gcctgtaggg gcgttgacca acagcctgac
    17761 tcagtggctt caggggatgc agcagggcag cattgttatg ctggcggtga tcatgggtct
    17821 gatgctggcg ttcgatatgg gcggtccggt taacaaagtg gcctatgcct tcatgctgat
    17881 ttgcgttgct cagggtgttt ataccgtggt ggccatcgct gccgtgggta tttgtatccc
    17941 gccgctgggg atggggctgg cgacgctgat tggtcgtaaa aatttctccg cagaagagcg
    18001 cgaaaccggc aaagcggcac tggtgatggg ctgtgttggc gttactggag gggcgattcc
    18061 tttcgccgct gccgatccgc tgcgtgtcat tccttccatc atggtcggtt cagtttgtgg
    18121 tgcagtaact gcggcgctgg tcggtgcgca gtgctatgca ggctggggtg gtctgattgt
    18181 gctgccggtg gttgaaggca agctgggtta tatcgcagca gtggctgtcg gagcagtggt
    18241 gacggctgtt tgtgtgaacg tgctgaaaag tctggcgcgt aaaaatgggt cttcgactga
    18301 tgaaaaagaa gacgacctgg atttggattt tgaaattaat taattgagga acgagccatg
    18361 acgaaaatta ttgcagtaac cgcatgtcct tcaggtgttg cccataccta tatggcagca
    18421 gaggcgctgg aaagtgccgc gaaagcgaaa ggctgggaag tgaaagtaga aacctagggg
    18481 tcaattggtc tggaaaatga actgactgcg gaagatgtgg cgagcgctga tatggtgatt
    18541 ctgaccaaag atatcggcat caagtttgaa gagcgttttg ctggcaagac catcgtgcgc
    18601 gtcaacatca gcgatgcagt aaaacgtgct gacgccatta tgagcaaaat tgaagctcac
    18661 ctggcgcaaa ccgcttaagt tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg
    18721 agttcctcat gacgaatcgt atctctcgcc tcaaaactgc actgtttgcc aatacccgtg
    18781 aaatctcgct ggagcgggcg ctgctttata ccgccagcca tcggcaaacc gaaggcgaac
    18841 cggtgatatt gcgccgggcg aaagcaacag cgtatatcct tgaacatgtt gaaatttcga
    18901 ttcgtgatga agaactgatt gccggtaacc gcaccgtaaa accgcgcgcc gggattatgt
    18961 cgccggaaat ggacccttac tggctgctga aagagctgga tcaattcccg acgcgtccgc
    19021 aggaccgctt tgctatcagc gaagaagata aacgtatcta ccgtgaagag ttgttcccgt
    19081 actgggaaaa acgttcgatg aaagatttca tcaacgggca gatgacagat gaagtaaaag
    19141 ccgcgaccaa cacgcagatt ttcagcatca accagacgga taaaggccag gggcacatta
    19201 ttattgatta cccacgcctg ttgaatcacg gtctgggtga gctggtggca cagatgcagc
    19261 aacattgtca gcaacagccg gagaatcact tttatcaggc cgcgttgtta ctgctggaag
    19321 cctcgcagaa acacattttg cgttacgccg aactggcgga aacgatggcg gcaaactgca
    19381 cagatgcaca gcgtcgcgaa gaactgctga ctattgcgga gatctctcgc cataacgccg
    19441 aacataagcc gcagacgttc tggcaggcgt gccagttatt ctggtacatg aacatcattc
    19501 tgcaatacga atccaacgcc agttcgctgt ctttagggcg cttcgaccag tatatgttgc
    19561 cgttctatca ggcatcttta acccagggcg aagatccggc gttcctgaaa gaactgctcg
    19621 aatctttatg ggtgaaatgc aacgacatcg tgctgttgcg ctccaccagc agcgcgcgtt
    19681 atttcgcagg tttcccgacc ggctataccg cactgctcgg cgggttaacc gagaacggac
    19741 gtagcgcggt gaacgtgctt tcgttccttt gccttgacgc ctatcaaagc gtgcaattac
    19801 cgcaaccgaa cctcggcgtg cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa
    19861 ccgccgaaac cattcgcctc ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg
    19921 taccagcgtt cctcaaccgt ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg
    19981 gctgtgtgga attatctatt cccggcagaa cctacggctt gcatgacatc gcgatgttta
    20041 atctgctgaa agtgatggaa atctgcctgc atgaaaatga aggcaatgct gcgctgactt
    20101 atgaaggttt actggagcag atccgcgcca agatcagcca ctacatcacc ctgatggttg
    20161 agggcagcaa tatttgtgat atcggccatc gcgactgggc acctgtaccg ctgctctcgt
    20221 cttttatcag cgattgtctg gaaaaaggtc gcgatattac cgatggcggc gcgcgttata
    20281 acttctccgg cgtacagggg atcggtatcg ccaacctgag cgattctctc catgcgttga
    20341 aagggatggt ttttgagcaa cagcgtttaa gttttgacga attgctgtcg gtattaaaag
    20401 ccaactttgc aacgccagaa ggcgaaaaag tccgtgctcg cttaattaac cgcttcgaga
    20461 aatacggtaa cgatatcgac gaggtggata acatcagcgc cgaactgtta cgccactact
    20521 gcaaagaagt ggaaaaatac cagaacccgc gcgcggttac ttcacgccgg gatcgtatac
    20581 cgtttctgct cacgtcccgt tgggatcggt ggttggcgcg acgccagacg gtcgttttgc
    20641 cggggaacaa ctggcagacg gcggcttgtc acctatgctg ggccaggacg cacaagggcc
    20701 aacagcggta ctgaagtcag tcagtaagct cgataatacg ctgctgtcta acggtacgtt
    20761 gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa gcgggattac gcaaactggc
    20821 cgacttctta cgggcgttta cccagcttaa gttgcagcat attcagttta acgtggtgaa
    20881 cgccgacacg ttgcgggaag cgcaacagcg cccacaagat tatgccgggc tggtggtgcg
    20941 cgttgccgga tacagcgcct tctttgtcga actgtcgaag gagatccagg atgacatcat
    21001 ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg ccgcgatgat gtggcgcgca
    21061 ttttcaacat tcagcgttat tcactgaatg acggcgaggg cattcgtacg gtggtctttt
    21121 ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc ggagtcgatc tccggcaaaa
    21181 tccagacggt acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg cgtgatgcgg
    21241 atgaatgccc ctccggggcg tttgaacgga ttggtcgcga tatcagcctt gacgctctgg
    21301 aacgggaagt gatgaaagat gacattttct ttcgcacgtc cggcggcggc gtcacgcttt
    21361 ctggcggcga agtgttaatg caggcggagt ttgctacccg ttttttacag cgactgcggc
    21421 tgtggggtgt gtcatgcgcc attgaaactg ccggagacgc gccagcaagc aagctgttac
    21481 cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa aattatggac gcgactcagg
    21541 cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga gaatctgcgt ttgctggtga
    21601 gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc tggtttcacg ctcagcaggg
    21661 agaatatgca gcaggcgctg gatgtactga tcccgctgaa aatcaggcag atccatctgt
    21721 taccgtttca tcagtacggc gaaccgaaat accgcctgct ggggaaaaca tggtcgatga
    21781 aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat gcgcgaaatg gcagaacggg
    21841 ccggatttca ggttaccgtg ggaggttaaa atggcatacc tggtggcagt aaccgcctgc
    21901 gtaagcggcg tggcgcatac ttatatggcg gcggaacggc tggaaaagtt gtgccagtta
    21961 gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg gaacggagaa tcgtttagcg
    22021 gacgaggata tccgtcgggc ggatgttgct ctgttgatta cggatatcga gcttgccggt
    22081 gccgagcgat ttgaacattg ccgctatgtg caatgcagca tctacgcatt cctgcgtgag
    22141 ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg cgccgcagca aacccatctt
    22201 attctggagt agtcggtttt tctgtcagtt ggctgtggta ctgccggcga tattccgacg
    22261 gcgagcgttc ggtgttttta cgaaacagac ggcagaagta gttgctgtcg acaaagccgc
    22321 aggcgtgcgc cacttctttt accttcaggt catagccttt caataacgtc ttagcgtgct
    22381 ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc ccccgttttt tgaaacaggt
    22441 gcgagagata gtttggtgaa atatagaatg cctgtgcgac agattcgcgg gtaagcgcgg
    22501 aggcatagcg ttcgtcgata taatcgcgaa tagcttcaaa tagtgcctgg ctgcgtgagg
    22561 cggtctggat ttggctgccg agcaaatcgc ggcagtggct aagcaggctg gctacgataa
    22621 gccttgccgt ttgctgctcc tgcggctgca tttgcatttc attgagcgtt tgtagcagaa
    22681 aagaacctat acgtgggccg cgtcgggcga cgtgttgctt cgccagattt tgatattgtt
    22741 tgccatccca ttgcacgacg ctgaagccga gttgctgttt gccaaacagc acgctaaagg
    22801 tggtagcggg tgttttccat tgtgggaaat tccagccacc agcggcaacg tacagcacat
    22861 cgccgggaac taacgttgct ccagcgccgg tatcaacaaa ctcaccttcc agcacaattt
    22921 ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac cgggccgtta ctgctggcaa
    22981 agtaaatctg gcgcagggac agcgggccgt tgatcaggcg ggagagcaga tagctgacgc
    23041 cgtgatacat gttattccct taggtatgta gacgggaaaa cagtaactgg cattattaaa
    23101 aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg taaacattgc ggcagttgta
    23161 ttgccggatg tggcgtaaac gccttatcca gcctaccgcc gcgatctgta ggccggataa
    23221 gacgcgtcaa gcgtcgcatc cggcaaatag cgcctggctg ataattactg attacctacc
    23281 tggtcgccat acggcagagt gtcgtaatcg atcagcgcgt ttttcttgta cgggttacca
    23341 atccagcggg tagtttcttt gaactgcgga ttcaccactg aacgggttgg atcgtaaccg
    23401 tcgtaagata agcccgccaa atctgaccag gtgtggatca gttccgccag gctgtattta
    23461 cgatcgacat cctgtgagaa atcacgggga tgagtcgctt gccatttttc cgacgtccac
    23521 agcaggaacg gaatggtgta catgtggcgc gtcgggttgt cttcgttacg cccctgagtt
    23581 ttgtgcggcg gcgtgtcgta aacctcttca ccgtggtcag agaagtaaac caggaatccg
    23641 ttcgggtctg ccgctttaaa gtctttaatc aggctggcaa ccacatgatc gttatacagg
    23701 ttagcgttgt cataatcgtt atatgattcc agctcttctg cgcttaatcc tggcggaaca
    23761 tgatcggtat tgccatcaaa cttgccctga ttttccgggt agcggtattt gtatttgata
    23821 tgcgtaccca gcagatgaac gatgatcagt ttcttcggcg cagggtcatt cagcacatcc
    23881 tggaacggct tcagcacgtt ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc
    23941 atgtagtact gcttgtcggt ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc
    24001 atcgtctgct ggttggtgat ccagaaggtt ttataacccg cctgtttcat catgttcatc
    24061 agcgacggct gcgtcagata cagatccggg ttcttttcat tggcaaaggt cagcgcctgt
    24121 tgcaggattt caatggtgta cggacgagac gtaactacgt tattaaacac ggtcagattc
    24181 ggatcggttt tatgcagcgc atccagctcc ggcgtggttt cacgcggata accgtacaga
    24241 ctcatgcgtc cgcgctgggt cgactcgcca atcaccagca ctaaagtgcg cggttcgtta
    24301 cccgattcat ctttgaaatt agccagtggc ggcaaggcat tattttcatt cagtaacttt
    24361 gttagcgagt ttagttgctg acgatactga taatagccgg tcaggaattg ccacggtgcg
    24421 gcaggctcca tacgcgaggc caggttatcc aacgttttct caaacggctt gtttttgata
    24481 aacgtattca tggcgatcgg atgcagaatc aagccataaa gcagggcaaa agagacaaca
    24541 taacgccacg gctttggaat atagaccggg cgcaggcgtg tccacagcag aactgccacc
    24601 gccgtatagg ccagcgcgat aagcacaatt ttcaggctga aatactggct taaatactcg
    24661 ctggcttcgt tggtgttggt ttcgaacatc acaaacagaa cgctctgcga gaactcctgc
    24721 ccgtagatga cgtagtagca tagcgccgcc agagaggccg cccatagcac cacgccgatt
    24781 actgcggcaa taattttaat ccgcttcgga aagaggaata ccgggatcaa ccacagcgag
    24841 ctgaataaca gcgagtcgcg aatgccgttg gtgccactat aaccactgat gtaaataatg
    24901 gcctgtagca gagtagagaa aaaccaaaag tagagcagtg cccaacccag ggctttccag
    24961 ctaaaaaggg gtttagcctg gacttctgtg gaatgcatag taagaacctg tcttgaaaaa
    25021 aatatcgccg aatgtaacga caattcctta aggatatctg aaggtatatt cagaatttga
    25081 ataaaatgca gacagaaata tattgaaaac gagggtgtta gaacagaagt atttcagaaa
    25141 accctcgcgc aaaagcacga gggtttgcag aagaggaaga ttagccggta ttacgcatac
    25201 ctgccgcaat cccggcaata gtgaccatta acgcttgttc gacgcgagga tccggttcct
    25261 ggccttcttt ttctgcctgg cgggagcggt gcagcaactc ggcctgcaat acgttcagcg
    25321 ggtcggtgta aatattccgt agctgaatag actctgcaat ccacggcaga tcggccatca
    25381 gatgggaatc gttggcaatc gccagcacca ctttgatgtc ttcttcttgc aggttgcgta
    25441 actctttacc taacggccac agtgctttgt ctaccagacg ttgatcatag tattccgcca
    25501 accacaggtc tgctttggcg aagaccatct ccagcatgcc gagacgcgtc gagaagaatg
    25561 gccaatcgcg gcacatggct tccagctcgc tctgtttgcc atcttcgacc actttttgca
    25621 gcgccgtacc tgcacccagc caggcgggga gcatcaggcg gttttgcgtc caggcaaaaa
    25681 tccacggtat ggcgcgcagc gactcgacgc cgccggttgg gcgacgtttc gccggacgtg
    25741 aacccaacgg cagtttgccc aattcttgtt ccggcgtagc ggagcggaag taaggcacaa
    25801 aatctttgtt ttcacgtacg tagccacggt agagatcgca ggagatgact gacagttcat
    25861 ccataatgcg acgccagctc tctttcggct caggtggtgg cagcaggttg gcttccagaa
    25921 tcgccccggt gtaaagcgac aggctgctga cggtgatttc tggcagacca tatttaaagc
    25981 ggatcatctc gccctgttca gtcacgcgca ggccaccttt caggcttcct ggcggttgtg
    26041 acagcagcgc cgcatgggca ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga
    26101 acaacgtcag ctcaataccc gctttttcgc aggttttgat taatgcatcc tgtgcctgat
    26161 attgcgccca ggaagctgcc atcacgcccg catcttttgc tgagtcggaa tagccaatca
    26221 tcaccatctg tttgccctga atcaggccgc gataccagtc gatattgagc agctgggtca
    26281 tgacatcgtt ggcgttgttc aggtcatcga gggtttcaaa cagcggagca accggcatcg
    26341 caaacccgat acccgcttct ttcagcagca ggtggacagc cagtacgtcg gacggcgttt
    26401 tcgccatcga gatcacgtag gcggcaatgg agccttgcgg cgcttcggca atcacctggc
    26461 aggtatcgag cacttcgcgc gtttcggcgc ttggttgcca gttgcgcggc agaagcggac
    26521 gtttggagtt cagttcgcgg atcaggaacg cctgtttgtc ggcctctgac cagctttcgt
    26581 agtcgccgat accgaggtag cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct
    26641 cctgacggat atcaatacgg accagcggta cgccgaaaca tttcacgcgg cgcagggtgt
    26701 cgagcagatc gccgttggcg ataataccca tgccacacgc ctgaagtgac tggtagcaag
    26761 cgtagagcgg ttcccacagt tcttcgtttt gtgtcagcag cccttctggt tttggcagtt
    26821 cttcgccttt caggcgcgct tccagccatg cctgtgtcgc catcaggcga gaacgcaggt
    26881 ttttcatcag atagcgatac ggttctgcgg caccttcttc gccaaccagc gccagcagtt
    26941 caggggtcgc ttcaaccatc gacagttcag aaaccagaac ctgaatatct ttcaggaaca
    27001 gatcggtggc tttccagcga ctgagtagca ggacgtggcg ggtgatatcg gcagtgacgt
    27061 tcgggttgcc gtcgcggtca ccgcccatcc acgaagtaaa acggaccgga acaaattcga
    27121 cgggcagttt gtagccgagg ttctcttcca gttgttcgtt cagttcgcgc aagtaattcg
    27181 gtacgccttg ccacaggctg ttttctacta cggcaaagcc ccatttggct tcatctaccg
    27241 ggcttggacg cagcttacgg atttcatcag tatgccatga ctgggcgatt aactggcgca
    27301 ggcgacgcat cagctggttg tgttcgtagt cagcgatatc tttgttatcg agctgtttta
    27361 agcaggcgtt cacttccacc attttgtgga tcagtgtacg acgggtaatt tcggttgggt
    27421 gagccgtgag gaccagttcc agcgacagcg attccactgc ttttttgatg gtgtcttcgc
    27481 tcagttccgg ctggtttttc agtttgcgca gggtgcgggc gatcacttcc gggttgctgg
    27541 cagcttcgcc tttcggcgaa atgctgtggt attgctcggc ggtgttggcc aggttcagga
    27601 actgactaaa cgcacgcgca acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg
    27661 tgagcaactc ctggcggtta gcatcattgc cagcgcgtga agatttcgac aacttacgga
    27721 tagtttctac gcgttcaaga atgtgttctc ccaacgcatc cttgatggtt tctcccagca
    27781 ctttgccgag catactgaca ttactacgca atgcggaata ttgttcgttc atattacccc
    27841 agacacccca tcttatcgtt tgatagccct gtatccttca cgtcgcattg gcgcgaatat
    27901 gctcgggctt tgcttttcgt cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg
    27961 ctgcgaaatc gcttcagcaa acgaataaat agcaggaatt tacgccatta aattcacgac
    28021 gctttaaata agcgtaactt atggaaatgt taaaaaatcg ccatacataa caccaaaggt
    28081 gtaggtcgga taagatgcgc aagcatcgca tccgacatta ttgcggcacc ggagtttggc
    28141 aacggtgccg gatgcggcgc gagcgcctta tccggcctac gttttaatgc cagcaaaaat
    28201 ggtgaattac ctggataatc aactcacggg tgggtttaat aaaccgcgtc tccagatatt
    28261 catcaggctg atgagcttga ttgatggaac ccggaccgag caccagcgtc gggcataacg
    28321 tttgaataaa cggcgcttcg gtacagtaat tcaccacttc ggtttttgct ccgagcaatt
    28381 tctcaaccac ttcaaccagt tgatgattcg gtgggcattc atagccaggg atcggcggat
    28441 gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct caccggagcc aatgcatcgt
    28501 tgagcaaacc attaagttca ttgagtgtca tgctaggcag cggacgaata tccatatgca
    28561 actcacagca agcgcaaata cggttagaag cgtcgccacc gtgaatatgc ccgaggttga
    28621 gcgtagggta tggcacggta aatgcttcgt agtgataacg ttctttcagg ttatcgcgca
    28681 attgcaaaat atgcccgatg gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg
    28741 gatcgctgga gtgccccgac tggccctgaa tacggatggc gttagagata tgacctttat
    28801 gtgcgcgtac cggttgtagt gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg
    28861 cggtagtttc ggcaaaataa cgcgctccgg ccatactggt ttcttcatca gcagtcgcca
    28921 gaatgtagag cggttttttc agtttcgtga cgtcgacatc gcgtagcgta tcaaggataa
    28981 acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta aagcttgccg tcatgctccg
    29041 tcagtgtaaa cggatcgcgc gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc
    29101 ccgccagcaa caagccgcca gccccctgtc cgatacttgc cagcatattg aatttgttgc
    29161 gagttcctgg cacaggctgc acttccacat tgaagcctaa atctttaaac cagtccgcca
    29221 gcagagtgat taaatctgca ttgctttgat cgagtgcctc ttccgtggcg cttattgaag
    29281 gtgtggcaat cagagcacgg taaatctcga taaatggcgg taatttgttt ttcattgttg
    29341 acacacctct ggtcatgata gtatcaatat tcatgcagta tttatgaata aaaatacact
    29401 aacgttgagc gtaataaaac ccaccagccg taaggtgaat gttttacgtt taacctggca
    29461 accagacata agaaggtgaa tagccccgat gttgaatacg ctgattgtgg gtgccagcgg
    29521 ctacgctggc gcagagctag tgacctatgt aaatcgccat ccgcatatga acataaccgc
    29581 tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta atctccgatt tgcatccgca
    29641 gctaaaaggc atcgttgagc tgccgttgca gccgatgtcg gatatcagcg agtttagccc
    29701 aggggtggac gtagtgtttc tcgccaccgc ccatgaagtt agccacgatt tagcgccgca
    29761 gtttcttgaa gcgggctgcg tggtgttcga cctttccggt gcgtttcgtg ttaacgacgc
    29821 caccttctat gaaaaatatt acggctttac ccatcaatac ccggaactgc tggaacaggc
    29881 agcctacggt ctggcggagt ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt
    29941 gccaggctgt tatccgacgg cggcacagct ggcgctgaaa ccgttgattg atgccgatct
    30001 tcttgacctc aatcagtggc cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg
    30061 taaggcagcg atttcaaata gtttttgtga agttagcctg caaccgtatg gcgtctttac
    30121 tcatcgccat caaccagaga tcgccacaca cctcggtgct gacgttatct tcaccccaca
    30181 tctgggcaat ttcccgcgcg gcattctcga aaccattacc tgccgcctga aatcgggtgt
    30241 gacccaggcg caagtcgcgc aagtgttaca gcaggcgtat gcccataaac cgctggtgcg
    30301 gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat
    30361 cgggtttgcc gttcagggcg agcatctgat tattgtggcg accgaagaca acttactgaa
    30421 aggcgcggcg gcacaagcgg tacagtgcgc caatattcgt ttcggctatg cggaaacgca
    30481 gtctcttatt taagggtgca atgatgaatc cattaattat caaactgggc ggcgtactgc
    30541 tggatagtga agaggcgctg gaacgtctgt ttagcacact ggtgaattat cgtgagtcac
    30601 atcagcgtcc gctggtgatt gtgcacggcg gcggttgcgt ggtggatgag ctgatgaaag
    30661 ggctgaatct gccggtgaaa aagaaaaacg gcctgcgggt gacgcctgct gatcagatag
    30721 acattatcac cggagcactg gcgggaacgg caaataaaac cctgttggca tgggcgaaga
    30781 aacatcagat tgcggccgta ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc
    30841 agcttgatga agagttaggt catgttggac tggcgcagcc aggttcgcct aagcttatca
    30901 actccttgct ggagaacggt tatctgccgg tggtcagctc cattggcgta acagacgaag
    30961 ggcaactgat gaacgtcaat gccgaccagg cggcaacggc gctggcggca acgctgggcg
    31021 cggatctgat tttgctctcc gacgtcagcg gcattctcga cggcaaaggg caacgcattg
    31081 ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca gggcattatt actgacggca
    31141 tgatagtgaa agtgaacgcg gcgctggatg cggcccgcac gctgggccgt ccggtagata
    31201 tcgcctcctg gcgtcatgcg gagcagcttc cggcactgtt taacggtatg ccgatgggta
    31261 cgcggatttt agcttaagtt ttgttggccg gaggcgcagc tttccggcat tgaatttcaa
    31321 aataaggaaa cagagttatg gcactttggg gcgggcgttt tacccaggca gcagatcaac
    31381 ggttcaaaca attcaacgac tcactgcgct ttgattaccg tctggcggag caggatattg
    31441 ttggctctgt ggcctggtcc aaagccctgg tcacggtagg cgtgttaacc gcagaagagc
    31501 aggcgcaact ggaagaggcg ctgagcgtgc tgctggaaga tgttcgcgcc aggccacaac
    31561 aaatccttga aagcgacgcc gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca
    31621 aagtgggcca gttaggcaaa aagctgcata ccgggcgtag tcgtaacgat caggtagcga
    31681 ctgacctgaa actgtggtgc aaagataccg ttagcgagtt actgacggct aaccgacagc
    31741 tgcaatcggc gctggtggaa accgcacaaa acaatcagga cgcggtcatg ccaggttaca
    31801 ctcacctgca acgcgcccag ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga
    31861 tgctggcgcg tgatgaaagc cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat
    31921 taggctgtgg cgcgctggcg ggaacggcct atgaaatcga ccgtgaacag ttagcaggct
    31981 ggctgggctt tgcttcggcg acccgtaaca gtctggacag cgtttctgac cgtgaccatg
    32041 tgttggaact gctgtcggct gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag
    32101 atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga gctttctgac cgcgtgactt
    32161 ccggttcatc attaatgccg cagaagaaaa acccggatgc gctggagctg attcgcggta
    32221 aatgcggtcg ggtgcagggt gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc
    32281 tggcttacaa caaagatatg caggaagaca aagaaggtct gttcgacgcg ctcgatacct
    32341 ggctggactg cctgcatatg gcggcgctgg tgctggacgg cattcaggtg aaacgtccac
    32401 gttgccagga agcggctcag cagggttacg ccaacgccac cgaactggcg gattacctgg
    32461 tggcgaaagg cgtacctttc cgcgaggcgc accatatcgt gggtgaagcg gtggtggaag
    32521 ccattcgtca gggcaaaccg ctggaagatc tgccgctcga cgagttgcag aaattcagcc
    32581 cggtgattgg cgaagatgtc tatccgattt tgtcgctgca atcgtgcctc gacaagcgtg
    32641 cggcaaaagg cggcgtctca ccgcagcagg tggcccaagc gattgctttt gcgcaggcgc
    32701 ggttggggta agaacattta tatgtacaaa tttgagcctg gcttatcgcc gggctttttt
    32761 atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
    32821 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
    32881 tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg ctattctacc
    32941 tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta
    33001 cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagattcct gccacgttag
    33061 ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagctgggcg tgatgttgct
    33121 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
    33181 gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggcgagac
    33241 gatgtccgga ccgctgcata ttggtttgat tcccacggtt ggaccgtacc tgctaccgca
    33301 tattatcccg atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcaca
    33361 gacccaccag ttactggcgc aactggacag tggcaaactc gattgcgtga tcctcgcgct
    33421 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
    33481 tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ccgatagccg atctggcagg
    33541 ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg
    33601 ttttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg agacgctgcg
    33661 caacatggtg gcggcaggta gcgggatcac tttactgcca gcgctggctg tgccgccgga
    33721 gcgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat
    33781 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
    33841 catccgagca agaatggatg gctatttcga taaagtgtta aaacaggcgg tttaaaccgt
    33901 ttaacgcagc tacccgatag gcttccgcca tggtcgggta gttaaaggtg gtgttgacga
    33961 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
    34021 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
    34081 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcgcgtgcc agatgtttaa
    34141 actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt tcggttttgc
    34201 ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgcg
    34261 cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgctg
    34321 atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
    34381 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
    34441 acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag tcagctttca
    34501 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg atcttctcgt
    34561 actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag agagaatctg
    34621 acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta
    34681 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa
    34741 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat
    34801 gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt
    34861 cagcggttag tgtttcgacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa
    34921 caaagcgagc gttcccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca
    34981 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
    35041 ggagcagtcg cgaatggtcg ctgtatagtg ggttttgatt gaattctata atgcggctga
    35101 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
    35161 tttgataacg ctcgataact gcgacgcgca caccttgctt aaccaggccc attgcagcgc
    35221 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
    35281 tagggcttac ctgttcttat acataaaagc aacagaatgg taacattcta tcgcgggtaa
    35341 gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt
    35401 cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc
    35461 aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg ttaccgttca
    35521 ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt
    35581 ggcaaggatt cagacatcgt gatgggcata agagcgcaac aaaaagaaaa aacccgccgt
    35641 tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt
    35701 ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc
    35761 gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc
    35821 atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgcac ctcggtctcc
    35881 acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc
    35941 tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca cttcattgcg
    36001 gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa
    36061 gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc
    36121 gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg
    36181 gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag
    36241 gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg ttagttgctg
    36301 gcttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat
    36361 tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat cttaatcgca
    36421 ctatgccggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc gtactgctgt
    36481 acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac
    36541 tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg
    36601 ctgagtaatc ggtattatgc cggatgctgg cgcatccggc atgggtttta cttcgcggtc
    36661 agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt
    36721 tcgaccttgt gcgtctggct taatgtttcc agattcttgc ataacgtttc cgggttacag
    36781 gagatgtaca aaatacacgg atacgcctgc accattttct cggtttcact gtccagaccg
    36841 ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac tctttaagtc gatcccttgc
    36901 aggcggttaa actcgcgcac accattcatc gcctgagtaa attcttctgc cgccatacga
    36961 ataatttgta cgttatcaat atggttagct gcgatgttgt attgcgcagc agcaaccgac
    37021 ggcttagcga tttcggtggc taatacccga tcaaaattgc gcgccagcgc taatgaaaag
    37081 ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt tacgtccagc
    37141 gcccattcca gcatctgaat attcatcgcc gcgttcggct gggtaaagct gttttctacc
    37201 tgacggtaga tcatctcttt ccctgcgacc ggcagacgct cgtcgatgta atcttgatcc
    37261 agctcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgcgcgc
    37321 agtgcatcgc gcagggcctc cgcttcctga cgccactcat catccagctt cttatggtat
    37381 agcagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg
    37441 tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgata
    37501 agttcactgg cggcggggaa gctatccacg cggatacggc ttttggtttg ttgatcgaaa
    37561 atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcacg catccggtaa
    37621 tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt
    37681 tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg
    37741 ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag
    37801 atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa cttgttacaa
    37861 cctgtagcat ccacttgtcg gtcctgtgag ttaataggga atccagtgcg aatctggagc
    37921 tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg
    37981 ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa
    38041 cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta
    38101 ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt ctgtcacagc
    38161 cttttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg ctaaccgttt
    38221 tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga
    38281 ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac
    38341 ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca
    38401 tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg ggggtgagtg gttctgccga
    38461 ccttagccag ttccctattg cgcttgtcca gcgtgttgaa tatatccgtg ggccgcgctc
    38521 cgctgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga cgcgcgatga
    38581 acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc
    38641 tacgcagcaa caactggggg ataagacacg gggaacgctg ttgggcgatt atgcccatac
    38701 tcatggttat gatgttgttg cctatggtaa tacaggaacg caagcgcaga cagataacga
    38761 tggtttttta agtaaaacgc tttatggcgc gctggagcat aactttactg atgcctggag
    38821 cggctttgtg cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc
    38881 cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg
    38941 ctataacggc gaactgatta aatcacaact cattaccagc tatagccata gcaaagatta
    39001 caactacgat ccccattatg gtcgttatga ttcgtcggcg acgctcgatg agatgaagca
    39061 atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg gtgcgggtgt
    39121 cgactggcag aaacagacta cgacgccggg tacaggttat gttgaggatg gatatgatca
    39181 acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta cctttgaagg
    39241 cgcagcacgc agtgacgata actcacagtt tggtcgtcat ggaacctggc aaaccagcgc
    39301 cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat cttataaggc
    39361 accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc cggagaaaag
    39421 caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg
    39481 atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga aatattacaa
    39541 cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc aattttgata ccggaccact
    39601 gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg acacgccgtt
    39661 gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga
    39721 ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact catcttatcc
    39781 ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg cgtatccggt
    39841 cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac
    39901 agtctatggc taccaaactg caggacggga atacaccttg tctggcagct acaccttctg
    39961 aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg
    40021 agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc
    40081 cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt gtcaccgcgg
    40141 tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacactgcc agtaccgttt
    40201 cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta
    40261 aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag
    40321 ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa
    40381 tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctacatggc gaagatgttt
    40441 ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata
    40501 ccgttgtatt gggttgcacc catttccctc tactacaaga agaactgtta caagtgctgc
    40561 cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt
    40621 tagaacatga agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa
    40681 tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg
    40741 aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcagaaa attattttaa
    40801 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
    40861 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat ctcaacagag aaaagcaaaa
    40921 aaatgc
//
LOCUS       DAEEBB010000017        40803 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEEBB010000017 DAEEBB010000000
VERSION     DAEEBB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.017"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(62..138)
                     /locus_tag="KLF07_000894"
     tRNA            complement(62..138)
                     /locus_tag="KLF07_000894"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Met,seq:cat)
     gene            347..1444
                     /gene="mltA"
                     /locus_tag="KLF07_000895"
     CDS             347..1444
                     /gene="mltA"
                     /locus_tag="KLF07_000895"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBD9830982.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1521..2327
                     /gene="tcdA"
                     /locus_tag="KLF07_000896"
     CDS             1521..2327
                     /gene="tcdA"
                     /locus_tag="KLF07_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBD9830983.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2378..2821)
                     /gene="csdE"
                     /locus_tag="KLF07_000897"
     CDS             complement(2378..2821)
                     /gene="csdE"
                     /locus_tag="KLF07_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBD9830984.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2821..4026)
                     /gene="csdA"
                     /locus_tag="KLF07_000898"
     CDS             complement(2821..4026)
                     /gene="csdA"
                     /locus_tag="KLF07_000898"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBD9830985.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4218..4445
                     /locus_tag="KLF07_000899"
     CDS             4218..4445
                     /locus_tag="KLF07_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBD9830986.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4796..5713
                     /gene="gcvA"
                     /locus_tag="KLF07_000900"
     CDS             4796..5713
                     /gene="gcvA"
                     /locus_tag="KLF07_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBD9830987.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5732..6127
                     /locus_tag="KLF07_000901"
     CDS             5732..6127
                     /locus_tag="KLF07_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBD9830988.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6120..7220
                     /gene="rlmM"
                     /locus_tag="KLF07_000902"
     CDS             6120..7220
                     /gene="rlmM"
                     /locus_tag="KLF07_000902"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBD9830989.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7264..7995)
                     /gene="fucR"
                     /locus_tag="KLF07_000903"
     CDS             complement(7264..7995)
                     /gene="fucR"
                     /locus_tag="KLF07_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBD9830990.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8053..8475)
                     /gene="fucU"
                     /locus_tag="KLF07_000904"
     CDS             complement(8053..8475)
                     /gene="fucU"
                     /locus_tag="KLF07_000904"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBD9830991.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8477..9895)
                     /gene="fucK"
                     /locus_tag="KLF07_000905"
     CDS             complement(8477..9895)
                     /gene="fucK"
                     /locus_tag="KLF07_000905"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBD9830992.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10004..11779)
                     /gene="fucI"
                     /locus_tag="KLF07_000906"
                     /pseudo
     CDS             complement(10004..11779)
                     /gene="fucI"
                     /locus_tag="KLF07_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11812..13128)
                     /gene="fucP"
                     /locus_tag="KLF07_000907"
     CDS             complement(11812..13128)
                     /gene="fucP"
                     /locus_tag="KLF07_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBD9830993.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13675..14322
                     /gene="fucA"
                     /locus_tag="KLF07_000908"
     CDS             13675..14322
                     /gene="fucA"
                     /locus_tag="KLF07_000908"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBD9830994.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14350..15498
                     /gene="fucO"
                     /locus_tag="KLF07_000909"
     CDS             14350..15498
                     /gene="fucO"
                     /locus_tag="KLF07_000909"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBD9830995.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15603..16358)
                     /gene="xni"
                     /locus_tag="KLF07_000910"
     CDS             complement(15603..16358)
                     /gene="xni"
                     /locus_tag="KLF07_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBD9830996.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16470..17837)
                     /gene="sdaB"
                     /locus_tag="KLF07_000911"
     CDS             complement(16470..17837)
                     /gene="sdaB"
                     /locus_tag="KLF07_000911"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBD9830997.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17895..19184)
                     /gene="sdaC"
                     /locus_tag="KLF07_000912"
     CDS             complement(17895..19184)
                     /gene="sdaC"
                     /locus_tag="KLF07_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBD9830998.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19740..21104)
                     /gene="ppnN"
                     /locus_tag="KLF07_000913"
                     /gene_synonym="ygdH"
     CDS             complement(19740..21104)
                     /gene="ppnN"
                     /locus_tag="KLF07_000913"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBD9830999.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21216..22064)
                     /gene="queF"
                     /locus_tag="KLF07_000914"
     CDS             complement(21216..22064)
                     /gene="queF"
                     /locus_tag="KLF07_000914"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBD9831000.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22132..22677
                     /gene="syd"
                     /locus_tag="KLF07_000915"
     CDS             22132..22677
                     /gene="syd"
                     /locus_tag="KLF07_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBD9831001.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23299..23628
                     /locus_tag="KLF07_000916"
     CDS             23299..23628
                     /locus_tag="KLF07_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBD9831002.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23628..24410
                     /gene="truC"
                     /locus_tag="KLF07_000917"
     CDS             23628..24410
                     /gene="truC"
                     /locus_tag="KLF07_000917"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBD9831003.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24428..24877
                     /locus_tag="KLF07_000918"
     CDS             24428..24877
                     /locus_tag="KLF07_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBD9831004.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25312..26664
                     /gene="gudP"
                     /locus_tag="KLF07_000919"
     CDS             25312..26664
                     /gene="gudP"
                     /locus_tag="KLF07_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBD9831005.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26666..28006
                     /locus_tag="KLF07_000920"
     CDS             26666..28006
                     /locus_tag="KLF07_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD9831006.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28027..29367
                     /gene="gudD"
                     /locus_tag="KLF07_000921"
     CDS             28027..29367
                     /gene="gudD"
                     /locus_tag="KLF07_000921"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD9831007.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29599..32355)
                     /gene="barA"
                     /locus_tag="KLF07_000922"
     CDS             complement(29599..32355)
                     /gene="barA"
                     /locus_tag="KLF07_000922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBD9831008.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32412..33713
                     /gene="rlmD"
                     /locus_tag="KLF07_000923"
     CDS             32412..33713
                     /gene="rlmD"
                     /locus_tag="KLF07_000923"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBD9831009.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33761..35995
                     /locus_tag="KLF07_000924"
     CDS             33761..35995
                     /locus_tag="KLF07_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBD9831010.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36073..36321
                     /gene="mazE"
                     /locus_tag="KLF07_000925"
     CDS             36073..36321
                     /gene="mazE"
                     /locus_tag="KLF07_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBD9831011.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36321..36656
                     /gene="mazF"
                     /locus_tag="KLF07_000926"
     CDS             36321..36656
                     /gene="mazF"
                     /locus_tag="KLF07_000926"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBD9831012.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36727..37518
                     /gene="mazG"
                     /locus_tag="KLF07_000927"
     CDS             36727..37518
                     /gene="mazG"
                     /locus_tag="KLF07_000927"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBD9831013.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37746..39383
                     /gene="pyrG"
                     /locus_tag="KLF07_000928"
     CDS             37746..39383
                     /gene="pyrG"
                     /locus_tag="KLF07_000928"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBD9831014.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39471..40769
                     /gene="eno"
                     /locus_tag="KLF07_000929"
     CDS             39471..40769
                     /gene="eno"
                     /locus_tag="KLF07_000929"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBD9831015.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10098 a         9886 c        10850 g         9969 t
ORIGIN      
        1 acgagctacc aggctgctcc accccgcgtc accgtactgc tttacttcat caaattttaa
       61 ttggttgcgg gggccggatt tgaaccgacg accttcgggt tatgagcccg acgagctacc
      121 aggctgctcc accccgcctc cgtggatgcg cactatactc cgctcgcgtt ttcatgcaac
      181 ctttttttta ccccgatcac ggatctggca tgattttgaa caaaaacaaa tcactaaagc
      241 ctattttttg tgcaaaattt gcgtcaggga gaggctgctc atttcattaa acgcggcggt
      301 ttgttatctt cgttgcgcct tattttttaa cctgaagaag agaacaatga aaggacgttg
      361 ggtaaagtac cttcttatgg gcacggttgt ggcaatgctt gccgcctgct cttccaaacc
      421 aaccgatcgc ggacagcaat ataaagacgg gaaatttacc cagcctttct ctctggtgaa
      481 ccagccagat gccgttggcg cgccgattaa cgccggtgat tttgccgagc aaattaacca
      541 tatccgtaat tcgtcaccgc gtctgtatgg caaccagagt aatgtttata acgcggtgca
      601 agagtggctg cgcgcaggcg gtgatacccg caatatgcgc cagttcggca ttgatgcctg
      661 gcagatggaa ggtgccgaca actatggtaa cgtgcagttt acaggctatt acacgccggt
      721 aattcaggcg cgccataccc gccagggcga gttccagtat cctatttacc gtatgccgcc
      781 aaaacgtggt cgtctgccgt ctcgtgcgga gatctacgcg ggggcattga gtgataaata
      841 tattctcgct tacagtaact ccctgatgga taacttcatt atggatgtgc agggtagtgg
      901 gtatatcgac tttggtgatg gcagtccgct taactttttc agctatgcag ggaaaaacgg
      961 tcatgcctat cgcagcattg gtaaggtgct gatcgaccgt ggcgaagtga aaaaagaaga
     1021 tatgtcgatg caggcgattc gtcactgggg cgaaacacac agtgaagccg aggttcgcga
     1081 gctgctggaa cagaacccgt ctttcgtctt ctttaaaccg caatcttttg caccggtgaa
     1141 aggggcaagt gcggtgccgc tggttggtcg cgcgtcagtt gcctctgatc gttccattat
     1201 tccgccaggt actaccttgc tggcagaagt gccgttgctg gataataacg gcaaatttaa
     1261 tggtcagtac gaactgcgtc tgatggtggc gctggatgtc ggtggtgcaa tcaaaggcca
     1321 acacttcgat atctatcaag ggatcgggcc ggaagccgga caccgcgcag gttggtacaa
     1381 ccactatgga cgtgtctggg tgctgaaaac cgccccgggc gcaggtaacg tctttagcgg
     1441 ctgatgtgtt attctgtata ccgtttttac ggatacgaca gggtgaggga taacctcacc
     1501 ctttttaatt ctgaggtgct atgtctgtgg taattagtga tgcatggcgt cagcgttttg
     1561 gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact gtttgctgac gcgcatattt
     1621 gtgtggttgg tatcggtggt gtcggttcct gggcggcgga agcgctggcg cgcacgggga
     1681 ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt caccaatacc aatcggcaaa
     1741 ttcacgctct gcgcgataac gtcgggctgg caaaagcgga agtcatggcg gagcgtattc
     1801 gccagattaa cccggagtgc cgtgtaacgg tggtggatga tttcgtgacg ccggataacg
     1861 tagcgcagta tatgagcgtg ggttattcgt acgtgattga tgccattgat agtgtacggc
     1921 ccaaagcggc gctgattgct tattgtcggc gcaataaaat cccgctggtc acgaccggtg
     1981 gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac cgatctggcg aaaacgattc
     2041 aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag cgattttggc gtagtgaaaa
     2101 acagtaaagg taagctcggc gtcgattgcg tgttttctac tgaagcgctg gtgtacccgc
     2161 agtcagacgg tactgtgtgt gcgatgaaag ccacggcaga agggccgaag cggatggatt
     2221 gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac ctttgggttt gttgcggttt
     2281 ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca gggttaaaca atcaggtcgg
     2341 atgagacgcg aacgtcttat ccgacccggt tctatgctta aacctgcttc gtcgcagcga
     2401 taatcgcctc gcttaacgca tttaacccct ggctgcgtga ggcgctaagt tgcgcacgca
     2461 atcccagctc atcaaacaat gccagcggcg actgtgcctg caattcggcg gcggttttcc
     2521 cctcaacggc agtcaacagc accgccagca ggccgcgcac aatgcgccct tcgctatcgc
     2581 caaagaagtg cattttaccg ttttcagtca cggtatatcc cagccagacg cggttttcgc
     2641 atccggcaat ctctttagcc tgcgctttta actcgtctgg caaagccgga agctgtttcc
     2701 ccagcatgat caactggcga tatttatctt cccattgcga caacggtgcg aaggtattgc
     2761 gtaacgtttc tgcggttacg gttgtgccga acggatgtcc ggcgaattgc gggttcgtca
     2821 ttaatccacc aataattcca gcgcgcggtc aacggcattc accagcgcat ccacatcact
     2881 ctttgtatta tatggcgcaa aagaggcgcg cagtgtgccg gttacgccta attctgccag
     2941 tagcggctga gcgcaatgtt gcccggcccg cagggcaata ccgtactccg ccagcagcgt
     3001 caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc agcaggctgg aatcctggca
     3061 gcggaatgag cggaagccag gacgttttgc cagcgcatct tccgccagcg tcgccaggct
     3121 gcgactccaa ctttctgcct ggttgatatc gtaatctgcc agccattcca gcgccgcgct
     3181 taatcctatg acaccagcca catttggcgt tcctgcttcc agtttccacg gcgcagattg
     3241 agtcgtgaag ccgtcaaaac tcacttcgtg aatcattttg ccgccaccca gccagggcga
     3301 catcgcttcc agcagttctg atttgccata cagcacgccg atacccgtcg ggccatacag
     3361 tttgtgacca gagaaagcat agaaatcgat atctagttgc tgaacatctg cggggaaatg
     3421 caccgccccc tgagcaccat caaccatcac caccatcccg gctgaatgag caaaggtaat
     3481 cgctcgcgcc agatccgggc aaccgccagt aacgttcgac atctgaccca acgccagaat
     3541 ccgactacgg ggagtaatca gttctggcaa caaatcgaca tccggcagtc gctgcgcatt
     3601 aagcggcaat ttcaccactt tggctccagt ttgttgggcg accatcagcc aggggacgag
     3661 gttagcgtgg tgttctgcca cgctgacaat aatctcatcg cccggttgca gacgcggacg
     3721 cgcatagcat tgtgccacca tgttgatgga ttcagtggtg ccgcgcgtcc agacgatagt
     3781 tttatcatcc ggtgcattca gtaattgcgc cactttctct cgtgcagctt cataacgcgc
     3841 ggtcaggcgt tgggcttcgg caaactggct gcgatggacg tttccggcgc tcaggctgta
     3901 aaactgtcga gtggcttcaa ccacggcttc aggtttaagc gcggtcgcgg cgctatcgag
     3961 atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga aactgcgcgg gattaaaaac
     4021 gttcatggta ctcctcggct tgatgcaccg atcgtgacgc aaaattcact ttgatacaag
     4081 gagagtgcta accatcagaa ttatctggat atcctggcga agccgcgtaa gtaacatatg
     4141 ctgaatagtg ttaggcgtta attttagcct gttaatcaat actattaaga tttattaatc
     4201 gctttaagga gaagatgatg aaaaagactg ccgcaattat ttctgcctgt atgctgactt
     4261 ttgccctgag cgcctgttcc ggttcgaact atgtgatgca caccaatgac ggacgtacca
     4321 tcgtctctga cggcaaacca cagactgata acgataccgg tatgatttcg tataaagacg
     4381 ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa agagatggtc gaactggatc
     4441 agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct acattaatca
     4501 ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc taccatggtc
     4561 tgaatcgcag accaattgca aacacaacaa cacaacatca caaccgtaag ccaaaagttc
     4621 accagaacac gcattccgat aaaacttttc gttccggctc aggaagtgcc gccactatag
     4681 gtatttgctg gtagaagctc aacggacaat ttataatggc tcagattaaa aaaactaata
     4741 ggttacacag tgtgatctaa ttgttaaatt aatttaacat caaagtttaa aagccatgtc
     4801 taaacgatta ccaccgctaa atgccttacg agtttttgat gccgcagcac gccatttaag
     4861 tttcactcgc gcagcagaag agcttttcgt gacccaggcc gcagtaagtc atcaaatcaa
     4921 gtctcttgag gattttttgg ggctaaaact gttccgccgc cgtaatcgtt cactcctgct
     4981 gaccgaggaa gggcaaagct atttcctcga tatcaaagag atattttcgc aattaaccga
     5041 agcgacgcgt aaactccagg cccgtagcgc caagggggcg ttgacggtca gtttactccc
     5101 cagtttcgcc attcattggt tggttccgcg actttccagc tttaattcag cttatccggg
     5161 aattgacgtt cgaatccagg cggttgatcg tcaggaagat aagctggcgg atgatgttga
     5221 tgtggcgata ttttatggtc ggggcaactg gccggggcta cgggtggaaa aactgtacgc
     5281 cgaatattta ttgccggtgt gttcgccgct actgctgaca ggcgaaaaac ccttgaagac
     5341 cccggaagat ctggctaaac atacgttatt acatgatgcg tcacgccgtg actggcagac
     5401 atatacccga cagttggggt taaatcatat caacgttcag caagggccaa tttttagtca
     5461 tagcgccatg gtgctgcaag cggctattca cgggcaggga gtggcgctgg caaataacgt
     5521 gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc ccgtttaatg atgttctggt
     5581 cagtaaaaac gctttttatc tggtttgtca tgacagccag gcagaactgg gtaaaatagc
     5641 cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa caagaaaaat tccgctttcg
     5701 ttatgaacaa taatttacgt agggtacgac catgaccagc cgttttatgc tgattttcgc
     5761 cgccattagc ggcttcattt ttgtggctct gggcgctttt ggcgcgcatg tgttaagtaa
     5821 aaccatgggg gccgttgaga tgggctggat ccagaccggc ctcgaatacc aggcgtttca
     5881 tacgctggcg atcttaggtc tggcggtggc aatgcagcgt cgcatcagta tctggtttta
     5941 ctggagtagc gttttcctcg cgttaggcac ggtgttgttc agcggcagcc tttattgcct
     6001 ggcgctgtcc catctgcgtt tgtgggcatt tgtcacaccg gttggcggcg tgagcttcct
     6061 cgcgggctgg gcgttaatgt tagttggtgc tatccgttta aagcgcaagg gcgtaagtca
     6121 tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc gcagaaatta
     6181 ccgataaagc cggccagcgg gaaattttcg gttttgcccg cgtgaaagag aatgcgggtt
     6241 atgtcattta tgaatgttat caacctgatg atggcgataa gttaatccgt gagctgccgt
     6301 tcagttcatt aatttttgcc cgccagtggt ttgtggtggg ggaactcttg cagcatttgc
     6361 cgccagaaga tcgtattacc cccattgtcg acatgttgca gggcgtagta gagaagggcg
     6421 gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa agagttgctg aaattctgcc
     6481 gtaaattcac cgtgccgcta cgcgctgcct tgcgcgatgc cggggtgctg gcgaactatg
     6541 aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc accaggctgc tgctataccg
     6601 gttactcata cagcaacaat aattcgccgt tctatatggg cattccgcgc ctgaaatttc
     6661 cggcagaggc gccgagtcgt tccacgctca aactggaaga ggcatttcat gtgtttattc
     6721 ctgcggatga gtgggatgaa cgcctggcga acgggatgtg ggcggtggat ttaggcgctt
     6781 gccctggcgg ctggacctac caactggtga agcgcaacat gtgggtttat tccgtcgaca
     6841 acggcccgat ggcgcaaagt ctgatggata ccgggcaggt gacgtggctg cgggaagacg
     6901 gtttcaaatt ccgtccgaca cgcagcaata tctcctggat ggtatgcgat atggttgaaa
     6961 aaccggctaa agttgcggca ttgatggcgc agtggctggt taatggctgg tgccgtgaaa
     7021 ccattttcaa tctcaaactg ccgatgaaaa aacgctacga agaagtatca cacaatctgg
     7081 cgtatattca ggcacagctt gatgaacatg gcataaatgc tcagattcag gcgcggcagt
     7141 tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg catctgggct gcggtgggtg
     7201 gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg tcttcggcct gctacccttt
     7261 ccgtcaggct gttaccaaag aagttgcaac ctggcgctca tcagaaataa tgtgcgttac
     7321 ctcatccaga tgcccgatgc gggcttcccc tgaacgatta aatttacttt tatcaatcaa
     7381 taacaacgat tgcgcggcac gttttaatag catcgatttg taatcagcgt tgatcgcatt
     7441 ggagtcccac agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa acagatcgat
     7501 ttccagcgat ttaagttggg aaatcagcga gggattgacg taacagccat atttgcgctc
     7561 aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt catggcaaat
     7621 cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc aactggcgtg ccagatacca
     7681 gcaagttgaa ctggcgtcta aggctatcac catcccttct tcaatccacg cgagcgcctc
     7741 gcgcgcgata tctgctttat gcgcataatg gcttttcagc ctgatgtgaa agggatcgcc
     7801 actgtcttga ttttgacggt ggatatattt agcgcgtcca tgattgcgca gaattttacc
     7861 ctgcgtctgt aattcattga gatcgcgacg aatggtttct ttactgacct ttagctgttc
     7921 agagagagct tccgtggtca ggctggtatg gttcagcagc aggtcgacta tcgcttgctg
     7981 gcgtgccgct ttcatatttt tcaccccatc attcgataac cgcccgcgtc atgcgggcgt
     8041 accgatatga gattacggtg ttaccccttt ttttaaaaga atattcccgt acttcgctcg
     8101 ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg caaaacgatt
     8161 gatgcggatg atgtcaggac acggggcttg tagtgaaagc gcattacggt aacgtcgttc
     8221 tacttcagga tcgagagtgt caccttctac cgctgccatc atcaccagcg gcggggcata
     8281 actgtccagc tcaaataatg ggataatcgc ttggagcaag tcgctcacca acaggccatc
     8341 agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg aaaaaataat
     8401 ttcatctcca tgtcccatct ctgccagcac tttcaatagt tcgggagaaa ttaacggcga
     8461 aattgttttc agcatttcac acttcctcta taaattcagg ttcagtttgc gggtagaaat
     8521 aacggtactg ataatgaatc tgtgcacggg cttcttccgg gctgttaaat tcccctacgc
     8581 cataccaacc gaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg agaactttta
     8641 ccggaatatc aagcatattg gctttaatct gattccacaa tgtgttgcga cttcctccac
     8701 cgactaacaa taattcagag gctttaaagt gcccgatttt ttccagcatc tgtagattgc
     8761 gctgtaattg cgcagttaac ccttccagcg ccgcgcgata gaaatgcccc cgcgtggtat
     8821 taagcgtcac tccttgccag ccagcgttct gacacgacaa taaatcacac tgcattttca
     8881 caccatccgc gccaggtgcg atcagacgag cttcttcaat caacatttgc cagggtgttt
     8941 cagccgtcca gaacagtttt ctcacccatt ccagcacgcc ggatgccagc cattgcatac
     9001 ctgggttata caaccctgcc tggctatcca gttcgcaggt agaatcggcg tactgactta
     9061 acagcgaagt atcaacctgg gcgctacgga ccattaaaat ttcccatgta ccggaagaga
     9121 gcacgggttc attttggtcg gcaccagcgc caaaaagggc gaactgggta tcgtgacctg
     9181 cggaaatcac cggtatgcca acgggtaagc cgagcattgc tgcggcgctg ttctgtagcg
     9241 taccaatctg ttcacccgct tccaccagac gagggaagag tcggcgtgga ataccggtgg
     9301 cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc cagcatctgg ctggttccgg
     9361 ccatcgtgat atcagtagtg aattcgccgg ttaaacggtg gttaatcagc gacgaaataa
     9421 agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac cacaccaact
     9481 tatataacgt attgaaacta aaggctccga cgccagaaat agcctgcaac cgctgtgcgg
     9541 agatcaactg ttcaatattg tccataaccg ctgctgttcg cggacatttc cagctaatga
     9601 tcggatagag cagattgcct tgcttatcta ccagagcgcc atccacacca aaggtggtga
     9661 cggcgatacc gcggatgtgg cattcagtca gttcactatt gatttgccga cagcaatcag
     9721 caaagcgttg caaaatggcg tctaaagacc actggtgcca ggtgttgttt tccatcgcga
     9781 tatcgctggc attaggcgtt gaggcgcggg caacaatttt tccctgccga ttaaccgcga
     9841 tggccctgac attggtcgcg ccacagtcga gtaccaggat aacttcttgt ttcataatcg
     9901 ctcctgcaat atagccggat aacattgctt atccggctaa ccactcttgc ttgttcaggc
     9961 ccggtagccg gagcgaccgg gcatcacatc agggagtaat gtattaacgc ttgtacaacg
    10021 gaccgtagtt ctggcaagcg cggtaatcct ggccttcaat atccatgccg tgcgcagccc
    10081 aggcagaagg acgatacact ttggtctctt caacgttgtg catacatacc gggatacgca
    10141 gcatggaggc gagagtgata aagtctgcgc caacgtggcc gatggtcaga accccatggt
    10201 tagcgcccca gttcgccatt accgagtaca catccgtaaa cggcccttta ccggtgaggc
    10261 gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg tttgttgagg atgtcatgca
    10321 catccttcgg caattccacg ctccagcctt ccgcgatttg cagtaccgat cccaggcctt
    10381 tgatgatgtt gacacgagtc atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag
    10441 agtaaccgcc gccacggaag tattcgtgga tcgccgggca ccattcggta gcggcgaggc
    10501 aagcgtcagc ctcttgctga gagatttccc agtgtggctt catcgtcggg ttaccttcgc
    10561 tgtcgcgttg tttacaggaa ccgtccagcg cagcagaacc tgagttgatc aaatggatga
    10621 tgccgtgttc tgccagtcca tcgagtttat gccccgttac acgctcaatt gcttctggtg
    10681 accagtaggt acgcacatcg gcaaatacct gagcggtgcc ggtgagctgg tgacccatta
    10741 gcattgccac gccgttaaga ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc
    10801 cattccagtc aaatgaactg ttgaggatcg cttcggcggt gtcaccattg ggatattgat
    10861 cggtccagtg acgttgcccc tggaagcccg cagcgatggc gttgtagcca agtgattctt
    10921 ccacgcgacc aatatcggcc agtttgctgt tgccttgcat catgtcgcgg atacacatcg
    10981 ccatcagtaa actttcgcgc agaactgcgc ggctttgctc ggcattgcgt tgatactgtt
    11041 tgttattttc atcttcgcca tagcggaagt ttttatcagc ccaggtcagt gccatttcca
    11101 gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc atatccaccg
    11161 cctggacttt cattcccagc caggattcaa agaagttgtg atcaacaata gaaccggcga
    11221 tacccatcga aacgccgccc agcgacagat agcttttacc tttcatgctg gcgacggcca
    11281 aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc gatgtgtcat
    11341 cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag
    11401 ctgccagagc cgctgccagg taaacagcgc cgggacgttc agtgccgtta aagccccaaa
    11461 tggctttcgg gcgggttgga tccatgtcga tggtttcact gccatagcac cagcaaggcg
    11521 ttaccgtaat ggtgaggcct acattctagc tgctgaattt ttcttcgcaa gcagcggctt
    11581 cagccatacc cgcgatacag gtatcggaaa tgacacactc gacggcagct ccgcaggcat
    11641 ggcgcagttt ctcggtcagc agtgcggccg tagctttcgc catattcatt gtttgttctt
    11701 caagcgactc acgaacaccc atgcgacgac cgtcaataac cgggcggata ccaattttcg
    11761 gtaagctgat ttttttcatt acagattcct cactttattc ggaaaatatg ttcagttagt
    11821 tgccgtttga gagcggaaac gggcaaagat aaagatgacc gcgaagcaga gtgtggggat
    11881 cagttcagca gtggggatgt tgcccgccgc gtcactgaca aaacccatga ccggagtgac
    11941 aataccgccg ccaataatgg tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc
    12001 gagattctta atgcccagcg agaagattgt tgggtactga atcgacataa aggcgctgca
    12061 taaagtcagg gctattaagc ccacatgacc gccagcgaag gctgagatca ggcacagtgc
    12121 catagcgatt aatgcgtagg cggccaggac tttgtgtggt gcgaagcgac tgatgagcca
    12181 ggtaccggtg aaacgaccaa taaagaagca caccatggtt ccggttaaat agttagcggc
    12241 aaagcctgca gtcatacctg gaatttcttc tacagcgtag cgaatcaaat agctccagca
    12301 ggccgtttgt gcgccaacgt agcagaattg cgctaatact gcccagcgcc agtggcgaat
    12361 acgcgctagg cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac tgtgattatc
    12421 actctgcaat gccgggaatt tcgtcagcat gatcagcagg gcgaccagta acacgatagc
    12481 cacgatgatc atataaggtg tctgtaccga taataccagg ctgtgtttat acgcactcaa
    12541 ttgctctgga gacattttat cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat
    12601 aagtctttgc ccaaagacaa ccgcgataat tgcgccaaac gagttaaatg tttgcgcaag
    12661 atttaagcgg aagtgaccac tgctttccgg ccctaatacc gtaacaaaag ggtttgcggc
    12721 agtttccaga caacctaatc cggctgcaat aataaatagg ccaactaaaa ataaggtgta
    12781 gttcattatt tctgcagcgg gccagaataa tgcagcgccc agggcatata aaaataaccc
    12841 agtaataatt cctgctttat aactgagttt tttcatcaat atcccagcag gaattgggat
    12901 aatgaaataa ccaaagtaaa aggccgattg gatcaggcca gcctggaaat ttgtcagcgt
    12961 aaaagcctgc tggaattgag gtaataaaat gtcgttaagg ttattggcta ccgcccaaag
    13021 aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa cttctgcttt gccctgcatc
    13081 tttatctacc gcacggtaac tctgcgtttg tattgatgtg tttcccatag catcctctta
    13141 ggttcagaag cttaagcacg taatcggtta tgttttcaaa agtgcaaaac cagcgagttc
    13201 gggcttcata tcagtgaata tttgcctgtc tgaaatgaac tcatggattc attttcaaat
    13261 aaaaaacgct ctgtaatatg acggcggtca cacttattag ctggaaggtg actttatgtg
    13321 actaccatca ctttaataat cgaaatttca tgcccgtttc agaaaataaa tcataaaaaa
    13381 cacaaaccag atgactgtat taattgttta tgccgaaaac gggcattcct ggcttgggtg
    13441 aatagaaaaa tgaccgtttt gataaatatt tttaagagcg gtcattttta atggggttat
    13501 aaaaagagaa cttgcagtta tttactgtga ttagttgagc taggtcacaa aatataagct
    13561 tgtgaatttc tttcttgtta atcaggaaga agatggtggg taattaaacg gctaattcaa
    13621 tagtgtgaaa ggaacaacat tattgccctg ttttgaatca gagagaggta gctaatggaa
    13681 cgaaataaac ttgctcgtca gattattgac acttgcctgg aaatgacccg cctgggactg
    13741 aaccagggga cagcggggaa cgtcagtgta cgttatcagg atgggatgct gattacgcct
    13801 acaggcattc catatgaaaa actgacggag tcgcatattg tctttattga tggcaacggt
    13861 aaacatgagg aaggaaagct cccctcaagc gaatggcgtt tccatatggc ggcctatcaa
    13921 agcagaccgg atgccaacgc ggttgttcac aatcatgccg ttcattgcac agcagtttcc
    13981 attcttaacc gaccgatccc cgctattcac tacatgattg cggcggcagg tggtaattct
    14041 attccttgcg cgccttatgc gacctttgga acacgcgaac tttctgaaca tgttgcgctg
    14101 gctctcaaaa atcgtaaggc aactttgtta caacatcatg ggcttatcgc ttgtgaggtg
    14161 aatctggaaa aagcgttatg gctggcgcat gaagttgaag tgctggcgca actttacgtg
    14221 gcgaccctgg cgattacgga cccggtgcca gtgctgagcg atgaagagat tgccgtagtg
    14281 ctggagaaat tcaaaaccta tgggttacgg attgaagagt aatttcgtaa agcaacaagg
    14341 agaaggatga tggctaacag aatgattctg aacgaaacgg catggtttgg tcggggtgct
    14401 gttggggctt taaccgatga ggtgaaacgc cgtggttatc agaaggcgct gatcgtcacc
    14461 gataaaacgc tggtgcaatg cggcgtggtg gcgaaagtga ccgataagat ggatgctgca
    14521 gggctggcat gggcgattta cgacggcgta gtgcccaacc caacaattac tgtcgtcaaa
    14581 gaagggctcg gtgtattcca gaatagcggc gcggattacc tgatcgctat tggtggtggt
    14641 tctccacagg atacttgtaa agcgattggc attatcagca acaatccgga gtttgccgat
    14701 gtgcgtagcc tggaagggct ttccccgacc aataaaccca gtgtaccgat tctggcaatt
    14761 cctaccacag caggtactgc ggcagaagtg accattaact acgtgatcac tgacgaagag
    14821 aaacggcgca agtttgtttg cgttgatccg catgatatcc cgcaggtggc gtttattgac
    14881 gctgacatga tggatggtat gcctccagcg ctgaaagctg cgacgggtgt cgatgcgctc
    14941 actcatgcta ttgaggggta tattacccgt ggcgcgtggg cgctaaccga tgcactgcac
    15001 attaaagcga ttgaaatcat tgctggggcg ctgcgaggat cggttgctgg tgataaggat
    15061 gccggagaag aaatggcgct cgggcagtat gttgcgggta tgggcttctc gaatgttggg
    15121 ttagggttgg tgcatggtat ggcgcatcca ctgggcgcgt tttacaacac tccacacggt
    15181 gttgcgaacg ccatcctgtt accgcatgtc atgcactata acgctgactt taccggtgag
    15241 aagtaccgcg atatcgcgcg cgttatgggc gtgaaagtgg aaggtatgag cctggaagag
    15301 gcgcgtaatg ccgctgttga agcggtgttt gctctcaacc gtgatgtcgg tattccgcca
    15361 catttgcgtg atgttggtgt acgcaaggaa gacattccgg cactggcgca ggcggcactg
    15421 gatgatgttt gtaccggtgg caacccgcgt gaagcaacgc ttgaggatat tgtagagctt
    15481 taccataccg cctggtaaat gcgctgatgt gataatgccg gatacgacgc tagccgcgtc
    15541 ttatccggta tataagtgca ttaaattcaa tgtgttgata atcgtttgca tccggctcgc
    15601 cgttaccgta ccaaccgcaa ttgctgtaaa ttgccgtcga tatgcaaatc ggtttgtaag
    15661 cgggcaatat cgcggcacag aaacgccatc tctttatggg tttctaattt tttgcgccac
    15721 ttttcggcaa ccgcatccag attctcatat atcccttcca gactctgaaa ctcgaccagc
    15781 agctgcgtgg cgctttttgg tccgattccc gcaacacccg gtacctttga actgctgatc
    15841 cccgcaagtc cccagtaatc gggcaactgc tgcggttgaa cgccaaattc tttatcgata
    15901 aatggcgcat ccagccaacg tttctggaag taatcacgaa tacgtaatgt cggtgaaagt
    15961 aactgacagt agcctttatc tgtcgaaaca atcgttgcct gatgcccggc ctgtgtcact
    16021 ttgaccgcca gcgtggcggc taagtcatcg gcttcgttgc cgctggttga ccagcacggg
    16081 acgccgcgtt gctcaaaggc ggcgcgtaat gcaggcatct cgtcgtgcaa ctcttccggc
    16141 attggcggtc gacccgcttt gtaatctggt aaacgctgat gacgccagcc gctactgcgg
    16201 ttttcatcat caaaaacggc gaccgcgtgg gttggctggc tgtgcataat gagctgatcg
    16261 agcgcatgct ggcaggtttc gacacagggc gatccctgaa cggcatgaat gcgacgaata
    16321 agattcagtg catcgacaat aagcaaatga acagccacga taaccccctt aaagatcagg
    16381 cgataagggt aacattcgat gccggaacag gctacggctg gatgagaaat cgggaagagg
    16441 cctcgcaaaa cgaggcctct ggagagcgat taatcgcagg caacgatctt cattgccagg
    16501 ccgccgcgag aggtttcgcg gtacttggcg ttcatatctt tacctgtttc gtacatggtt
    16561 tcgataactt tatcgaggca gacgcgcggc tcgctggtac ggcgtagcgc catacgtgcg
    16621 gcgttcaccg cttttactgc cgcaatggcg ttacgctcga tgcatggcac ctggacctgt
    16681 ccggcgaccg ggtcacacgt cagaccgagg ttgtgctcca tggcgatttc cgccgcgatg
    16741 cacacctgcg ccgggcttgc acctaacagt tctgccagac cagccgccgc cattgagcac
    16801 gccacaccaa cttcaccctg gcagccgact tcagcaccag aaattgacgc gttcatctta
    16861 taaagagaac caatggcact ggctaccagc aggtaacgag ccagtgagtt agcgttcact
    16921 tcgcggatga acttgtcgta gtacgccagc actgccggga taatcccgca cgcaccgtta
    16981 gtcggcgcag tcaccacgcg tccgccagca gcgttctctt cgttcactgc cagtgcaaac
    17041 atgttgatcc agtcaacaac cgccatcggg tcagtggtgg ttttatcctg gctgaccagc
    17101 atccggcgta gtgccgcagc acggcgtgga acgcgcagtt tgccaggcaa cacgccttcg
    17161 gtggaaatac cgcgctcaat accgccacgc atgacttccc atacgttcgc caggtgctgt
    17221 tccagctctt ctttgctgtg cagcgccagc tcatttttca tcatcaggcc agagagtgac
    17281 agcccggttt cctgacaatg tttttgcaga tcggctgctg aactgtacgg ataaggaact
    17341 tcaaccggtg cgctattctg ctggccaaaa tgctcttcat caacgataaa gccaccgcca
    17401 atagagtagt aagtctggct gtaaacgact ttatcgcccg ccagcgcggt aatgcgcata
    17461 ccgttttcat gcagagaaag gttgtctgca tggaagttca tgcactgatc aaccgggaac
    17521 tccacttcat gctgaccgtt tgccagcatc aggcgaccat gagtattcac atcctgaata
    17581 aaaccaggga tggaatcgat atccacggta tccggcaggt tacccgccag gcccataata
    17641 atggcgatat cagtgtggtg gcctttaccg gtcagagaga gcgagccgta cacgtcaacc
    17701 accacgcggg tcacgtcttt aagcagatta cgggcaatca gatcgtcggt aaattgttta
    17761 cccgctttca ttggtccaac ggtatgagaa ctggaagggc caatgccgat tttgaaaata
    17821 tcgaatacgc taatcatagg aaatacatcg tgttaaaacg gaggaagcgc cgcccgaaag
    17881 cggcgcgaaa ggacttagct gaacagagag tagaagattg cggagattgc aatcagaccc
    17941 atcacgacaa cgaatacgtt gctgatgtga ccgctgtact tacgcattgc cggtactttc
    18001 tgaattgcgt acatcggcat caggaacagg atcatcgcga tgattgggcc gcccagggtt
    18061 tcaatcatac ccaggatgct cgggttcagg gtggcaacaa tccaggtcgt taccagcatg
    18121 aacagcgcag tgatacggtt cagcttgttg atttcgatag acttaccttt accacgcaga
    18181 gatttaatca ccataccgtt gaagccttca cgtgcgccca ggtagtggcc gaggaaggat
    18241 ttggtgatag cgataatcgc gataatcgga gccatccacg cgataaccgg tgcgttaaag
    18301 tggttagcca ggtaagacag aatcgagatg ttctgctctt tagccgcagc caggtctgcc
    18361 ggagtcaggc tcagtacaca gctgaagacg aagaacatta cggtcagcac catcatgatg
    18421 tgtgcgaatg ccaggatctt cgagcatttc tgttctgcca tatcgccgta ctcttcacgc
    18481 ttcgcaacgg cgaaagaaga gatgatcgga gagtggttga aagagaacac cataaccgga
    18541 attgccagcc acagggtcat ccacagaccg tttccggttg cagatgcagt gtccagagac
    18601 agcgtttcca gtgctgcgcc gttccactgc gggatcaggt acagagccag cagcatcagt
    18661 acgccaacaa acgggaatac cagaatactc attgctttaa cgatcatctg ctcaccgaag
    18721 cgaacgatgg tcatcatacc cacgatcagg atcagcgaca gaatcgcacg cggcggtggt
    18781 gtcataccca gctggtgaga catgaagctt tcaactgtat tggtgattgc cacgctataa
    18841 accagcagga tcgggtagat agcgaagaag tagagcaggg taatcagttt acctgcgcca
    18901 ataccaaagt gttcttctac aacctcggtg atgtcttcgc ctgggttttt accagacagt
    18961 acgaagcgag tcaggccgcg gtgagcaaaa aatgtcatcg ggaacgcaag gatagccatg
    19021 atgatcagcg ggatcatacc gccaacaccg gcgttgattg gcaggaacag cacgcccgcg
    19081 ccgattgccg tgccgtaaag gcccagcatc cacatggtgt ctgtcttgcg ccaggcacta
    19141 cgagagtctt tcgacgcaat cgtgctggtt tgagtcgttt ccatctattt ctcctggagg
    19201 aatgcaaata attcagcttt tagccagttc agctcgggaa ggtgcctgac cgtaaaaatg
    19261 aaatctgtct atcggcgatg gcttaatcat ttaccgccgt aataatttga gggcggaaag
    19321 atacacacaa gttatgtatc tatcagtgat cttgatccca aatacaataa tctacttgga
    19381 agatagatta tcccaacgct ttactctgta aatcaaaagt aaaagagaat ttaagggcaa
    19441 tatttgccca ttaaacgaca tctgacttct tccggggcgc gcaatatatg taggttgaac
    19501 tgaaaaaatc aatactttta gttaatcatg gcgataattt ctttttcata gcgataaaat
    19561 gtggtttatg aaaataggga aacgtttgcg ttacaacata aattgtatga catattaact
    19621 gcaatgatat ttatcgcgtg agggtggtaa gaaacattca tataacaaat gaaataaatt
    19681 gatgggtatg tgaccaaaaa cgagaaaacc cggcaacaaa cgtcaccggg ggagggagat
    19741 tacgtgcaga tttcgtagca agggatgtag gctgagcctg gcaacttcat acgatgctgg
    19801 gcaacaaaac cctgtagcag gtcgtccata cgacgcataa tctctttatc gccgttgatt
    19861 ttgtaaggac caaactcttc aatggcgcga ataccgactt cttttacgtt acccgccaca
    19921 ataccggaga acgcacggcg cagatcggca gccagcactt caacaggttg atccgggtaa
    19981 agcttcagat tagccatatt ctcgtgagac ggctcaaacg gcatttgcaa atctggcgca
    20041 atgcgcattg accagttaaa gctgtaggca tcgcctgtgt cgcggcgatt ttctttcacc
    20101 agcggcatcg attttttcat ctgacgagcg acttcagcgg cgtcatcaat gatgatgcgg
    20161 taatggcggc gcgcgttttc acccaacgta tgcacgacaa actcgtccag tacgcggaag
    20221 tagtcggcgc tctctttcgg gccggtgagg aacaatggta aaacctgatc tttgttggcc
    20281 gggttcatta aaattcccag caaatacagc aactcttctg ccgtacccac accgccaggg
    20341 aagataatga tgccgtgagc gatacggaca aacgcttcca gacgtttttc gatatctggc
    20401 atgatgatca attcgttgac cagcgggtta ggcggttcag cggcgataat cgacggctct
    20461 gtcataccaa taaaacgact gtctttgtaa cgctgctgtg cgtgtccgac cgcagcacct
    20521 ttcatcggcg cttccatcgc tcccggacca cagccggtgc agatattcag ctcacgcagg
    20581 cccagctgat tcccgacgcg acgggcatac aaatactcgt tttcgttaat tgagtgtccg
    20641 ccccagcaga ccaccatatt tggcgcttca ccgacatgca gtgcgcgagc gttacgcaag
    20701 atggaaaaga ccaggttagt gatgtggacg gagttgtcga gattcaggtt gggaaaacga
    20761 acggtgttat ggatttgccc gtaaacgaag agaatgtcac gcagaaccgc gaacaagttg
    20821 gcctgcaacg cgcgaataat tcgcccatcg acaaaagcct cttccggggg attaatcagt
    20881 tccagcttta cgccgcgttc acggcgcagg acgttaatat cgaaattttc aaaacgagac
    20941 agcaattctt tgctgttatc ggtcaaacta ccggagttca gtacggcaag tgaacagttg
    21001 cgaaacagtt gatagaggtc gctgctggcg gtgcgtttaa gcatatccac ttccagctgc
    21061 gacaacatat ccatggagcc aagcgggcta atatgtgtaa tcaagaaaaa ctccttatgg
    21121 gacgaatatc gtcattccct ttgattacaa tagccctgcc tgatgtcctt tcacaacctt
    21181 ctgcgtgaat ccagcacgca aaattgagaa aaaatttatt gccgaaccag tcttgtggtc
    21241 gatgggacaa aatcgctatt actgcgccac gggttaatgt ccagaccgcc acgacgggta
    21301 taacgtgcgt aaacgctcaa tttttctggc tggcagaagc gtaacaggtc attaaagatg
    21361 cgttccacgc actgttcgtg gaactcgttg tgatgacgga atgagaccag gtaacgcagc
    21421 agtttttctc tgtcaatttg gcgtccacga tactgaattt ggatcgaacc ccaatctggt
    21481 tgatgggtga tcaggcagtt tgatttcagc aggtggctga caagcgtctc ttccactact
    21541 ttttcaccac aggtggcatt ctccagatag tcagtagtga attcatagtt atcgatagtg
    21601 atatcctggt catcaatgca agtgccatta aaatgaccta tcggctggcc ttccagttca
    21661 tcaagacgat ataacgccac gctaatctta ccctgagcgc aagtgcttaa gtcgcgctcc
    21721 agcgtctggc gcacctcatc ccagttatta aaacgcgtct ggttaaaact gttgagatag
    21781 agcttaaaac tcttcgactc aatcagattt acgctggtgt aatcaagttc aacatgaccg
    21841 acagcgacct gcggcaaacc tttcgcattc agccaggaaa gttcatacag cgtccagata
    21901 tccgtaccgt gaaaaggcag gttatccgct ttcagaccca gcgggtcgcg attcaggctg
    21961 cgtggaacgc cttgcagtag gctggcgtca taggtatccc ggtaatcggt tgattttcca
    22021 agagtcaggc ccgcaagtgc ctgatggttt gcataagaag acatgtttca ccgtcataaa
    22081 ccagataaac taaacgacaa gtgtatctgg tttaagtgaa gagagaaatt agtggacgat
    22141 ttgaccgcac aagccctgaa agattttact gcacgctact gcgatgcgtg gcatgaagag
    22201 cataaaagct ggccgttaag tgaggaactg tacggtgttc cttcaccgtg cattatttca
    22261 actaccgaag atgccgtata ctggcaaccc cagccgttta ccggggaaca aaatgtaaac
    22321 gcggttgaac gcgcttttga tattgtgata caacccacaa ttcatacctt ttatacgact
    22381 cagtttgccg gggatatgca cgcgcagttt ggcgatatca aactgacatt gctgcaaacc
    22441 tggagtgaag acgacttccg tcgggtgcag gaaaatctga ttggtcatct ggtgactcag
    22501 aaaaggctaa aactgccgcc cacgctattt atcgcgacgc tggaagaaga gttagaggtc
    22561 atctcggtgt gtaatctctc cggtgaagtg tgcaaagaga cgctgggaac acgtaaacgg
    22621 acgcatttag cctcaaatct tgcggaattc cttaatcaac ttaagcctct tctgtaatcc
    22681 aaatacccca tctggttgtg agagatctct tacagaccct gtaggagatc gccaggaaat
    22741 aagcgaatac ttaaaaagat aagaatcgtt attttcattt aaaatcaaaa tgttgattgt
    22801 taattcttaa ctttcatatg aaattttcct taaggcattg tctgtaagcg ccttgtaaga
    22861 cttcgcgaaa aagacgattc tatcttcgtc gacagggagt cagacaacga agtgaacatc
    22921 aggatgatga cacttctgca ggacacacca ggatggtgtt tcagggaaag gcttctggat
    22981 gaagcgaaga ggatgacgca ggacgcgtta aaggacacct ccaggatgga gaatgagaac
    23041 cggtcaggat gattcggtgg gtcaggaagg ccagggacac ttcaggatga agtatcacat
    23101 cggggtggtg tgagcaggaa gcaatagttc aggatgaacg attggccgca aggccagagg
    23161 aaaagttgtc aaggatgagc agggagcaac aaaagtagct ggaatgctgc gaaacgaacc
    23221 gggagcactg tgaatacagt gctccctttt tttattcctg ctatccttcg cggcagtttt
    23281 tcttattgaa gttgctttat gaccactcat gaccgcgttc gtctccagtt gcagacgctt
    23341 gaagcgttac tgcgtgaaca tcagcactgg cgaaacgatg aaccccagcc gcatcaattt
    23401 aatagtaccc aaccgttctt tatggacacc atggaaccac tggagtggtt gcagtgggtg
    23461 ctgatcccgc gaatgcacga tttgttaaat aacaaccagc cgttaccggg cgcttttgct
    23521 gttgcaccct attatgaaat ggcgctggcg acggaccatc cgcaacgcgc gcttattctg
    23581 gctgagttgg aaaaactgga tgctcttttt gcggatgatg cgagctaatg ctggaaatac
    23641 tctatcagga tgaatggctg gttgcggtaa ataaaccctc cggctggctg gttcaccgta
    23701 gctggctgga tcgcgacgag aaagtagtgg tcatgcaaac cgtgcgtgac cagataggac
    23761 agcatgtttt tactgctcat cgtctggacc gacccacttc tggtgtgttg ttgatgggat
    23821 tatccagcga ggccggacgg ctgctggcac aacagtttga acagcaccaa atccagaaac
    23881 gttaccatgc gattgtgcgc ggctggttga tggaagaagc ggtgctggat tatccactgg
    23941 tggaagaact ggacaaaatc gctgataaat ttgcccgcga agataaaggc ccgcagccag
    24001 cagtgacgca ttatcgcggt ctggcgaccg tagaaatgcc ggtagcgacc ggacgttacc
    24061 cgaccacgcg ctacggcctg gtggaactgg agccgaaaac cggacgcaaa catcagcttc
    24121 gccgacatct tgcccatttg cgtcatccga ttattggcga tagcaaacat ggcgatttac
    24181 gccagaatcg cagcggtgct gagcattttg gcctccagcg gttaatgcta catgccagtc
    24241 agttgtcatt gacgcatcct tttactggcg agccgctgac tattcacgcg ggcctggacg
    24301 acacctggat gcaggcgtta tcacaatttg gctggcgcgg tctgcttcct gaaaatgaaa
    24361 gggttgagtt cagtgcgcca tcgggtcagg atggagagat aagttcctaa tccagggaga
    24421 aaacggtatg gcggaaattg gtatttttgt cggcaccatg tacggaaatt cgctgttagt
    24481 ggcggaagaa gcagaagcaa ttctgaccgc acagggccac aaagcaacgg tatttgaaga
    24541 tcctgaatta agcgactggc tgccttatca ggataagtat gttctggtgg ttacgtccac
    24601 gaccgggcag ggcgaccttc ctgatagcat tgtgccgctc tttcagggaa tcaaagatag
    24661 tctgggcttc cagccgaatc tgcgttatgg cgtgattgcg ctcggcgaca gcagttatgt
    24721 aaatttctgc aatggcggca aacagttcga tgccttgttg caggaacaga gcgctcagcg
    24781 ggttggcgaa atgctgttga ttgacgccag tgagaacccg gaaccggaaa cggaatcaaa
    24841 tccgtgggtc gaacagtggg gcacgctgtt gtcctgatat gttcagcgag cggtaaatgt
    24901 cgttttagcg gtgctgaatc gaatcttttc aggcaaatgc cagtaaaaac tgcttcatag
    24961 cgcggatttt tactggcgtt tgcctggagt caagcgatcc atttcatact cttctttatt
    25021 tcttcgtttt aacccttcct ttcttgttct tgttttcatt tccgtgaagt ggattccacc
    25081 gtccagggct aatgccaaaa tcgggcctca ttgaacgcat taatgttgtg ttgttgcacg
    25141 gtgagccgct atggcgcgct ttttatactg ctattgccag atataaacac gcgccgtatt
    25201 cggcgaacga cctataaaaa cggcaaaaaa caccctacgt cacctctgat ttcctggcta
    25261 tgtcgcagtc cagagtgagc gtggctaacg cgaattttca ggagtgcaac aatgagttct
    25321 ttaagtcagg ctgcgagcag tgtggaaaaa cgcacgaatg ctcgttactg gatagtggtg
    25381 atgttgttta tcgtcacatc cttcaactac ggcgaccgcg ctacgctttc tattgccggt
    25441 tcggaaatgg ccaaagatat cggccttgat cccgtgggaa tgggctatgt gttctctgcc
    25501 ttctcatggg cttatgttat cgggcagatc cctggtggtt ggttgctgga tcgttttggt
    25561 tcaaaacgcg tctacttctg gtcgatcttt atctggtcga tgtttacctt gctgcaaggc
    25621 ttcgtcgata tctttagtgg attcggcatt atcgttgccc tgtttactct gcgcttcctg
    25681 gtcgggcttg ctgaagcgcc atctttcccc ggcaacagtc acattgttgc ggcctggttt
    25741 ccggcgcagg aaaggggaac ggcggtgtcg atttttaact ccgctcaata cttcgcaacg
    25801 gtgatcttcg cgccgattat gggctggctg acgcatgaag tgggctggtc acacgtcttc
    25861 ttctttatgg gcggtctggg gattgtcatc agctttatct ggttgaaagt catccacgag
    25921 ccaaatcaac atccgggggt aaatcagaaa gagctggagt acatcgccgc gggtggcgcg
    25981 ctgatcaata tggatcagca aaacaccaaa gttaaagtgc cgttcagcgt gaagtggggg
    26041 cagatcaaac aactgctcgg gtcacggatg atgatcggcg tttatatcgg tcagtactgt
    26101 atcaacgccc tgacttactt tttcattacc tggtttccgg tttatctggt gcaggcgcgt
    26161 gggatgtcga ttctgaaagc gggctttgtg gcttccgttc cggcggtttg cggttttatc
    26221 ggcggtgtgc tgggtgggat tatttccgac tggctgatgc gtcgcacggg atcgctgaac
    26281 attgcgcgta aaacaccgat cgtaatgggc atgttgctgt cgatggtgat ggtgttctgc
    26341 aactacgtca acgttgagtg gatgatcatc ggctttatgg cgctggcctt cttcggtaag
    26401 ggcatcgggg cgctgggttg ggcagtaatg gcagataccg cgccaaaaga gatcagcggt
    26461 ctttccggtg gcctgttcaa catgttcggt aacatttctg gcatcgtcac gccaatcgca
    26521 attggttata tcgttggcac gactggctcc tttaatgggg cgctgattta tgttggtgtt
    26581 catgccttaa tcgcggtact gagctacctg gtgctggtgg gcgatatcaa gcgtatcgag
    26641 ctgaaacctg tcgcggggca ataagatgac gacacaatcc agtcctgtta ttactgatat
    26701 gaaagtcatt ccggtcgccg ggcacgatag catgttgctt aatattggtg gggcacataa
    26761 cgcatatttc acccgcaata ttgtggtact caccgataac gccgggcata ccggcattgg
    26821 tgaagcgccg ggcggagagg tgatttatca aacactggtc gatgctattc cgatggttct
    26881 gggccaggaa gttgcgcgcc tgaataaagt ggttcagcag gtgcataaag gtaatcaggc
    26941 agccgatttt gataccttcg gcaaaggtgc ctggactttt gaattgcgcg ttaatgccgt
    27001 ggcggcgctg gaagccgcct tgcttgacct gctaggtaag gcgctgaatg ttccggtctg
    27061 cgaactgttg gggccaggca agcaacgcga gactattacc gtcctcggtt atctgtttta
    27121 tatcggtgat cggaccaaaa ccgatcttcc ttatctggaa aatacgccgg gcaaccatga
    27181 gtggtatcag ttgcgccatc agaaagcgat gaacagcgaa gccgttgtgc gtctggcgga
    27241 agcctcacag gatcgctatg gctttaaaga tttcaaactt aagggcgggg tgttacctgg
    27301 cgagcaagaa atcgacactg ttcgtgcatt gaagaaacgc ttccctgatg cgcggattac
    27361 cgttgatccc aacggtgcct ggctacttga tgaagccatt tctctatgca aagggctgaa
    27421 tgatgttctt acctatgccg aagatccatg cggcgcagaa cagggtttct ccggtcgtga
    27481 agttatggcg gagtttcggc gggcgaccgg cttgcccgtc gcgactaaca tgatcgccac
    27541 caactggcgc gaaatgggtc atgcggtgat gctcaatgcg gtagatattc cacttgccga
    27601 tccgcacttc tggacgcttt ccggtgcagt ccgtgtggcg cagctttgcg acgactgggg
    27661 gctgacctgg ggctgccatt ctaataacca tttcgatatc tctctggcga tgtttaccca
    27721 tgtgggcgcg gcggcaccgg gtaatcctac cgctatcgat acccactgga tttggcagga
    27781 gggcgattgt cgcctgacca aaaatccgct ggagattaaa aacggaaaaa ttgccgttcc
    27841 tgatgcgccc ggtctgggcg tggaactgga ctgggaacag gtacaaaagg cacatgaggc
    27901 ctataaacgt ctgcctggcg gtgcgcgtaa cgacgcaggt ccgatgcagt acctgatccc
    27961 cggctggacc tttgaccgta aacgtcccgt tttcggccgt cattgattct gaaaaaggac
    28021 ataaatatga gttctcaatt tacgacgtct gttgttactg aaatgcaggt catcccggtg
    28081 gcgggtcatg acagtatgct gatgaatctg agtggggcac acgcaccgtt ctttacgcgt
    28141 aatattgtga ttatcaaaga taattccggt cacactggcg taggggaaat tcccggcggc
    28201 gagaaaatcc gtaaaacgct ggaagatgcg attccgctgg tggtaggtaa aacgctgggt
    28261 gaatacaaaa acgttctgac gctggtgcgt aatacttttg ccgatcgtga tgctggtggg
    28321 cgcggtttgc agacatttga tttgcgtacc actattcatg tagttaccgg gatagaagcg
    28381 gcaatgctgg atctgctggg gcagcatctg ggggtaaacg tggcatcgct gctgggcgat
    28441 ggtcaacagc gtagcgaagt cgaaatgctc ggttatctgt tcttcgtcgg taatcgcaaa
    28501 gccacacctc tgccgtatca aagccagccg gatgactcat gcgactggta tcgcctgcgt
    28561 catgaagaag cgatgacgcc ggatgcggtg gtgcgcctag cggaagcggc gtatgaaaaa
    28621 tatggcttca acgatttcaa actgaaaggc ggtgtactgg ccggggaaga agaagccgag
    28681 tctattgtgg cactggcgca acgcttcccg caggcgcgta ttacgctcga tcctaacggt
    28741 gcctggtcgc tgaacgaagc aattaaaatt ggtaaatacc tgaaaggttc gctggcttat
    28801 gcagaagatc cgtatggtgc ggagcaaggt ttctccgggc gtgaagtgat ggcagagttc
    28861 cgtcgcgcga ctggcctgcc gactgcaacc aatatgatcg ccaccgactg gcggcagatg
    28921 ggccatacgc tctcgttaca atccgttgat atcccgctgg cggatccgca tttctggact
    28981 atgcaaggtt cggtacgtgt ggcgcaaatg tgccatgaat ttggcctgac ctggggttca
    29041 cactcaaaca accacttcga tatttccctg gcgatgttta cccatgttgc cgccgctgca
    29101 ccgggtaaaa tcaccgcgat tgatacgcac tggatttggc aggaaggcaa tcagcgcttg
    29161 accaaagaac cgtttgagat caaaggcggt ctggttcagg tgccggaaaa accggggctg
    29221 ggtgtagaaa tcgatatgga tcaagtgatg aaagcccatg agctgtatca gaaacacggg
    29281 cttggcgcgc gtgacgatgc gatgggaatg cagtatctga ttcctggctg gacgttcgat
    29341 aacaagcgcc cgtgcatggt gcgttaagac agaaaaccaa aaccggctct atctggagcc
    29401 ggcttgagac tgatgctaaa cgcaaaatag cctgttgtgc tatgtatcag gtctacatca
    29461 ttttttaatt cattgaattt aattggtttt gtaggccgga taaggcgtta acgccgcatc
    29521 cggcataaac acaggcactt tgtcaccaat ctgaaaccag cgtcataaaa agccgattgc
    29581 tactcgacaa gacgtccgtt acccgagaat tttgctggct tcgcgcgcga cattatccat
    29641 ctcgtccaac agttccagca gctccggctc caaatcttct tctttagtac cgctacgtag
    29701 ctgttgttcg atcagttggc agagattctt catacgcggc acaccgctat agccgcaact
    29761 gccatgcagt ttatgaatca aatcaaccag gccttccggg ttttctccaa ccagctgctc
    29821 ctcaactttg ttgcgaactt caggcaggaa atcgagtaac atttgcagca tatcgcgcgc
    29881 tagatcggtt tttcctgctg cctggcgtag tgccagttgc cagtcgaggg tcgcattcgg
    29941 gttcaccaca atttcgttga cttcgggcgt cacgacgcga gaggaaatac cgctgccagg
    30001 cttgtagcgc aacaacaaat tatgcaatcg ctcttcttca atcggtttcg ccagataatc
    30061 gctcatccct gcgccaagca gcttctcttt ttgcccggcc attgcatgcg ccgttaccgc
    30121 gataaccggc gtttgttgct gatgggggag ctggtggatg agctcgcagg cccgaatgcc
    30181 atccatgtca ggcatttgaa tatccattaa gatcaaatcg aacggcatct gtttcgcccg
    30241 ttcaaccgcc tgatgcccgc tatcgcaaag ttccacatgt tgcaccatat cttccagcaa
    30301 tgcgccgata agtttcaggt tagcggggtt gtcatcaact gccatgactg tcattgccag
    30361 cttgctttca tcggttacag gcaaaagcgt gttttgtttg tgatgacaaa attccgtcag
    30421 ggcaggcaac aggcgcgtag gtgttaatgg tttcagcaga cacgcgccga taccatcttg
    30481 cttgagtttt tctgcattga cttgtgcatg gcaaggaagt gccagcatca ggaaatcggt
    30541 catcgatacc gctttcgcta atcgctcatg ttgcattgtt agcggctcgc ggaaggtcac
    30601 cgcgatgcct aacaacatca tgtcgtaatg cgcgggaggc agcgcggaga acgttgggct
    30661 ataaaccact tccagcggcg tttcacttaa aatatccagc gtgcattgcg ctgctgcgga
    30721 gtttggttcg acataggcca ggcgcttacc ggcgaggcac tgggtggatg gcccttcgat
    30781 aataatgttc gggttcagat cgagattaat gtggaaccag aaagttgaac cgcgattcgg
    30841 ctggctatgg aacgaaatat cgccgcccat ttcattaacc agtttttgtg taatcaccag
    30901 ccccagaccg gtgccaccat gacggcggga aatactggca tcagcctgtc ggaaggcctg
    30961 gaataagcgc gattgatcgc gttcaggaat accaatgccg gtatcccgaa tctgcacttc
    31021 aatctgcact ttggtattac tcagcgcacg tttttctacc agaatatcaa tgttgccatt
    31081 ctcggtgaat ttaattgcat tccccaccag gttagtgatg atttgctgta atcgcagtgg
    31141 gtcgccgatc acgttatcag gcacgtcgct tttaatattg agcgtcagtt ccaacccttt
    31201 atcgtgagaa gaatgtgcca gcagagtaac gacttcatcc agcgtgctgc gtagtgggaa
    31261 tggaatactt tccagaatca gcttacctgc ttccagtttc gagaagtcga gaacatcatt
    31321 aataattgcc agcaaattat ttgccgaacg ttcaatcgta ttcaggtgat cgcgctgcgt
    31381 tggtgttaat tctgttttca gcgtcaggcg ggtaaagcca ataacaccat tcagtggtgt
    31441 acgcagctcg tgtgacatat ttgccagaaa ctcggattta atacgcgccg cttcctgggc
    31501 gcgctttttc gccagatcta actcaacgtt ctgaatttcc atctgctcca gcgtttcacg
    31561 cagatcggac gtcgcctggt cgatattgtg ctgcatctct tcgtgataag cagccagcga
    31621 cattgccatc gagttgatac cgtttttcag catatccagc tcgccgagca taaatccttc
    31681 cacccggctg tcgagttgcc cgcgacggat gcggtcgacg gtattcacca tgttgcgaat
    31741 cggaccggtt acatcgcgca ttaagcgcca gccaaaaata agcgcaatac cgatacaaaa
    31801 cagcatcatc acgctggaaa taaagatctc tttatattgc tgcaagcgaa ccgatttaag
    31861 atccagctcc agcgcaatat atcccaacat attttgacta tttttggcat cgctactggg
    31921 cgattcgtcg ggggagtaac tctcagaaat aatcggcgtg cggaggatca taatatcgcc
    31981 gtcacgagtg acagtgagct ggcgaggaaa cggcacgttg ctgccgagct gcattgatga
    32041 gggatcaaga tgaaaattgg aggtgacaaa gagtcggtta ttttcatcat aaaccgaaat
    32101 cgcgcgaaca atatcggaat ggcgacgatg cagtacgctt attaactgac cgatagattc
    32161 gcgattttgc aggctcatgc catattcagt agaaactgca agcggctcaa taatgctggc
    32221 tccggcatct tccagttgac gctgcaagtc gttatagcga tgcacaacga aaaagatact
    32281 cagcaataaa ccaataagga cggtcggtgc caggatcaga atcatcatgc gtgcgcgcag
    32341 gctgtagttg gtcatggagt tccgttatgg gacaattaag gtcacactgt taaattgaga
    32401 aagtctcgcc aatggcgcaa ttctactctg caaaacgacg cacgacgacg cgtcagatca
    32461 taaccgtttc agtcaacgac ctcgactctt ttggtcaggg cgtggcgcga cataacggca
    32521 aaacgctatt tatccccgga ttattgccgc aggaaaacgc ggaagttact gttactgaag
    32581 ataaaaaaca gtatgcccgc gctaaagtcg tacgccggtt aagcgatagc ccgggacgcg
    32641 aaacgccacg ctgtcctcat tttggcgtat gcggcggctg tcagcaacaa cacgccagcg
    32701 tggatttaca gcagcgaagc aaaagtgcgg cactcgcccg attaatgaaa cacgaagtct
    32761 ctgaagtgat cgccgatgtt ccctggggct atcgccgtcg cgcgcgttta agtctgaact
    32821 acttaccgaa aacacagcaa cttcagatgg ggtttcgcaa agcgggctcc agtgacattg
    32881 tcgacgttaa acaatgcccc attttagtgc cccaacttga agcattgctg cccaaagtca
    32941 tggcatgctt gggcagctta caagttatgc gccatcttgg tcatgttgaa ctggtacagg
    33001 caaccagcgg cacgctgatg attttgcgcc ataccgcacc gctaagttcg gcagatcgcg
    33061 aaaaactgga acgcttttcg cattctgaag gcctggatct gtatctcgcc cccgatagtg
    33121 agatactcga aaccgtctct ggtgagatgc cctggtatga ctcaaatggg ttgtgcttaa
    33181 cttttagccc gcgcgatttt attcaggtca atgcgggtgt gaaccaaaaa atggtagcgc
    33241 gtgcgttgga atggctggat gtgcaacctg aagatcgcgt actggatttg ttctgcggta
    33301 tgggcaactt tacactgcca ttggcgacac aagctgccag tgtggtgggt gtagaaggcg
    33361 ttccggcgct ggtggaaaaa ggccagcaga atgcgcgtct taatggctta cagaatgtga
    33421 cgttttatca cgaaaatctt gaagaagatg tcacaaagca gccgtgggcg aaaaacggct
    33481 tcgataaagt gttgctggac ccggcgcgag caggtgccgc aggtgttatg cagcaaatta
    33541 taaaactgga acctattcgt atagtttatg tatcctgtaa ccctgcaacg ctggctcggg
    33601 atagcgaagc gttattaaaa gcaggatata ccattgcgcg actggcgatg ctggatatgt
    33661 tcccacacac gggacatctg gaatcgatgg tacttttctc gcgcgttaaa tagttgcgat
    33721 ttgccgattt cggcaggtct ggtccctaaa ggagaggacg atggttgcgg taagaagtgc
    33781 acatatcaat aaggctggtg aatttgatcc ggaaaaatgg atcgcaagtc tgggtattac
    33841 cagccagaag tcgtgtgagt gcttagccga aacttgggcg tattgtctgc aacagacgca
    33901 ggggcatccg gatgccagtc tgttattgtg gcgtggtgtt gagatggtgg agatcctctc
    33961 gacattaagt atggacattg acacgctgcg ggcggcgctg ctgttccctc tggctgatgc
    34021 caacgtagtc agcgaagatg tgctgcgtga gagcgtcggt aagtcggttg ttaaccttat
    34081 tcacggcgtg cgtgatatgg cggcgatccg ccagctgaaa gcgacgcaca ctgattctgt
    34141 ttcctccgaa caggtcgata acgttcgccg gatgttattg gcgatggtcg atgattttcg
    34201 ctgcgtagtc atcaaactgg cggagcgtat tgctcatctg cgcgaagtaa aagatgcgcc
    34261 ggaagatgaa cgtgtactgg cggcaaaaga gtgtaccaac atctacgcac cgctggctaa
    34321 ccgtctcgga atcggacaac tgaaatggga actggaagat tactgcttcc gttacctcca
    34381 tccaaccgaa tacaaacgaa ttgccaaact gctgcatgaa cggcgtctcg accgcgaaca
    34441 ctacatcgaa gagttcgttg gtcatctgcg cgctgagatg aaagctgaag gcgttaaagc
    34501 ggaagtgtat ggtcgtccga aacacatcta cagcatctgg cgtaaaatgc agaaaaagaa
    34561 cctcgccttt gatgagctgt ttgatgtgcg tgcggtacgt attgtcgccg agcgtttaca
    34621 ggattgctat gccgcactgg ggatagtgca cactcactat cgccacctgc cggatgagtt
    34681 tgacgattat gtcgctaacc cgaaaccaaa cggttatcag tctattcata ccgtggttct
    34741 ggggccaggt ggcaaaaccg ttgagatcca aatccgcacc aaacagatgc atgaagatgc
    34801 agagttgggt gttgctgcgc actggaaata taaagagggc gcggcagctg gcggcgcacg
    34861 ttcgggacat gaagaccgga ttgcctggct gcgtaaactg attgcgtggc aggaagagat
    34921 ggctgattcc ggcgaaatgc tcgacgaagt acgcagccag gtctttgacg accgggtgta
    34981 tgtctttacg ccgaaaggtg atgtcgttga tttgcctgcg ggatcaacgc cgctggactt
    35041 cgcttaccac atccacagtg atgtcggaca ccgctgcatc ggggcaaaaa ttggcgggcg
    35101 cattgtgccg ttcacctacc agctgcagat gggcgaccag attgaaatta tcacccagaa
    35161 acagccgaac cccagccgtg actggttaaa cccaaacctc ggttacgtca caaccagccg
    35221 tgggcgttcg aaaattcacg cttggttccg taaacaggac cgtgacaaaa acattctggc
    35281 tgggcggcaa atccttgacg acgagctgga acatctgggg atcagcctga aagaagcaga
    35341 aaaacatctg ctgccgcgtt acaactttaa tgatgtcgac gagttgctgg cggcgattgg
    35401 tggcggggat atccgtctca atcagatggt gaacttcctg caatcgcaat ttaataagcc
    35461 gagtgccgaa gagcaggacg ccgccgcgct gaagcaactt cagcaaaaaa gctacacgcc
    35521 gcaaaaccgc agtaaagata acggtcgcgt ggtagtcgaa ggtgttggca acctgatgca
    35581 ccacatcgcg cgctgctgcc agccgattcc tggagatgag attgtcggct tcattaccca
    35641 ggggcgcggt atttcagtac accgcgccga ttgcgaacaa ctggcggaac tgcgctctca
    35701 tgcgccagaa cgcattgttg acgcggtatg gggtgagagc tactccgccg gatattcgct
    35761 ggtggtccgc gtggtggcta atgatcgtag tgggttgtta cgtgatatca cgaccattct
    35821 cgccaacgag aaggtgaacg tgcttggcgt tgccagccgt agcgacacaa aacagcaact
    35881 ggcaaccatc gacatgacca ttgagattta caacctgcag gtgctgggcc gcgtgctggg
    35941 taaactcaac caggtgccgg atgttatcga cgcgcgtcgg ttgcacggga gttaggcaga
    36001 aatttgctcg tatctacaat gtagattgat atatactgta tctacatatg atagcggttt
    36061 gaggaaaggg ttatgatcca cagtagcgta aagcgttggg gaaattcacc ggcggtgcgg
    36121 atcccggcta cgttaatgca ggcgctcaat ctgaatattg atgatgaagt gaagattgac
    36181 ctggtggatg gcaaattaat tattgagcca gtgcgtaaag agcccgtatt tacgcttgct
    36241 gaactggtca acgacatcac gccggaaaac ctccacgaga atatcgactg gggagagccg
    36301 aaagataagg aagtctggta atggtaagcc gatacgtacc cgatatgggc gatctgattt
    36361 gggttgattt tgacccgaca aaaggtagcg agcaagctgg acatcgtcca gctgttgtcc
    36421 tgagtccttt catgtacaac aacaaaacag gtatgtgtct gtgtgttcct tgtacaacgc
    36481 aatcaaaagg atatccgttc gaagttgttt tatccggtca ggaacgtgat ggcgtagcgt
    36541 tagctgatca ggtaaaaagt atcgcctggc gggcaagagg agcaacgaag aaaggaacag
    36601 ttgccccaga ggaattacaa ctcattaaag ccaaaattaa cgtactgatt gggtagtgtt
    36661 actaactcac ttctattctg gtcacaggtt tccacctgac agacccgcaa ttttcaggac
    36721 aattcaatga atcaaatcga ccgtttgctc actattatgc agcgcctgcg cgatccggaa
    36781 aacggctgcc cgtgggataa agagcagaca tttgccacca ttgcgcctta cacccttgaa
    36841 gaaacctacg aagtgctgga cgccatcgcc cgtgaagatt ttgacgatct tcgcggtgaa
    36901 ctgggcgatc tgctattcca ggtggtgttt tacgcgcaaa tggctcagga agaagggcgc
    36961 tttgacttta atgatatttg cgctgctatt agcgataaat tagagcgtcg ccatccgcat
    37021 gtttttgctg atagttctgc cgaaaacagt agtgaagtgc ttgcccgttg ggagcaaatc
    37081 aaaaccgaag agcgcgcgca gaaagcgcag cattcggcgc tggacgatat tcctcgtagt
    37141 ttaccggctt taatgcgggc gcaaaaaatc cagaaacgtt gcgccaacgt tggcttcgac
    37201 tggacgacgc ttggcccggt ggttgataaa gtctacgaag agatcgacga ggtgatgtac
    37261 gaggcgcggc aggctgttgt cgaccaggct aaactggagg aggaaatggg ggacctgctg
    37321 tttgccacgg ttaatctggc tcgccattta gggacgaaag cagaaatcgc attgcaaaaa
    37381 gcgaacgaaa aattcgagcg tcgttttcgc gaagtggagc gtattgttgc cgcgcgtgga
    37441 ctggaaatga caggtgttga cctcgaaaca atggaagaag tctggcaaca ggtaaaacgg
    37501 caggaaattg atctctaagg gaattacgcg gtcaagcgcc atttgtgtca ttttttaaat
    37561 gacaagcgct tgatttgcgt caaaaacatt taccccaaag gggctatttt ctcactcctg
    37621 atttcaatag tgcgctggcg aagaggaggg ataatgaaag tttgtggcac aggtcatgtt
    37681 cgggtatact gctttcccgt cctggttatt ccatcgtctt ttcaacctaa cttctcaggt
    37741 tcagcatgac aacgaactat atttttgtga ccggcggggt cgtatcctct ctgggtaaag
    37801 gcattgccgc agcctccctc gcagccattc ttgaagcccg tggcctcaat gtgaccatca
    37861 tgaaactgga tccgtacatc aacgtcgatc caggtactat gagcccaatc caacacgggg
    37921 aagtgttcgt tactgaagac ggcgctgaaa ccgacctgga cctggggcac tacgagcgtt
    37981 tcattcgtac caaaatgagc cgccgcaaca acttcaccac gggtcgtatc tactctgacg
    38041 ttctgcgtaa agaacgccgc ggtgactacc tcggcgcaac cgtgcaggtt attccgcaca
    38101 tcactaacgc aatcaaagag cgcgtgctgg aaggtggcga aggtcatgac gtagtactgg
    38161 tagaaatcgg cggtacagta ggtgatatcg aatccttgcc gttcctcgaa gcgattcgcc
    38221 agatggctgt tgaaattggc cgtgagcaca ctctgtttat gcacctgacg ctggtgccgt
    38281 acatggcagc gtctggtgaa gtcaaaacca aaccgactca gcactctgta aaagagctgc
    38341 tctccatcgg tatccagcct gacatcctga tttgtcgttc agatcgcgct gttccggcga
    38401 acgaacgtgc gaagattgca ttgttctgta atgttccgga aaaagcggtt atttctctga
    38461 aagacgtcga ttccatctat aaaattccgg gcctgttgaa atctcagggg ctggacgatt
    38521 atatttgtaa acgattcagc ttaaactgcc cggaagcaaa tctgtccgaa tgggaacagg
    38581 ttatcttcga agaagcgaat ccggtaagtg aagtcaccat cggtatggtc ggcaagtaca
    38641 ttgaactgcc ggatgcttat aaatcagtga tcgaagcact gaaacacggt gggctgaaga
    38701 atcgtgtcag cgtcaacatc aaactgatcg attcacaaga tgttgaaacg cgcggcgttg
    38761 aaatccttaa aggtctggac gcaatcctcg tacctggcgg tttcggctat cgtggcgtag
    38821 aaggcatgat tacgaccgcg cgttttgcgc gtgagaacaa tattccttat ctgggcattt
    38881 gcctgggtat gcaggtggcg ttaattgatt acgctcgcca tgttgccaac atggagaacg
    38941 ccaactctac ggaatttgtg ccagactgta agtacccggt tgtggcgctg attaccgagt
    39001 ggcgcgatga aaacggcaac gttgaagttc gtagcgagaa gagcgatctc ggcggtacca
    39061 tgcgtctcgg cgcacagcag tgccagttgg ttgacgatag cctggttcgc cagctgtaca
    39121 atgcgccgac aattgttgag cgtcatcgtc accgttacga agtcaacaac atgctgttga
    39181 aacagattga agatgcaggt ctgcgcgttg cgggccgttc cggggatgat cagttggtcg
    39241 agatcatcga agttccgaat cacccgtggt tcgtggcttg ccagttccat ccggagttta
    39301 cttctactcc acgtgatggt cacccgctgt ttgcaggctt tgtgaaagct gccagcgagt
    39361 tccagaaacg tcaggcgaag taagtaaaaa agttagaacg gcaacgcgta ccttgggtac
    39421 gcgttgtttg tctggagttt cagtttaact agtgacttga ggaaaaccta atgtccaaaa
    39481 tcgtaaaaat catcggtcgt gaaatcatcg actcccgtgg taacccgact gttgaagccg
    39541 aagtacatct ggagggtggt ttcgtcggta tggcagctgc tccgtcaggt gcttctactg
    39601 gttcccgtga agctctggaa ctgcgcgatg gcgacaaatc ccgtttcctg ggtaaaggcg
    39661 taaccaaagc tgttgctgcg gtaaacggcc cgatcgctca ggcactgatt ggcaaagacg
    39721 ctaaagatca ggctggcatt gacaagatca tgatcgacct ggacggcacc gaaaacaaat
    39781 ccaaattcgg cgcgaacgca atcctggctg tatctctggc taacgccaaa gctgctgctg
    39841 ccgataaagg gatgccgctg tacgagcaca tcgctgaact gaacggcact ccgggcaaat
    39901 actccatgcc ggttccgatg atgaacatca tcaacggtgg tgagcacgct gacaacaacg
    39961 ttgatatcca ggaattcatg attcagccgg ttggcgcgaa aactgtgaaa gaagccatcc
    40021 gcatgggttc tgaagttttc catcacctgg caaaagttct gaaagcgaaa ggcatgaaca
    40081 ctgcagttgg tgacgaaggt ggctatgcgc cgaacctggg ttccaacgct gaagctctgg
    40141 ctgttatcgc tgaagctgtt aaagctgctg gttatgaact gggcaaagac atcactctgg
    40201 cgatggactg cgcagcttct gaattctaca aagacggtaa atacgttctg gctggcgaag
    40261 gcaacaaagc tttcacctct gaagaattca ctcacttcct ggaagaactg accaaacagt
    40321 atccgatcgt atctatcgaa gacggtctgg acgaatctga ctgggacggt ttcgcatacc
    40381 agaccaaagt tctgggcgac aaaatccagc tggttggtga cgacctgttc gtaaccaaca
    40441 ccaagatcct gaaagaaggt atcgaaaaag gtatcgctaa ctccatcctg atcaaattca
    40501 accagatcgg ttctctgacc gaaactctgg ctgcaatcaa gatggcgaaa gatgctggct
    40561 acactgcagt tatctctcac cgttctggcg aaactgaaga cgctaccatc gctgacctgg
    40621 ctgttggtac tgctgcaggc cagatcaaaa ctggttctat gagccgttct gaccgtgttg
    40681 ctaaatacaa ccagctgatt cgtatcgaag aagctctggg cgaaaaagca ccgtacaacg
    40741 gtcgtaaaga gatcaaaggc caagcataag actgacttta tctgatttaa aaatgccagc
    40801 ctc
//
LOCUS       DAEEBB010000018        39785 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEEBB010000018 DAEEBB010000000
VERSION     DAEEBB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.018"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            4..435
                     /locus_tag="KLF07_000930"
     CDS             4..435
                     /locus_tag="KLF07_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBD9831016.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="KLF07_000931"
     CDS             518..1420
                     /locus_tag="KLF07_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBD9831017.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="KLF07_000932"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="KLF07_000932"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBD9831018.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="KLF07_000933"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="KLF07_000933"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBD9831019.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="KLF07_000934"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="KLF07_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBD9831020.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="KLF07_000935"
     CDS             complement(5330..6037)
                     /locus_tag="KLF07_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBD9831021.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="KLF07_000936"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="KLF07_000936"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBD9831022.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="KLF07_000937"
     CDS             complement(6895..7098)
                     /locus_tag="KLF07_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD9831023.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7589..7909
                     /gene="cyaY"
                     /locus_tag="KLF07_000938"
     CDS             7589..7909
                     /gene="cyaY"
                     /locus_tag="KLF07_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBD9831024.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7949..10495)
                     /gene="cyaA"
                     /locus_tag="KLF07_000939"
     CDS             complement(7949..10495)
                     /gene="cyaA"
                     /locus_tag="KLF07_000939"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBD9831025.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10882..11823
                     /gene="hemC"
                     /locus_tag="KLF07_000940"
     CDS             10882..11823
                     /gene="hemC"
                     /locus_tag="KLF07_000940"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBD9831026.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11820..12560
                     /gene="hemD"
                     /locus_tag="KLF07_000941"
     CDS             11820..12560
                     /gene="hemD"
                     /locus_tag="KLF07_000941"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBD9831027.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12582..13811
                     /gene="hemX"
                     /locus_tag="KLF07_000942"
     CDS             12582..13811
                     /gene="hemX"
                     /locus_tag="KLF07_000942"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD9831028.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            13814..15010
                     /gene="hemY"
                     /locus_tag="KLF07_000943"
     CDS             13814..15010
                     /gene="hemY"
                     /locus_tag="KLF07_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBD9831029.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15672..15833
                     /locus_tag="KLF07_000944"
     CDS             15672..15833
                     /locus_tag="KLF07_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831030.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15917..17152)
                     /locus_tag="KLF07_000945"
     CDS             complement(15917..17152)
                     /locus_tag="KLF07_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD9831031.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17299..17375)
                     /locus_tag="KLF07_000946"
     tRNA            complement(17299..17375)
                     /locus_tag="KLF07_000946"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17339..17341),aa:Pro,seq:tgg)
     gene            complement(17418..17504)
                     /locus_tag="KLF07_000947"
     tRNA            complement(17418..17504)
                     /locus_tag="KLF07_000947"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17468..17470),aa:Leu,seq:cag)
     gene            complement(17525..17600)
                     /locus_tag="KLF07_000948"
     tRNA            complement(17525..17600)
                     /locus_tag="KLF07_000948"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:His,seq:gtg)
     gene            complement(17659..17735)
                     /locus_tag="KLF07_000949"
     tRNA            complement(17659..17735)
                     /locus_tag="KLF07_000949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17699..17701),aa:Arg,seq:ccg)
     gene            complement(17836..19221)
                     /locus_tag="KLF07_000950"
     CDS             complement(17836..19221)
                     /locus_tag="KLF07_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD9831032.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19412..20152)
                     /gene="rffM"
                     /locus_tag="KLF07_000951"
     CDS             complement(19412..20152)
                     /gene="rffM"
                     /locus_tag="KLF07_000951"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBD9831033.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20155..21507)
                     /gene="wzyE"
                     /locus_tag="KLF07_000952"
     CDS             complement(20155..21507)
                     /gene="wzyE"
                     /locus_tag="KLF07_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBD9831034.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21504..22583)
                     /gene="rffT"
                     /locus_tag="KLF07_000953"
                     /gene_synonym="wecF"
     CDS             complement(21504..22583)
                     /gene="rffT"
                     /locus_tag="KLF07_000953"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBD9831035.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22580..23830)
                     /gene="wzxE"
                     /locus_tag="KLF07_000954"
     CDS             complement(22580..23830)
                     /gene="wzxE"
                     /locus_tag="KLF07_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBD9831036.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23832..24962)
                     /gene="rffA"
                     /locus_tag="KLF07_000955"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23832..24962)
                     /gene="rffA"
                     /locus_tag="KLF07_000955"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBD9831037.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24967..25641)
                     /gene="rffC"
                     /locus_tag="KLF07_000956"
                     /gene_synonym="wecD"
     CDS             complement(24967..25641)
                     /gene="rffC"
                     /locus_tag="KLF07_000956"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBD9831038.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25619..26500)
                     /gene="rfbA"
                     /locus_tag="KLF07_000957"
     CDS             complement(25619..26500)
                     /gene="rfbA"
                     /locus_tag="KLF07_000957"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD9831039.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26519..27586)
                     /gene="rffG"
                     /locus_tag="KLF07_000958"
     CDS             complement(26519..27586)
                     /gene="rffG"
                     /locus_tag="KLF07_000958"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD9831040.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27586..28848)
                     /gene="wecC"
                     /locus_tag="KLF07_000959"
     CDS             complement(27586..28848)
                     /gene="wecC"
                     /locus_tag="KLF07_000959"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBD9831041.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28845..29975)
                     /gene="wecB"
                     /locus_tag="KLF07_000960"
     CDS             complement(28845..29975)
                     /gene="wecB"
                     /locus_tag="KLF07_000960"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBD9831042.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30031..31077)
                     /gene="wzzE"
                     /locus_tag="KLF07_000961"
     CDS             complement(30031..31077)
                     /gene="wzzE"
                     /locus_tag="KLF07_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBD9831043.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31089..32192)
                     /gene="wecA"
                     /locus_tag="KLF07_000962"
     CDS             complement(31089..32192)
                     /gene="wecA"
                     /locus_tag="KLF07_000962"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBD9831044.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32432..33691)
                     /gene="rho"
                     /locus_tag="KLF07_000963"
     CDS             complement(32432..33691)
                     /gene="rho"
                     /locus_tag="KLF07_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBD9831045.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33776..33877)
                     /gene="rhoL"
                     /locus_tag="KLF07_000964"
     CDS             complement(33776..33877)
                     /gene="rhoL"
                     /locus_tag="KLF07_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBD9831046.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34018..34347)
                     /gene="trxA"
                     /locus_tag="KLF07_000965"
     CDS             complement(34018..34347)
                     /gene="trxA"
                     /locus_tag="KLF07_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBD9831047.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34478..35743
                     /gene="rhlB"
                     /locus_tag="KLF07_000966"
     CDS             34478..35743
                     /gene="rhlB"
                     /locus_tag="KLF07_000966"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBD9831048.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35877..37361
                     /gene="gppA"
                     /locus_tag="KLF07_000967"
     CDS             35877..37361
                     /gene="gppA"
                     /locus_tag="KLF07_000967"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBD9831049.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37408..39429)
                     /gene="rep"
                     /locus_tag="KLF07_000968"
     CDS             complement(37408..39429)
                     /gene="rep"
                     /locus_tag="KLF07_000968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBD9831050.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9869 a        10821 c        10042 g         9053 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgtt tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctgctt
     7141 tctgctttct gctttctgct ttctatcatc gcaggagaag acgtaaaagc aagcgtttag
     7201 cgactcttta tgaagtctga aaagtgagga aatgctgcga gtataacgct tactcgtggg
     7261 gatttatgtt ttgctggtag tttcgtgatc ggcacctcgc gccaggatgg ctctatgcac
     7321 tatcgcaatg tggtttgttt gttgtctttt ttagttccgt cagcgtgagg atgctcgctc
     7381 ggtaaacctg atcataatgt ttaccagaaa cagggaaagg cgtggtgtaa ctgcgcccta
     7441 atgaagagac aaatctttcg ctacacagag acatcattat tccggtgacg gatgaatcgt
     7501 gccggaacgc agccgcagag gtgcaaactt tgaagccctg accccatgcc gctatactgc
     7561 cgccatcact taaaaacagg atacaacaat gaacgacagt gaatttcatc gcctggctga
     7621 tcaactctgg ctgaccattg aagaacgcct ggacgactgg gatggcgaca gcgatatcga
     7681 ctgcgaaatc aacggcggcg tgctgaccat tacctttgaa aacggcagca aaatcattat
     7741 caaccgccag gaaccgctgc accaggtatg gctggcaacc aaacaaggcg gctaccattt
     7801 tgatctgaaa ggcgatgagt ggatttgcga tcgcagcggc gaaaccttct gggatttgct
     7861 ggaacaggcg gcgacgcagc aggcgggtga aacagtcagt ttccgctaag attgcatgcc
     7921 ggataagcct cgctttccgg cacgttcatc acgaaaaata ttgctgtaat agcggcgtat
     7981 cgtgatcctg attggcaggc ggcatgttac cgatagattt tgtgcggaac ggaatcacct
     8041 gttcacgacc atcaaccttc acaatctgat agaactgcgg caggttgaag ttgatgaagc
     8101 ttgagccgta ggtgaagcgg tcgtgcgatg acgagtagaa gcgactgacg tcacgcacca
     8161 gttcctcttt gctgccttcg cagtggtgat acacctcaac ccggttgctt tcgtcaagaa
     8221 tgtagatatt aaagccattc tcgtcttgcg tttcttcgaa gaagaactgg atgatccctt
     8281 cactagcaaa gccgtccacc accgccggta atttgacgtg attggtttca acctgcactg
     8341 acaggccgtg cagtttgtta tgcgaaatag cgccataaaa ctcgatggcg ttctccagct
     8401 tctgtaccga tacattcagg cgttcgaaga acaagcccca ggtttgacca gaaacgcgca
     8461 gcgccttgaa acgcccggtt tcctggcggg tgctggaaag gcgcagttca atacactcag
     8521 aaaccagttg ctgcacgcga gtacggatta acccgcgcag atgctggcta taacagaaga
     8581 cttccacgct atctggcggt gcggcgtcct gatgcatttt gccgagaata gttttcaggg
     8641 cttcgatcat cgattgctcg ccgttgaagt gcagcgtacg cacttcgttc cacgagttgc
     8701 ggtacagcag gtcaacgcta cctaccaggc aattttgatt ctcgccaaag ctgaagacat
     8761 ccagcttacg gaaatcaaaa tgcaccacct gattgcggaa cgccgctgtc gggtcatatt
     8821 ccaggttaac gataatcgcc agatggcgga tctcacacgg gctgtagagc gccttcggtg
     8881 tcggtgcagg taagcgcagc gggaaatggt gcgacacgtc ggcgaccatc tcctgcaact
     8941 taggcaaatc gacaatgccg ttacctttaa tatacaaacg ggtgcgcgag gtcagcagac
     9001 cgttaaacca tgcccacgcc accagtttat tcaggtaacg gttatattcc agcggctgat
     9061 ggctgatgat cgactcaata tttggcgcgc ggttatacag ataccaacct gaacggttag
     9121 cccggcccgg cggcacataa ataaaggtca gattcggttc cgagagatcg ggtgaaatct
     9181 gcgggtttac cagcgtcact ttacctggta atgcttcaaa cgcggcatac agcttacgcg
     9241 tcagcacgcc gatatcctgc ggactggcgg agacgctaag gttattgcga cgcgcaaagc
     9301 ggatcagatt acggtagctc tgcatcatcg cgtcgagcaa ctcgttgtgc gcctcacgca
     9361 cctgatcaat cttccagtta gcacggttat cgagcattgc cagacgagct tcgtcccaac
     9421 cccactcgct cactaactgg ctcaacactg cgcgacgcca gcctacacag gcgcgttcac
     9481 ggctgagttt ttcgcacact tttaaataga agcagcgacg tactaaatcc agacgggtga
     9541 aatcttcaat cgccgtcagg tattcagtaa cacgctccag catcatgcag tatggatcga
     9601 ggccaaacga tacaatctcg ccgtcgtgca aacgctgttt gatatctttc gccagcagac
     9661 gtgggttcgg gtattcccag gaataggctt ccagcagcag tgttttcagc accgctttgt
     9721 atggggaatc gatactcttg tagagctgcc aaaggctggc accaaagtac tcttcagcag
     9781 aaagcgagct taagccaccg agatccagcc attcatttgg cgtcagcacg ccctgcgcgt
     9841 aaagcgtcat cacatagtcg tcgtaatgct cttcttcgtc gcacggcacc atattccaca
     9901 gaatacgctt accggcgaga cgcacggcgg tacgataaaa ttcgtcaagc agcagtatat
     9961 gctgggtgga gccacaatct tcgcccccca ggctgccgct ttcattatga cgaaagcggt
    10021 tttcatcaat caggaagaag ctgacttcca cacccagcga ggcggcccag ctttccagca
    10081 ggctgcattt acgttgtagt aactggcgct cttcgctatc gagccaggat tgatgacaga
    10141 cccagatatc caggtcagag gaacaacttt gccctacgga cgaggtgctg cccatggtgt
    10201 atacaccagt aattggaagc tcacctttcg gtggatcctg tactgacatt ccacgataca
    10261 gttcaagctc gttcaggtag tggcgttgag tttcatcagg cgtgtaaagg caaatgcctt
    10321 tgggaacgtt accatcaagg taacccggca ttagcggatg gtgatagtgc aacaatgtcg
    10381 gcagtagact gtagacctgt tggaatgcag gccccatagc agcaagcgcg cgatccacac
    10441 gcaattgatt tatggcatcc agtctctgtt tcagagtctc aatatagagg tacaagacgt
    10501 atcgcctgat ttgctacccg tcatgactgt gattccgcca acatcaacgg taacacgcgg
    10561 cattcgggat atttcgtatg tcaaaggtaa ccgttaccac ttttcgcgcc tggttttttt
    10621 agtttcacga cgaaaaaatg gtctaaaacg tgatcaattt aacaccttgc tgattgaccg
    10681 taaagaaaga tgcgctacat acaagtgtag caccgtttat tctctgtaaa ttccttatta
    10741 cgacagcgtg aaacgtctgt caggatccac tgccagacct cattttacgg tttgcgcagg
    10801 cgtctacgtt tcaccacaac actgacatca ctctggcaag gatgttagga tggaccacgg
    10861 atgataatga cggtaacaag catgttagac aatgttttaa gaattgccac acgccaaagc
    10921 ccacttgcac tctggcaggc acactatgtc aaagacaagt tgatggcgag ccatccgggc
    10981 ctggtcgttg aactggtacc gatggtgacg cgcggcgatg tgattcttga tacgccgctg
    11041 gcgaaagtag gcggaaaagg cttatttgta aaagagctgg aagtcgcgct cctcgaaaat
    11101 cgcgccgata tcgccgtaca ttcaatgaaa gatgtgccgg ttgaattccc gcaaggtctg
    11161 ggactggtca ctatttgtga gcgtgaagat cctcgcgatg cctttgtgtc caataactat
    11221 gacagtctgg atgcgttacc agcaggcagt atcgtcggga cgtccagttt acgtcgccag
    11281 tgccaactgg ctgaacgccg cccggatctg attatccgct ccctgcgcgg caacgtcggc
    11341 actcgcctga gcaaactaga taacggcgaa tacgatgcca tcattcttgc cgtagccgga
    11401 ctaaaacgtt taggtctgga gtcccgcatt cgcgccgcgt taccacccga gatttctctt
    11461 ccggcggtag gacaaggtgc agtcggtatt gaatgccgcc ttgatgatac gcgcactcgc
    11521 gagctgcttg ccgcgctgaa tcaccacgaa actgcactgc gcgttaccgc agaacgcgcc
    11581 atgaataccc gtctcgaagg cggatgtcag gtgccaattg gtagctacgc cgagcttatt
    11641 gatggcgaaa tctggctgcg tgcgctggtc ggcgcgccgg acggttcgca gattattcgc
    11701 ggtgaacgcc gcggtgcgcc gcaaaatgcc aaacaaatgg ggatttcgct ggcagaagag
    11761 ctactgaata acggcgcgcg cgagatcctc gctgaagtct ataacggaga cgccccggca
    11821 tgagtatcct tgtcacccgc ccgtctcccg ctggagaaga gttagtgagc cgtctgcgca
    11881 cactggggca ggtggcctgg cattttccgc tgattgagtt ttctccgggt caacaattac
    11941 cgcaacttgc tgatcaactg gcagcgctgg gggagagcga tctgttgttt gccctctcgc
    12001 aacacgcggt tgcttttgcc caatcacagc tacatcagca agatcgtaaa tggccccgac
    12061 tacctaatta tttcgccatt ggacgcacca ccgcactggc actacatacc gtaagcggac
    12121 agaagattct ctacccgcag gatcgggaaa tcagcgaagt cttgctacaa ttacctgaat
    12181 tacaaaatat tgcgggcaaa cgtgcgctga tattacgtgg caatggcggt cgtgagctaa
    12241 ttggggatac cctgacggcg cgcggtgctg aggtcacttt ttgtgaatgt tatcaacgat
    12301 gcgcaatcca ttacgatggt gcagaagaag cgatgcgctg gcaatcccgc gaggtgacga
    12361 cggtcgttgt taccagcggt gaaatgttgc agcaactctg gtcgctgatc ccacaatggt
    12421 atcgtgagca ctggttacta cactgtcgac tattggtcgt cagtgagcgt ttggcgaaac
    12481 tcgcccggga actgggctgg caagacatta aggtcgccga taacgctgcc aacgatgcgc
    12541 ttttacgggc attacaataa ctctcataac aggaagccat aatgacggaa caagaaaaaa
    12601 cctccgccgt ggttgaagag actagggagg ccgtggacac cacgtcacaa cctgtcgcaa
    12661 cagaaaaaaa gagtaagaac aataccgcat tgattctcag cgcggcggct atcgctattg
    12721 ctctggcggc gggcgtcggt ttgtatggct ggggtaaaca acaggccgtc aatcagaccg
    12781 ccaccagcga tgccctggct aaccaactga cggcattgca aaaagcccag gagagccaaa
    12841 aagccgaact ggaaggcatt attaagcaac aggctacgca gcttgaacag gcgaatcgtc
    12901 agcaagaaac gctggcaaaa cagctggatg aagtccagca aaaggtcgcc accatttccg
    12961 gcagcgatgc taaaacctgg ctgctggccc aggccgattt tctggtgaaa ctcgccggac
    13021 ggaagctgtg gagcgatcag gacgtcacga ccgctgcagc gttgctgaaa agtgcagacg
    13081 ccagcctggc ggatatgaat gacccgagtc tgattaccgt tcgtcgggca attaccgatg
    13141 atatcgccag cctttctgca gtatcgcagg tggattatga cggcatcatc cttaagctta
    13201 atcagctttc aaatcaggta gataacctgc gtctggccga taatgacagc gatggttcgc
    13261 cgatggattc agacggtgaa gagctttcca gttccatcag cgaatggcgt atcaatctgc
    13321 aaaaaagctg gcagaacttt atggacaact tcattacgat tcgccgtcgt gatgacaccg
    13381 ccgtaccgct gttagcgcca aatcaggata tctatctgcg cgaaaatatt cgctctcgcc
    13441 tgctggtcgc agcacaagct gtaccgcgtc atcaggaaga gacttatcgc caggcgctgg
    13501 agaacgtctc cacctgggta cgtgcttact acgatactga tgatgccacc accaaagcgt
    13561 tcctcgacga ggtggaccag ttaagccagc aaaatatctc gatggatctt ccggaaaccc
    13621 tgcaaagcca ggcgatgctg gaaaaactga tgcagacccg cgtgcgtaac ctgctggcac
    13681 aaccggcagc ggggacaacg gaagctaaac ctgcacctgc acctgcacct gcacctgcac
    13741 ctgcacctgc acctgcacct gcacctgcac cgcaaggtga tactccggca gccgcgccgc
    13801 aaggagaata accatgctaa aagtgttatt actctttgtt ttgctgattg cggggatagt
    13861 ggttggcccg atgattgccg gtcatcaggg ttacgtgctg atccagactg acaactacaa
    13921 tatcgaaacc agcgtcacgg gcctggcgat tattttgatt ctggcgatgg tagtgctgtt
    13981 tgccattgag tggctactgc ggcggatctt ccgcacaggc gcacacaccc gtgggtggtt
    14041 tgtcggacgt aagcgtcgcc gtgcccgtaa gcagaccgaa caggcgctgc tgaaactggc
    14101 ggaaggcgat tatcagcaag ttgaaaagct gatggcgaaa aatgccgatc acgcggaaca
    14161 accggtggtg aactatctac tggctgccga agccgcgcaa caacgtggtg atgaagcacg
    14221 cgccaaccaa catctggaac gcgcagcaga gctggccggc aatgacacca ttccggtaga
    14281 aatcacccgt gtacgtctgc aactggcccg taatgaaaac catgctgcac gccacggcgt
    14341 ggataagctg ctggaagtta cgccacgcca tccggaagta ttacgtctgg cggaacaggc
    14401 gtatatccgc acaggtgcat ggagttctct gctggatatc atcccatcaa tggcgaaagc
    14461 ccatgttggt gatgaagaac atcgtgcaat gctggaacaa caggcatgga ttggcctgat
    14521 ggatcaggcg cgtgccgata acggtagtga aggtttgcgt aactggtgga aaaaccaaag
    14581 ccggaaaacg cgtcatcagg tagcgttgca ggtggcaatg gcggaacatc ttattgaatg
    14641 tgacgatcat gatactgccc agcaaattat catcgatggc ctgaaacgcc agtacgacga
    14701 tcgcctactg ctgccgattc ctcgactgaa aaccaacaat ccggaacagc tggaaaaagt
    14761 gctgcgccag cagatcaaaa acgtcggcga tcgcccgctg ttgtggagca cactgggcca
    14821 gtcactgatg aagcacggag aatggcagga agcatcgctc gccttccgcg cagcgctgaa
    14881 acaacgtccg gacgcctacg attacgcatg gcttgccgac gcgctggaca gactgcacaa
    14941 gccggaagaa gctgcagcga tgcgtcgcga cggtttgatg ttaacgttgc agaacaaccc
    15001 accacagtag ttccttctca cccggaggca agcacctccg ggacctttct gatgcataaa
    15061 aaaacgcctg ctcttattac ggagcaggcg ttaaaacagg tctgtatgac aacagtgggt
    15121 gcttcactca acgttgtgtc catggtgtct gatgaggcat aagcgacatc tgtcagtgga
    15181 cgataagcac cgtaaacggc tctgcgtcat tccggagttt atgaggcact aaggcgaaca
    15241 taagagatgg aatgagcatc tacccgttta ttatgccaca gagaatcggg aaataacatc
    15301 ccttaacact tgttatgaga taattctgta atcctctttg cttcctgagt gaatatttaa
    15361 cctgagcttg atcctacaca tacttattat gaatgataaa attcattcaa ttaataacac
    15421 atatactaat tgccgttaaa actaaaaaag cattaataat caacgcaata taataaaccc
    15481 gccttacata tcaactgcgc cagaggtagg attgaaaacg ctctcctgat tttccaattc
    15541 attttctgga taaataaata atttattttt gtcactatta tttatgtaat catcctgtca
    15601 gggagaggga tctcaattat caatgcttaa ttacgtcatc attttgatga catgaaaccg
    15661 gagcattttg catggaattt tcgttttcac ccaaacttct tgttgttgct gtcgccgccg
    15721 ctcttcctct catggccagc gcagcagata ccccgtcaac tgccaccgca cgcaaaggct
    15781 ttgccggata cgatcaccca aaccagtatc cacagaacgc tgcaatttat tgaatttgca
    15841 cgatcatgta ggccggataa ggcgtttacg ccgcatccgg caatcaaccg caggcggcca
    15901 caaaatggcc gcttttttac ttattcacca ccagcaaatg tgcctgcata atgtcactgg
    15961 ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag atatggcggt agatggcgga
    16021 aataacgctg atacccggca cacaaataat tcagccccgg tttgccgctg gcatcgagca
    16081 tgaagcggtg tttcgggcag cctccccagc acgcttttaa cacgttacaa ctgcgacact
    16141 gcgccggtaa ttgcttaaat ttatcttcac caaacacctg ctgttgcggg gaatcgatca
    16201 tttctgcaat tgtttgctga tgcatattcc ccagccgata ttgcggataa acatagtgat
    16261 cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga gcgcccacag gttggctgat
    16321 gatggcaaac cgcacccggc gcaccgacaa aattggcaaa cgcccattcg atattcatca
    16381 cgaaaatctt gccgacgtcg cgtttgatcc agtggtcgaa tatcgccacc agaaattcac
    16441 caaattcatc ggggcgcacc gaccattccg tcagttcccc ctgaatatca ccaggcgcat
    16501 gcaatttcag tccatcgcgg gcagctgttt catcagccag acgctcgacc accggaataa
    16561 actggatgaa ttcgactccc gcatcgcaca aaaaatcata aacctgcaac ggttgctgcg
    16621 cgctggtgcg gttgacgcag accagcacgt tatagtcgac atgatgtttt tgcagcagcg
    16681 tcagggcacg catcaccagc ttatgcgttg gtctgccacc tttggtcacg cgatattgat
    16741 tgtggatctc agccggaccg tccagcgata acccaacaag aaaatgattt tctgccagaa
    16801 atgcacacca tttatcatcg agcagcacgc cgttagtctg gaagctgtta cttatcttcc
    16861 tgccagcacc gtatttcgcc tgtaacttta cggcacggcg gtaaaaatcc agcccgagta
    16921 acgttggttc gccgccctgc caggtaaaag ccacttcgtt ttgcggttcg ctggcagcga
    16981 tatagtgacg gacatacgct tccagcgtgt catcgtccat atgcgtgact ggcttttcgc
    17041 ggtaaaggga ttgtttttcg agataaaaac agtagtcaca gttcagatta caatcggaac
    17101 cactcggttt cgccatcaca tgaaaagcac gcgttggaac ctgttgcagc atggtcactc
    17161 cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc ataccgtggg gcaatgagtt
    17221 attacgggcg tttattgcga agggagggtg acgaaatgca cagaaaacaa aaaaccccgc
    17281 cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga acctccgacc cactggtccc
    17341 aaaccagttg cgctaccaag ctgcgctact cgccgaaata ctgctttttg aatttttagt
    17401 tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa cccccacgtc cgtaaggaca
    17461 ctaacacctg aagctagcgc gtctaccaat tccgccacct tcgcattgtc acaacttcta
    17521 ataatggggt ggctaatggg attcgaaccc acgacaactg gaatcacaat ccagggctct
    17581 accaactgag ctatagccac cactacaaat cttgttacgc ggtattacta ccaccgcagc
    17641 tcaagcgccg ggactaaatg gcgcgcccga caggattcga acctgagacc tctgcctccg
    17701 gagggcagcg ctctatccag ctgagctacg ggcgcttagc gccgttgcgg ggggataata
    17761 cggacttccc acccctctgt ctagtccctt tttaaataaa ttgcgcgttt ggttacggtt
    17821 tgtgcgtttt gctgcttatt cctccagttt atgcgctttc ccgtggcgat taaggccaaa
    17881 aactttataa atcgccgtca ccgccagcat aaagatgatg ccaacaaaca gcgacatacg
    17941 cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc aggaatgcca ttgttacgta
    18001 gttggcccac gggaacagga ttgagcggaa cggatggctg gcaatcgccg ctttatgtgc
    18061 acgccgaaaa cgcagctggc ttatcaatat cacaaaccat ggcaccatcc ccggaagcac
    18121 gctggcactg tagacgtaga caaacacacg ctgcggattg ggaatgatgt agttcaggca
    18181 tgagccaatt agcagaatag caatagatac tgccacaccc gcaactggta cgccgtgacg
    18241 ggaaactttc gccatcgccg ccggtaactg acggtttttc gccagtgcgt agagcatacg
    18301 tccgcaactg tacatgccgc tgttacagcc agagagcgca gccgtcagca ccacaaagtt
    18361 gataatgccc gccgctgcgg taataccgat tttggcaaaa gtcagtacga acgggctgcc
    18421 gttgctgcca atttcattcc acgggaagat ggtgacgata acgaaaatcg cgcctacgta
    18481 gaaaatcagg atccgccaca gcactttgcc tacagcactg cgcagcgtca cctgcggatt
    18541 cttcgcttca ccggcagtaa tgccaatcag ctccacgccc tggtaggacg ccaccacaat
    18601 acacagcgcg gtcaggaacc ctttccagcc acccgcaaag aaaccgccat gctctgtgag
    18661 attgctaaaa ccaatcgact gcccgccatt gccaaagcca aagaaaatca cgcccaggcc
    18721 aatgacaatc atcacgataa tcgtggtgac tttgatcatc gcgaaccaga actcgatttc
    18781 gccgtacaac cgcaccgccg ccagattcgc caacgccacc agcgccaccg cgatcaatgc
    18841 aggtatccac tgcgccatct ccgggaacca gaactggaca taaacgccaa tggcggtgat
    18901 ttctgagatc cccaccgcca tccacataaa ccagtaagac caggcggtga gataaccaaa
    18961 aaacgggctc atataacgat gcgcataaac ggcgaacgaa ccggtaacag gttcaaggaa
    19021 caacatttcg cccattgaac gcatgatgaa aaagacgaac agcccggcga tgatataggc
    19081 caacaatacg gatggcccgg cccatttcag ggtactggcg gcccccataa acaggccgac
    19141 gccaatagtg ccccccaggg cgatgagttc gatatgtcga gcttccagcc cacgctgtag
    19201 ctctggtttg ttatctgcca taaatcctcg tgttgtgttt gcatgctttc cggtgttacc
    19261 ggttatcgtt atgggtacat cgagtgttgc gaatgttttc gtaattcagg agaaatggca
    19321 aataaagcat taaaaatttg aatgctttgt gtaataaaaa agcagacagg cgacggagtg
    19381 accactccgt cgctttacaa agagaggaaa atcagaggtt gccggtgtag tgccagcgta
    19441 aataacgcag caaacgaagc tgacgcttaa tgcggctcgg ctgcgaaagc aggcggtaga
    19501 gccactccag tcccagcgtt tgccagattt tcggtgcgcg ttttacgtga ccggtgaaaa
    19561 catcgtaagt cccgccaacg cccatataca gcgcatctgg atgcaccaga cggcagtcgc
    19621 gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt gacgatttgc gctccgctgg
    19681 catgaatgcg ttcaaacagc gcctgacgct gctcgggttt aaaataacca tcctgactgc
    19741 caacgatatt cacattccat tggttgcgca gtttagcttc agtttgcgcc agcacttcag
    19801 gtttaccgcc cacaagaaat accggcgtcc cttctttgcc tgcgcgcgcc atcagctctt
    19861 cccagagatc ggcaccggca acgcgggaaa cctgcgcctg cgggtacttt ttacgtactg
    19921 aacgtacaac gctgatgcca tccgcatatt taaattcggc agcgttaatt aactccctga
    19981 cctcggcgtt atcttcaatg gtcagcattt tttcagcatt aatggcaacc agcgttccct
    20041 gcttaagctg cccgtcagca aacagataat cgagggcgtg ctgcatatca cgccaaccaa
    20101 tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt gttgttattc attgttatcc
    20161 ttcaacctgc gtccggagcg atgattttgt acgtttatga atgagtccgg cgctttcaaa
    20221 aagccagtac agcagttttg cgatcatcag acatgcgccg aagaccacga taaaaaagac
    20281 tacgcgtgag acaaacgaat ccagcccttc acgcgccagc acgatcatat tgaagatcgc
    20341 cccaaagcag aaactgtgca atatcgcagc cttatagcga ttaggctcgc ggttgcccag
    20401 ctcatacagc cagtcgaacc atttgatgat cagaccaacc acgatcgccc cgagcgggat
    20461 gaacaacgcg ccgcccatca ccaccagtga gcctataagc gtaggcgaga tcgccagtcc
    20521 ggagtggtta ttcagcactt cccaggtaaa gtagttggct gagttcagca ccatactcgg
    20581 gcgacccggc cacagccagg aagggataaa gacatagaaa tcgcggacaa ttggagccag
    20641 gccctggaag tcgatgttgt cgtagttctg caacagcaac gccagattct cccacgggga
    20701 gaaggtgtcg cgagtgagat agagaaacgt atagaacgct tcatcgccgc tcacattcat
    20761 tccatagcgt tttagcgcca gccagaacat gccaacaatc cccagcacgc ccgccgccgc
    20821 cagcatccac aacgaaatcc agccgcgaat aatgccaata aacaggaaga tagcgaatgc
    20881 gatgatgata ttggcgcgag tgccgccgac aatcatataa gtcagcaagc caaaggcgac
    20941 cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg cgcagaaagt agaccaccag
    21001 catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc acgccggaga cttcactgga
    21061 gaagatctga ctgtaggagt taagccggaa cagcaaaaag ccgttgtgca tgaagaagat
    21121 gccgacgctt accagcgcga tacccatcag gatcacccac gtaagattgg tctccacgcg
    21181 gttcatggta aacagcggac ggcgcggtac atcagcaacg cgtttgcgta ggcgggtttt
    21241 gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa agcaacgcct gcaacaagat
    21301 ttctggcggc gcgacaccaa catcaaagcg aaataccagc acgctggtca gcgggaagcc
    21361 gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca ttgaagttaa agcgcacacg
    21421 gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg cagagcagcc agaccacaaa
    21481 caggccactg aattgcagca gactcatgcg acctccccgg cggcaatagc caacgcccgc
    21541 tgccagcctt gtagatagtt agggctaaag aaggcaatgg tgtttttatc caccgacgcc
    21601 aactggcgct gcgcttcacg cacaatatcc tcgttgagat cgtcggtagt aaacagcacc
    21661 ggcaaatgct gttccgtcat atcctgccag aacggatttt cccggttaag cacacaagga
    21721 atgcccgcct gaatcagtaa gcaaagcgta ccaatgccct gctggcgggc aaaaataaag
    21781 taaccaagat cgcactgacg aagtagcgcc agataggcgt caaattccag tttttcgctc
    21841 aaaatttgca gattttcttc gctgaataac tccagccccg cctgacgaac ttcctcaatg
    21901 tacgcttcgt tattaggcgg atatcccatc ggcaccacca cttttaccgt atcgccaaat
    21961 tgctgatgaa cggcgcgcaa ggcagcaata tggtcattgc tgcggtcacc ggagttcccc
    22021 accagaatgg tcattttccc ttcacgttgc cgatcattcg ccatcgtatt gagagaaggg
    22081 tccatccgcg tcgggaagta cagcagttcg ccccgcacct ttggatgcgt tttggcaaaa
    22141 aagctcaaat caccgcgggt ggcaaataca cagccgactc gcttttgcgc caggcgacgt
    22201 agcgggtaaa aaagcttata tctcaagcca ctggaaagct catacaggtc tgccccccag
    22261 atatgccaga aaaactggct gggcttaatg ccaccactca gcagagccag ccacagtgtg
    22321 ggattgaact gaccgtggaa aaaaaatcgc tgctgacggt tagcttttgc tttcgcgatg
    22381 accgcttccg ccagcgattt tttcccaggg aaaaattgca cagaaagcgc cggacagcta
    22441 tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc gcgcgtgctc gctcgtcgcg
    22501 gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat ggtgagggat atccgatccc
    22561 agtacgtgaa tcagtacagt catgcccgcc tacgccagag taaaaacacg ccacaacaaa
    22621 gagaaaaata gacgatataa gttgccatat atgcctgcgc cgcgcccagt gcaccatgcg
    22681 cagggattag ccagtgggca aataccatca ataaagtgaa ctggctgact tccgccagaa
    22741 tataaaaccg cagtgacgct ttggcgatca ccagataacc aaagacataa gcgcccactt
    22801 ttaacacatc acccactaac tgccaggcaa agagatcgcg catagcggta aatttattcg
    22861 acaacagcaa ccagatagca aaatcacgca gcaaccagac ggtaaaactc gccgccgcca
    22921 ctgccggtaa gacgaatttc agcgatttaa ccacttcccg ggtgatatcg cgcttttccg
    22981 ttagccgcga caacgtgggc agcaaatata cgctgaacga tgccgtaata aattgcaggt
    23041 aggcatcgga aatactgctc accccttgcc agatccccac ttcatcccag ctatactgcg
    23101 ccgccagcag tttacgcatc atgatgtaag caacaggcaa ggtcaccgac gtaatcaacg
    23161 ccatgagcgt aaatttgctc aactgccctg ccagaccgtt atcccagctg ggtttcagat
    23221 agcttaacgg gatgacacca cgtttgatca acatgatggc ggcaggaatc accaccagcg
    23281 cgggaatcag cgccagaccc agcaacgccc cttcataacc gcccaaacgg taactgacgt
    23341 agtacgcgag aacgccaatc aagctgccga caatcagcga taacgcatta cctgcggcat
    23401 cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca ggcgatcccc atttgcacca
    23461 gcgccactaa acgcaccagc ccctgatagt cggtattacc aaacagtccc tggctgattg
    23521 gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga gaaaccaagt accatcgctg
    23581 atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc atggtactgg gcaacgtatt
    23641 tggttacacc gttaaagatg ccagctccgg caagcacgcc gagcacggta atcaactggc
    23701 ggaaatttgc cgccagccca agccccgccg gaccaaatga cacagccagc aacttaccga
    23761 ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc cgtccacaag gacgcttttg
    23821 ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa ttaccgtacg ctgattgacg
    23881 ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct cgctctcttt ggtggtgtag
    23941 cgatcttcac cgtggaactc accaaagcgt tcccccgcag ggcaaccgtg cagcggaatg
    24001 taatggaaca ccgccatgat ttccgcttct ttcagaaagt taatcaacgc gctccggtca
    24061 tcaatatccc gcagtttaat gtagaacata tgcgcgttct gcacgcagcc atcgggaatc
    24121 gacggcagct cgatacgccc ggctttcgcc agaggcgcta acgcatcgta gtagttttgc
    24181 cacagcgcca gacgttgctg gttgatacga tccgctgctt ccagttgcgc ccacaggtac
    24241 gcagcttgca gatcggacat caaatagctg gagccaatat cgcgccaggt atatttatcg
    24301 acctgaccac ggaagaactg gctgcggtta gtgccctttt cacggatgat ctcggctcgt
    24361 tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt caccacccgc cgtatagttt
    24421 ttggtttcat ggaagctaaa gcagccaata tgaccaatgg ttcccagtgc acgccctttg
    24481 taagtggaca tcacgccctg agcggcatct tctaccacaa acagattatg ctttttcgcc
    24541 aacgccataa tggtgtccat ttcgcaggcc acacccgcgt aatggaccgg cacgataacg
    24601 cgcgttttgt cggtgatcgc cgcttcaatc agcgtttcgt cgatgttcat ggtgtccggg
    24661 cgaacatcca caaaaacgat ttttgcgcca cgcagcacaa aggcattggc ggtggagaca
    24721 aaggtgtagc tcggcatgat cacttcatcg ccaggctgga tatcgagcag cagcgccgcc
    24781 atctccagcg aagcggtgca ggacggcgtc agtaacactt tggcgctgcc aaaacgttgc
    24841 tccagccact gctggcagcg acgggtaaaa ccgccatcgc cacacagttt gccgctaccc
    24901 attgccgact gcatatagtc gagttcggtt cccaccaccg gcggtgcgtt aaatggaatc
    24961 atgtgatcac ctgtataacc agtacgcggt gctttctaca ttcgcaccac tttgtatgta
    25021 tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc cgcaaagttg ttttaccgcg
    25081 agcatacgcc cagtttagcg ccgtttgcat cagctcagca cctgcaccgc gtccagccag
    25141 caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa gagacataac cgcgaatacc
    25201 gccggacgcc gcacgtaaaa tcagacattg atgatcaaag gtgccgcgca cggcattttc
    25261 aatccactgt gcataaaagc gtccgctggc gtcaggcgca taccacggcg cacgaaaacg
    25321 gctttgcgca aatgcggcgc tggctaactg acgtaatgcg ggaatatcgg tctcttgtgc
    25381 cactacagca ccactatcac tgacattgtt cacgggtagt gccaaatcaa cttcaccttc
    25441 taccagggag aatcccagct gttgcagggc atccagttca cccgtatttg atgccgcaat
    25501 tttggcctgc acccgtgacc acggcgctaa cacgtctggc gtcaggagct gtgcttcaga
    25561 cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg ttttcccagg ttagtggctc
    25621 aatactggcg cggacgggca cgaagtaact ccagcagata ttggccgtag ccagttttcg
    25681 ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag ccagccgtta cgccaggcga
    25741 tctcttccag gcaggcaatc ttaaagccct ggcgtttttc tactgtctgc acaaaggtac
    25801 tggcttcaat caggctgtcg tgagtgccag tgtccagcca ggcaaatccg cgcccgagca
    25861 gttcaacggt caggttgccc gcctcgaggt acatctggtt gatggaggta atctccagtt
    25921 caccacgctc cgacggcttc acctgctttg cgtactccac gactttactg tcgtagaaat
    25981 aaagcccggt caccgcccag tttgacttcg gctgttttgg cttttcttcc agcgagatag
    26041 cgcggaaatt gtcgtcaaac tccaccacgc caaagcgttc cgggtccatc acctgatagc
    26101 caaataccgt cgccccttcc gtgcgcgccg caacatgacg cagcttcgga ctgaagccct
    26161 gaccgaagaa gatgttatcg cccagcacca gacaagaagg ttcaccgttg aggaaggttt
    26221 caccgatgat aaaggcctgc gccagaccgt ccgggctggg ctgttcggcg tattccagct
    26281 ggataccgaa ctcactacca tcgcccagca ggcgctggaa ataaccttta tcttccggcg
    26341 tagtgatgat gagaatttcg cggatatcgg ccaacatcag caccgacagc ggatagtaaa
    26401 tcattggctt atcgtaaatc ggcaacagtt gcttcgatac gccgcgcgta atcggatgca
    26461 atcgggtgcc ggaaccgccc gccaggataa tacctttcat gtttgcctcc gctgaaaatt
    26521 agcctttcag acctaaacgc tcgccctgat agctgccgtc ctgcacctgc ttccaccagc
    26581 tttcattagc cagataccac tgcaccgttt tacgcattcc actttcaaag gtttcctgtg
    26641 gcagccagcc aagttcacgg gcaattttcg aagcatcaat ggcataacgc agatcatgcc
    26701 ccggacggtc agcgacaaag gtgatcaagt cacgatagtg caccacgccg tgcggcttgt
    26761 tcggagccag ttcttccagc agctcgcaaa tggtttccac aacatcgaga ttcttacgct
    26821 cgttgtgacc accaatatta taggtttcac cgactttccc ggtggtcgcc acgcaataca
    26881 gcgcgcgggc gtgatcttcc acatacagcc agtcacggat ttgctgcccg ttgccatata
    26941 ccggcagcgg tttacccgcc agcgcgttga ggatcatcag cgggatcagt ttttccggaa
    27001 agtggtaagg accgtagtta ttcgagcagt tggtgataag cgtcggcaga ccgtaggtcc
    27061 gcagccaggc gcgcaccaga tggtcgctgc tggcttttga cgcggaataa gggctgctcg
    27121 gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga gtgcaggtca ccatatactt
    27181 cgtcggtgga gatatgatga aaacggaacg ctgatttttt atcttccgtc agcgtattcc
    27241 agtaagcccg cgccgcttca agcaatgtat aagtcccgac aatgttggtt tcaataaacg
    27301 ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc tgccagatgc atgacacagt
    27361 ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg atcgcagata tcaactttct
    27421 caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga catcaggttt ccggcgtagg
    27481 tcagcttatc gaccactacc accgcgtcgc tcgtttcgtt gatgatataa cgcaccagcg
    27541 ccgagccaat aaacccggca ccacctgtta tcagaatttt tctcatcagc gccagactcc
    27601 tttggcatcg acgacatact gctgatggac attgtcgcca ttgataactt taaactgact
    27661 atgatcgacc agcatcacca gcacgtctgc cgttgccagc gcctcgtcaa gctgcgccag
    27721 agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg ttaggctcaa caaccagagt
    27781 ttcgccgcta tgccactggg cgatcagttc agcgatttcc atcgccgggc tttcgcgcag
    27841 gtcatcaata ttcggtttaa acgccagacc aaagcaggcg attttcagtt cactggcgcg
    27901 tttatcggta gccgccaggc aatcagccac cgccgctttc acctgatcga taacccagaa
    27961 cggtttgtga tcgttcactt cgcgcgcggt acggataagc cgcgcctgct gggggttctg
    28021 tgccacgata aaccacggat caacagcaat gcagtgaccg cccacgccag ggccaggctg
    28081 aagaatatta acgcgagggt gacgattcgc caggcgaatc agttcccaga cgttaatccc
    28141 ctgatcggca caaatcagcg acaattcatt agcaaaagcg atattcacat cgcggaagct
    28201 gttttcggtg agcttacaca tttccgccgt ccgcgagtta gtgacgacac actcaccttc
    28261 gaggaaaatt ttgtacagtt cgctggcgcg ggtcgaacaa accggcgtca taccaccaat
    28321 cacgcgatcg tttttaatca gctcgaccat aacctggccc ggtaacacgc gttccgggca
    28381 gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt gggaaagtga gatccggacg
    28441 catctctgct aaccattctg ccatcttctc ggttgacccc accggcgagg tggattcaag
    28501 gatcaccagc gcgccttttt tcagcactgg cgcaatggag cgagcagccg attcaacgta
    28561 ggtcatatct ggctcatgat cgcctttaaa cggcgtgggt acagcaatca gccaggcatc
    28621 cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct tctacggcag tttttactac
    28681 gctcgccaaa tcaggctcga cgatatggat ttcgccacga ttgatggtat caaccgcgtg
    28741 ttggttgata tcgacaccaa tcacctgttt ttgccgtgag gcaaacgctg ctgctgtagg
    28801 cagcccgatg taacccagtc cgataacaga aatggtcgca aaactcatag tgatatccga
    28861 ttatttttta acgcttccag aatgcgagag catgcctgac catcaccata cgggttatgg
    28921 gcgcggctca tagtttgata ttcgttttcg tcttttaaaa gacgcgtcac ttcctcgaca
    28981 attcgctgct tatccgtgcc taccagacgc accgtacccg ccgtcaccgc ttccggacgc
    29041 tcagtggtat cgcgcatcac cagcaccggt ttccccagcg aaggcgcttc ttcctgaatg
    29101 ccgcctgagt cggtcaaaat cagccaggcg tggttcatca gccagacaaa cggtaaatac
    29161 tcctggggat cgatcagaat gacatttttc acatgcccca gaatgcgatt gaccggctct
    29221 ctgacgttcg ggttgagatg caccggatag acaatctgga tgtcctggtg cgtggtggca
    29281 atgtctgcca acgcgtggca gatttcttca aagccacgac cgaaactttc acgcctgtga
    29341 ccggtcacca gaatcatctt tttatcgggg tcgataaacg ggtaatttgc cgccagttct
    29401 gaacgcagcg tatcgctgct catcacctgg tcacgcaccc ataacagtgc atcaataact
    29461 gtattaccag taatgaagat tcggctatcc gcaacgtttt cacgcagcaa gttttgccgg
    29521 gaagtttcgg ttggagagaa gtgatacatc gccagatgcc cggtcaatgt acggttagcc
    29581 tcttccggcc acggcgaata aagatcgccc gtgcgcagac cagcctcaac gtgaccaaca
    29641 ggaatacgct gataaaacgc cgccaggctg gttgccagcg tcgtcgttgt gtcgccgtga
    29701 accagcacga cgtctggttt gaactcggca agaataggtt ttagcccttc cagaatccga
    29761 caggttatct ctgtcaggcc ctgtcctggc tgcattatgt tgagatcgta gtcaggtaca
    29821 atggaaaaga gtttcagcac ctgatcgagc atctcccgat gctgcgcagt gacgcaaact
    29881 ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca acggcgccat cttgatggct
    29941 tccgggcgcg taccaaatac agtcagtact ttcacatcga ttctcttcga ataagcggcg
    30001 agcgcctttg cgctcaccgc agcagtgttg ctatttcgag caacggcggg ttaatgcgac
    30061 accagccccg atcagccccc cgacaatgcc ccacataatc atcaggaagg cacgacgtgg
    30121 gctatcgcgt tttaccggtt cttccggcgt acgcaaatag cgataggtct gaaaacgcgg
    30181 atccagggtt ggaccaacat tcagggtgtt taacatggcc cgattctgat catagtcgag
    30241 atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga gcctggagca ttggacgccc
    30301 aagcaggaac atttctgaat caggtaattc ctcggcaggc acatctgtcg cactgcgcga
    30361 aatattatgc tgctcagcaa ttttcagcgc ctgttcaata ctgttcatcc ggcggtcgta
    30421 gatggctttc gccacctctt cctgacgctt cacctgtgct ttcatctgga tggtacgcgc
    30481 cgcccatgcg cctttcagct catcattcag atggctggct gcacgctggc tggcaaaagc
    30541 aacatactga cgtaacaggt tattagcgtc aggcgcggtt tcagcaataa gcttcacgct
    30601 gtcattgacc gcgcgggtaa agtctccggg gataaactgg atgttgttaa tcatttcatc
    30661 cagcaacgcc gcatcggctt tgctgttgcc caccatccgc tgtttgtaat agtcggtttg
    30721 cagccagaac tctctgcggg tatcccacga ggccagttgc ataacaaact ctttataggc
    30781 ttcgtccatg accgatggtt ggtcggcaga agccatgttt gaacggacat ccaggttacg
    30841 caaaaattgc tgctgcgagt aatatccccc cagcatattc accgttggac gatcggtaat
    30901 cgccgtcgag ctccactcct gacgagcaaa aaaagtatac gccagcgcga ttaacgcaaa
    30961 tgccagcccc atgccaataa tccatagctt cccagcccac aaggtacgaa acaacccacg
    31021 aatatccagt tcattttcag cgtcttcggc cggtttccca ggcattggtt gtgtcatcac
    31081 atcctcattt atttggttaa attggggctg ccaccacgat ttctacgcag tctgcgtttt
    31141 acgcgcttaa taaagcgagc aactttccag gcacgcttaa tgcaatatcc atagaggaag
    31201 aatgctagca aaaagagcac cagcatgacc cactccggga caaaatgaga atattctgcc
    31261 agcacgccaa tggaagcgag cagtgccgca gcaagggtaa tcagcacaaa cgcctgacgg
    31321 gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct gacggtcagg agagaatggg
    31381 ctcatgcctt tacgcaggcg acggtacata atcgccacca tatccattag cggaatggcg
    31441 attatccaca aagcggtaac cgggctgatg ggatgggttt tgccctgggt cgtttcgagc
    31501 aggatccaga taacggtaaa accaatcagc gtactgcccg catcacccat aaagactttg
    31561 tagcggcgac ccaggatacc aaggttaagc atgatgtatg gcaggatggc ggcgatcatc
    31621 gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc acaaaatcat accgattgct
    31681 gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa tgccatcaac catgttgaac
    31741 gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt aaccaaacgg tccgagcacc
    31801 atctcccagg agccaaagat ataacccagg ctactgagat aaagtttgcc gaacaccatc
    31861 ataacaatgc caacagcggc ctgtatggtg gcacggattt ttacgctgat atcaaaacgg
    31921 tcatccagcg cgccaatgaa aacaagcaca ccggcacaag cgagatagag agatgcatgc
    31981 ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc ctgcgtaaac cgaaatcccc
    32041 ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt ttggtttatc cactaaaccg
    32101 actttttttg ccaccttacg ggcaaaaaac agaaacagtg tcgtgaataa aaaaatactg
    32161 atgagatcag tactcactgt cagtaaattc acaatgcatg ctctcagaga aaattattag
    32221 cagaagtata accacgaaga cctttattca gaagggaaag tccgttccga acccaccagt
    32281 cctgtgcaaa aattaaccat ttgatatgca aattgcataa cacgccagca aattccagga
    32341 attttcctaa catcgccagc gcggcattaa gattacagat ataaaagcaa aacgccacgt
    32401 aaacacgtgg cgtttttggc ataagacaaa tttatgagcg tttcatcatt tcgaagaaat
    32461 cgtcattggt cttggtcatg gccagtttat taatgaggaa ttccattgca tcgatttcgc
    32521 ccatcgggtg aatgattttg cgcaggatcc acattttctg cagttcttcc tgagtagtga
    32581 gcagttcttc tttacgggta ccggaacggt tgtagtcgat agccgggaag acgcgttttt
    32641 cagcgatctt acgagagagg tgcagttcca tgttgcctgt acctttaaac tcttcgtaga
    32701 taacttcgtc cattttagaa ccggtatcga taagcgccgt cgcgataata gtcaggctgc
    32761 cgccctcttc cacgttacgc gccgcaccaa agaagcgttt cggacgatgc agggcgttgg
    32821 catccacacc accggtcaac actttacctg acgccggaac aacggtgttg taagcgcgcg
    32881 ccagacgagt gatggagtcg agcagaatga taacgtcttt cttgtgctca accaggcgtt
    32941 tggccttctc gatcaccatt tccgcaacct gaacgtggcg agatgcgggt tcgtcaaagg
    33001 tagaagcaac aacttcacct tttaccagac gctgcatctc ggttacttct tccggacgtt
    33061 cgtcgatcag cagaaccatc agcacacaat ccgggtggtt gtaagcaatg ctctgggcaa
    33121 tgttctgcag cagcatggtt ttaccggctt tcggcggtgc cacaatcaga ccacgctgac
    33181 cacgaccgat aggtgatgcc agatccagta cgcgagcagt taaatcttca gtagaaccgt
    33241 taccacgttc catacgcaga cgagaatttg cgtgcagcgg ggttaagttc tcaaagagga
    33301 ttttgttgcg ggcgttttca ggtttgtcga agttaacttc gttaactttc agcagcgcaa
    33361 aatagcgttc accttctttc ggcgggcgaa tcttaccaga gatggtatca ccagtgcgga
    33421 ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc atcaggaccg gcgaggtagg
    33481 agctgtctgc ggaacggagg aaaccaaatc catcctgcaa tatctccagt acgccatcac
    33541 caaagatatc ttcgccactc tttgcgtgct gcttcaggat ggcaaaaata atgtcctgct
    33601 tacgcatgcg agccaggttt tccagcccca tattttcgcc gagagtgatc agctcagaaa
    33661 ccggcgtatt ctttaattcg gtaagattca tagtggtgtg agttcttaaa cttggggtaa
    33721 atctcgaact taatgttgtg aatggtatgg caggatcatc catgcctgtt tacggtcatc
    33781 gactcatgtc ttttcgctgc ctggtcacag gagagtacgc agaactgaaa cgacaagacg
    33841 gattgagtga cgagccagaa atctgttcac ttcgcattta agataaaacg ggaagcgttg
    33901 gaaattacaa gattcaaact taataaggta tgtttaatac gaagtcaaca ctaagttagc
    33961 atgactcacg ccgggcgtcc agtttttagc gacggggcac ccgaacatga aattccctta
    34021 cgccaggtta gcgtcgagga actctttcaa ctgaccttta gacagtgcgc ccactttggt
    34081 tgccgccact tcaccgtttt tgaacagcag cagagtcggg ataccacgga tgccatattt
    34141 cggcgcagtg ccagggtttt gatcgatgtt cagttttgca acggtcagtt tgccctgata
    34201 ttcgtcagcg atttcatcca gaatcggggc gatcattttg cacggaccgc accactctgc
    34261 ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca tccgtgtcaa aactgtcgtc
    34321 agtcaggtga ataattttat cgctcatata taactccaca ggaataagcc tggcgtgttg
    34381 gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac tttatttcac cggatacgct
    34441 ttcgtaaagc aatagtaagc tgatattcta ccacactatg agcaaaacac atttaacaga
    34501 acagaagttt tccgacttcg ccctgcatcc gaaggttgta gaagcccttg aaaaaaaagg
    34561 gtttcataac tgtacgccca ttcaggcact ggcccttccg ctgacgctgg cgggtcgtga
    34621 cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg gcgtttctta cgtcaacgtt
    34681 tcattatctt ctctctcatc ctgcgattgc cgatcgcaag gtgaatcaac cgcgtgcctt
    34741 aattatggca ccgacgcgtg aacttgccgt gcagatccat gccgacgcag aaccgctggc
    34801 ggaagctact ggcctgaagc tgggtctggc ttacggtggt gatggctacg acaaacagct
    34861 gaaagtgctg gaaagcggcg ttgacattct gattggcacc acgggtcgtt taattgacta
    34921 cgccaagcag aaccacatta acctcggtgc cattcaggta gtggttctgg atgaagccga
    34981 tcgcatgtac gatctgggct ttattaaaga tatccgctgg ctgttccgcc gtatgccgcc
    35041 tgcaaaccag cgcctcaaca tgctgttctc cgccacgctt tcgtaccggg tacgtgaact
    35101 ggcgttcgag cagatgaaca atgccgaata tattgaagtg gaaccggaac agaaaacggg
    35161 ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa gaaaaaatgc gtttgctgca
    35221 aacgctgatc gaagaagagt ggccagacag agcgattatt ttcgccaata ccaaacaccg
    35281 ttgtgaagag atctggggcc acctggcggc agatggtcat cgtgtcggtt tgttgacagg
    35341 cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa tttacccgtg gcgatctgga
    35401 tattctggtt gccaccgacg ttgccgcgcg tggtttgcat attccggcag tgacgcacgt
    35461 ctttaactac gatttacccg atgactgtga agattacgtt caccgtattg gtcgtacagg
    35521 tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt gaagagtatg cattgaattt
    35581 gcctgctatt gaaacctata ttggtcactc aattccggta agcaaataca atccggacgc
    35641 attgatgacc gatctgccaa aaccgctgcg cctcacgcgc ccacgtacag gcaatggtcc
    35701 gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt taaataaaat gtcatactca
    35761 aaatcattca aactgtatca agatggcaag agaatgaatc ccgatgagca tactgtagta
    35821 agtgattcag gtgaaagaat gcagccaaca cagagacaga ttgaaggatg aagagtatgg
    35881 gttccacctc gtcgctgtat gcagccattg atctcggttc gaatagtttt catatgctgg
    35941 ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc
    36001 tggctgctgg cctgaatagc gaaaatgccc tgtctaatga agcaatggag cgcggttggc
    36061 aatgtctgcg cctgtttgct gaacgtctgc aagatatccc tccctcgcaa attcgcgttg
    36121 tcgctacggc gacattacgt cttgccgtca atgcgggtga ttttattgcc aatgcgcagg
    36181 aaatcctcgg ttgtccggta caggtgatca gcggtgaaga ggaagcacgt ctgatttatc
    36241 agggcgttgc tcacacaact ggcggtgccg atcagcgcct ggtggtggat ataggcggtg
    36301 ccagtactga actggtaacc ggcacgggtg cacaaaccac ctcgttgttc agcctgtcga
    36361 tgggctgcgt cacctggctg gaacgctatt ttgccgatcg taatctgggg caggaaaatt
    36421 ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat gaattacggt
    36481 atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt gcaggcgtta caggaaatca
    36541 tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa ttgaaacagc
    36601 gagccattca ttgcggtcgg ctggaggaac tggagattga cgggctgacg ctggaacgtg
    36661 cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat ttttaccgaa ctgaatattc
    36721 agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggc atgttgcatc
    36781 tggccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcagcgc cgctttatga
    36841 tcgatattga tcaggcgcag cgcgtagcca aagttgcggc taacttcttc gatcaggtgg
    36901 aaaaggaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc tgtcagcttc
    36961 atgaaatcgg cctgagcgtt gactttaaac aagcgccgca acacgctgct tatctggtgc
    37021 gtaatctgga tcttcccggt tttacccccg cacagaaaaa attgctggcg acgctactgc
    37081 tcaaccagac taatccggtc gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc
    37141 gcgtcgcaga acaactctgc cgtttactcc gcctggccat catttttgcc agccgtcgcc
    37201 gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg accttgacgc
    37261 ttccgcaagg ttggctaacc caacatccgc tgggtaaaga gattattgct caggaaagcc
    37321 agtggcagag ctatgtccac tggccgctgg aagtgcatta atgagtaagt gccggatgcg
    37381 atgctgacgc atcttttccg gccttgatta tttccctcgt tttgccgcca tcatcgcttt
    37441 cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga ccactttgcg
    37501 ctcctgttcc caaatcagat catcctgcgg cagctccagc aaaaagcggc tcggctccgg
    37561 gcgcaccagc tcgccgtact gacggcgttc tttacacagc gtaaaggtca attccttctg
    37621 ggcgcgggta atgccgacat aggccagccg ccgctcctca tcgatattat cttcatcgat
    37681 gctgctctgg tgcggcaaaa acccttcttc cataccgacc atgtagacat aaggaaactc
    37741 cagccctttt gacgcgtgca gagtcatcag ttgcacctga tccagctctt cttcactctc
    37801 accacgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg
    37861 ctcatccagt tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt
    37921 cttcatgcgc atttcggcgg ctttcgggct gggcgatgtt tcgtacagcc aggattcata
    37981 atccatgccg tggatcagat cgcgcaccgc ggcaatcggc tcccgctccg ccagacgctg
    38041 gatttctgcc aaccagtgag taaagcgggt caatgcttca taaccacgtc cgctaagcgt
    38101 ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg tcatcgccca
    38161 ctcacccagc tttttcagcg tcgccgggcc aatctctctc ttcggcgtgt taacgatacg
    38221 cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc agataagcca gcaagtcctt
    38281 gatttcagga cgagagaaaa acgacgtacc accagaaatt ttgtacggga tgcggttttg
    38341 catcaggaat ttttcaaaca ctcgcgactg atggttaccg cgataaagaa tagcgtaatc
    38401 tttgtactgc gttttattga cgaagtgatg cgcaatcagt tcaccagtga cgcgctcggc
    38461 ttcatgttct tcgttattcg cgcttaaaac tttaagctct gtgccgtaac ccagttcgga
    38521 gaacagacgc ttctcaaaga cgtgcgggtt attggcgatc aggatgttcg ccgctttcaa
    38581 aatacgtccg gaagagcggt agttctgctc aagcttaatc accttcagcg ccggaaaatc
    38641 ctgactcagc agcaccaggt tttgtggacg tgcgccgcgc caggagtaga tcgactggtc
    38701 gtcatcgccc accacggtaa agcgcgcgcg gctgcccacc agcagtttca ccagttcata
    38761 ctggctggtg ttggtgtcct gatactcatc caccagcaga tagcgaattt tgttctgcca
    38821 gcgctcgcgg acttcttcat tgcgttgcag cagcaacgtc ggcaataaaa tcagatcatc
    38881 gaagtcgaga acattgcacg ctttcaggtg tgcatcatac agcccataac aatgggcaaa
    38941 aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg gacggtgttt tgagatcgtt
    39001 cttccagtta gagatggtcg aaatcaactg ttgcaggaga actttgtcat cttcaatcag
    39061 cccttcggtc aactctttga gcaacgccag ctgatcggta tcgtcaaaca gcgaaaagtt
    39121 cgccttcatc cccagcgccg catactcacg cttgatgata tccagcccca gcgtgtggaa
    39181 ggttgagatc atcagcccac gcgcctcttt gcgacccagc gtctgtccga cacgctcttt
    39241 catctcgcgc gccgccttat tggtaaaggt caccgccgcg atatgtcgcg cctgataacc
    39301 gcaaccgcgg atcagatggg cgattttatt ggtgatcaca cgagttttac cggaacccgc
    39361 gcccgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt ggccggggtt
    39421 tagacgcata ggtgtattgc tcaatcttcg aacgggggga ggattgtagc agaatcgcat
    39481 ttgaatgtga ccgcgtgtaa gccgctaacc gattggctgc ggatgtttaa atcagagagg
    39541 gaagaccgaa accggagttc atcgtgaatc cattagactt agaaaatatc gggtcgtaac
    39601 gttgtgttat tactttttca gagtttacta aggagaggtg tactcgtggc gattaatttc
    39661 agtcctaagg ttggtgaaat actggattgc aactttggta actatcctgt tagccagaat
    39721 ggacagttca gcaccacgta ttacgatggc cgcattcctc ctgaaatgat aaaaaacagg
    39781 ttagt
//
LOCUS       DAEEBB010000019        39644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEEBB010000019 DAEEBB010000000
VERSION     DAEEBB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.019"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="KLF07_000969"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="KLF07_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBD9831051.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="KLF07_000970"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="KLF07_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBD9831052.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="KLF07_000971"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="KLF07_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBD9831053.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="KLF07_000972"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="KLF07_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBD9831054.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="KLF07_000973"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="KLF07_000973"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBD9831055.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="KLF07_000974"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="KLF07_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBD9831056.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="KLF07_000975"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="KLF07_000975"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBD9831057.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="KLF07_000976"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="KLF07_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBD9831058.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="KLF07_000977"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="KLF07_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBD9831059.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="KLF07_000978"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="KLF07_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBD9831060.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="KLF07_000979"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="KLF07_000979"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBD9831061.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="KLF07_000980"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="KLF07_000980"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD9831062.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="KLF07_000981"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="KLF07_000981"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBD9831063.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="KLF07_000982"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="KLF07_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBD9831064.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="KLF07_000983"
     CDS             complement(14282..14581)
                     /locus_tag="KLF07_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBD9831065.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="KLF07_000984"
     CDS             complement(14793..15233)
                     /locus_tag="KLF07_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD9831066.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="KLF07_000985"
     CDS             15334..15933
                     /locus_tag="KLF07_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBD9831067.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="KLF07_000986"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="KLF07_000986"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBD9831068.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="KLF07_000987"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="KLF07_000987"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBD9831069.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="KLF07_000988"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="KLF07_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBD9831070.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="KLF07_000989"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="KLF07_000989"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBD9831071.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="KLF07_000990"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="KLF07_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBD9831072.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="KLF07_000991"
     CDS             complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="KLF07_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBD9831073.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21877..22575
                     /gene="cpxR"
                     /locus_tag="KLF07_000992"
     CDS             21877..22575
                     /gene="cpxR"
                     /locus_tag="KLF07_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBD9831074.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22572..23945
                     /gene="cpxA"
                     /locus_tag="KLF07_000993"
     CDS             22572..23945
                     /gene="cpxA"
                     /locus_tag="KLF07_000993"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBD9831075.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="KLF07_000994"
     CDS             complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="KLF07_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBD9831076.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="KLF07_000995"
     CDS             complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="KLF07_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBD9831077.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="KLF07_000996"
     CDS             complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="KLF07_000996"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBD9831078.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27022..28056
                     /gene="rhaT"
                     /locus_tag="KLF07_000997"
     CDS             27022..28056
                     /gene="rhaT"
                     /locus_tag="KLF07_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBD9831079.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="KLF07_000998"
                     /pseudo
     CDS             complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="KLF07_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="KLF07_000999"
     CDS             complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="KLF07_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBD9831080.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30099..31568
                     /gene="rhaB"
                     /locus_tag="KLF07_001000"
     CDS             30099..31568
                     /gene="rhaB"
                     /locus_tag="KLF07_001000"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBD9831081.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31565..32824
                     /gene="rhaA"
                     /locus_tag="KLF07_001001"
     CDS             31565..32824
                     /gene="rhaA"
                     /locus_tag="KLF07_001001"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBD9831082.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33066..33890
                     /gene="rhaD"
                     /locus_tag="KLF07_001002"
     CDS             33066..33890
                     /gene="rhaD"
                     /locus_tag="KLF07_001002"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBD9831083.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33900..34214
                     /gene="rhaM"
                     /locus_tag="KLF07_001003"
     CDS             33900..34214
                     /gene="rhaM"
                     /locus_tag="KLF07_001003"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBD9831084.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34515..34961
                     /locus_tag="KLF07_001004"
     CDS             34515..34961
                     /locus_tag="KLF07_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBD9831085.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34972..36501
                     /locus_tag="KLF07_001005"
     CDS             34972..36501
                     /locus_tag="KLF07_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBD9831086.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            36491..37561
                     /locus_tag="KLF07_001006"
     CDS             36491..37561
                     /locus_tag="KLF07_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD9831087.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37561..39309
                     /locus_tag="KLF07_001007"
     CDS             37561..39309
                     /locus_tag="KLF07_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBD9831088.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39358..>39644)
                     /locus_tag="KLF07_001008"
     CDS             complement(39358..>39644)
                     /locus_tag="KLF07_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HBD9831089.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9624 a        10301 c        10303 g         9416 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcgtgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacagcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgccca
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agctaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccacgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactccca ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg
    21181 tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg
    21241 agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca
    21301 actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca
    21361 ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg
    21421 ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc
    21481 gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca
    21541 taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc
    21601 tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt
    21661 cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta
    21721 tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt
    21781 caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc
    21841 tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat
    21901 gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg
    21961 attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt
    22021 ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag
    22081 acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc
    22141 ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg
    22201 gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca aaacaacgac
    22261 aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg tcaggaagcc
    22321 agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg
    22381 gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa
    22441 cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg tcgtaaactg
    22501 ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg
    22561 gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc
    22621 tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg
    22681 agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc gaagcggagc
    22741 tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata
    22801 agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg
    22861 ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc
    22921 atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg
    22981 gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca
    23041 ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg gtcagtacgc
    23101 cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg
    23161 ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg
    23221 aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga
    23281 cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc
    23341 tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctcgagcgta
    23401 ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta
    23461 atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg tggagtgaag
    23521 tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc
    23581 cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca
    23641 ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata
    23701 aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg
    23761 aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa tctggcggta
    23821 caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg
    23881 cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga
    23941 gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag
    24001 gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc
    24061 cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc
    24121 gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc gtcatcaaac
    24181 ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc
    24241 aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata caaccacccc
    24301 accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt
    24361 ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca gcggaaaata
    24421 tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc gccaaacgca
    24481 ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag atagtgctca
    24541 cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg
    24601 tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat tttggcaatt
    24661 gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat
    24721 cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg
    24781 cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg acgaacgtta
    24841 gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta aaatttcgat
    24901 tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat
    24961 cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca tcggtttaaa
    25021 cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga
    25081 agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg
    25141 aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac cagtctgggc
    25201 aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa tcagcgtttg
    25261 cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg caaagcccac
    25321 caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt
    25381 gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc
    25441 agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat
    25501 atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa cttcaacacc
    25561 atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt
    25621 taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc
    25681 ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt
    25741 gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa
    25801 tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc
    25861 catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga gcatcagtgt
    25921 tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac cacatcaatt
    25981 gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa
    26041 ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac gcctcattgc
    26101 agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg tcccagttca
    26161 ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc aggtagtaag
    26221 catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa atagcttcac
    26281 ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg cctttcagca
    26341 ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga
    26401 agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc tgcagggtgg
    26461 tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc
    26521 gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg
    26581 gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct
    26641 gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat
    26701 cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt attgtcgggc
    26761 ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa
    26821 aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat
    26881 gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg gcggcaacag
    26941 gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg tgaagcgacg
    27001 aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt ttggcatttg
    27061 atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa aaaatggtca
    27121 tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc gtggaccatc
    27181 agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct ctctacgctg
    27241 ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta tggcctgacc
    27301 atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac gttgattgtc
    27361 ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa caccgaaggc
    27421 ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgtagggat tgtaactcga
    27481 gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa tctgaaaaaa
    27541 gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac
    27601 gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact gtatgtcgct
    27661 ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg tttctgtttt
    27721 attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg
    27781 ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta tctgcaattc
    27841 tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat cagttggatg
    27901 ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg
    27961 aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc
    28021 gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt gagatgacgc
    28081 cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata gttactatct
    28141 tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg gagaagatat
    28201 tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc agtctgctgg
    28261 cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa tttatccagc
    28321 gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag caacgtttcg
    28381 ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa catcaccaac
    28441 tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact actttcatgc
    28501 tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt
    28561 ggttgccctg cgctggcgct aaatcccgga atcgctccct gtcagtcaag attcagcttc
    28621 agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga agcgtaggag
    28681 tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata aggacgatcg
    28741 ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa atcatgtgta
    28801 tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg ctggtcgctg
    28861 gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac ctcggccaga
    28921 gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt cgcgttattg
    28981 cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa
    29041 aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt cagtaacgct
    29101 ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg
    29161 ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga
    29221 aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct ccagccaggc
    29281 cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg
    29341 cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt
    29401 ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca atacgctgtg
    29461 gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca actgattcag
    29521 cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca gacacagatt
    29581 atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg tgccaccggt
    29641 gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga caatcacaat
    29701 ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg gttctatcgc
    29761 cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca tactggcctc
    29821 ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc ttaaattttc
    29881 gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat
    29941 gcggtgagca tcacatcacc acaatgaagc taattgtgaa catcatcacg ttcatctttc
    30001 cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt
    30061 tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc aattgtgtcg
    30121 ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc
    30181 gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc cagaacggtt
    30241 atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta aacaaggtgt
    30301 gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg gactttgtgc
    30361 tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat agccgctcca
    30421 atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat caacgtagcg
    30481 gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg gagcaacaac
    30541 ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc agctatcgcc
    30601 tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg gtcaatatca
    30661 atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa gcctggtttg
    30721 gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag ggtaatgaga
    30781 ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc tcgccgttaa
    30841 acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc ttcgaaagcc
    30901 agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa ggcggggcgg
    30961 aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag cgagtgcttc
    31021 aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca cttccggcct
    31081 gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg atgtgcagcg
    31141 aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat gctgaactgg
    31201 cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc
    31261 agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc cagaacacgc
    31321 tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg cctgttgaag
    31381 cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc aacaatgtgg
    31441 atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc cctaatcctg
    31501 acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca aaggagcttt
    31561 gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt
    31621 ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt caatgcactg
    31681 ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga ccggtgggat
    31741 tcaggctatt ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac gagccgatct
    31801 ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg ccatctatct
    31861 ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca aaaactgggt
    31921 tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt tctcgcatcc
    31981 gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc agttctggat
    32041 tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc
    32101 atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg accgtctcgc
    32161 cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc tggatccggc
    32221 gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga gctacacggt
    32281 tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc tgtgcctgga
    32341 cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg ccatgctgta
    32401 tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg atcacgtggt
    32461 gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg atctgttcga
    32521 ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg ccgcgtgggt
    32581 cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga
    32641 gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac tggaagaaca
    32701 gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg atacgccagc
    32761 aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg
    32821 gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga atgaccatgc
    32881 gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa acatagcctc
    32941 gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg gggagagggt
    33001 tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt acaggaacac
    33061 agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg
    33121 atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg
    33181 acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat attcccctca
    33241 gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct
    33301 tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg
    33361 gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact tccgaacttc
    33421 cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga
    33481 tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg
    33541 cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg
    33601 gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac
    33661 aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt
    33721 cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag
    33781 tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc
    33841 tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa ggagcaaaca
    33901 tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc
    33961 ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact
    34021 acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg
    34081 aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg aaatatatga
    34141 ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg caagaagtgt
    34201 tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat tttaaatttc
    34261 gatttgaaaa attaaacata ttacatctcc cggtaagtta tatttccctg atacattgtg
    34321 agtaaatcac aaaaataatg aataacccat taatgattca tgtggtttat ttaaataacc
    34381 attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt cattgtttac
    34441 cgacatatcg cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt
    34501 atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat attcagggca
    34561 atggcgctta ttccgttctg aagcagttgg cgacaatagc gttacaaaac ggttttatca
    34621 ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat
    34681 ttggttccgg tgtcgccgtg ccgcacggta aaagcgcctg cgttaaacaa ccgtttgtat
    34741 tattcgcccg caaagcgcag gctattgact ggaaagccag cgatggcgaa gacgtcaatt
    34801 gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa atcatcggca
    34861 cactgtgtcg caaaattatc caccaggatt ttattcatca actgcaacag ggcgacgccg
    34921 accaggtgct tgccttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca
    34981 tccttatgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg
    35041 gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa
    35101 gggtccagtg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc
    35161 attctcgcta ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc cgggaagaaa
    35221 gtttatgaga ttgccatctc ccaggcgttg aaaaatatcg accagatttt cagcgaatta
    35281 ccgacaaact cgcagctttt tgccgcagat agcggcgtga agcttggcaa acaggaagtg
    35341 caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccgtttgtc
    35401 atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccgtat
    35461 accgatatgt cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat cggttacctt
    35521 ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa
    35581 ccggctttcg ctccggcgtt tctggtttgc tatctggcga acgacaaagc gctgctcggc
    35641 acccagtcgg gtgcgggatt cctcggcgcg gtggtgtttc tggtttgcta tctggcgaac
    35701 gacaaagcgc tgctcggcac ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg
    35761 ctggcgattg gctatttcgt cttctggttc cgcaaagtgc gccttggcaa agcattacaa
    35821 ccgctgctcg gttcaatgct gatcccgttc gttactctgc tggttttcgg cgtgctcact
    35881 tactacgtta tcgggccggt gatgtccgac ctcatgggcg ggctgctcca cttcctgaat
    35941 accattcctc catcaatgaa gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc
    36001 gatatgggcg gccccatcaa taaaaccgcc tggttcttct gcttctcact gctggaaaaa
    36061 cacatttatg actggtacgc catcgtcggc gttgtcgcgt tgatgccgcc cgttgcggcg
    36121 ggtcttgcga cgttcattgc gcccaaactg tttacccgac aggaaaaaga ggctgccagt
    36181 agtgccattg tggtcggtgc cacagtcgca accgaaccgg ctatacctta cgctctggca
    36241 gctccgttgc cgatgattac cgccaatacg ctggcgggtg gcattaccgg cgtgctggtg
    36301 atcgctttcg gaatcaaacg tctggcacct ggtttgggta tctttgaccc gctgatcggc
    36361 ctgatgtcgc cggtgggttc gttttatctg gtgctagcga ttggcctcgc gctgaacatt
    36421 tcattcatta tcgtcctgaa aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag
    36481 gagcttgtcc atgaacattg agttactgca acagttgtgc gaagccagcg ccgtcagcgg
    36541 cgatgaacag gaagttcgcg acattctgat aaacacgctg gaaccttgcg ttaatgagat
    36601 cacctttgat ggtctgggca gctttgttgc ccgtaagggg aataaaggtc caaaagttgc
    36661 cgttgtcagg catatggatg aagtcggctt tatggtcacc cacatcgacg agagcggttt
    36721 tctgcgcttt accaccattg gcggctggtg gaatcagtca atgctcaacc accgggtaac
    36781 catacgcaca cacaagggat ttaaaatccc tggtgtgatt ggttccgtcg cacctcatgc
    36841 gttaacggaa aagcaaaagc aacagccgct gtcatttgat gagatgttca ttgatattgg
    36901 cgcgaacagt cgcgatgagg tggaaaaacg cggtgtggcg atgggcgatt ttattagccc
    36961 ggaagccaat tttgcctgct ggggcgaaga taaagtggtc ggcaaggcgc tggataaccg
    37021 catcggctgc gcaatgatgg ctgaactatt gcagacggtg aataatcccg aaattacgct
    37081 gtatggcgtc ggcagcgtgg aagaagaagt tgggctacgc ggggcacaaa cctcggcgga
    37141 acacattaaa ccggatgtgg tgattgtgct ggataccgct gtcgcaggtg atgttccggg
    37201 cattgataac attaaatacc cgctgaaact gggccagggg ccggggctga tgctgtttga
    37261 caagcgctac ttccccaacc agaaactggt ggcggcgtta aaaagctgtg ccgcacataa
    37321 cgatttaccg ctgcaatttt ccaccatgaa aaccggtgcg acggatggcg ggcgctacaa
    37381 cgtgatgggc ggcgggcgtc cggttgttgc gctgtgcctg ccaacccgct atctgcacgc
    37441 taacagcggc atgatttcaa aagccgatta cgatgctctg ctcacgttga tacgggattt
    37501 tctgaccacc ttaaccgcgg agaaagtcaa cgcgtttagc cagttccgtc aggtggatta
    37561 atgctaaacg aacgccagtt aaagattgtc gatctgctgg agcaacagcc gcgcacgcct
    37621 ggtgagctgg cgcaacagac tggcgtttca ggcaggacca tcctgcgtga tattgactat
    37681 ctcaacttta cccttaacgg caaagcccgc attttcgcca gtggcagtgc gggctatcag
    37741 ctggaaatct tcgagcgccg cagctttttt cagttgctgc aaaagcacga taacgacgat
    37801 cggctgctgg cgctgttatt actgaatacc tttactcccc gtgcgcaact cgcctcggcg
    37861 cttaatttgc cagaaacctg ggtagcagag cgtctgcccc ggttaaaaca gcgttatgaa
    37921 cgcacttgtt gcctggccag tcgccccggt ttgggccatt tcattgatga gacagaagag
    37981 aaacgcgtta tcttgctggc gaacttgctg cgcaaagatc cgtttttaat tccgctggcg
    38041 ggcgtaacac gagacaattt tcagcattta tccacggcct gcgacaacca acaccgctgg
    38101 ccgctcatgc agggtgatta tctctccagc ctgattctgg cgatttacgc cctgcgtaat
    38161 caactgaccg atgagtggcc gcaatatccc ggtaacgaga taaaacaaat cgttgaacag
    38221 agcggcatgt ttcttggtga taacgctgta agaaccctga caggtttgat agagaaacag
    38281 catcagcaag cgcaggtaat ttcagccgat catgtgctgg ggttgctgca aagggtgcca
    38341 ggcatcgcgt cattgaatat tattgatacg cagctggttg agaatattac cgggcattta
    38401 ttacgttgcc ttgccgcacc ggtgtggatt gccgagcacc gccaaagcag catgaataac
    38461 ctgaaagccg cctggcctgc ggcctttgat atgagtctgc actttattac gctactgcgt
    38521 gaacagctcg atattcccct tttcgatagc gatctgatcg gtttgtattt tgcctgtgcg
    38581 ctggaacggc atcaaaacga acgccagccg atcattttgc tctcggacca gaacgcgatt
    38641 gccactatta atcagctcgc cattgagcgc gatgttttac attgtcgggt gattattgcc
    38701 cgtagcttaa gcgagcttat tgccattcgc gaagagattg agccgttatt gatcattaac
    38761 aacagccatt atttactgga tgacgcggta aataattaca tcaccgtaaa aaatatcatt
    38821 acggctgccg atatcgaaca aataaaacac tttctggcga cggcatttat tcgccaacag
    38881 ccggaacgct ttttctctgc ccccggaagt tttcattatt cgaatgtacg cggtgaaagc
    38941 tggcaacata ttacccggca aatttgtgcg caattagtgg cacaacacca tattaccgcc
    39001 gatgaagcgc aacggatcat cgcccgcgaa ggcgaaggtg aaaacctgat tgttaatcgc
    39061 ctcgccatcc cacattgctg gagcgaacag gagcgacgtt ttcgtggatt ttttattacc
    39121 ctcgcccaac cagttgaggt gaataacgaa gtcattaacc atgtcttgat cgcctgcgcc
    39181 gccgccgatg cgcgtcatga gctaaaaata tttagctatc tggcaagcgt attgtgtcag
    39241 catccggcag aagttattgc cgggttaaca ggatatgagg catttatgga gttacttcac
    39301 aaggggtgaa ctgtgtcgga tgcgccgcat ccgacactgc attttcatca aaaacgttta
    39361 acgcaggcta ttatagtcat caaccatttc gttgtactcg cgtaacgctc gtggaaatga
    39421 gtcttcgacc atccagcttg tgtttgcatc ctgaagatgc tctttatccc agtcagagta
    39481 cgggacttta tctctgacgc ggcgaacata ttttttagtc tcgagttgat actggtctgc
    39541 cgaattaatg aacgagaaat tcataccgcc tttatccgct tctttggcct gcgctaccag
    39601 cgtttccagt tcagagactt tcttcattgc cgcatcaaca tcaa
//
LOCUS       DAEEBB010000020        39477 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEEBB010000020 DAEEBB010000000
VERSION     DAEEBB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.020"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="KLF07_001009"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="KLF07_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBD9831090.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="KLF07_001010"
     CDS             1946..2230
                     /locus_tag="KLF07_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBD9831091.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="KLF07_001011"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="KLF07_001011"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBD9831092.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="KLF07_001012"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="KLF07_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBD9831093.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="KLF07_001013"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="KLF07_001013"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBD9831094.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="KLF07_001014"
     CDS             7236..7799
                     /locus_tag="KLF07_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD9831095.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="KLF07_001015"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="KLF07_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBD9831096.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="KLF07_001016"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="KLF07_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="KLF07_001017"
     CDS             11269..11817
                     /locus_tag="KLF07_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831097.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="KLF07_001018"
     CDS             11814..12284
                     /locus_tag="KLF07_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831098.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="KLF07_001019"
     CDS             12281..12805
                     /locus_tag="KLF07_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831099.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="KLF07_001020"
     CDS             12792..13664
                     /locus_tag="KLF07_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBD9831100.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="KLF07_001021"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="KLF07_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBD9831101.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="KLF07_001022"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="KLF07_001022"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD9831102.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="KLF07_001023"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="KLF07_001023"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBD9831103.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="KLF07_001024"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="KLF07_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBD9831104.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="KLF07_001025"
     CDS             17383..18192
                     /locus_tag="KLF07_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBD9831105.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="KLF07_001026"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="KLF07_001026"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBD9831106.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="KLF07_001027"
     CDS             18774..19052
                     /locus_tag="KLF07_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBD9831107.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="KLF07_001028"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="KLF07_001028"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBD9831108.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="KLF07_001029"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="KLF07_001029"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBD9831109.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="KLF07_001030"
     CDS             22833..24011
                     /locus_tag="KLF07_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831110.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="KLF07_001031"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="KLF07_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBD9831111.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="KLF07_001032"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="KLF07_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBD9831112.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="KLF07_001033"
     CDS             26304..27317
                     /locus_tag="KLF07_001033"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD9831113.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="KLF07_001034"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="KLF07_001034"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBD9831114.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="KLF07_001035"
     CDS             28212..28871
                     /locus_tag="KLF07_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD9831115.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="KLF07_001036"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="KLF07_001036"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBD9831116.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="KLF07_001037"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="KLF07_001037"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBD9831117.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="KLF07_001038"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="KLF07_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBD9831118.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="KLF07_001039"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="KLF07_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBD9831119.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="KLF07_001040"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="KLF07_001040"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBD9831120.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="KLF07_001041"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="KLF07_001041"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBD9831121.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..35944
                     /gene="argT"
                     /locus_tag="KLF07_001042"
     CDS             35162..35944
                     /gene="argT"
                     /locus_tag="KLF07_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBD9831122.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36165..36947
                     /gene="hisJ"
                     /locus_tag="KLF07_001043"
     CDS             36165..36947
                     /gene="hisJ"
                     /locus_tag="KLF07_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBD9831123.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37037..37723
                     /gene="hisQ"
                     /locus_tag="KLF07_001044"
     CDS             37037..37723
                     /gene="hisQ"
                     /locus_tag="KLF07_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBD9831124.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37720..38436
                     /locus_tag="KLF07_001045"
     CDS             37720..38436
                     /locus_tag="KLF07_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9831125.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38444..39217
                     /gene="hisP"
                     /locus_tag="KLF07_001046"
     CDS             38444..39217
                     /gene="hisP"
                     /locus_tag="KLF07_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBD9831126.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9348 a         9672 c        10613 g         9844 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatactg caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    35641 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc
    35701 gttacaagat gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt
    35761 cgcctttgct ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    35821 gctacgtaaa gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg
    35881 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    35941 ctgatacgtc gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt
    36001 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    36061 tctaacgggg cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa
    36121 acctgtaaat attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc
    36181 tatcgctctc tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc
    36241 aaaacatccg catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag
    36301 gcgaactggt tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc
    36361 aatgtacgtt tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga
    36421 ttgacgccat catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca
    36481 ccgacaaact gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc
    36541 cgacagtcga gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga
    36601 cgttcggtaa tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg
    36661 acaacattta ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg
    36721 ctgccagcga aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt
    36781 ctgttaaaga tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata
    36841 acgaactgcg cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg
    36901 agaaattagc gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga
    36961 aagaaagccc gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca
    37021 cgacaggaca ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt
    37081 cacgctggag ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc
    37141 tggcggtaag ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct
    37201 gattcgtggc gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc
    37261 gctaaacacg gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc
    37321 tggtattatc actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc
    37381 ttttatggca gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg
    37441 gcaagtgttt cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg
    37501 caacaactgg caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga
    37561 tgtggtcaaa gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat
    37621 cgtctgtggc gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct
    37681 tgagcgccgc tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa
    37741 tactggaaac cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg
    37801 tggctgctta ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt
    37861 cgtgtctcca gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc
    37921 ggtacgccgc tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt
    37981 gttaagggaa ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg
    38041 gcgctgacgc ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg
    38101 gttccgcatg gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat
    38161 cgttgcatta ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg
    38221 atcctgatgc tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa
    38281 atagcccgcg atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg
    38341 gtgctctatt taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc
    38401 tggttgcagc atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt
    38461 aaacgttatc gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact
    38521 gcaagcgaat gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac
    38581 ctttctgcgc tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg
    38641 ccagacgatc aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca
    38701 actgcgctta ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca
    38761 tatgacggtg ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca
    38821 ggaagcgcgc gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca
    38881 ggggaaatat ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc
    38941 gctggcgatg gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga
    39001 actggtaggc gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt
    39061 ggtagtgact cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca
    39121 tcaggggaaa atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc
    39181 tcgtctgcaa cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct
    39241 gccggagtat tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag
    39301 tccgctaaaa atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt
    39361 gttaatcgtg gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa
    39421 tatcgacaac ttccacgccg catgatgcgg tatttaaatc ttttttacgc catccag
//
LOCUS       DAEEBB010000021        39407 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEEBB010000021 DAEEBB010000000
VERSION     DAEEBB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.021"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..266
                     /locus_tag="KLF07_001047"
     CDS             <1..266
                     /locus_tag="KLF07_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBD9831127.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="KLF07_001048"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="KLF07_001048"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBD9831128.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="KLF07_001049"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="KLF07_001049"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBD9831129.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="KLF07_001050"
     CDS             complement(1570..3270)
                     /locus_tag="KLF07_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBD9831130.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="KLF07_001051"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="KLF07_001051"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBD9831131.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="KLF07_001052"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="KLF07_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBD9831132.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="KLF07_001053"
     CDS             4828..6600
                     /locus_tag="KLF07_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD9831133.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="KLF07_001054"
     CDS             6593..8374
                     /locus_tag="KLF07_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD9831134.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="KLF07_001055"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="KLF07_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBD9831135.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="KLF07_001056"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="KLF07_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBD9831136.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="KLF07_001057"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="KLF07_001057"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBD9831137.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="KLF07_001058"
     CDS             11336..11908
                     /locus_tag="KLF07_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBD9831138.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="KLF07_001059"
     CDS             complement(11939..12250)
                     /locus_tag="KLF07_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBD9831139.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="KLF07_001060"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KLF07_001060"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="KLF07_001061"
     CDS             12629..12982
                     /locus_tag="KLF07_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBD9831140.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="KLF07_001062"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="KLF07_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="KLF07_001063"
     CDS             complement(14736..15206)
                     /locus_tag="KLF07_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBD9831141.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="KLF07_001064"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="KLF07_001064"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBD9831142.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="KLF07_001065"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="KLF07_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD9831143.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="KLF07_001066"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="KLF07_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBD9831144.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="KLF07_001067"
     CDS             complement(17208..20357)
                     /locus_tag="KLF07_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBD9831145.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="KLF07_001068"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="KLF07_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBD9831146.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="KLF07_001069"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="KLF07_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBD9831147.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="KLF07_001070"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="KLF07_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBD9831148.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="KLF07_001071"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="KLF07_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBD9831149.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="KLF07_001072"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="KLF07_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBD9831150.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="KLF07_001073"
     CDS             26836..27213
                     /locus_tag="KLF07_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBD9831151.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="KLF07_001074"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="KLF07_001074"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBD9831152.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="KLF07_001075"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="KLF07_001075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBD9831153.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="KLF07_001075"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="KLF07_001076"
     CDS             30024..30353
                     /locus_tag="KLF07_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBD9831154.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="KLF07_001077"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="KLF07_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBD9831155.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="KLF07_001078"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="KLF07_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBD9831156.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="KLF07_001079"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="KLF07_001079"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBD9831157.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="KLF07_001080"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="KLF07_001080"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBD9831158.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="KLF07_001081"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="KLF07_001081"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBD9831159.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="KLF07_001082"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="KLF07_001082"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBD9831160.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="KLF07_001083"
     CDS             complement(37406..39082)
                     /locus_tag="KLF07_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBD9831161.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9526 a        10048 c        10418 g         9415 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgatttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatg
//
LOCUS       DAEEBB010000022        39258 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEEBB010000022 DAEEBB010000000
VERSION     DAEEBB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.022"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="KLF07_001084"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="KLF07_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBD9831162.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="KLF07_001085"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="KLF07_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBD9831163.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="KLF07_001086"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="KLF07_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBD9831164.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="KLF07_001087"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="KLF07_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBD9831165.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="KLF07_001088"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="KLF07_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBD9831166.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="KLF07_001089"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="KLF07_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBD9831167.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="KLF07_001090"
     CDS             complement(6574..7338)
                     /locus_tag="KLF07_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD9831168.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="KLF07_001091"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="KLF07_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBD9831169.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="KLF07_001092"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="KLF07_001092"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBD9831170.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="KLF07_001093"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="KLF07_001093"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBD9831171.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="KLF07_001094"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="KLF07_001094"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBD9831172.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="KLF07_001095"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="KLF07_001095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBD9831173.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="KLF07_001096"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="KLF07_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBD9831174.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="KLF07_001097"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="KLF07_001097"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBD9831175.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="KLF07_001098"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="KLF07_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBD9831176.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="KLF07_001099"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="KLF07_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBD9831177.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="KLF07_001100"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="KLF07_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBD9831178.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="KLF07_001101"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="KLF07_001101"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBD9831179.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="KLF07_001102"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="KLF07_001102"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBD9831180.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="KLF07_001103"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="KLF07_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBD9831181.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="KLF07_001104"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="KLF07_001104"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBD9831182.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="KLF07_001105"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="KLF07_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBD9831183.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="KLF07_001106"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="KLF07_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBD9831184.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="KLF07_001107"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="KLF07_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBD9831185.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="KLF07_001108"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="KLF07_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="KLF07_001109"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="KLF07_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBD9831186.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="KLF07_001110"
     CDS             complement(31717..33003)
                     /locus_tag="KLF07_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD9831187.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="KLF07_001111"
     CDS             complement(33054..33995)
                     /locus_tag="KLF07_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBD9831188.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="KLF07_001112"
     CDS             complement(34010..34780)
                     /locus_tag="KLF07_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBD9831189.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="KLF07_001113"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="KLF07_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBD9831190.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="KLF07_001114"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="KLF07_001114"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBD9831191.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39258
                     /gene="caiB"
                     /locus_tag="KLF07_001115"
                     /pseudo
     CDS             38069..39258
                     /gene="caiB"
                     /locus_tag="KLF07_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9397 a        10790 c        10279 g         8792 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcgaatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcag
//
LOCUS       DAEEBB010000023        38336 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEEBB010000023 DAEEBB010000000
VERSION     DAEEBB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.023"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            59..1423
                     /locus_tag="KLF07_001116"
     CDS             59..1423
                     /locus_tag="KLF07_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831192.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="KLF07_001117"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="KLF07_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBD9831193.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="KLF07_001118"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="KLF07_001118"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBD9831194.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3673
                     /gene="yajL"
                     /locus_tag="KLF07_001119"
                     /pseudo
     CDS             3084..3673
                     /gene="yajL"
                     /locus_tag="KLF07_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
     gene            complement(3727..5175)
                     /gene="thiI"
                     /locus_tag="KLF07_001120"
     CDS             complement(3727..5175)
                     /gene="thiI"
                     /locus_tag="KLF07_001120"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBD9831195.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5381..5623
                     /gene="xseB"
                     /locus_tag="KLF07_001121"
     CDS             5381..5623
                     /gene="xseB"
                     /locus_tag="KLF07_001121"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBD9831196.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5623..6522
                     /gene="ispA"
                     /locus_tag="KLF07_001122"
     CDS             5623..6522
                     /gene="ispA"
                     /locus_tag="KLF07_001122"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBD9831197.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6547..8409
                     /gene="dxs"
                     /locus_tag="KLF07_001123"
     CDS             6547..8409
                     /gene="dxs"
                     /locus_tag="KLF07_001123"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBD9831198.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8464..9438
                     /gene="yajO"
                     /locus_tag="KLF07_001124"
     CDS             8464..9438
                     /gene="yajO"
                     /locus_tag="KLF07_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBD9831199.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9492..10010)
                     /gene="pgpA"
                     /locus_tag="KLF07_001125"
     CDS             complement(9492..10010)
                     /gene="pgpA"
                     /locus_tag="KLF07_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBD9831200.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9988..10965)
                     /gene="thiL"
                     /locus_tag="KLF07_001126"
     CDS             complement(9988..10965)
                     /gene="thiL"
                     /locus_tag="KLF07_001126"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBD9831201.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11043..11462)
                     /gene="nusB"
                     /locus_tag="KLF07_001127"
     CDS             complement(11043..11462)
                     /gene="nusB"
                     /locus_tag="KLF07_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBD9831202.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11482..11952)
                     /gene="ribE"
                     /locus_tag="KLF07_001128"
     CDS             complement(11482..11952)
                     /gene="ribE"
                     /locus_tag="KLF07_001128"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBD9831203.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12041..13144)
                     /gene="ribD"
                     /locus_tag="KLF07_001129"
     CDS             complement(12041..13144)
                     /gene="ribD"
                     /locus_tag="KLF07_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBD9831204.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13148..13597)
                     /gene="nrdR"
                     /locus_tag="KLF07_001130"
     CDS             complement(13148..13597)
                     /gene="nrdR"
                     /locus_tag="KLF07_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBD9831205.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13748..14287
                     /locus_tag="KLF07_001131"
     CDS             13748..14287
                     /locus_tag="KLF07_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD9831206.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14586..15470
                     /gene="tsx"
                     /locus_tag="KLF07_001132"
     CDS             14586..15470
                     /gene="tsx"
                     /locus_tag="KLF07_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBD9831207.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15647..15994)
                     /locus_tag="KLF07_001133"
     CDS             complement(15647..15994)
                     /locus_tag="KLF07_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBD9831208.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16123..17094)
                     /gene="secF"
                     /locus_tag="KLF07_001134"
     CDS             complement(16123..17094)
                     /gene="secF"
                     /locus_tag="KLF07_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBD9831209.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17105..18952)
                     /gene="secD"
                     /locus_tag="KLF07_001135"
     CDS             complement(17105..18952)
                     /gene="secD"
                     /locus_tag="KLF07_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBD9831210.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18980..19312)
                     /gene="yajC"
                     /locus_tag="KLF07_001136"
     CDS             complement(18980..19312)
                     /gene="yajC"
                     /locus_tag="KLF07_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBD9831211.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19335..20462)
                     /gene="tgt"
                     /locus_tag="KLF07_001137"
     CDS             complement(19335..20462)
                     /gene="tgt"
                     /locus_tag="KLF07_001137"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBD9831212.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20518..21588)
                     /gene="queA"
                     /locus_tag="KLF07_001138"
     CDS             complement(20518..21588)
                     /gene="queA"
                     /locus_tag="KLF07_001138"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBD9831213.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21681..22262
                     /gene="acpH"
                     /locus_tag="KLF07_001139"
     CDS             21681..22262
                     /gene="acpH"
                     /locus_tag="KLF07_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBD9831214.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22267..24084)
                     /gene="malZ"
                     /locus_tag="KLF07_001140"
     CDS             complement(22267..24084)
                     /gene="malZ"
                     /locus_tag="KLF07_001140"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBD9831215.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24240..25613)
                     /gene="proY"
                     /locus_tag="KLF07_001141"
     CDS             complement(24240..25613)
                     /gene="proY"
                     /locus_tag="KLF07_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBD9831216.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25689..27008)
                     /gene="brnQ"
                     /locus_tag="KLF07_001142"
     CDS             complement(25689..27008)
                     /gene="brnQ"
                     /locus_tag="KLF07_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBD9831217.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27415..28710)
                     /gene="phoR"
                     /locus_tag="KLF07_001143"
     CDS             complement(27415..28710)
                     /gene="phoR"
                     /locus_tag="KLF07_001143"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBD9831218.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28768..29457)
                     /gene="phoB"
                     /locus_tag="KLF07_001144"
     CDS             complement(28768..29457)
                     /gene="phoB"
                     /locus_tag="KLF07_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBD9831219.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29647..30849
                     /gene="sbcD"
                     /locus_tag="KLF07_001145"
     CDS             29647..30849
                     /gene="sbcD"
                     /locus_tag="KLF07_001145"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBD9831220.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30846..33989
                     /gene="sbcC"
                     /locus_tag="KLF07_001146"
     CDS             30846..33989
                     /gene="sbcC"
                     /locus_tag="KLF07_001146"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBD9831221.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34115..35299
                     /gene="araJ"
                     /locus_tag="KLF07_001147"
     CDS             34115..35299
                     /gene="araJ"
                     /locus_tag="KLF07_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBD9831222.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35442..36350)
                     /gene="mak"
                     /locus_tag="KLF07_001148"
     CDS             complement(35442..36350)
                     /gene="mak"
                     /locus_tag="KLF07_001148"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBD9831223.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36475..37386
                     /gene="rdgC"
                     /locus_tag="KLF07_001149"
     CDS             36475..37386
                     /gene="rdgC"
                     /locus_tag="KLF07_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBD9831224.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37464..37919)
                     /locus_tag="KLF07_001150"
     CDS             complement(37464..37919)
                     /locus_tag="KLF07_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831225.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37916..38251)
                     /locus_tag="KLF07_001151"
                     /pseudo
     CDS             complement(37916..38251)
                     /locus_tag="KLF07_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8823 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gcgaggtggc tgatggcgaa tatgacgtga tcgtactgcc
     3301 tggtggcatt aaaggcgcag agtgttttcg cgatagtacc ctgctggttg aaaccgttaa
     3361 acagttccac cgttccgggc gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct
     3421 ggtaccgcac gatatcttcc cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa
     3481 aattcccgcc gaacaatggc aggacaagcg cgtcgtctgg gatgcacggg taaaattgct
     3541 gaccagccag gggccgggta cggctattga ttttggtctg aaaattatcg acctgctggt
     3601 tgggcgcgag aaaacccatg aagtggcatc acaactggtg atggcggcag ggatttataa
     3661 ttattacgag tagtgtcgga tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac
     3721 cacaaattat gggcgataca ccttcacatt gttaaagccc tgctcgcgca gatagagcgc
     3781 ctgcagacgg ctcatcaccc cgcgctcaca ccacagtagc caggttctgt tctggtcgag
     3841 atcgccaaat ttggtgctca gtttatagaa cggcagagaa accacgtcga taccttcaac
     3901 cttcagcggc ttatcttcct gctcatcgac agaacggata tcgaggatca cgtcgttcgg
     3961 gccaaagcca ttgacggttt ccacttccac cacttcctgc tcggtctgct gggcgatttc
     4021 gcggatatca acgttattcg cttcctcaac cactttatcg agaatactga agtcgaactt
     4081 ctcttcttcc gcttcaatct tcgatttaac cgctttcacc gtcgggcttt tggagatcac
     4141 gccacaatat tctggcatcg tgcgagcaaa gtcttcagtg ccaatctggc gggccaggtt
     4201 gatgatgtgc tctttgtcgt aagaaatcag cggacgcagg atcagcgtat cagagacgtt
     4261 atcaatcagg cgcaggttgg tcagcgtctg actggacacc tggccgagcg cttcgccggt
     4321 gaccagcgcc tgtacgccgt aacgttcagc cactttagac gcggcacgca ccatcatacg
     4381 tttgaggata acgcccatct gaccgtcgtc gattttctcg agaatttccc cgacgaccgg
     4441 ttcgaaatta atggcgacaa aacgcacgcg gtgggagctg ccaaagcggt tccacagata
     4501 atgcgccacc tgacgcacgc caatttcatg cgctgcgccg ccgaggttaa agaagcagta
     4561 atgcacgcgg cagccgcgac gcatcaacat ataactggaa acaccggagt cgaaaccacc
     4621 ggaaatgagc gacagcacat cttcctgggt gccaatcggg aaaccgccaa taccttcgta
     4681 gcggccttta atcagcagaa gacgatcgtc ttccacttcc agatggacag ttacatccgg
     4741 gttagtcagc ttcacgcgcg cggattcaat atgctgattt aaaccgccgc cgacgtaacg
     4801 ttccacatca atcgagctaa aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt
     4861 tttgccttcc agctgatcgc gatattgaac caacgctttc tcgaaaatat cgtgcatgtc
     4921 ggtaaacggc acgtcttcga cttcgagaat atggtggata cccggaatac gggtcagggc
     4981 gtcgcgaata gccagacgct gtttttcatc ttttgcgcga acttcgatgt tatcccagtg
     5041 gcggacgaca gcgagcgtct catcatagtg ctttaaaacg ttacgaatgt tcccggtaag
     5101 gatttttata aagcgcaagc gcacagattg gcttttgatg gtgatttccg ggaacaattt
     5161 aatgataaac ttcatggcgg caatggttcg ttggcaagcc ttaagcgact tgtataggga
     5221 aaaatacagc agcccacacc tgcggctgca tccaggcgcg gaagtatacc actaacatcg
     5281 ctttgctgcg cacatcacct taccattgcg cgttatttgc tatttgccct gagtccgtta
     5341 ccatgacggg gcgaaaaata ttgagagtca gacattcatt atgccgaaga aaaatgaggc
     5401 gcccgccagc tttgaaaagg cgctgagcga gctggaacag attgtaaccc gtctggaaag
     5461 tggcgacctg ccgctggaag aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg
     5521 tcaggggcag gccaaattac aacaagccga acagcgcgta caaattctgc tgtctgacaa
     5581 tgaagacacc tctctacccc cttttacacc ggacaatgag taatggactt tccgcagcaa
     5641 cttgaagcct gcgttaagca ggccaaccag gcgctgagcc gttttatcgc cccactgccc
     5701 tttcagaaca ctcccgtggt cgaaaccatg cagtatggcg cattattagg tggtaagcgc
     5761 ctgcgacctt ttctggttta tgccaccggt catatgttcg gcgttagcac aaacacgctg
     5821 gacgcacccg ctgccgccgt tgagtgtatc cacgcttact cattaattca tgatgattta
     5881 ccggcgatgg atgatgacga tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc
     5941 gaagcaaacg cgattctcgc tggcgacgct ttacaaacgc tggcgttctc gattttaagc
     6001 gatgccgata tgccggaagt gtcggaccgc gacagaattt cgatgatttc agaactggca
     6061 agcgccagcg gtattgccgg aatgtgcggt ggtcaggcat tagatttaga tgcggaaggc
     6121 aaacacgtac ctctggacgc actcgagcgt attcatcgtc ataaaaccgg cgcattgatt
     6181 cgcgccgccg ttcgccttgg tgcattaagc gccggagata aagggcgtcg tgctctgcca
     6241 gtactcgaca agtacgcaga gagcatcggc cttgccttcc aggttcagga tgacatcctg
     6301 gatgtggtgg gagatactgc aacgttggga aaacgccagg gtgccgacca gcaacttggt
     6361 aaaagtacct accctgcact tctgggtctt gagcaagccc ggaagaaagc ccgggatctg
     6421 atcgacgatg cccgccagtc gctgaaacaa ctggctgaac aatcactcga tacctcggca
     6481 ctggaagcgc tagcggacta catcatccag cgtaataaat aaacaataag tattaatagg
     6541 cccctgatga gttttgatat tgccaaatac ccgaccctgg cactggtcga ctccacccag
     6601 gagttacgac tgttgccgaa agagagttta ccgaaactct gcgacgaact gcgccgctat
     6661 ttactcgaca gcgtgagccg ttccagcggg cacttcgcct ccgggctggg cacggtcgaa
     6721 ctgaccgtgg cgctgcacta tgtctacaac accccgtttg accaattgat ttgggatgtg
     6781 gggcatcagg cttatccgca taaaattttg accggacgcc gcgacaaaat cggcaccatc
     6841 cgtcagaaag gcggtctgca tccgttcccg tggcgcggcg aaagcgaata tgacgtatta
     6901 agcgtcgggc attcatcaac ctccatcagt gccggaattg gtattgcggt tactgccgag
     6961 aaagaaggca aaaatcgccg caccgtctgt gtcattggcg atggcgcgat taccgctggc
     7021 atggcgtttg aagcgatgaa tcacgcgggc gatatccgtc ctgatatgct ggtggtcctc
     7081 aacgacaatg aaatgtcgat ttccgaaaat gtcggcgcgc tcaataacca tctggcacag
     7141 ctgctttccg gtaagcttta ctcttcgctg cgcgaaggcg ggaaaaaagt tttctctggc
     7201 gttccgccaa ttaaagagct gctcaaacgt accgaagaac atattaaagg catggtagtg
     7261 cctggcacgt tgtttgaaga gctgggcttt aactacatcg gcccggttga cggtcacgat
     7321 gtgctggggc ttatcaccac gctgaagaac atgcgcgacc tgaaaggccc gcagttcctg
     7381 catatcatga ccaaaaaagg tcgtggttat gaaccggcag aaaaagaccc catcactttc
     7441 cacgccgtgc ctaaatttga tccctccagc ggttgtttgc cgaaaagtag cggcggtttg
     7501 ccgagctatt caaaaatctt tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag
     7561 ctgatggcga ttactccggc gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa
     7621 ttcccggatc gttacttcga cgtggcaatc gccgagcaac acgcggtgac ctttgccgcc
     7681 ggtctggcga ttggtgggta caaacccatt gtcgcgattt actccacttt cctgcaacgc
     7741 gcctatgatc aggtgctgca tgacgtggcg attcaaaagc tcccggtcct gttcgccatc
     7801 gaccgcgcgg gcattgttgg tgctgacggt caaacccatc agggcgcttt tgacctctct
     7861 tacctgcgct gcataccgga aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc
     7921 cagatgctct ataccggcta tcactataac gacggcccgt ccgcggtgcg ctacccgcgc
     7981 ggtaacgcgg ttggcgtgga actgacgccg ctggaaaaac tgccaattgg caaaggcatt
     8041 gtgaagcgtc gtggcgagaa actggcgatc cttaactttg gtacgctgat gccagacgcg
     8101 gcgaaagtcg ctgaatcgct gaacgctacg ctggtcgata tgcgttttgt gaaaccgctt
     8161 gatgaagcgt taattctgga aatggccgcc agccatgaag cgctggtcac cgtagaagaa
     8221 aacgccatta tgggcggcgc aggcagcggc gtgaacgaag tgctaatggc ccatcgtaaa
     8281 ccagtacccg tgctgaacat tggcctgcct gacttcttta ttccacaagg aactcaggaa
     8341 gaaatgcgcg ccgaactcgg cctcgatgcc gccggtatgg aagccaaaat caaggcctgg
     8401 ctggcataat ccctactcca ctcctgctat gcttaagaaa ttattcatgg caggagtggc
     8461 agcatgcaat acaacccctt aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc
     8521 tgtatgacct ttggcgagcc agatcgtggt aatcacgcat ggacactacc ggaagagagt
     8581 agccgtccca tcattaaacg cgcgctggaa ggcggcataa atttctttga taccgccaat
     8641 agctattccg atggcagcag cgaagagatc gttggtcgcg cactgcggga tttcgcccgt
     8701 cgtgaagacg tggtcgttgc gacgaaagtg ttccatcgcg ttggtgattt accggaagga
     8761 ttatcccgtg cacaaatttt gcgctctatc aacgacagcc tgcgccgtct cggcatggat
     8821 tatgtcgata tcctgcaaat tcatcgctgg gattacaaca cgccgatcga agagacgctg
     8881 gaagccctga acgacgtggt aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg
     8941 cacgcttcgc agtttgctca ggccctgaag ctacaaaaac agcacggctg ggcgcagttt
     9001 atcagtatgc aggatcacta caatctgatt tatcgcgaag aagagcgcga gatgctgcct
     9061 ctgtgttatc aggagggcgt ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg
     9121 acgcgtccgt ggggagaaac taccgcacga ctggtgtctg atgaggtggg gaaaaatctc
     9181 tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa
     9241 gaactcggtg caacacgagc acaagttgcg ctggcctggt tattgagtaa accgggcatt
     9301 gccgcaccga ttatcggtac atcgcgggaa gaacagcttg atgagctgct gaacgcggtg
     9361 gatatcactt tgaagccaga acagattgcc gaactggaaa cgccgtataa accgcatgcg
     9421 gtcgtaggat ttaaataaga gtccaggcct gatgagacgt gacaagcgtc acatcaggca
     9481 tcggtgcaca actacgacag aatacccagc ggccagtgat gaccgataaa gtacaggatg
     9541 cctgcggaaa tcaccccggc gacaatatcg tcgatcatga tccccatgcc gccatgtaca
     9601 ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg gaaaatcaca
     9661 aacccggcgg caacccactg ccagtcattg gtcggcagcg ccatgagcgt gatccacata
     9721 ccaataaatt cgtcccagac aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt
     9781 tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga gtagagctgc
     9841 cagggtaaaa aggtcatcag ataccagaac ggaatcgctg ccagcgatcc catcgtgcca
     9901 ggaacgattg ggcttaatcc actcccgaat ccgacagcaa gcagatgcca cggattactc
     9961 atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat atcctttcca
    10021 gtcaaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat
    10081 ttgcccgata caggtaaacg gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg
    10141 gttcagttcc ggcaccgtga aacacaactc gtaatcttca ccgccagaga gcgcccagcg
    10201 cagcgcctgt tccggttcaa catggcgaga aagcgcatca gaaaacggca gcaatgccag
    10261 gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg aaatcagacc
    10321 gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgcccct gtaaaatacg
    10381 cggcgatgga cggagatgac gtttgatcaa gtagtcagca tctttagcat cggcaacctg
    10441 caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac cggtcacata
    10501 gatccagtca cccggtttcg ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc
    10561 gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa tgagttgcat
    10621 atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc acgcttcgtc
    10681 tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca tcgctgccag
    10741 atcgcttagg ttcaccgcca gtgctttata agccagatca gcaggatcga tatcagggag
    10801 gaaatggtta cccgccacca gcgtatcagt gctgatcgcc agggtctgtt tctcggggat
    10861 attgagaagt gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag aacttcttac
    10921 acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt atgcctcagc
    10981 aggtaaaaaa gaaaaggccg acgatcatgg aacggtcttc cgtgaatcta ccggcctgga
    11041 tatcactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc gttgacgaac
    11101 ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt aatggccact
    11161 ttgtatggca catcgctacg tttagacagt tcgtacagcg caatgcgcag tactgctttt
    11221 tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg
    11281 tatgcggtat tagtcgccac cccggccagc agctcacgga agtacaggac gtcaacgtct
    11341 tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt ctgggacaac
    11401 tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc agcaggtttc
    11461 acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa tcatttcaag
    11521 cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac gttcgatcgc
    11581 ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc
    11641 aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaagtggg cagtgccacc
    11701 acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag ccagtgcacc
    11761 cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt tttcatcttt
    11821 tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa
    11881 gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc
    11941 aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg cggattttag
    12001 cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc actaaatgca
    12061 ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg gcgtcggcta
    12121 atttctcaag ccctggcagc gtgcataatc cgcgggcgtc gctgcctaat agtttaggtg
    12181 cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca gcgagcgttg
    12241 gccccgcttc cacccagatg ctgttaattt gctgtttacc cagttgcatc atcagtacaa
    12301 ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt tccggccact
    12361 cactagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat
    12421 gttccggcgt cacgcgattt tggctatcaa tcacaatacg tactggctgg cggagatttt
    12481 gttgcgggta gagcgcctga gtttgttcat ccagttcaga ccaacgcact gttaaggctg
    12541 gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc
    12601 gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg ctcgccatcg
    12661 ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca
    12721 tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc ccgtggctga
    12781 cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc agcgacctgc gggttaggat
    12841 cctgcatcgc tgcgaccacg cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg
    12901 gcgtacggcc atgatggcta cagggttcga gtgtcacata cgccgttgcg cctttggctt
    12961 tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt
    13021 aaccttcacc gacaatttcg ccatctttga caatgacgcc cccgacattc gggttggggt
    13081 gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct
    13141 gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac
    13201 tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc aattgctcca
    13261 tcaccagatt gccaatcatc ttgctcggta cttcgcgctc accggtggcg cgcagctgcg
    13321 atttaatatg attgattgcc atttcaacgt catcggaact caccggacgt ttttccagcg
    13381 cccgcagcat tccgctacgc aatttctctt cattaaacgg ttcacgcacg tcgttgcttt
    13441 ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt tcattacaca
    13501 ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga gagtcaatta
    13561 ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac caggtggtta
    13621 acggaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct tttgagataa
    13681 aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga cgagaaaggg
    13741 acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt tcagccttag
    13801 cgcctgtgtg cagcaaagtg aagtgcgaca gatgaaacac agcgtcagca cgctgaacca
    13861 ggagatgacg caactcaatc aagaaacagt caaaatcact cagcaaaaca ggctgaatgc
    13921 aaaatccagc agtggggttt accttctgcc tggagcgaaa acaccggcaa gactggaaag
    13981 ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatacag atggcaccac
    14041 ccttacacta cgtattcagg gtgagtcgaa tgatcccttg cccgcattca gcggaactgt
    14101 tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg tgcagaatca
    14161 attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc cgttacaatt
    14221 aaaggggatt tctgtggatc agttaggctt tgtacgtatc cacgacattc aacctgttat
    14281 gcagtaaacg tatttcggga cgattttgtg cgtcccgcaa catctttccc cgtcattttg
    14341 ttactctgct tacatcacct ggattgatag taaaagtttg caacaagggc gaaagtcagt
    14401 acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    14461 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    14521 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
    14581 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    14641 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    14701 ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac gatacctacc
    14761 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    14821 cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt tctccgctgt
    14881 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    14941 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    15001 atggtcgcca gagcacctgg tacatgggtc tgggtaccga catcgatact ggtctgccga
    15061 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatgac gcagcgaacg
    15121 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    15181 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    15241 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    15301 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    15361 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    15421 ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga ttatgaaaat
    15481 gccgggattt attcccggca tttctgattg ttaacaacgt gtgctttgtt catgccggat
    15541 gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata
    15601 acgtaggcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    15661 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    15721 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    15781 catgatcgtg gcaatagaga caaagcaatt cccagttact gccatcttcc gggttattgg
    15841 tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttggaataa acaaactcgc
    15901 gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga tagccgcttt
    15961 ctaaccgcgc atagtttttt gggatgatag ccatgaaaag ttacctggat gaaatgtagg
    16021 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    16081 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattacggca gaattgacgg
    16141 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    16201 tttcagagcc aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat
    16261 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat
    16321 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    16381 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaattcggtc
    16441 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    16501 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    16561 cgccagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac
    16621 gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc
    16681 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    16741 aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    16801 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    16861 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    16921 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
    16981 gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    17041 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    17101 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    17161 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    17221 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    17281 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    17341 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    17401 ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg
    17461 gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa
    17521 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    17581 ggcaagcctc cagcccctgc tcaatgttct gcatacccag ggtcgggcca attgtacgtt
    17641 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    17701 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    17761 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    17821 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    17881 tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct
    17941 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc
    18001 gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    18061 cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg
    18121 ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa
    18181 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    18241 cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac
    18301 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg
    18361 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga
    18421 tgctcaggcc gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca
    18481 ggtcactgcg taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    18541 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    18601 catgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    18661 tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga
    18721 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    18781 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    18841 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    18901 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    18961 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    19021 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    19081 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
    19141 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    19201 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
    19261 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    19321 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    19381 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    19441 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    19501 caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    19561 tcaggatcaa gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca
    19621 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    19681 tcgataccac gacgtacgcc ttcaaccagg tcttctggtt taccaacgcc catcaggtaa
    19741 cgcggtttgt ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct
    19801 ttcggctcac ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga
    19861 cctttaacag aaatatcacg taaatcttcg taaacgctgc cctgaatgat accaaacagc
    19921 gcatttttgt ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    19981 atctccatgg agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    20041 atcatgacga tatccgaacc aagatcgtac tgaatctcca ttgatttttc aggatcgagg
    20101 aaaatcggat cgccgttgat cgggttacgg aagtgcacgc cctgttcggt gattttacga
    20161 atatcgccaa ggctgaagac ctggaagccg ccggagtcgg tgaggatcgg ccccttccac
    20221 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    20281 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    20341 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    20401 cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    20461 attttttctc cactacgtca gaaaaacagt ccaacgtttt aaaccagcgc cacggaatta
    20521 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    20581 actaaaaaag cgatatttct cttctaccgc tgctttatag gcgttcatgg tgtgttgata
    20641 accggcaaag gccgaaacca gcataatcag cgtcgattcc ggcaggtgga agtttgtcac
    20701 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcaaa
    20761 gaacggttcg ataagatcgt ttttcgccgc ctgagccgcg ctttccagcg aacgtactga
    20821 ggtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    20881 cacatcctgc ggcacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    20941 gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc
    21001 tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc agacctgcgg tcggggctgc
    21061 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    21121 cggacggtcg atatacggcg gcagcggcat atggccgatg ctgttgagaa tatccagcac
    21181 cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    21241 gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc
    21301 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt tcaaccagca cttcaatctt
    21361 gccgccgctg gctttacgcc caaacaggcg cgccgggatc acgcgggtat tattaaaaac
    21421 cagaagatcg ccggggttga gcttatcaag taaatcggtg aaagtaccgt gcgtcagcgc
    21481 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tgggatagtg
    21541 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    21601 gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    21661 ttttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    21721 aaagctcgct ttccggcaat ttactggctg atttcgtacg cggaaatcct gaagaaagtt
    21781 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta ttaaccgaca
    21841 atctgccgga agtccgcgaa gcacgggaat ggtttcgtag tgaaactcgc cgcgttgcgc
    21901 ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg cagctgtcgc
    21961 cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg acgattttgc
    22021 cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag cagtggctgg
    22081 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgccgcc
    22141 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat gacgccctcg
    22201 aaacccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac aaggcgttat
    22261 aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa tcccgtcagt
    22321 cagttgccca tgcctttctt tgcattgcca ttgcacggca ttgagcaacg gtgacgcggg
    22381 tagcacgact tcacaagctt cgccacggtt gattgccacc agtacacgct gctgattcag
    22441 cacgcggaca aataccacca cgttatcttc cgcatacagc acctgacagc cgccatgacg
    22501 tagcgcctga cttttcttac gcagcgcaat cattcgctgg tacagcgcga ataacgccgt
    22561 atcctgcttt tccacctgcc aggggaacgg tttacggcaa aacggatcgt ttttgccatc
    22621 cagtcctact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    22681 caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc gcgcagtatc
    22741 gtggctgtcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta
    22801 attatccatc caggccatac aggtttgggc atcaatttgc tgcggatcgt aagagatatc
    22861 ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca tggcggcatc
    22921 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga caatatacgc
    22981 ttccggctgg gtttctttcg ccgcttcggt gctcccagca acgtgctgca tattattgcg
    23041 cgccccaccc gcctccccta gcatatgcac cacatccagc cgccagccgt ccatactcca
    23101 cggcgctttc agccaatggc ggacaatact gtcttccccg cgataaattt cattcaccag
    23161 actttccgac tgataatcca gcttcggcaa gctggcatag ccaagccagt cgagcgccgt
    23221 gccatcatca ctaaacgagt accagtcgcg ccagggcgat tcagggttgt gacaagctcc
    23281 ccccgtgcca cgattgtgcc tatcaaacca ggcatgggaa tcgccactgt ggttaaacac
    23341 gccatccagc cccagccgca ttcccagctg ctgcgtattg tggcgtaagc gcagcaacgc
    23401 cccatcaccg ccaaactgcg gatcgacatg gcgataatcc tcggtatcgt atttatgtac
    23461 gctgggagct ttaaacaccg gattgagata cagcgccgtc acgccaagct ttttcagata
    23521 cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc cgcccgcctg
    23581 cgccgtgacc ggttcatccc agtcacgcaa gatgatctct tgtccggctg catgatggta
    23641 atagacatga tcctgttcag cttcacgagg aagactacgc gcaaaacgat cagggaagat
    23701 ctgataaaaa atctgatccg cagcccattg tgggccgata tccggtacat cgacggcaaa
    23761 ctgctccagt cgtgccggcg gcattcggct gaagccctgc ggtgtaaacc aacgctggcg
    23821 atcgtgccac agcagtttga aactgtaacg ccgccggggt tgtccgctgg agagatcaat
    23881 cgccgcacgc catgcggtga cgccaggctg ctgctgactg cgatgcttat gcatcgatac
    23941 tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc
    24001 cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg
    24061 caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc accttgccat
    24121 agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga cgggggcgga
    24181 taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga acgcaaggat
    24241 tactggtttt cagccagctg acgatcgtgg cgacgcttaa acatccagcc aatcaacagc
    24301 acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg ataaccaatc
    24361 aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac cccacccggc
    24421 actttaaatt tcagcgcctt aacttcttct ggcggcaaac agcgacggaa ggcaatttgc
    24481 gacagcagga tcataatcca cacccacacc gtggcgaagg ttgccagcga agcgatcacc
    24541 aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag cgcggtagtc
    24601 atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa aattttcggc
    24661 gcgctgccct gctctgccat accgtggagc atacggccta cgccaaagac gtcactgtta
    24721 attgccgata gcgaagcggt cagcacaaca aagttaagaa tgctagcggc aaaggtaatg
    24781 cccatatgct ggaacgtcag cacgaacggg ctaccggcag tgccaacctg attccacggg
    24841 tagatagaca taatgacgaa cagcgtaccg acgtagaaca ccagaatacg catcggcacg
    24901 gagttaatcg cacgcggtat cgatttctca ggatctttcg cttcaccggc ggtaatcccg
    24961 ataatttcga tcccaccgta agcaaacatc accatttgca acgacatcac catcccaagc
    25021 cagccgttac tgaagaagcc gccgttgctc cacagattat gaataccggt cggttgcccg
    25081 ccgttgccaa tcccccagat gatgatgccg aaaccagcga caatcatgat gatgatggtg
    25141 gcgactttaa agaacgagaa ccagaattcc agctccccga agaccttcac gctcatcagg
    25201 tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg caccgtcggg
    25261 aaccagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac aataaggatt
    25321 tcaaagcagt aggtccagcc ggtaatgtaa cctgccagcg ggccgaggtt ttcctgcgca
    25381 taacgcgaga aagagctggc ggccgggtta tgtaccgaca tttcccccag cgcacgcata
    25441 atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg accggccatt
    25501 ttgatggcgt ctgccgaacc gtaaaacagc ccggtaccaa ttgctgaacc cagtgccata
    25561 aagcgtatgt ggcgggtact tagcccacgc tttagcttgt tcttactttc catcaattcc
    25621 taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt
    25681 tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg
    25741 gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc ggccagcggt
    25801 aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat cccgtcgaga
    25861 ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga attatgccac
    25921 catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat agcggtcagc
    25981 accggtacgg agatctggat cagctggctt aagccgaggt tagaaaccac catcgagaag
    26041 ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc aaagaattct
    26101 gcacaagcac aggtcaggcc aactgccgtt accaagcagg cgatgaagat taacgccgcc
    26161 agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg
    26221 ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacggaa cagcgccagg
    26281 tacagcagag tcagaccaac acccgccatc aggccagccc agacggtata acgggtcagc
    26341 agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa caataacgat accaaacacc
    26401 attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga aaacgcagcg
    26461 ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat cgcggcaaca
    26521 gacaggatga ccagcgcgat aattttcagc ggcgcaagga agttgcccac ggtatccagc
    26581 agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag gctgtagata
    26641 aacagcggca gcgcggaatc accagtcagc ggcgcaatcc ccacttcaaa ggaaacggta
    26701 gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacaaaccgt tgccagcagc
    26761 acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc
    26821 agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc gaatgccgca
    26881 gtccagacgt gttcgccagc ctgcaagccg accattggag ggaaaataat gttacctgcg
    26941 ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga tcttaattga
    27001 tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat
    27061 ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact cccaaccagg
    27121 cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga cagcacctga
    27181 agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa ccagaggtat
    27241 atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa gacatggcta
    27301 atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa
    27361 agaaaataag ccagcatcga ctggcttatg gcaataaaag atgacaaagg cggattaatc
    27421 gctgtttttg gcaattaaac gttccgggat aacaaaactg aaacgtgttc cttttcctac
    27481 tgtactctca atattcaggc gactttcgtg atgattcaca gcatgtttca cgatcgctaa
    27541 ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat aaaaacgctc
    27601 ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc
    27661 ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc gtgccttctg gagtatgatt
    27721 cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac
    27781 tttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca gagtctgagc
    27841 ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga gcaattgcgt
    27901 cggcgcggct tcaattttcg acagcgttag caactgcttc accagccctt ccatccgctg
    27961 ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc
    28021 atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtacgtaact catggctcac
    28081 gttggcaaaa aagttacgcc gcgccccttc cagttgatgc atttgcgtga catcacgcgc
    28141 caccatcagc aactgtttgt gggtataagg catgacgcga atttccagat gccgcccggt
    28201 gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcagatatt gcgtaaactc
    28261 cgggtaacgc agtaggttaa ggatgttctg cccgttatct tccggccagc gcaaaccaag
    28321 aatttgttgc gccagaccgt tacaccagaa aataccgctc tcttccgtgg tcagcaccac
    28381 cgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc ccagttcacg
    28441 gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg gttcccagct
    28501 accacgcccc ggtggcgggg tcatactgcg atccacccac agccaccatg aaaggcgcaa
    28561 taaattccag aaatgccaga taagcagtcc tgttaccgat gccagcaaaa accagggcag
    28621 gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca gctccagcac
    28681 cagccttttc cacgacagcc gttccagcac gtaagatact cccgttaaga aatcataagc
    28741 cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc
    28801 gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac gcaggcgacg
    28861 aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga cgtggtttaa
    28921 cagctgctcg cggctgtaca cgcgctcagg atgcgtcata aaaaagtgca gcagtttaaa
    28981 ttctgtcggc cccatctcca gcggctcttc gcccgccatc actcggtgag atgtcgggtc
    29041 gagacttaat ccctgcatct caatcacctc ttccaccgcc attggcgaaa tacggcgcat
    29101 taccgctttg attcgcgcca ccagctcctt cggcgaaaac ggcttggtga tatagtcatc
    29161 cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac
    29221 cactggaata tcccgggtca tcgactcgcg cttaaggtgt ttgatgaact ggataccgga
    29281 gccgccaggt aacatccagt caaggagaat taaatccggc cagggttcat tcagttgatt
    29341 cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa
    29401 gcagaccatt tcacgaattg gagcttcatc ttctacgacc agaatacgtc tcgccatgat
    29461 ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg cgtcagattt
    29521 atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg ttattgattt ccgtggcgca
    29581 gaaaaaagca aatggcacat ctgtttgggt ataatcgcgc ccatgctttt tcgcctggga
    29641 accgttatgc gcatccttca cacctcagac tggcatctcg gccagaactt ctacagtaaa
    29701 agccgcgaag ctgaacatca ggcttttctt gactggctgc tggagacaac acaaacccat
    29761 caggtggatg cgattattgt tgccggtgat gttttcgata ccggctcgcc gcccagttac
    29821 gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg tcatctggtg
    29881 gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg
    29941 ttcctcaata ctaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc
    30001 gacgggacgc caggcgcagt gctgtgcccc attccgtttt tacgtccgcg tgacattatt
    30061 accagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc agcgattacc
    30121 gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc
    30181 atcatcgcca cgggacattt aacgaccgtg ggggccagta aaagtgacgc tgtgcgtgac
    30241 atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc cgactacatc
    30301 gcgctcgggc atattcaccg cgcacagatt attggcggca tggaacatgt tcgctattgc
    30361 ggctccccca ttccactgag ttttgatgaa tgcggtaaga gtaaatatgt ccatctggtg
    30421 acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt aacgcaaccc
    30481 atggcagtgc tgaaaggcga tctggcgtcg attaccgcac agctggaaca gtggcgcgat
    30541 gtatcgcagg agccaccagt ctggctggat atcgaaatca ctactgatga gtatctgcat
    30601 gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgtcgaagt attgctggta
    30661 cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc
    30721 gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact ggatgaatcg
    30781 cagcagcaac gtctgcagca tcttttcacc acgacgttgc ataccctcgc cggagaacac
    30841 gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga
    30901 agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgctatt accggcccaa
    30961 caggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat cacgaaactc
    31021 cgcgtctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat accgccgaat
    31081 gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta ccgtgcattc tggagccaga
    31141 atcgggcgcg taaccaaccc gacggtaatt tgcaggtgcc acgcgtagag ctggcgcgct
    31201 gcgccgacgg caaaattctc gccgacaaag tgaaagataa gctggaactg acagcgacgt
    31261 taaccgggct ggattacggg cgcttcactc gttcgatgct gctttcgcag gggcaatttg
    31321 ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag ttaaccggca
    31381 ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg gcccgcacag
    31441 agctggagaa gctgcaagcg caggccagcg gcgtcacgtt gctcacgccg gaacaagtgc
    31501 aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta attaccgcgc
    31561 agcagcaaga acaacaatcg ctaaactggt taacgcgtca ggacgaattg cagcaagaag
    31621 ccagccgccg tcagcaggcc ttgcaacagg cgttagccga agaagaaaaa gcgcaacctc
    31681 aactggcggc gcttagtctg gcacaaccgg cacgaaatct tcgtccacac tgggaacgca
    31741 tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca gattgaagaa gtaaatactc
    31801 gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag
    31861 cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac gaccgcttcc
    31921 gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc
    31981 gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa cttaatgcgc
    32041 ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc tagcgccctg gcgcaacatt
    32101 ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt gttccccaac
    32161 aaaaacgtct ggcgcagtta atggtcacta tccagaatgt cactctggaa cagacgcaac
    32221 gtaatgtcgc acttaacgaa atgcgccagc gttataaaga aaagacgcag caacttgccg
    32281 atgtgaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagct caacgtgcac
    32341 agttacaggc gggtcaacct tgtccacttt gtggttccac cagccacccg gcggtcgagg
    32401 cgtatcaggc gctggagcct ggtgttaatc agtctcgatt actggcgctg gaaaacgaag
    32461 ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca actggatgca ttaacgaagc
    32521 agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa gcacttactc
    32581 aacaatggca agccgtcacg accagcctca atatcacctt gcagccacag gacgatattc
    32641 aaccgtggct ggatgcacaa gatgagcacg aacgccagct gcggttactc agccaacgac
    32701 atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat caacagcaaa
    32761 ttgaacaacg ccagcagcag ctcttaacgg cattgacggg ttatgcactg acattgccac
    32821 aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag agctggcagc
    32881 aacgccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgacg ccgattctgg
    32941 aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga aactgtggcg ctggataact
    33001 ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca gcagcagacg ttacagcaac
    33061 aggatgttct ggcggcgcaa agtctgcaaa aagcccaggc gcagtttgac accgcgctac
    33121 aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctaatggat gaacaaacac
    33181 taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc
    33241 tggtcacaca gacagcagaa acgctggcac agcatcaaca acaccgacct gacgggttgg
    33301 ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac caaaagttgc
    33361 gtgaaaacac cacgagtcaa ggcgagattc gccagcagct gaagcaggat gcagataacc
    33421 gtcagcaaca acaaacctta ctgcagcaaa ttgctcaaat gacgcagcag gttgaggact
    33481 ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc aagtttgccc
    33541 aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc cggctgcacg
    33601 ggcgctatct gttacagcgc aaagccagcg aggcgctgga agtcgaggtt gttgatacct
    33661 ggcaggcaga tgcagtacgc gatacccgta ccctttccgg cggcgaaagt ttcctcgtta
    33721 gtctggcgct ggcgctggcg ctttcggatc tggtcagcca taaaacacgt attgactcgc
    33781 tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc gcccttgatg
    33841 cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac gttgaagcga
    33901 tgaaagagcg tattcctgtg cagatcaaag tgaagaagat caacggcctg ggctacagca
    33961 aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat acagaggggc
    34021 gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat tggctacggt
    34081 gattggtagc cgttctggtg gttgtgatgg tggtatgaaa aaagtcattt tatctttggc
    34141 tctgggcacg tttggtttgg ggatggccga atttggcatt atgggcgtgc tcacggagct
    34201 ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt attatgcact
    34261 gggggtggtg gtcggtgcgc caatcatcgc actcttttcc agccgctact cactcaaaca
    34321 tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca cgctctcttc
    34381 gtcttacctg atgcttgcca ttggtcgcct ggtttccggc tttccgcatg gcgcattttt
    34441 tggcgtcggc gcgatcgtgt tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt
    34501 ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc tgggaacgta
    34561 tttaagtcag gaatttagct ggcgttacac ctttttattg atcgctgttt ttaatattgc
    34621 ggtgatggca tcggtctatt tttgggtgcc agatattcgc gacgaggcga aaggaaatct
    34681 gcgcgaacaa tttcactttt tgcgcagccc ggccccgtgg ttaattttcg ccgccacgat
    34741 gtttggcaac gcaggtgtct ttgcctggtt cagctacgta aagccataca tgatgtttat
    34801 ttccgttttt tcggaaacgg cgatgacctt tattatgatg ttagttgggc tagggatggt
    34861 gctgggaaat atgctaagtg gcaggatttc aggacgttat tcaccactgc gcattgcagc
    34921 agtgactgac tttatcattg tactggcact gctgatgctc tttttctgcg gcggcatgaa
    34981 aacaacgtcg cttatttttg cttttatttg ttgcgcggga ttatttgccc tttcagcacc
    35041 tctgcaaata ttgttactgc aaaacgccaa aggcggagag ttattaggtg ccgcaggtgg
    35101 gcaaatagcg tttaacctcg gtagcgccgt cggcgcatat tgcggaggta tgatgctgac
    35161 gctggggctg gcatataatt acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat
    35221 gtcatcgttg ctgctgtatg gtcgctataa gcgccagcaa gcggcggata gtccggtgct
    35281 ggcgaaacca ctggggtaga ttactgtcac ggtgcgcgtt gcttattgcc ggatgcggcg
    35341 tgaacgcctt atccgcccta cgcggttctg gcacattttg taggcctgat aagacgcggc
    35401 aagcgtcgca tcaggcatga tgcgccaatt gcctacgttt tttactcttg tggccataac
    35461 cacgcagcgc cgcgtacgcc gctggaatca ccgtgcttcg ccttacgcac cggcgtttca
    35521 cattcgccgc cgaagacaaa ttgtttaatc aactgcccaa ccgtttgata taaacggtct
    35581 acattgctca tcccgccccc caggacaatc acatccggat cgagaatatt cacgacatgt
    35641 gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc taccggatcg
    35701 ctttcttcaa ccaggcggat aatttcactg cctttcagcg catgtccgct caaacgacga
    35761 taatccgtcg cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa
    35821 taacaaggga cttcctcgcg ataacgcagc tcgtcttcat ccatccacgg tagcggattg
    35881 tgtccccact cgcctgccgt gccattgccg ccgatatgcg cccgcccatt taatgccacg
    35941 cccgcgccgc atcccgtgcc gataatcacg gcaaataccg tctgcgctcc cgctgccgcg
    36001 ccatctactg cttctgaaac cgccagacag ttggcatcat ttgccagccg cacttcccgc
    36061 tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt tgaattggca
    36121 ttcttcacca caccggtgta gggcgaaatc gaacctggaa tgcccatacc taccgttccg
    36181 cgctgccctg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc aatagtctgc
    36241 cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc tgcatcgccc
    36301 agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac tgtactctcc
    36361 ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat gcaaggccgc
    36421 cgacaattcg ttatcatgcc cgctaaattt aacgacaagg ccgtggaaat tatcatgctg
    36481 tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag
    36541 atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga catggcgaag
    36601 atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt tgccaatggt
    36661 caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt gattaaacag
    36721 gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct gaagaaaacc
    36781 gaaaaagatt cgctgaaaga cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt
    36841 tttagccaga caatgatgtg gatcgacacg gttaacggtt tgattatggt ggactgcgcc
    36901 agtgccaaaa aagcggaaga tacgctggca ttactgcgta aaagcctggg gtcgttaccg
    36961 gttgtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc
    37021 ggtagtgcgg cacagggctt ccagctgctt gatgaggccg agctgaaatc gttgctggaa
    37081 gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat caccaatcac
    37141 attgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg cattcagttt
    37201 gtgatgtgcg acgatggttc gctcaagcgt cttaagttct gcgacgagct gcgcgatcaa
    37261 aacgaagata tcgaccgtga agatttcgcc ctgcgttttg atgccgattt catcctgatg
    37321 actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg cgaagcacaa
    37381 cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt
    37441 acgaagccac accaggcata taattattcg ctacggcgag caataatttt tagcgcagca
    37501 atattatgcg ttttacgccg taactgatct aatcgcctca tccctattcg cctgtcagca
    37561 gcacgcagaa tattcactaa cccccattta tcgctggtaa attgttttgt taacagctcc
    37621 gcttttgggg tatcaatata ttcacgcaat aaatcagaaa ttgaacaaat atccgtcagg
    37681 taaggatacc agcaagtttc accgtgaaaa ttacgcactc cagcgttacc tgcaataaag
    37741 tctttcgggt aatcccagat cacgttttta tccaacgtga tccaataacg cggtaaatcg
    37801 gtacttccac cattttggct gcgcatgaga taacgggtac agtgaatttg caggttgatc
    37861 gttggcgtca gtaagtcata gatctcccgc tgtaacttgc tccatggatg ttgtgtcatt
    37921 gtttttcctc aagccggtga cgcgcttcac tgcgaatttt ccgcgttgcc ccggttttca
    37981 ctaaatcacg taactgcgaa agatcgggtt gttcgacaaa taagagtaat gatagatcct
    38041 cttttaacca cgccgttttc acatcggcag ccagctgtgg attattaata gccagcgacc
    38101 gatcctgaga ctcagtcaat gtcgttaaca acgacgccgg ggtatgtgcg ttgcgtaatg
    38161 ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc acgccaccat tcatcatctg
    38221 atttatcttt tatcgtagcc agttccgcaa aaaatacctg gagatcttca ctgatattta
    38281 tatcaacctt gatgaattat gaatatatcg gaaattgaaa aaatcgcagc aaagga
//
LOCUS       DAEEBB010000024        37774 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEEBB010000024 DAEEBB010000000
VERSION     DAEEBB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.024"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="KLF07_001152"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="KLF07_001152"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBD9831226.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="KLF07_001153"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="KLF07_001153"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBD9831227.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="KLF07_001154"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="KLF07_001154"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBD9831228.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="KLF07_001155"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="KLF07_001155"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBD9831229.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="KLF07_001156"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="KLF07_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBD9831230.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="KLF07_001157"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="KLF07_001157"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBD9831231.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="KLF07_001158"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="KLF07_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBD9831232.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="KLF07_001159"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="KLF07_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBD9831233.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="KLF07_001160"
     CDS             6537..6773
                     /locus_tag="KLF07_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBD9831234.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="KLF07_001161"
     CDS             6776..7633
                     /locus_tag="KLF07_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD9831235.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="KLF07_001162"
     CDS             7654..9645
                     /locus_tag="KLF07_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD9831236.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="KLF07_001163"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="KLF07_001163"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBD9831237.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="KLF07_001164"
     CDS             complement(10248..11144)
                     /locus_tag="KLF07_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBD9831238.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="KLF07_001165"
     CDS             complement(11193..11432)
                     /locus_tag="KLF07_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBD9831239.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="KLF07_001166"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="KLF07_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBD9831240.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="KLF07_001167"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="KLF07_001167"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBD9831241.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="KLF07_001168"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="KLF07_001168"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBD9831242.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="KLF07_001169"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="KLF07_001169"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBD9831243.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="KLF07_001170"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="KLF07_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="KLF07_001171"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="KLF07_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBD9831244.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="KLF07_001172"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="KLF07_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBD9831245.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="KLF07_001173"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="KLF07_001173"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBD9831246.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22745)
                     /locus_tag="KLF07_001174"
     CDS             complement(21570..22745)
                     /locus_tag="KLF07_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831247.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22911..23000)
                     /gene="ynhF"
                     /locus_tag="KLF07_001175"
     CDS             complement(22911..23000)
                     /gene="ynhF"
                     /locus_tag="KLF07_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBD9831248.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23299..24324
                     /gene="purR"
                     /locus_tag="KLF07_001176"
     CDS             23299..24324
                     /gene="purR"
                     /locus_tag="KLF07_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBD9831249.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24321..25253)
                     /locus_tag="KLF07_001177"
     CDS             complement(24321..25253)
                     /locus_tag="KLF07_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9831250.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25366..26577
                     /locus_tag="KLF07_001178"
     CDS             25366..26577
                     /locus_tag="KLF07_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBD9831251.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26868..28016
                     /gene="cfa"
                     /locus_tag="KLF07_001179"
     CDS             26868..28016
                     /gene="cfa"
                     /locus_tag="KLF07_001179"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBD9831252.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28056..28697)
                     /gene="ribE"
                     /locus_tag="KLF07_001180"
     CDS             complement(28056..28697)
                     /gene="ribE"
                     /locus_tag="KLF07_001180"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBD9831253.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28912..30285
                     /gene="mdtK"
                     /locus_tag="KLF07_001181"
     CDS             28912..30285
                     /gene="mdtK"
                     /locus_tag="KLF07_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBD9831254.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30326..31582)
                     /locus_tag="KLF07_001182"
     CDS             complement(30326..31582)
                     /locus_tag="KLF07_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBD9831255.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31890..31966
                     /locus_tag="KLF07_001183"
     tRNA            31890..31966
                     /locus_tag="KLF07_001183"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31924..31926,aa:Val,seq:gac)
     gene            31971..32047
                     /locus_tag="KLF07_001184"
     tRNA            31971..32047
                     /locus_tag="KLF07_001184"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32005..32007,aa:Val,seq:gac)
     gene            32155..32460
                     /locus_tag="KLF07_001185"
     CDS             32155..32460
                     /locus_tag="KLF07_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBD9831256.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32586..34190
                     /locus_tag="KLF07_001186"
     CDS             32586..34190
                     /locus_tag="KLF07_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBD9831257.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34202..35014)
                     /locus_tag="KLF07_001187"
     CDS             complement(34202..35014)
                     /locus_tag="KLF07_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831258.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35018..35803)
                     /locus_tag="KLF07_001188"
     CDS             complement(35018..35803)
                     /locus_tag="KLF07_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBD9831259.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35800..36468)
                     /locus_tag="KLF07_001189"
     CDS             complement(35800..36468)
                     /locus_tag="KLF07_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBD9831260.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36532..37170)
                     /locus_tag="KLF07_001190"
     CDS             complement(36532..37170)
                     /locus_tag="KLF07_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBD9831261.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37183..>37774)
                     /locus_tag="KLF07_001191"
     CDS             complement(37183..>37774)
                     /locus_tag="KLF07_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBD9831262.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9024 a         9933 c         9382 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagcg ccagcaacgg atagttaatt
    22741 ttcatgcctt atctccacct cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc
    22801 atgacagcaa tcacaaaaaa atgaaaataa caaaaagaga aaacactttt gccattttgc
    22861 taacaaacag gaaggagatg cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc
    22921 gcggcagtca aacccacggc tacgatcaaa ccgaggacga taatcgttgt taccagtgaa
    22981 aatttaaggt cggtgctcat caagttttct ccttttttat taccacacaa aaagtgatat
    23041 tacgcatttt tacacactgt gatgaaaaaa tctcccgtca tttataatga taagtgtttt
    23101 taccacttcc ccttttcgtc aagatcggcc aaaattccac gcttacacta tttgcgtact
    23161 ggccattgac cccttcctga cgctccgtgt cgtttttccg gcgtaccgca acacttttgt
    23221 tgtgcgtaag gtgtgtaaag gcaaacgttt accttgcgat tttgcaggag ctgaagttag
    23281 ggtctggagt gaaatggaat ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc
    23341 actacaactg tgtcacacgt gatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac
    23401 gccgtgtggg cagcgattaa agaattacac tactccccta gcgcggtggc gcgtagcctg
    23461 aaggttaacc acaccaagtc tatcggtttg ctggcgacca gcagcgaagc ggcctatttt
    23521 gccgagatca ttgaagcagt tgaaaaaaat tgcttccaga aaggttatac cctgattctg
    23581 ggcaatgcgt ggaacaatct tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa
    23641 cgcgtcgatg gtctgctggt gatgtgttct gagtacccag agccgttgct ggcgatgctg
    23701 gaagagtatc gccatatccc aatggtggtg atggactggg gtgaagcaaa agctgacttc
    23761 accgatgcgg tcattgataa cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt
    23821 gaacgcggtc accgcgaaat cggcgttatc cccggcccgc tggaacgtaa cactggcgca
    23881 ggccgccttg tcggttttat gaaggcgatg gaagaagcaa tgatcaaggt gccggaaagc
    23941 tggattgtgc agggtgactt tgaacctgaa tccggttatc gcgccatgca gcaaatcctg
    24001 tcgcagccgc atcgccctac tgccgtcttc tgtggtggcg atatcatggc aatgggcgca
    24061 ctttgtgctg ctgatgaaat ggggctgcgc gtcccgcagg atgtttcgct aattggttat
    24121 gataacgtgc gcaacgcccg ctattttacg ccggcgctga ccacaatcca tcagccaaaa
    24181 gattcgctgg gtgaaacagc gttcaacatg ctgttggatc gtatcgtcaa caaacgtgaa
    24241 gaaccgcagt ccattgaagt gcatccgcgc ttgattgaac gccgctccgt ggctgacggc
    24301 ccgttccgcg actatcgtcg ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc
    24361 cactccttat tcagcgtctc actatcgccg agatactcaa gcaaccaggt taacgcaggc
    24421 gacatatcat tttgctgcca tgtcagacaa catgccgaat ccggaaaggg gttttccagt
    24481 tcaagtgcta cccacttccc ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc
    24541 cctatgcata atcctgccga gatacaggtt gccgatgatt cccagtcagg cacgacaact
    24601 cttttttggt tatccagcaa ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg
    24661 cgcaccaacg acggccagtt gcgcaacgta tcatcgctga acgggccatc catcaacgcc
    24721 agcgggtgat ggctggcaac aacgcaactc cagcttagca tccccatatc ccggaaggca
    24781 taacgaccgc ctaccggaat cgcgcgtgtt gcgccaatcg ccagttccac gcgcccgtcg
    24841 gaaagcgcat cccagacacc gttgaacact tcctgaaaga caagaagttc gacatcatca
    24901 aaatggcgat aaaaatcaac gatcatctgc cgtgtacgtt ctggcctgac aatattatcc
    24961 actgcgatag ctaactgacc gcgccagccg ttcgctatct gctgacattg ctggcgggtg
    25021 atctgcattt ttttgacaac agagcgccct tctttgagaa accacgctcc agcagcggtc
    25081 agctccacat cacggtgccg tcgttcaaag agcggcaccg ccagccactc ttccagctga
    25141 cgcacggtat agctgaccgc agaaggaacg cgatgcagct cctgtgccgc agcgctaaaa
    25201 ctaccattac gcgctaccgc atcaacaact tcgagtgaat attctgacca catagtctgc
    25261 ctgcaaaatt tttgaaacca atcatcaaat attaccgttt cacaacacta atttcactcc
    25321 ctacactttg cggcggtgtt taattgagag atttagagaa tatacatgca acctgggaaa
    25381 agatttttag tctggctggc gggtttgagc gtactcggtt ttctggcaac cgatatgtat
    25441 ctgcctgctt tcgccgccat acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc
    25501 agccttagtc tgttccttgc cggttttgcc gcagcccagc ttctgtgggg gccgctctcc
    25561 gaccgttatg gtcgtaaacc ggtattatta atcggtatga caatttttgc gttaggtagt
    25621 ctggggatgc tgtgggtaga aaacgccgct acgctgctgg tattgcgttt tgtacaggct
    25681 gtgggtgtct gcgccgcggc ggttatctgg caagcgttag tgacggatta ttatccttca
    25741 cagaaagtta accgaatttt tgcgaccatc ataccgctgg tgggtctatc tccggccctg
    25801 gctcctctgt taggaagctg gctgctggtc catttttcct ggcaggcgat tttcgccacc
    25861 ctgtttgcca ttaccgtggt gctgattctg cctattttct ggctcaaacc cacgacgaag
    25921 gcccgtaaca atagtcagga tggtctgacc tttaccgacc tgctacgttc taaaacctat
    25981 cgcggcaacg tgctgatata cgcggcctgt tcagccagtt tttttgcatg gctgaccggt
    26041 tcaccgttca tccttagtga aatgggctac agcccggcag ttattggttt aagttatgtc
    26101 ccgcaaacta tcgcgtttct gattggtggt tatggctgtc gcgccgcgct gcagaaatgg
    26161 caaggcaagc agttattacc gtggttgctg gtgctgtttg ctgtcagcgt cattgcgacc
    26221 tgggctgcgg gcttcattag ccatgtgtcg ctggtcgaaa tcctgatccc attctgtgtg
    26281 atggcgattg ccaatggcgc gatctaccct attgttgtcg cccaggcgct gcgtcccttc
    26341 ccacacgcaa ctggtcgcgc cgcagcgttg cagaacactc tacaactggg tctgtgcttc
    26401 ctcgcaagtc tggtagtttc ctggcttatt agtatcagca cgccattgct caccaccacc
    26461 agcgtgatgt tatcaacagt agtgctggtc gcgctgggtt acatgatgca acgttgtgaa
    26521 gaagctggct gccagaatca tggcaatgcc gaagtcgctc atagcgaatc acactgatct
    26581 atatcgatat acttatactt aggctgctaa caaaattttg ttgtatgatt gaaattagcg
    26641 gcctatacta atttcgagtt gttaaagcta cgataaatat tatgttttta cggggacagg
    26701 atcgttcccg actcactatg gatggtcatt tcggcaaggg ttcctccttt ccctctgttc
    26761 tacgtcggat tatagactcg cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg
    26821 tctacgctgt tttaaggttc tgatcaccga ccagtgatgg agaaactatg agttcatcgt
    26881 gtatagaaga agtcagtgta ccggatgaca actggtaccg tatcgccaac gaattactta
    26941 gccgtgccgg tatagccatt aacggttctg ccccggcgga tattcgtgtg aaaaaccccg
    27001 atttttttaa acgcgttctg caagaaggct ctttggggtt aggcgaaagt tatatggatg
    27061 gctggtggga atgtgaccga ctggatatgt tttttagcaa agtcttacgc gcaggtctcg
    27121 agaaccaact cccccatcat ttcaaagaca cgctgcgtat tgccggcgct cgtctcttca
    27181 atctgcagag taaaaaacgt gcctggatag tcggcaagga gcattacgat ttgggtaatg
    27241 acttgttcag ccgcatgctt gatcccttca tgcaatattc ctgcgcttac tggaaagatg
    27301 ccgataatct ggaatctgcc cagcaggcga agctcaaaat gatttgtgaa aaattgcagt
    27361 taaaacctgg gatgcgcgta ctggatattg gctgcggctg gggcggactg gcacactaca
    27421 tggcatctaa ttatgacgta agcgtggtgg gcgtcaccat ttctgccgaa cagcaaaaaa
    27481 tggctcagga acgctgtgaa ggcctggatg tcaccatttt gctgcaagat tatcgtgacc
    27541 tgaacgacca gtttgatcgt attgtttctg tggggatgtt cgagcacgtc ggaccgaaaa
    27601 attacgatac ctattttgcg gtggtggatc gtaatttgaa accggaaggc atattcctgc
    27661 tccatactat cggttcgaaa aaaaccgatc tgaatgttga tccctggatt aataaatata
    27721 tttttccgaa cggttgcctg ccctctgtac gccagattgc tcagtccagc gaaccccact
    27781 ttgtgatgga agactggcat aacttcggtg ctgattacga tactacgttg atggcgtggt
    27841 atgaacgatt cctcgccgca tggccagaaa ttgcggataa ctacagtgaa cgctttaaac
    27901 ggatgtttac ctattatctg aatgcctgcg caggtgcttt ccgcgcccgt gatattcagc
    27961 tctggcaggt cgtgttctca cgcggtgttg aaaacggcct tcgagtcgct cgctaaaggc
    28021 tattctatcg ccccctctcc gggggcgatt tcagatcagg cttctgtacc tggttgattc
    28081 atggcatttt ctcgtgccgc cagcacacgt tctaccgtat ctaccactgc ctgagtttgt
    28141 ggatcaattt caatgttgac gcgtgcgccg agttttttct tcccaagagt cgtgcgttcc
    28201 agagtttccg gaattaaatg gacgcaaaaa cgcgttggcg tgacttcgcc gacggtcagg
    28261 ctaataccgt cgatgccaat aaatcctttg tacaggatat atttcatcaa ctgactatcc
    28321 tggactttaa accagatctg gcgattattt tccgaggtta atattttcgc cacttcagcg
    28381 gtggtcataa tatgacctga cattaagtgt ccgccaattt catcgctgaa ttttgccgca
    28441 cgctcaacgt ttacccaatc ccccactttt aaatcgccaa gattggtaat gcgtaacgtt
    28501 tctttcatca ggtcaaaact gacatggttg ccgttaattt ccgtcactgt caggcagcaa
    28561 ccgttatgcg ccacggaagc acctgtttcc aggccgtcca gcatgtggtc gggtaactcc
    28621 accacatgcg tacgaaaatt tggtttctcg tcaatcgaca ccagttttgc ggtgccctgt
    28681 acaatccccg taaacatact tacaactcct gaaatcagtt aagacattct gttcagcaca
    28741 atagcaggtg gaaaacgccc ttaccagtga aggggtaaga atgactattt tttcactgga
    28801 gaattaataa atcctcgcta caatagactg aatttcccct gcttcttctt tttgctgccc
    28861 attcaggcgg ctttttagtc tctcatataa ctacaaataa aaggtgttca cgtgcagaag
    28921 tatatcagtg aagcgcgtct gttattagca ttagcaatcc cggtgattct cgcgcaaatc
    28981 gcccaaactg cgatgggttt tgtcgatacc gtgatggcgg gcggctatag tgccaccgac
    29041 atggcggcgg tcgctatcgg tacttctatc tggcttccgg cgatcctctt tggtcacgga
    29101 ctgctgctgg cattaacgcc ggttatcgcg caattaaatg gttccggtcg acgtgagcgc
    29161 attgcgcatc aggtgcgaca aggtttctgg ctggcaggtt ttgtttccgt tctcattatg
    29221 ctggtgctgt ggaatgcagg ttacattatc cgctccatgg aaaacatcga tccggctctg
    29281 gcggacaaag ccgtgggtta tctgcgtgcg ttgttgtggg gcgcgccagg ctatcttttc
    29341 ttccaggttg cccgtaacca gtgtgaaggt ctggcaaaaa ccaagccggg tatggtaatg
    29401 ggctttatcg gcctgctggt gaacattccg gtgaactata tctttattta tggtcatttc
    29461 ggtatgcctg agctcggtgg cgttggttgt ggcgtggcta ctgcggcggt gtattgggtt
    29521 atgttccttg ccatggtttc ttacattaaa cgcgcccgct ccatgcgcga tatccgtaac
    29581 gaaaaaggca ccgcaaaacc cgatcctgcg gttatgaaac gactgattca actcggtttg
    29641 ccgattgcgc tggcactatt ctttgaagtg acactgtttg ccgtcgtggc tctgttagtg
    29701 tctccgctcg gtattgttga tgtcgcagga caccagattg ccctgaactt tagttcacta
    29761 atgttcgtgc ttccaatgtc gctggcggca gcggtaacta tccgcgtagg ttatcgtctg
    29821 ggtcagggct caacgctgga tgcgcaaacc gctgctcgga ccgggcttat ggtgggtgtc
    29881 tgtatggcaa ccctgacggc cattttcacg gtttcactgc gggagcaaat cgccctgttg
    29941 tacaacgaca atcccgaggt tgtaacgctg gctgcgcatt tgatgttgct ggcggcggta
    30001 tatcagattt ctgactcaat ccaggtgatt ggcagtggga ttttgcgtgg ttataaagat
    30061 acgcgttcca ttttctatat cacctttaca gcttactggg tgctgggctt gccaagcggc
    30121 tatattctgg cactgaccga tctggtcgtt gaacctatgg ggccagcagg cttctggata
    30181 ggctttatta tcggcctgac atcggcagcc attatgatga tgctgcgtat gcggttcctg
    30241 caacgtatgc cgtcagccat cattctgcaa cgagcgtccc gctaataaag acaaggcgca
    30301 accttcacgg gttgcgcctg tgtttttacg caggctggag cgttgcgcca atcccgtctt
    30361 cgtctggctg taatttcaga gcgttacaca gagtttgccg actggcatct gttatctcaa
    30421 cgcgtatatc cccgttaact tcccctttca cattgatgaa gtcaggtgaa atttctttcg
    30481 cactggtttg caggcaaatc gctccctctc cgcgcaggcg tttcactttc agttgcacat
    30541 gcgttgccgc accagtgatc agccacagtt ttccgccatt cagattgatg ttaccaacaa
    30601 tgaaagtgac gctgtcacct tttaccgtac tgccgtcgag aatcaacaca ccgccctgct
    30661 gtacagtaat cgttgattgt tgcagctctg cgccataacc aataatcatt gaagcgccgt
    30721 ccagtaatgc aacactcccg gtcagttcaa ccggtgcgcg atcttcctct acgacaaaat
    30781 tttcgtccgc gctggtgggt gacaacagga tcactgttcc accttgtatt accgcatttt
    30841 ccagcgtacc ggcatgcact tcagcccgcc cccccacatt caccgacagg ttttgcgtct
    30901 taccggaact gtagagctga aggccatccg tccccagacg ataaatggat ccattctcaa
    30961 caatagtgtc agagtcttca ccaccgagat tattcatggt gccaccagca agcaacgtat
    31021 cactggcttt cccggccagc ataaaaacgc cgccctgatt aatctcacaa ttgctggctt
    31081 ctccacttgg atacaccaac aattcaccac cttcatctac cacgaccgct gaggttgtcc
    31141 cattaaccaa caggccgccc tcttgatcga gaatgatttt ttccgcgcga tgtccttcca
    31201 gtacacgcag gttaccgcca ttttccagca acaaaccgca ggcataaccc tgatcaacgc
    31261 tgaattcacc ttcgggatgg cggccattaa ccgtagcgag cgtagagaga ctaattgcgc
    31321 ctccctcgga ctgaacaata tctgtggcaa tggcaccgtc gctgaccgat agcgtgccat
    31381 tgttaccgta ctgtgtgcat ttggcgatcc caccggttgc cactttcaac cagccagccc
    31441 ccgtgaccac ggtattttcg gctcttccgc cagcttcaac aatcaactcg ccttcagtaa
    31501 gttgcgtcgc gccaatgacc tcgccggtct taacaatttg cacattcccg tcgctcatca
    31561 agtttttcgc atcagatccc atgatttatt cctttgctgc atctgtgtgc ctttattgct
    31621 acctaagtgt aaaggctacg gaggatttat ccacgacaga tttgagatgg tggcaaacaa
    31681 ttctgtttaa actctgatac acgaattatt gggttgtatc agatgtaaat gcgatcctga
    31741 ataaaaatcg cccttgcaaa tcaacaaaat atagccaatt tgcttaaaag ctctgcaaac
    31801 ggtcgaaata gcgcagaaaa ttacgttttg cctcttgcca cctttccact ctgccgctaa
    31861 tatttgtccc cgttgtcacc tacaacgttg cgttcatagc tcagttggtt agagcaccac
    31921 cttgacatgg tgggggtcgt tggttcgagt ccaattgaac gcaccatcct gcgtccgtag
    31981 ctcagttggt tagagcacca ccttgacatg gtgggggtcg gtggttcgag tccactcgga
    32041 cgcaccagat tttcttaatc tggtcttttc ctttttccct ctgtttattc tctgtatcca
    32101 atacgttaaa agatttacac tgtcttcgta tgcgttatca gaaggagaat cgctatggca
    32161 actttgttac aacttcattt tgcttttaat ggcccatttg gcgacgcaat ggctgagcag
    32221 cttaaaccac ttgctgagtc gattaatcag gaacctggtt ttctgtggaa ggtatggaca
    32281 gaaagtgaaa agaaccacga agccggtggg atctaccttt tcactgatga aaaaagcgcc
    32341 cttgcctatc tggaaaaaca tactgcccga ctgaaaaatc tcggcgttga ggaagttgtc
    32401 gccaaagttt tcgatgtcaa tgagccactt agtcaaatca atcaggcaaa actcgcctga
    32461 cagaatttaa tcaagggcgg ttagcgccct tttcatccct gtctgaaatt tctcaaattc
    32521 taaaaatctc aaccaaactt atctgataac actaaattcg aaagaatgcg tacaggtaag
    32581 taacaatgaa aaaaattgct attgtgggtg ccgggcctac ggggatctac accttattct
    32641 cgcttctaca gcaacaaact ccactttcta tttctatctt cgagcaggct gacgaggccg
    32701 gtgtcgggat gccatacagt gatgaggaaa actcaaaaat gatgctggca aatattgcca
    32761 gtattgaaat accgccgatt aattgtacgt atctcgaatg gctacaaaag caagaagcca
    32821 gccatctcca gcgttatggc gttaaaaaag aaaccttgca cgatcgtcag tttttaccgc
    32881 gaattctgct gggcgaatat ttccgcgatc aatttttacg attagtagac caggcacgac
    32941 agcaaaaatt tgcagtggct gtttatgaat catgccaggt taccgatctg caaattacaa
    33001 atgctggcgt catgctcgct acaaatcagg atttacccag cgagacgttt gatttagcgg
    33061 tgattgccac gggtcacgtc tggcctgatg aagaagaagc aaccagaacg tattttccca
    33121 gcccgtggtc aggcctgatg gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa
    33181 catccttgag cggactggat gcggcaatgg cagtggctat tcagcatggt tcgttcattg
    33241 aagatgataa acaacacgtc gtttttcacc gcgataacgc aagtgaaaag ctaaatatca
    33301 cgttgatgtc gcgcacgggt attttacccg aagccgattt ctattgccct attccctacg
    33361 agcccttaca catcgtcacc gatcaggcat taaatgctga gattcaaaaa ggcgaatatg
    33421 gccttttgga tcgggtattt agattgatag tagaggaaat caagtttgct gatccagact
    33481 ggagccaacg catagcctta gagagcctga atgtcgattc ctttgctcaa gcctggtttg
    33541 ccgagcgcaa acaacgcgac ccatttgact gggcagaaaa aaatctccag gaagtcgaac
    33601 gcaataaacg agaaaaacat actgttccct ggcgttatgt cattctgcgc ctgcatgaag
    33661 ccgtacagga aattgttcca catctgaatg aacacgacca taaacggttc agtaaaggcc
    33721 ttgcccgggt tttcatcgat aattatgcgg caatcccttc agagtctatt cgtcggctgc
    33781 tggccttacg tgaagcgggg atcattcata ttctcgccct cggtgaagac tacaaaatgg
    33841 aaattaatga gtcgcgcacc gtcctgaaaa cggaagacaa cagctactcg tttgacgttt
    33901 ttattgatgc ccgcggacag cgtccgctta aagtgaaaga tattcctttc cctgggctac
    33961 gcgagcaatt acagaaaaca ggggatgaaa tccctgatgt tggcgaagat tatacgttac
    34021 agcaacccga agatattcgt ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg
    34081 accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg atggctcggg
    34141 cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct
    34201 atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg gttcgaatat
    34261 tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga tatgccagtg
    34321 cggcgcttcc ccctggcccg agaataattg agtcgaccac gcccgtgctc actaccgttg
    34381 aattttcacc gctggcaatg atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg
    34441 cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac cgttcaccca
    34501 atgtacaaac gcgtactcgc attccggtat tcgcaatccg actactatct ccagcgctgc
    34561 ttatccgcga agaattaccg gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt
    34621 gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca atttgcgcgg
    34681 catacccagc ggttacgatt cgggcgttat cctcacaaca gccgatgcga gcatgatcgt
    34741 cgctgcgggc aaactgactg caaaatggta tctctccggg gtctgtggcc agtttttgca
    34801 tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc cacgcatact
    34861 caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga tgaatatcgg
    34921 cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc gctccgatac
    34981 gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga tagccatcga
    35041 ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa agatgtccga
    35101 agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac cagtatctta
    35161 cgccaggaaa cacatctccc acggcttgcg gatagagaca caacagcccc gtcaatagta
    35221 acaacggcag caatccacac atgacaccaa cataggcgac ctgctgtaag ggattaaatt
    35281 tagactgtgt tgttgccggg aaaggatgtt cttccccctg cataatgcca aacaaataaa
    35341 atcgtgtttg ttttgccgct cgttccagcc acccctgacg acgaatgcga tagtggtgac
    35401 cattatcccc aacggcattg atcagcacaa agccgagcca gcatgccagt aacaaaaatc
    35461 cgcaaacttc atgcaccgca accagacttt taaccacagt tgcgcccacc atcgcaaagt
    35521 gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac cagtgccata
    35581 atctgaccgc tttgctgtaa agataaacct tctcaccatg atcggtggct gatttcttca
    35641 cgccacgagc acgaagcaag gcgtgcagcc ccaacaccaa ccacatccca accagcaata
    35701 ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc acatagttcg
    35761 ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt cctttttaat
    35821 caaatgttga ccgaaccggc gatacagatg cggtttcccg gcaccaagta gctgatattg
    35881 atagtattta ttttcctgca accacgcctg aatttctggg ctatcttcgc gaccaaagat
    35941 caatgcgtgt tccggacagg cgctgacgca aatgggcgga aagccttttg ccagtcggga
    36001 ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt agcgaacctg
    36061 gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt ctacccgcac
    36121 gatcccctgc tcatcacgcc acgacgcccc tgtcgggcat acgtcgatgc acggagcatc
    36181 ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt
    36241 gactggaatg tgtgctatcg ataaacggct tccctgagcg gggacgtggt tagttttacg
    36301 gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa tcatggcata
    36361 ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa
    36421 gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct gattatcccc
    36481 taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat ttcaggggtt
    36541 ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat aggtcataaa
    36601 tcgctcgcta aacaagtaca acaccgccgt cgatgactcc acctgccgta tatcggcata
    36661 ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct ggaagtaata
    36721 tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc actcaaaacg
    36781 aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag acatcgccgc
    36841 gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc ccgtcataca
    36901 agcgatatcc gcgtagtctc cgttctgctt aatgctttcc agcaaaactg acagttcctc
    36961 ttctgcaacc gaatagggtg gcgagagaaa cacggtgcgc gccaccagct gcccagccgc
    37021 agaatgctgg cgaatgaact cagccaggat ttcagcaggt tccggttcgg ttaccgtatc
    37081 aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt
    37141 cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag gcagtagatt
    37201 gtgtgctgcc agatcggcgg caatatcttc cagccccagc cgctgcaatg tttcgcgggt
    37261 tggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga gcgaagcatg
    37321 catatcgtca cgatccattt tgtcagtacc ttcagtaaag accgggatct gcggatcctt
    37381 gtcgaaaacc caggaacaga taagatcgtg ttcattacgc atatctttgg tttgcatcag
    37441 ttttaccgtg taggcacgat gcaacgtaaa gatacgctct gcagctaaat ctaatttttc
    37501 ctgagtcatc tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc
    37561 ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt tgcacagggt
    37621 gacggcgtta tgcaaacaga cccgcaacag cgaccacttt gcatatttaa tttttgcgtc
    37681 attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa aaagttcttt
    37741 cgccacttca cgttgcagtt tcaatggcaa gccg
//
LOCUS       DAEEBB010000025        37715 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEEBB010000025 DAEEBB010000000
VERSION     DAEEBB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.025"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..160
                     /locus_tag="KLF07_001192"
     CDS             <1..160
                     /locus_tag="KLF07_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBD9831263.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="KLF07_001193"
     CDS             complement(198..1259)
                     /locus_tag="KLF07_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD9831264.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="KLF07_001194"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="KLF07_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBD9831265.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="KLF07_001195"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="KLF07_001195"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBD9831266.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="KLF07_001196"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="KLF07_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBD9831267.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="KLF07_001197"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="KLF07_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBD9831268.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="KLF07_001198"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="KLF07_001198"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBD9831269.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="KLF07_001199"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="KLF07_001199"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBD9831270.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="KLF07_001200"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="KLF07_001200"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBD9831271.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="KLF07_001201"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="KLF07_001201"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBD9831272.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="KLF07_001202"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="KLF07_001202"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBD9831273.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="KLF07_001203"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="KLF07_001203"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD9831274.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="KLF07_001204"
     CDS             complement(14497..14649)
                     /locus_tag="KLF07_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831275.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="KLF07_001205"
     CDS             14667..14858
                     /locus_tag="KLF07_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBD9831276.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="KLF07_001206"
     CDS             15169..15687
                     /locus_tag="KLF07_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD9831277.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="KLF07_001207"
     CDS             15703..16242
                     /locus_tag="KLF07_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBD9831278.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="KLF07_001208"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="KLF07_001208"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBD9831279.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="KLF07_001209"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="KLF07_001209"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBD9831280.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="KLF07_001210"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="KLF07_001210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBD9831281.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="KLF07_001211"
     CDS             complement(20978..21193)
                     /locus_tag="KLF07_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBD9831282.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="KLF07_001212"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="KLF07_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBD9831283.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="KLF07_001213"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="KLF07_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBD9831284.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="KLF07_001214"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="KLF07_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBD9831285.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="KLF07_001215"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="KLF07_001215"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBD9831286.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="KLF07_001216"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="KLF07_001216"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBD9831287.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="KLF07_001217"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="KLF07_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBD9831288.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="KLF07_001218"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="KLF07_001218"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBD9831289.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="KLF07_001219"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="KLF07_001219"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBD9831290.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="KLF07_001220"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="KLF07_001220"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBD9831291.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="KLF07_001221"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="KLF07_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBD9831292.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
BASE COUNT         9393 a        10158 c         9311 g         8853 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga caattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatg
//
LOCUS       DAEEBB010000026        37264 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEEBB010000026 DAEEBB010000000
VERSION     DAEEBB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.026"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..642)
                     /locus_tag="KLF07_001222"
     CDS             complement(<1..642)
                     /locus_tag="KLF07_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HBD9831293.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="KLF07_001223"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="KLF07_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBD9831294.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="KLF07_001224"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="KLF07_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBD9831295.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="KLF07_001225"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="KLF07_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBD9831296.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="KLF07_001226"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="KLF07_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBD9831297.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="KLF07_001227"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="KLF07_001227"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD9831298.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="KLF07_001228"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="KLF07_001228"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBD9831299.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="KLF07_001229"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="KLF07_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBD9831300.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="KLF07_001230"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="KLF07_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBD9831301.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="KLF07_001231"
     CDS             10135..11034
                     /locus_tag="KLF07_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBD9831302.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="KLF07_001232"
     CDS             11040..13472
                     /locus_tag="KLF07_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBD9831303.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="KLF07_001233"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="KLF07_001233"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBD9831304.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="KLF07_001234"
     CDS             14585..15850
                     /locus_tag="KLF07_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBD9831305.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="KLF07_001235"
     CDS             complement(15817..16977)
                     /locus_tag="KLF07_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD9831306.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="KLF07_001236"
     CDS             17215..18516
                     /locus_tag="KLF07_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831307.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="KLF07_001237"
     CDS             18531..20894
                     /locus_tag="KLF07_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBD9831308.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="KLF07_001238"
     CDS             complement(21074..22669)
                     /locus_tag="KLF07_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBD9831309.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="KLF07_001239"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="KLF07_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD9831310.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="KLF07_001240"
     CDS             complement(23867..24985)
                     /locus_tag="KLF07_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBD9831311.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="KLF07_001241"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="KLF07_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBD9831312.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="KLF07_001242"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="KLF07_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBD9831313.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="KLF07_001243"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="KLF07_001243"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD9831314.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="KLF07_001244"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="KLF07_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBD9831315.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="KLF07_001245"
     CDS             complement(28235..28300)
                     /locus_tag="KLF07_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831316.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28542..29429
                     /gene="rhtA"
                     /locus_tag="KLF07_001246"
     CDS             28542..29429
                     /gene="rhtA"
                     /locus_tag="KLF07_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBD9831317.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29728..30231
                     /gene="dps"
                     /locus_tag="KLF07_001247"
                     /gene_synonym="pexB"
     CDS             29728..30231
                     /gene="dps"
                     /locus_tag="KLF07_001247"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBD9831318.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30635..31381
                     /gene="glnH"
                     /locus_tag="KLF07_001248"
     CDS             30635..31381
                     /gene="glnH"
                     /locus_tag="KLF07_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBD9831319.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31520..32179
                     /gene="glnP"
                     /locus_tag="KLF07_001249"
     CDS             31520..32179
                     /gene="glnP"
                     /locus_tag="KLF07_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBD9831320.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32176..32898
                     /gene="glnQ"
                     /locus_tag="KLF07_001250"
     CDS             32176..32898
                     /gene="glnQ"
                     /locus_tag="KLF07_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBD9831321.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33015..35240
                     /gene="ybiO"
                     /locus_tag="KLF07_001251"
     CDS             33015..35240
                     /gene="ybiO"
                     /locus_tag="KLF07_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBD9831322.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35237..36163)
                     /gene="rlmF"
                     /locus_tag="KLF07_001252"
     CDS             complement(35237..36163)
                     /gene="rlmF"
                     /locus_tag="KLF07_001252"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBD9831323.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36439..36699
                     /locus_tag="KLF07_001253"
     CDS             36439..36699
                     /locus_tag="KLF07_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD9831324.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36964..>37264
                     /locus_tag="KLF07_001254"
     CDS             36964..>37264
                     /locus_tag="KLF07_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD9831325.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9035 a        10059 c         9338 g         8832 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tcttcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 tttaaattta aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca
    28381 ttcatccaga ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag
    28441 tcaaaacggg ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca
    28501 ctccacgctt tacttaagtc tagatatttg tgggagaaag gatgcctggt tcattacgta
    28561 aaatgccggt ctggttacca atagtcatat tgctcgttgc catggcgtct attcagggtg
    28621 gagcctcgtt agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc
    28681 gtctggcatt aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg
    28741 ccaaagagca acggttaccg ctgttgtttt acggcgtttc gctgggtggg atgaattatc
    28801 ttttttatct ttctattcag acagtaccgc tgggtattgc ggtggcgctg gagttcaccg
    28861 gaccactggc ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc
    28921 tggcggttct tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt
    28981 taaccggctg tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg
    29041 ggcaacgcgc aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag
    29101 cgttaatttt cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg
    29161 ttattccatt gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa
    29221 tgattgccct cacccgtttg ccaacacgga catttggtac gctgatgagc atggaaccgg
    29281 cgctggctgc cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc
    29341 tggcgctcgg cgctatcatc gccgcttcaa tggggtctac gctgacagta cgcaaagaga
    29401 gcaaaataaa agaattagac attaattaaa tttacatttc tgcatggtta tgcataacca
    29461 tgcagaattt ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc
    29521 atatttattc cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc
    29581 taactcattg aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata
    29641 ggaacagcca gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg
    29701 gacataacat caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg
    29761 accaatctgc tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag
    29821 ttgctgaatc gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac
    29881 tggaacatgc gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc
    29941 gcactgatcg atcatctgga taccatggca gaacgtgcag tgcagctggg cggtgtagct
    30001 ctggggacca ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc
    30061 cacaacgttc aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac
    30121 gtacgcaaag cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg
    30181 tctcgcgacc tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc
    30241 tgatggtgca gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt
    30301 ttgcagccca cctcaacgca cttatttagt gcatccatct gctatctcca gctgattaag
    30361 taaatttttt gtatccacat catcacacaa tcgttacata aagattgttt tttcatcagg
    30421 ttttacgcta aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc
    30481 agaatggtgc atcttcaggg tattgcccta taaatcgtgc atcacgtttt tgccgcatct
    30541 cgaaaaatca aggagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag
    30601 gggatcgtcc cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact
    30661 ggctgcactg accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt
    30721 cgcgacggat accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt
    30781 tgacgttgat ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc
    30841 gatggatttc agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc
    30901 gggcattacc atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa
    30961 aagcggcctg ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct
    31021 cgacgggaaa gtggttgctg ttaagagcgg tactggctcc gttgattacg cgaaagcaaa
    31081 catcaaaact aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg
    31141 caccaaccgc gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac
    31201 cgccggtaac ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat
    31261 tgcgttcccg aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct
    31321 gcgcgagaac ggaacttaca acgaaatcta caaaaaatgg ttcggtactg aaccgaaata
    31381 ataacgctac acctgtaaag cgcactggca gttccctctc ccctctgggg agaggattag
    31441 ggtgaggggc gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc
    31501 acggtaacag gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct
    31561 tctgattgaa ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct
    31621 ggtaatcgga ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt
    31681 cgcgctggtc tttattgagg tgatccgcgg cacacctatc gtcgtccagg tgatgtttat
    31741 ttatttcgcc ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt
    31801 ggtcaccatc atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct
    31861 gtctattcac aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac
    31921 cattcgctac gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca
    31981 gtggatcatc agcattaaag acacctcgct gtttattgtg atcggcgtgg cggaactgac
    32041 ccgtcagggg caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt
    32101 ggcggtgttc tatctgatta ttaccctggt gctaagcttt attttgcgtc gtctggaaag
    32161 aaggatgaaa atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag
    32221 gtgcttcaca atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg
    32281 tccggttccg gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc
    32341 ggcgatctga ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt
    32401 cgccaggaag caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg
    32461 gaaaacgtca tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa
    32521 ctggcgcgtg agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc
    32581 gaactttctg gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg
    32641 aaaatgatgc tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg
    32701 ctgaaggtta tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa
    32761 atcggttttg ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg
    32821 gaagatggcg atccgcaggt attgatcaag aacccgccga gccagcgctt gcaggaattt
    32881 ttgcagcacg tctcttaact tctctgcctc cctttcaccc gaaagggagg cacaccagat
    32941 tcctctcatt taaaatcgcc cctcccccag catctatact tatctttttg ctctgttttc
    33001 tcactggagg agtcatgcgg tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg
    33061 cccacgcggt atccataccc ggcgttacaa ccacaacgac aacggactca acgactgaac
    33121 cggccccgga accggatatc gaacaaaaaa aagcggccta tggcgcactg gcggatgtgc
    33181 tggataacga cacctcgcgt aaagaattga tcgaccagtt gcgtactgtc gccgcgacgc
    33241 cccctgcgga accggtcccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc
    33301 tgcaaaaggt caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcgggc
    33361 aactttatcg caatatcacc ggctccccgc ataagccatt taatccacaa accttcagca
    33421 atgcgctgag ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc
    33481 gcctgtgcgc actgccgctg tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg
    33541 agcgcagcaa ctggttgcag cttccggcga tgattatcgg ggcgtttatt atcgacctgc
    33601 tgttactggc cctgacatta tttgtcggcc aggtattaag cgacaacctg aatgcaggca
    33661 gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt
    33721 tcaaagccgt actacgcctg attttttgcc caaacgtggc ggagctgcgc ccgttcacga
    33781 ttcaggacga gagcgcccgt tactggagtc gtcgcctgag ctggttaagc agtttgatag
    33841 gttatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta cagataggtg
    33901 cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac ctgatctttc
    33961 gtaataaaaa agagattacc cagcatttgc tcaactttgc ggagcattcg ctggcctttt
    34021 tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta
    34081 tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag cctgaaattt atgatgggtg
    34141 caacggtgcg cagcctggcg attattggta tcgcagcgtt tgtttccggt atgttttccc
    34201 gctggctggc aaaaaccatc actctctcgc cacatactca gcgtaactat ccagagttgc
    34261 aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct
    34321 gcgtggcggt aatgttgctg ttgagcgcat ggggattgtt cgatttctgg aactggctgc
    34381 aaaacggcgc ggggcagaaa accgtagata tcctgatccg tatcgcactt attctttttt
    34441 tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata
    34501 ttcatggccg cccgctaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg
    34561 cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtca
    34621 atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg tttggttcgc
    34681 aaacgctggt gaaagatatc attaccgggg tgtttattca gtttgaaaac ggcatgaaca
    34741 ctggagattt ggtgactatc gggccgttga ccggcacagt ggaacggatg tcgattcgct
    34801 ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca
    34861 cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc
    34921 atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtagcggaa ttaatggaaa
    34981 acgaagaaat tcgcgggctg attattggtg aaccgaattt tgccgggatt gtcggcttaa
    35041 gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg
    35101 tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc
    35161 cagtgcagac ttatcaggtg ctgcctactc cgggcgcgac cccggctgaa ccgttaccgc
    35221 cgggggagcc aacgctttaa cgctggcgat tgacaaaacg gcggcgctgc tcgtcgttca
    35281 taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct ttttttacca
    35341 ccttcaccgc gcccacgtcg gtcagagcac gatacaacgg cggtaagttt tcaccacgag
    35401 ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct
    35461 ttttgataaa ggcgacttca ccaccttcac accacaactc ctgttgctga ccaccaaagt
    35521 tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgctcacta cctgcccgcg
    35581 ctgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt
    35641 tatggatgat gccgttaaaa attgccccac tctctttttg ccgacgcaga cgaatggcgc
    35701 ggttaagccc cggattagca ctgataatcg cctgcgcact gcttaacgcc tggctgctgg
    35761 tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt
    35821 tcgcaccaac gccgatatcc agaatgctgg cattcgctgg aattgttccg cttgcttcag
    35881 ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggt gggcagagaa
    35941 aaccgtcggg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga
    36001 gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctccccggcg ggtgtaagcg
    36061 tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt
    36121 gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc
    36181 agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcattcc
    36241 ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag cattatgcat
    36301 gtcattaatt tgtcttattt tgtaaaaata tatcgttcaa aaaataagca catctctgtc
    36361 aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac tacccgttgt
    36421 atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc
    36481 ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag cggtcagcta
    36541 cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa
    36601 ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga
    36661 aatgaccaga tgttcggtac tgcaaccatc tacaaataac cccaccaacg taactgcatt
    36721 cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata tgaactcaat
    36781 gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgcc aagtgataat
    36841 gcttatcaaa attattatca cttgcatgaa cactatcacg ggattaacag tggcatcgca
    36901 tccgcagaga tgctttctcg tggcagtgaa aatttcaaca tataagaaaa agtcacctgc
    36961 aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc
    37021 ggtctttgta ttggcatcac gcctgtggct caggcactcg ccgccgaagg gcaaactaac
    37081 gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct
    37141 gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct
    37201 gaacaagtga ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa aaacgccccc
    37261 ggcg
//
LOCUS       DAEEBB010000027        36331 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEEBB010000027 DAEEBB010000000
VERSION     DAEEBB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.027"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="KLF07_001255"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="KLF07_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBD9831326.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="KLF07_001256"
     CDS             1867..1992
                     /locus_tag="KLF07_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831327.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="KLF07_001257"
     CDS             complement(1989..2060)
                     /locus_tag="KLF07_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831328.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="KLF07_001258"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="KLF07_001258"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBD9831329.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="KLF07_001259"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="KLF07_001259"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBD9831330.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="KLF07_001260"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="KLF07_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBD9831331.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="KLF07_001261"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="KLF07_001261"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBD9831332.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="KLF07_001262"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="KLF07_001262"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBD9831333.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="KLF07_001263"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="KLF07_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBD9831334.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="KLF07_001264"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="KLF07_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBD9831335.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="KLF07_001265"
     CDS             complement(9465..9872)
                     /locus_tag="KLF07_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HBD9831336.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="KLF07_001266"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="KLF07_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBD9831337.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="KLF07_001267"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="KLF07_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBD9831338.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="KLF07_001268"
     CDS             complement(11538..12197)
                     /locus_tag="KLF07_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBD9831339.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="KLF07_001269"
     CDS             complement(12361..12672)
                     /locus_tag="KLF07_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBD9831340.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="KLF07_001270"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="KLF07_001270"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBD9831341.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="KLF07_001271"
     CDS             complement(14207..14950)
                     /locus_tag="KLF07_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD9831342.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="KLF07_001272"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="KLF07_001272"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBD9831343.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="KLF07_001273"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="KLF07_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBD9831344.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="KLF07_001274"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="KLF07_001274"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBD9831345.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="KLF07_001275"
     CDS             20005..20088
                     /locus_tag="KLF07_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831346.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="KLF07_001276"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="KLF07_001276"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBD9831347.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="KLF07_001277"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="KLF07_001277"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBD9831348.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="KLF07_001278"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="KLF07_001278"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBD9831349.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="KLF07_001279"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="KLF07_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBD9831350.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="KLF07_001280"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="KLF07_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBD9831351.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="KLF07_001281"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KLF07_001281"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="KLF07_001282"
     CDS             25234..25836
                     /locus_tag="KLF07_001282"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBD9831352.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="KLF07_001283"
     CDS             complement(25937..25996)
                     /locus_tag="KLF07_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD9831353.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="KLF07_001284"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="KLF07_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBD9831354.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="KLF07_001285"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="KLF07_001285"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBD9831355.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="KLF07_001286"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="KLF07_001286"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBD9831356.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="KLF07_001287"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="KLF07_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBD9831357.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="KLF07_001288"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="KLF07_001288"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBD9831358.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="KLF07_001289"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="KLF07_001289"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBD9831359.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="KLF07_001290"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="KLF07_001290"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBD9831360.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="KLF07_001291"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="KLF07_001291"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBD9831361.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36331)
                     /locus_tag="KLF07_001292"
     CDS             complement(35716..>36331)
                     /locus_tag="KLF07_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9831362.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9742 c         9170 g         8716 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DAEEBB010000028        35943 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEEBB010000028 DAEEBB010000000
VERSION     DAEEBB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.028"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..694)
                     /gene="tolA"
                     /locus_tag="KLF07_001293"
     CDS             complement(<1..694)
                     /gene="tolA"
                     /locus_tag="KLF07_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBD9831363.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKA"
     gene            complement(759..1187)
                     /gene="tolR"
                     /locus_tag="KLF07_001294"
     CDS             complement(759..1187)
                     /gene="tolR"
                     /locus_tag="KLF07_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBD9831364.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(1191..1883)
                     /gene="tolQ"
                     /locus_tag="KLF07_001295"
     CDS             complement(1191..1883)
                     /gene="tolQ"
                     /locus_tag="KLF07_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBD9831365.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(1880..2284)
                     /gene="ybgC"
                     /locus_tag="KLF07_001296"
     CDS             complement(1880..2284)
                     /gene="ybgC"
                     /locus_tag="KLF07_001296"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD9831366.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(2434..2727)
                     /gene="ybgE"
                     /locus_tag="KLF07_001297"
     CDS             complement(2434..2727)
                     /gene="ybgE"
                     /locus_tag="KLF07_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBD9831367.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(2727..2840)
                     /gene="cydX"
                     /locus_tag="KLF07_001298"
     CDS             complement(2727..2840)
                     /gene="cydX"
                     /locus_tag="KLF07_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBD9831368.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(2855..3994)
                     /gene="cydB"
                     /locus_tag="KLF07_001299"
     CDS             complement(2855..3994)
                     /gene="cydB"
                     /locus_tag="KLF07_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD9831369.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(4010..5578)
                     /gene="cydA"
                     /locus_tag="KLF07_001300"
     CDS             complement(4010..5578)
                     /gene="cydA"
                     /locus_tag="KLF07_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBD9831370.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(6174..6401)
                     /locus_tag="KLF07_001301"
     CDS             complement(6174..6401)
                     /locus_tag="KLF07_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831371.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(6426..9059)
                     /gene="mngB"
                     /locus_tag="KLF07_001302"
     CDS             complement(6426..9059)
                     /gene="mngB"
                     /locus_tag="KLF07_001302"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBD9831372.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(9077..10993)
                     /gene="mngA"
                     /locus_tag="KLF07_001303"
     CDS             complement(9077..10993)
                     /gene="mngA"
                     /locus_tag="KLF07_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBD9831373.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            11162..11884
                     /gene="mngR"
                     /locus_tag="KLF07_001304"
     CDS             11162..11884
                     /gene="mngR"
                     /locus_tag="KLF07_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBD9831374.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(11988..12857)
                     /gene="sucD"
                     /locus_tag="KLF07_001305"
     CDS             complement(11988..12857)
                     /gene="sucD"
                     /locus_tag="KLF07_001305"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBD9831375.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12857..14023)
                     /gene="sucC"
                     /locus_tag="KLF07_001306"
     CDS             complement(12857..14023)
                     /gene="sucC"
                     /locus_tag="KLF07_001306"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBD9831376.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(14117..15334)
                     /gene="odhB"
                     /locus_tag="KLF07_001307"
     CDS             complement(14117..15334)
                     /gene="odhB"
                     /locus_tag="KLF07_001307"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBD9831377.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(15349..18150)
                     /gene="sucA"
                     /locus_tag="KLF07_001308"
     CDS             complement(15349..18150)
                     /gene="sucA"
                     /locus_tag="KLF07_001308"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBD9831378.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(18451..19167)
                     /gene="sdhB"
                     /locus_tag="KLF07_001309"
     CDS             complement(18451..19167)
                     /gene="sdhB"
                     /locus_tag="KLF07_001309"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBD9831379.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(19183..20949)
                     /gene="sdhA"
                     /locus_tag="KLF07_001310"
     CDS             complement(19183..20949)
                     /gene="sdhA"
                     /locus_tag="KLF07_001310"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBD9831380.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(20949..21296)
                     /gene="sdhD"
                     /locus_tag="KLF07_001311"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(20949..21296)
                     /gene="sdhD"
                     /locus_tag="KLF07_001311"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBD9831381.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(21290..21694)
                     /gene="sdhC"
                     /locus_tag="KLF07_001312"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(21290..21694)
                     /gene="sdhC"
                     /locus_tag="KLF07_001312"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD9831382.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            22075..22182
                     /locus_tag="KLF07_001313"
     CDS             22075..22182
                     /locus_tag="KLF07_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831383.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            22388..23671
                     /gene="gltA"
                     /locus_tag="KLF07_001314"
     CDS             22388..23671
                     /gene="gltA"
                     /locus_tag="KLF07_001314"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBD9831384.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            24061..24627
                     /locus_tag="KLF07_001315"
     CDS             24061..24627
                     /locus_tag="KLF07_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD9831385.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            24678..27136
                     /locus_tag="KLF07_001316"
                     /pseudo
     CDS             24678..27136
                     /locus_tag="KLF07_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            27151..27882
                     /locus_tag="KLF07_001317"
     CDS             27151..27882
                     /locus_tag="KLF07_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD9831386.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            27879..28940
                     /locus_tag="KLF07_001318"
     CDS             27879..28940
                     /locus_tag="KLF07_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831387.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            29092..30138
                     /locus_tag="KLF07_001319"
     CDS             29092..30138
                     /locus_tag="KLF07_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBD9831388.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(30135..30926)
                     /gene="nei"
                     /locus_tag="KLF07_001320"
     CDS             complement(30135..30926)
                     /gene="nei"
                     /locus_tag="KLF07_001320"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBD9831389.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(30962..31696)
                     /gene="pxpA"
                     /locus_tag="KLF07_001321"
     CDS             complement(30962..31696)
                     /gene="pxpA"
                     /locus_tag="KLF07_001321"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBD9831390.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(31686..32618)
                     /gene="pxpC"
                     /locus_tag="KLF07_001322"
     CDS             complement(31686..32618)
                     /gene="pxpC"
                     /locus_tag="KLF07_001322"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBD9831391.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(32612..33268)
                     /gene="pxpB"
                     /locus_tag="KLF07_001323"
     CDS             complement(32612..33268)
                     /gene="pxpB"
                     /locus_tag="KLF07_001323"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBD9831392.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(33291..34034)
                     /gene="ybgI"
                     /locus_tag="KLF07_001324"
     CDS             complement(33291..34034)
                     /gene="ybgI"
                     /locus_tag="KLF07_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBD9831393.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            34305..35786
                     /gene="dtpD"
                     /locus_tag="KLF07_001325"
     CDS             34305..35786
                     /gene="dtpD"
                     /locus_tag="KLF07_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBD9831394.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         8746 a         9672 c         8758 g         8767 t
ORIGIN      
        1 ctgccttatc agcagccgct ttttctgctg cttttttgtc ggctgcagct ttctctgctg
       61 ccgctttctt tcttgcttca gctgcagcag cttctgccgc ttccgctttc ttcttcagtg
      121 ccgcagcggc tgcctcggct tttttctgcg cttcggctgc ggctttggcg gcttctgctt
      181 ctgctttttt cttcgcgtct gccgccgctt tcttcgctgc ttcttccgca gctttagcat
      241 ccgcttcggc cttcgcttta gcatctgccg ccgctttcgc tgccgcctct tccgcttgct
      301 tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgt tcctgagcag
      361 ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca
      421 gttcttcagc agcctgctgt tcctttatct tgcgctgctc atcagaacgc ttcgcgcttg
      481 attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag
      541 cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac
      601 tccagagcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct
      661 tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct
      721 gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa
      781 ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa
      841 ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg
      901 gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc
      961 acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc
     1021 accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc
     1081 gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg tacaatgttg
     1141 atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg
     1201 ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag
     1261 ttgtcgtaat tcagttccag tttgtttacg cgctggttga ggcggttata agccattacc
     1321 gccggaattg cggcgaacag accaattgca gtcgcaatca acgcttctgc gataccgggc
     1381 gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc
     1441 ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac
     1501 ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac
     1561 gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct
     1621 ttgaacccgc tgtaaaatat ttgttccgaa ccagtcagat tatcccgttt cccctgactc
     1681 tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct
     1741 tcgcgcgccg ctgcgttaag aatacgggtc cgctggataa taatggccca ggatgcgatt
     1801 gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa
     1861 tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac
     1921 aaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag
     1981 tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta
     2041 tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca
     2101 ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat
     2161 gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca
     2221 caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat
     2281 tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc
     2341 gcgcgattga ggtttgggaa tgagggcaag ttaagggagc gtaaaaattt atgggcctgg
     2401 agcggcccat aattttttaa agaaaattct gatttaaaag aagaagaaaa tcagcccgac
     2461 aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg
     2521 acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg
     2581 ccagatttcc agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc
     2641 tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc
     2701 atacaaggtc gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg
     2761 acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga
     2821 atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt
     2881 caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaagcg gtgtagagca
     2941 gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg
     3001 aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg
     3061 ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa
     3121 acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcgga agaaccacac
     3181 ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag
     3241 cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt
     3301 tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca
     3361 gtgtcaccag cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg
     3421 tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg
     3481 ccagcaggcc gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat
     3541 attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca
     3601 gcggcggaac gaaactacca atgaagatgc cccagtccca catgttacgc cagcgggttt
     3661 cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga
     3721 ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa
     3781 agagtgcgcc gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg
     3841 agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca
     3901 cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc
     3961 accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg
     4021 gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc
     4081 cgaggcgtgc aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc
     4141 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgag ttcgccacag
     4201 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
     4261 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
     4321 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagaa
     4381 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
     4441 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
     4501 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
     4561 ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
     4621 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
     4681 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
     4741 cctcttcctg atcaggaatg ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc
     4801 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
     4861 catcacccag aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
     4921 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga
     4981 ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag
     5041 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
     5101 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
     5161 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
     5221 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
     5281 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
     5341 tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac
     5401 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
     5461 agaccgtttc cataatggcc agcaggaacg ccataccgag cgtcagtggc acaaaaagga
     5521 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
     5581 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
     5641 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
     5701 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
     5761 tacgctgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
     5821 ggtgatcaat ttatagctaa attaccgcct ttcagccaat ttgatcgaga acaatttatc
     5881 tctttttgat gccaatttcc aagacttata cattgataaa tatcaattcc tacaaattat
     5941 gttaatcatg tttcaataag gtaactttag tgacatttat gtttaaaatg tgtgagttat
     6001 aaggctgaaa ttagtttcgg ttggcaattt aaagataaaa agagacgaag caaaaacagc
     6061 acggttgacc ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta
     6121 ctaataaagg cataaatcca ccccgccgat attaacattt aattaacttg taattatatt
     6181 ataattaact cacccagggt tattattttg ttaaaaataa agccacatat acgtggcttg
     6241 agagtgaaga taaacgcaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
     6301 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
     6361 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
     6421 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
     6481 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
     6541 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
     6601 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
     6661 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
     6721 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
     6781 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
     6841 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
     6901 ccaggcctta aaagcagatc ttctttgccc agtaagccca cgccacgcag caacgtaatg
     6961 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtaacccttc gctaaagaca
     7021 gccatgccgt tacgcccttc ctgtaaggca acatagttga gcatattcca taccggaacc
     7081 ggcgcttctt tccagccttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
     7141 gttagcgaac caaactgcgt atctgccaga acactgtcag tgttaaaagg tgtagggacc
     7201 aggacacgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatacgc
     7261 ctgctgttat gactaagagt gacaaccaac acccccccta ctctgccagt ggattgccgg
     7321 gcgctgcgtt ctgacaaatt gagcggcact gccatgtcat agcggataac agccctgctc
     7381 tgccaggctt catgaataat atcgcattgc ggtttcgcgt tcgctgcggt aattacccac
     7441 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
     7501 aatacccggt catagcgcac accgctgtct ttatctacca gttgcagaga accatcctca
     7561 ttgagcgcaa tctgccagaa agcattttcc agaatccctt cagcgtcact ttttgccgca
     7621 atcacgttgc caggctgatt cgcttcaata taaagcgtgc gatagcccat agaagggaca
     7681 atctggttga tctgaatatc aaactccata aagggatcat aattgccata atgaacaatt
     7741 tgccgatcga ttaggccagg atcgatctca cgggcatggc gaataaaata cggtacaggc
     7801 tgaccgcgat cgtcttgtag attaaactgg ctggcgcgca gccgcacagt ggtgttaata
     7861 acttcttccc gcggccaggg catcaggtta aacagcacga gtttgtcggc atcactctgt
     7921 ggcatgttat cggcaatttt gcgcatgtag aaacgcagca gattatccgc catgtcttca
     7981 gccagttcga agcgggcgac aatttcgcga tgaactttgt cactgcaaca acaaccgata
     8041 ctgtcgtggg catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg
     8101 tattcaaaac ccaacgtcca ggccagcgtt gccagcggtt ccagcagatt aacaatctta
     8161 ttttcaatac gtgcgtgggc aattttgata tccatgcgcg tggaaccgat ggtgcgatgt
     8221 acgcgcatat atttgccatc gataaattca cctttcaggg tggccagact ttcacgctgc
     8281 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaacttacg ttgagggtag
     8341 atctcacgta gcttatccat cacttcgaag atattttgct gcaatggcat ctggtcatga
     8401 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
     8461 tcgaggcgtt tacgtaatcc gttttcgtcg gcaggtaagt acttaccgat ggcgtagccc
     8521 agcggcagca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
     8581 ttatcagtac cgtggcgctc cgaacatccg cgccagaaca tagtgcgggt aatgccaaat
     8641 ccattgtaaa tatgcggcag ttgcccggac atgccaaagg aatccggtaa ataacctatt
     8701 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
     8761 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaactta
     8821 ccggcttcta cctgtttctt cacgcggtct ttgttttccg gtttcaccgc gaaataatct
     8881 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
     8941 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
     9001 taccactccc gatcccaatg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
     9061 gtcgtttcct ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg
     9121 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
     9181 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
     9241 agaaaatgcc ggcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
     9301 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
     9361 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
     9421 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
     9481 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
     9541 ccaggcagaa tgcataagcg gctttgttca ctggccctcc aaggtcaaag gaacacataa
     9601 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
     9661 taagcgagtt attgatccat gcgacaggtt ctcccaccac aaacagcatc agactgcccg
     9721 ctcccaacgt accgagcacc gggtagagat aaaaagtcag gaatccattg aatttactgc
     9781 taagacgcaa gtgatttttc acccagcgca tcaagtaacc ggctatcaat ccgccaacga
     9841 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
     9901 ctaacgccgg tttatctgcc agagaatagg tggtataggc cgcgagcacc ggtaccatca
     9961 aaattccgag cagcccgccg cccagcttgc ggtacatcca cagccaggag ttttcttcat
    10021 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtgc
    10081 cccccgcgac aatcaacggt acggcaaaag agattccgct caacagtgcc tgtttcagct
    10141 ccgttttgac acttttcacc ggttgcgtat cttgctgtac ggtacgcgtc tcatcgctac
    10201 gcttgagggt aagcgcttgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    10261 gcactgaaag cgcgggaatg ccattaaaac gctcactctc cttgatggcg acttcagccg
    10321 caaaaataca ggcggttgca ctattgagtt gatccgccgt taaacgccct tcaatgccgt
    10381 tagcgccttg tttttcaacg taaacattta cgccgagttt gcgtccggct ttttccagat
    10441 attccgcagc catataggtg tgagcaatac ccgccggaca ggccgttacg cagacgatag
    10501 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    10561 gcaactcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgcaaggc
    10621 gcgtggtcag cgctgtcagc agttgcatat gcgtagtacc cgcttcattg gggggaatcg
    10681 ccagcagcac cactaaatca actgcttccg ggccatcaac gccttcccac tgaagcggct
    10741 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    10801 cagccaaccc ttcacctaag gcggtcgggc caaggctttc acggcgatac acttcttcca
    10861 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgga
    10921 tggcctcttc acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag
    10981 tcgtcagatt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgat
    11041 aaaacaatac catcatgtga tcaatttcac ttgattgaaa ttatttgtat tgtatttgta
    11101 ttatttacag gatgaattta tcgggcataa tggctaaatt catattaatg agatactaaa
    11161 aatgggacac aagcccttat accggcagat tgccgatcgc attcgtgaac aaattgcgcg
    11221 tggcgagctt aaacctggcg atgcgttacc cactgaatca gcgctacaaa cagagtttgg
    11281 cgtcagccgg gttacggtgc gtcaggcttt gcgccagcta gtcgagcagc agatcctcga
    11341 aagcattcag ggcagcggga cttacgtcaa agaagagcgg gtcaattacg atatttttca
    11401 gttaaccagt tttgatgaaa aactgtcgga ccgtcacgtc gatacacaca gtgaaatcct
    11461 gatatttgaa gtgattccgg ctgacgattt tcttcagcaa cagctacaaa tcaccgcgca
    11521 ggatcgtgtg tggcatgtga agcgcgtgcg ctatcgcaag caaaagccaa tggcgctgga
    11581 agaaacctgg atgccgcttg cgttgttccc ggatctcacc tggcaggtca tggaaaattc
    11641 gaaatatcac tttattgaag aagtgaagaa gatggttatc gatcgtagcg aacaggaaat
    11701 cattcctctg atgccaaccg aagagatgag ccgtctgctt aatatcagcc agacaaagcc
    11761 gattcttgaa aaagtatcgc gtggatattt agttgatggt cgggtgtttg aatatagccg
    11821 caacgctttt aataccgatg actataaatt taccctgata gcgcagcgaa aatcatcgcg
    11881 ataagcacaa aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    11941 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    12001 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    12061 agagcagcga atttctcatc cgcagtccct ttcccaccag caatgatcgc acccgcgtgg
    12121 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacctacaac tggcttggta
    12181 acgtgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    12241 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgaggatgtc gataaagtta
    12301 gagcctggga tcgggtcacc gccgatacca acacaggtcg actgaccgaa accgtaatcc
    12361 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    12421 cccggtttgt gaatgtgacc cggctggata ccgattttgc attcacccgg agtgataacg
    12481 cccgggcagt tcgggccgat catacgaacg ccggcttcgt ccagtttcac tttcacagtc
    12541 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatca
    12601 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtagataac agaagcggta
    12661 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    12721 gtaccgcctt tacctggggt tacgccgcca accattttag tgccgtatgc aatggcctgt
    12781 tctgagtgga aagtcccctg gctaccagta aagccctggc agataacctt ggtgttttta
    12841 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    12901 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    12961 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tctgctaccg
    13021 cgccgatgat accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca
    13081 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    13141 tggttgcgcc gccgccaacg tcaaggaagt tagccggttc gccgccgtgc agtttaacga
    13201 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    13261 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    13321 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagtgc gttgccgtca gcgcccagtt
    13381 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    13441 acgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    13501 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    13561 ggccagtcag cggatcaagc gcaactttgt ggatcaggtg cggagtttct tccgccactt
    13621 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacacgacgg gaactacggt
    13681 ctacaacggc accgagatac agctctttag cgatatcggt cgctgcttca accagaatct
    13741 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg ccgagccagt
    13801 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    13861 cgcggccacc agcgtgaacc tgacatttca ctacccacgg gccggcaccg atttttgaag
    13921 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    13981 agcgggcaaa aagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    14041 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    14101 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggatcttcc agcaactctt
    14161 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    14221 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    14281 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    14341 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    14401 tcagcttgcc gtcacggcct ttaactgcca gctctttgat tttcttctcg atgtctgcca
    14461 tgccgagggt atcgacatca cgcagtaccg gcgtcaccag gccgcgcggc gtggaaaccg
    14521 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc accgtcgata gaagcattca
    14581 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    14641 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    14701 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    14761 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    14821 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    14881 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    14941 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    15001 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    15061 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    15121 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    15181 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    15241 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    15301 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    15361 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    15421 cggagaggcg gaggccgtgc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    15481 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    15541 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    15601 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    15661 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    15721 atcaagctcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    15781 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacta ccagcggacg
    15841 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    15901 aacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    15961 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    16021 ccggccccat ttctgttcgc cagaagagat gaactggtcg ataaccacct gcgcaccgtt
    16081 ggcgaagtca ccgaactgcg cttcccagat ggtcagagtg cgtggttctg cggtggcata
    16141 accgtattca aacgccagca ctgcttcttc agacagtacg gagtcccaga cgcggaacgc
    16201 gccctgcccg ttatggatgt gttgcaacgg cgtgtaagtg gaaccgttag actggttgtg
    16261 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    16321 cggaatgcct tcgtcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    16381 cagtttctca ccggcagcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    16441 catttcaact gcttccggca ccgtgctgat gcgtttcgcc agctcctgca ggcgcttcat
    16501 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    16561 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    16621 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    16681 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagcgg
    16741 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc acaccaggtc
    16801 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    16861 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    16921 atcagtacag tacggtgtag aacgagcatc cagtggatta gaggtggtga aaccaacctg
    16981 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    17041 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    17101 cagcactttg ttgctgctcg gctcatcaag tctgtccaga cgggcacgaa cagaaccgat
    17161 aactaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    17221 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cacccgtgcc
    17281 gaggtgttct ttatgtttac cggcaaactc gtcgaacaag tcttgcggtt ttttacccag
    17341 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccaagaacca cttcgcgggt
    17401 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    17461 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgtt caaggccttc
    17521 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    17581 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtaatgt gcatatactc
    17641 ggcaccaatc gggccacagt aggtttgctt gagggcttcc agcagctcgc cgagtttcat
    17701 gttttctttg ccgctggcaa atgaaccgac gttgaaggtc tcctggaagt ctgcttcggt
    17761 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    17821 atcgagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    17881 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    17941 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    18001 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    18061 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    18121 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    18181 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    18241 ttgtctatgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    18301 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctacgg
    18361 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    18421 gacgcttgcg cgtcttatca ggcctacggt ttacgcatta cgttgcaaca acatcgactt
    18481 aatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    18541 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    18601 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    18661 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    18721 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    18781 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    18841 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac
    18901 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    18961 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    19021 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    19081 ttcttccgct tccagggggt aatcctgcat acgcggagca tcatcaacat ccgggttata
    19141 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    19201 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    19261 cgactctggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    19321 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    19381 cgccgtttcc atcaggttat ccagttccaa gcactcaacg cgctgggtgt tgaactcgct
    19441 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    19501 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    19561 cagcgcttta cgaatcgcca ccggatcttc accgttacga ttattgttcc agcggtgcag
    19621 acgatccaga gatgcttcaa catcagactc gctggcatcg cgcaatgcgc cctgttcggc
    19681 gatagactct tgcagatgca gacctgccgc acgaccaaag accaccaggt ccagcagcga
    19741 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    19801 cagccccgga acaaccacat cttcgccttt ctcgttcacc gtcagcgcct gaccggtcac
    19861 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    19921 caccggatcg acgtgagcaa aggtacggga aagctccagg atacccggca gacgggattc
    19981 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    20041 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    20101 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    20161 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    20221 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    20281 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgcccc
    20341 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    20401 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    20461 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    20521 gtgaccggta cggtcagccg ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    20581 cgactgaccg ccaaacggac gttgatagat acgaccatca tcaagacgcg agaacggcag
    20641 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    20701 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    20761 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    20821 acgggtcggg aagactttag agagcagcgc acaggtctgg ccgctctggg aaatttgcag
    20881 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    20941 caatttcatc acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    21001 acgacaatca ccagttgcag catcaggcgc aatgccagcg gtttaacgta gtcggtcaac
    21061 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    21121 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    21181 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    21241 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    21301 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    21361 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    21421 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    21481 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    21541 gccacagcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    21601 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    21661 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    21721 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    21781 gcttgcgggt cagacagcgt cctttctata actgcgcgtc atgcaaaaca ctgcttccag
    21841 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    21901 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    21961 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    22021 aatttgacaa tcattcaaca aagttgttac aaacattgcc aggaaaagca tataatgcgt
    22081 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    22141 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    22201 acttgattcg ccatttattc gtcatcaatg gatcctttac ctgcaagcgc ccagagctct
    22261 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    22321 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    22381 accttaaatg gctgatacaa aagcaaaact caccctcaac ggggacacag ctgttgaact
    22441 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    22501 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    22561 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    22621 gaccgattct aactacctgg aagtttgtta catcctgctg aatggtgaaa aaccgactca
    22681 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    22741 tacccgtctg ttccatgctt tccgtcgcga ctcgcatcca atggcagtca tgtgtggtat
    22801 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcaccg
    22861 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    22921 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    22981 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    23041 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    23101 cgtgcgtacc gctggctctt cgggtgcgaa cccgtttgcc tgtatcgcag caggtattgc
    23161 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    23221 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    23281 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    23341 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    23401 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    23461 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    23521 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    23581 aatgcacagt gacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    23641 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    23701 acccgccgtt caaatggcgg gttaattttt atatgcccaa acacaaaaaa ttgtaaaaat
    23761 aaaatccact atcggacatt catatattct aaaaaaaata tatcggtata aattataaac
    23821 aaatcgatga tttttaatca tctatcttcc tctcagttac atgaataaac caccaaatat
    23881 agcgcacccc tataatttcc aatactttat tcatcaaaca tttaattcat accatcaatt
    23941 aattactttt acatttaata aaattaataa tatttttaag aaaaactaaa aacattcgac
    24001 aatcaaataa gatattcttg caccgatata cttttttctt ttctgttaaa ggactcagtt
    24061 atgtttaaag gacaaaaaac attggccgca ctggccgtat ctctgctgtt cacagcacct
    24121 gtttatgcgg ctgatgaagg atccggtgaa attcacttta aaggtgaagt tattgaagca
    24181 ccgtgtgaaa tacatcagga tgatattgat aaagaggttg aactcggtca ggtgaccacc
    24241 agccacatta atcagtcaca tcacagcgat gccgttgctg tcgacctgcg cttagtcaac
    24301 tgtgatctgg aaaactccag caacggttcc ggtggcaaga tttctaaagt tgcagtcacc
    24361 tttgatagct cagcgaaaac caccggcgca gatccgattc tcaacaacac cagcacgggt
    24421 gaagccaccg gcgtcggcgt acgtttaatg aataaagatc aaagcaatat cgttttaggc
    24481 accgctactc cagatatcga cctggctccg acctccagcg aacaaacgct gaatttcttt
    24541 gcctggatgg aacaaattga tcaggctaca cctgtaacgc caggcgcagt tacggcgaat
    24601 gcaacctacg tgctggatta taaataagta ttactttggc ggcgctatcg ccgcctttca
    24661 taaaatattt atcaggtatg gacaccgtga atatttatcg actctctttt gtatcctgcc
    24721 tggtcgtggc gatgccttgc gcattggcgg tcgaattcaa ccttaatgtt ctcgataaat
    24781 cgatgcgcga ccgcattgat atttcattat taaaggaaaa aggagtcatt gctcccggtg
    24841 agtattttgt tagcgttgcg gtaaataata accaaatcag taacgggcaa aagattaact
    24901 ggcacaaaaa tgacgataaa accattccgt gcatcaatga tttactggtc gataaatttg
    24961 gcttaaaacc tgaagtccgt cagtcgttac cattgataaa tcagtgcgtc gattttagct
    25021 cccgacctga aatgctcttc aatttcgatc aagccaatca gcaactgaat attagtattc
    25081 cgcaagcctg gctggcgtgg cactcagaaa actggacccc accctccaca tggaaagaag
    25141 gtgtcgccgg tatcctgatg gattacaact tgtttgccag cagctaccgc ccacaggacg
    25201 gcagcagcag cactaacctg aacgcctacg gtaccaccgg aattaacgcc ggggcatggc
    25261 gcttacgtag tgattatcag ttgaatcaga ctgatagcga tgataaccat gaacagtcag
    25321 gcggaatatc gcgcacctat ctttttcgtc cattaccgca attaggctct aaattaaccc
    25381 tcggcgaaac ggattttagt tccaatattt tcgacggttt ttcttatacc ggcgcggcac
    25441 tggcaagtga cgagcgaatg ttgccatggg aactacgcgg ctacgcccca caaattagcg
    25501 gtattgcaca gaccaatgcc acggtgacga tcagtcaatc aggccgcgtc atttaccaga
    25561 aaaaagtccc accaggccca tttatcattg acgaccttaa tcagtctgtt cagggcacac
    25621 tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa tttccaggtt tcggcagcat
    25681 cgacgccctt cctgactcgt cagggacagg ttcgctataa actggccgcg ggtcagccac
    25741 ggccctccat gtcacatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg
    25801 ggatgctgtc aaacacctcg ctgtacggcg gcctgctgct ttctggtgat gactaccatt
    25861 ctgccgcaat gggtattggg caaaatatgc tgtggcttgg tgcgctgtcg tttgatgtca
    25921 cgtgggccag tagccatttt gatactcagc aggacgagcg gggcttaagc taccgtttta
    25981 attacagcaa acaagtggat gccactaaca gcacgatttc cctcgccgct tatcgtttct
    26041 ccgatcgtca ttttcacagc tacgccaact atctggatca caaatacaac gacagcgatg
    26101 cgcaggacga aaaacagacg atcagcttat tgtgggccaa ccgattaccc cactaaacct
    26161 caatctttac gccaacctgc tacatcaaac ctggtggaat gcagacgcct ccacgaccgc
    26221 caacatcacg gcaggtttta atgttgatat tggtgactgg agagatatct ctatttcgac
    26281 gtcattcaat acaacccatt acgaagataa agatcacgac aaccagattt acctgtcgat
    26341 ttcgctcccc ttcggtaacg gtggtcgggt tggctatgac atgcaaaaca gtagccacag
    26401 caccacacac cgcatgtcgt ggaacgatac gctggatgaa cgtaatagct ggggcatgtc
    26461 tgccggactg caatccgacc gtcctgacaa tggagcccag gtgagcggta actatcagca
    26521 cctgagttca gcgggtgagt gggatatttc tggtacctat gccgccaatg attacagttc
    26581 cgtcagcagc agctggagcg gttctttcac cgcaacccaa tatggtgcag cgtttcatcg
    26641 ccgcagctcc accaatgaac cacgcctgat ggtcagcacc gatggcgtgg cagatattcc
    26701 ggttcagggc aatctcgact acaccaacca ttttggcatt gcggtggtgc cgttgatttc
    26761 cagttaccag ccttccaccg tggcggtgaa catgaatgac ttacccgacg gcgtaacagt
    26821 tgcagaaaac gttatcaaag aaacgtggat tgaaggcgcg ataggttaca aatcactggc
    26881 ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac gccagcggtc agtttcctcc
    26941 cctcggagcg gatatccgcc aggatgacag cggcattagc gtggggatgg ttggcgagga
    27001 aggacatgcc tggttaagcg gagtcgctga aaatcaaaag tttaccgtgg tctggggtga
    27061 tagccagcat tgctcgctcc atcttcctga acatatggaa gacaccgcaa atcgcctgat
    27121 tttaccttgt cattaattaa ggaaatgaaa atgacattta tgaaaggact accccttttg
    27181 ttgttggttg ccagtctgtg cagccacgct gcactacaac ccgatcgcac tcgtattgtg
    27241 tttaacgcta atgataaagc taccagtctg cgggtggata atcgtagcga taaactcccc
    27301 tacctcgctt attcctggct cgaaaatgaa aaaggggaga aaagcgatga tcttctggtc
    27361 gccctgccgc cgatacaacg tctggaaccg aaagcaacaa cgcaggtacg gatagtaaag
    27421 caagcctcga ccacaaaatt accgggcgat cgcgaaacgc tatttttcta caatatgcgt
    27481 gaaattcctc ccgccccgga aaaaaacagc gaccatgccg ttctgcaagt cgccattcag
    27541 agtcgtatca aggtgttctg gcgaccggct gcattacgca agaaagcagg agaaaaggtc
    27601 gaactgcagt tacaggtcag ccagcagggc aaccaactga cactgaaaaa tcctaccgcg
    27661 tattatctga ccattgccta cctgggacga aatgaaaaag gcgttctccc cggcttcaaa
    27721 accgtgatgg ttgcgccttt tagtacggtc aacacaaaca ccggaaatta tagcggcagc
    27781 cagttttatc tcggttatat ggatgattac ggcgcgttac gcatgacgac gctcaactgc
    27841 agcggacaat gccatttaca agccgtggag gcgaagaaat gagtgctggc aagggagtgt
    27901 tgctcgtcat ctgtctgtta tttctgccgt tgaagtctgc gcttgccctg aactgctact
    27961 ttggttcatc cggtggctcg gtagaaaaat cagaagcgat tcaacctttt gctgtaccag
    28021 gcaatgccaa actcggcgat aagatttggg aatctgacga tattaaaatt cccgtctatt
    28081 gtgacaacaa taccaacggc aattttgaaa gtgagcatgt ttatgcctgg gtaaacccct
    28141 acccgggggt gcaggatcgc tattatcaac tcggcgtaac ttacaatggc gttgattatg
    28201 acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg tattgacagt aagaacattg
    28261 acatttatac ccctgagcag attatcgcga tgggatggca gaacaaaatt tgctcgggtg
    28321 atcccgctaa tattcacatg tcgcgtacct ttctcgctcg catgcggtta tacgtcaaaa
    28381 tacgagaaat gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat
    28441 ttgacggtgc cggtagcgtt aacgaagacc ctactgccca aaacctgaaa tatcatatta
    28501 ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa
    28561 cacaggtgat tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga
    28621 aaacattcag tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag
    28681 tcagttcctc cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg
    28741 gtaatggtct gaagctacgt ttgctggatg aaaacgcctc gccttatacc ttcaataaat
    28801 atgctgaata cgccgatttc accagcgaca tgttggtcta cgaaaaaacc tatacagctg
    28861 aactttcgtc cattgcaggt acccccatcg aggctgggcc cttcgataca gtggtgcttt
    28921 ttaaaattaa ctataactga tttgccatga tgccctttgc agccggaaag ggcatctctt
    28981 tcagaaaatt gctgattttc cccttttcct tgattgctgt tattttttcc tggcccggct
    29041 ttctttatgc tgtatcgata caccaaagag aaaataatga gggagcgaag gatgccagtt
    29101 ttgcagtggg gaatgttatg tgtgctatca cttctccttt ctattggttt cctcgcggta
    29161 catctcccgg cggcgctatt acttgggccg atgatcgccg ggatcatctt cagtatgcgc
    29221 ggaattactc tgcaacttcc ccgctccgct tttctcgccg cgcaggccat tcttggctgc
    29281 atgattgcgc aaaacctcac tggttcgatt ctcaccacgc tggccgccaa ctggccgatc
    29341 gtgcttgcta ttttactggt gacgctgctt tccagcgcca tcgtgggttg gttattggtg
    29401 cgctatagct cactgcctgg aaataccggt gcctggggtt cttctccagg cggcgcagcg
    29461 gcaatggtcg ctatggcgca agattacggc gcagatattc gcctggttgc atttatgcaa
    29521 tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg tcacccgcat gatgctgggt
    29581 gataacgctg aagcggttaa ccagcaaatc gtctggttcc cgccagtgag cattaatctc
    29641 ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct
    29701 tccggcacga tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc
    29761 accatcgaac taccggaatg gctgctggcg atggcgtata tggcaattgg ctggcggatt
    29821 ggtcttggtt tcgataaaca aatcttactg cgggcactac gcccgctacc gcaaatcctg
    29881 ctgtcgattt ttgctctgct ggctatttgt gcgggtatgg cgtgggggct gacccggttt
    29941 atgcacattg attttatgac tgcctacctc gccaccagtc ccggcgggct tgataccgtg
    30001 gcggtcatcg ccgcagggag caatgccgat atggcgctta ttatggcgat gcaaaccctg
    30061 cgcctgttca gtattttgct gacggggcca gccattgcgc ggtttatttc aacctatgcg
    30121 ccgaagcggt cggcctagtg ctggcagcca gggcaccagt aaaacgggcg agatgacagc
    30181 gtggttttct caatgatgct gccacagcgt tcgcacggtt cgccatcacg atgaaaaacc
    30241 ttaaagcgaa acagcgcccc atgatgctta ttctcatcca ccagccccct cgtagcgtag
    30301 gaaaatcgag gaatatccag taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga
    30361 tctttcgctt tatgatttcc agtcaaccca acctgccaga ggatctccac ccgcaaataa
    30421 ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc ctgcaaactg acggttacga
    30481 aagcgcggtg acaataaccg ttcctttacc acctccggcg tcagattcgg atccagcaca
    30541 tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt gttccggcct caacatctca
    30601 atatccgagg cgctataaag cagaatggtt ttgtcagccg tttgcagttt tacccgcaat
    30661 actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc gccagacgcc gtaaagctga
    30721 ttatggctgt agagcgttaa gtcgttggaa aaatgagtta acaacgcctt accacgcgtt
    30781 tccacatggg taacgtgttg accgataagt tgtgattgat aagtttttaa ctgcgggaag
    30841 gcaaaccaga catcagttag tggtttgcct ttgatcgccg cctccaggtt atccgctgca
    30901 cggcggatct ccgggccttc aggcatggtg atatcctttt ttcattcttt attaatcggg
    30961 gttatgctgc gacaacaatc tccttttcgg caaatgtaga gcgtagtcgg cgggcgaacg
    31021 ccagtgcgtg ctcgccgtcg ccatgcaggc agactgtttg cgccgtgacc gttgcccatt
    31081 cgccggtgat acttttgact ctgccgtgtt gcaccatctc tagcgtttgc gccaacgcct
    31141 gttcttcgtt ttcaatcaac gcgcctcgct gacttcgcgg caccagcgag ccgtcagcct
    31201 gataaccgcg atcggcaaac acttcctcgc gcgttgtcag gccatattgc ttgcctgcac
    31261 gaatcagctc gcttcctgcc agcccaacga gaatcaatgc cggatcgcaa gcgtataccg
    31321 ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac aacatgccgt
    31381 gcggtttgac atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa
    31441 tttgatacag cgtctgggcg taaacggttt ctggcggcag ctgcatggcg ctgcgaccaa
    31501 aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc gacaccattt tttattgctt
    31561 cacgcacgca agcctgcatg atttgcgcat cgcctgcatg aaatccacag gcaatattgg
    31621 cagaggaaac cagcgttaat agctctgcgt cgctggcgca gccttcgccc agatcggcgt
    31681 tcaggtcaat tttcattgtg cagccgccac actaattgtt cgaaataacg ttgctgatct
    31741 tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc
    31801 ggaatttgcg ccagatggta catatcagcc tcaatgatac aggcaatacg cgggtaacca
    31861 tcggtggtct gtgcgtcgtt catcaacaca atcggctgcc cgttatgtgg cacctgcacc
    31921 acgcccggta acaaaccgtg agataacagt tcgcgatcgg tggtgcgttt taaaatttgc
    31981 ccctgtaagc gatagcccat gcggttactt tgcgagctaa gctgccaggg cgaacgccag
    32041 aatgcatcct gcgaggcgcg atcgaactca tgatattccg gccccggcaa ggcgcgaatg
    32101 cggttgcccc acagcagctg tttaacgccc tgcgcttcca taaaatcacg cttcgatttg
    32161 ccaatcggga gtcggtcacc atccttcagt aaacggcctt ccagcccgcc aatccccact
    32221 ttgagatcgg tgctgcatga ccccattacc ggcggaacat caataccacc cgcgaccgcc
    32281 agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc
    32341 ggcaatcgcc agccggtcca gacggcatta tcatccagcc gcgcttcgca accggcaccc
    32401 gtcagagcaa accacccatc agtttcgaac tcaaccgtta actgaccgag cgtgatctcc
    32461 agcgcggggg cattggcgtc attacccacc agcaggttag caatgcgtaa cgcaggcata
    32521 tccagtgcgc cgcagtggct gattcccgac tggcgaaaac cgtgacgacc gccatcctgc
    32581 acagtggtat acatgcccgc acgaataatc ttcagcatac tccctccttc tgcggtacaa
    32641 agcgcacgct gtctcccgga cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc
    32701 tgagtgaggt atgaccaatc aactgccagc cacccggcgt tgccagcgga taaacaccag
    32761 tctgcggccc gccgatcccg acagaacctg ccggaacgag caagcgcggt tcagcgcgcc
    32821 gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta
    32881 aaaaccagac cacgtattcc acggaggagt gcaattcaac aacctgtttt tcgctaaacc
    32941 cgcaatgcgc cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa accaccggaa
    33001 tttcaataaa gcgagactca ggttccaggg cctcgctctc ctcccaccag cgttgcaaac
    33061 gctctatggc atccagcgcc agcgactcag gattacgcaa aatcaccgtg atattgttca
    33121 tgccgggaat ggcttcaacc acattcggca tatccaccag acgctgcgcc agtcgccaga
    33181 tccgtttctg gctagccagc gtcaccggcg gttccagttc cagcactacc gccgtttcac
    33241 ctatcagata acaacgcgct cgttgcactt tcgatccctc tgattattcg ttatgcagga
    33301 ttaggaatat caataaaggt cacatcaaga tcggtatttt cattcagcca ctcgctcaat
    33361 gcgcgaatac caccacgttc agtggcatgg tgacctgcag cataaaaatg caacccctgc
    33421 tcgcgggctg aatgaatggt ctgttcagaa acttcgccag taataaaagc atccacgcca
    33481 aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc aacgcgctgg
    33541 accacctcag gtccggtatc gccacaccat aacggcttac gtcccagacg cgcttcaatc
    33601 caggaggcta gttccagtcc cggcacaggc atggtcagct ctccccacgg caccaacggc
    33661 tcaatttcgc ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt attgcccagc
    33721 tcaggatgta cgtcaagcgg caaatgccag ccatacaggt tgatatcatt cgccagcaac
    33781 gtttttaaac ggttacgctt cattccgcga atgaccggag actctccttt ccagaagtag
    33841 ccatgatgca caatgaccgc atccgcgccc agacgcactg cctcatcgag caaagcctgg
    33901 ctggcggtga caccggtaac aattttttgc accgtctctt tgccttctac ctgcaaacca
    33961 ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt cgttaatcag ttgttccagt
    34021 tcggtgtttt tcatcttcgc gccttccgta ttctgatagc tacaccgtta acatagcgtt
    34081 acctcgcctg ttcgtcgata gccaatcttt tgacatatta agaaacaaac ccgacgccaa
    34141 aagacgcaaa ttgtttatat catcacgctt cagctaatgt atatttatgc aatcagacag
    34201 catattgcat aaataatcag atctgtatca aagttttaac tttaaggaaa cacaagcacg
    34261 ccgcgcaact attacgtgac gttgcagtca attgtggaat tattatgaat aaacacgcat
    34321 cacaaccgcg cactatttac tatgtcgttg cgctgcaaat ctgggaatat tttagctttt
    34381 acggcatgcg tgccctgctg attctctatc tcaccaatca actaaaatac aacgatactc
    34441 acgcctacga gttatttagc gcctactgtt cgctggtgta tgtcacgcca atcctcggtg
    34501 gctttttggc ggataaagtt ctcggcaatc gcatggcggt gatgctgggg gcgttgttga
    34561 tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca tccgtcattc ctctatctgt
    34621 ccctggcgat tatcgtttgt ggctatggtc tgtttaaatc caatgtcagt tgcctgctcg
    34681 gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg tttctcgctg atgtatgcag
    34741 cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca
    34801 gctgggcgat gggctttggc ctggcggcgg tgggtatgat cgcgggtctg gtcattttct
    34861 tatgtggcaa tcgtcatttc acacataccc gcggcgttaa taaaaaagta ctgcgtgcaa
    34921 caaactttct cctgcctaac tggggatggc tgctggttct gctggtggca acgcctgcgc
    34981 tgattaccgt actgttctgg aaagagtggt cggtatacgc cttaattgtc gcgactatta
    35041 ttggcctggg tgtactggca aaaatttatc gcaaagcaga aaaccagaaa cagcgtaagg
    35101 agctggggct gattgtgacg ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac
    35161 agggcggcag ctcaattagc ctttatatcg accgcttcgt taaccgcgat atgtttggtt
    35221 ataccgttcc gactgctatg ttccagtcga ttaatgcctt cgcagttatg ctatgcggtg
    35281 tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg
    35341 gaaaatttgc tcttggcctt ggcctgatga gcgccggatt ctgcattctg accttaagcg
    35401 cccgctggtc cgcaatgtat ggtcactctt ctctgccact gatggtgtta ggcctcgcgg
    35461 tgatgggctt tgcggaactg tttatcgacc cggttgccat gtcgcaaatt acgcgcattg
    35521 aaattccggg cgtgaccggc gtattaaccg gcatctacat gctgctttct ggcgcgatcg
    35581 cgaactatct ggcgggcgtg attgccgatc agacatcgca ggcttcgttt gatgcttccg
    35641 gggcgatcaa ctactccatc aatgcatata ttgaagtatt tgatcaaatt acctggggcg
    35701 cactggcgtg tgtaggactg gtactgatga tttggctgta tcaggcgctg aaattcagaa
    35761 accgcgcgct ggcgctggag tcataatagg ccagcaccga tgcctgatgc gacgcttgac
    35821 gcgtcttatc aggccggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
    35881 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggg
    35941 ggt
//
LOCUS       DAEEBB010000029        35778 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEEBB010000029 DAEEBB010000000
VERSION     DAEEBB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.029"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..581)
                     /locus_tag="KLF07_001326"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="KLF07_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="KLF07_001327"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="KLF07_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBD9831395.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="KLF07_001328"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="KLF07_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBD9831396.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="KLF07_001329"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="KLF07_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBD9831397.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="KLF07_001330"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="KLF07_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBD9831398.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="KLF07_001331"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="KLF07_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBD9831399.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="KLF07_001332"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="KLF07_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBD9831400.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="KLF07_001333"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="KLF07_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBD9831401.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="KLF07_001334"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="KLF07_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBD9831402.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="KLF07_001335"
     CDS             complement(10994..11137)
                     /locus_tag="KLF07_001335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831403.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="KLF07_001336"
     CDS             11138..11416
                     /locus_tag="KLF07_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831404.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="KLF07_001337"
     CDS             complement(11463..13559)
                     /locus_tag="KLF07_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD9831405.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="KLF07_001338"
     CDS             complement(13559..14296)
                     /locus_tag="KLF07_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD9831406.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="KLF07_001339"
     CDS             complement(14293..14931)
                     /locus_tag="KLF07_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD9831407.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="KLF07_001340"
     CDS             complement(15045..15287)
                     /locus_tag="KLF07_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831408.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="KLF07_001341"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="KLF07_001341"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBD9831409.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="KLF07_001342"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="KLF07_001342"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBD9831410.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="KLF07_001343"
     CDS             19524..19796
                     /locus_tag="KLF07_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBD9831411.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="KLF07_001344"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="KLF07_001344"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBD9831412.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="KLF07_001345"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="KLF07_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="KLF07_001346"
     CDS             22056..22862
                     /locus_tag="KLF07_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD9831413.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="KLF07_001347"
     CDS             22872..23279
                     /locus_tag="KLF07_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBD9831414.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="KLF07_001348"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="KLF07_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="KLF07_001349"
     CDS             23839..24636
                     /locus_tag="KLF07_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBD9831415.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="KLF07_001350"
     CDS             24647..25471
                     /locus_tag="KLF07_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBD9831416.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="KLF07_001351"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="KLF07_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27824
                     /gene="pepE"
                     /locus_tag="KLF07_001352"
                     /pseudo
     CDS             27134..27824
                     /gene="pepE"
                     /locus_tag="KLF07_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27915..29546)
                     /locus_tag="KLF07_001353"
     CDS             complement(27915..29546)
                     /locus_tag="KLF07_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBD9831417.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29766..33449)
                     /gene="metH"
                     /locus_tag="KLF07_001354"
     CDS             complement(29766..33449)
                     /gene="metH"
                     /locus_tag="KLF07_001354"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBD9831418.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33649..34473
                     /gene="iclR"
                     /locus_tag="KLF07_001355"
     CDS             33649..34473
                     /gene="iclR"
                     /locus_tag="KLF07_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBD9831419.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34790..35778
                     /gene="espL4"
                     /locus_tag="KLF07_001356"
                     /pseudo
     CDS             34790..35778
                     /gene="espL4"
                     /locus_tag="KLF07_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9231 c         9102 g         8847 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagctaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaatcaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg ggggcgggaa tactttccag ttgctgaaac
    27421 agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt
    27481 atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata
    27541 tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg caaatcaacc
    27601 cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc
    27661 gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga
    27721 tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg
    27781 ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat tgccggatgc
    27841 atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta
    27901 caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca
    27961 ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa
    28021 tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg
    28081 tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg
    28141 ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc
    28201 tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc
    28261 gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg ttcaaggttg
    28321 agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag ttcctctttt
    28381 ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc
    28441 ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc
    28501 atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc
    28561 gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc
    28621 ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg
    28681 accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc
    28741 aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta
    28801 cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc attggcggca
    28861 ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc
    28921 gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca
    28981 aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg
    29041 ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc
    29101 tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt
    29161 tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac
    29221 ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct
    29281 gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag
    29341 gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg
    29401 caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg
    29461 aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca
    29521 gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg
    29581 aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt
    29641 gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt
    29701 aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt
    29761 gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg
    29821 ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg
    29881 tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc aggccacatg
    29941 gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg
    30001 gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg
    30061 tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag
    30121 actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc
    30181 gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg
    30241 tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc
    30301 ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga
    30361 cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg
    30421 tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt cagcgttttc
    30481 tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg
    30541 cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag
    30601 aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg
    30661 ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg
    30721 cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt
    30781 acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc
    30841 agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg
    30901 tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt
    30961 aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc
    31021 gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg
    31081 agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc
    31141 agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc
    31201 accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag
    31261 gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttcccc
    31321 tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca
    31381 atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg
    31441 cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc
    31501 tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa
    31561 cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca
    31621 gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca
    31681 ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg ttcgttgcca
    31741 cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt
    31801 ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg
    31861 gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct
    31921 tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc
    31981 tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc
    32041 gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg
    32101 ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc
    32161 ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct
    32221 tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc gttttccacc
    32281 tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag gcgcttgaac
    32341 ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcttcgcca
    32401 atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg cagtttgcgc
    32461 ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag
    32521 cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg ttttgccatc
    32581 gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg
    32641 gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatcgggc
    32701 cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat
    32761 gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccagagatc
    32821 ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa taccgccgct
    32881 ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc
    32941 agcgctttgg tggactctcg ataagccacc accagcccgt caaaagtgat attacgaaat
    33001 gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg
    33061 gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc
    33121 agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc
    33181 gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag
    33241 gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct
    33301 ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga
    33361 tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc
    33421 gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta
    33481 ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc
    33541 aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattaacta
    33601 acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc
    33661 attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt
    33721 cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg
    33781 gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta
    33841 accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc
    33901 ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcgatt
    33961 gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg
    34021 cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac tcacctgatg
    34081 cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc
    34141 tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat
    34201 gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg
    34261 cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg ttgccttgca
    34321 gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt
    34381 tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag
    34441 gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt
    34501 ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa
    34561 aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc
    34621 aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc
    34681 attatgagat tgagcgattt atgatatttc tttagttgta atcatattaa attttttcac
    34741 tgttctgata gttaaaattt aagacatcaa taaacaatga gatatttaaa tgattactcg
    34801 tattcctcgt agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca
    34861 aaccctgagt gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc
    34921 gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa aatggtcgtc atatctttag
    34981 caacaaggat tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat
    35041 tacgataatt aataaaacga aaacacgaca ctcactcaaa ccattgctcc aatatttgaa
    35101 gaatacctaa tggaaatttt acctcaacgc tcagacactc ttgataaaca agaattaaac
    35161 ctaaaatcag atagaaaaga aaaagaattc ccaagaatta aacttaatgg tcaatgctat
    35221 tttccggggc gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca atatattaat
    35281 gatatttatc agaatgttga ttacaaaccc catcaagatg attattcttc agctgaaaaa
    35341 tttctcactc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt
    35401 cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt
    35461 gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat
    35521 gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt
    35581 tcggtttatg atcctaatgt aactaacgac aaaataagaa tcatgagcga aagcaaagag
    35641 gatattaaac actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg
    35701 tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag
    35761 ctattgatat taaaagga
//
LOCUS       DAEEBB010000030        34971 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEEBB010000030 DAEEBB010000000
VERSION     DAEEBB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.030"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..805)
                     /locus_tag="KLF07_001357"
     CDS             complement(<1..805)
                     /locus_tag="KLF07_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaH family type III secretion system E3
                     ubiquitin--protein ligase"
                     /protein_id="HBD9831420.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="KLF07_001358"
                     /pseudo
     CDS             897..983
                     /locus_tag="KLF07_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1387..1863)
                     /locus_tag="KLF07_001359"
     CDS             complement(1387..1863)
                     /locus_tag="KLF07_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBD9831421.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1922..3211)
                     /gene="bioA"
                     /locus_tag="KLF07_001360"
     CDS             complement(1922..3211)
                     /gene="bioA"
                     /locus_tag="KLF07_001360"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBD9831422.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3298..4338
                     /gene="bioB"
                     /locus_tag="KLF07_001361"
     CDS             3298..4338
                     /gene="bioB"
                     /locus_tag="KLF07_001361"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBD9831423.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4335..5489
                     /gene="bioF"
                     /locus_tag="KLF07_001362"
     CDS             4335..5489
                     /gene="bioF"
                     /locus_tag="KLF07_001362"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBD9831424.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5476..6231
                     /gene="bioC"
                     /locus_tag="KLF07_001363"
     CDS             5476..6231
                     /gene="bioC"
                     /locus_tag="KLF07_001363"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBD9831425.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6224..6901
                     /gene="bioD"
                     /locus_tag="KLF07_001364"
     CDS             6224..6901
                     /gene="bioD"
                     /locus_tag="KLF07_001364"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD9831426.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7480..9501
                     /gene="uvrB"
                     /locus_tag="KLF07_001365"
     CDS             7480..9501
                     /gene="uvrB"
                     /locus_tag="KLF07_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBD9831427.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9693..10601)
                     /gene="yvcK"
                     /locus_tag="KLF07_001366"
     CDS             complement(9693..10601)
                     /gene="yvcK"
                     /locus_tag="KLF07_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBD9831428.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10866..11010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10998..11987
                     /gene="moaA"
                     /locus_tag="KLF07_001367"
     CDS             10998..11987
                     /gene="moaA"
                     /locus_tag="KLF07_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBD9831429.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12009..12521
                     /gene="moaB"
                     /locus_tag="KLF07_001368"
     CDS             12009..12521
                     /gene="moaB"
                     /locus_tag="KLF07_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBD9831430.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12524..13009
                     /gene="moaC"
                     /locus_tag="KLF07_001369"
     CDS             12524..13009
                     /gene="moaC"
                     /locus_tag="KLF07_001369"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBD9831431.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13002..13247
                     /gene="moaD"
                     /locus_tag="KLF07_001370"
     CDS             13002..13247
                     /gene="moaD"
                     /locus_tag="KLF07_001370"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBD9831432.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13249..13701
                     /gene="moaE"
                     /locus_tag="KLF07_001371"
     CDS             13249..13701
                     /gene="moaE"
                     /locus_tag="KLF07_001371"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBD9831433.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13838..14542
                     /locus_tag="KLF07_001372"
     CDS             13838..14542
                     /locus_tag="KLF07_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD9831434.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14747..15460
                     /locus_tag="KLF07_001373"
                     /pseudo
     CDS             14747..15460
                     /locus_tag="KLF07_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15525..16236)
                     /locus_tag="KLF07_001374"
                     /pseudo
     CDS             complement(15525..16236)
                     /locus_tag="KLF07_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16236..17477)
                     /gene="clsB"
                     /locus_tag="KLF07_001375"
     CDS             complement(16236..17477)
                     /gene="clsB"
                     /locus_tag="KLF07_001375"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBD9831435.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17474..18235)
                     /locus_tag="KLF07_001376"
     CDS             complement(17474..18235)
                     /locus_tag="KLF07_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD9831436.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18368..18778
                     /locus_tag="KLF07_001377"
     CDS             18368..18778
                     /locus_tag="KLF07_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBD9831437.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18740..19846)
                     /locus_tag="KLF07_001378"
     CDS             complement(18740..19846)
                     /locus_tag="KLF07_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9831438.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19857..20990)
                     /locus_tag="KLF07_001379"
     CDS             complement(19857..20990)
                     /locus_tag="KLF07_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9831439.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20983..22719)
                     /locus_tag="KLF07_001380"
     CDS             complement(20983..22719)
                     /locus_tag="KLF07_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9831440.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22712..23707)
                     /gene="hlyD"
                     /locus_tag="KLF07_001381"
     CDS             complement(22712..23707)
                     /gene="hlyD"
                     /locus_tag="KLF07_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBD9831441.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23710..24381)
                     /gene="cecR"
                     /locus_tag="KLF07_001382"
     CDS             complement(23710..24381)
                     /gene="cecR"
                     /locus_tag="KLF07_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBD9831442.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24610..25974
                     /gene="rhlE"
                     /locus_tag="KLF07_001383"
     CDS             24610..25974
                     /gene="rhlE"
                     /locus_tag="KLF07_001383"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBD9831443.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26206..26688)
                     /gene="ybiA"
                     /locus_tag="KLF07_001384"
     CDS             complement(26206..26688)
                     /gene="ybiA"
                     /locus_tag="KLF07_001384"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBD9831444.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26808..28958
                     /gene="dinG"
                     /locus_tag="KLF07_001385"
     CDS             26808..28958
                     /gene="dinG"
                     /locus_tag="KLF07_001385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBD9831445.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28986..29948
                     /gene="ybiB"
                     /locus_tag="KLF07_001386"
     CDS             28986..29948
                     /gene="ybiB"
                     /locus_tag="KLF07_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBD9831446.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30089..31174
                     /gene="hcxB"
                     /locus_tag="KLF07_001387"
     CDS             30089..31174
                     /gene="hcxB"
                     /locus_tag="KLF07_001387"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBD9831447.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31403..31663)
                     /locus_tag="KLF07_001388"
     CDS             complement(31403..31663)
                     /locus_tag="KLF07_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD9831448.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31928..32194)
                     /gene="ybiI"
                     /locus_tag="KLF07_001389"
     CDS             complement(31928..32194)
                     /gene="ybiI"
                     /locus_tag="KLF07_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBD9831449.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32268..32945)
                     /gene="ybiX"
                     /locus_tag="KLF07_001390"
     CDS             complement(32268..32945)
                     /gene="ybiX"
                     /locus_tag="KLF07_001390"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBD9831450.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32987..34971)
                     /locus_tag="KLF07_001391"
                     /pseudo
     CDS             complement(32987..34971)
                     /locus_tag="KLF07_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8269 a         8976 c         9484 g         8242 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata tattaatatg
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccactgca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg gaaggtaaaa
     1321 agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg ccatctggca
     1381 gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga aatgaacgtt
     1441 aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta tatccagcgc
     1501 gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc catcgtaccc
     1561 ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga
     1621 gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta cccagtgcca
     1681 ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac ttttcgttcc
     1741 cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc
     1801 gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac tgatgagttt
     1861 catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg cggacttctc
     1921 gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg gtcagacgct
     1981 gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg ccaaaaggcc
     2041 ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc accggatgag
     2101 tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt tcggcatcac
     2161 gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt tgctgccagt
     2221 cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc ggattgccca
     2281 taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt tctgcaacct
     2341 cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct ttaccgaggc
     2401 acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg gtacgaccaa
     2461 atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat attttgcgga
     2521 ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg acaatcggct
     2581 caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca aagcccacca
     2641 tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca aacaagtttt
     2701 ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac accgacatcg
     2761 cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg cgcgcttcgc
     2821 ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct acggaaccgg
     2881 agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt ttgcggcaca
     2941 gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg gcatcaattt
     3001 gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc gcccaccagg
     3061 acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa ccttcggcgc
     3121 tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc cagatatggc
     3181 gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat
     3241 ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag aagccccatg
     3301 gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa accgttgctg
     3361 gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg tcaggtccag
     3421 gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa atactgcccg
     3481 caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt tgaacaggtg
     3541 ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat gggcgcggcg
     3601 tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca gggggtaaaa
     3661 gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca ggcgcagcgc
     3721 ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc ggagttttac
     3781 ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga aaaagtgcgc
     3841 gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac ggtaaaagat
     3901 cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag cgtaccaatc
     3961 aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt cgatgccttt
     4021 gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta cgtgcgcctt
     4081 tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat ggctggcgca
     4141 aactcgattt tctacggttg caaactgctg accacgccga atccggaaga agataaagac
     4201 ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat
     4261 aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga cgaatattac
     4321 aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat gcacggcgta
     4381 ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc tggctggtgg
     4441 cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta agccatcatc
     4501 cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt agcggcggtt
     4561 ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa ctggccgagt
     4621 ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat caggcagtca
     4681 ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc catgcctcct
     4741 tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat aacgatgtcg
     4801 ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg gtgacagaag
     4861 gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag gtaacgcaac
     4921 agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc ggggagcagg
     4981 ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg acttttggca
     5041 aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg gattatctgc
     5101 tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag gcgcaggcat
     5161 tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc gaaaaactgg
     5221 tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg cttgctgatt
     5281 catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta caactggcag
     5341 aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc gtacccgctg
     5401 gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat atcgaccgtc
     5461 tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc atttggtcgg
     5521 gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga cgtcttactg
     5581 gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg gccaggctgg
     5641 atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct ctcaccgcca
     5701 atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg agatatcgaa
     5761 tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc ggtgcagtgg
     5821 tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc cggaggcgtg
     5881 gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc gtggcaggcg
     5941 gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga acagtcgctg
     6001 aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga tgatgcgctc
     6061 agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg gcgcgacccg
     6121 cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca acaacagggg
     6181 cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta aacgttattt
     6241 tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac ttttacaagc
     6301 cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg gcagcgaaaa
     6361 gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca gcagcctgca
     6421 gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc cgcacatcat
     6481 cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat tacgcgcgct
     6541 tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta cgccgctttc
     6601 tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga tactggtagt
     6661 tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa tacaacacgc
     6721 cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa aacgtcacgc
     6781 tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag agatcccctg
     6841 gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg ccttgttgta
     6901 gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac tgcaatttaa
     6961 ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg cggcgaatgt
     7021 gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta ttcctgtgga
     7081 taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc ggcttgcacg
     7141 cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg gttagataaa
     7201 tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac tcatcttgac
     7261 aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt ttatctcgcc
     7321 acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga aatattatgg
     7381 tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg acgagtaaaa
     7441 ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc gttcaaactg
     7501 aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct cgaagagggg
     7561 ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg gaaaaccttc
     7621 accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc gcccaacaaa
     7681 acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa cgcggtggag
     7741 tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag ttccgacact
     7801 ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt atccgccacc
     7861 aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc gatttatggt
     7921 ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg tatgattatc
     7981 gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa tgatcaagca
     8041 ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc ggcagaatcg
     8101 gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt gtcgttattt
     8161 gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta cccgaaaacg
     8221 cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa agaagaactg
     8281 gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca gcggctgacc
     8341 cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc ggggattgaa
     8401 aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac gctgtttgat
     8461 tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat tccacaaatt
     8521 ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta cggcttccgc
     8581 ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc attagcgccg
     8641 caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc cggcggcgat
     8701 gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga agtgcggccg
     8761 gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc aattaacgaa
     8821 cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga atatctcgaa
     8881 gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga acgtatggag
     8941 attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa cttactgcgc
     9001 gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga caaagaaggc
     9061 ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg taacgttaac
     9121 ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc gattggcgaa
     9181 accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat tacgccgcaa
     9241 ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc caaaaccaaa
     9301 gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc gatggatatg
     9361 tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat gcaacacgcg
     9421 cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca gctgcgtgag
     9481 ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa gcaaaattgc
     9541 ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat tagcgcggtt
     9601 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac tttgtcttca
     9661 ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa cgccttttcc
     9721 agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc ctccagtacc
     9781 tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc gacgatgacc
     9841 gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct ttccagcttc
     9901 aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac catcggcgct
     9961 ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat caggctggta
    10021 taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg aaccgcctca
    10081 cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt taactggtcg
    10141 atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag atcgacggga
    10201 tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat taaattgatg
    10261 gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt tccgagatta
    10321 tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga ggcgacgctc
    10381 ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat gccgccttct
    10441 gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat acccgttaaa
    10501 cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc tccgccgaga
    10561 gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc tggagtcaga
    10621 ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc caatattgag
    10681 gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc cctgatgatg
    10741 tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac gatcatgacg
    10801 ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta cacgctagta
    10861 tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac ggtgctttgg
    10921 ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact tcgcctcccg
    10981 tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt aagttttact
    11041 acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc ctgccggatg
    11101 gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa attcgccggg
    11161 ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga ggagagccgt
    11221 ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac gctatccgcc
    11281 agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac tggcgcgatg
    11341 cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag tttcacgcta
    11401 ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca tttgaggtcg
    11461 gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat caccagctcg
    11521 acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc gaactgatgg
    11581 aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag gttctgcgtg
    11641 acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac ggtcctgcgc
    11701 aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg tatgaaaaag
    11761 acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc catctctgcc
    11821 tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc cagcaacagg
    11881 cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat ttcctgcatc
    11941 aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac gtcaaaagga
    12001 gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct attcttacgg
    12061 tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc gattcggcgc
    12121 aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc tacgctattc
    12181 gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg attacggggg
    12241 ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg ttcgaccgtg
    12301 aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt ggcacttcca
    12361 cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc gccatgccgg
    12421 gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag ctggatgccc
    12481 gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc aactgaccca
    12541 tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg aaaccgtgcg
    12601 tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga tgattattga
    12661 tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta ttcaggcggc
    12721 aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca aagttgaagt
    12781 caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat gccgcctgac
    12841 cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg cgctgaccat
    12901 ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac gtttgctggc
    12961 gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa gttctttttt
    13021 tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg gattacccaa
    13081 ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg ctggcgctgg
    13141 aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac catccgctga
    13201 ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat ggcagaaacc
    13261 aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg gctggctgag
    13321 cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca taacctgggt
    13381 gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa agcactggca
    13441 gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt gattcaccgc
    13501 atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag tgcgcatcgc
    13561 agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg cgcaccgttc
    13621 tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga gagcgatcag
    13681 caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg cgtccggcaa
    13741 tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc attttgcagt
    13801 atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc cacgttctga
    13861 ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct atggctggat
    13921 gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt ccgcggccgt
    13981 gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg cgcaattagc
    14041 attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa cgacgatgct
    14101 ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca ttgtctatac
    14161 cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg caatgagcct
    14221 gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt ttatggcgtt
    14281 aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag cattgatgtg
    14341 ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg acactcagaa
    14401 attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc gcaaatattc
    14461 cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga tgttgttacg
    14521 gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca tgtgtcggga
    14581 tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg ccttatgagc
    14641 gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt ggttttaaat
    14701 ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg aatcatactc
    14761 gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg gtatatggct
    14821 cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta agtatctcga
    14881 aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt tattattact
    14941 ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg cgggcgtaat
    15001 atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc ctgtatatgc
    15061 cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc tggccggttt
    15121 attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt tgacagggtt
    15181 ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt gggtaataag
    15241 ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata agctccttga
    15301 actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg cttttacagg
    15361 tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg cgatagctat
    15421 aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc acatactaaa
    15481 gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag tacaaagaaa
    15541 tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat ccagttaacg
    15601 ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag cttttgccct
    15661 ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac cgcgataatc
    15721 atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca gtgatccggc
    15781 aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc cagccagttg
    15841 gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc cagacgagag
    15901 tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag gttgaaggcg
    15961 tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc gcagtaaaag
    16021 cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag cgaccgcact
    16081 aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc agtccacttt
    16141 tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg tgaggatctt
    16201 ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc cgtgttttca
    16261 gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac ctgcgccaga
    16321 cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa aaagtggaac
    16381 gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt ttcatccacc
    16441 tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg gttaaaatta
    16501 cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg atcgagatta
    16561 ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg gagcgggcgg
    16621 cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag caagcgcgcg
    16681 ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat ccgcaccccg
    16741 cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa gtaggcgttg
    16801 gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg ttctatatca
    16861 tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc cggctggcgg
    16921 ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg tccaggcagg
    16981 ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg taccgcgtaa
    17041 tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt cagcccgcca
    17101 ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg aaacacattg
    17161 gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac gccagctgcc
    17221 gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc cagcaagact
    17281 tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg tttgccgacg
    17341 ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc gccaatcgcc
    17401 ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt attgccttcg
    17461 cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc
    17521 gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg acgtagatcc
    17581 tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggcgc
    17641 gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct ttttgccgcc
    17701 agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca ttcacccatt
    17761 cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga tgtacgcaca
    17821 tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct
    17881 tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga taacgcgaca
    17941 gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag gcaaaatcgc
    18001 tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt tccacatgca
    18061 gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg gcgctgacgg
    18121 tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt
    18181 tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg ggcataattt
    18241 tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa aaggtgcggt
    18301 cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat tcttcaggag
    18361 agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt gctggcagat
    18421 tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta agactctggt
    18481 tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt
    18541 tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag agctgaccac
    18601 gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa
    18661 caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg tagtgtcttt
    18721 gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac gccgctgacc
    18781 ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata tccagactcg
    18841 catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg
    18901 tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg gaaaggagaa
    18961 tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc gagcagagtg
    19021 atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc gtaaagtaga
    19081 acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc gccagcacaa
    19141 tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg aagatctgcc
    19201 aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt tcgcgggcga
    19261 cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc agcgacggca
    19321 ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc agctcgctgt
    19381 tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg atctgttgca
    19441 ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc aactgcaacg
    19501 gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc accagtagta
    19561 acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc agcagcacat
    19621 gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc tcgccgttat
    19681 cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg aacgggaaca
    19741 ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt tcgcgcagca
    19801 acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc ttctccctaa
    19861 tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc cgaagcgatc
    19921 aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa caggctttgc
    19981 agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat caccgcgggc
    20041 atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa cgcggcgttc
    20101 agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag cagccccatc
    20161 cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag cgaaccgcga
    20221 tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc cagcatcccg
    20281 agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat ctccgtagag
    20341 agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc
    20401 gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg ctggctaatc
    20461 gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa agtctgcccg
    20521 ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata cccctgtaca
    20581 aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg
    20641 gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg aattttcccc
    20701 gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc
    20761 gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc cagcaagatc
    20821 ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa cagcagtagc
    20881 agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg ccgcgtctct
    20941 ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat tgctatgctc
    21001 cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat cgggttgctc
    21061 atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta atttcccacg
    21121 gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg tggtgaccat
    21181 caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac ggcgggtgag
    21241 gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat gcatcagcga
    21301 acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg cgtgggaggc
    21361 gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct gcgcccgacc
    21421 gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca ggttaccgta
    21481 gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag aactctcttt
    21541 cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac cgcacatcat
    21601 cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa tctccccgtg
    21661 tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct tggtcagttc
    21721 tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata ttgcgcccag
    21781 cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt caaaacgcgg
    21841 cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat cgtctggtgt
    21901 ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt cgctgacctg
    21961 cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac ttgtcatcag
    22021 aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat acagcaactc
    22081 gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat acgaggtact
    22141 ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca gttcgcgccg
    22201 tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg gttcgcccac
    22261 cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc ccgccaggcg
    22321 tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt gcttacgtgc
    22381 ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca ccgtgagatc
    22441 ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt
    22501 tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca gtaatcccgc
    22561 cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc ccgtcacata
    22621 accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc ccggaaagcg
    22681 tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg ttaccgaatt
    22741 gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc acaatacgca
    22801 ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg gtaaattcag
    22861 cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga ccatcggtat
    22921 aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca acataagcgc
    22981 gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca ttgaggaccg
    23041 tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc aacgttgagt
    23101 cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg ctggctttcg
    23161 cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta tcctgtgctg
    23221 atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg tcatttgccg
    23281 aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc gcatagtcat
    23341 aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca tcgcgatacc
    23401 cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc gcctgcatca
    23461 gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct ttgatagcat
    23521 caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta agattcaccg
    23581 tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt tgataccacc
    23641 agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc acgacaggtt
    23701 ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga tatgacaggt
    23761 caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa
    23821 cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg catgggtatg
    23881 aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca ggcgtgtcag
    23941 gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga
    24001 gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg tgagcagctt
    24061 aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat caggctgtgg
    24121 ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc caataaaatc
    24181 ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt agtaggtgat
    24241 ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt tcattccata
    24301 ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt cacccttgat
    24361 tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg attaagatta
    24421 tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca gtcacaggga
    24481 taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac attgtggttt
    24541 ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc ggggcggttc
    24601 ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg cgccgttgcc
    24661 gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc ggtgctggaa
    24721 ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg ctttacgctg
    24781 ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg tccggtacgt
    24841 gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa cgtccgtgat
    24901 tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag tattaacccg
    24961 cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg acgtttgctg
    25021 gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt cctcgatgaa
    25081 gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt aacaaaacta
    25141 cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat taaagccctg
    25201 gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa taccgcgtct
    25261 gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga attgctgtcg
    25321 cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac caaacacggc
    25381 gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc gatccacggc
    25441 aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg cgatattcgt
    25501 gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct gccgcacgtc
    25561 gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg gcgtaccggt
    25621 cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca caaactgctg
    25681 cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc gggctatgag
    25741 ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg tggcggcggc
    25801 ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga aggtggcgca
    25861 aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga tgccaaaccg
    25921 gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca gtaatctttt
    25981 atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct gatgcgctac
    26041 gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt gtaggccgga
    26101 taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa tttgagaaca
    26161 cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact tctctatagc
    26221 caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct taccattacc
    26281 accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg gcgcggttgc
    26341 cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg ctttacgcat
    26401 cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat tacgccccat
    26461 gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt tttcatcaag
    26521 gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt caaccttgat
    26581 gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt agaaatttat
    26641 gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta aaccgcatta
    26701 tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc ctgaaaaccg
    26761 aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg gcattaaccg
    26821 ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag atccccgact
    26881 ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg ctggccggag
    26941 aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg ctctcctatt
    27001 tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca
    27061 acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa aagatcattc
    27121 ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg cgtaatctga
    27181 cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt gacgacgaac
    27241 tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca
    27301 cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat gatctctggc
    27361 gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac tatcgtgaat
    27421 gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg gcaaaccatg
    27481 cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa aatctgctgc
    27541 tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg gagatgagcg
    27601 ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa ctggtcgcta
    27661 cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct gaacgtttga
    27721 atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt ctcaatctct
    27781 acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg cctgatgaag
    27841 tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt
    27901 tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc ctgcatcggt
    27961 tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc
    28021 tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc
    28081 gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc gatcagctgg
    28141 aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc
    28201 tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg ggcgaccgtt
    28261 ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt attccccgga
    28321 tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg gcggcctttt
    28381 tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt gccagcggac
    28441 gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg ctggttcagg
    28501 gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc aacggtgagc
    28561 gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa ggtgatctgc
    28621 tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc gtggtgatca
    28681 ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa agcctgccga
    28741 gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac ggttgctggg
    28801 gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg
    28861 atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata gttaaaaaga
    28921 aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt
    28981 cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg
    29041 atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt gaagtccctg
    29101 accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg gaagcagaga
    29161 tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg ccagcaggca
    29221 agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc aacctgacgc
    29281 cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac ggggttagcg
    29341 aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt acgccaacgt
    29401 tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt atgccagtcg
    29461 gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta
    29521 acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg gcgctgcgtc
    29581 tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt agcgatatcg
    29641 gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat ccgcagcgct
    29701 gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa aaacaggaca
    29761 ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg cagtggattg
    29821 agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag atggtttgct
    29881 gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc gttaatcagg
    29941 cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc ccaccgcaat
    30001 atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata aaaatacaac
    30061 aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg atgctcagac
    30121 gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa
    30181 attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac atggtattgg
    30241 catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta accatcatgc
    30301 caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg catttggtca
    30361 ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc acggtattgc
    30421 cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg cggagcagtg
    30481 tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc caatggtcgc
    30541 gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg ttttccctcg
    30601 taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat ttggcaaaac
    30661 ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg acgttaacgg
    30721 cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt
    30781 tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg gggcgctttc
    30841 cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca ttcttaactg
    30901 catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta acgcgcagac
    30961 cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc cgattttgct
    31021 accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga ttccactgga
    31081 tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc cggaagagac
    31141 gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag tggcggacgg
    31201 gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt cggcaaacag
    31261 acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac ggttcggatt
    31321 ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta
    31381 aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt aatttattgt
    31441 tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct ttctcagcca
    31501 gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg ccgattttat
    31561 tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca aatgacagag
    31621 ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat ctctcgcagg
    31681 tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag
    31741 agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata tctaacatac
    31801 tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg cagtcacagc
    31861 agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg catctcgcca
    31921 gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct gtataagctg
    31981 gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca ggaatggctt
    32041 cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg ctttcgccgc
    32101 gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg atctgttcgt
    32161 tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat caatacttgt
    32221 acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca gatctccgac
    32281 cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc actttcgccg
    32341 tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc gcgcttttta
    32401 tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc gcgggttacg
    32461 ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc cgggagtttt
    32521 acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta gctttgtgga
    32581 tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg tggatggctg
    32641 cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg attaaacagc
    32701 ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg
    32761 ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac ctgttgattg
    32821 ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca ttcggcttgt
    32881 tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg aatgtggtac
    32941 atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag aaatgcatat
    33001 tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg ctcttgttga
    33061 ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag tcgagattgc
    33121 gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta aatgctggcg
    33181 ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg cccgcgccaa
    33241 cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc tccggggtat
    33301 acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg gttgcttttt
    33361 gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc acggatatct
    33421 catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca ttttgctcaa
    33481 cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt ttatccagaa
    33541 cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt
    33601 tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct
    33661 gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac agcgccccgg
    33721 ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt
    33781 ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag gcggtggcac
    33841 tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca aaatcacggg
    33901 tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg
    33961 cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa atatttaccg
    34021 cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta aattccacgc
    34081 cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg ttggtctggt
    34141 tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag gttcagctat
    34201 tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc gtcatcaggt
    34261 aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta tcgttgatgt
    34321 cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc
    34381 cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt cccgcagatg
    34441 gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg ttgtgctggg
    34501 tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa gcgacagaag
    34561 gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc
    34621 ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg tccagcgtgc
    34681 cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg
    34741 gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag tcggtgccgg
    34801 acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag acgctgccga
    34861 tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc atataaatgg
    34921 cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg c
//
LOCUS       DAEEBB010000031        33999 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEEBB010000031 DAEEBB010000000
VERSION     DAEEBB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.031"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            12..514
                     /locus_tag="KLF07_001392"
                     /pseudo
     CDS             12..514
                     /locus_tag="KLF07_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="KLF07_001393"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="KLF07_001393"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBD9831451.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="KLF07_001394"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="KLF07_001394"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBD9831452.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="KLF07_001395"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="KLF07_001395"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBD9831453.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="KLF07_001396"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="KLF07_001396"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBD9831454.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="KLF07_001397"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="KLF07_001397"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBD9831455.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="KLF07_001398"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="KLF07_001398"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBD9831456.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="KLF07_001399"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="KLF07_001399"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBD9831457.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="KLF07_001400"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="KLF07_001400"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBD9831458.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="KLF07_001401"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="KLF07_001401"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBD9831459.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="KLF07_001402"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="KLF07_001402"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBD9831460.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="KLF07_001403"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="KLF07_001403"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBD9831461.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="KLF07_001404"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="KLF07_001404"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBD9831462.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="KLF07_001405"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="KLF07_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBD9831463.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="KLF07_001406"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="KLF07_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBD9831464.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="KLF07_001407"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="KLF07_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBD9831465.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="KLF07_001408"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="KLF07_001408"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBD9831466.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="KLF07_001409"
     CDS             complement(20046..21878)
                     /locus_tag="KLF07_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBD9831467.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="KLF07_001410"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="KLF07_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="KLF07_001411"
     CDS             complement(22624..23118)
                     /locus_tag="KLF07_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBD9831468.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="KLF07_001412"
     CDS             complement(23201..23656)
                     /locus_tag="KLF07_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBD9831469.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="KLF07_001413"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="KLF07_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBD9831470.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="KLF07_001414"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="KLF07_001414"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBD9831471.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="KLF07_001415"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="KLF07_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBD9831472.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="KLF07_001416"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="KLF07_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBD9831473.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="KLF07_001417"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="KLF07_001417"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBD9831474.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="KLF07_001418"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="KLF07_001418"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBD9831475.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="KLF07_001419"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="KLF07_001419"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBD9831476.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="KLF07_001420"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="KLF07_001420"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBD9831477.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="KLF07_001421"
     CDS             32232..33125
                     /locus_tag="KLF07_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBD9831478.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="KLF07_001422"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="KLF07_001422"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBD9831479.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8480 a         9279 c         8563 g         7677 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAEEBB010000032        33995 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEEBB010000032 DAEEBB010000000
VERSION     DAEEBB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.032"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="KLF07_001423"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="KLF07_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="KLF07_001424"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="KLF07_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBD9831480.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="KLF07_001425"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="KLF07_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBD9831481.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="KLF07_001426"
     CDS             complement(4834..5013)
                     /locus_tag="KLF07_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBD9831482.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="KLF07_001427"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="KLF07_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD9831483.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="KLF07_001428"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="KLF07_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBD9831484.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="KLF07_001429"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="KLF07_001429"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBD9831485.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="KLF07_001430"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="KLF07_001430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBD9831486.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="KLF07_001431"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="KLF07_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBD9831487.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="KLF07_001432"
     CDS             complement(10017..10259)
                     /locus_tag="KLF07_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBD9831488.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="KLF07_001433"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="KLF07_001433"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBD9831489.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="KLF07_001434"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="KLF07_001434"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBD9831490.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="KLF07_001435"
     CDS             complement(13176..14150)
                     /locus_tag="KLF07_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD9831491.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="KLF07_001436"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="KLF07_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBD9831492.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="KLF07_001437"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="KLF07_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBD9831493.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="KLF07_001438"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="KLF07_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBD9831494.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="KLF07_001439"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="KLF07_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBD9831495.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="KLF07_001440"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="KLF07_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBD9831496.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="KLF07_001441"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="KLF07_001441"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBD9831497.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="KLF07_001442"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="KLF07_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBD9831498.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="KLF07_001443"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="KLF07_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBD9831499.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="KLF07_001444"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="KLF07_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="KLF07_001445"
     CDS             27953..28156
                     /locus_tag="KLF07_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBD9831500.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="KLF07_001446"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="KLF07_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBD9831501.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="KLF07_001447"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="KLF07_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBD9831502.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="KLF07_001448"
     CDS             31161..31433
                     /locus_tag="KLF07_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBD9831503.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="KLF07_001449"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="KLF07_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBD9831504.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="KLF07_001450"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="KLF07_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBD9831505.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="KLF07_001451"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="KLF07_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD9831506.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8256 a         8454 c         8899 g         8386 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccatat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAEEBB010000033        33398 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEEBB010000033 DAEEBB010000000
VERSION     DAEEBB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.033"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..2119)
                     /locus_tag="KLF07_001452"
     CDS             complement(<1..2119)
                     /locus_tag="KLF07_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD9831507.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPLMEGRSPVIWDKEHKLWF
                     ARPGADLSLLDRWRPRPHEISTDAADPVSEFAQKLEEAGLVLNELPVMDGKIHRVSTT
                     EDRKGEKSGAYKAFLDGRPSGWYRDYRSNDNSPTKWVFSGGKQTDPQAKLHLKAYSLQ
                     RKEDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRINNQNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPILFAEGYATAASL
                     HEATGLPVLMTVDAGNMISIAENVRQLWKQSPFIFCADNDHARQVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     DDETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATTASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPF
                     LPESDGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIE
                     PAVRDEPTPQQPVQSTSPSDNTSS"
     gene            complement(2209..2760)
                     /locus_tag="KLF07_001453"
     CDS             complement(2209..2760)
                     /locus_tag="KLF07_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBD9831508.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAASRKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            complement(2775..3065)
                     /locus_tag="KLF07_001454"
     CDS             complement(2775..3065)
                     /locus_tag="KLF07_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBD9831509.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(3062..4210)
                     /gene="traJ"
                     /locus_tag="KLF07_001455"
     CDS             complement(3062..4210)
                     /gene="traJ"
                     /locus_tag="KLF07_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBD9831510.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDALFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCAALYCYIKKNYPNAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     ILGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(4207..5025)
                     /gene="traI"
                     /locus_tag="KLF07_001456"
     CDS             complement(4207..5025)
                     /gene="traI"
                     /locus_tag="KLF07_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBD9831511.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWSIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(5022..5480)
                     /gene="traH"
                     /locus_tag="KLF07_001457"
     CDS             complement(5022..5480)
                     /gene="traH"
                     /locus_tag="KLF07_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBD9831512.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHILTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQTENRKK"
     gene            complement(5875..6459)
                     /locus_tag="KLF07_001458"
     CDS             complement(5875..6459)
                     /locus_tag="KLF07_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD9831513.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGVKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            complement(6519..7721)
                     /locus_tag="KLF07_001459"
     CDS             complement(6519..7721)
                     /locus_tag="KLF07_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBD9831514.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFGGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            complement(7807..8631)
                     /locus_tag="KLF07_001460"
     CDS             complement(7807..8631)
                     /locus_tag="KLF07_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBD9831515.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKETNGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            complement(8782..8934)
                     /locus_tag="KLF07_001461"
     CDS             complement(8782..8934)
                     /locus_tag="KLF07_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon-specific DNA recombinase"
                     /protein_id="HBD9831516.1"
                     /translation="MARFRTGKPLVEFYQVSAFCGERVSSPGALPVNDPDYIMICPLN
                     PGSTPL"
     gene            8872..9135
                     /locus_tag="KLF07_001462"
                     /pseudo
     CDS             8872..9135
                     /locus_tag="KLF07_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9192..10385)
                     /gene="pilV"
                     /locus_tag="KLF07_001463"
     CDS             complement(9192..10385)
                     /gene="pilV"
                     /locus_tag="KLF07_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBD9831517.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNIN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGTILSCQSGSWRTIG
                     GKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPAGSRKWYKYEHKTGCIAS
                     CVTLN"
     gene            complement(10403..11029)
                     /locus_tag="KLF07_001464"
     CDS             complement(10403..11029)
                     /locus_tag="KLF07_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD9831518.1"
                     /translation="MLQMIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTVMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLRCNRQQRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            complement(11045..11530)
                     /locus_tag="KLF07_001465"
     CDS             complement(11045..11530)
                     /locus_tag="KLF07_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBD9831519.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            complement(11575..12111)
                     /locus_tag="KLF07_001466"
     CDS             complement(11575..12111)
                     /locus_tag="KLF07_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBD9831520.1"
                     /translation="MSVITCSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNQVD
                     PSTVCNADKTTIKLETNS"
     gene            complement(12173..13267)
                     /locus_tag="KLF07_001467"
     CDS             complement(12173..13267)
                     /locus_tag="KLF07_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBD9831521.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSELVIGYGLWLLAGLLLLAILIRMSFSLYKGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNSGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKSVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            complement(13269..14777)
                     /gene="tadA"
                     /locus_tag="KLF07_001468"
     CDS             complement(13269..14777)
                     /gene="tadA"
                     /locus_tag="KLF07_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBD9831522.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIRVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            complement(14880..15338)
                     /gene="pilP"
                     /locus_tag="KLF07_001469"
     CDS             complement(14880..15338)
                     /gene="pilP"
                     /locus_tag="KLF07_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBD9831523.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVQP
                     QITARIQLPGGRTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            complement(15328..16623)
                     /gene="pilO2"
                     /locus_tag="KLF07_001470"
     CDS             complement(15328..16623)
                     /gene="pilO2"
                     /locus_tag="KLF07_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBD9831524.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLESGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRDPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            complement(16644..18263)
                     /locus_tag="KLF07_001471"
     CDS             complement(16644..18263)
                     /locus_tag="KLF07_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBD9831525.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAD
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDAGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            complement(18295..18732)
                     /gene="pilM"
                     /locus_tag="KLF07_001472"
     CDS             complement(18295..18732)
                     /gene="pilM"
                     /locus_tag="KLF07_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBD9831526.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTHGRRISITLPAVIPDQVIIWMN"
     gene            complement(18736..19806)
                     /locus_tag="KLF07_001473"
     CDS             complement(18736..19806)
                     /locus_tag="KLF07_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HBD9831527.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTTLPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAVIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPPQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            complement(20033..20368)
                     /locus_tag="KLF07_001474"
     CDS             complement(20033..20368)
                     /locus_tag="KLF07_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831528.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KALQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            complement(20484..20714)
                     /locus_tag="KLF07_001475"
     CDS             complement(20484..20714)
                     /locus_tag="KLF07_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBD9831529.1"
                     /translation="MRCSVDAYNTCEKKVSYIPATEDINIAIMPFQTADNGLIIVCQN
                     SRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(20985..21650)
                     /locus_tag="KLF07_001476"
     CDS             complement(20985..21650)
                     /locus_tag="KLF07_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HBD9831530.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALAAC
                     RSLGWQIGTCAWSDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGYSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(21791..22432)
                     /locus_tag="KLF07_001477"
     CDS             complement(21791..22432)
                     /locus_tag="KLF07_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBD9831531.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            23135..24166
                     /locus_tag="KLF07_001478"
     CDS             23135..24166
                     /locus_tag="KLF07_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD9831532.1"
                     /translation="MAGLKNTSYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDTVWPVLVSFSDAVTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            24863..25021
                     /locus_tag="KLF07_001479"
     CDS             24863..25021
                     /locus_tag="KLF07_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831533.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            25076..25345
                     /locus_tag="KLF07_001480"
     CDS             25076..25345
                     /locus_tag="KLF07_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831534.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            25342..25623
                     /locus_tag="KLF07_001481"
     CDS             25342..25623
                     /locus_tag="KLF07_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD9831535.1"
                     /translation="MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            25663..26511
                     /locus_tag="KLF07_001482"
     CDS             25663..26511
                     /locus_tag="KLF07_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBD9831536.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            26628..27101
                     /locus_tag="KLF07_001483"
     CDS             26628..27101
                     /locus_tag="KLF07_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831537.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(27578..27844)
                     /locus_tag="KLF07_001484"
     CDS             complement(27578..27844)
                     /locus_tag="KLF07_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831538.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(27844..28338)
                     /locus_tag="KLF07_001485"
     CDS             complement(27844..28338)
                     /locus_tag="KLF07_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831539.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(28394..28996)
                     /locus_tag="KLF07_001486"
     CDS             complement(28394..28996)
                     /locus_tag="KLF07_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBD9831540.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            29350..30696
                     /locus_tag="KLF07_001487"
     CDS             29350..30696
                     /locus_tag="KLF07_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831541.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            30975..32855
                     /locus_tag="KLF07_001488"
     CDS             30975..32855
                     /locus_tag="KLF07_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HBD9831542.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(32873..33220)
                     /locus_tag="KLF07_001489"
     CDS             complement(32873..33220)
                     /locus_tag="KLF07_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HBD9831543.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
BASE COUNT         8153 a         7923 c         8628 g         8694 t
ORIGIN      
        1 acgatgaagt attatcagaa ggtgatgtgc tctgaacagg ttgctgcgga gtgggttcat
       61 cccggacagc cggttctata gtggctgttg tggcctgaag ttctgactca tctgcctgaa
      121 cgtggcctgt gtccggttgc gacggcatat cactgttttc ctgcagatgc tcaggatttt
      181 cattgttcat cagctcctga tatgcgctgt agtcttcgta ttcttcgggg ccatctgact
      241 caggaaggaa cggctctggc tgaaggggaa tgtcttcagt tacatggctg accgcctctt
      301 cccggggagt atccggggat attccggtgc tttgtttgtc atcaggctcc gtcatcacgg
      361 tcatttcctc tttgactgca tcaggggaag gtgatggctc ggctgattca ttcatggttt
      421 cagatgaggg actgtcttca ggggatgcgg tggtggcagc aggggccggt atttcaggtg
      481 aagtgccgga ttcctgttgt gactcgtttt tttccggttg tgacagttcg gcttcaaatt
      541 cacgccagtc tttatctgtg gccaccgaga caactttttc cggaggttcc gccggatgag
      601 cgggttcatc aggggcagac gccgttgtac tttccgggga cgccggtgat tctgcagggg
      661 agtcctgaac tacagagctt tccgtttgcg cctgaacgtt atcagcgtgc cgctcatctt
      721 tgccgttatt ttcagtggcg tgactggtaa atgactgtgt ttcatcatca atgttactgc
      781 tggaagcagc tggttgttcc tgctgcgtgg cggacggcag agaaggtccc tggtcatggc
      841 tgtgccctgt atcgggtgat gtttcaggat attctgtcgg agtcatggtt ttttcctcag
      901 tgtggaccgg tgtaaaaacg aggttgccta tcaccagtgc ctcgcgcatt tccggggtgt
      961 tactgttggg ggtggaaacc ccgatgtgtt ctaactgggc gttgatgaca tgctgaaaag
     1021 cggcccgtcc ccggcttgcg tccaggtcat tgaaatcggt cagcccctgc gctttttcgg
     1081 catcggtgaa cgcaggaaag atgaccgatc ctcctgtcag ttctgcggct tttgttgcac
     1141 tgacgatacc tttgttaacc tggcgtgcat gatcgttatc ggcacagaag ataaaggggc
     1201 tttgcttcca caattgccgg acattttctg ctatggaaat catgttgccg gcatccacgg
     1261 tcatcagcac cggaagcccg gttgcttcgt gcagggaggc cgcagtggcg tagccctcgg
     1321 caaacagtat tggctggccg ttgcgtggtg tacccagcgc aaaccagtta ccggttttct
     1381 cagaatcttt caggatgcgg gcatctttcc cgccagtgac cggaatacgc tggtaagagc
     1441 gtatcgcgcc gttacggtta ctgaagggaa taaccagttc attctggttg ttgattcgta
     1501 cacctggagc agcctgaatg ccttttcgtg tcaggtattc atgtgctgtg gcctgcggcc
     1561 atttgttgac atagcggcgg gcatacgctg cctgtctgtt gtactgtgct ttcagttccc
     1621 gttcagcgtc ttccttgcgt tgcagcgaat acgccttcag gtgaagcttt gcctgtggat
     1681 cggtttgttt accgccggaa aatacccatt tggtgggact gttatcgttg ctgcggtaat
     1741 cccgatacca gccagaaggg cgtccgtcca gaaaggcttt gtatgcacca cttttttctc
     1801 ctttccgatc ttctgttgtg gatacacggt gaattttgcc atccatgaca ggaagttcat
     1861 tcagtaccag tcctgcttct tccagtttct gggcaaattc gctgacggga tcagctgcat
     1921 ctgttgagat ttcgtgtggt cgtggacgcc atcggtccag taatgacaga tctgcacccg
     1981 ggcgggcaaa ccagagtttg tgctccttgt cccagatgac ggggctacgt ccttccatca
     2041 aaggatgtgc ttttttgaga tcttctattt catctgcagg cacggcaagc caggtgcgat
     2101 agcgggatac agacggcatg agtgcttctc ctgaaaaaag acggaaaaaa accaccgccg
     2161 gaagccgggg gtggctgggg ggatagaaaa tcgggggaga aatgtttgtc agggtctcca
     2221 gtctgtaccc ttattccagc gatcctgcca gtgttcaaga tattgtgcgg ctacatccgg
     2281 catattctta agcacaacga cattctctga attcttgcgt gaagcagcac gagagaagtt
     2341 ataggagccg gtttcaaccg tgtttccgtc aactatgatg actttgtcgt gatggatggg
     2401 gaaggcatct acagtccgga gaggaatgtc cagcaggttg atatatttca tcgcttcctg
     2461 acttgcccgg ttcgtattcc ccttgtcatc aacaacaata cggacgtcca ctccgcgttt
     2521 tttcgctttt gccagcgcat gcattacatc cgggtcggtg aaggaatatg ccatcatcct
     2581 gatggagctt tccgcactgt tgattgtgtc aagcaccaga gcggatgcgc ttccttcagg
     2641 agaaaagccg accttcacgg atggcgtgtc gatcattgca aacgatggtg tcgtgaacag
     2701 taacgggcat gccagtagcg atacccggag cagagtaacg atagtttttt tacctgacat
     2761 aaagactcca gaatttaatc gaggccagtc cagtctcgtg gtgattccgt atggtggcga
     2821 taccaccatg gacgaccgga ggcgatggcc cctctcagaa tgctgaaaat gcgagtacag
     2881 atcgtcctga cagtccagcc tgaacggtcg agaataacga aaaataaaat gatgccggtg
     2941 caaatccaga aggttgtttt ggagggaaag gggcatataa acagataaat catggtgaac
     3001 ggcagaggta cgccctggat gttgactggc ggtgttgcat atcgccagaa accttctctt
     3061 ttcatgaccg taactcccct ggcgggataa aaagaatcgc ttctcttcgg tcaacttcat
     3121 tccggatata catttccaga atctggtccc ggatacggcg cttctcctgc cggattatgg
     3181 catcaatgtg atgcccccac tctgcatgcg gcattccgga aagattgtcg cgaagctcat
     3241 cgttaatgac gatatattcc cggacggctt tacgaccacc gtcatttgtt ctgaccagaa
     3301 cctgaaccag aatgaactgc aacagtgaca ggacagccca ggccatcgaa tccctgatga
     3361 cagggggaaa cagcccaaga atacgtgcca gcgtttcacc aggagatttt gtatgcatgg
     3421 tgctcaggca gtaatgtccg gtgttgcctg cctgtaccgc tgcgtcggcg gtttcgttat
     3481 ccctgatctc accaacaccg ataatttcgg gattacgtcg tacagcagaa cgcagtccgg
     3541 cagcaaaact gaccacatca cgcccgattt ctgcctgatg cggtgggagc agatcgtttt
     3601 catttcccag gatatattcc accgggtctt catatgtcac gattttggcg ttgggataat
     3661 tcttcttaat gtaacagtac agtgctgcac acagcgttga ttttcctgac cctgtttcac
     3721 cacatacaaa tcccagaccg cttttgcaga ccattgcttc aagcaggtct ggttcaatgt
     3781 tcatggagag aatgtccggt atttctgtcg ggatgacacg catggttatt gagattgctt
     3841 tctcctccgc accagatgtg ccctgaatga ggtgagagcg taaccggatg cgttctccgc
     3901 gtttaagacc atagcgcccg gatgcatccc cgttaatctg tatcgtcctg tcaaccggat
     3961 tgcctgccag aacgcgggga atgacttccc gaccgaacag tgcatcaatc agggaggaca
     4021 tgagtgtggt tggtaacgtt gcactggaac aacgcacccg tcggccaaag cgactgacag
     4081 atacaggtga cccgccggtc agatccacat cactgacttt atgtgcggca caccagacaa
     4141 agaaatgccg tagttcatct gcagtgaaac gctgaaacgg aaaaagacca aactcatcag
     4201 gaatattcat cgggagcctc cttcagtgcc tataagcgga cgccagtgag atttctgaag
     4261 attgaattcc gcatgatttt taaggcgacg aacacgatct ccagtgacca gttttttccc
     4321 gtcacctgta acggtttgca tttgatcaga aacatccgga cgggtgatca tgccctgacg
     4381 ccagagaagt gagtacagca tcatgccccg gtaatcacgg gtgagggttt tctggttggc
     4441 ttcaagcgtc aggtcggcgt tttgtcttcc ttcctgccat ccctgccgta atgcggtttc
     4501 ccacactttt cgttgcacac tgtcttctgg aacgacgctg ccttctgtac ccggagtggc
     4561 tgttgttgaa agcccccgga gaagccagtc acgccagccc ggtggattgc tgacaaagcg
     4621 ttcagggcga atgatgctcc agacccggga agaggtccgg atttgatctg gcgtgatatg
     4681 agctacgtcc tgtgcttcat caataaccgg tggtagccag ccttccgggc taatcagtgg
     4741 ccggaaatcg tataaggcat taagtgtgct ttcccgggcg ttgagcgcct gaatcagctc
     4801 ccatgaacgc tgagccttgc caccacgaaa tccaagcgtt ttacctgcgt cggtcagcat
     4861 tttccagcgt gtttctgaca gactatccgg cttttcactt cgtggtttaa gccatgcgtt
     4921 aatgtcaggt ggtggcgtat tgacaccacc tggcgagatg gtaggggaag aagaacaacc
     4981 agtaagcagt attactggca aaatgagaga ggtgagtgct ttcatttttt ccggttctcc
     5041 gtctgcatga agctgacctg taacaggccc ggatattgat ggattgttgc tctccaggca
     5101 gtctgggctt caataagtct caacgtattg gaaaacgtca tatcccggac gtgaaggttg
     5161 actggtaacg gcaggcggac accattgtag ttaaactgca tccccctgac tctggccagt
     5221 tcgttcagta attcaattgc atcaccttcc cagtcaaagg aaaggcgatc gctgttagct
     5281 gtcagaatat gagaccccgt tgaaaccgga agttggccgg aaaacacacc tttgtcccat
     5341 aatgtgtact gagtgtgccg ggtaaccgcc acatcagagg cagcaccgta gttacgcgga
     5401 acaggagtat gctgagaagg ggagttgcag ccagccagaa aaagggcggc aagacacccc
     5461 gcaggtataa tacggttcat aataattcct gagcagttaa atcagaatgg gatataccgg
     5521 tgctccggta cagacaggat gatgtttgtt tccggttata cttgcgttac cggaaataaa
     5581 tgccagtgtg gccaggtaaa tagcctcata gtttactgaa ggtgattatt cctgatactg
     5641 gctggttgat gtgtcgtcag acataaaacg aatatgagcg taatctttaa cgctccgcgc
     5701 cgaatcgatc catgatggat gactcgacga tttatgaatc gaacgtaccg gcgcttgtcc
     5761 ctgaaacagt tcccggccct cgtccggata acctgcaatt cagacaggca catgtgcccg
     5821 tacactcttg tatcttactg agtatatcag aatttatgta aattcaaata tcctctatgc
     5881 ctctttgttg tattttccgt caagtatgag ccgggtgcca tcgtaatgca tgatcagggc
     5941 atcaaggtac gccggtttga atttcttgcc gagcctatct ctggccagaa atgataaaca
     6001 atgattatgt gtcattatta ctatgttcct tttatgagat tctcttgcga ttgattcaag
     6061 cgtcttataa agatcattat tgcagtctga taaactgtcc ataacgacgg gttctttacc
     6121 ggagaagaat tttgcagttt gtatggttct taccgcattg ctggagtaaa tatcatattc
     6181 actgaagatt gttgcgaact taacaccttc ctgctgtgct ttctctgtac cggtaatagt
     6241 gattccgctt ttatccgaat agcagggcat atctgaacgg tcgcaacgtt ctccatggcg
     6301 gaaaaggaaa ataacggtgt tatcagcatt aatttctttt gcctgttcca gggtgatatc
     6361 gggaagcgag ctttttgctt ttgtcactgc cgctaccagg aaaacagaaa ataaaagaat
     6421 gaatgctgtt tttgaataag aagaacgtgg tttcatcatg ttatttaaac tgaatataaa
     6481 aatacccccg ggtttagtgc cgggggcggg gacagtaatc agaaagtgaa ctgaagttgt
     6541 gccattgcac cgtaatcgtg gtcagttcct accaggccgg aaacatcgat atgtacgacg
     6601 tcaaatggag agataccaac gccagcggtg aaggcgcttc cggagtttga tgccatgttc
     6661 tgacgataac cggcacgcaa ttgcgcccat ttccatgcgt taaattcagc accaacactt
     6721 gcaaactgac gtttgctgtc agaagtgaag ccactggcag gagtcagatc aatgtcaaga
     6781 gcagtggtaa acaggtcatt gtgccaggat acaccggcgg ttgcctgcgg acgaactttg
     6841 aaggtttctt taaagccatt gacgacttta gtatcaatac tgcgtggaat gatgttctgg
     6901 gcaacaagac cgacagtcca gttgtcattc aggtctgtat aagcaccaat atcggcgttg
     6961 aatccgtttt tcgtgttgtg gtacttgtca ccgccaaaat cgtctttgtc gtaatcacga
     7021 accgttacgt tgtagttaaa caggtccaca cgctgatatt ttggtgtgac gccaagagac
     7081 catttttgtc cggccgtttc cagttctttg gcaaatgaaa ttccgacgtc ggtaattaca
     7141 gctgcacgcc cgaatgcgcg tgaggtcagg gcattcttat cgacgtcggt aattgttcca
     7201 ttggcaactt tgtccagata atccaggtct gcatcgttta cttttccgtt tacgctgaca
     7261 gttccgtaag acttgaccat aagggcaaac ggcagagtgt catttgccat ggctgctaca
     7321 gctgatacac ctacctgagc atctgcattg atattgcgga actcctgcag acgatgttta
     7381 agatttgccg ctgcctgttg tacgccatgc tggttatcta cagcactgtc aaacaaatcc
     7441 cagtcatctt taacatcatc agctttattt gatacatcat caggatcagc aacttgtgcc
     7501 ccaacagacg gcagaagaag gctgaaatca tcattgctgt tgtgctttgt cagtaaagcc
     7561 gggtttgcca gaggtgccac accataatgg gaagatgcta ccccggtgcc acccattgca
     7621 tcgttacgtg cttcaaaata tgacgtcgct gctgcagcgt taaaggaaaa taatacagca
     7681 gatgcaatga tggtgcgggt aatatgattt ttcttcatca tttaatttcc tgtttatcag
     7741 aatttagaca aaacaacgct atgacacgga tgtgccgtaa ttttttactt ttgtactatc
     7801 tggtatttac cagggtaata aagacgtatt aaataagcta acaggtagta tttgacctgt
     7861 aataaggttc ccggcaaggc caggtctcaa cgtgacgcgt cgcaaagaca ttgttgtatt
     7921 ctgcctggtt gtatattcag ttaaccattt tcttttttca tcgttcagaa gtgttggtga
     7981 taatgatagt gccggatata ttacagaacc atttgtttct tttttctgtc tcaatattac
     8041 cccgcaatat tctcttttcg tgtttcctgc gaaggggaaa ctatgccaga aaataacttc
     8101 agtgttgctg ctatcattaa taagcattct gaaattatca gcagtatctg gccattcacc
     8161 ggacaacgct atcggatagt tttcatggcc attcagccag tccggattga gattaaaaca
     8221 ttcagcaaga tgttcgagag cgtcagctga aagatagtct gcaagtctgg ccgggtcctg
     8281 tataaccgtg gagcggatat tccatggcga cagcaatgtt gccagtaatg gcgcatttat
     8341 atcatgcgac agcatgatgt attcaattct gcttattatg tttcctgtgg tgttatccgc
     8401 aggcataaac atattatgaa gagcatcctc aagtaggatt cgggttaaat cagcggtgga
     8461 tatggctaat tgttcgctca ttatccccag aacttctctt accccttgtg ggtaacggac
     8521 ctgcagaggt gtcgggttta tctgacgacc gttgatgagt ttttcgccga ttgctttttt
     8581 gtccagcgag agcatcattc gggccgcaat accggtcgta ttcagtctca ttgttcctcc
     8641 gactgaaaaa gtcttttcag ctcatgtggt tattgtatct tcggtgtttt ttctggtgaa
     8701 acgaataatt tcgatcatgc agatcgaatg tgtttggcga gttgttgcaa ctcacctttc
     8761 tgtatgtgtt ttgaatgatg tttacagcgg ggtgctgccc gggttcagag ggcaaatcat
     8821 aatgtagtca gggtcgttaa caggtaacgc tccgggagat gagacacgtt cgccacaaaa
     8881 ggcgctcacc tggtaaaact cgaccagtgg tttgccagtt cgaaaacgtg ccattgttgt
     8941 ggttacaaaa tgccggaaat gccacttcat aaacgcatct ggcgatgccc tgagtgtgga
     9001 gcagagcacg accgcgatat caatgcggca ctcaacatcc ggcaaaaagg aatactggaa
     9061 ctaaaggcgg cgggactcgt cgtctctacc catggaggcc agcgtaaatc cgtcgcacag
     9121 acggttgcgg cctgagaagt gggaagcctc gcccgacagg gcggggagca gtcactattt
     9181 ggatatcaaa cttaattgag cgttacacac gacgcaatac aacctgtctt atgctcgtat
     9241 ttgtaccatt tgcgtgaacc tgctgggctc tcaactacct ggcagtagtg ggcatcctct
     9301 gcgttgccca ttctggcaat ggcacagaag tcgaattgcc ccagataacc tgtggtggaa
     9361 agctgagtaa ctttgagttt tccacctatt gtccgccacg aaccggattg gcacgaaagt
     9421 attgtccctt tgctgtcgcg gcctacaagt ccatctggtg aacactttgt atttgctacg
     9481 gctgttttat ccagctgtaa atactcacct gtggaaaggc gaccgtcaga ccggactgtc
     9541 cccccactga cttttcctcc tttgatttcc cctgtggtta taattccttt accaccgact
     9601 gcgcgtatcc actggctgtc tgtcatcgta aaaccgccac cataggtgat gttcttccag
     9661 cctttatcat ttttggtgat taaccagccg ttttcgcttg taacatcacc tttcagtgtt
     9721 gcactttgtc cattgatgtt tcctgcattg ttcaggttgt tccctcccat attgatggcc
     9781 gtgttcatct ggttcagctc cggtcgtccc ggtacctgaa agcgatacag gcgatcgctt
     9841 tcctgcaggt catcacccat cattacgatg gcgatgtgcc cgccattgca gctgagtcca
     9901 tagtcgccgg gtttatctgt ccatccacca aaaacaccgg ttgctgtgcc attttttcct
     9961 ataaatccgc ccagaccggg gagcaggctg gcgatactgc gcagggcatc gtctttgata
    10021 ttctcccctc cggtggagca ggtcagggcc tccagacgac cggtactcgt attacgacgg
    10081 atgccggtgg tataactctg cccaaagggc gagcggttaa gacccttcag atatcctttc
    10141 cggattaact catccgccgt gaaggtggca ggaagcgttc cactatgcag gcgggcattg
    10201 ttatcggcga tgtaggtcct ggctgcagta ttgtaggcat ctgcatgaaa tgcggcgctc
    10261 tggttgcgca tttcattcag gtagtcttca atgtaagacc agaacagcgg catgagcagg
    10321 ctgatgacaa ccagtccccc cagaatttca agaagtgcaa aacctttatc gttgtctttt
    10381 tgcatagtcg ttccctccgg tattaccaaa ggccaccggg ataaaaagta aatgcgtact
    10441 gtgatgcgca gagaaacggc ccaaagggaa gttctctggt aatgcgttgt tgtctgttac
    10501 accgcagata aagtaaggct ccgcctgctg ccgtcgcgac aatccagggg atgtctcccg
    10561 gcggggacca gatggcaata ccggcaatca gaaaaacatc gcccaggcct atggtttctg
    10621 ttttcctgcg gtaagaggtg tattgcctga acagaagcaa cagcaggaac cagaaccctg
    10681 cactgcacag gcctgtcagc cagtctccct gccagagacg aaatgcgctg ccgcttatca
    10741 gatacatgac ggtgaagggc cggggaagac ggcaggatgc agcatccgtc agggtcatca
    10801 gtgtcataca tccggtcatt atcgtcataa acagcacccc gacagcgctg tcattctgat
    10861 aaagacgcag gataatggcg gcagctgcaa gcatcccgct catcagcgcc agcccatggt
    10921 gaggggggcg ggcattcatc cgggagagtc gatagcgact catctgaacg gccagacact
    10981 gtattaccac ggttatggcg accagcagtg ccgggatcat ctggagcatg gcagttctcc
    11041 cggactaacg tccctgcatg taagcgctgt aaatctgccg ggcgtatttc atccggcggt
    11101 gagtgttact tttactgaag ccggcgttat aactgccgag gcattcccag tttacgccgc
    11161 atcgctgaaa atgccgggcg aggatccatg ctccggtctg aatattcagg cagacattat
    11221 tcaacagatc tttctcactc cggataatgc ccattttttt cagcccggga atatgtgtgg
    11281 agttaatctg cattatcccg taatcggtac tgataatttt tcctctgtca ttcctgttat
    11341 gattaatggc ccggggattc agactgcttt ccctgcgggc aataattttg agtaatcctg
    11401 gatcaatgga ataacgtttt ccggcggtaa taaaacaacg atcatattgt ttgtcctgtg
    11461 gggatgcctg tgcggaagga agtgagcata acagtgaaaa aatcactgtc aggataaata
    11521 acctgttcat acagtgacct taaataaagc gcccgggcgg gcgctgatga atgattaact
    11581 gttggtttcc agtttgatag tagttttatc tgcgttacat actgtagacg ggtcaacttg
    11641 attcgtcaca ttgccgttga tttttgtaaa catggatgaa ctgcgcaggc tggtgaccag
    11701 atcaatacag tttttcttcg gcacattgct ggattcaata acgtaggagt atttatctga
    11761 actgactttg atggttaccg caccacccca ggcatttttc gccttattac caccggcgat
    11821 catatcggca ggcaggccac cactttcggt caggattttg acgtagtcag tacctgtata
    11881 ctgcccctgg aatttctggg ctttcatttt ggtggcgatt gtctgcaggt ttttgagttc
    11941 cttctggttg tctgaagagg acagggtatc ggacataagt cccatcacgg caccaataac
    12001 aatggcagcg acgaccagaa caccgaggac ttccagcagg gtcatgcctt tatcatcgtt
    12061 ctgaaggtga cgaatattat gttctgaata cgaacaggtt atgacgctca tattagctcc
    12121 tgagattggt aaaacagaat ttggatcgag gggaatataa aggatgctgt gtttactgct
    12181 tcagcatgtc actgatgccg taaatggcac tgaccatatt gatgatgaag agaaatacca
    12241 gcagacctga tatagcagta ataataatgc cgctttttct gacactcttt tttgcttctt
    12301 ccagccagcg gtctgcataa agcgccatac tggcaacact gtcctccctt tctgaattta
    12361 aaagaagatt attcacacag gattttgacg gaaagtcata accactgttt ttcatggcca
    12421 gtgccagtga ctgaccggat aagacatttt ttctgatagc acttattcgt gattttaacc
    12481 atcggttatg ttctgcatgt ttttctattc tggtaagggc atgagataaa ggaatattta
    12541 tctgcagcat ggatgaaata ttaaaaaggt aattcacgcc atggaatgtc tgatacaggg
    12601 accacggcgg gatgcgatca agataccgtc gtcggggacc tttatacaga ctgaatgaca
    12661 tcctgatgag aattgccagc agaagcagtc cagccagcag ccacaaacca taaccgataa
    12721 ccagctcgga ggatttggcc agaatgacca tctgcactga ccagcgttct tccggtgcaa
    12781 aggcgctgat catcggaata acccgggtat gaacgttata catattcacc acacagagcg
    12841 tcagcaggat gagcggatag aggcagacac tcaccagact gctaatcatc tcctgtcgtc
    12901 cttttgccgt tttgacaacc tgaaacatgg cttcagcgat atcaccgcgc tcttcaccgt
    12961 tacggatcag ggcaatttca tctttgggta cccagtcctt cagggccgga gagagctgac
    13021 cactgctgcg tatgctggcg atacagtctg tcgccagaaa atacaggccg gactgccctt
    13081 tctttttacc aaaatccgtt tccacatcgc agatgaactc cagtgcccgc ataaccggtg
    13141 tgccgtcccg gatcatgcct gcaagggact cgtaaaacgt agtccgcctg aacgtctttt
    13201 tgcccagcca gcggttaagg ccggacagtt ccagttccgt attgttgagt ctttccagaa
    13261 ttttcatatc agtgcagcag cgtatagctg tcctcgtcta caggggatat gtaatgcgca
    13321 gccagcggat cgacctgacc actgttgatt ttgcccagca ggtgctgatt tcgtgtcata
    13381 ccgccgagct cccggtgcca gtaggttttt gcctcaatcc ggcctttttc ccggtaaatc
    13441 tggaaaaact tcgcatccgg ggaaatcact tctgcaatga cggtccggcc gttcactcca
    13501 cgccagcagt gtgggcagcc gttatggtta cgaagataca cggcatcggg ctggcagaag
    13561 ttttccacca ggcgtcgttc ttcatccgtc cgggagctct ccacctcatg ccaggggagg
    13621 cggcagtggg gacatatcac ctgaaccaga cgctggctga gcaggccgat aaataactgc
    13681 ggatctgcaa tcatccttgc ctgaacgccc tccatttcca gtcgctcaag aatatttatc
    13741 gggtcgttag catgaagggt ggtcagcatc aggtgaccgg tcatggcggc ttttatcgcc
    13801 gtgatggcgg aggcatggtc acgtatctcc ccgttcagga tagcgtcagg atcaaggcgc
    13861 agggcggact taacagaatc gacccagccc tgagcggtat ccataacggc cgtctgaatt
    13921 gcaccgggga tacgcccctc tggtggtgat tctattgtga acagacgtcg ccgggggagc
    13981 aggatccctc cggtatcgtt gaagccatac tgttccagat aggcggcaga agccgtcctc
    14041 agcgtggtgg attttccgga accggtgggg ccagagagga tcacgatgcc ctccggtcgc
    14101 tgcagcatcc ttcggacagc gcgctcctgc tcagggtgat accccagcat actgaatgtc
    14161 ggtatatgct gactgtcatc cttgatcaga cgcagtacgg catagagtcc gcccacacac
    14221 ggatagtggg agtagcgcgc accaaacagt ccggccagtt tcaggaatct ttcagccaga
    14281 cgggcatcct gggggacacc gggatcaaac tgtgttccct ttatgacgtc agtcattccg
    14341 gaataggtgg cccccagtaa ttccagcccc tcatcttttc tgacgatatc gagcagctcc
    14401 agctctccgt ggacacgggc ctccagatac gtgaaatccg tactgtcacg ccccggcgtg
    14461 atgtgaagat ctgaggcgct gagggtggcc gcatggctga tatacccgag cattttttgc
    14521 tgtacggggt taattccgtt ttccaccccg gaaatgaccg tctcctgcct gagtcgggcg
    14581 atcacatcaa gtgtggtgaa ctcaaacggg gatactttac ccggatagcg ggagcacagg
    14641 cgggtcagat aatcctggac ggcacgaagg tgtttttttc cttccaccac tctgacacga
    14701 atttgtcctg ttttatctgt atcaacaacc acgtaatcca gaatgtcatc aggtggctgg
    14761 tctgcaggtg ccagcataaa atctccttac gggcagcggt acccggaaac gatttttttg
    14821 tgattaaggg ccgggggaat aatctgttcc gacagccgcc tgtaaatggc ggcactgagt
    14881 cagggttgta gtgttgttct gataccattt tgttcaagga caacactgtc agtggtgatc
    14941 ttttccacgc ggaatgtggt gccgggaatg tgttgccctt cttttacatc agcagttcgt
    15001 ccgcccggca gttgtatcct ggcggttatc tggggctgaa ctccggatat ctctgtcagc
    15061 cggggaatac cggtaatggg ggcaggggta tctgtcgtga cggctgaact gctttcctgt
    15121 gaatgcacag gctcagctgg ttctccctgc tccagggttc tgagttcatg tcgcaccttt
    15181 tctcttgcca gcatggcttc atacagtgca gtttcttcct gaatctgttc cacctggagt
    15241 tgccggttat tctgttgcat gttgctgatg gtgctggccg cgaccgttgt cggcaggatc
    15301 aggacggaaa gcagaagagc ttttgtttta ctggacataa tatttacctg tgaattgata
    15361 acggaaacga ccctcttcca gttgccttgt tacggagata aaacggacgg agggttcact
    15421 gaaatgctct gccacctggt gcgccggaag tgtcgtcagt atctgcaggt catactcctt
    15481 ccaggggggc tggatggtat ttccgtcttc atcggtgaag ctgttgctga cttcctgcca
    15541 gtccattttc agctgcatcg accgggcgag tgtggtcagc cgttcctgca ggagggtgtt
    15601 ttccggtaat gcctcaacgg caacgggagt ttcaggcata ctgaaggtga ccggcagggc
    15661 aaaaccaccg ctattcccgt ccggcataac cataaatggc cggatgctga aaatttccct
    15721 gacccgctgg ctgaatgcgg tcactgtcgt cccggcgatg tttgtatatg atgcacgcag
    15781 attgccgctg ttcccggtgg cagcacactc ggcgagtgaa tatcgccagc cggcgactga
    15841 caccggcagg cgtgaccata cgtcactgca tccccggata aaatctccgg tttccggcat
    15901 actcagccac ggtggtgtga tccgttcctc tgcggctttt tgtgctgctt cagcaagtgc
    15961 tgctttttct gccttcaggc gttcatagta ctgccagcca taccatgcag tacccagaag
    16021 ggtgagtatg agtaataccg gaataagctg tggcccacgg gatacggggt gaaggcgtgc
    16081 ctctgccgga tatttccctg cacctgtcag ggtttccagt ggcaggggat ctctgccgtc
    16141 aggtccgtta aacgagggcg gttcataaca tgtccagccg tgttcaggcg cgctgttaat
    16201 ggtgaggaat atttcccggg cctccctgat ttcctcccgg gttcccacca ggtcactgac
    16261 caggatgccg tcaacagcag aaacaaaacc ataaagttct gtatcgtttg cctgtgacag
    16321 ttcaacgata gcgtatcctc ctgactcgag caggggcagg ataagcatgg cgagagagaa
    16381 atacctgcgg ctgcctttgg ggagtgtatt ctggctgacg gctgcaacca tgatgtgacg
    16441 atcggtatct tccgtactga gatactcaca ggaaatatag cgtactgccc ccagactgcg
    16501 ggcaaacgtc cgggggtttt tatggcttgc gggcagtgtt ttccagtcca ttccggcaag
    16561 aaatacattc cgtcctgccg tcagcggaaa cacctggcgc tctgtcggtg aaatatccgt
    16621 cacgatagtc ctccttctgc aaatcagatg atattgggcg tgatcagcac cacgagaatg
    16681 gtgtcgtcgt tttcactgtt gatcccaccg ccaagaccga agaatcccgg tgtgccgaca
    16741 ccctgttttc cactgcgacg ccctgactgc tggaagccac tgataatgac tgtctgcccg
    16801 gatttcaggt ccactgtctg gttgatggtt ttcagatcca cattcggcag ctgggctttg
    16861 cttccaccac tctcgtaatt ctcaataacc ggtttgtcgg agagactcat ggaatagagc
    16921 agttgcagtg tctgtgagtc cgggagaatg aacggcagaa gggtcatatt aaaaccggtg
    16981 gtgatggtgg ccgcattcag ggaggtggat gagccgacat tggcggtggt atccgtcgtc
    17041 acactctcaa tgtagctctg ctgattggca atctgcatcg gtaccggggt caggtttttg
    17101 gtcacggcgg aattccgggt taccacactg acatctccct gggaagagag tgctttcagg
    17161 aaggcttttg aaccggtgag tttcccgttc acaatggaaa caccaccagt gacggtatcg
    17221 ctggctgcac tggtgaatgt gctgccgagg ctcagtccca gcgttctgtc gctgaagact
    17281 gcgttccagt cgatacctgc ctgctcatta cgggttttcc ggatactcag aatttccaca
    17341 ttcagcacca cctggcgctt catttcttca ttgcgttttt caatgaacgt ccggaccgtt
    17401 tccagtacct gtggggtgtc cgtaacggtg ataaagcccg tcgacatgaa catgcgacca
    17461 atccccggcg tcagcattga tgagacagca tttttcacat cctgatacag cgcagatttc
    17521 atatccacgg tggtggtctg ggtattactg gcatcaccgg aaatggcatc cgatgctgtt
    17581 ccacctgatg agccatttga tgacatggta ccgctgacca cggtggcatt ataggcaacc
    17641 tggctgtcca tataggtcac cgggaaggtc cgggtatcaa gataatagaa cgtgatatgt
    17701 gaaccatcat aacgccagga aatcccgaga cgggaagcga ccgcattcag cagtgatgtc
    17761 agtcctgaag ccttcagttc aggaagtgac gccgtatttc cctgcaggcg gggaagtgtc
    17821 gtgtcagaac tgcctatggc ggccagtgga accataccgc cggcatccgg cggtggtact
    17881 gtgccctgga tttgctgcgt actgccggag gtggttacgc tcagatacgc ctgtacatca
    17941 ggggtgatcc ttacaggaat atgacagttt tccgagatcc gctgggccac ctgctgaagg
    18001 gtgatgctct gccgggtgtt aatgataatc ggacagccgg gcaccgcatt accggggttt
    18061 ttatccgtga ccggacgggg attgatccac tgacgtgggg attcctttac aaccggtgtc
    18121 cggtttcgca gatcagcgat catttcccgg gcccgttgtt gttcagcaac ggcgtctttt
    18181 ctggtgttct ctgcctgtcg gaaactggta cagccctgca gcagagtggc tgcaatcata
    18241 atagcaagaa gttttttgtt catagataaa tccgtgagtt attcagagtc cggcctaatt
    18301 catccagatg atgacctgat cggggatcac agcagggagc gtgatggaaa tacgccgccc
    18361 atgggtgtcc aggaggcgac cgttttccac tctggcaagc agggccgaat caccactttg
    18421 ttccaggagg gcacccataa ggccgggttt ttcttttgcc cagacaaaaa cgcgctgctg
    18481 actgatgagg tggctgacgt ttttcgtggc gggcagaccg agctgtgctg acgtgagctg
    18541 accaccggca gcacgtcgtt cagggtgctg atacaggtaa tcgttgatgg cattcatata
    18601 acgaataaaa tctgcagcct gctgtgtgat ttcagcggtc tgctgatggt gcacagtacg
    18661 gctgaggtga ctgtggaacc agaaattacc aatggccata atgaccataa tgaaacccgt
    18721 aacccatccc ataggttatt cctgcgggcg gtctgtcaca cggagcaggc attgtgcagt
    18781 gctggtccgg gcatacagcg gtttttcagc cggggtatag agtgcaaaga gctgctccag
    18841 cgcctggcgg aaactgccgg tgaaaaccag aggggcatcg atgctgtagt tgacaggtgt
    18901 ttcccagcgc agtgtccatg tgccgtgcgg gcatttttcg gatgatgtcc agcgggccag
    18961 tccgtctttc agggtggtcc ccttttccat tttccactgc cgtggcggta tgaccttctg
    19021 cggtggtggc actaccggtt tcgtggccac cggggctggc gtggttgttc tgctctctgg
    19081 agttccggat gtcccggtgg tggtctgagc ccgggaaacg ctgacggcag gtgtcactgg
    19141 tgtcgggcct gccggcgttg ctgtgctgcc cctgaacgga ttacgcggtg tatcagcagc
    19201 tttctgtgaa ccctgcggtg ccgtgacgtc gaccggcggt gttgcagagg tcgtgacagt
    19261 cagtattttc ctgttccagt ccatatgtcc gtaaagctgc tggccggcca gcagcgcatt
    19321 cagtgcccgg tgccactggt cattaacgga ccagacgggg aggcgtttgt tcagtgccgg
    19381 tatgacagcg ttctgttgcc ggaactgcca gccgggtggt agcaactggc gtatccagtc
    19441 actgacggag cgctgatgtg cctcacgtaa ataaacgacg gatgactgac cgggtttacc
    19501 ggtcataacc agtgcgggca gctgtgtggc ctgaaggagc ggacggggag gcagtgtggt
    19561 actctggctg ctgactgctg ttgtgggcgt cgctgttgtt gtcgggcggg gcggcgtcac
    19621 agggcgggtc accggtggcg gaagcagacg tttttgcgta tgggcgataa tggcagcgct
    19681 ttcgcgaatt tgtccgtcga cgaacaccag agccggaggc gtggtgtcag gaacaggcgt
    19741 ggcattaaat gccgtacagc cgctgagtaa tgacggcaga aggagtgtaa gtgatttctt
    19801 tttcatagaa taaatccggt atgtatcggg ttacgatata agacaggtgg ctggtaacag
    19861 gtcttcagat aaatataatt tcatcaggat attgatgatg aacattcata ttcggggtga
    19921 ttatgaaaac taaaacattc cggttccgga aatataaaaa gaaaaacgtc ctgctgcaaa
    19981 tgatagtttc cagaaaacaa gtgataatgt ttttcagaac tggaacatta ttttatttct
    20041 tgcctgttat tgaaacatgt ccggttatga tctccgaatg gcaggtatca ataaaatcac
    20101 ttttccccga ttgtgtgtca aagtcaacgt gggtgaatct gtgtacatcc tgccatatgg
    20161 tgaaacagac aggacaggta acaggtagtt taccttcagc tattttttcg cagctttcaa
    20221 ccagttgcag cgccttgttg tatctgacgt tatagccatt tgtgcaaagc gtttcatacc
    20281 catgagctcc tgtaactata tgtgtggcga ttctttcgtg gactctccca ttaattaata
    20341 attgttcacg tgttgtgatt gttctcatgc ctgtttctcc attagtcata acagaaaata
    20401 atctgcattc ctgaaaaata tcgcggttgt gaaatattta atttttaccc ccttttatgg
    20461 gggatgaaaa gcaggctgtc aggctataac tgaacgcggt ttctggattt tggtgctaat
    20521 gtcacatctt tccattcaca gctgcttccg gggatacgct ctttgcgtcg tattatacgg
    20581 gagttctgac acacaataat taagccattg tctgccgtct gaaatggcat tatagcgatg
    20641 tttatatctt ctgttgcagg aatataactg actttctttt cacatgtatt gtaagcgtca
    20701 acggagcacc gtattgacgc ttactcctgt ggcaggtcag tcaaacaaca ttatggcgtg
    20761 aagcttccgc ttctttaatt gtcagaagaa gcatatcgtc agtcatcaga agacaagggg
    20821 atgtctgcag gaggggatga taacaacaga agcctcacgc cggaatgcgc tttatggcaa
    20881 ccggagagga cggataatca aaaatgggac atccacatca gcaaaccgcg atttgcgaaa
    20941 aaaagtcagc ggagagtggc tgccgttgtg gctgtcccgg tatttcagtg taacaggtga
    21001 ttgctgtgat ctatgcccca ttcggctttc ggtaaacctg aataccccac atgaagaatg
    21061 cgtgacagca tgtttctgcc gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata
    21121 aaaccttcat cccggaatat ctcttccact tctgaagatt cgagggatgc cggatgaatt
    21181 aatgaaaatg ccaccggtct gagcattctg cccgtggtgg aaaaggtgtc tctggtactg
    21241 ttcagttgca ggatatgacg tttacctgtt tgcgcacggg aaagtgtctc actcagcagc
    21301 tggtcgctcc atgcgcacgt gccaatctgc catcccagac tcctgcacgc ggcaagagcc
    21361 gagcggaagc ggtacaggtc gtataatgct gcgttattgc cactttccag cagtgatttc
    21421 agggtgtttg ctaccggtat gtctgaattg gtggccagca gttttcgcag ggtagtgaac
    21481 tcttcagcca ttcctctgaa ctcgcgcatc tgacgggcag tgtcaagcca gttcagcagg
    21541 taacgccggc gacgccgttc gttgtttatg ttgtccgggg cggtggagag tgcttcgagc
    21601 atattctcca gtaacagaat gacaggattt tcctgggata gaatggtcat aaaggcgtcc
    21661 tgaaaaaatg cccgacagcc gctctccgta caccctcagg tgtcaccggt gccgggcatt
    21721 gtggaaacag aagggaaaaa aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag
    21781 acagcaattt tcatgtcaac gctccgtcat cagataccag gtgcaggagc agggcgatcc
    21841 tcgactccgg cagaggttgt gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca
    21901 gccagagggt ggaggcagca tccagctcct ccctggctgc cggattgagc gcaggatccg
    21961 ggtacacttt catcaaagac tgaacgatat cctccctgac ggttttgatt tcaccggcca
    22021 ttttcacgaa gtccagaaag gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg
    22081 ggtctgcttt caggaaaaag taaccgggaa acacggcgtg tatatgtcga cggtaaccgc
    22141 agactttatc cgtacggcgg acttttttca gtatcagggg tgtccaggga aggacgtgtt
    22201 gttcggaaag ccaggaaaat agatgttcgc ggtttttgcc ggcagggatg tactgagcca
    22261 gataccagtt gcgttgatgt aattcactga tattcaaaat ccggaacgct ccgcgaaaat
    22321 cgatccccgc agaggggctt cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg
    22381 gaaacagcgg ctgacccacg ttcagcctgg cctgcagttc tgacaggcac atgatttacg
    22441 cactcttgta agccttgtgg acggtttcga ggcgggcgaa aaccggcaac tcctgcaaac
    22501 aggattaatg gctggcaaat atattacata aggatttatc tgtggcaaga gtgaagatag
    22561 gcagtttaat agatcgttat atttgataag tcagcatatt attctgattt gtgtgcggtg
    22621 tctggaggat tagatcgttt aaaaatttta tgcgatctct tctactaaaa atgatgaaaa
    22681 acacagcata aaagatcgtt tgtgaaattg ttcatttttt tgacaaagtt caaaaaaacg
    22741 acgcttttta gggggttaaa gagggcaact gtaaccctga gaaatcgtgt cggcttttca
    22801 aagcggtgga aaaggggtat attgcggatc gttatgcagt ggcttttggg atcctcgcgg
    22861 ttcggaaagc cagaaaacgg cagaatgcgc cataaggcat tcaggatgta tggcagaaac
    22921 gacggcagtt tgccggggcc ggaaggctga aaaaagtttc agaaggccat aaaggaaaac
    22981 ccccactatc tttcctcgaa ctttggcggg ctcgtgaaag atagtagggg cgttcacaga
    23041 atacgggata agtatatatg aaaccgtacc agagattcaa ccctgtgcag tgtataaata
    23101 cacggcacaa tcgctccgcc ataagcgaca gcttgtggca ggtctgaaga atacttcata
    23161 taacgcagta cactggagtc agttagcacc cgaagagcag atccgtttct gggaagacta
    23221 tgaagcggga agggcgacca ctttcctggt tgaaccggaa aggaagcgca cgaagcgccg
    23281 tcgcggtgag cactccacca aacccaaatg cgaaaatccg tcctggtatc gtccggagcg
    23341 ctataaggcg ctgagcgggc agctcgggca cgcctacaac cgtctggtga aaaaggaccc
    23401 ggtgaccggc gagcagagcc tgcgcatgca catgtctctg catccttttt acgtgcagaa
    23461 acgaacgtat gctggtcgca aatatgcttt ccgtccggaa aaacaacgcc tcctcgatac
    23521 cgtctggccg gttctggtca gcttcagtga tgcggtcaca cataccgtag gcatgagtgt
    23581 ttcccgtctg gccagagaaa tcagcccgaa agacagcaag gggaaggtta ttccggaact
    23641 ggaagtgacg gtctcccgcc tttcccgttt gctggccgaa caggtacgtt ttggtgtgct
    23701 gggtgtttca gaggaaaccc tgtgggaccg tgaaacccgc cagcgtctgc cacgttacgt
    23761 ctggataaca ccggcaggct ggcagatgct gggcgtcgac atggtgaaac ttcacgaaca
    23821 gcagcagaag cggttgcgtg aaagtgaaat ccgccagcaa ctcatccggg aaggcgtact
    23881 gcgtgaggat gaagatatct ccgtacatgc ggccagaaaa cgctggtatc tgcagcgcag
    23941 tcaggatgca ctgaaacacc gtcgtgcgaa agcagcagcc agtaagcgcg ccagacgcct
    24001 gaagaaactg cctgccgacc agcagattca tgagatggca gagtatctca ggaagcgtct
    24061 gccgccggat gaagcctatt tttgttccga tgaccatctg aagcgactgg ccatcaggga
    24121 gttgcgtcag cttgaactga cgctggctgc cccgccaccg cactagacag taccattccc
    24181 tcagcactga atcatcgcca gcccctccgg ggctttcggc gctggttccg ctcagcccaa
    24241 aatccgcagt aatcatctta aatcccctca gaggggcata tctgtccata aaaccacgca
    24301 tcagtcatca gaacgtggcc acgttgtttc agttatccac ataaatccgc aaacaaagaa
    24361 ctttgagaag ctgcaagcct aaaacagcaa acctgcaata tagtcttaac cccattattt
    24421 aatcccctgc gttgcttcgc cgcagggaaa gtctttatct ctgaaaccac tgtgaataaa
    24481 tacaaagagg ccttcgcttg cagcggccaa ggccgcgccg ctcagaatct aaaagcacct
    24541 cccacgctga cgcgcgggcc ccggaacctc accgttctga gaccacagca aaaaaaaatc
    24601 aggaataaaa acaccacata aatgcagcac cgtgcccacc cctcataact gaaaagcgag
    24661 gccgcccccg cccaaagggc gggaacaaca tcgcttttaa ttatgaatgt tgtaactaca
    24721 ttatcatcgc tgtcagtctt ctggctggat gttctcagta cacgctcgta agcggccctg
    24781 acggcccgct aacgcggaga tacgccccga cttcgggtaa accctcgtcg ggaccactcc
    24841 gaccgcgcac agaaactgtc tcatggctga aagcgggtat ggcttagcag ggctggggat
    24901 gggtaaggtg aaatctatca atcagtaccg gctgacgccg ggcttcggcg gttttactcc
    24961 ggtatcacat gtaaccacag cgtgccgcct tccatgccgc tggcgcggca tatcctggtg
    25021 acgatatctg aatcgttata tactgtgtat atacgtagta atgacgaggt gataaatggc
    25081 acaggttaat atgagtttaa gaatcgacgc tgaactgaag gatgctttta tggccgctgc
    25141 aaaaagcatg gaccgtaatg gctctcagtt aattcgggat tttatgcgcc agaccgttga
    25201 acggcagcat aatacctggt tccgtgacca ggttgaggca ggacgtcagc aactcgagcg
    25261 cggcgatgtg cttccccatg acatggtcga atcttccgct gctgcatggc gggatgaaat
    25321 gagcaggaag gttgccggta aatgatggag atattctgga ccatgctggc cagccaggac
    25381 aggaagcgca ttcgtaagta cattgcggag cagaacctga tggccgccat tgaactggac
    25441 gaacggattg gttattcagc cagcagcctg gccgggcagc cgtataaagg ccgcaacggt
    25501 cgtgtggaag gtacccggga actggttatt cacccgcatt ttgttctggt ttatgaggtc
    25561 gacagccagt ggggaaaagt gtatatcctg cgcgtgttgc atactgcaca gaagtggcca
    25621 tagaggggct gtccggatat aaggaaagga gatgcaataa ccatgaataa tgatgagctg
    25681 gccaccagac gtgcgcaggc gattgccgaa gacaggtgtt tctcgaaagg acgtttgcgc
    25741 gatgaattca gaatgaaacc agcccccggt gctgagccgg ttaagtggta taaaaacact
    25801 tatggtggca ggttcgctgt ataccgaatt gcagactgtg ttccgatgcg tgagaaacgc
    25861 cctctgacga gtaaacaaca gctggctggc cagcgacttt ccgttttatc cagacttaac
    25921 agcaccagtg gccggatggc acggcaggca tatgactggc tgagtctggc accgctcttt
    25981 ctggatactg aaacgacagg actggataat accgcggaag ccctggaaat cggcctgacc
    26041 gatgcggccg gtcaggtggt atttgaaacc aggctgaagc ccacggtcgc catcggggcg
    26101 caggccgccg ctgtgcatgg tatcagcgag caggctctct gcggcgcacc atcatggaca
    26161 gatgtggcgc ggcaactgcg tcatgcaatc ggggaccgac cagtaattat ctttaatgcc
    26221 cggttcgaca tccgcattct gaaacagact gctgccgcac atagcgatcc ggctgactgg
    26281 ctggaagaac tgacggtata ttgtgcgatg gagctggctg caggatatta tggggccacc
    26341 aaccgctatg gcacgatttc actggcctgt gctgccagtc aggccggact gacctgggaa
    26401 gggcaggcac actcagcgat cgccgacgca cggatgacgg caggggtggt aaacgctatt
    26461 gctgcatatc atctggaact gctgcaggaa caggcacggc tgaaaatctg accgcctggc
    26521 ctgtataccg caatcattta cgttatccag acgtaaatcc gccgcttgtt gctgtcagca
    26581 tgttaatgct aatgtatgtg tacattagca ttaatgaggg atgatatgtg agagacaaaa
    26641 ccagggaggc catgcggctt tttttaggcg gcaggtgcta cacggctgaa aaacttgaaa
    26701 aggattatct ggctgaagtg gctaattaca gtaacgaccg ctgggaagca ccgcaacgtg
    26761 cctcacgcct ggctgccagc gtaaagcgtt acaagacatc agaaatgctg cgctttattt
    26821 ttgctaccat tgcctacgat cccgatccag atttaacccc gctaactgtg agacgtttgt
    26881 gcaaggcatt gttcggcaga accggaagcc agtggctggt tgttgaagtt tttggggaga
    26941 aggggcgtca gcaccgcagc gcggacagca atccggagat ggtggaaaaa atggctgcca
    27001 gatatcgtca tgccgccgaa ctacactggt ccgcaacgct ggcagaaatt gaacgggtaa
    27061 agcggcttta tcagacgaaa attaagaaat ccaaaaaata agtgggttga ctgccattgt
    27121 tgttcttctc cttcatcatt aatgttaatg tatgcataca ttagcattaa tgatggtgcg
    27181 ttaaagtatc actggtataa tctggtaaag aacatattga gaatgagaac ttatcatgtc
    27241 gtcacgcaac tctgtacatg attttataat gctgctcccg gcgctgctgt tggttgggtg
    27301 gttagttttg gtgataattc tcatgtttag ggatgataaa tgtgacaaaa cttaccgtga
    27361 aggttcgttt cccgaattta tttgtctcca ttctgatcgc tcgtcacttc gagaaaccga
    27421 ttctgagaga ctcattgtca aatatacaga aataaaatca caaaaatagg taaaaccaat
    27481 aatactatag ctaaaaaata gttacttttc tgtatcaccc tgtctctctc tgctttttca
    27541 tggctactgt catatttatg tctgttgcgc ggttttgtta ctttttcatg accgaacccg
    27601 aaattttgcg cgtcaataag agacgttgtg taaactcagt ccttttatct ggcaggttaa
    27661 cccctatcaa tcttaggatg aaaccacgcc atcctctgtt tgcctggtta ttaatttcat
    27721 aaagacgatt gttttcctct cgtagttttg tccactctaa acaaagtgtt atatcatcca
    27781 gttctgtgat gtgtttcact tttccattga cagttacagt gtttattcca tttcctgtga
    27841 gcatcatctt atgccttttt tatcttcatt tgggaccgtg cttttcttcg gagtatcact
    27901 attcgttaac tgctgtttat caataaaatt cctgatgtta tcaagtgctt cctttgtttt
    27961 tagaatgcgc ttgatatcca tttcatcccg cccggtcatt ctttttccgg acaaaagacg
    28021 ctcaagaagc tcctgtttcg catcaggaga atctttggcg agatccatca ggttaagcaa
    28081 atcttcacga tcgagaggaa agaagtattt tacaaaatac ttgaaaagcg aaagaaagta
    28141 gtatgctgtc catgcaatac agaaaccgat tctgagaaac tcattgtcaa ctatacagaa
    28201 atacaggtat acaatcagga gaataggtaa aatccataat actatagcta aaaaaatata
    28261 tacggggatc aggcgtctgt atgcccgttc atgtttattc aggatgaata aaatcttctc
    28321 cgcttctgaa tcacacataa cattctcttc ccattttttc tgatacgtaa tgtccacctg
    28381 ttatcaggag tgtttattgc ccagaaatat tctcctgctg tgcctgcgtc ctgagccact
    28441 tttgttctaa tgtcgccagt ctgcgaccgg cactctgtgc ttcagagtct gagacctgcc
    28501 ctttggggtg accatccagg ccataacgtg ggacgccggc gacagctgtc cgctgcagat
    28561 atacgcggcg ggatgtgatg caggacactg cacagagcca ttcgtccatg gtcagttctg
    28621 tctccgggtg ttctgccaga aatgccgaaa tatcattgct tatgccaatt ttcaggggaa
    28681 taaatccacc accttcagca cgaagatgag gaaacagggc ctttattttt tccacgcact
    28741 cgtgagtata ctcacgggtc cagtgggagg gacgtggaaa acggtgtctt ttctttttct
    28801 tcttcctggc tggcgcttcg gggatatgtg tttcgacggc agatgaattt attccggcgg
    28861 gggctgattc tgccgtctgt tcagtaaata caggtgcgat aatatctgtt ttttcggata
    28921 aggacggtgg cgaaaaagta cgacgttttt tcaccacaat gacgggagtt ttcctttctt
    28981 cctgattttt tcccataaat acctcggctg gctggtttta ctctttaata atatcacaat
    29041 ccataaatta gtaaatttcc catcaggtga tttttcatgg ctaataagtc cattctctga
    29101 atatttatac tcgaggtagt tacagttatg gcgtgacagt cgaattgata ttctactgaa
    29161 ccctgacaga aacatggttg atatattctc tcactttaat gttgtcatga tataaataaa
    29221 aacaattcat ttgaaaggat atcagaagaa gatatcgggg cgcacagttc atctctgcct
    29281 ttgtttcatg gggggttctt tgtatatttt acttactgtt taagcatgta cataaggagt
    29341 gctttcatta tgccaatgat acttgtaaag aagaatcccc ggggcaaagt gattcgggag
    29401 cttagcagtg aggaagagac tgcagtaaag acggtctgtg gactgaaaag gccagcaacc
    29461 atggcacaac ataacctggc caatgatctt ctccgggaaa tgcgtgagta tgatgcatgg
    29521 ttacagtgtg actgtattcc gggagattcg ccggccatga attttgctgc tcttaaaaac
    29581 aatacgggta ctctttatct gtccagtttt aatcatgagc atgcacctga atgccccatg
    29641 tatcgtcagt tatccggaaa tgaagaggaa acatccttcg gagcaagccg gcatcccgtt
    29701 agcacccgta taaattacag aaacttttta ccacctgatg acagtaattc tgttatcaga
    29761 ctgcaggccc gctctgctta ccataatggt gagcgctcct ccgttcgcaa gaaacgtccg
    29821 cgccttggcc gtttactcct gtcgcttatt gaagatgcag ggctgaataa actggattcc
    29881 ctggctaatc cccgtatccg gactaattat cgtgagtgtc ttgatgccat acggcaggtt
    29941 acactacagc aggagtatat tcggggaaga gccttatctg aaattatcca tttccggcca
    30001 ggaatgtctg aaaggtctca ggagcggtta atggaaacgc tggagaattc agaacgacac
    30061 tggcctgcca gaagaaagca tatgttcttt cagattttta tggcgcagca catctgtcgt
    30121 gatgctgtgg aaattcactg ggcgaacggt aatattcagg tcatcagacc tgtgcggggg
    30181 atcagtatta atggtgaagc gcagggcgga atacgtcccc cttactgggt tattcttgct
    30241 ttttgccgga gtgccgatgg cagaatcatc tgcagtgaag ggtatgctca tgctctttat
    30301 caactgacat gtccggtgcc tgtggacagc aaactggaac gaaacacgct cactgctctt
    30361 ctgaatgtgg ccagctggct taaaagaaag ccaggtacgc cggaattaag tctggaaagg
    30421 cccctgtttg atacagaagt ttatgttaat ggtgaaaaga aatatgtact gccggatttc
    30481 attgtcacag caagggctcc tgacggaaag acggccagag tggtcatcga aacgatggga
    30541 tatgaagaca gtgattactg cgcgagaaaa tccaggcagc ataccggcat gaagcagatt
    30601 ggtgttctgc ataccgatcc accgaaatgg ctggataacg atcatccccc ttttaagaaa
    30661 catatgtacg gtgtttttat gcatctcagg tactgagata ttttgtggct cagttctgta
    30721 acttttcccg taacattgtc tgttgttacg ggaaagtccg gtttttgtat tgcaccagag
    30781 aatacccaga ctgtgatgct gccacagtgt cagcaggctt tctgaacggt ataacaccac
    30841 ttcatgttaa tgataattac tatcattaaa tcttgacatg ccattttctc cttaataaat
    30901 tagtactgta tatgtatcca tatacgtaag cagttaattc atttgttttc ctcagaggat
    30961 gaaggagata ccgaatgtct gaccctgtac gtattacaaa tcccggtgca gaatcgctgg
    31021 gatatgattc agatggccat gaaattatgg ccgttgatat ttatgtaaac cctccacgtg
    31081 tcgatgtctt tcatggtacc ccgcctgcat ggagttcctt cgggaacaaa accatctggg
    31141 gtggaaacga gtgggtcgat gattccccaa cccgaagtga tatcgaaaaa agggacaagg
    31201 aaatcacagc gtacaaaaac acgctcagcg cgcagcagaa agagaatgag aataagcgta
    31261 ctgaagctgg aaaacgcctt tctgcggcaa ttgctgcaag ggaaaaagat gaaaacacac
    31321 tgaaaacact ccgtgccgga aacgcagatg ccgctgatat tacacgacag gagttcagac
    31381 tcctgcaggc agagctgaga gaatacggat tccgtactga aatcgccgga tatgatgccc
    31441 tccggctgca tacagagagc cggatgctgt ttgctgatgc tgattctctt cgtatatctc
    31501 cccgcgaggc caggtcgtta atcgaacagg ctgaaaaacg gcagaaggat gcgcagaacg
    31561 cagacaagaa ggccgctgat atgcttgctg aatacgagcg cagaaaaggt attctggaca
    31621 cgcggttgtc agagctggaa aaaaatggcg gggcagccct tgccgttctt gatgcacaac
    31681 aggcccgtct gctcgggcag cagacacgga atgacagggc catttcagag gcccggaata
    31741 aactcagttc ggtgacggaa tcgcttaaga cggcccgtaa tgcattaacc agagctgaac
    31801 aacagctgac gcaacagaaa aacacgcctg acggcaaaac gatagtttcc cctgaaaaat
    31861 tcccggggcg ttcatcaaca aatcattcta ttgttgtgag tggtgatccg aggtttgccg
    31921 gtacgataaa aatcacaacc agcgcggtca tcgataaccg tgcaaacctg aattatcttc
    31981 tgacccattc cggtctggac tataaacgca atattctgaa tgaccggaat ccggtggtga
    32041 cagaggatgt ggaaggtgac aagaaaattt ataatgctga agttgctgaa tgggataagt
    32101 tacggcaacg attgcttgat gccagaaata aaatcacctc tgctgaatct gcgataaatt
    32161 cggcgagaaa taacgtcagt gccagaacaa atgaacaaaa gcatgcaaat gacgctctta
    32221 atgccctgtt gaaggaaaaa gagaatatcc gtagccagct tgctgacatc aatcagaaaa
    32281 tagctgaaga gaaaagaaaa agggatgaaa taaatatggt aaaggatgcc ataaaactca
    32341 cctctgattt ctacagaacg atatatgatg agttcggtaa acaagcatcc gaacttgcta
    32401 aggagctggc ttctgtatct caagggaaac agattaagag tgtggatgat gcactgaacg
    32461 cttttgataa attccgtaat aatctgaaca agaaatataa catacaagat cgcatggcca
    32521 tttctaaagc cctggaagct attaatcagg tccatatggc ggagaatttt aagctgttca
    32581 gtaaggcatt tggttttacc ggaaaagtta ttgatcgtta tgatgttgct gtggagttac
    32641 aaaaggctgt aaaaacggac aactggcgtc cattttttgt aaaacttgaa tcactggcag
    32701 caggaagagc tgcttcagca gttacagcat gggcgttttc cgtcatgctg ggaacccctg
    32761 taggtattct gggttttgca attattatgg cggctgtgag tgcgcttgtt aatgataagt
    32821 ttattgagca ggtcaataaa cttattggta tctgacacat aatgatgccc tttcattttc
    32881 ctgaaagggc tttgcagagt ataaaaatta agtaaatggc aaccacagga actgatataa
    32941 cgaaacagaa taattcaaat attgcagtta agcttccgcc aactggattt gtaaagaagt
    33001 ctttttgcca gaattctttc cctgtaaacc ttattaccat tttctgtatt atcctcattg
    33061 aaaaaggaaa agtaatggca ctgataatgc atccgatgaa cgcataaaac attcttgcat
    33121 tttcctggtt atctttccat cccagataaa aaactgtaag aataatgagg attggtatca
    33181 ggcttttcag taaatacttt acagatatat ccagtttcat ctggtttctc ctttattgtt
    33241 atcgtgtacc ggtgcgtgat aacataatca cagcatgata ataataatca ataacaataa
    33301 gctgtgtcac gtttacatca tggaacggag gtgctcctgt gacctttgag cagaaaaaag
    33361 ggcactgttg caaagttagc gatgaggcag ccttttgt
//
LOCUS       DAEEBB010000034        33013 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEEBB010000034 DAEEBB010000000
VERSION     DAEEBB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.034"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="KLF07_001490"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="KLF07_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBD9831544.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="KLF07_001491"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="KLF07_001491"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBD9831545.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="KLF07_001492"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="KLF07_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBD9831546.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="KLF07_001493"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="KLF07_001493"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBD9831547.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="KLF07_001494"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="KLF07_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBD9831548.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="KLF07_001495"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="KLF07_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBD9831549.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="KLF07_001496"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="KLF07_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBD9831550.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="KLF07_001497"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="KLF07_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBD9831551.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="KLF07_001498"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="KLF07_001498"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBD9831552.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="KLF07_001499"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="KLF07_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBD9831553.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="KLF07_001500"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="KLF07_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBD9831554.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="KLF07_001501"
     CDS             complement(13980..14846)
                     /locus_tag="KLF07_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBD9831555.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="KLF07_001502"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="KLF07_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBD9831556.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="KLF07_001503"
     CDS             17484..17819
                     /locus_tag="KLF07_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBD9831557.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="KLF07_001504"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="KLF07_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="KLF07_001505"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="KLF07_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBD9831558.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="KLF07_001506"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="KLF07_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9831559.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="KLF07_001507"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="KLF07_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBD9831560.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="KLF07_001508"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="KLF07_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBD9831561.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="KLF07_001509"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="KLF07_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBD9831562.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="KLF07_001510"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="KLF07_001510"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBD9831563.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="KLF07_001511"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="KLF07_001511"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBD9831564.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="KLF07_001512"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="KLF07_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBD9831565.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="KLF07_001513"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="KLF07_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBD9831566.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="KLF07_001514"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="KLF07_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBD9831567.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="KLF07_001515"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="KLF07_001515"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBD9831568.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="KLF07_001516"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="KLF07_001516"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBD9831569.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..28626
                     /gene="phnO"
                     /locus_tag="KLF07_001517"
     CDS             28192..28626
                     /gene="phnO"
                     /locus_tag="KLF07_001517"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBD9831570.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28628..29386
                     /gene="phnP"
                     /locus_tag="KLF07_001518"
     CDS             28628..29386
                     /gene="phnP"
                     /locus_tag="KLF07_001518"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBD9831571.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29533..29862)
                     /gene="yjdP"
                     /locus_tag="KLF07_001519"
     CDS             complement(29533..29862)
                     /gene="yjdP"
                     /locus_tag="KLF07_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBD9831572.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29931..30248)
                     /locus_tag="KLF07_001520"
     CDS             complement(29931..30248)
                     /locus_tag="KLF07_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBD9831573.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30501..32486
                     /gene="yjcS"
                     /locus_tag="KLF07_001521"
     CDS             30501..32486
                     /gene="yjcS"
                     /locus_tag="KLF07_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBD9831574.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32695..32970
                     /locus_tag="KLF07_001522"
     CDS             32695..32970
                     /locus_tag="KLF07_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831575.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         8025 a         9108 c         8460 g         7420 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg
    28621 ctgtaacatg agtctgaccc tcacgctcac cggcaccggc ggcgcacagg gcgttccggc
    28681 atggggctgc gagtgcgcgg cctgcgaccg agcgcggcgc tcgccgcagt atcgccgcca
    28741 accgtgcagc ggcgtagtga agtttaacga ggcgatcacc ctgatcgacg ccgggctgca
    28801 cgatctcacc gatcgctggt ctcccggatc gttccagcag tttttgctga cgcattatca
    28861 tatggatcac gtccaggggc tgtttccgct gcgctggggc gtgggcgatc cgatcccgat
    28921 ctacggcccg ccggatgaac agggctgcga cgatctgttt aaacatcccg ggctgcttga
    28981 tttcagccac acggtggaac cgtttgtggt gtttgatttg caggggttgc aggtcacgcc
    29041 gctgccgctt aaccactcaa aactcacttt cggctattta ctggaaacgg cgcacagccg
    29101 ggtggcgtgg ctgtctgaca ccgctggctt gccggaaaaa acgctgaaat ttttacgcaa
    29161 taatcagccg caggtaatgg tgatggattg cagtcacccg ccgcgcgcgg atgcaccgcg
    29221 taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct cgccacgggt
    29281 gattctgacc catatcagcc accagtttga tgcgtggctg atggaaaacg cactaccgtc
    29341 agggtttgag gtggggtttg atgggatgga gattggggtg gcgtgatgag agggaatgtg
    29401 cgcgctggcc ccctcaccct aaccctctcc ccagaggggc gaggggaccg attgtgctca
    29461 atattgaata ttgcgctcgt tttctccctc tccccattgg ggtgaggggc gatgcctgct
    29521 ccatacccaa cctcatcgcc catactcatc ttccattctc cgctcttcat cctccagttg
    29581 ccgacgctcc tgatcaagct ggcgctggcg atcgtccagc tgcctgcggc gatcttcaaa
    29641 ctggcggcgg cggtcgtcat attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc
    29701 gtcgtcgcgc gaatcttcat agtctcgccc acggtcaggg ttataagcgt cattaatcgc
    29761 ctgctgaata ttaccaatag tatcgtcgat aatatcagcc tgggccggaa cggtggacag
    29821 cgtgagcagg gtgaataaaa gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga
    29881 aactctatcc acattagtca ttactcatcc atgcaagtag tggatgaatc tcaatttctc
    29941 cgctgctcta ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt
    30001 gcgcccagcc acgcatccac aatcattttt gccagttcga ggccaaccac tcgtgaacca
    30061 aaagccagtg caacttcact ggcataatgc ggataatcag tgcgctctga cgacctggtg
    30121 tctttatcaa tcacctcact cattgaccat gaataaaagg ctggcacagc aacgcagaat
    30181 cctgcggtgc cagcgacatg accgcccatt gctccggtac gttttttaaa aagaaacacc
    30241 ggttgcattg ataaattgtg ctcagtattt gccgaagtac aatatatata ataaatatat
    30301 aaaacacctc atgttattta cagagataat aaattcgcgt aattttccct cttcagcaca
    30361 aatgcctaat aaaatggatc gttcagctca cacttccact tattaaagtc gcaaattccc
    30421 aattatgata accccagctt taaatgacgt attttgcatg gattacatat ttacgacata
    30481 aatgataaat ggagatttac atgaataact ctcggttatt ccgtttgagc agaattgtta
    30541 ttgcgttaac tgccgccagc ggcatgatgg taaataccgc taacgcgaaa gaggaagcga
    30601 aagccgccac tcaatatacc caacaggtta atcagaatta cgccaaatca ttaccgttta
    30661 gcgatcgtca ggattttgac gacgctcagc gtggatttat cgctccgctg ctggatgaag
    30721 gtattctgcg cgatgctaat ggcaaaccat attatcgcgg ggaagattat aaatttgata
    30781 tcaatgcgcc cgcgcccgaa accgttaacc ctagcctgtg gcgtcagtcg cagttaaacg
    30841 gtatttccgg cctgtttaaa gtcaccgaca gaatgtacca ggttcgcggt caggatatct
    30901 ccaacatcac ctttatcgaa ggtgataccg ggattattgt catcgacccg ctggtgacgc
    30961 caccgagcgc aaaagccgcc cttgaccttt acttccaaaa tcgcccgcaa aaaccgattg
    31021 ttgcggttat ttatacccac agccataccg accactatgg cggcgtgaaa ggcattatct
    31081 ctgaagctga tgtgaaatcc ggtaaagtgc aggttatcgc ccctgctggc tttatggacg
    31141 aagccatcag cgaaaacgta ctggcgggta atattatgag ccgccgtgca ctttactcct
    31201 acggcctgct gctggcgcat aaccctcagg gtaacatcgg caatggtctt ggcgtaacgc
    31261 tggcatcggg ctacccgagc atcatcgcac cgaacaaaac catcaccaaa accggtgaga
    31321 agatgattat cgacggcctg gagtttgact tcctgatgac cccaggtagc gaagcaccag
    31381 ccgaaatgca cttctatatt ccggccctga aagcgctgtg taccgccgag aacgccacgc
    31441 ataccctgca caacttctac actctgcgcg gcgcgaaaac ccgcgacacc agcaagtgga
    31501 ccgagtatct gaacgaaacg ctggatatgt ggggtaacga cgcggaagtc ctgtttatgc
    31561 cgcacacctg gccggtctgg ggcaataagc atatcaatga ttatattggt aaatatcgcg
    31621 atactatcaa gtacattcac gaccagaccc tgcacctggc gaaccagggc tacaccatga
    31681 atgaaatcgg cgacatgatt aaactgccgc ctgcacttgc caataactgg gccagccgtg
    31741 gctattacgg ttctgtcagc cacaacgccc gcgcggtgta taacttctat cttggctatt
    31801 acgacggtaa cccggctaac ctgcatccgt atggtcaggt ggagatgggt aaacgttacg
    31861 tgcaggcgct gggcggttct gcccgtgtca tcaacctggc gcaagaagcg aacaagcaag
    31921 gtgattaccg ctggtcggca gaactgctga aacaggtgat tgccgccaac ccgggtgacc
    31981 aggtcgcgaa gaatctgcaa gcgaataact ttgaacagct gggctatcag gccgagtccg
    32041 ccacctggcg cggtttctac ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt
    32101 tcagccacgg caccatcggt tccccggaca ccattcgcgg gatgtcggtc gaaatgctgt
    32161 tcgactttat gtccgttcgc ctcgatagcg cgaaagccgc gggtaaaaat atcagcctga
    32221 acttcaatat gagcaatggc gataacctca acctgacgct gaacgatagc gtgcttaact
    32281 accgtaaaac actgcaatcc caagctgacg cctctttcta catcagccgt gaagatctgc
    32341 acgccgtgct gaccgggcaa gccaaaatgg cggatctggt aaaagcgaag aaagccaaaa
    32401 ttattggcaa tggcgcgaaa ctggaagaaa ttatcgcctg tctggataat ttcgatttgt
    32461 gggtgaatat cgtaacccca aattaatgtc actcgctttt gggggcgctg aagcccccaa
    32521 aagcatttat tttaaaaata tatacagcca attaataaat aaaatatata agttatgaat
    32581 gcggtgactg aataaagcta tttttattca tcgagaaaat agccatactg acgccttcaa
    32641 tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt taatatgcca
    32701 acagttctct ctcgcatggc gatgcaactc aaaaaaacag cctggataat tcccgtcttc
    32761 atggtttcgg gatgctcatt atctccggca atcccggtga tcggtgctta ttatcccagc
    32821 tggtttttct gcgccattgc cagccttatt ttgatgctca tcacgaggcg agttattcag
    32881 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
    32941 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
    33001 ccgaaaaaag ctc
//
LOCUS       DAEEBB010000035        32837 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEEBB010000035 DAEEBB010000000
VERSION     DAEEBB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.035"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            267..1925
                     /locus_tag="KLF07_001523"
     CDS             267..1925
                     /locus_tag="KLF07_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBD9831576.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="KLF07_001524"
     CDS             complement(1922..2878)
                     /locus_tag="KLF07_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBD9831577.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="KLF07_001525"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="KLF07_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBD9831578.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="KLF07_001526"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="KLF07_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBD9831579.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="KLF07_001527"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="KLF07_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBD9831580.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="KLF07_001528"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="KLF07_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBD9831581.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="KLF07_001529"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="KLF07_001529"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBD9831582.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="KLF07_001530"
     tRNA            complement(9740..9827)
                     /locus_tag="KLF07_001530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="KLF07_001531"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="KLF07_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBD9831583.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="KLF07_001532"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="KLF07_001532"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBD9831584.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="KLF07_001533"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="KLF07_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBD9831585.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="KLF07_001534"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="KLF07_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBD9831586.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="KLF07_001535"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="KLF07_001535"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBD9831587.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="KLF07_001536"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="KLF07_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBD9831588.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="KLF07_001537"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="KLF07_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBD9831589.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="KLF07_001538"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="KLF07_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBD9831590.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="KLF07_001539"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="KLF07_001539"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBD9831591.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="KLF07_001540"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="KLF07_001540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBD9831592.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="KLF07_001541"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="KLF07_001541"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBD9831593.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="KLF07_001542"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="KLF07_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD9831594.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="KLF07_001543"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="KLF07_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBD9831595.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /locus_tag="KLF07_001544"
     CDS             complement(28862..29488)
                     /locus_tag="KLF07_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD9831596.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="KLF07_001545"
     CDS             29803..30951
                     /locus_tag="KLF07_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831597.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="KLF07_001546"
                     /pseudo
     CDS             31161..32591
                     /locus_tag="KLF07_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(32592..>32837)
                     /locus_tag="KLF07_001547"
     CDS             complement(32592..>32837)
                     /locus_tag="KLF07_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9831598.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8031 c         8813 g         7936 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccctaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatc
//
LOCUS       DAEEBB010000036        32587 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEEBB010000036 DAEEBB010000000
VERSION     DAEEBB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.036"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            520..759
                     /locus_tag="KLF07_001548"
     CDS             520..759
                     /locus_tag="KLF07_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBD9831599.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="KLF07_001549"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="KLF07_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA"
                     /locus_tag="KLF07_001550"
     CDS             2256..2729
                     /gene="dfrA"
                     /locus_tag="KLF07_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HBD9831600.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="KLF07_001551"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="KLF07_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HBD9831601.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3417..3914
                     /locus_tag="KLF07_001552"
     CDS             3417..3914
                     /locus_tag="KLF07_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831602.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3975..4346
                     /locus_tag="KLF07_001553"
     CDS             3975..4346
                     /locus_tag="KLF07_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831603.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4756..5133
                     /locus_tag="KLF07_001554"
     CDS             4756..5133
                     /locus_tag="KLF07_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831604.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5156..5308
                     /locus_tag="KLF07_001555"
                     /pseudo
     CDS             5156..5308
                     /locus_tag="KLF07_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5364..6980)
                     /locus_tag="KLF07_001556"
     CDS             complement(5364..6980)
                     /locus_tag="KLF07_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HBD9831605.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6981..8507)
                     /locus_tag="KLF07_001557"
     CDS             complement(6981..8507)
                     /locus_tag="KLF07_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HBD9831606.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8510..10177)
                     /locus_tag="KLF07_001558"
     CDS             complement(8510..10177)
                     /locus_tag="KLF07_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD9831607.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10174..12282)
                     /locus_tag="KLF07_001559"
     CDS             complement(10174..12282)
                     /locus_tag="KLF07_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBD9831608.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12269..13090)
                     /locus_tag="KLF07_001560"
     CDS             complement(12269..13090)
                     /locus_tag="KLF07_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HBD9831609.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="KLF07_001561"
     CDS             complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="KLF07_001561"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBD9831610.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="KLF07_001562"
     CDS             complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="KLF07_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBD9831611.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16962..17381)
                     /gene="atpC"
                     /locus_tag="KLF07_001563"
     CDS             complement(16962..17381)
                     /gene="atpC"
                     /locus_tag="KLF07_001563"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBD9831612.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="KLF07_001564"
     CDS             complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="KLF07_001564"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBD9831613.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="KLF07_001565"
     CDS             complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="KLF07_001565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBD9831614.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="KLF07_001566"
     CDS             complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="KLF07_001566"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBD9831615.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="KLF07_001567"
     CDS             complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="KLF07_001567"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBD9831616.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="KLF07_001568"
     CDS             complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="KLF07_001568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBD9831617.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="KLF07_001569"
     CDS             complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="KLF07_001569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBD9831618.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="KLF07_001570"
     CDS             complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="KLF07_001570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBD9831619.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="KLF07_001571"
     CDS             complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="KLF07_001571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBD9831620.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="KLF07_001572"
     CDS             complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="KLF07_001572"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBD9831621.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="KLF07_001573"
     CDS             complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="KLF07_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBD9831622.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="KLF07_001574"
     CDS             complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="KLF07_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBD9831623.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="KLF07_001575"
     CDS             complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="KLF07_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBD9831624.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28564..29556
                     /gene="asnA"
                     /locus_tag="KLF07_001576"
     CDS             28564..29556
                     /gene="asnA"
                     /locus_tag="KLF07_001576"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBD9831625.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="KLF07_001577"
     CDS             complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="KLF07_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBD9831626.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31006..32502)
                     /gene="ravA"
                     /locus_tag="KLF07_001578"
     CDS             complement(31006..32502)
                     /gene="ravA"
                     /locus_tag="KLF07_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBD9831627.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8313 a         8237 c         7556 g         8481 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctagaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttag agagctgggg aagactatgc
     3421 gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat ggcatcgggg caggcacttg
     3481 ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc ctatgactac tccccatcca
     3541 actacgacat ttctccaagc aactacgaca actccataag caattacgac aatagtccat
     3601 caaattacga caactctgag agcaactacg ataatagttc atccaattac gacaatagtc
     3661 gcaacggaaa tcgtaggctt atatatagcg caaatgggtc tcgcactttc gccggctact
     3721 acgtcattgc caacaatggg acaacgaact tcttttccac atctggcaaa aggatgttct
     3781 acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat
     3841 tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga taacggcctg aagatcatgt
     3901 atctaagcaa ctagcctgct ctctaataaa atgttaggcc tcaacatcta gtcgcaagct
     3961 gaggggaacc actagtgtca tacgaacctc caagagacgg ttacacaaac gggtacattg
     4021 ttgatgtcat gtatgacaat cgcccaagta agtatccagc tgtgttcaga acgtacgtcc
     4081 gaattccaga ctatgtcgaa ctagataacg cggaggagct ggccgacgca atcattcgag
     4141 aggttgagtt aaaggaggca tggctcacaa gggtcttggg gaactcagcc gatcctgacg
     4201 cttctgtggg caagtgggac cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa
     4261 gtggggtgca ggcaacatac ttcagtccaa acggtcagct ggtcggctta gtggcataca
     4321 ttgatgccga atggatggat gaatagctct gacgttacga ttcaggtgag acaaccagat
     4381 gggcattgcg tacaagctca gttgccaccg atgtggctac gagtctgatc ttctttacct
     4441 aggccaaggt atggcgatgc ttcctgagca aatagtcgga acgtgcacat gctgcgacaa
     4501 tctaacgacg atttctataa atgaaagcta cggagtatgc tcactttgtg gggctaagga
     4561 tcgtgtgata tttacaagct atcagcatga ggcaaggcca agaaatccct gctatctaga
     4621 gagaagtttc aaatatcaat gcccaagatg ccatgccttc agtatggagc ctccggatct
     4681 accggagatg ctttgggact aatgctccgc tttgcctcgg gtatccgttc gacctatgtt
     4741 taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct atgcactttg tgacaagttg
     4801 gaaagcaaaa aagttgatga agtcgtacga aatcttgttt ctagcggctg gaatattagg
     4861 gaagtggcct gggaagcttc gtcctttgat catgaaatgc cctactttga cacggggcgg
     4921 gacttcgata ttggatttcc tgacacgctg tcatccaaga ttgagttttt gaccgcgaat
     4981 ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc aggctctaat ttttgccaag
     5041 gcgtccaaac atgtcgccgc gctcaagaac tcattggatt actactatgg atcgtctgta
     5101 atcccccctc aaaaccacgg aggctatttg tagaaaattc cattaatatg aactcaaggt
     5161 gttccctacg gacgaatcag cgatgaaggt ggtgtacctg gctatccagg cggcgaccaa
     5221 gaaatggacg atgccgatcc gcaactggaa gccggccatg aaccgtttta tgatcgaatt
     5281 tggtgaccgt ttaaatggcc acctttaact gaccggcaaa agcacttaca caaaagggtt
     5341 tacaggctct ttcagtcatt cctttaatgc gtaaattgct ctctaaccac tctctcagcc
     5401 cacccttcca tatcctctct cgatacttgc agctccccca acccctttgg atgattcacc
     5461 cctctatgcc cgtcttgtcc atataattga tcaaacaaac tatttggcca attcaatgag
     5521 ctcttcacca ctccacgccg tatcttttca aaatcattcc gccatgtttc gctatcaacg
     5581 ccactcaaca ctttggtaga aagcattttc accccatccg atgcatccac ctctagcaat
     5641 acaaattcac tgccattacg ttgcacccca acggctttaa tcactcttgg tgagccatct
     5701 ttacacagat gtaatcggct acgcccaact tttggcaaaa ccaaggtttc ttcaaaaagc
     5761 acccgacatg caaatttagt ttttagaatt gaaagtagct catcaaaagc agcaaatcga
     5821 ttagcaaaaa tagagttgta attggttgca tcctgcttcc cacccacatc tgccgctgct
     5881 aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat tactattttc acttgttgtt
     5941 gcttcttctt tttctaaaat agtttggcta ctttttcttg ccctgcgaga tttttcaatt
     6001 cgactaggtc tactaaaact tatccatgtc ggctcagcct ctatgactcg tgtgtctgtg
     6061 tctatattgg ctgtttcctc atcgtccaac tgtagatgct catcatcaac aactgactct
     6121 gttgaaaccg ctatgtgctg cgtactacca tcacctgcct ccttttcctg aaaagaggca
     6181 tggctaatag ctgttgtgct aggaagcata gcgttgattt ctaaacctac tatttcctca
     6241 actaaataat ccttatcctc gttagaagaa cgtcctttta catgtaattt ccaaccctgc
     6301 atgggcggag ggtcaaagct aaagcaccag ctttcaacgc cgttcttaat ttctctattt
     6361 tgttgatagt gcctaaaaat actttcatac gaatccataa catcttgatc cgaaaacaac
     6421 caaaccaaaa gctgcactac ggccgactgc tctaacgccc ctttaggaaa cgatgaactg
     6481 ggtaagatcc ttatctctaa atgatctcgc tcaacctcat actgaacgtc gaactcttgc
     6541 tgtaaagcgg tactgctcaa acagcttcga cagaaatagg agttaattaa aaataatgac
     6601 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     6661 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     6721 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     6781 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     6841 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     6901 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     6961 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     7021 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     7081 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     7141 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     7201 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     7261 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     7321 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     7381 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     7441 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     7501 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     7561 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     7621 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     7681 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     7741 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     7801 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     7861 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     7921 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     7981 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     8041 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     8101 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     8161 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     8221 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     8281 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     8341 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     8401 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     8461 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     8521 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     8581 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     8641 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     8701 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     8761 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     8821 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     8881 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     8941 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     9001 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     9061 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     9121 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     9181 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     9241 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     9301 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     9361 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     9421 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     9481 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     9541 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     9601 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     9661 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     9721 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     9781 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     9841 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     9901 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     9961 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
    10021 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
    10081 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
    10141 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
    10201 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
    10261 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
    10321 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
    10381 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
    10441 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
    10501 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
    10561 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
    10621 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
    10681 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
    10741 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
    10801 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
    10861 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
    10921 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
    10981 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
    11041 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
    11101 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
    11161 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
    11221 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    11281 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    11341 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    11401 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    11461 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    11521 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    11581 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    11641 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    11701 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    11761 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    11821 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    11881 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    11941 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    12001 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    12061 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    12121 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    12181 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    12241 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    12301 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    12361 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    12421 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    12481 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    12541 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    12601 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    12661 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    12721 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    12781 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    12841 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    12901 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    12961 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    13021 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    13081 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    13141 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    13201 ttaagacttt attgtccgcc cacaagccct tttttatcta tgatagtgat tactcaaccg
    13261 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
    13321 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
    13381 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
    13441 agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
    13501 gcaattcgtt gttcggtgca acgacgataa ccggcatatc ggcatcaata agcgccagcg
    13561 gaccgtgttt cagttcacca gcagcgtagg cttcagcgtg aatgtaggag atctctttca
    13621 gcttcaatgc gccttccagt gcgattgggt actgatcgcc acggcccagg aacagtgcgt
    13681 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gatagcatct
    13741 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
    13801 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
    13861 tgaatgcttt agtggacgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
    13921 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
    13981 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
    14041 aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat tcagaggcga
    14101 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
    14161 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
    14221 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
    14281 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
    14341 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
    14401 cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
    14461 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
    14521 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
    14581 ggtgacggga gtccatgatc actgtaccgt acgcaccgcg cagctgcggg atagcacgca
    14641 gaacagcctc acgcagagtc ccgccttgtt tcagctccca gttcaccaga tgggcaatca
    14701 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
    14761 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
    14821 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc
    14881 cgccatccag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
    14941 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
    15001 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
    15061 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
    15121 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    15181 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
    15241 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
    15301 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    15361 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
    15421 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    15481 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgcccga ctttcgagcc
    15541 tttacccaga cgcgcttttt tcatctcaac gaagttacca acgtgtgcac cttccagcaa
    15601 ctcagcacca ggacgcaaac gagcaaacgg gccaatagta caggccgctg ccagattcgc
    15661 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    15721 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    15781 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    15841 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
    15901 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
    15961 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
    16021 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    16081 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    16141 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
    16201 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    16261 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    16321 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
    16381 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
    16441 gtgaccgtac accaagtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    16501 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    16561 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    16621 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    16681 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
    16741 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    16801 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    16861 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
    16921 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
    16981 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
    17041 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
    17101 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
    17161 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
    17221 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
    17281 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
    17341 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
    17401 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
    17461 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
    17521 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
    17581 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
    17641 catccatacc caagatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
    17701 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
    17761 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
    17821 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
    17881 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
    17941 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
    18001 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
    18061 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
    18121 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
    18181 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
    18241 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
    18301 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
    18361 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
    18421 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
    18481 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
    18541 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
    18601 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
    18661 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
    18721 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
    18781 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
    18841 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
    18901 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
    18961 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
    19021 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
    19081 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
    19141 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaagc
    19201 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
    19261 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
    19321 gatcattgcg aggtcgcatt gaacgctttt gtcggtccag gtcttcattt ccgccagtag
    19381 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
    19441 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
    19501 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
    19561 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
    19621 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
    19681 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
    19741 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
    19801 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
    19861 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
    19921 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
    19981 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
    20041 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
    20101 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
    20161 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
    20221 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
    20281 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
    20341 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
    20401 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
    20461 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
    20521 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
    20581 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
    20641 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
    20701 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
    20761 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
    20821 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
    20881 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
    20941 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
    21001 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
    21061 tcgcgctcaa ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
    21121 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
    21181 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
    21241 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
    21301 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
    21361 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
    21421 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
    21481 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
    21541 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
    21601 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
    21661 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
    21721 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
    21781 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
    21841 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
    21901 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
    21961 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
    22021 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
    22081 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
    22141 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
    22201 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
    22261 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
    22321 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
    22381 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
    22441 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
    22501 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
    22561 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
    22621 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
    22681 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
    22741 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
    22801 cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag
    22861 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
    22921 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
    22981 caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac
    23041 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
    23101 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
    23161 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    23221 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    23281 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    23341 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    23401 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    23461 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    23521 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    23581 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    23641 cggcctttcg ctggtgtata cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    23701 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    23761 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    23821 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    23881 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    23941 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    24001 aaatattttt ccagtgcata caattgcgac ttttccgcta accctgttcg atcatgaaaa
    24061 actgtaaata acgcgtaaac actagtgata aagcgtgctt cagatcacat attgcgcatg
    24121 ttcgcgcaca gtatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    24181 agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga
    24241 catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg
    24301 atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgata
    24361 caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac ttggttgttg
    24421 ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc
    24481 tttaatcacc accagatgac gttcgccatc cagagctgga acctgaagtt taaccactga
    24541 ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag
    24601 cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag
    24661 agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac
    24721 cctgctctgt actggctcaa tattctccag tttaagctca tgttgcacct gacgaaggaa
    24781 acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga
    24841 gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt caccttgcag
    24901 atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg
    24961 gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag
    25021 ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt
    25081 gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg cttaccaggc
    25141 atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag
    25201 aatggagata gccgcaggcg tgacgccaga aatacgcgaa gcctggccga tagaggccgg
    25261 tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc
    25321 cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct catcttgctg
    25381 gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc
    25441 tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg
    25501 acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac
    25561 ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg
    25621 ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag
    25681 ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgcagca tcagacgata
    25741 ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc
    25801 aaccagtacg ccgagatacg cctgagaacg cgccggagcc caaccttctt tgtcagcaga
    25861 cagacgggca gcgttaagac cgaccagcaa accttgcgca gcggcttctt cgtaaccggt
    25921 agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg
    25981 tttcaggtcg cgtggatcga agaagtcata ctcaatggca taacccggac gcacgatctt
    26041 cgcgttttcc atcccctgca tggagcggac gatttgcatc tgcacatcga acggcaggct
    26101 ggtggagata ccgttcggat aaatttcgtt agaggtcagc ccttccggtt caaggaagat
    26161 ctgatgctga tttctgtcgg cgaagcgcat gactttgtct tcgatcgatg ggcagtagcg
    26221 tgggccgaca ccttcgatca cccctgcgta cattgggcta cgatcgaggt tactgcggat
    26281 cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct ggggatgctg
    26341 ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct gttgcgccag
    26401 cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc
    26461 aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc
    26521 acgaccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga acgtcccaac
    26581 ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag caccgaccac
    26641 gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg
    26701 gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt
    26761 agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac ccgcttgatc
    26821 gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc
    26881 aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa
    26941 aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc ctgcatgacc
    27001 cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa
    27061 cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga
    27121 gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat
    27181 tgtgatctct tattaggatc gcactaccct gtggataaca aggatccggc ttttaagatc
    27241 aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg
    27301 gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc cttggataac
    27361 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
    27421 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
    27481 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
    27541 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac taccaatacc
    27601 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
    27661 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
    27721 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
    27781 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
    27841 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
    27901 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgattatggc
    27961 ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
    28021 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatt
    28081 acttttataa agatgctgta gtggcctgtt gtgtaatagg cttcagtgac ttcagcaagg
    28141 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
    28201 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
    28261 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
    28321 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
    28381 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
    28441 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
    28501 ttattgcatg tttgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
    28561 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
    28621 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
    28681 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
    28741 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
    28801 accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt
    28861 cgccccgatg aagaccgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
    28921 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
    28981 atctgggcgg gaattaaagc aaccgaagct gcggttagcg aagagtttgg cctggcaccg
    29041 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
    29101 ctcgatgcca aagggcgtga gcgggcgata gcgaaagatc ttggcgcggt attccttgtc
    29161 gggattggcg gcaagctgag cgatggtcat cgccacgacg tgcgcgcacc ggattatgat
    29221 gactggagca ccccgtcaga gctgggtcat gcgggtctga acggcgatat tctggtgtgg
    29281 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
    29341 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat
    29401 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
    29461 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg tgtatggcca
    29521 gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
    29581 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
    29641 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
    29701 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
    29761 ccaccgcatc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
    29821 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
    29881 tgccttgggg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
    29941 agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
    30001 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
    30061 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
    30121 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
    30181 ccagttccgt tatccctagt gtcgccagtt ctggcggcag aagacgtaaa atatcatcgc
    30241 tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc atggtgcgga
    30301 aggtttccat ctgcgcatcg ttgcgcggta ttgatttggc ttcccgagaa cgccccagct
    30361 gctctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
    30421 actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcag
    30481 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcttgaactt
    30541 cactcaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
    30601 cgatcagact taaacgccaa cgctggagaa aaagcgtgtg taacgctgaa gttattgtgc
    30661 tgttagcatc aaccaactgc cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta
    30721 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
    30781 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
    30841 gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagg cgtgggaatt
    30901 tttcaaagaa gaccgccagc tgcggtgagg ccagcagtgc gatgatcatc tcttcgatca
    30961 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
    31021 ctggtgaatc tgtgcaccga catcctgtag gctggcttca atgcgaccta accagtcgcc
    31081 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcgct
    31141 ttcaagctct tccagttgtt gttttatttc accaggcaga ccttcagccg atgaaccggg
    31201 aagtgccagc gtactgcctt gtaaacttac atcgcgaacg acaagatgtt gggcgctatc
    31261 aacttccaga ttcagtttct gggcaaagcc gataccgttc agtttaccgc gaatttcacc
    31321 acctttgctc agccactgtt ccagcgcgct acgctcaaag gagatatgaa ccacttccat
    31381 atcatgcagt tttaacggtt tttgcagcag tagagtcaga gtggaagcag taacgttaac
    31441 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
    31501 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
    31561 agtcaacatc ccttgctgtt gccaggcatg accggtcatc aatacatcaa tttgttgttg
    31621 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
    31681 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc ggatcgcttt
    31741 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gcaggcgcag
    31801 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
    31861 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
    31921 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
    31981 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt
    32041 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
    32101 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
    32161 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
    32221 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
    32281 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
    32341 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
    32401 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
    32461 caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt
    32521 cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc
    32581 tcaaagg
//
LOCUS       DAEEBB010000037        32532 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEEBB010000037 DAEEBB010000000
VERSION     DAEEBB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.037"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..94
                     /locus_tag="KLF07_001579"
     CDS             <1..94
                     /locus_tag="KLF07_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9831628.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="KLF07_001580"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="KLF07_001580"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD9831629.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="KLF07_001581"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="KLF07_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBD9831630.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="KLF07_001582"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="KLF07_001582"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBD9831631.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="KLF07_001583"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="KLF07_001583"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBD9831632.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="KLF07_001584"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="KLF07_001584"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBD9831633.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="KLF07_001585"
     CDS             7105..8007
                     /locus_tag="KLF07_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBD9831634.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="KLF07_001586"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="KLF07_001586"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBD9831635.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="KLF07_001587"
     CDS             complement(8869..10158)
                     /locus_tag="KLF07_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831636.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="KLF07_001588"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="KLF07_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="KLF07_001589"
     CDS             complement(11434..12216)
                     /locus_tag="KLF07_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBD9831637.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="KLF07_001590"
     CDS             complement(12436..13638)
                     /locus_tag="KLF07_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBD9831638.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="KLF07_001591"
     CDS             complement(13738..14280)
                     /locus_tag="KLF07_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831639.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="KLF07_001592"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="KLF07_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBD9831640.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /locus_tag="KLF07_001593"
     CDS             complement(15023..15625)
                     /locus_tag="KLF07_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD9831641.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="KLF07_001594"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="KLF07_001594"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBD9831642.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="KLF07_001595"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="KLF07_001595"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBD9831643.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="KLF07_001596"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="KLF07_001596"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBD9831644.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /locus_tag="KLF07_001597"
     CDS             20023..20913
                     /locus_tag="KLF07_001597"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBD9831645.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="KLF07_001598"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="KLF07_001598"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBD9831646.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="KLF07_001599"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="KLF07_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBD9831647.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="KLF07_001600"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="KLF07_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBD9831648.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="KLF07_001601"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="KLF07_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBD9831649.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="KLF07_001602"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="KLF07_001602"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBD9831650.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="KLF07_001603"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="KLF07_001603"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBD9831651.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="KLF07_001604"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="KLF07_001604"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBD9831652.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="KLF07_001605"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="KLF07_001605"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBD9831653.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="KLF07_001606"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="KLF07_001606"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBD9831654.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="KLF07_001607"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="KLF07_001607"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBD9831655.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="KLF07_001608"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="KLF07_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBD9831656.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31548)
                     /locus_tag="KLF07_001609"
                     /pseudo
     CDS             complement(31087..31548)
                     /locus_tag="KLF07_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31613..32530
                     /gene="rbn"
                     /locus_tag="KLF07_001610"
     CDS             31613..32530
                     /gene="rbn"
                     /locus_tag="KLF07_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBD9831657.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8805 c         8139 g         7692 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggactggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccgcc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccagct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca ggatgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    32221 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    32281 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    32341 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    32401 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    32461 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    32521 taacgtttaa ct
//
LOCUS       DAEEBB010000038        31792 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEEBB010000038 DAEEBB010000000
VERSION     DAEEBB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.038"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="KLF07_001611"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="KLF07_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBD9831658.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="KLF07_001612"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="KLF07_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBD9831659.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="KLF07_001613"
     CDS             1894..2751
                     /locus_tag="KLF07_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9831660.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="KLF07_001614"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="KLF07_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="KLF07_001615"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="KLF07_001615"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD9831661.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="KLF07_001616"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="KLF07_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="KLF07_001617"
     CDS             complement(7411..8658)
                     /locus_tag="KLF07_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBD9831662.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="KLF07_001618"
     CDS             complement(8680..9006)
                     /locus_tag="KLF07_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBD9831663.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="KLF07_001619"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="KLF07_001619"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBD9831664.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="KLF07_001620"
     CDS             10395..11651
                     /locus_tag="KLF07_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBD9831665.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="KLF07_001621"
     CDS             11766..12092
                     /locus_tag="KLF07_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD9831666.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="KLF07_001622"
     CDS             complement(12233..13471)
                     /locus_tag="KLF07_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBD9831667.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="KLF07_001623"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="KLF07_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBD9831668.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="KLF07_001624"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="KLF07_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="KLF07_001625"
     tRNA            complement(17456..17531)
                     /locus_tag="KLF07_001625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="KLF07_001626"
     tRNA            complement(17571..17646)
                     /locus_tag="KLF07_001626"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="KLF07_001627"
     CDS             17867..18226
                     /locus_tag="KLF07_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD9831669.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="KLF07_001628"
     CDS             18228..18620
                     /locus_tag="KLF07_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD9831670.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="KLF07_001629"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="KLF07_001629"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBD9831671.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="KLF07_001630"
     tRNA            20346..20421
                     /locus_tag="KLF07_001630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20466..20541
                     /locus_tag="KLF07_001631"
     tRNA            20466..20541
                     /locus_tag="KLF07_001631"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20499..20501,aa:Val,seq:tac)
     gene            20588..20663
                     /locus_tag="KLF07_001632"
     tRNA            20588..20663
                     /locus_tag="KLF07_001632"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20621..20623,aa:Val,seq:tac)
     gene            20668..20743
                     /locus_tag="KLF07_001633"
     tRNA            20668..20743
                     /locus_tag="KLF07_001633"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20701..20703,aa:Lys,seq:ttt)
     gene            20774..20899
                     /locus_tag="KLF07_001634"
     ncRNA           20774..20899
                     /ncRNA_class="other"
                     /locus_tag="KLF07_001634"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21019..21360
                     /locus_tag="KLF07_001635"
     CDS             21019..21360
                     /locus_tag="KLF07_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBD9831672.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21351..22277)
                     /locus_tag="KLF07_001636"
     CDS             complement(21351..22277)
                     /locus_tag="KLF07_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9831673.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22367..23365
                     /locus_tag="KLF07_001637"
     CDS             22367..23365
                     /locus_tag="KLF07_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBD9831674.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23362..23580)
                     /locus_tag="KLF07_001638"
     CDS             complement(23362..23580)
                     /locus_tag="KLF07_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBD9831675.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23582..25597)
                     /gene="ligA"
                     /locus_tag="KLF07_001639"
     CDS             complement(23582..25597)
                     /gene="ligA"
                     /locus_tag="KLF07_001639"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBD9831676.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25668..26654)
                     /gene="zipA"
                     /locus_tag="KLF07_001640"
     CDS             complement(25668..26654)
                     /gene="zipA"
                     /locus_tag="KLF07_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBD9831677.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26884..27645
                     /gene="cysZ"
                     /locus_tag="KLF07_001641"
     CDS             26884..27645
                     /gene="cysZ"
                     /locus_tag="KLF07_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBD9831678.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27830..28801
                     /gene="cysK"
                     /locus_tag="KLF07_001642"
     CDS             27830..28801
                     /gene="cysK"
                     /locus_tag="KLF07_001642"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBD9831679.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29185..29442
                     /gene="ptsH"
                     /locus_tag="KLF07_001643"
     CDS             29185..29442
                     /gene="ptsH"
                     /locus_tag="KLF07_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBD9831680.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29487..31214
                     /gene="ptsI"
                     /locus_tag="KLF07_001644"
     CDS             29487..31214
                     /gene="ptsI"
                     /locus_tag="KLF07_001644"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBD9831681.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31255..31764
                     /gene="crr"
                     /locus_tag="KLF07_001645"
     CDS             31255..31764
                     /gene="crr"
                     /locus_tag="KLF07_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBD9831682.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7842 a         8383 c         7906 g         7661 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aactacttta tgtagtctcc gccgtgtagc aagaaattga
    20461 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20521 cgatcccgtc atcacccacc aatttctcgc cagctaaatt tcttgtaaaa atgtgaagta
    20581 ccgaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    20641 ttcgatcccg tcatcaccca ccacttcggg tcgttagctc agttggtaga gcagttgact
    20701 tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct
    20761 aaaggcgctt ttttgctatc tgcgatactc aaagattcga acctgcagca ggtttgagtt
    20821 gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc gaagggcgag cgaagcgagt
    20881 catcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
    20941 atttgtaaga ttgatttttc ttctcaagtt ccttgttctt ctgccgatat tacctcgtac
    21001 agtgacgagg actgaacaat ggccactatc tcttcaactt caattccttc catacaaaca
    21061 cagagtagta acagggcatc acaaggtagc gatgttgctt caaaaatcgc gcggataagt
    21121 cagcaaatca tcaaactcac tcaacaaatt aaagagattg tagacactag cggcagtgct
    21181 gaagacaaac aaaaacaggc cgaattgatt caacagcaaa tcaccctact ggaaacacaa
    21241 ctggcgcagt tgcagagaca gcaggcagaa aaggcgcagg agaaagagca gcgtttgtct
    21301 ctgaatgtat cccttctaaa tcctgttgaa aatacgactc atatcgatat ctatatctga
    21361 tacaacggag ccccttcccg accagccatt agcgcattgc cctggtctcg taccacgtcc
    21421 cataacgctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg
    21481 cgttcaacaa ccggcgtaat gcgctttacc accagtgggc taccttcagg caatggtagc
    21541 gccagtgcgg gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc
    21601 ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca
    21661 atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc
    21721 tgccagggta cataatcttc cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa
    21781 ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg
    21841 caaaaatcga catctccctg gcgaatactt tccatcaccc actgctgcgg acgatcgtgc
    21901 aggacaaact gaatatctgg atagcggcga tggctttcag caatgcattg cggaataaga
    21961 tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata
    22021 cgcccggtat cgcgcaacgt gctgttaagt tcatccagca gtcgctcaag acgcaaagcc
    22081 aactgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caggcgaaca
    22141 ccagtatgat tttccagctc cttcacactg tgacttactg ccgactggct caagccgata
    22201 cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt
    22261 tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct
    22321 caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg
    22381 tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc
    22441 ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcta ttgccctgct
    22501 gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg
    22561 gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc tgggtgtact
    22621 gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt
    22681 gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa
    22741 cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg ctggggattt tcctttcacc
    22801 attgctagtt ggtctggtga tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg
    22861 taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat
    22921 tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct
    22981 gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg
    23041 ctggggatca ttgctgttta tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt
    23101 agttaacgtc tttatggcac gccgactggg cttcaataag gcagatgaaa ttactatcgt
    23161 cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc
    23221 cacatcggtg atcggtatga tggtgctgcc cctgatgatt ttccatcaga tccaattgat
    23281 ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcgcagca
    23341 ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag
    23401 cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa
    23461 ctcatctttg cgggcaaacc acagcagata ctcctccggc aggtcgatta agcgacgccc
    23521 tttgtatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat
    23581 ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct
    23641 gcgccttcgc cagtttagat cctgcagctt cacccgctat caccagatcg gttttcttcg
    23701 acacgctgcc cgcgactttc gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg
    23761 acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct
    23821 cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga
    23881 tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc
    23941 caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcact tccagcgtgc
    24001 cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat
    24061 agaggaagcg agcaaaggtg gtttctttcg ctttttccag cgcgttaacc acgttttgtg
    24121 ccgattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat
    24181 ctgccggagt gtggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc
    24241 cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgcac
    24301 cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag
    24361 aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag
    24421 aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat
    24481 cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca
    24541 ccagcacgcc agcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac
    24601 gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa
    24661 acgctaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgttccagtg
    24721 agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca
    24781 ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga
    24841 ccggcaaccc ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc
    24901 caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac
    24961 gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc
    25021 cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt
    25081 caccacgcac ttccagacgc gccgggatat tctctccgtg cagcttcagc ggaatggcgc
    25141 gaatagtacg cacattagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg
    25201 cactgactaa aacgccattt tcatacagaa tactgacggc aaggccatcc agcttcagct
    25261 cacagcacca ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag
    25321 caagaaagct ttcttcatca aaaacgttat ccagtgacag cattggtact tcatggcgta
    25381 tctggctgaa agccgccagc ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa
    25441 tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag
    25501 cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg cgaagcgtcg
    25561 ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac
    25621 ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg
    25681 tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg
    25741 cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc
    25801 atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa gatagtgaca
    25861 cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc
    25921 aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata
    25981 tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg
    26041 ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc
    26101 ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc
    26161 tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc
    26221 tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc
    26281 atcggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
    26341 atcggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga
    26401 cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc
    26461 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
    26521 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
    26581 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
    26641 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
    26701 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
    26761 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
    26821 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
    26881 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
    26941 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
    27001 attttgttga tggggggcgc attctggtgg ctctttacac agctcgatgt ctggatcccg
    27061 actctcatga gttacgttcc ggactggcta caatggctga gttatctgtt gtggcctctg
    27121 gctgtcatct ctgtgctgtt agtgtttggc tatttcttct ccacgattgc taactggatt
    27181 gccgctccgt ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca
    27241 ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
    27301 tggcaaaaat ttgcctggta tctgccgcgc gcaattgtat tactaattct ttacttcatc
    27361 cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
    27421 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
    27481 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt
    27541 acgatgatcc cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg
    27601 atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat
    27661 tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
    27721 gtcattattt cccttctgta tatagatatg ctaaatcctt acttccgcat attctctgag
    27781 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat
    27841 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg
    27901 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
    27961 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
    28021 actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
    28081 cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
    28141 gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat
    28201 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
    28261 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga tctgggaaga
    28321 taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg
    28381 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga
    28441 gccaaccgat tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggccc
    28501 gcataaaatt cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt
    28561 cgataaagtc attggcatca ccaatgaaga agccatttct accgcccgtc gtctaatgga
    28621 agaagaaggt attcttgcag gtatctcttc tggagcagct gttgccgcgg cgttgaaact
    28681 acaagaagat gaaagcttta ccaataagaa tattgtggtt attctaccat catcgggtga
    28741 gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta
    28801 atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
    28861 ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
    28921 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
    28981 tgatttggtt caattcttcc tttaacggca taatgtttaa tgacgtacga aacgtcagcg
    29041 gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac
    29101 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
    29161 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
    29221 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
    29281 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
    29341 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
    29401 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
    29461 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
    29521 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
    29581 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
    29641 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
    29701 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
    29761 tgattaaaga taaacacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
    29821 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
    29881 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca
    29941 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
    30001 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
    30061 tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg
    30121 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
    30181 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
    30241 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtat
    30301 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
    30361 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag
    30421 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc
    30481 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
    30541 acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg
    30601 atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga
    30661 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
    30721 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
    30781 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
    30841 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    30901 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    30961 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    31021 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    31081 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    31141 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    31201 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    31261 ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag
    31321 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    31381 gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg
    31441 cctgtagacg gtaccattgg taaaatcttt gaaaccaatc acgcgttctc tatcgaatct
    31501 gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    31561 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa
    31621 tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    31681 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc
    31741 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc ca
//
LOCUS       DAEEBB010000039        30255 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEEBB010000039 DAEEBB010000000
VERSION     DAEEBB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.039"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..221
                     /gene="tuf"
                     /locus_tag="KLF07_001646"
     CDS             <1..221
                     /gene="tuf"
                     /locus_tag="KLF07_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD9831683.1"
                     /translation="FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            451..834
                     /gene="secE"
                     /locus_tag="KLF07_001647"
     CDS             451..834
                     /gene="secE"
                     /locus_tag="KLF07_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBD9831684.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            836..1381
                     /gene="nusG"
                     /locus_tag="KLF07_001648"
     CDS             836..1381
                     /gene="nusG"
                     /locus_tag="KLF07_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBD9831685.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1540..1968
                     /gene="rplK"
                     /locus_tag="KLF07_001649"
     CDS             1540..1968
                     /gene="rplK"
                     /locus_tag="KLF07_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBD9831686.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1972..2676
                     /gene="rplA"
                     /locus_tag="KLF07_001650"
     CDS             1972..2676
                     /gene="rplA"
                     /locus_tag="KLF07_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBD9831687.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3089..3586
                     /gene="rplJ"
                     /locus_tag="KLF07_001651"
     CDS             3089..3586
                     /gene="rplJ"
                     /locus_tag="KLF07_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBD9831688.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3653..4018
                     /gene="rplL"
                     /locus_tag="KLF07_001652"
     CDS             3653..4018
                     /gene="rplL"
                     /locus_tag="KLF07_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBD9831689.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4338..8366
                     /gene="rpoB"
                     /locus_tag="KLF07_001653"
     CDS             4338..8366
                     /gene="rpoB"
                     /locus_tag="KLF07_001653"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBD9831690.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8443..12666
                     /gene="rpoC"
                     /locus_tag="KLF07_001654"
     CDS             8443..12666
                     /gene="rpoC"
                     /locus_tag="KLF07_001654"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBD9831691.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12908..14041)
                     /gene="thiH"
                     /locus_tag="KLF07_001655"
     CDS             complement(12908..14041)
                     /gene="thiH"
                     /locus_tag="KLF07_001655"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBD9831692.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14038..14808)
                     /gene="thiG"
                     /locus_tag="KLF07_001656"
     CDS             complement(14038..14808)
                     /gene="thiG"
                     /locus_tag="KLF07_001656"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBD9831693.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14810..15010)
                     /gene="thiS"
                     /locus_tag="KLF07_001657"
     CDS             complement(14810..15010)
                     /gene="thiS"
                     /locus_tag="KLF07_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBD9831694.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14994..15749)
                     /gene="thiF"
                     /locus_tag="KLF07_001658"
     CDS             complement(14994..15749)
                     /gene="thiF"
                     /locus_tag="KLF07_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBD9831695.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15742..16377)
                     /gene="thiE"
                     /locus_tag="KLF07_001659"
     CDS             complement(15742..16377)
                     /gene="thiE"
                     /locus_tag="KLF07_001659"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBD9831696.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16377..18272)
                     /gene="thiC"
                     /locus_tag="KLF07_001660"
     CDS             complement(16377..18272)
                     /gene="thiC"
                     /locus_tag="KLF07_001660"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBD9831697.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18353..18453)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18506..18982)
                     /gene="rsd"
                     /locus_tag="KLF07_001661"
     CDS             complement(18506..18982)
                     /gene="rsd"
                     /locus_tag="KLF07_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBD9831698.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19077..19850
                     /gene="nudC"
                     /locus_tag="KLF07_001662"
     CDS             19077..19850
                     /gene="nudC"
                     /locus_tag="KLF07_001662"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBD9831699.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19890..20954
                     /gene="hemE"
                     /locus_tag="KLF07_001663"
     CDS             19890..20954
                     /gene="hemE"
                     /locus_tag="KLF07_001663"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBD9831700.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20964..21635
                     /gene="nfi"
                     /locus_tag="KLF07_001664"
     CDS             20964..21635
                     /gene="nfi"
                     /locus_tag="KLF07_001664"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBD9831701.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21678..22268
                     /locus_tag="KLF07_001665"
     CDS             21678..22268
                     /locus_tag="KLF07_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBD9831702.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22455..22727
                     /gene="hupA"
                     /locus_tag="KLF07_001666"
     CDS             22455..22727
                     /gene="hupA"
                     /locus_tag="KLF07_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBD9831703.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22740..23435
                     /locus_tag="KLF07_001667"
     CDS             22740..23435
                     /locus_tag="KLF07_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBD9831704.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23437..23862)
                     /gene="zraP"
                     /locus_tag="KLF07_001668"
     CDS             complement(23437..23862)
                     /gene="zraP"
                     /locus_tag="KLF07_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBD9831705.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24100..25476
                     /gene="zraS"
                     /locus_tag="KLF07_001669"
     CDS             24100..25476
                     /gene="zraS"
                     /locus_tag="KLF07_001669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBD9831706.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25473..26798
                     /gene="zraR"
                     /locus_tag="KLF07_001670"
     CDS             25473..26798
                     /gene="zraR"
                     /locus_tag="KLF07_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBD9831707.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26795..28084)
                     /gene="purD"
                     /locus_tag="KLF07_001671"
     CDS             complement(26795..28084)
                     /gene="purD"
                     /locus_tag="KLF07_001671"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBD9831708.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28096..29685)
                     /gene="purH"
                     /locus_tag="KLF07_001672"
     CDS             complement(28096..29685)
                     /gene="purH"
                     /locus_tag="KLF07_001672"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBD9831709.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7093 a         7885 c         8329 g         6948 t
ORIGIN      
        1 cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg actggtacca
       61 tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa atggttgtta
      121 ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc
      181 gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata acatttgacg
      241 caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt accagaacct
      301 ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt tgagggcgta
      361 taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat
      421 gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc tcaaggaagc
      481 gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct ggtggcgatt
      541 gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt
      601 ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc taccgttgct
      661 tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca
      721 ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat cctgtgggga
      781 ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt ctgagatgtc
      841 tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt
      901 agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt ttggtgaagt
      961 catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg
     1021 taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga gctggcacct
     1081 ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc gtcctgcgcc
     1141 aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg gtgataagcc
     1201 gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg gtccgttcgc
     1261 tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt
     1321 ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg aaaaagccta
     1381 acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat tggaatacaa
     1441 tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata tcacggggag
     1501 cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa agtacaagcc
     1561 tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt aggtccggct
     1621 ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc aaaaactgat
     1681 tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga ccgttctttc
     1741 actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc tggtatcaag
     1801 tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag
     1861 gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc gatgactcgc
     1921 tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga aatggctaaa
     1981 ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca gtacgacatc
     2041 aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga aagcgtggac
     2101 gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg tggtgcaact
     2161 gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca aggtgcaaac
     2221 gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct ggctgaccag
     2281 atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt
     2341 gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc gaaagtgggt
     2401 actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca ggttcgttac
     2461 cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt tgacgctgac
     2521 aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa accgactcag
     2581 gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc aggtgttgca
     2641 gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg cggtgatttt
     2701 gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga gatattcggg
     2761 ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg tgactggtgc
     2821 gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa gatttgttcg
     2881 ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca agaaacttaa
     2941 tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg gcttgtttct
     3001 gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc cagagatttt
     3061 ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca aacaagcgat
     3121 tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg cggattcccg
     3181 tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag ctggcgtata
     3241 catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc cgttcgagtg
     3301 cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac acccgggcgc
     3361 tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg tcaaagccgc
     3421 tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa ctctgccgac
     3481 ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg ctggtaaact
     3541 ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc agttatcttt
     3601 ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt aaatgtctat
     3661 cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg ttgtagaact
     3721 gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag ctgtagctgc
     3781 tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga aagctgctgg
     3841 cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg gtctgaaaga
     3901 agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga gcaaagacga
     3961 cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag ttaaataagc
     4021 caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt ttggtcacca
     4081 gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact gctgtgcctt
     4141 tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt tctgtgtaaa
     4201 tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt tccattttcc
     4261 ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt cgacttgtca
     4321 gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt
     4381 ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt
     4441 ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt
     4501 ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc
     4561 ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac
     4621 cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa
     4681 aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta
     4741 cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg
     4801 tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc
     4861 gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt
     4921 tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact
     4981 acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata
     5041 acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca tctttcgaca
     5101 tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc
     5161 gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac atcgcaggta
     5221 aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca
     5281 tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag cgtattgaaa
     5341 cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta cgtgtcgacc
     5401 caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc
     5461 cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt
     5521 atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg
     5581 aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata
     5641 tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt
     5701 ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg
     5761 tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac atgatcaacg
     5821 ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta
     5881 tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc
     5941 cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcatt
     6001 acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctt
     6061 tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaggtga
     6121 ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa ggcaactacg
     6181 ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa gacctggtaa
     6241 cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac tacatggacg
     6301 tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg
     6361 acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg actctgcgcg
     6421 ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa
     6481 ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca
     6541 aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca
     6601 aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg
     6661 aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg
     6721 cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact
     6781 ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg
     6841 aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc gctgacatcc
     6901 cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tacattggtg
     6961 cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt gaaactcagc
     7021 tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag
     7081 actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta
     7141 ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac
     7201 aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg ttcagccgta
     7261 tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg
     7321 atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg gaacagctgg
     7381 ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgca
     7441 aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtgtatctgg
     7501 cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg
     7561 taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag
     7621 acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa
     7681 cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc
     7741 agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg
     7801 ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa
     7861 acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa
     7921 ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg
     7981 gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac
     8041 tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta
     8101 ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag
     8161 tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc accgttaagt
     8221 ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc aaccatcaga
     8281 tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta
     8341 tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct gtcggggtaa
     8401 aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag
     8461 tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg
     8521 ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac
     8581 cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa
     8641 gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag
     8701 aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa
     8761 ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg
     8821 ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt
     8881 atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg
     8941 gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga agcaatccag
     9001 gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga agagctgaac
     9061 gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact gctggaagcg
     9121 ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg
     9181 ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac
     9241 gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct
     9301 gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt agacgccctg
     9361 ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc tctgaaatct
     9421 ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct cggtaagcgt
     9481 gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc
     9541 ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg caagctggaa
     9601 ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct
     9661 gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca
     9721 ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct
     9781 atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg
     9841 gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct
     9901 accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt
     9961 gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg
    10021 ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg
    10081 cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt agtagcgaaa
    10141 accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt
    10201 ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac
    10261 acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac
    10321 accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga catggtcatc
    10381 ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag
    10441 cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg
    10501 gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtg
    10561 attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc
    10621 gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg
    10681 atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt
    10741 ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc
    10801 gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac
    10861 ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc
    10921 gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa
    10981 gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct gctgcacgaa
    11041 cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg ttctgttgta
    11101 tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc
    11161 cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg
    11221 ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct
    11281 gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt
    11341 gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa actgatcgac
    11401 gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact ggcgaaaggc
    11461 gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg
    11521 gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg ccagaccatt
    11581 actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga ttccgcagaa
    11641 cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac
    11701 gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg taaagcgatt
    11761 gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg tattccgcag
    11821 gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa
    11881 gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt ttccttcggt
    11941 aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag cgatccgtac
    12001 gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt
    12061 ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt
    12121 catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgtt
    12181 aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc
    12241 gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag
    12301 atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg
    12361 ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag
    12421 gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc
    12481 ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac
    12541 caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca
    12601 gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac
    12661 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatgggtg
    12721 ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga aacgaggaag
    12781 aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa acacaacgct
    12841 ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta cgttcttaca
    12901 ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct ttccataccg
    12961 gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga tcgtcgtgcg
    13021 gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc gttttcgaga
    13081 aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac ggtgattccc
    13141 gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag atggtttgca
    13201 ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac gggcgcaggc
    13261 gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat agcaaatgtt
    13321 cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc gcgccgaggc
    13381 ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc cagaagaagt
    13441 cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat gtctcctgat
    13501 aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc tccgccagcg
    13561 gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga tgacgacgaa
    13621 agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga tgttcaaagc
    13681 ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc gttttgcgct
    13741 tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa agattggaaa
    13801 gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc agacgctgcg
    13861 cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc gccatcatat
    13921 cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc gttttgccgt
    13981 tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg aaggttttca
    14041 tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat gactgcggct
    14101 gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa atgccttcgc
    14161 catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg cgtcggcccc
    14221 catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga caaccaccgg
    14281 aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc cctgattcga
    14341 gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca gacgtttaca
    14401 caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta ccagcatttc
    14461 ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag ggtgaatctc
    14521 taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga aaatggcttc
    14581 ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg cgataagcgg
    14641 ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca gtgtcaccag
    14701 ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga atttccctgt
    14761 gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct caaccccctg
    14821 caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc gtccactgct
    14881 cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt tggtcgagtt
    14941 gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga tcgttaaaca
    15001 ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa cgccaggctg
    15061 cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt ctctataccg
    15121 cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac caccgggcca
    15181 accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca caggcagcgg
    15241 taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa tccgaccgcg
    15301 ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc ctggcgagtc
    15361 gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc ttttaacgcc
    15421 tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa ctgtgtcagt
    15481 cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa gaggatttgt
    15541 cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt cccgacgcca
    15601 gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat aatcagcacc
    15661 tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag aatttggcgg
    15721 ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca gcaactgtgc
    15781 cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga tactgccgac
    15841 acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg ccacggtggg
    15901 ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg gtgcagaagg
    15961 catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg ggcgtgctgc
    16021 cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca ggcctgccgc
    16081 gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga cgccatacgc
    16141 ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt tatagcggcg
    16201 gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc gatctttgat
    16261 gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact gtacgctgtc
    16321 caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct gatacatcac
    16381 gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc cgacatatcc
    16441 gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg gctgattttc
    16501 atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc tgactcttgc
    16561 ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag attaaactgg
    16621 tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat ttgcgcgcct
    16681 ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat aagcccctgc
    16741 ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta acagagcatc
    16801 gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg gtcatagccc
    16861 ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg gcagtgctct
    16921 aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg gccttcaatc
    16981 atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt atgcagctcg
    17041 gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc gtcgcccagc
    17101 gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg ttgatagagg
    17161 aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc gcgagagacg
    17221 ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc cgcatggata
    17281 gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa cgcttcccag
    17341 gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg cactgtaccg
    17401 atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata gcgaccggtg
    17461 gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt ttccacttct
    17521 tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt taccaggaaa
    17581 ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg gataatcgca
    17641 cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg cagatgtgcg
    17701 ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg ctcgcggccc
    17761 atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg gcgggcgtag
    17821 tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa gccgctaaaa
    17881 cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga actgcgcacg
    17941 gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt tgccagccct
    18001 tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc gtagaccgga
    18061 atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag cgtcgggcta
    18121 agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat ataaatgcgt
    18181 tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc ccgggcgcgt
    18241 tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc aaggacgtgg
    18301 cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg atagcagatt
    18361 aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc gaataaacgg
    18421 tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg cgttgaatgt
    18481 aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg gcgtcaagca
    18541 ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg ccgatgtcag
    18601 ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc aggctggaat
    18661 cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc ttcgcggcgc
    18721 gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata ctgaaatgtc
    18781 cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt tcgtttagcc
    18841 tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc acgagcagat
    18901 gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg cgctccgtca
    18961 ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa actgctcagt
    19021 gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca agagctatgg
    19081 atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa caaaaattat
    19141 ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc
    19201 gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa cagcagcggc
    19261 gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg tttcaactgg
    19321 ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt ggttactgcg
    19381 ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc
    19441 gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc
    19501 tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc ggattcgtcg
    19561 aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag agcggaatta
    19621 aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag tctttaatga
    19681 ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa gaattgctcg
    19741 aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc accgtagcgc
    19801 gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga tgatacactg
    19861 accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat cgttatctgc
    19921 gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc caggcgggtc
    19981 gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg tcgctgtgca
    20041 aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac ccgctggatg
    20101 cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta gggctctatt
    20161 ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc gacgtcgata
    20221 aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca
    20281 ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc ccgtggacgc
    20341 tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc aaaaaaatga
    20401 tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa agcgtcactt
    20461 tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc gacacctggg
    20521 gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg cataaaattg
    20581 ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg tttaccaaag
    20641 gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg ggcctcgact
    20701 ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg ttgcagggta
    20761 atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa gtagcgacta
    20821 tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac ggcattcatc
    20881 aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga ctgtctgaac
    20941 aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa caaatcgaac
    21001 tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat ctgatcgccg
    21061 gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg gtgctgctga
    21121 aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc accatgcctt
    21181 acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg tgggagatgc
    21241 tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc
    21301 ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc gtggcgaaaa
    21361 aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg gccccactga
    21421 tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt
    21481 ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta caacgctgca
    21541 tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc
    21601 cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg gataatttcc
    21661 gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag cgcctggaaa
    21721 gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc aattacgcca
    21781 tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt ctcgatctca
    21841 tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa ctggagaaat
    21901 ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat ccggcaatcg
    21961 atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa acgctcgaac
    22021 acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa atgactcagg
    22081 ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag caagaatggg
    22141 acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat atcgagctga
    22201 taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt ataatttttc
    22261 agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga gtcttccctt
    22321 tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg
    22381 caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg ataaacacat
    22441 tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag aaagcagaac
    22501 tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt actgagtctc
    22561 tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg aaccaccgcg
    22621 ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg
    22681 taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat tgcgtggcag
    22741 tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt cgatcattga
    22801 tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt cctcccttta
    22861 ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg
    22921 ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc acgacgcgag
    22981 agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac agcaaaccgc
    23041 cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg caacgtgaat
    23101 ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt tatcgtgctg
    23161 atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg cgccaggggc
    23221 gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt aaacctgatt
    23281 tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct gttgatgtca
    23341 gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca aactctgatt
    23401 tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca tacccatatg
    23461 accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg gaatacccgc
    23521 ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac gcaaattctc
    23581 catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg ctaacagggc
    23641 attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa
    23701 gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg
    23761 ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg ccattgctga
    23821 aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc ctccagtggt
    23881 tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata tgacgggtaa
    23941 tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt catttttact
    24001 cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa gatggcatga
    24061 tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat gcaacgttct
    24121 aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt tgggctggta
    24181 gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc cgccagacaa
    24241 acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc gcgcgtcggc
    24301 atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat ggccgggcag
    24361 cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat gcatagcaac
    24421 tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt acatccggga
    24481 gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct ggaaatttat
    24541 cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat gcagcagtat
    24601 gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt gagtgccgaa
    24661 gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt cttgctggca
    24721 agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct tctacaagat
    24781 gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg cgttgcccac
    24841 gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc cgagcgcgcg
    24901 cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc cgaccgttta
    24961 aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc tttgcaggcg
    25021 gtggatctca acacgctgat taaccactca ttacagctgg taagccagga tgcgaacagc
    25081 cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca ggccgatccg
    25141 gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc gattggtcag
    25201 catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat cagcgttacc
    25261 gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc gtacttcacc
    25321 actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt tgaacaacac
    25381 ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac cctctggctt
    25441 ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc gatattctgg
    25501 tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc ggctggggct
    25561 ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt
    25621 ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc acgctaaaag
    25681 agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac tccagcgtcg
    25741 agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag ccgctggatt
    25801 tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt attgacgctg
    25861 aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg gcgatgcaac
    25921 acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg atccacggcg
    25981 attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc gcacgtagcg
    26041 aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg gaatctgaat
    26101 tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa gggcgctttg
    26161 ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc
    26221 aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt agcaaccaga
    26281 ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc gcagaggtga
    26341 atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg attgaagtac
    26401 catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt cttcagcgct
    26461 ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg gatttgctga
    26521 tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa cgggcggtgg
    26581 tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt gccggtacgc
    26641 cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa aaagaagtga
    26701 ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt cagttaggga
    26761 tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt tcgatagcgc
    26821 gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc attaaggcat
    26881 aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac agtacgcgcc
    26941 cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg aacactttgc
    27001 cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg
    27061 gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac tctgacgttt
    27121 tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg gacttcatgc
    27181 gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg ataaccttcg
    27241 gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag gtgttgcctt
    27301 ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc tgatgaactt
    27361 catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt ggtccggtat
    27421 ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca tgctcgccgt
    27481 ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata acgatgcgat
    27541 gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct tcggcttctt
    27601 ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct ttaatgacga
    27661 tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg aagttctggt
    27721 attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct ttcgaacctt
    27781 ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg cggaaggtat
    27841 cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca atcttttcgt
    27901 tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg ttttgcagcg
    27961 cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca accagcggcg
    28021 actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc actaatactt
    28081 tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc
    28141 gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag gctggattac
    28201 gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga agaacgcgtc
    28261 agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa taccggcgat
    28321 tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca tattgttttt
    28381 ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga acagcgcatc
    28441 acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt ccgcaccgac
    28501 catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga aatcaagacc
    28561 cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct tggcggcggt
    28621 gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa actgacgaga
    28681 aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga tgccgccgaa
    28741 tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg aattgccgat
    28801 agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg cgaactcttt
    28861 cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg ctttaccctg
    28921 aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat agaaggcagc
    28981 ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag
    29041 cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg gaaccatgct
    29101 gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg ctttgatggc
    29161 gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct ctttaataat
    29221 ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct tagcggcgga
    29281 gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca gcgagcagcc
    29341 ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat cgataggctg
    29401 gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa tgccaccatg
    29461 tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc
    29521 ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc cccctgtaga
    29581 cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc
    29641 agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa atcccctgga
    29701 tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct aacgccccta
    29761 acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa
    29821 tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa aaatacagaa
    29881 attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg ggttttgctg
    29941 gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc ggagaaatcc
    30001 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg
    30061 gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg
    30121 ccacctcgcg acagtgcgct aaagcgcgtc gcaactgctc tttaacaatt tatcagacaa
    30181 tctgtgtggg cactcgaaga tacggattct taacgtcgca agacgaaaaa tgaataccaa
    30241 gtctcaagag tgaac
//
LOCUS       DAEEBB010000040        30001 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEEBB010000040 DAEEBB010000000
VERSION     DAEEBB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.040"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            38..580
                     /locus_tag="KLF07_001673"
     CDS             38..580
                     /locus_tag="KLF07_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831710.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="KLF07_001674"
     CDS             663..1364
                     /locus_tag="KLF07_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD9831711.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="KLF07_001675"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="KLF07_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="KLF07_001676"
     CDS             3979..5049
                     /locus_tag="KLF07_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831712.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="KLF07_001677"
     CDS             5061..5603
                     /locus_tag="KLF07_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831713.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="KLF07_001678"
     CDS             5611..6126
                     /locus_tag="KLF07_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD9831714.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="KLF07_001679"
     CDS             6092..6829
                     /locus_tag="KLF07_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD9831715.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="KLF07_001680"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="KLF07_001680"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBD9831716.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="KLF07_001681"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="KLF07_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBD9831717.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="KLF07_001682"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="KLF07_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBD9831718.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="KLF07_001683"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="KLF07_001683"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBD9831719.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="KLF07_001684"
     CDS             12136..14043
                     /locus_tag="KLF07_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9831720.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="KLF07_001685"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="KLF07_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBD9831721.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="KLF07_001686"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="KLF07_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBD9831722.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="KLF07_001687"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="KLF07_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBD9831723.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="KLF07_001688"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="KLF07_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBD9831724.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="KLF07_001689"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="KLF07_001689"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBD9831725.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="KLF07_001690"
     CDS             complement(18711..20471)
                     /locus_tag="KLF07_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBD9831726.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="KLF07_001691"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="KLF07_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBD9831727.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="KLF07_001692"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="KLF07_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBD9831728.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="KLF07_001693"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="KLF07_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBD9831729.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="KLF07_001694"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="KLF07_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBD9831730.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="KLF07_001695"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="KLF07_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBD9831731.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="KLF07_001696"
     CDS             complement(26072..26518)
                     /locus_tag="KLF07_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBD9831732.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="KLF07_001697"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="KLF07_001697"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBD9831733.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="KLF07_001698"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="KLF07_001698"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBD9831734.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="KLF07_001699"
     CDS             complement(29281..29943)
                     /locus_tag="KLF07_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBD9831735.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7732 a         7083 c         7879 g         7307 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagttaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcagatggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgt cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 c
//
LOCUS       DAEEBB010000041        29548 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEEBB010000041 DAEEBB010000000
VERSION     DAEEBB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.041"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="KLF07_001700"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="KLF07_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HBD9831736.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="KLF07_001701"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="KLF07_001701"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBD9831737.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="KLF07_001702"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="KLF07_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBD9831738.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="KLF07_001703"
     CDS             complement(3644..4030)
                     /locus_tag="KLF07_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBD9831739.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="KLF07_001704"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="KLF07_001704"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HBD9831740.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="KLF07_001705"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="KLF07_001705"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBD9831741.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="KLF07_001706"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="KLF07_001706"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBD9831742.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="KLF07_001707"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="KLF07_001707"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBD9831743.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="KLF07_001708"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="KLF07_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBD9831744.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="KLF07_001709"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="KLF07_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBD9831745.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="KLF07_001710"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="KLF07_001710"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBD9831746.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="KLF07_001711"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="KLF07_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBD9831747.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="KLF07_001712"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="KLF07_001712"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBD9831748.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="KLF07_001713"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="KLF07_001713"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBD9831749.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="KLF07_001714"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="KLF07_001714"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD9831750.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="KLF07_001715"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="KLF07_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBD9831751.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="KLF07_001716"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="KLF07_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBD9831752.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="KLF07_001717"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="KLF07_001717"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBD9831753.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="KLF07_001718"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="KLF07_001718"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBD9831754.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="KLF07_001719"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="KLF07_001719"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBD9831755.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="KLF07_001720"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="KLF07_001720"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBD9831756.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="KLF07_001721"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="KLF07_001721"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBD9831757.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="KLF07_001722"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="KLF07_001722"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBD9831758.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="KLF07_001723"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="KLF07_001723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBD9831759.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="KLF07_001724"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="KLF07_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBD9831760.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAEEBB010000042        28804 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEEBB010000042 DAEEBB010000000
VERSION     DAEEBB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.042"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(396..602)
                     /locus_tag="KLF07_001725"
     CDS             complement(396..602)
                     /locus_tag="KLF07_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBD9831761.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            915..1004
                     /gene="kdpF"
                     /locus_tag="KLF07_001726"
     CDS             915..1004
                     /gene="kdpF"
                     /locus_tag="KLF07_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBD9831762.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1004..2677
                     /gene="kdpA"
                     /locus_tag="KLF07_001727"
     CDS             1004..2677
                     /gene="kdpA"
                     /locus_tag="KLF07_001727"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBD9831763.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2700..4748
                     /gene="kdpB"
                     /locus_tag="KLF07_001728"
     CDS             2700..4748
                     /gene="kdpB"
                     /locus_tag="KLF07_001728"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBD9831764.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4757..5329
                     /gene="kdpC"
                     /locus_tag="KLF07_001729"
     CDS             4757..5329
                     /gene="kdpC"
                     /locus_tag="KLF07_001729"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBD9831765.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5322..8006
                     /gene="kdpD"
                     /locus_tag="KLF07_001730"
     CDS             5322..8006
                     /gene="kdpD"
                     /locus_tag="KLF07_001730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBD9831766.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8003..8680
                     /gene="kdpE"
                     /locus_tag="KLF07_001731"
     CDS             8003..8680
                     /gene="kdpE"
                     /locus_tag="KLF07_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBD9831767.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8955..9059
                     /locus_tag="KLF07_001732"
     CDS             8955..9059
                     /locus_tag="KLF07_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBD9831768.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9370..11568
                     /gene="speF"
                     /locus_tag="KLF07_001733"
     CDS             9370..11568
                     /gene="speF"
                     /locus_tag="KLF07_001733"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBD9831769.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11565..12884
                     /gene="potE"
                     /locus_tag="KLF07_001734"
     CDS             11565..12884
                     /gene="potE"
                     /locus_tag="KLF07_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBD9831770.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12940..14580)
                     /gene="pgm"
                     /locus_tag="KLF07_001735"
     CDS             complement(12940..14580)
                     /gene="pgm"
                     /locus_tag="KLF07_001735"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBD9831771.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14606..15151)
                     /gene="seqA"
                     /locus_tag="KLF07_001736"
     CDS             complement(14606..15151)
                     /gene="seqA"
                     /locus_tag="KLF07_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBD9831772.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15336..16100
                     /gene="ybfF"
                     /locus_tag="KLF07_001737"
     CDS             15336..16100
                     /gene="ybfF"
                     /locus_tag="KLF07_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD9831773.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16240..16533
                     /gene="ybfE"
                     /locus_tag="KLF07_001738"
     CDS             16240..16533
                     /gene="ybfE"
                     /locus_tag="KLF07_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBD9831774.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16673..17203
                     /gene="fldA"
                     /locus_tag="KLF07_001739"
     CDS             16673..17203
                     /gene="fldA"
                     /locus_tag="KLF07_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBD9831775.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17413..17499
                     /gene="uof"
                     /locus_tag="KLF07_001740"
     CDS             17413..17499
                     /gene="uof"
                     /locus_tag="KLF07_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBD9831776.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17492..17938
                     /gene="fur"
                     /locus_tag="KLF07_001741"
     CDS             17492..17938
                     /gene="fur"
                     /locus_tag="KLF07_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBD9831777.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18023..18349)
                     /locus_tag="KLF07_001742"
     CDS             complement(18023..18349)
                     /locus_tag="KLF07_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831778.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18399..19805)
                     /gene="chiP"
                     /locus_tag="KLF07_001743"
                     /pseudo
     CDS             complement(18399..19805)
                     /gene="chiP"
                     /locus_tag="KLF07_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20383..22047)
                     /gene="glnS"
                     /locus_tag="KLF07_001744"
     CDS             complement(20383..22047)
                     /gene="glnS"
                     /locus_tag="KLF07_001744"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBD9831779.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22307..24253)
                     /gene="nagE"
                     /locus_tag="KLF07_001745"
     CDS             complement(22307..24253)
                     /gene="nagE"
                     /locus_tag="KLF07_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBD9831780.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24586..25386
                     /gene="nagB"
                     /locus_tag="KLF07_001746"
     CDS             24586..25386
                     /gene="nagB"
                     /locus_tag="KLF07_001746"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD9831781.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25446..26594
                     /gene="nagA"
                     /locus_tag="KLF07_001747"
     CDS             25446..26594
                     /gene="nagA"
                     /locus_tag="KLF07_001747"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD9831782.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26603..27823
                     /gene="nagC"
                     /locus_tag="KLF07_001748"
                     /gene_synonym="nagR"
     CDS             26603..27823
                     /gene="nagC"
                     /locus_tag="KLF07_001748"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBD9831783.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27871..28623
                     /gene="nagD"
                     /locus_tag="KLF07_001749"
     CDS             27871..28623
                     /gene="nagD"
                     /locus_tag="KLF07_001749"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBD9831784.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6748 a         7348 c         7558 g         7150 t
ORIGIN      
        1 ggggattgac cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg
       61 tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca tactgcgtca
      121 tgtcgccctg acgcgccgcc ggttttccgc tcatttctca tccttgtttt atcgggttat
      181 ttgtccgctg acaggcaaga ccctacagat ttataagaaa gccttaagtg agatatccgt
      241 cagatagaaa caattaggaa aatatttata tatatttcat gaatatatgt ttcgattggt
      301 ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg ccagcgtttc
      361 cgctggcggt tttcaatgta ttcagaactc agcaatcagt aaaaatcacc ggttgccttt
      421 tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg gtgcaggtag
      481 ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag aacgcccgct
      541 gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct gtagagttcc
      601 atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat gaatttgttt
      661 atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac cacttatttt
      721 tgcttattga tggtttattt acattcactc tgtaattaag ttacacaaaa gttaaattaa
      781 tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta caccccgccc
      841 gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca cttttaggcc
      901 actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg tttttattac
      961 tgggttattt ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt
     1021 cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtcctt taggcagcgg
     1081 gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg aacgtatact
     1141 ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc tttgtgccat
     1201 tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg gtcagcacta
     1261 tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc tgaataccgc
     1321 cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca cgttgagcta
     1381 tttcagccag atggcaggct taacggtaca aaactttctt tctgccgcca gcgggattgc
     1441 ggtgattttt gccttcatcc gtgcgtttac tcgccagagc atgagcacac tcgggaatgc
     1501 ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc
     1561 actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg cagtgaatac
     1621 cgttgaagga gcgcaacagc tgttacccat gggaccagta gcttctcagg aagcgatcaa
     1681 gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa
     1741 cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc caacagcgct
     1801 gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc
     1861 gatgtcagtg atttttgtca tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa
     1921 tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta aagagagccg
     1981 tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc
     2041 ggtgattgcg atgcatgatt cgtttaccgc tctcggcggc atggtgccga tgtggctaat
     2101 gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca tgatgctgtt
     2161 tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg aatatctggg
     2221 taaaaaaatc gacgtacgcg agatgaaact gactgcactg gcgattctgg ttactccaac
     2281 actggtactg atgggcgcgg cgttggcgat gatgaccgac gccggacgta gcgccatgct
     2341 taaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa
     2401 caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact gtttactggc
     2461 gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa ttgccggttc
     2521 gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc acggcccgct
     2581 gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct ttatccctgc
     2641 cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagcga gcactgaata
     2701 tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg ctgaaagaag
     2761 cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga
     2821 tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg atgcctggca
     2881 atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg ttcgctaatt
     2941 tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt aaaggggtga
     3001 aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg gcggacaaag
     3061 ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc gatattatcc
     3121 cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg
     3181 aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc
     3241 gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag acatttctgg
     3301 atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac gagattgccc
     3361 tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc acgctgtggc
     3421 cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg ctgctggtct
     3481 gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc gggatgagtc
     3541 ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg gcaggtgacg
     3601 ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt caggcgtcgg
     3661 agtttatccc cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca caactggctt
     3721 cgctggctga tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag caacgtttta
     3781 acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa
     3841 gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct gtcgatgcca
     3901 ttcgccgcca tattgaggct aacggcggtc acttccctgc cgatgttgat caaaaagtcg
     3961 atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct cgtgtgctgg
     4021 gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc gcccagctgc
     4081 gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     4141 tagctgcgga agcgggtgtc gatgattttc tcgccgaagc aacaccggag gccaagctgg
     4201 cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc gacggcacca
     4261 acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc ggcacccagg
     4321 cggcgaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag ttgatcgagg
     4381 tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     4441 ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca acatatccgc
     4501 agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg
     4561 tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt
     4621 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     4681 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc gtttgcggtc
     4741 tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc tctttctgtt
     4801 attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat ggtggtttcc
     4861 ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcgtggct cggcattaat
     4921 cgggcagaat tttaccggca acggctattt tcatggtcgc ccgtcagcaa cggctgaaat
     4981 gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc ctgagctgga
     5041 taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg ccagcgcgag
     5101 cattccggtt gaactggtga cggcatcggc aagcgggctg gacaataata tcaccccgca
     5161 agcggcggcc tggcagatcc cacgcatcgc gaaagcgcgt aatctcagcg ttgaacagct
     5221 cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg gccagccggt
     5281 cgtcaacatt gtcgaactca atctggcgct ggataagctt gatgaataac gaacccttac
     5341 gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     5401 ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     5461 gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac gggcgaaaag
     5521 ataccgccgc aatgctggaa gggctggctg ttctgccgcc aaaacgcctg gcgtaccgtg
     5581 ggcggcacat ctgcgagttt gacctcgatg ccgccctcgc ccgccgtccg gcgctgatct
     5641 taatggacga actggcgcac agtaatgcgc caggttctcg tcaccctaaa cgctggcagg
     5701 acatcgaaga gctgctggaa gcaggcattg atgttttcac taccgtcaac gttcagcatc
     5761 tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc
     5821 ccgatccttt tttcgatgcc gccgacgacg tggttctggt ggacttgccc ccggacgatc
     5881 tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag cgcgccattg
     5941 aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg cgccgtactg
     6001 ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctagcgaa gagaaagtct
     6061 ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggtagt gagaagctgg
     6121 tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt ctggcacgcg gtgtatgttg
     6181 aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg tgcaattctc agcgccttac
     6241 gtctggcgca ggaactgggc gcggagacgg caacactttc tgatccagcg gaagagaaag
     6301 cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt cgcccggcct
     6361 cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc gcccccgatc
     6421 tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac aacgcgccgg
     6481 atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg gttgccgccg
     6541 cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt gatgccgcca
     6601 acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat ggacgctggc
     6661 cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt atcgccccac
     6721 gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg atgttaaccg
     6781 tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg gtagcccgtt
     6841 accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg gtgggccgca
     6901 gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt catgcccgca
     6961 gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat ccgcaaggaa
     7021 tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg cctgcgggcg
     7081 cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa agcggcgaga
     7141 aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg atcccggaac
     7201 agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcccttgag cggctgacgc
     7261 taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     7321 tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt ggtcaggcag
     7381 aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag gccagcgaga
     7441 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     7501 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     7561 gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     7621 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     7681 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cactttgagg
     7741 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca ggccaggagc
     7801 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg ggtgtcgggc
     7861 ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt accgcgttca
     7921 accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc gctcctgaac
     7981 ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     8041 tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg aggccgaaac
     8101 tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta ttctcgatct
     8161 cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcccggt
     8221 gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg cgctggatgc
     8281 cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg cccgtctgcg
     8341 cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga aattttccga
     8401 tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac
     8461 accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag tactcaccca
     8521 gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc actatttgcg
     8581 tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc ctcgccactt
     8641 cattactgaa accggtattg gatatcggtt tatgctttga attgttattc aataaattcg
     8701 cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt ggcaggttta
     8761 atttcttaac gcaaatataa aacacgaaat tacaacattt aagtaacaaa acagcatcta
     8821 atatggcgat attcataatt aatgatttat tatctggggt atgatcgttt tttgttgatc
     8881 ttcttcacag attatagcca tttcatggat agaataactc taccttcaac tgacacagca
     8941 agaggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg cggacaaccc
     9001 atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat gcccgctagt
     9061 cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat tacaggttaa
     9121 tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa taattcgagt
     9181 cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg gtctgggtta
     9241 ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt tgcttttttt
     9301 ccggcgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg aaataaagag
     9361 atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga ttgtttttcc
     9421 actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc cgccattatt
     9481 ttatcagtca atgatgttga acgtggaaaa ctcgatgaaa tagacgctac tggctatggc
     9541 attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata tctgccacgt
     9601 atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg tcagttagaa
     9661 accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc actggtcgat
     9721 tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg tgagtttttc
     9781 cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct gttccgttcc
     9841 gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg cgcgccatgc
     9901 attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt cgttctaaat
     9961 ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg tgatctggtg
    10021 ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca ggctggtgca
    10081 acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg cattgatgcg
    10141 cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca
    10201 aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga cggtacgatt
    10261 tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat tctgtttgac
    10321 tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc gccgctgttg
    10381 ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca taaacagcag
    10441 gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa agggcaacag
    10501 cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcacgcctc caccagcccg
    10561 ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg tgtcagcggt
    10621 cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaaact gatcctcgat
    10681 aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc ctggcagtcg
    10741 tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc aggggaacac
    10801 tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccttg caaactgttg
    10861 ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg
    10921 acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac
    10981 tccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca acttgttgcc
    11041 ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga agtgctgcct
    11101 tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca gttgtgccag
    11161 gaaatgcatg atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga gatgttccgc
    11221 aaagcgcact tcccacacgt cagcatgaat ccgcaagaag ccaactacgc ctatttacgc
    11281 ggtgaagtgg agctggttcg tctgccggat gccgaaggtc gtatcgctgc cgaaggggcg
    11341 cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg tggtgctgtt
    11401 ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt tgcaccggag
    11461 ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg ctatgtcatc
    11521 aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt caggctaaat
    11581 cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg atgggctccg
    11641 gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt atctcctggc
    11701 tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc ggtatgttca
    11761 gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc tttcggtaaa tccggtaact
    11821 ttatggcgaa ctatacctac ggcgtctcac tgctgattgc taacgtcgcg attgctattt
    11881 cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg cagattggtc
    11941 ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt gcgcgcatta
    12001 ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta ggcctgtgca
    12061 ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat ccgcatcatg
    12121 caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct tttctcggtc
    12181 tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat gtgccaatcg
    12241 cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc aacgtgattg
    12301 cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc ctggccttcg
    12361 cacagatgtt cacaccagaa gtgggtaaag tcattatggc gctgatggtg atgtcctgct
    12421 gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaagtct tcatctgatg
    12481 aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg gtgcagggaa
    12541 tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc ccgtcgctaa
    12601 acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt ccgtatattc
    12661 tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc tcaaaagcga
    12721 aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg ctgtactcat
    12781 ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg acactgtatg
    12841 gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc aaagtaaaaa
    12901 agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt tcagaacttc
    12961 gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac cgaggaagct
    13021 ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga accagccgtt
    13081 gtcagtcatc actttcagac cgccgataga agcaccattg cccggtgccg ccgtcaggcg
    13141 cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag acagcttaga
    13201 cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc tcggcgcacc
    13261 aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga cagcggtgat
    13321 ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg gtggaccacg gcgtgccgtc
    13381 gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc tgccgtcgaa
    13441 cagaccatca acaaaccatt tgaaacctac cgggacttct accagcttgc ggcccaagtc
    13501 attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag caacatcttt
    13561 gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt agtggttcgg
    13621 attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt cgttagcaaa
    13681 cgccagatcg aacttatcac gcaatgccag caggcccgcc atcgcacact cggaggagca
    13741 gtccatacgg atcgcgccgt ctttatcgag gtgcataaag cggaaggttt gatcgacctg
    13801 atcgttaacg atggtcaggt tgaggttgta atactcgcca attcgcttcc agtattcgat
    13861 accggaacca cccagcggat caacgcccag cgtcaggccc gttttctgaa tcgccgccat
    13921 atcaacgata tcggccagac cttccacaaa ctgctgcacc agatcctgct ctttcacatg
    13981 accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc catcggccat
    14041 cagtgcgttg gcacgatctt ccaccacttt agtgacgttg gtatcagccg ggccaccatt
    14101 tggcggattg tacttgatac caccatcttc cggcgggtta tgggagggtg taatcacgat
    14161 accgtctgcc agcgggccac cttttttatt atgaaccagg atggcattgg aaacggcagg
    14221 cgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg ctgccagcac
    14281 ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac ccacatagca
    14341 agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga tcgccagaat
    14401 gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag taccgaattt
    14461 caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg ccgtcagttg
    14521 ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt ggattgccat
    14581 tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca atcaattccg
    14641 ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg ccggtgttgg
    14701 tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc tgattaccat
    14761 ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg tgcaacgatt
    14821 ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac agcagcataa
    14881 agcgattgac cgctcgcttt tgctctgcgt attcgtcaga aagcagaagt tcacgcattg
    14941 cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca ggtgacgcaa
    15001 cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat ttcaacatac
    15061 gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca atatagctgt
    15121 agagttcatc atcaacttca atcgttttca tcttaatcca gtacagtgtc ttagctgaat
    15181 acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag cctggcttgc
    15241 agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt caacataata
    15301 ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc cgcgcgcaaa
    15361 ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg tttggtagcc
    15421 tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc atccaggttg
    15481 atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg gcgatggcgc
    15541 aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt atcggtcact
    15601 ccatgggcgg taaagcggta atggcactta cggcactggc ccgcgatcgc atcgataaac
    15661 tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat gagattttcg
    15721 cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca gcagcaataa
    15781 tgcaccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct tttgttgacg
    15841 gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt gtgggttggg
    15901 agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat tctccgtatg
    15961 ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg gcgcatgtga
    16021 ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta cgcgctatcc
    16081 gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt cgctttccgc
    16141 gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc catccgggca
    16201 gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga acaaacggac
    16261 cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa aactaatccg
    16321 ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg cgacaaagta
    16381 cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc gctgaacgag
    16441 ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt aatgcagcaa
    16501 ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta gcacagtgtg
    16561 cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt gcctttatcc
    16621 gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac tcatggctat
    16681 cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa aaatgattca
    16741 aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca gcaaagaaga
    16801 tctggaagct tatgacattc tgctgctggg catcccaacc tggtattatg gcgaagcgca
    16861 gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg gcaaactggt
    16921 tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg acgcattggg
    16981 caccatccgc gacatcattg aaccgcgcgg tgcaacgatc gttggtcact ggccaactgc
    17041 gggctatcat ttcgaagcat caaaaggtct ggctgatgac gaccactttg tcggtctggc
    17101 tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat gggttaaaca
    17161 gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg cggcgtagac
    17221 tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca aatttgtaac
    17281 ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt tgtaaatgta
    17341 agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc atttaggcgt
    17401 ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc ttctaatgaa
    17461 gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg ccctaaagaa
    17521 agctggcctg aaagtaacgc ttcctcgttt aaaaatccta gaagttcttc aggagccgga
    17581 caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg gtgaagaaat
    17641 tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta tcgtcacccg
    17701 ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc accacgatca
    17761 cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca tcgaagcgcg
    17821 tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc tctatcttta
    17881 cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag gcaaataagc
    17941 cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg gaaataagaa
    18001 aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc caaactttat
    18061 cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga tactgctgat
    18121 aatccagtac gcggacactt tcctggtcgg caaaatgctg atgttgtttc tctttcttca
    18181 gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt gcggtgttga
    18241 tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt gattgcgcac
    18301 aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata gcaaattcct
    18361 caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt gaatggtgcg
    18421 attaccataa atttcacgtc acgctcatcc tggaagatgt tgccgtaacc accgccccag
    18481 cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat cgtgcctttg
    18541 gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc ttcaatagtc
    18601 cggtttttgt cgtagtacgc atccggattg ctctgctagg tcgcaggttt agcatcccat
    18661 gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt caggtcatac
    18721 atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg gttatcccac
    18781 cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg caggaagtat
    18841 ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac tacgtcagcc
    18901 gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag gtcgttgacg
    18961 ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt taacgggcta
    19021 ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata cccttccgcc
    19081 tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc cccaagggag
    19141 tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat ttccagatgc
    19201 cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc agcatcgccg
    19261 taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa gctccagtgc
    19321 ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca aaccggaccg
    19381 tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc ggaccagtct
    19441 tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga gctgtcgccg
    19501 ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat atcagcagca
    19561 taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt ggttttgtat
    19621 ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata tataccgccg
    19681 gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc cataaagccc
    19741 gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc actaaacgta
    19801 cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac agcgaaaaaa
    19861 taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca gactattttt
    19921 cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga gcaaccgcac
    19981 atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt gcagaagaat
    20041 aaaagcgtga aattccagca agatgaaagc ggttacattt tatttaatta tgttttgtta
    20101 ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag aaaagcagaa
    20161 aaaacgccgc tgaacgcggc gttttggatt gtttgcatat ttcttgccgg atgcggcgtg
    20221 aacgccttaa ccggcctaca aaagcacgca aactcaatat attgcagaga tcatgtaggc
    20281 ctgataagcg tagtgcatca ggcaatttag cgtttgaaac tgtttgtctg ttccttgccg
    20341 gatgcgacgt aaacgcccca tccggcatag cgaaacttaa aattactcgc ctactttcgc
    20401 ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg tagaatggcg
    20461 gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc
    20521 tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga
    20581 caggaaatca tccgcagcac cagggttcgg cacgctgaac agacggtcgt acagacggat
    20641 ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga ctttacgacc
    20701 atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg tggtgatatt
    20761 gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac gcagacgcac
    20821 ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggcgcg
    20881 atcgatccaa atctcaccgc taaacggcac ctggcggcta cccatttccg gtttgttcgg
    20941 atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa ccagtttcac
    21001 cggatcgata accgccattg cgcgcggggc gttttcgttg agatcttcac ggatgcagga
    21061 ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa
    21121 ctcacgaata gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg
    21181 cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga
    21241 catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat
    21301 ggtgatgttg tccagcaccc agtcgtacag gcgacggttg tcctggaact caagcgtaca
    21361 cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg
    21421 gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc ggtacagcac
    21481 cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt
    21541 accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct cttcaacgct
    21601 gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt attcgcggat
    21661 ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata
    21721 ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc
    21781 taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt ttaccgggtt
    21841 agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag
    21901 gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt
    21961 gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt
    22021 agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac
    22081 ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagcgaaa atcgaaaatc
    22141 tcactgagat tatggggatg attgatgaaa aaaaaaagcg gcagagaaga tctccaccgc
    22201 ttttgccagt cccgcgtcat tgttggatgc gcgtcttatc caaccaggac tgagcttttg
    22261 taggcctgat aagacgcgtc aagcgtcgca tcaggcataa agcagattac tttttgattt
    22321 catacagcgg tgtttgaccc gccacaacat tgccctgagc tttaatgatc aagccactga
    22381 aatcgtcgat attgctgcaa accaccgggc taatcatcga acgggcgtta gcgttcaggt
    22441 aatccagatc catttccaga atcggttgcc ctgcgctcac ctgcgcgccc tcttccacca
    22501 gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatgtgg acgacaatct
    22561 ccgcgccttt ttcggtttct aggcagaacg cgtggttggt gttgaagatt ttcacgattg
    22621 tccctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca
    22681 ccgctttgct ggcgaatgct tcgtcagaaa cctgatccag tgccacgaca tcaccggtaa
    22741 tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag
    22801 gagcggcagt tgccggagtt gcttcagctg acgcagctgc taccgggcca cgggcaacga
    22861 ctttcttcat cgcatcgcca atggattctg ctttcgcgcc aacaatcacc tgaatggtct
    22921 gtttgttcag ttttactaca ccagaagcac ccagacgttt acacatcgca tcgttgacgc
    22981 gagctgagtc agccacggta aggcgcagac gggtaataca ggcgtcaatc gctttcaggt
    23041 tgtcagtacc gccaaccgca gcaatatagt tggttgccag ttgagtcaga ccttcttcag
    23101 tattgctgtt agcctcttca gttacgatct cgtcttcttt atcttcacga cccggcgttt
    23161 tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg aagaagacaa
    23221 cacccatcac cagcagcatc cagacgttct ggctggcggc cggcaggtta tacatcaacg
    23281 cgtagtcgat agcccccgca gagaaagaga agccagcatg gatacccagc agcgttgcca
    23341 caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcgga gcaaggaaca
    23401 tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa
    23461 gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg
    23521 ccgcacccgg cagaccgaac atcatgatcg ggaagaagcc ggacatgaac atccccgcgg
    23581 tgccgtcacc agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc gccgcgttgg
    23641 tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga
    23701 tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcctgca gaaacgatcc
    23761 actcgccgcc tgcatggata gcgtgctgta ctggcggcca gacgtaacca aaaatggccg
    23821 ccagcaccag gcagaaaaag ccggtggcaa tcggcacaaa gcgtttgccg ccgaagaagc
    23881 tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac
    23941 cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt
    24001 tggttaacac aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg
    24061 accagctgga tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa atcgcaccgc
    24121 ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagtg
    24181 ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac
    24241 ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt
    24301 tttattttgc tgcaaattgt actgccgatg ttctgtaatc agattgttag atcatctgct
    24361 acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgccttt tgtgagtttt
    24421 gtcaccaaat atcgttatca tcactccctt ttactggcta aagcagaaaa cttattttat
    24481 cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga
    24541 tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc
    24601 ctaactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat
    24661 gcgttcaaac cgactgccga tcgtccgttt gttctgggcc tgccgactgg cggcacgccg
    24721 atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac
    24781 gttgtcacct tcaacatgga cgaatatgtc ggtctgccga aagagcatcc ggaaagctac
    24841 tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac
    24901 cttctcaacg gcaacgcccc ggatatcgac gccgaatgcc gccagtatga agaaaaaatc
    24961 cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca
    25021 tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaaccct gactcatgac
    25081 actcgcgtcg cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc
    25141 ctgactgtcg gtgttggtac attgctggat gccgaagaag tgatgattct ggtgctgggt
    25201 agccagaaag cactggcact gcaggctgca gttgaaggtt gcgtgaacca catgtggacc
    25261 atcagctgtc tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc ttccaccatg
    25321 gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt
    25381 ctgtaattgt tatccctgcc ctggctcctt gctcagggca atatttttta aaatcggggg
    25441 gcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc
    25501 acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc
    25561 cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc
    25621 agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa
    25681 cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc
    25741 ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc gagtacctgg
    25801 caagacatcc gaatcaggcg ttaggtctgc atctggaagg tccgtggctg aatctggtaa
    25861 aaaaaggcac ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc
    25921 tgtgtgaaaa cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg
    25981 aagtcatcag caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga
    26041 cgttgaaaga agcgaaagcc ggtttccgcg cggggattac ctttgccacc catctgtaca
    26101 acgcgatgcc gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgacgaag
    26161 ctgacattta ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc aacattcgta
    26221 acgctaaacg ccttaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag
    26281 gtgccaacat tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt
    26341 gtgtggatga gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta
    26401 atctggtcga acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc
    26461 cggcgcgtgc gattggcgtt gagaaacgtc tcggcacact cgccgcaggt aaagtagcca
    26521 acctgacctc attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg
    26581 tcgtaactca ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga
    26641 tctcgtaaaa cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat
    26701 ctcgcggatt cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac
    26761 gcgtcagctt atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg
    26821 ccgccgcgct atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct
    26881 tggtcgtcac gacgccacca tcaccctgtt tgatctcagc agcaaagtgc tggcagaaga
    26941 acattacccg ctgccggaac gtacccagca aacgctggaa catgccctgt tgaatgccat
    27001 tgctcagttt attgatagct accagcgcaa actgcgcgag ctgatcgcga tttcggtgat
    27061 cctgccaggg cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt
    27121 agaaaactgg gggctggtag aagctctgga agaacgtttt aaagtgacct gtttcgttgg
    27181 tcacgatatc cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga
    27241 agactccatt ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg
    27301 catttttatt ggccgcaacg gtaacgtcgg tgaaattggc catattcagg tcgaaccgct
    27361 gggtgaacgc tgccactgcg gcaactttgg ctgcctggaa actatcgccg ccaacgctgc
    27421 cattgaacaa cgggtgttga atctgttaaa gcagggctac cagagccgcg tgccgctgga
    27481 cgactgcacc atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagtc tggcgtcgga
    27541 agtaattgag tatgtcggtc gtcatctggg taaaaccatc gccattgcta tcaacttatt
    27601 taatccgcaa aaaattgtta ttgccggtga aatcaccgaa gccgataaag tgctgctccc
    27661 tgctattgaa agctgcatta atacccaggc gctgaaggcg tttcgtacta atctgccggt
    27721 ggtacgttct gagctggatc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc
    27781 catgctcaac ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc
    27841 gcttattgtt gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc
    27901 gacggcgtgc tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg
    27961 attatggata aaggcctgcc gctggtgttg ctgaccaact atccttcgca gaccgggcaa
    28021 gatctggcga accgctttgc cactgccggt gtcgatgtgc cggatagcgt gttttatacc
    28081 tctgcgatgg cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc atatgtggtg
    28141 ggcgaaggcg cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac
    28201 cctgatttcg tgattgttgg cgaaacgcgt tcctacaact gggacatgat gcataaagcg
    28261 gcctatttcg tcgctaacgg tgcacgcttt atcgccacca acccggacac ccacgggcgc
    28321 ggtttttatc ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa
    28381 ccgttctatg ttggtaagcc cagcccgtgg atcatccgcg cagcattaaa caaaatgcag
    28441 gcgcattcgg aagaaacggt gattgtcggc gataacttgc gcaccgacat tctggcgggc
    28501 ttccaggcag gtctggaaac gattctggtg ctttctggtg tttcatcgct cgacgatatc
    28561 gacagtatgc cgttccgccc aagctggatt tacccgtcag tcgctgaaat cgacgttatc
    28621 tgaataccac ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca aactgcctga
    28681 tgcgctacgc ttatcaggcc tacgcagctc ctgcaatata ttgaattttc atgcttttgt
    28741 aggccggata aggcgttcac gccgcatccg gcatgaacaa atcgcacttt atcagcaatt
    28801 tggc
//
LOCUS       DAEEBB010000043        28678 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEEBB010000043 DAEEBB010000000
VERSION     DAEEBB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.043"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1046)
                     /locus_tag="KLF07_001750"
     CDS             complement(<1..1046)
                     /locus_tag="KLF07_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD9831785.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="KLF07_001751"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="KLF07_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="KLF07_001752"
     CDS             complement(2433..3281)
                     /locus_tag="KLF07_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBD9831786.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="KLF07_001753"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="KLF07_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBD9831787.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="KLF07_001754"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="KLF07_001754"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBD9831788.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="KLF07_001755"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="KLF07_001755"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBD9831789.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="KLF07_001756"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="KLF07_001756"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBD9831790.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="KLF07_001757"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="KLF07_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBD9831791.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="KLF07_001758"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="KLF07_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBD9831792.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="KLF07_001759"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="KLF07_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBD9831793.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="KLF07_001760"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="KLF07_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBD9831794.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="KLF07_001761"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="KLF07_001761"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBD9831795.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="KLF07_001762"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="KLF07_001762"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBD9831796.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="KLF07_001763"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="KLF07_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="KLF07_001764"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="KLF07_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="KLF07_001765"
     CDS             22669..23652
                     /locus_tag="KLF07_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBD9831797.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="KLF07_001766"
     CDS             23675..25186
                     /locus_tag="KLF07_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD9831798.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="KLF07_001767"
     CDS             25211..26209
                     /locus_tag="KLF07_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9831799.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="KLF07_001768"
     CDS             26242..27336
                     /locus_tag="KLF07_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD9831800.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="KLF07_001769"
     CDS             27348..28220
                     /locus_tag="KLF07_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBD9831801.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="KLF07_001770"
     CDS             complement(28268..>28678)
                     /locus_tag="KLF07_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD9831802.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6817 a         7092 c         7791 g         6978 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgtcgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAEEBB010000044        27803 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEEBB010000044 DAEEBB010000000
VERSION     DAEEBB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.044"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            8..334
                     /gene="tnpA"
                     /locus_tag="KLF07_001771"
                     /pseudo
     CDS             8..334
                     /gene="tnpA"
                     /locus_tag="KLF07_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(465..1788)
                     /locus_tag="KLF07_001772"
                     /pseudo
     CDS             complement(465..1788)
                     /locus_tag="KLF07_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1788..3404)
                     /locus_tag="KLF07_001773"
     CDS             complement(1788..3404)
                     /locus_tag="KLF07_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBD9831803.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3700..4416
                     /locus_tag="KLF07_001774"
     CDS             3700..4416
                     /locus_tag="KLF07_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD9831804.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4413..6074)
                     /locus_tag="KLF07_001775"
     CDS             complement(4413..6074)
                     /locus_tag="KLF07_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBD9831805.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6270..6698)
                     /gene="ibpB"
                     /locus_tag="KLF07_001776"
     CDS             complement(6270..6698)
                     /gene="ibpB"
                     /locus_tag="KLF07_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBD9831806.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6810..7223)
                     /gene="ibpA"
                     /locus_tag="KLF07_001777"
     CDS             complement(6810..7223)
                     /gene="ibpA"
                     /locus_tag="KLF07_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBD9831807.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7217..7289)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7529..7861
                     /locus_tag="KLF07_001778"
     CDS             7529..7861
                     /locus_tag="KLF07_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBD9831808.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7863..9077)
                     /locus_tag="KLF07_001779"
     CDS             complement(7863..9077)
                     /locus_tag="KLF07_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBD9831809.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9178..10242
                     /gene="cbrA"
                     /locus_tag="KLF07_001780"
     CDS             9178..10242
                     /gene="cbrA"
                     /locus_tag="KLF07_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBD9831810.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10239..11576)
                     /locus_tag="KLF07_001781"
     CDS             complement(10239..11576)
                     /locus_tag="KLF07_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9831811.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11651..12799)
                     /gene="dgoD"
                     /locus_tag="KLF07_001782"
                     /pseudo
     CDS             complement(11651..12799)
                     /gene="dgoD"
                     /locus_tag="KLF07_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12796..13413)
                     /gene="dgoA"
                     /locus_tag="KLF07_001783"
     CDS             complement(12796..13413)
                     /gene="dgoA"
                     /locus_tag="KLF07_001783"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBD9831812.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13397..14275)
                     /gene="dgoK"
                     /locus_tag="KLF07_001784"
     CDS             complement(13397..14275)
                     /gene="dgoK"
                     /locus_tag="KLF07_001784"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBD9831813.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14272..14961)
                     /gene="dgoR"
                     /locus_tag="KLF07_001785"
     CDS             complement(14272..14961)
                     /gene="dgoR"
                     /locus_tag="KLF07_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBD9831814.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14930..15562
                     /locus_tag="KLF07_001786"
                     /pseudo
     CDS             14930..15562
                     /locus_tag="KLF07_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15608..16420)
                     /gene="yidA"
                     /locus_tag="KLF07_001787"
     CDS             complement(15608..16420)
                     /gene="yidA"
                     /locus_tag="KLF07_001787"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBD9831815.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16535..16933)
                     /locus_tag="KLF07_001788"
     CDS             complement(16535..16933)
                     /locus_tag="KLF07_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBD9831816.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17173..19587)
                     /gene="gyrB"
                     /locus_tag="KLF07_001789"
     CDS             complement(17173..19587)
                     /gene="gyrB"
                     /locus_tag="KLF07_001789"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBD9831817.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19616..20689)
                     /gene="recF"
                     /locus_tag="KLF07_001790"
     CDS             complement(19616..20689)
                     /gene="recF"
                     /locus_tag="KLF07_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBD9831818.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20689..21789)
                     /gene="dnaN"
                     /locus_tag="KLF07_001791"
     CDS             complement(20689..21789)
                     /gene="dnaN"
                     /locus_tag="KLF07_001791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBD9831819.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21794..23197)
                     /gene="dnaA"
                     /locus_tag="KLF07_001792"
     CDS             complement(21794..23197)
                     /gene="dnaA"
                     /locus_tag="KLF07_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBD9831820.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23494..23574
                     /locus_tag="KLF07_001793"
     CDS             23494..23574
                     /locus_tag="KLF07_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831821.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23804..23944
                     /gene="rpmH"
                     /locus_tag="KLF07_001794"
     CDS             23804..23944
                     /gene="rpmH"
                     /locus_tag="KLF07_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBD9831822.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23961..24320
                     /gene="rnpA"
                     /locus_tag="KLF07_001795"
     CDS             23961..24320
                     /gene="rnpA"
                     /locus_tag="KLF07_001795"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBD9831823.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24284..24541
                     /gene="yidD"
                     /locus_tag="KLF07_001796"
     CDS             24284..24541
                     /gene="yidD"
                     /locus_tag="KLF07_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBD9831824.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24544..26190
                     /gene="yidC"
                     /locus_tag="KLF07_001797"
     CDS             24544..26190
                     /gene="yidC"
                     /locus_tag="KLF07_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBD9831825.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26296..27660
                     /gene="mnmE"
                     /locus_tag="KLF07_001798"
     CDS             26296..27660
                     /gene="mnmE"
                     /locus_tag="KLF07_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBD9831826.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6689 a         7523 c         7093 g         6498 t
ORIGIN      
        1 acgtcagatt tttgactatg acgcgacaga aaaactacgc acttactttt caaatgtatg
       61 tgctgatttt gaagctgaac tggtttaaat ggatggcgaa ccagatcacg tccatttgtt
      121 aattaactat cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcgtatc
      181 gggtaggtta ctgcgacgag atcgaccaga tattgcagtc aggtattact acaaaggcgt
      241 tttgtggagt cctggctatt ttgccaatag ctgcggaggt gcgccaatat ccgtcatccg
      301 ccaatacatt gaacaacagc aaacacctgg ttaggtggaa aaccgcgcct tatatccccg
      361 ccctgaagga cggggtttta cggcgcaccg gataaatttg gttatgcgcc gtcagccgtg
      421 ctaaagaata cggaccagca cccaacggat gccagtccac acaattaacg taactctggc
      481 cagtacgctt tgttggcctc cagcaactca tccagaatag ctttggcgac cgaggcgctc
      541 ggtaccgttt tcgataacgt aatcgcctgc cacaggtgct gatatgaacg ctgttcccag
      601 gcgtccacga ccagtttttc caccgccact tgctgactca ttaacccttt ctgaaactgc
      661 gggatatcgc cgaccaccag tggttctggt ccgttgtgcc cgaccagaca cgggatctcg
      721 accatcgctt catcatcaaa gttatggatt gccccgttgt taggcacaat cagcaacatc
      781 cgctcctgag tgttgaaggc aattgccgcc gccagatcga cgatgtatga cgcatgttcg
      841 tcaatctcca gcttggccgc cgctgaattt cctgccgctg taatggcgcg gcaagcatcg
      901 aaaacctgtt tctcgcgatg ttccattcac ctcattcgcg cgggtatgtt cagggttgga
      961 atgctgaaca acataatccg ggaagagata atatttcaga taggtgttcg gcaatgtatc
     1021 cgggtccagt gcctggacat cccgcgcttt tgcataggtg tcgttccagc tcgcttcagt
     1081 atgtgtatct tgctgcggtg gaacataacc gtgcttcgag acatattgcc gcagctgggg
     1141 catcaggtcg ttgccctgca aatcttcaat tgatgtccac cagccaaagt ggttcaggcc
     1201 gtagtagcgc acgcgcatct gtttgcgatc ttgcagccca acaatttgcg ccatccggct
     1261 ttcaataccg attggcatgt cacagatgtt gaggattttc gcattcgggc gcagacgacg
     1321 cgtggcttct gcgacaatgg ctgccgggtt ggagtagttg agcatccagg catttggtga
     1381 atatttttcc atataatcca ccagttccag gacgccgcca atggaacgca tgccgtacgc
     1441 tattccgccg ggtccgcaag tttcctgacc aacaacaccg tggcgcagtg ggattttttc
     1501 atccagttcg cgcatcgggt acttgccgac gcggatgtgc gccatgacaa aatcaacgtc
     1561 gctgaacgcc acttcaggat cggtggtgta actaaacgca atgtccggcg ctttctcttt
     1621 aaggatgact ttacaggctt cggcaatcac ttcctgccgc gcgccatcgt tatcataaaa
     1681 tttcagtgca cgaagcggga aacgctcctg attcgccagg agcatcaaca cgatgcctgg
     1741 cgtaaaggtg cttccaccgc ctgcgacaac cactgagaat ttggtcatta tactgcctcc
     1801 gtaatggcaa cattttctgc tgattgatga gaattaatta agctatcgag ctgttcacgc
     1861 agctgggata catgcagacc aatgatcacc tgaatggcat cgccactacg gaagacgccg
     1921 tgcgctccca gctttttaaa gacttcgtca tccagcgttt gtgacatgtc atgcagtgca
     1981 atgcgtagac gcgtcgcaca attgttaatg ctggagatat tgccgacccc gcccagggct
     2041 tgcaggatac cggcagcctg atccagctct ttttttggct ctgccgcggt ggtttggcct
     2101 cgcgaggctt tgtattcggc ttttgagtag agtttcactt ccgcatcttc acgtcccggc
     2161 gtgcacatgt tgaactgcag aatcagggtg cggaaaacca cgaagtacag cagggtaaag
     2221 cacaacccaa tggcgatttg ggtcagcatc atatccgcgt ggttgctgaa catcgggatc
     2281 cagttttgcg gtaaaacctg gtcaatcaga cctccgccca tgttgcccac cacaccaaag
     2341 agatacatca cggtcgacat tgaggccgca agcacagcgt gtaccgcaaa cagcaacggt
     2401 gaaatgaaca ggaaggtaaa ttccagcggt tcggtaattc ccaccagcat ggcggttaag
     2461 gttgcgggaa tcagcaagcc cgccactttt acccgatttt ccggtgcggc agtgaagtac
     2521 atcgctaaag aaatgcccac ggcaccaaag atttttgagt taccgtgcag ggcaaaaccg
     2581 ccttccggga acaacgattt cagcggctcg gcgctcaaac tgaactcttg cagatgctgc
     2641 gcccagtaca tctgaatgcc gccttcaaca gctgccggac ccaagataaa cggtccgtag
     2701 atgaagtggt gtaaaccggt tgggatcaga atacgttcga ggaaggtgta aacccagacc
     2761 ccaagcgcac ccgccgaacg caggaacgct tgcagagatt caatccccgt ttgtactttt
     2821 ggccagccga gcaatgtcag ccaggcacag gggatcatca ccaggaaggc gataatcacc
     2881 acataagacg tcccctggaa aatgccgaga aaaaccggca gttttttatc gaacagacgg
     2941 ttatgcagcg ccgtcacaat gccggaaatg ataattgcgc cgataatgct ggtatcgagg
     3001 gttttaatcc cggccatcat tgtcagaccg ctacctgcca ccgcgtcctg agtgaaatcg
     3061 acgccgaagt agcttcccca ggtcattccc atcgcgttga tgaaatagtt ccaggtcagg
     3121 aaactcacca tcaccgccag acaagcacgt ccctgcgctt gcttagcaag gccaatgggt
     3181 aaaccgacag caaaaatcag cggcatatta cggaataccg tccaaccgcc ctcttcaata
     3241 atgtgtacga tttgcgcgaa taaactgttc ggatcggtca gtgattcccc gacaaacatc
     3301 gggttttgca gcaagatggc aagacccacc acaatcccgg cgaagggaaa cagcagcact
     3361 ggcgtaaaca tcgcgccgcc aaagcgttga atttgactga gcatttccac atcctctttt
     3421 ctcaattctg taagtaggaa gcctgattgg ttatgcggca ggcctggagc aaggatagga
     3481 agatgcgatc ggcagaacat gacgacccaa tgggattcgt gatcgctttc atgcttttcg
     3541 ttgggcggta gagatctacg ttttcgcgaa aaagtagaca tctgtgagca caggtacaca
     3601 gcatcgaaca catctttaaa aaaaagatgt tttttcaatc gattaagcag aacttgtgtg
     3661 cgcattaccc gggcttgcag gcaaaaaaga gatctagaga tgatctacaa aagcattgcg
     3721 gagcggttaa gaattcgact taactccgca gatttcacgc taaacagcct tcttccgggt
     3781 gaaaaaaagc tggcggaaga gtttgcggta tcgcggatga ccatccgtaa agccattgac
     3841 ctgctggtag cgtgggggct ggtggtccgc cgccacggta gtggcactta cctggtgcgc
     3901 aaagatgtac tgcatcaaac cgccagtctg accgggctgg tggaggtgtt aaaacggcag
     3961 ggaaaaactg tcaccagcca ggtgctgatt tttgaaatca tgcctgcgcc tccggccatt
     4021 gccagccagt tacggattca aatcaacgag cagatctact tctcccgtcg cgttcgtttt
     4081 gtggaaggga aaccgctgat gctggaagac agctatatgc cggtaaaact gttccgtaat
     4141 ctttcgctgc aacatctgga agggtcgaag tttgaatata ttgaacaaga gtgcgggatt
     4201 ttgattggcg gtaattatga aagcctgacg ccggtgctcg ccgatagact gctggcgcgg
     4261 caaatgaagg tagcggaaca cacgccactg ctgcggatca cctcgttgtc atatagcgag
     4321 agcggggagt ttttgaatta ttcagtgatg ttcagaaatg ccagcgaata ccaggtggag
     4381 taccatttac ggcgactcca cccggaaaag agttaaccga tactccagaa gagcaccgcc
     4441 agtaattggg gggtgataat gcgcaggaac atcactaacg gatagacagt ggcgtaagag
     4501 agcgccgccg caccgctagt tggatgaaga ttattagcaa acgccagcgc cggaggatcg
     4561 gtcatggaac ctgccagcat cccgcacatg gtcaggtaat tcattttggc taacatccgc
     4621 gccagaatgc caacagtaat cagcggaacg gcggtgatca gggcaccata accaatccag
     4681 cttagccctt cgccattgac cagggtattc acaaaatccc caccagattt cagaccaacg
     4741 accgagagga acagcacgat ccccagctcc cgcagcgcga ggttggcact tggcggcata
     4801 aaccagtaca gcttgccgat actgccgata cgcccgagga tcaacgccat aatcagcaga
     4861 ccgcccgcca gccccagttt caacgcggcc gggaatcccg gcacaaagac gggaatagaa
     4921 cctaacaata cgccaagccc gatgccaata aacactggca gcatctgaac ctgttgcagt
     4981 ttttgctgcg cattccccag cacattggca acggcatcaa ttgcggacgg acgccccacc
     5041 aggttgagga tatcgccgaa ctgcaggctg atatcgccac tggcgaccag ttcgaccccg
     5101 gcacggttca ggcgcgagat aacaacgtca tagcgttctt taaagtgcag gtcgcgaata
     5161 cgttttccga gcacgttttc attggtgacc accacacgct cgacgcgcaa atcagtgtct
     5221 ttcgtggaca gcgaagtatc gacctcctga ccaatcacca gttgcgcatt atgtaaatcc
     5281 gctggctgac ccaccagatg cagcaaatcg cccagttgga taatggtatc tggcgaagga
     5341 acttttaggg tttcttcgcg tttcagacgc gagcagataa ttttgtcgcc gttgagaatc
     5401 ggtacatctt taatggctaa atcatgcagg ttagggttct caacgcgaat attgatagtc
     5461 ttgatcagcg cgccgccatt ggtgcgtgaa gactcgtgct gctgagcttc tgtctcgaca
     5521 ttgacgcgga aaataacccg caacatccac atggtgaaca aaatcccgca aatgccgaat
     5581 ggatacgcca tcgcgtaact catccccatc tgatcgacca tttccattgg tgtacccagg
     5641 tcgcgcaaaa tctgctgccc tgcccccagc gctggcgtat tggtaaccgc accggagaaa
     5701 atccccagca ctaccggcag tggaatatca aacagtttat gcaggatggc ggtaaccaga
     5761 ccaccgatga tgacgatcag aacagcaaac aggttgaggc gtaatccgga gacgcgcaat
     5821 gaggcaaaga agcccggccc tacctgaatc ccgatggtat aaacgaacag gatcaggcca
     5881 aattcctgaa taacatgcag catatcgcta cttaatgtca tccccgcctg agaaacaaaa
     5941 tggccgacga tgatcccacc aaacagcacg ccgccaatac ctaatcctat gccgcgaaat
     6001 ttgacgttgc cgataaacaa accgacgact gccaccaaag ccagaatact gaccgttaat
     6061 gctatatcac tcatcgtcca tttctcttga gataacattt taattgctac ggattatggc
     6121 tgaatcggtg aagcagattt gccttatagc gcacattatt acgggaaaga acgtgccgaa
     6181 atatcttaaa cagtcgaaat ttaacgcatt gttgaagaag aaagacaaaa aaagccccac
     6241 cagaatggcg gggcaaagag aatagctagt tagctattta acgcgggacg ttcgctgata
     6301 gcgatacgct gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa
     6361 ccgtttacga aagttgcgcc agagacttcc atattttcag ccagcgtaaa gctcaggcta
     6421 aatggctgat tcataagccc ttgatgcagc cattttttct cttcttttgg ctgctccggc
     6481 gtgcctttta cgctcaggcg cgtaccttcc agttgaatct ctaaatcttc ctgacggaaa
     6541 cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc
     6601 gggaagctct gactttcacc ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc
     6661 cattgacgca tcagtgggga taaatcgaag ttacgcatag tcatttctcc ttctaagaag
     6721 cgagtaagta cctgcaaatc cgaagattcg catatgctcc ctgacggcga gcatggagat
     6781 gtcaggccgc gccaggcggc cttaaggaat tagttgattt cgatacggcg cggttttttc
     6841 gcttccggaa tcacgcgttc gagatcgata tacagcaaac catttaccag gttagcgcca
     6901 cgaacatgaa tgttctcagc taactggaat ttgcgttcaa agttgcgttc agcgatgccc
     6961 tggtacagat aggtgcgctc tttttgttcg tcggcgtgag cacctttcac caccagcaga
     7021 ttatcctggg cggtaatttc cagttcgctc tcagcaaaac cagccacagc gatagcaatg
     7081 cggtaatggt tttcgtctac cagttcaacg ttatacggag ggtagccgcc attactctgg
     7141 ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc caatagcaga acggtaaagc
     7201 ggggataaat caaagttacg cataatcaat agctcctgaa atcagcgaga atgtaagacc
     7261 ttccacaatg gacaggtcag gtagccagaa cacccatcag gcgcgtcctc atcgactacg
     7321 atgtaaaaat gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag
     7381 tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgt gatagccgcc
     7441 acagagcgac gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca
     7501 acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat
     7561 atgcagcgga atggcattac tcggaggatg ctccagcgtg atgtcccata ccggagggaa
     7621 agaaggaacg tatcccggca cccgcgccag cgccaccatg attggcgatg atgaaaccaa
     7681 ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtga tggacacgct
     7741 tttattaccg tgggatgtgt tccgcaaaga cagctcggtg cgctcacgcg ttgaaaaaag
     7801 cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc ctgataactg
     7861 atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc cagccattga
     7921 ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa
     7981 tactcaacct cgccgctttg cgcatgggca caagcaattc gattatccag cacaaagccc
     8041 aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt gttatgggtt
     8101 aactggcgcg gctcgccgcc ctgtggcgag ataagccaca gttgcacaat gccgttatca
     8161 tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg
     8221 acgttgacta accccggata agcccgatgg tgggtaaagg ttaaacgtcg ctgcacgacg
     8281 ccacgcggtg gcgcgggcag ggtggtttcc gttccactta acggcgcatc acctgccgct
     8341 ttccagccag cttcatcttg cggcaactca acgataaaca gctccggcac tttctcaccc
     8401 tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca tccttcttca
     8461 taagcacgat tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt actcaccagc
     8521 acacaccagt ggctgccgct gtattcacgc ggatgctgct tttgtacgtt gaccggacca
     8581 aacggcgcag caacaccgac gtttcgcaaa tccagcgccg gatcgagttc atgcattaca
     8641 tggtcgttat aggtaaagct caccctttca ccgttcgggc taaagacatg cacatggctg
     8701 ccgccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc gagattgctc
     8761 accttgccgc cttcagcaat gactccgcgc cgatgatgga agtcgtaatg ccatgtttca
     8821 tcaggatttt ccgggccgtg aataaagaca tatttctctg acttcggatg aacggtcacc
     8881 acgccgacgt gtgcgccctg tgacgcacag tagataacct cgacctcacc ggtatggata
     8941 ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac gtcaaatacc
     9001 agccactggc tgtcgggcgt ccaggtattg gtattggtga gcaggtgatt acggggagca
     9061 aaggttatct gtttcatggc gcggtcctga tacaaaacgg gtccagccat catacttcac
     9121 aaggacttca ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg
     9181 gaacatttcg acgtggcgat tattggcctc ggcccggcag ggtcggcgtt ggcacgaaag
     9241 ttagccggca aaatgcaggt gatcgcgctg gataaaaagc accagcatgg tactgaaggt
     9301 ttcagcaaac cttgtggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat
     9361 gggctgacgc ttcctgtcga tgtgatcgct aatccgcaga ttttcagcgt caaaaccgtc
     9421 gacgtcgccg catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat
     9481 gctttcgact tgtggatgaa atcgctgatc cccgccagcg ttgaggttta tcacgacagc
     9541 ctgtgtcgaa aaatctggcg tgaggatgat aaatggcatg tcatttttcg tgcagacggc
     9601 tgggagcagc atattaccgc ccgctatctg gtcggtgccg atggtgccaa ctcgatggtg
     9661 cggcgacatc tctacccgga tcatcaaatc cgtaaatatg tcgcaatcca gcagtggttc
     9721 gcagagaaac atccggtgcc gttctactcc tgcatctttg ataatgcgat aactgactgt
     9781 tattcatgga gtatcagcaa agacggttat tttatctttg gcggtgccta tccaatgaaa
     9841 gacggtcaga cgcgtttcac gactctgaaa gagaaaatga gcgcctttca gttccagttt
     9901 ggcaaagcgg tgaaaagcga aaaatgcacg gtgctgtttc cctcacgctg gcaggatttt
     9961 gtctgcggta aggacaacgc ctttctgatt ggtgaagcgg cgggatttat cagcgccagc
    10021 tcgctggagg ggattagcta tgcgctggat agcgcagaca ttctgcgttc ggtgttactg
    10081 aagcagccag agaagctcaa taccgcttac tggcgcgcca cccgcaaact gcgtttaaaa
    10141 ctcttcggca agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt
    10201 atgcgcagtg gtgtggcaca tattccacag ttgaaggatt agccaacgcg cttcacatcg
    10261 cccaccagca ggatataaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt
    10321 gcaggtgcga aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg
    10381 cccagaccac cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc
    10441 agagaagaaa ccagcgacca ggtaattgaa gcaaaaccgt taccgaagaa tgccagcgcc
    10501 atcaggcaca taatcatcat cggatcgtta gtgtagttag cgcccataat gcaggtggag
    10561 atcagcaagc cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt
    10621 accagcagat ccgctaccca gccagagagc aggacgccga caaacgccgc gaggaatggc
    10681 accgtggtca taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc
    10741 gggaaccagg ttaagaaaaa ccacagtgta gaagccaccg caaattgccc aagatagacg
    10801 ccgatcagtt tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgtgcc
    10861 tctttcttca ctggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt
    10921 tcagctttgc tgatgccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc
    10981 gaccagataa tgccgatacc accagtcaca atgaacaccc agtgccagct caacatctcc
    11041 tgaatccaga tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa
    11101 aaaccaacgg cggaggcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg
    11161 gtcgggaaag cgggcgcttc gaaaataccg gttatcgcac gcagaccaat taatgacatt
    11221 aatcccgtgg caaagccctg gaataaagtc gccactgacc agccaaatat cgcaataaaa
    11281 taagtcacgc gagaacctac gcgatctaaa aaccaaccgc cggggatctg gcatagcgta
    11341 taaagccagg cgaaggccga aaatacatag cccatttccg ctttcgtaat gccgaactct
    11401 tcctgaatat gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc
    11461 gtaataaaga tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg
    11521 ggaatatcca tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc
    11581 tgtaatttac agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac
    11641 gtggcaggaa ttaccactct gctacgctgt tatcttcatg acgccagagt ggattacgcc
    11701 agtccggggc atttttactg aactcaataa ctttagcttc gtcgatttcc acgcctaagc
    11761 ccggtttcgt tagcggttta aagaagccgc caaccatgct gaagtcttct ttgtttttca
    11821 caaagtcgag taactccgcg cctttgttgt aatgaattcc catactttgt tcctgaagta
    11881 ccgcgttata ggaaacaaag tcgatatgca gacaagccgc cagtgcaatc ggtccgagcg
    11941 gacagtgcgg cgcaagggtc acgtcatagg cttctgccat tccggcaatt ttgtagcatt
    12001 cggtaatacc gcctgcatgg gagagatccg gttgtagaat cgaaataccg cctgcctcca
    12061 gcacgcgttt aaaatcgaaa cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg
    12121 tttgtgccgc caatttcggg tagtattcgg cttgttccgc cagcaccggc tcttcaataa
    12181 acagcgggcg atacggctcc agttctttaa tcagcacttt cgccattggc gcgctgacgc
    12241 gaccgtggaa atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca
    12301 cggtgttgac tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac
    12361 cgttcagttt gaaggtatcg aagccgattt cgcgcagggt tttaataccg tcgatcacat
    12421 ccgccggacg atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc
    12481 ccatcagttg ccagaccgac gcattcagca ctttaccttt gatatcccat aacgcctggt
    12541 cgatcccggc aatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca
    12601 tcacttgcca taagtcattg atgcgctatg gatcctgacc aatcaaatag tcacccagct
    12661 cgtgaactgc cgcttccacc gtgcgggcgc ggccttcaat cactggctcg ccccagccga
    12721 cgacgccttc atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg
    12781 taattttggt gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg
    12841 ctgcgcggtg cgttctacgg actgcccggc gcgatagaga tcgctgccta agcccgcccc
    12901 agcacaacct gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa
    12961 gactgcgatg tccgatggca ataccgcttt taacgctttg atgtattgcg gaccaaaagc
    13021 cgatgacgga aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt
    13081 cgccgtcgcg cagccggggc agacggtcat gccgtagccc accgcacggc ggatcacttc
    13141 actatggata ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg
    13201 ttcaggtttc agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat
    13261 ggcgggaatg ctttgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc
    13321 ggcgtcaatc accgcgccaa catgcgccag cgcctcgtcg ggcttaatgc cgcgcaaaat
    13381 ggcgatcagc ggaagtttag tttgccactg catgagcgat gctccttata ccagcctgaa
    13441 atgccgtgtc gcccgccacc gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt
    13501 agcgcgcggt caacgatgtt ccggcgacaa gggtgatggc gtgttgatgg gccacatagt
    13561 cgcgcatact ggcgacctct gcgccaatca acaaaccaga gagaaattca ctgacctgtt
    13621 cacggggaag tgttcccagc acatgcgagg cacgaacttc aaaaagctgc ggcaatatgg
    13681 cgggcgtatt aagaccacgc tcaaggccag ccgtgaaggc atcggcagag ttttcctgcg
    13741 gcggcaaacc tgcaccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca
    13801 tcacggtgcg aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac
    13861 cgggcatgac ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgagtttctt
    13921 cgccgcgcat cacattgtgg ttatcatcat gagagacgca taatccggga ataatccaga
    13981 tattgtcgcc aacagacgtt aattgttcgc caatagacga aaaatgggca ggaacagata
    14041 aatacggagc aactttccag ccgacattgc tgccgaccat tcctgccatt accactggcg
    14101 tattctcttc acgccagtcg gtcgtgactt ctgctaacac cgcagccgga gattttccgt
    14161 tcaggcgcgt gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa
    14221 gccaggcgcg cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat
    14281 ttcctttaac cttcgtgtcg agctggcgat catggtaagc gccgcctgct ctgccgcatc
    14341 gccgtcctga tgccgtatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat
    14401 gttggcctca tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc
    14461 aatgctaagt tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg
    14521 aatatccgct tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc
    14581 cgattcaatc tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc
    14641 cggttccacc agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt
    14701 ttccagcacc cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac
    14761 aaacgcgccg cgataacgtt tcatttcaat cagccgtttc gccatcaacg aacggaacac
    14821 ctcacggatg atgttgcgcg aggttgcaaa ctcctcacag agttccgcct cagccggaag
    14881 cggcgagcct ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacgatgcg
    14941 atcggtttta ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac
    15001 cccgatcgat gcataatcag gaaagtatca atcgcagtca gacaccatca cggggctatc
    15061 gcaggcaaag gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta
    15121 ccttatcaca gatggtgccg atgacatcgt taagatgctc aaggatccag ctcttaaccg
    15181 gcacaatatt caggttgctg atgacgcaag atttgtttta aatgcgggga aaaagaaatt
    15241 taccggcaca atatcactct attacaactg gagcacagaa gaagaaaaag cacgagtgac
    15301 acaatatcgt tttgcttgtg gtgttcagca ctgtacccgg acagtagaaa acctggaagg
    15361 tactatccat gagaaaaata aaaacatgga ttactcaaac gtaatggctt tctaccatcc
    15421 atttaaagtg cgattttatg aataccattc acccagaggc attccggatg gtgtttccgc
    15481 agcattactg ccagtgactg ttacgctgga catcattact gcaccgctgc aatttctggt
    15541 tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc
    15601 ccacagatta attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttag
    15661 atttggtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    15721 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    15781 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    15841 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca
    15901 cttcctgcgg aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca
    15961 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaatag
    16021 tggcaacgaa agattcatgc actgtgtagt agctgatatc gcggttggcg gtgtacagcg
    16081 tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    16141 gatagtcgtc atagctgaga gcagtttgcg ccacggtgct accatcagct gccttctgta
    16201 ctagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    16261 ggtagttgtg cacacctgca tacgggcgac ccgtcgttag cacgacattc acgccacggg
    16321 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa
    16381 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccca ggtagattgg
    16441 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    16501 gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc cctttcagca
    16561 gttccatacc tgcggaaagc agatcgttat tggcttgtgg tgacacttca ccttgcggtg
    16621 agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagct gtactggtat
    16681 ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gattccagtt
    16741 gctcgccact aaccgattga ttgccctgtt gattactcag ccaggttgag agaatggccc
    16801 ctaagccgcc actttgcagt ttttccagca gcacctgaat gccgccctgc tcctcaaccc
    16861 aacttaaaat agcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat
    16921 caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatt
    16981 tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt
    17041 cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgaaaa
    17101 ttcaatacat tgcaagattt tcgtaggcct gataagcgta gcgcatcagg cacgctcgca
    17161 tggttagcgc cattaaatat cgatattcgc cgctttcagg gcgttctctt caataaacgc
    17221 acggcgcggt tcaacggcgt cgcccatcag cgtggtgaac aactggtcgg cagcaatcgc
    17281 atctttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag
    17341 ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc cgcgacggga
    17401 ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac gacgctcgcc
    17461 acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat
    17521 acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag tatccacacc
    17581 gtgggtacgc acgcgaacaa tcggttcaaa caggttttgt tcggcattgg tgtggacatc
    17641 aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac
    17701 ccagcgggta acggtctgct catcagagag gtcggcttcc gtcagcgtcg gctgatagat
    17761 aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt
    17821 cgcgttgtac tcagacacca atttctctaa cgcttcgcca gccaatgccg gtgcgctggc
    17881 gttggtgtgc agcgttgcgc catccagcgc gatagagatc tggtactgat ccatcgcttc
    17941 gtcgtcttta atgtactgtt cttgcttgcc tttcttcact ttgtacagcg gcggctgagc
    18001 gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaaa aggtcaacag
    18061 cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata
    18121 acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg cggtgataag
    18181 cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcaa cgttgaggat
    18241 tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc
    18301 gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc gttcctggca
    18361 gtctgccagt ttgcccggca ggcccgctaa gtcgagcgca cctttacggc gggtcatttc
    18421 acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc caaccacgat
    18481 tttcgcgtcg gttgggtttt ccagcaggta ttctgccagc agttcgttca tctgctgttc
    18541 aaccgctgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg agaatttcgg
    18601 gtccggcact ttcacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc
    18661 gctaactttg gcttttttgc tgtagccttc tttgtccatg taggcgttca gggtacgggt
    18721 catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt
    18781 aaagcagtag atgttttcct ggaagccatc gttccactgc aacgccactt cgacgccaat
    18841 accgtctttt tcagtggaga agtagaagat attcgggtgg atcggcgttt tgttcttgtt
    18901 cagatattca acgaacgcct tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc
    18961 gcgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg
    19021 tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga ggctgggcca
    19081 gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccaacg gtgcctgcgg
    19141 tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctggataa ccagctccag
    19201 tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt
    19261 ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc
    19321 cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga
    19381 gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc
    19441 gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt
    19501 gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat
    19561 actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat
    19621 ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa
    19681 cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg
    19741 caagcagccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac
    19801 accgccgccc gctttcacgg gtgaggaact ctccttgcgc cagacgtaag gcgcacatca
    19861 acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc
    19921 gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat
    19981 tacgttccag cacctcagca tattctgtct ctttctccca gccgcgctgg aaagagaaag
    20041 tcagagaaaa ctcagggaga aattgcttac aagtatcggc catatcagcc gcgataccgg
    20101 cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagc tctttatccc
    20161 acgggcgtag ctgttcgtaa cgtgtcacct ggcgcagcgc cgcattgcgc tgcttgagca
    20221 atcgcttgag attgctccag gcggtgaaaa atccgggttc gttgtgaaag catccccagt
    20281 cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta
    20341 tcaactgcat tggcatcagg tgcgccagtt ccgcgacctt atgcccgtct gtaccgtcga
    20401 tgcggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgctgtc tcgcgctctt
    20461 cgccctgtaa tcgcccgtgg agaacaaacg cctcctgctc atggcgaatg acgcgaccaa
    20521 tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc agcacgctgg
    20581 ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa gcgagatccg
    20641 cggtttcaat gttgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat
    20701 tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca cgctggaaac
    20761 cgaatcggtc agcatcatgc ggacgttttc acatttcagc gcgttcagaa catccagcac
    20821 gtagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc
    20881 ttccgcttct tcctgttccg ggttgttggc ggtgattttc agctggtttt cgctgacata
    20941 aagacgtacg ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt
    21001 gagcagatcg cagccagctt ccagatgttt gtccgggttc ttcggcagaa cgcggcgata
    21061 atccgggaag cgaccatcca ccagtttgga ggtgaagata aagtcgccaa cgtgggcgcg
    21121 aatgttgttg ctgccaatct gtacgcgcag cggattgtcg ccgccgtcga gcatacgcat
    21181 cagttcaatc acgcctttac gcggcacgat caccgaatgg cttggcaaag attgaccaat
    21241 tggcattgaa cagactgcca gacggtggcc gtcggtcgcc acggtgcgca gttcttcgcc
    21301 ttcggtttca aacagcatac cgtttaagta atagcgaacg tcctgatgcg ccatagaaaa
    21361 ctgggtcgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct
    21421 ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg aaaaacggct
    21481 acgcccggag cgcaccagca tccgttcacc ttccagctgt acggcaattt ccgcgccttc
    21541 aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg ctcctggctc
    21601 gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag taccggtcag
    21661 cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg
    21721 accacctaac ggcccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt
    21781 aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct tctttgatat
    21841 cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt
    21901 cacgaccacc aaacgcatcg ccaatctccg gcagactgtg gttagtcagc tctttcgcca
    21961 gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat
    22021 ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca
    22081 gtttttcctg caatgccagc aagtcgcgca gtgcctcacg cacgaagtcg atggtgatcg
    22141 cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc agctcacgta
    22201 cgttagatcg tagacgcttg gcgataaaga acgccacttc gcctggcaaa cgaatgtcat
    22261 tttcgtcggc ctttttcatc aggatcgcca cgcgggtttc cagctctggc ggttcgatcg
    22321 ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt
    22381 tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg
    22441 tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca
    22501 gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg
    22561 ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct
    22621 tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc agacccgtgc
    22681 cgccataaag gaacaatggg ttataggcac cgccagggtt atccgccacc tggcgagccg
    22741 ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt
    22801 ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa cctgagcgcg
    22861 tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc
    22921 tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct
    22981 gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt
    23041 cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca
    23101 gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca
    23161 atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag
    23221 tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgct
    23281 tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg
    23341 atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt
    23401 cccgatttat ccacaggact ttccagacct cgctaagtgt aaacgatcct gccgcgaggc
    23461 gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat
    23521 cgatctaata aagatctagg acgatccttg cgctttaccc atcagcccgt ataatcctcc
    23581 acccggcgcg ccatgctggt tttcactggt gtgaggtcgt acattttccc tgcgaaaagg
    23641 tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggagtgtaca attattacaa
    23701 tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc
    23761 caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca
    23821 accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa
    23881 tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa
    23941 gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa
    24001 ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta
    24061 ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga
    24121 aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc
    24181 gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg
    24241 ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc
    24301 gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct
    24361 actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg
    24421 caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc
    24481 tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta
    24541 acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc
    24601 tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca
    24661 acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa
    24721 ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt
    24781 gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg
    24841 ctggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc
    24901 ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg
    24961 ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac
    25021 acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac
    25081 gtgcagaacg ctggcgagaa accgctggaa atctccacct ttggtcagtt gaagcaatcc
    25141 atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgc
    25201 ggcgcggcgt actccacgcc tgacgagaag tacgagaaat acaagttcga taccattgcc
    25261 gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcaatgct gcaacagtat
    25321 ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg
    25381 ggtaacggca tcgccgctat cggctataaa tctcagccgg tactggttca gcctggtcag
    25441 actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa aatggcagct
    25501 gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagccg
    25561 ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc
    25621 atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc
    25681 atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat
    25741 gacaaacagc gtatcagcca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg
    25801 ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg
    25861 ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg
    25921 gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag
    25981 aagatgtcgc cgaccacagt gaccgacccg atgcagcaga agatcatgac ctttatgccg
    26041 gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc
    26101 agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc
    26161 ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg
    26221 tcagttgacc gccttttttc ttttcgtagg gtggataagc accgcgcatc cgccacacaa
    26281 agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga
    26341 cgtggcggcg ttggcatcct gcgcatctcc ggcctaaaag cccgtgaagt tgccgaaacc
    26401 gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa agacgccgac
    26461 ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc
    26521 gaagatgtgc tggaactgca gggtcatggc ggtccggtga tcctcgacct gctgttaaaa
    26581 cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg
    26641 tttcttaacg ataaactcga cttagcccag gccgaggcga ttgccgatct tatcgacgcc
    26701 agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg
    26761 gtcaaccatc tggtggaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat
    26821 ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca gcttaatgac
    26881 gtgattgccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa
    26941 gggatgaagg tggtgattgc cggacgtcct aacgccggta aatcgagcct gttaaacgcg
    27001 ctggcgggcc gtgaagcagc aatcgtaact gatatcgccg gaaccacacg tgacgtgctg
    27061 cgtgagcata tccacattga cggaatgccg ctgcatatca tcgataccgc tgggctacgt
    27121 gaagccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga aattgaacag
    27181 gccgaccgcg tgctgtttat ggtcgatggc accacaacag acgccgtgga tccggcagag
    27241 atctggccgg aatttatcgc ccgtctgcca gcgaaactac cgatcaccgt ggtgcgcaat
    27301 aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca cgcgttaatt
    27361 cgtctctcgg caaggacagg tgaaggcgtg gaggtgctgc gtaaccatct caaacagagc
    27421 atgggctttg acaccaacat ggaaggcggc ttcctggcgc gtcgtcgcca cctacaggcg
    27481 ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttagg tgcctgggcg
    27541 ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga aatcaccggg
    27601 gaatttacct ctgatgattt gctggggcgg attttctcca gcttctgtat tggtaagtaa
    27661 ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt gttagcactc
    27721 ctgttattcc tcaacccttt ttttaaacat taaaattctt acgtaattta taatctttaa
    27781 aaaaagcatt taatattgct ccc
//
LOCUS       DAEEBB010000045        27503 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEEBB010000045 DAEEBB010000000
VERSION     DAEEBB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.045"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(36..119)
                     /locus_tag="KLF07_001799"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="KLF07_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="KLF07_001800"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="KLF07_001800"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBD9831827.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="KLF07_001801"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="KLF07_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBD9831828.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="KLF07_001802"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="KLF07_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBD9831829.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="KLF07_001803"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="KLF07_001803"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBD9831830.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="KLF07_001804"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="KLF07_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBD9831831.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="KLF07_001805"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="KLF07_001805"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBD9831832.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="KLF07_001806"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="KLF07_001806"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBD9831833.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="KLF07_001807"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="KLF07_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="KLF07_001808"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="KLF07_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBD9831834.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="KLF07_001809"
     CDS             complement(12425..12664)
                     /locus_tag="KLF07_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831835.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="KLF07_001810"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="KLF07_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBD9831836.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="KLF07_001811"
     CDS             13041..13328
                     /locus_tag="KLF07_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBD9831837.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13998..14141
                     /locus_tag="KLF07_001812"
     CDS             13998..14141
                     /locus_tag="KLF07_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBD9831838.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14154..14363
                     /gene="cspE"
                     /locus_tag="KLF07_001813"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14154..14363
                     /gene="cspE"
                     /locus_tag="KLF07_001813"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD9831839.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14529..15338
                     /gene="rlmA"
                     /locus_tag="KLF07_001814"
     CDS             14529..15338
                     /gene="rlmA"
                     /locus_tag="KLF07_001814"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBD9831840.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="KLF07_001815"
     CDS             complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="KLF07_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBD9831841.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16330..16788)
                     /locus_tag="KLF07_001816"
     CDS             complement(16330..16788)
                     /locus_tag="KLF07_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBD9831842.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="KLF07_001817"
     CDS             complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="KLF07_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBD9831843.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17707..18507)
                     /gene="manY"
                     /locus_tag="KLF07_001818"
     CDS             complement(17707..18507)
                     /gene="manY"
                     /locus_tag="KLF07_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBD9831844.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18570..19541)
                     /gene="manX"
                     /locus_tag="KLF07_001819"
     CDS             complement(18570..19541)
                     /gene="manX"
                     /locus_tag="KLF07_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBD9831845.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20004..21554
                     /gene="yoaE"
                     /locus_tag="KLF07_001820"
     CDS             20004..21554
                     /gene="yoaE"
                     /locus_tag="KLF07_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBD9831846.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21558..23156)
                     /locus_tag="KLF07_001821"
     CDS             complement(21558..23156)
                     /locus_tag="KLF07_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD9831847.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="KLF07_001822"
     CDS             complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="KLF07_001822"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD9831848.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24835..25413)
                     /locus_tag="KLF07_001823"
     CDS             complement(24835..25413)
                     /locus_tag="KLF07_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBD9831849.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="KLF07_001824"
     CDS             complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="KLF07_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBD9831850.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26852..27031
                     /locus_tag="KLF07_001825"
     CDS             26852..27031
                     /locus_tag="KLF07_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBD9831851.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27151..27474)
                     /locus_tag="KLF07_001826"
     CDS             complement(27151..27474)
                     /locus_tag="KLF07_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBD9831852.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7081 a         6774 c         6943 g         6705 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    13501 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    13561 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    13621 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    13681 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    13741 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    13801 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    13861 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    13921 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    13981 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    14041 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    14101 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    14161 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    14221 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    14281 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    14341 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    14401 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    14461 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    14521 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    14581 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    14641 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    14701 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    14761 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    14821 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    14881 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    14941 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    15001 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    15061 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    15121 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    15181 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    15241 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    15301 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    15361 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttctgctttt ttcccaataa
    15421 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
    15481 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
    15541 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
    15601 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    15661 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
    15721 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    15781 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
    15841 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    15901 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    15961 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    16021 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    16081 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    16141 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    16201 gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    16261 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    16321 attcataact tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt
    16381 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
    16441 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
    16501 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
    16561 tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag
    16621 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
    16681 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
    16741 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
    16801 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
    16861 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
    16921 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
    16981 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
    17041 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
    17101 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
    17161 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
    17221 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
    17281 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
    17341 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
    17401 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
    17461 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
    17521 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
    17581 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
    17641 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
    17701 cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc
    17761 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat
    17821 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
    17881 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
    17941 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
    18001 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
    18061 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
    18121 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
    18181 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
    18241 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
    18301 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
    18361 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
    18421 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
    18481 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
    18541 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
    18601 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
    18661 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
    18721 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
    18781 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
    18841 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
    18901 ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta
    18961 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
    19021 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
    19081 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
    19141 accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc
    19201 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
    19261 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
    19321 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
    19381 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
    19441 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
    19501 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
    19561 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
    19621 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
    19681 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
    19741 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
    19801 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
    19861 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
    19921 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
    19981 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
    20041 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
    20101 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
    20161 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
    20221 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
    20281 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
    20341 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
    20401 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
    20461 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
    20521 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
    20581 tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
    20641 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
    20701 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
    20761 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc
    20821 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
    20881 ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg
    20941 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
    21001 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
    21061 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
    21121 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
    21181 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
    21241 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
    21301 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
    21361 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
    21421 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
    21481 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
    21541 atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg
    21601 gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt
    21661 gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct
    21721 tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg
    21781 atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca
    21841 attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg
    21901 aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca
    21961 acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg
    22021 ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg
    22081 acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt
    22141 tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata
    22201 aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca
    22261 ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt
    22321 tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag
    22381 gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag
    22441 tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc
    22501 gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta
    22561 ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt
    22621 ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg
    22681 ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg
    22741 tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg
    22801 ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca
    22861 atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag
    22921 cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact
    22981 ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta
    23041 cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta
    23101 caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg
    23161 gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa
    23221 tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa
    23281 gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt
    23341 ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg
    23401 cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat
    23461 ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc
    23521 aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag
    23581 cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc
    23641 aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc
    23701 cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc
    23761 cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc
    23821 ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat
    23881 cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg
    23941 tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc
    24001 ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc
    24061 cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag
    24121 cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc
    24181 aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac
    24241 gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc
    24301 gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag
    24361 cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac
    24421 ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt
    24481 acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa
    24541 gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg
    24601 ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact
    24661 cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga
    24721 acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact
    24781 aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg
    24841 tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa
    24901 atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa
    24961 agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac
    25021 ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac
    25081 ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg
    25141 cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt
    25201 gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga
    25261 acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc
    25321 ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa
    25381 aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat
    25441 cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat
    25501 tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat
    25561 gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg
    25621 tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat
    25681 tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg
    25741 tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc
    25801 tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac
    25861 aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct
    25921 atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc
    25981 actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag
    26041 acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca
    26101 ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc
    26161 gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt
    26221 ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag
    26281 ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg
    26341 gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg
    26401 ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag
    26461 tgcgccgccc tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag
    26521 cacctgctgg agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact
    26581 aacaacggtt tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc
    26641 aaagcggcta tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg
    26701 gcttaagcgg gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc
    26761 gggagataac gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata
    26821 attagcgcca acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca
    26881 tgaacagcag caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag
    26941 cggccaggca atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat
    27001 cgtggcgcgt tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac
    27061 aagccgatga ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc
    27121 ctgatccatt tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat
    27181 atatgttgaa aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt
    27241 tgcccatcca accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc
    27301 cgtaatatca gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt
    27361 cgccgtgctt tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa
    27421 atccagggct ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc
    27481 gataaggcta tatatgacct taa
//
LOCUS       DAEEBB010000046        27105 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEEBB010000046 DAEEBB010000000
VERSION     DAEEBB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.046"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..924
                     /locus_tag="KLF07_001827"
     CDS             <1..924
                     /locus_tag="KLF07_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831853.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="KLF07_001828"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="KLF07_001828"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBD9831854.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="KLF07_001829"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="KLF07_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBD9831855.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="KLF07_001830"
     CDS             3885..4280
                     /locus_tag="KLF07_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBD9831856.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="KLF07_001831"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="KLF07_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBD9831857.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="KLF07_001832"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="KLF07_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBD9831858.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="KLF07_001833"
     CDS             complement(5589..6629)
                     /locus_tag="KLF07_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD9831859.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="KLF07_001834"
     CDS             complement(6702..7337)
                     /locus_tag="KLF07_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBD9831860.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="KLF07_001835"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="KLF07_001835"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBD9831861.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="KLF07_001836"
     CDS             complement(7963..8406)
                     /locus_tag="KLF07_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBD9831862.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="KLF07_001837"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="KLF07_001837"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBD9831863.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="KLF07_001838"
     CDS             complement(8746..9249)
                     /locus_tag="KLF07_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD9831864.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="KLF07_001839"
     CDS             complement(9243..9767)
                     /locus_tag="KLF07_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBD9831865.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="KLF07_001840"
     CDS             9976..10971
                     /locus_tag="KLF07_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD9831866.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="KLF07_001841"
     CDS             10980..11858
                     /locus_tag="KLF07_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD9831867.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="KLF07_001842"
     CDS             12064..13071
                     /locus_tag="KLF07_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBD9831868.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="KLF07_001843"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="KLF07_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBD9831869.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="KLF07_001844"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="KLF07_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBD9831870.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="KLF07_001845"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="KLF07_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBD9831871.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="KLF07_001846"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="KLF07_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBD9831872.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="KLF07_001847"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="KLF07_001847"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBD9831873.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="KLF07_001848"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="KLF07_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBD9831874.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="KLF07_001849"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="KLF07_001849"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBD9831875.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="KLF07_001850"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="KLF07_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBD9831876.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="KLF07_001851"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="KLF07_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBD9831877.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="KLF07_001852"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="KLF07_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBD9831878.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="KLF07_001853"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="KLF07_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBD9831879.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="KLF07_001854"
     tRNA            complement(26829..26905)
                     /locus_tag="KLF07_001854"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6420 a         7518 c         6811 g         6356 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aagtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc cccct
//
LOCUS       DAEEBB010000047        26836 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEEBB010000047 DAEEBB010000000
VERSION     DAEEBB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.047"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            202..474
                     /locus_tag="KLF07_001855"
     CDS             202..474
                     /locus_tag="KLF07_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBD9831880.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="KLF07_001856"
     CDS             567..650
                     /locus_tag="KLF07_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831881.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="KLF07_001857"
     CDS             750..1571
                     /locus_tag="KLF07_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBD9831882.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="KLF07_001858"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="KLF07_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBD9831883.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="KLF07_001859"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="KLF07_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBD9831884.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="KLF07_001860"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="KLF07_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBD9831885.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="KLF07_001861"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="KLF07_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBD9831886.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="KLF07_001862"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="KLF07_001862"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBD9831887.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /locus_tag="KLF07_001863"
     CDS             7217..8161
                     /locus_tag="KLF07_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD9831888.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="KLF07_001864"
     CDS             8347..9729
                     /locus_tag="KLF07_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD9831889.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="KLF07_001865"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="KLF07_001865"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBD9831890.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="KLF07_001866"
     CDS             complement(10485..10820)
                     /locus_tag="KLF07_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBD9831891.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="KLF07_001867"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="KLF07_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBD9831892.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="KLF07_001868"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="KLF07_001868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBD9831893.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="KLF07_001869"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="KLF07_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBD9831894.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="KLF07_001870"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="KLF07_001870"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBD9831895.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="KLF07_001871"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="KLF07_001871"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD9831896.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="KLF07_001872"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="KLF07_001872"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBD9831897.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="KLF07_001873"
     CDS             18642..20150
                     /locus_tag="KLF07_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD9831898.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="KLF07_001874"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="KLF07_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBD9831899.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="KLF07_001875"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="KLF07_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBD9831900.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="KLF07_001876"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="KLF07_001876"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBD9831901.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="KLF07_001877"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="KLF07_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBD9831902.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="KLF07_001878"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="KLF07_001878"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBD9831903.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6736 a         6948 c         6452 g         6700 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aaacccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcattt tccgtc
//
LOCUS       DAEEBB010000048        26570 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEEBB010000048 DAEEBB010000000
VERSION     DAEEBB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.048"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..296
                     /locus_tag="KLF07_001879"
     CDS             <1..296
                     /locus_tag="KLF07_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HBD9831904.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="KLF07_001880"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="KLF07_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBD9831905.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="KLF07_001881"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="KLF07_001881"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBD9831906.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="KLF07_001882"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="KLF07_001882"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBD9831907.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="KLF07_001883"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="KLF07_001883"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBD9831908.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="KLF07_001884"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="KLF07_001884"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBD9831909.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="KLF07_001885"
     CDS             7366..8796
                     /locus_tag="KLF07_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBD9831910.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="KLF07_001886"
     CDS             8895..9908
                     /locus_tag="KLF07_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD9831911.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="KLF07_001887"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="KLF07_001887"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBD9831912.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="KLF07_001888"
     CDS             complement(10732..11055)
                     /locus_tag="KLF07_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBD9831913.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..13035)
                     /locus_tag="KLF07_001889"
     CDS             complement(11266..13035)
                     /locus_tag="KLF07_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD9831914.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13684..14199
                     /locus_tag="KLF07_001890"
     CDS             13684..14199
                     /locus_tag="KLF07_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD9831915.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14417..15145
                     /gene="artP"
                     /locus_tag="KLF07_001891"
     CDS             14417..15145
                     /gene="artP"
                     /locus_tag="KLF07_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBD9831916.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            15163..15894
                     /gene="artJ"
                     /locus_tag="KLF07_001892"
     CDS             15163..15894
                     /gene="artJ"
                     /locus_tag="KLF07_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9831917.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15901..16617
                     /gene="artQ"
                     /locus_tag="KLF07_001893"
     CDS             15901..16617
                     /gene="artQ"
                     /locus_tag="KLF07_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBD9831918.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16617..17285
                     /gene="artM"
                     /locus_tag="KLF07_001894"
     CDS             16617..17285
                     /gene="artM"
                     /locus_tag="KLF07_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBD9831919.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17577..18308
                     /gene="artJ"
                     /locus_tag="KLF07_001895"
     CDS             17577..18308
                     /gene="artJ"
                     /locus_tag="KLF07_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBD9831920.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="KLF07_001896"
     CDS             complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="KLF07_001896"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBD9831921.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19651..20139)
                     /locus_tag="KLF07_001897"
     CDS             complement(19651..20139)
                     /locus_tag="KLF07_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBD9831922.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20199..21044)
                     /gene="potI"
                     /locus_tag="KLF07_001898"
     CDS             complement(20199..21044)
                     /gene="potI"
                     /locus_tag="KLF07_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBD9831923.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(21041..21994)
                     /gene="potH"
                     /locus_tag="KLF07_001899"
     CDS             complement(21041..21994)
                     /gene="potH"
                     /locus_tag="KLF07_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBD9831924.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(22004..23137)
                     /gene="potG"
                     /locus_tag="KLF07_001900"
     CDS             complement(22004..23137)
                     /gene="potG"
                     /locus_tag="KLF07_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBD9831925.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23232..24344)
                     /gene="potF"
                     /locus_tag="KLF07_001901"
     CDS             complement(23232..24344)
                     /gene="potF"
                     /locus_tag="KLF07_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBD9831926.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24695..25171)
                     /locus_tag="KLF07_001902"
     CDS             complement(24695..25171)
                     /locus_tag="KLF07_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBD9831927.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="KLF07_001903"
     CDS             complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="KLF07_001903"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBD9831928.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26222..>26570)
                     /gene="nfsA"
                     /locus_tag="KLF07_001904"
     CDS             complement(26222..>26570)
                     /gene="nfsA"
                     /locus_tag="KLF07_001904"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBD9831929.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         6555 a         6922 c         6570 g         6523 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt cactccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tctaaacgaa acatttttga atacattatt
    11821 tattatattg ctatttgtta ttttaacatc acctaaactg ccgttttcaa aacaacattt
    11881 tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta tgttttgctt
    11941 tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat tgagtagtgt
    12001 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
    12061 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
    12121 atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag gaaacagatt
    12181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
    12241 tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat ctaacgcagt
    12301 ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
    12361 gtcataaaaa gtgttcatca atgcgatatt gtttgttaca gcgtcttttt cgatattctc
    12421 actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct ttataaagga
    12481 tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa tcacattcgc
    12541 aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
    12601 gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact gttgtctttc
    12661 aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac ccgcctttat
    12721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
    12781 actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga gcaacacatc
    12841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
    12901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
    12961 atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg cttgatcaga
    13021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctcgt
    13081 gctttataat tataaaagca caacgccttt tatattcata ttaattaacg agatactgaa
    13141 acaacttaat ttattcttcg ctaaaattca ttagcttaaa ttctttagcc acaattgtag
    13201 cataaataat attatcgaga gtcaaagctg ggactatcaa atttcgctca ttttcaaaaa
    13261 tacaacgtaa ttttggcaat catcttaatc gcaggatctc ttatcgcatg tgaaaaattt
    13321 tgtaatgatt agacaataac cgcgttcatt tatcacgcgt tataaatgtg aaactggctt
    13381 cttgcagatg ctcttaacct ctacaggagg atttatacca ccatttcaga actatcatat
    13441 ttaccttaaa tgatcatttc agccatataa cacgctcaac ctcacaaatg gtttacactg
    13501 tctattgttt gggtaattta agtaagtcag ctacgagcaa ccttgagtgg atagccagca
    13561 atggactcaa acccaatcag gtgttcagat tgtttcagaa ttgtattaaa aacaatgtat
    13621 taaataatct gatagcgttt ggtcgtagtg tgacgcagac agcaagctaa taaggaaatg
    13681 attatgcgct accgttcact atctctactt attccatgtg cactgctgct gagtgcctgt
    13741 accaccgtca cgccagctta taaagataac ggtccccgca ccggttcctg tgtccagggg
    13801 gggcctgaca gcgtggctca acaattctat gactaccgca ttcaacatcg cagcaatgac
    13861 attaccgccc tgcgccccta tttgagtgac aaactggcaa cccttcttag cgatgccagc
    13921 cgcgataata gtcatcgtga attgttaagc agcgatccgt tctccagtcg aaccaccttg
    13981 ccagatagcg cccacgttgc cagtgcatcg actatcccta atcgtgatgc ccgtaacatt
    14041 ccattgcgcg tagatctcaa acagggtgac cagggttggc aggatgaagt gttaatgatt
    14101 caggaaggcc agtgttgggt catcgacgat gtgcgctact taggcggcag cgttcatgcc
    14161 acggcgggta cgctccgtca gtccatcgag aatcgctaac gccttgatca accaataaac
    14221 cccgtaaatt gtccggcatt tcgtgaggaa tgccgacatt tatgctcgcc gaccaccgcc
    14281 cccgttattt tgtgctatgt ttattgaata atgcgctttg cttttaactt ttaaagcaga
    14341 aatattgcat aattattctg tcaaaggtac tatcttcgac ctcaatagct atcagactgc
    14401 cagtatacga gtgtcaatga gtattcaatt aaacggcatt aattgcttct acggcgcgca
    14461 tcaggcgctg ttcgatatca cgctggattg cccacagggc gaaacgctgg tgttacttgg
    14521 ccccagcggc gcgggtaaaa gctcgctgct gcgtgtactc aatctgcttg agatgccacg
    14581 ctccggtacg ctcaacattg caggcaacca tttcgatttc accaaaacac cctctgacaa
    14641 agcgattcgc gatttgcgtc gtaacgttgg catggtgttt cagcaataca acctgtggcc
    14701 gcatctgacc gtgcagcaaa acctgattga agcgccctgc cgtgtactgg ggttgagtaa
    14761 agatcaggcg ctggcccgtg cagaaaaact gctggaacgt ctgcgtctca aaccttatag
    14821 cgatcgttac ccgctgcatc tttctggtgg tcagcagcag cgtgttgcta ttgcccgtgc
    14881 gttgatgatg gaaccgcagg tactgctgtt cgatgaaccg accgccgcac tggacccgga
    14941 aattacggca caaatcgtca gcatcattcg tgagctggca gaaacgaata ttacccaggt
    15001 gatcgtcacc cacgaagttg aagtggcgcg taaaaccgcc agccgagtgg tgtatatgga
    15061 aaatggtcat atcgtagaac aaggcgacgc gagctgcttt accgagccgc aaaccgaagc
    15121 atttaaaaac tatctctctc actaagattc gggataacaa caatgaaaaa agttctgatt
    15181 gccgcgttaa ttgcaggttt tagtctttcc gccacagctg ccgaaaccat tcgttttgct
    15241 accgaagcat cctatcctcc gtttgaatcg attgatgcaa acaaccagat cgttggtttt
    15301 gacgtcgacc tggcacaagc gctgtgtaaa gagattgatg caacctgcac tttctctaac
    15361 caggcgtttg acagcctgat cccaagcctg aaattccgtc gcgtagaagc cgtgatggcg
    15421 ggcatggata tcactccgga gcgtgaaaag caggtgctgt ttaccacccc gtactatgac
    15481 aactctgccc tgtttgtggg tcagcaaggc aaatacacca gtgttgatca gctgaaaggc
    15541 aaaaaagtcg gcgtacagaa cgggacgaca caccagaaat tcattatgga taagcacccg
    15601 gaaatcacta ccgttccgta tgacagctac cagaacgcaa aactggatct gcaaaacggg
    15661 cgtatcgacg gcgtcttcgg tgacaccgca gtggtcactg agtggctgaa agataacccg
    15721 aaactggcgg cggtgggcga caaagtgacc gataaagatt acttcggcac tggcctcggc
    15781 atcgcggtac gtcagggcaa cactgagctg cagcagaaac tcaacactgc gctggaaaaa
    15841 gtgaagaaag atggcactta cgaaaccatc tacaacaaat ggttccagaa gtaattcctg
    15901 atgaatgaat tttttccttt agcaagcgcc gccgggatga ccgtcggcct tgccgtttgt
    15961 gcattgattg tcgggctggc gctggcgatg ttctttgcgg tatgggagtc ggcaaaatgg
    16021 cgtcctgtcg cgtgggcagg ttcagcgctg gtaaccattc tgcgtggcct gccagaaatt
    16081 ctggtggtgc tgtttatcta ttttggctcc tcgcagctgc tgctgacgct ttcggatggc
    16141 ttcactatca atcttgggtt cgtgcagatc ccagtgcaga tggacattga gaacttcgac
    16201 gttagcccgt tcctttgtgg tgtcatcgct ctgtcactgc tgtatgccgc ctatgcctcg
    16261 caaacgcttc ggggcgcgtt gaaagcggtg ccggtgggtc agtgggagtc tggtcaggcg
    16321 ctggggctgt cgaaatcggc tatctttttc cgtctggtga tgccgcagat gtggcgtcat
    16381 gcgctgcctg gcctcggtaa ccagtggctg gtgctgctga aagataccgc gctggtcagt
    16441 ttgattagtg tgaatgattt aatgctgcaa acaaaaagca tcgctactcg tacccaggaa
    16501 ccatttacct ggtacattgt ggcggcggcg atttacctgg tgatcaccct gctcagtcag
    16561 tacattctca aacgcattga cctgcgcgcg acacgttttg agcggaggcc cagctaatgt
    16621 ttgagtattt acccgaactg atgaaagggc tacacaccag cctgacgcta accgttgcct
    16681 cgctgattgt ggcactgatt ctggcattga tttttaccat catcctgacg ctgaaaacgc
    16741 cggtgctggt gtggctggtg cggggttata tcacgctgtt taccggtacg ccgctgctgg
    16801 tgcagatctt cctgatttat tacgggccgg gccagtttcc gacgttgcag gagtatccgg
    16861 cactgtggca tttgttgtca gaaccgtggt tatgtgcgct gattgcgtta tcgctgaaca
    16921 gtgcggcgta taccacgcag ctgttttacg gtgcaattcg tgcgatccca gaaggtcagt
    16981 ggcagtcctg tagcgccctg ggaatgagca aaaaagatac gctggcgctc ctgctgccgt
    17041 atgcctttaa acgctcgctc tcttcttatt ccaacgaagt ggtgctggtg ttcaaaagta
    17101 cctctctggc ttacaccatt acgctgatgg aagtgatggg atacagccag ttgttgtacg
    17161 gacgcaccta cgatgtaatg gtgttcggtg cggcagggat tatttacctg gtcgtcaacg
    17221 gcctgctgac gctgatgatg cgtctgatcg agcgcaaagc actggcattc gaacggcgga
    17281 attaatgtag ctgcgtacat tcgccggggg ggtttgtagg tctgataaga cgcgccagcg
    17341 tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc gtggacgcct tatccggcct
    17401 acatagtgca taaattctta taatgaagac ggacaactca ctaagttatc cgtctttttt
    17461 tatttcattt aaattattta atcatattta ttgcatataa attcacttga tggcattgtt
    17521 atcccatgcc gcagacacgg ccaaaaatca taagattgac agacgggagt tccatcatga
    17581 aaaagttagt tcttgccgct ttacttgctt cctttacttt cggtgcttct gccgcagaga
    17641 aaatcaattt tggcgtttca gccacctatc caccctttga atctataggt gctaataatg
    17701 agattgtcgg ctttgatatc gatctggcaa aagccttgtg caaacaaatg caggcagaat
    17761 gtacttttac taatcacgcg ttcgacagcc tgatcccgtc actgaaattc agaaaatatg
    17821 actccgtaat ctccggtatg gatatcacgc ccgagcgtag caagcaggta tcgtttacca
    17881 cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg
    17941 ccgatctgaa aggcaaacgt attgggatgg aaaacggcac cacgcaccag aaatatattc
    18001 aggatcagca ccccgaagtg aaaactgtct cctatgacag ttatcagaat gcctttatcg
    18061 atctgaaaaa tggtcgtatt gatggggtat ttggtgacac agcggtggta aacgaatggc
    18121 tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg
    18181 gcaccggtct gggcatcgct gtacgtccgg ataacaaagc tctgctggaa aaactgaata
    18241 acgcgctggc agcaattaaa gctgacggta catatcagaa aatcaatgac cagtggttcc
    18301 cgcagtaatt gagactgatg acaaaagtaa gattgcctga tgcgctgcgc ttatctggcc
    18361 cacatcaact ctgcaatgta ttgatttagc atgattttgt aggccggata aggcgtacac
    18421 gccgcatccg gcatgaacaa agcgcacctg atcagcaatc tgaagccgca cctgcggctt
    18481 ttttattgct tcaccagcag cgtcatcact tcatagtgcg cggtgtgcgg gaacatatca
    18541 aaaagctgta cccgttcgat gcggtaacct ggcagttcgc ggatatcttt cgccatggtt
    18601 tgggcgttac agctggagta gataataaaa cgcggtgcca tcgttgagag ataatcacac
    18661 agcggtttac caatgccgcg gcgcggcggg ttaaccagca ccagctccgg cacatccccc
    18721 tgagcggtgg caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt
    18781 tcagcggctg actgctttgc acaggcaatg gcctctgatg caatttcgat cccggttaac
    18841 tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa cagatcccac
    18901 atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg tagcgtacag ctggctggcg
    18961 accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa
    19021 cgctctgcca gcgcctgttg ttcagtcagg tagatctccg tctccccttc cataatcgcc
    19081 atatgtaccg gctgaatatt gacggtaata actttcagtt gcggtagttg ttcctgtaac
    19141 cacggcagcg ccttacgcag ttgtgccagt ttggtttcag aacgcagtac aaagcgcagc
    19201 atcatgccgc catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta
    19261 cgcgccacgt tgtagggcgt taaccccgca cgggcgataa acggttttag cgccgcaaaa
    19321 acgggcgcaa aagaggcagg ataaagcggg cagtcacaaa ggtcttccgg cgtgccatcg
    19381 cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta
    19441 ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaaatcg
    19501 gcgagcagat ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt
    19561 atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct
    19621 tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt
    19681 cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct
    19741 atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat aaccgtaccc
    19801 cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt gtcagcagat atagccagcg
    19861 cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac
    19921 cagactgctt aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc
    19981 gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat
    20041 aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga
    20101 agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcggcatgc
    20161 gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccatgtct tgcacgctgg
    20221 atatcgcgta tccgctgttt ttctgcgcga gccatcagat accaggcgat aaatccgaca
    20281 attccgaccg caccgagaat taacgttgca agggcgttga tttccggatt cactcccatc
    20341 cgcacgctgg aaaagaccag catcggtaac gtggtggctc ccggcccgga aacaaagctg
    20401 gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg
    20461 ggcatgatca tcggtagcgt aatgacgaaa aacactttca gcggcgtcgc accgagatcc
    20521 atcgctgctt cttctatcga gcgatccagt tcccgcaatc ttgaagaaat aacgaccgcc
    20581 acataagccg tacaaaaagt gacatgcgcc agccagatgg tgagcatacc gcggtccgca
    20641 ggccagccaa tggcatgagc aagcgcgacg aataataaca acagcgacaa gcccgtgatg
    20701 ccatctggca tcaccagcgg cgcggtgatc ataaaggcaa aaccatttga tccgcgaaac
    20761 ctgccaaaac gcaccagcac caccgccgca atagtcccga ggatcgccgc cgccgttgcc
    20821 gcacaggcag caattgtcag gcttaaacca accgcactca tcatcgcgtc atcgcgcaat
    20881 aactcaccat accagcgcgt tgaccagccg gcccacaccg tcaccagctt cgagctgtta
    20941 aacgaataga taaccagcat cagcattggc gcgtagagaa aggtaaagcc cagcagcaaa
    21001 atcacaatcc gccagggcga acgaactacc ggtaaattat tcatccgtgt tctcccacgc
    21061 ttttttgctg gtgtttgtgg aaccacatta tcggcacaat tagcagcagc aacatgatga
    21121 tcgctaccgc cgaggccacc ggccagtcgc ggttattgaa aaactcttgc cataacacgc
    21181 gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac tcgcccacag
    21241 ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc ggcacgatca
    21301 cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag cgctgcttcc accagcgaat
    21361 aatcaatacg aatcaacgcg gtataaatcg gcagtaccat aaacggcacg taagcgtaaa
    21421 caatgccgat ataaacggcc agattggtat gcagaatggt cagcggttga tcgataaccc
    21481 ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc atccaggcat
    21541 aaacgcggat cagaaacgag gtccacgacg gcaggatcac cagtagtaat aaaatattac
    21601 gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc agtaaacagc
    21661 aaaatgtcga aatcgccgcc acctgtaacg actggagata agcatcaaaa tagagcggat
    21721 cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat ggaaagttgc ccgtcagccc
    21781 actccatcag ttcggtataa ggtggaatag cgcgcgccat ctctcccagg cttattttaa
    21841 agacaatcag aaatggcagc agaaacagca agatcaacca gatatatagc aacgcaatga
    21901 ccagtttgcg cccatgcttc atttgcagct gcgacaacca cagcttaaat ccgcccggcg
    21961 gtttcgactg ggcagcaggt tcaagtgtac tcatctttgc tccttaaacc gtcagcacca
    22021 cacagctgtc cacttcccag cacaaacgca cttcgtcgcc ccaggtcggt aaccctttac
    22081 gatgacgatg ggcgttttgt agctgggcgc tgatcatctg cccacttttc agtcgaacgt
    22141 gatacaccga aagatcgccg agataggcaa tgtgtatcac ctcccccacc gcgaagttac
    22201 aaccattggc gggcggctct tcgcaaagca tgattttttc cgggcgcagc gctacgtgta
    22261 ccggcacgtt atcgaccact gaagcatccg catcgacttt cagtggatgc accagccccg
    22321 gcgaatcaag caccaggcca tcttcctgac gctctttgag tacaccttca aagacattaa
    22381 ccgaaccgat aaattcagcg ctgtagcggg tagttggatg ctcgtagatc tcttccggct
    22441 cgccaatctg gacaaatttc ccgcgattca taatggcgat acgccccgcc atggtcatcg
    22501 cctcttcctg atcgtgggtg accatcacac aagtcacacc gacgcgctcc agaatatcca
    22561 ccacttcaag ctgcatccgg tcacgcagct ttttatccag cgcgcccatc ggctcatcga
    22621 gcagtaatag tttcgggcgt ttggcaaggc ttcgggccag ggccacacgc tgtcgctgac
    22681 caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg catgtgcacc aggccgagca
    22741 tctcatggac ccgactggca atttccgctt tcggtagttt gtcctgtttc aggccaaaag
    22801 cgatgttctg ttccacggtc atatgcggaa acagcgcgta agactgaaac atcatattga
    22861 tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc atcgagcatt atctgcccgg
    22921 cagaaggttg ttcaaaacct gccagcatac gcagcagcgt ggacttgcca cagccggatg
    22981 cgcccagcag cgcgaagatt tcacctttgt agatggtcag gctgacatca tcgaccgcat
    23041 gctgaccatc gtaggatttg gtgaggttgc ggatttctaa tagcggcgtc agcgccttac
    23101 gggttttcgc ctgcgggcga gggatagcgt cattcacggc tgctctccgg cattgaatgt
    23161 gtgcaagcgc accatgatgg tgcgaattgc cggctggtgt ggtgcgcccg cccggcatct
    23221 aaggctgcgg attattttcc gctcttcact ttggtccacg cgcgggtgcg cacacggtcg
    23281 attttcggat cctgtacttt cagagtgaat agcttcgcac gaacgtccgc cggaggataa
    23341 atacccgggt tctcacggac ttccgcactc accagcggcg tggctgcttt attggcgttg
    23401 gcatagaaca catggtcgga aatatgcgct actacatccg ggcgcagcag gtaattcagg
    23461 aactgatagg cttcgtcttt gtttttggca tccgcaggca tggcgaatac atcaaagaac
    23521 gccatcgccc cttcttttgg aatcgagaac gagacattca ctccattctt cgcttccttc
    23581 gcgcggtttg acgcctgcca gacatcacct gcccagccga tagcgacgca aatatcgccg
    23641 tttgccaggt cgttaatgta ttgagatgaa tggaaataac gaatgttcgg gcgcagcttt
    23701 aacagcagat cggttgccgg tccggtgtaa tcatccgctt tagtgctgtt gggatctttg
    23761 ccgagataat tcaacacggt agcaaaaact tcttctggcg catccaggaa agagacaccg
    23821 cagcttttca gtttttccag attttcaggt ttgaggatca agtcccagct atcgacgggc
    23881 gcgttttcgc ccagcacagc tttaacttta tcaacgttat agccaatccc ggtcgtcgcc
    23941 cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt tggcgaccag cttcagcagc
    24001 tccgggtcga gattcttcca ctccggcaat ttgcttttgt ccagcggctg gaaaactccc
    24061 gcagtcaact ggcgttcaag aaagctggca gatggaacca ccaagtcaaa gccggtgcta
    24121 cccgccatta atttgccttc cagtacttcg ttagagtcga aaacatcgta gacgacttta
    24181 ataccggttt ctttttcaaa attggccacc gtgtccgggg cgatataatc agaccagtta
    24241 taaatgtgga gtgttttttg ttcagccgcg agcgtgccga cagagacggc catcagagca
    24301 cccgcagcca gacccgaaag ccatttttta tttaaggcgg tcatatctct ttccttctga
    24361 aagttcgtta acaaacgaac aaaagttgtc acactaaaag atatgcaata aacgtgcata
    24421 ttttttatcg gcggggtgta agaaaagaga actctctccc ggccagcact gctcgctata
    24481 taaaagcatc gcaagaataa ctttagccag ggtttgagac tatagctcgg attaaaaaac
    24541 ggtatttaac aaatttttat gcgtatttgc tctatgtgat aaagccaaat cgacacaaga
    24601 gaagtgaaat aatctttaag aaaaggttaa aaacagcaga taattttgca gcgcgcagcc
    24661 actgcaccag cgactgcgtg ctcgaaagga gttatcagtg gaggaaatag ttttcggtaa
    24721 cgttgttttg cccttcatcg tcagtcggta acagcagttg atgggcatta gcttcaagaa
    24781 tgaccatcga aatctgctct tcgctctgac gcacgaacca tgcaaactgc tcataggtta
    24841 cgccctgcat aacggataaa gactggcaaa ccaccagctt tggcagatta tcatcctgca
    24901 tatcaagaaa tgctttcacg gtcagcgaac tggcattgat ggcagataaa tccgccgcca
    24961 gcggcaatac ggctgaaggt cggacttccg ccatggcaga aaacaggatc gtgttatcga
    25021 tcagatcgat tttggcatca aagacaccgt cgaaattctg catatggggc agatgcagag
    25081 cctgacagtt atcacattca aaaaaactca tccccaggtc atcgagccat tgacgcagcg
    25141 tatccagacc aggaacgacc agcgatgtca taattttgta caacctcttt cgataaaaag
    25201 accggcacag cttacgcaaa aagcgcaggc aaaaccatga tcagtaatgt gattgcgact
    25261 aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg ttcgatccag cggatcattt
    25321 tacccgcgat gtcgatacca gtggtttttt ctattccttc cagccccggc gacgcattca
    25381 cttccatcac caacggcccg cgattagcac gcagaatatc cacaccagca acgtccagcg
    25441 ccatcgttcg cgcggctttt atcgcgattt cacgctcctg tggtgtgata ctggcgacgc
    25501 ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc ctctttcgcc cgccgttcaa
    25561 tcgcagcgac cacttcatcg ccaacaacca gacagcggat atcgcaccct tgcgcctctt
    25621 tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg gaaagcgtca atcacgcttt
    25681 ccgccgcctg acgcgtctcc gccagcacga cgccaattcc ctgcgtgcct tcaaccaact
    25741 tgaccaccag cggcgcacca ccgaccatgt cgattaaatc gctggtatca tccggcgaat
    25801 gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc cagcagttgc atggaacgca
    25861 atttgtcacg cgcccgggca atggcgaccg actcattgag cggatagctc cccagcatct
    25921 cgaactggcg cagtgccgcc gtcccataaa aggtaatggc ggtgccaata cgcgggatca
    25981 ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat agaagacgcc gcaggattta
    26041 tgttcatgta gcaagaaagc ggatcaagaa tttcaaccag gtgaccgcgc tgtatagcgg
    26101 cttcacgcag ccgcttacac gaatagagcg ttccatcccg ggacaatatg gcaattttca
    26161 ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta tcatacatcg acgtggcggt
    26221 tttagcgcgt cgcccaaccc tgtttgtgca aataatccag aataaatggg cggctttctt
    26281 taatgattgt tcggcggata tgatcgctcc aggtatcccg gcgattattg ctgccacggg
    26341 tgaggtaata ttccgccagt tgctcgtcat actgcgccag tgcgtcttta tccagcggtt
    26401 gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg cggcttgaga tccggattat
    26461 ccgcaggcca gccaaggcac agcccaaaca gcggcagaac atgctgcggt aatttcagca
    26521 gtttcgtcac cgcttcaata ttattgcgca ggccgccgat atataccccg
//
LOCUS       DAEEBB010000049        26561 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEEBB010000049 DAEEBB010000000
VERSION     DAEEBB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.049"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..164)
                     /locus_tag="KLF07_001905"
     CDS             complement(<1..164)
                     /locus_tag="KLF07_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831930.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWME"
     gene            complement(261..731)
                     /gene="rpsG"
                     /locus_tag="KLF07_001906"
     CDS             complement(261..731)
                     /gene="rpsG"
                     /locus_tag="KLF07_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBD9831931.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(828..1202)
                     /gene="rpsL"
                     /locus_tag="KLF07_001907"
     CDS             complement(828..1202)
                     /gene="rpsL"
                     /locus_tag="KLF07_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBD9831932.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(1328..1615)
                     /gene="tusB"
                     /locus_tag="KLF07_001908"
     CDS             complement(1328..1615)
                     /gene="tusB"
                     /locus_tag="KLF07_001908"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBD9831933.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(1623..1982)
                     /gene="tusC"
                     /locus_tag="KLF07_001909"
     CDS             complement(1623..1982)
                     /gene="tusC"
                     /locus_tag="KLF07_001909"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBD9831934.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(1982..2368)
                     /gene="tusD"
                     /locus_tag="KLF07_001910"
     CDS             complement(1982..2368)
                     /gene="tusD"
                     /locus_tag="KLF07_001910"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBD9831935.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(2368..3090)
                     /locus_tag="KLF07_001911"
     CDS             complement(2368..3090)
                     /locus_tag="KLF07_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9831936.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(3257..4069)
                     /gene="fkpA"
                     /locus_tag="KLF07_001912"
     CDS             complement(3257..4069)
                     /gene="fkpA"
                     /locus_tag="KLF07_001912"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD9831937.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            4290..4508
                     /locus_tag="KLF07_001913"
     CDS             4290..4508
                     /locus_tag="KLF07_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBD9831938.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(4557..5147)
                     /gene="slyD"
                     /locus_tag="KLF07_001914"
     CDS             complement(4557..5147)
                     /gene="slyD"
                     /locus_tag="KLF07_001914"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD9831939.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(5242..5442)
                     /locus_tag="KLF07_001915"
     CDS             complement(5242..5442)
                     /locus_tag="KLF07_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBD9831940.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(5452..7257)
                     /gene="kefB"
                     /locus_tag="KLF07_001916"
     CDS             complement(5452..7257)
                     /gene="kefB"
                     /locus_tag="KLF07_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBD9831941.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(7257..7808)
                     /gene="kefG"
                     /locus_tag="KLF07_001917"
     CDS             complement(7257..7808)
                     /gene="kefG"
                     /locus_tag="KLF07_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBD9831942.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            7939..9852
                     /locus_tag="KLF07_001918"
     CDS             7939..9852
                     /locus_tag="KLF07_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9831943.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            9852..10874
                     /locus_tag="KLF07_001919"
     CDS             9852..10874
                     /locus_tag="KLF07_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD9831944.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            10868..11086
                     /locus_tag="KLF07_001920"
     CDS             10868..11086
                     /locus_tag="KLF07_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBD9831945.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            11140..12009
                     /locus_tag="KLF07_001921"
     CDS             11140..12009
                     /locus_tag="KLF07_001921"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBD9831946.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(12064..12468)
                     /locus_tag="KLF07_001922"
     CDS             complement(12064..12468)
                     /locus_tag="KLF07_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBD9831947.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            12770..13402
                     /gene="crp"
                     /locus_tag="KLF07_001923"
     CDS             12770..13402
                     /gene="crp"
                     /locus_tag="KLF07_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBD9831948.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            13453..15543
                     /locus_tag="KLF07_001924"
     CDS             13453..15543
                     /locus_tag="KLF07_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD9831949.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(15610..16830)
                     /gene="argD/dapC"
                     /locus_tag="KLF07_001925"
     CDS             complement(15610..16830)
                     /gene="argD/dapC"
                     /locus_tag="KLF07_001925"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBD9831950.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(16916..17479)
                     /gene="pabA"
                     /locus_tag="KLF07_001926"
     CDS             complement(16916..17479)
                     /gene="pabA"
                     /locus_tag="KLF07_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBD9831951.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(17511..18113)
                     /locus_tag="KLF07_001927"
     CDS             complement(17511..18113)
                     /locus_tag="KLF07_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBD9831952.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(18103..18270)
                     /locus_tag="KLF07_001928"
     CDS             complement(18103..18270)
                     /locus_tag="KLF07_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBD9831953.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(18375..18947)
                     /gene="ppiA"
                     /locus_tag="KLF07_001929"
                     /gene_synonym="rotA"
     CDS             complement(18375..18947)
                     /gene="ppiA"
                     /locus_tag="KLF07_001929"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBD9831954.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            19218..20399
                     /gene="tsgA"
                     /locus_tag="KLF07_001930"
     CDS             19218..20399
                     /gene="tsgA"
                     /locus_tag="KLF07_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBD9831955.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            20661..23204
                     /gene="nirB"
                     /locus_tag="KLF07_001931"
     CDS             20661..23204
                     /gene="nirB"
                     /locus_tag="KLF07_001931"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBD9831956.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            23201..23527
                     /gene="nirD"
                     /locus_tag="KLF07_001932"
     CDS             23201..23527
                     /gene="nirD"
                     /locus_tag="KLF07_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBD9831957.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            23653..24459
                     /gene="nirC"
                     /locus_tag="KLF07_001933"
     CDS             23653..24459
                     /gene="nirC"
                     /locus_tag="KLF07_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBD9831958.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            24478..25851
                     /gene="cobA"
                     /locus_tag="KLF07_001934"
     CDS             24478..25851
                     /gene="cobA"
                     /locus_tag="KLF07_001934"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD9831959.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            26098..26265
                     /locus_tag="KLF07_001935"
     CDS             26098..26265
                     /locus_tag="KLF07_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBD9831960.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6478 a         7123 c         6676 g         6284 t
ORIGIN      
        1 tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt atggtttaca
       61 ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat gtgcgcactg
      121 ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt cctcgtttat
      181 cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc gcccgcctgg
      241 tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc
      301 atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc agcatcagaa
      361 agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc agcttcaacg
      421 atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg tacctgataa
      481 gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt ttcgagagct
      541 acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc cagcgcgctg
      601 tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt tacaaattta
      661 gccagcagtt ctgatccaaa cttcggatcc ggcagaattt tacgctgacc aatgacgcga
      721 cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc tacgagttta
      781 gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta agccttagga
      841 cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc
      901 gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc
      961 aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg
     1021 aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg ttttttagga
     1081 gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc
     1141 acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc
     1201 attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac gtcctcacac
     1261 aatatgagga cgccgaattt tagggcgatg ccgaaaagat gtcaagaaat atacaacgat
     1321 cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg aaatcagtat
     1381 agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca ataaggtctt
     1441 cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag cggttaccgt
     1501 caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca ctgagcagac
     1561 gcagcagcgc agcaaaatcc gccagccagg gtgaacgatg taatgtgtgc agcataaaag
     1621 cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc atctgcttcg
     1681 agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc gcgcagtgaa
     1741 gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa agtggcaatg
     1801 taatcacgcg ccagcactgc atcgggcttt tgtcctggca gcagctgaaa aacgccatca
     1861 gctataaaga agacagccag atcatcagtt aatgcggaag ttgccagtaa agcatctaaa
     1921 ccttcccgac cagcagctgt accatgaggc gcagtagaaa aaacaaacgc aattcgtttc
     1981 atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg ccaagtccgc
     2041 ttaaggtaaa tcccggctga aggtttgacg aggccagccc cagccttccg gcctccgttt
     2101 catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc acaccatgtt
     2161 gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc ggtgaggtca
     2221 attggttagc gttatagacc ccttcccgat aaaaaaagac gctgcttaac tcatggcctt
     2281 ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta ccgtatgctg
     2341 gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat caccgctttt
     2401 gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt cagcaacctg
     2461 gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca cgatctgacg
     2521 atttttggcg ttattagaaa cattgcgatc ggcatttact tcttcgatgg tgaactccag
     2581 cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac cgacatccgg
     2641 ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa gattcatatt
     2701 gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag tcagggactt
     2761 cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc tatccgctcc
     2821 cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa tcttccggcc
     2881 tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca aagagtgcaa
     2941 aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg cttcgtagga
     3001 tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat ccaactcact
     3061 ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc aggagcctgt
     3121 cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat acagcggagg
     3181 ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg cctggtcagg
     3241 cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca gcttccggct
     3301 ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac accagggtag
     3361 aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt ggaataacca
     3421 gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccacccc gggataacac
     3481 cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac tctttaccgt
     3541 cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt gcttcgcctt
     3601 taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca cctttctctt
     3661 tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg tctttttcca
     3721 tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct
     3781 cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg atcagctgat
     3841 ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc atgtaacgac
     3901 ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac gctgctttgc
     3961 tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt gcatgcaggg
     4021 caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc catatctcca
     4081 ggatcggggc atctcacccc agggttaact attatcagaa gggtactata aagcgttgtc
     4141 gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct ttttgtttaa
     4201 attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa gtagaatccg
     4261 cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa gcacgcctgg
     4321 cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg aacgtcacgg
     4381 tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg ctgaccgaga
     4441 agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg ccaccgccac
     4501 attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt tcggtattag
     4561 tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc gtgttcatga
     4621 ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg gtgatgatcg
     4681 tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg aatcgccaca
     4741 acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc aaccactacg
     4801 tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt ttcagccagg
     4861 aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg aacacgttgc
     4921 accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac atcaaatttg
     4981 tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga accgtgacca
     5041 tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc gtcttctgta
     5101 cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat atctcctgag
     5161 catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac tcactagctt
     5221 aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt gctcatcttt
     5281 gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc acttaacgca
     5341 ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg cctgacaggc
     5401 cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc tctactcaaa
     5461 ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga aaatctcttc
     5521 cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat gcattgggat
     5581 gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc gctgcgcctg
     5641 atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg cactggagaa
     5701 tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc
     5761 gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt ccaccagctt
     5821 catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct ctgcacccgc
     5881 agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc catatttacg
     5941 catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt tatttgccat
     6001 cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga cctggggttt
     6061 atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact ggcgggatag
     6121 ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg tcaccgtcac
     6181 cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg tagaaaagag
     6241 gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc gctctgagct
     6301 acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg ccaccagcac
     6361 aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt tgagcgacat
     6421 gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga tagccgtttc
     6481 cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg taccgagcgc
     6541 catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca gcagcgtcgc
     6601 ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg gacgcagtaa
     6661 atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga tcttcatcca
     6721 gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg ctgggatcac
     6781 tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc tgcgattcat
     6841 ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa ggccaatgcc
     6901 accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc
     6961 cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca gttgccaaag
     7021 tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc cgagttccga
     7081 aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc caattgcaat
     7141 ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca acggcaccgc
     7201 agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac cttccatcag
     7261 cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc gtggctcgcc
     7321 agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa ccaatgcatc
     7381 cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg gtagcgattc
     7441 agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt acgccagtac
     7501 tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa tacccggtcc
     7561 agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg aaagacaatc
     7621 acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa atcgggatag
     7681 tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt aagcagtacc
     7741 cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac tttcgctggc
     7801 tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt gggctaccat
     7861 tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag ttaacatagt
     7921 ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc
     7981 gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg cctggtgggt
     8041 aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat cagcgccgac
     8101 ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca ggaaacgcca
     8161 gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata tcgtcaactg
     8221 gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc gaccattcat
     8281 ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag cctgctgcac
     8341 ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc cggtggctgg
     8401 cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct gctgctcgac
     8461 gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg gttgaagagc
     8521 tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc gatcgttgat
     8581 aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta cagttcgttt
     8641 gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag ccagcaggaa
     8701 cgcgtggcac atctgcaaag ttatatcgac cgtttccgtg ccaaagccac caaagcgaag
     8761 caggcccaga gccgcattaa gatgcttgag cgtatggagc tgattgcccc cgcgcacgtc
     8821 gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag
     8881 atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat taaactgaac
     8941 ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa atcgacatta
     9001 atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg
     9061 atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga cgaatcacct
     9121 attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg tgactacctc
     9181 ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt ctccggtggg
     9241 gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct gctgctgctc
     9301 gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga agcattaatc
     9361 gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg ttccaccact
     9421 gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat
     9481 tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga agcgccaaaa
     9541 gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt
     9601 gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat ggagaagctg
     9661 aacgcgcaac tggcgcaggc ggaagagaaa ctcggagaca gcgaactgta tgaccagagc
     9721 cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa
     9781 gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct gctggaaggc
     9841 caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc agcagtgctg
     9901 aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg ccgcgtttgt
     9961 ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc gacggcgatt
    10021 ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg cgtttagtgg
    10081 tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg gttgaggcgg
    10141 cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa
    10201 accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg tttttacgct
    10261 ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg ctcggcggta
    10321 atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat gcggcggtga
    10381 ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag ggcttttccc
    10441 gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc aagctggcag
    10501 cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt cgcatccgtg
    10561 aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc gactattatc
    10621 gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg attattcacg
    10681 ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt ctccccccgc
    10741 aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc ggtacattac
    10801 ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg tatctggagg
    10861 cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa atttaattga
    10921 aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac ttgaacagaa
    10981 agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat ggtcggagct
    11041 gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaatagcctg caaccgtcgg
    11101 cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa acatccggtc
    11161 attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa
    11221 atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt tcaccgttac
    11281 acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc
    11341 tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat tgaatacggt
    11401 cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc cgtaccgtgg
    11461 aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac tgatgtactg
    11521 ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc gcagcatgtg
    11581 gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa actgatccgc
    11641 gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt gcgttcaatg
    11701 gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa cttccagcgc
    11761 gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc gctcgatgaa
    11821 agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg gctgctggcg
    11881 atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg cggcaaaatg
    11941 ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga aggaaagaaa
    12001 atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa cgggatagtt
    12061 aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt ccagcatcaa
    12121 cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg ctgcgtcttt
    12181 caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc
    12241 ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac gccctttttg
    12301 caggatagaa accacatcga tggcactgca accacccgcc gccatcaaca ccatttccat
    12361 cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac cagaggcgga
    12421 ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt ttgctactcc
    12481 actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac ggaaggcaac
    12541 ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc taaaacagtc
    12601 aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca cattaccgtg
    12661 cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa tccagagaca
    12721 gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca tggtgcttgg
    12781 caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca ttcataagta
    12841 cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt
    12901 taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga tcctctccta
    12961 tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc aggaacgtag
    13021 cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg
    13081 ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga tggcgcgtcg
    13141 tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat
    13201 tgcacagact ctgctgaatc tggcaaaaca accagacgct atgactcacc cggacggtat
    13261 gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt
    13321 gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg gtaaaaccat
    13381 cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta gcgcgccatt
    13441 ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta tgcacttcga
    13501 caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga attacgattc
    13561 ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga tacgcctcat
    13621 aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg tagcttgctg
    13681 acacagctac tactggcaaa agatgttccc ctgccctttt tgctgaccgg attaacgctg
    13741 gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc tgcatcgctg
    13801 ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga accgttgctc
    13861 atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt ctggatctgg
    13921 cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gcgaactggc agattattgt
    13981 gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct gccgccgctg
    14041 ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca aatgcatatg
    14101 ctttccgcgc aaaataatac tgattacaag cggatgctgc gtattttcca ggaggcgctg
    14161 gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca aaagctggtc
    14221 gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg
    14281 cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac gatggaaaag
    14341 caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt tgggcaattc
    14401 tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc
    14461 cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct gaaaagttat
    14521 ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat gttaagcgtt
    14581 gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct gatgacggta
    14641 ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa tcgctccgtg
    14701 ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat tcccgaaggt
    14761 tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt gcgcaaaaac
    14821 tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca actattgtgg
    14881 ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg ttgtttaatt
    14941 gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac
    15001 gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag cgaggatccg
    15061 caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa cactctgtat
    15121 aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct ggcagatatg
    15181 aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat gaccacgctg
    15241 gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca gtcttgtgaa
    15301 atcgccattc agcgttgtca gcagcgactg gaatatgacg aaccggggag ttctggcgat
    15361 gccaatatca tggattcgcc ggagatgcag ccgcacgaag gcgcggcagg tacgctggag
    15421 cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc gtcgatggca
    15481 tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg
    15541 taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct tttgcctgat
    15601 gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccttc
    15661 atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat ccggtccggc
    15721 attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg cccgaccttt
    15781 gtactgtggt ttcagctctg cgccaatcaa cagccccata ccgcgaatat cgctaaacac
    15841 atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt tcgcctgaat
    15901 gccttccagc acttcagggg gattgatgat atcaaacgcc gcccccgcta ctgcacaggc
    15961 cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag aagcaatttc
    16021 cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg cagaggtcag
    16081 aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc gccccatccc
    16141 gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc
    16201 ctgcaaaaac tctggcgtcg cagccgtcac gccgccctcg ccctggatcg gctcaaccac
    16261 caccgcacag gtgtgatcat ccatcactgc tttcactgcg tggagatcgt taaagggaac
    16321 gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct gcccacccac
    16381 cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa ttttggtttt
    16441 gaacgggcta tgacgcacac aggcgtaatg acgtgccagt ttaaaggcgg tttcgttagc
    16501 ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa tcagtttacg
    16561 cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca gaatttcgcc
    16621 ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg taactgcaat
    16681 gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc tgccctgacc
    16741 ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat cgaaagtcgc
    16801 gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg cataaacaaa
    16861 tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc agaaatcagc
    16921 gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt tctggatgga
    16981 actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg cgggtttcgc
    17041 tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc agcgaatggt
    17101 agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg ctatgtgtaa
    17161 tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg ccaaatgcct
    17221 gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg gcatagtgac
    17281 gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt gagatgacaa
    17341 ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca
    17401 gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt
    17461 tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt ttactcagat
    17521 tccccggctt tgcttaccac tttgctaaat atcatctgca gtgcggtgag atcccccatt
    17581 gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca gctcagttga
    17641 tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc acttcccacc
    17701 cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc gacgaacttc
    17761 gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag ggcattgccc
    17821 tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat atcaacttca
    17881 cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat agtgcgcaaa
    17941 tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt
    18001 tcgtaagcgg cctgttccag ccgctgctgc tgacggatgt tcagccggtt acgcatgata
    18061 tcaaggccag gataaagata cggatcgcgc ccttcaccga atttatcgct catagtgcct
    18121 cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta aaggcatctc
    18181 gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct cccagagacg
    18241 ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc
    18301 acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg caagattgcc
    18361 cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg ttttgacggc
    18421 acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc ggcaacgtcc
    18481 atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc atggtcaagg
    18541 aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt gtcagcggta
    18601 cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc atttttgatt
    18661 ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg aatcataaag
    18721 ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac ataatcgaca
    18781 aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat gttaccagct
    18841 gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg agaaagagca
    18901 gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag atttccttaa
    18961 caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag aacaaggcca
    19021 tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac tttttattcc
    19081 gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa acatttaaac
    19141 agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc cattttattt
    19201 ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg attagctttc
    19261 tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg
    19321 ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc ctcaacgccg
    19381 gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc
    19441 agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg ttcagccaca
    19501 gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc attactatgt
    19561 cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt
    19621 tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc gccgcgtttc
    19681 tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg gtgtatgtcg
    19741 ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacat gcaccaaaaa
    19801 cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc tctgttgcgg
    19861 cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag tatgccaaag
    19921 gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg atgtcataca
    19981 tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa cgcattctga
    20041 ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga acaccagcac
    20101 atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat accaccatca
    20161 tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac tttgtcctga
    20221 cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt gttgaacata
    20281 gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt
    20341 gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc tctcattaat
    20401 tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc cgctacgtgc
    20461 atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga aatgtcagac
    20521 aattccgtga cttaagaaaa tttatacaaa tcagcaacat accacttaag gagtatataa
    20581 aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta ggcggtaata
    20641 gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt aacggtatgg
    20701 tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac tttgatatta
    20761 ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg tcttacttct
    20821 ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca
    20881 tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag gtgattcact
    20941 ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt tcctacccgt
    21001 ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc accattgaag
    21061 acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt gttggtggcg
    21121 gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa acccacgtta
    21181 tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc gagcagctgc
    21241 gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg
    21301 tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc gaactggaag
    21361 tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca acccagtgtg
    21421 gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag acttccgatc
    21481 cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag
    21541 cacctggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc gaaaacgcct
    21601 ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta ggcggtattg
    21661 gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac gaaagtaaag
    21721 agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt gcggtactgg
    21781 tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct atcgaactgc
    21841 cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag ccgtctatcg
    21901 gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc actaaaggtg
    21961 atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa gctgaaacca
    22021 aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg aacgcggaac
    22081 tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct tattcgcgtc
    22141 aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga
    22201 aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct
    22261 cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat tctaacgaca
    22321 acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt tctccgggcg
    22381 gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa tttaatctct
    22441 acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc
    22501 cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc tatgcgaaag
    22561 cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt gttggcgata
    22621 gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa
    22681 tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt aaagacgtgg
    22741 gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac
    22801 cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc aaatatctcg
    22861 accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc gcgccgtggt
    22921 tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac gacaaactgg
    22981 ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt
    23041 ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc atcaacagcg
    23101 acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt ccggcaacgc
    23161 cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt ggaaagacat
    23221 ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca
    23281 ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca acattgatcc
    23341 gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg gcgagctgtg
    23401 ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt gcatggaaga
    23461 cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg tgcagctgcg
    23521 cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc tgtaatgacc
    23581 ttcggtatat tgcagaacat tttttaaact ttttgtttta ttttttgttt ttatttttta
    23641 aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc tgcgcgcatt
    23701 gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat ggcgggcgca
    23761 tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga cccgtccgta
    23821 cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat tatcgccggt
    23881 tctgaactgt tcaccggaca cgccatgttc ctcacctttg gggtaaaagc gggcagcatc
    23941 agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct ggtcggttcc
    24001 gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt agataccagc
    24061 atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact gttcttcaaa
    24121 ggtgcattgt gtaactggct ggtttgcctg gctatctgga tggcgctgcg cactgaaggg
    24181 gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag
    24241 cactccatcg ctaacatgac gctgttcgcc ctctcctggt tcggcaacca cagcgaagcc
    24301 tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa tactttatcg
    24361 ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt
    24421 gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg tttttacgtg
    24481 gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt cggcggtggt
    24541 gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt aacggtgaat
    24601 gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt aaccctcgtc
    24661 gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc agcgacggat
    24721 gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac
    24781 gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga ccgctcaccg
    24841 ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa
    24901 aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc cgggcaatta
    24961 cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt ctgggagaaa
    25021 ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa agccattact
    25081 gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt
    25141 ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca aattcagcag
    25201 gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct ggtacgccgc
    25261 gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc gcaggaagag
    25321 attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggt
    25381 ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg caacgcgggt
    25441 attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc ctattcgggt
    25501 attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg acacttaaaa
    25561 accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct ggtgttctat
    25621 atgggattga atcaggccgc gactattcag caaaagctga ttgaacacgg tatgcctggc
    25681 gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt gattgacggt
    25741 acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct aattattatt
    25801 ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta atttaacccg
    25861 ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa taaaacataa
    25921 ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga cttacaaaat
    25981 taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa cgagcagtct
    26041 gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg aatgtaaatg
    26101 aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac tgcatgtact
    26161 ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca cccggcaatt
    26221 tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc tgacagaaat
    26281 cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg tatttgtttc
    26341 tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat ttgataacta
    26401 catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc acattacata
    26461 acatcatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat gactgcaaac
    26521 tctcccctac aacgtattgg acaagaaaaa ggtatcgcta t
//
LOCUS       DAEEBB010000050        25773 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEEBB010000050 DAEEBB010000000
VERSION     DAEEBB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.050"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="KLF07_001936"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="KLF07_001936"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBD9831961.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="KLF07_001937"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="KLF07_001937"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBD9831962.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="KLF07_001938"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="KLF07_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBD9831963.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="KLF07_001939"
     CDS             2440..3063
                     /locus_tag="KLF07_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBD9831964.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="KLF07_001940"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="KLF07_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBD9831965.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="KLF07_001941"
     CDS             complement(3447..3512)
                     /locus_tag="KLF07_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831966.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="KLF07_001942"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="KLF07_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBD9831967.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="KLF07_001943"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="KLF07_001943"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBD9831968.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="KLF07_001944"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="KLF07_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBD9831969.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="KLF07_001945"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="KLF07_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBD9831970.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="KLF07_001946"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="KLF07_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBD9831971.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="KLF07_001947"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="KLF07_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBD9831972.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="KLF07_001948"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="KLF07_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBD9831973.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="KLF07_001949"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="KLF07_001949"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBD9831974.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="KLF07_001950"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="KLF07_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBD9831975.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="KLF07_001951"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="KLF07_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBD9831976.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="KLF07_001952"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="KLF07_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBD9831977.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="KLF07_001953"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="KLF07_001953"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBD9831978.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="KLF07_001954"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="KLF07_001954"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBD9831979.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="KLF07_001955"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="KLF07_001955"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBD9831980.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="KLF07_001956"
     CDS             23127..23711
                     /locus_tag="KLF07_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9831981.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="KLF07_001957"
     CDS             23816..24556
                     /locus_tag="KLF07_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD9831982.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25773
                     /gene="htrE"
                     /locus_tag="KLF07_001958"
                     /pseudo
     CDS             24591..25773
                     /gene="htrE"
                     /locus_tag="KLF07_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6983 c         6416 g         6044 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgtcccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aac
//
LOCUS       DAEEBB010000051        25647 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEEBB010000051 DAEEBB010000000
VERSION     DAEEBB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.051"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="KLF07_001959"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="KLF07_001959"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBD9831983.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="KLF07_001960"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="KLF07_001960"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBD9831984.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="KLF07_001961"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="KLF07_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBD9831985.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="KLF07_001962"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="KLF07_001962"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBD9831986.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="KLF07_001963"
     CDS             complement(5499..5696)
                     /locus_tag="KLF07_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBD9831987.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="KLF07_001964"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="KLF07_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBD9831988.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="KLF07_001965"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="KLF07_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBD9831989.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="KLF07_001966"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="KLF07_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBD9831990.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="KLF07_001967"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="KLF07_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBD9831991.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="KLF07_001968"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="KLF07_001968"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBD9831992.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="KLF07_001969"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="KLF07_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBD9831993.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="KLF07_001970"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="KLF07_001970"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBD9831994.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="KLF07_001971"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="KLF07_001971"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBD9831995.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="KLF07_001972"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="KLF07_001972"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBD9831996.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="KLF07_001973"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="KLF07_001973"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBD9831997.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLLGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="KLF07_001974"
     CDS             complement(17138..17191)
                     /locus_tag="KLF07_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9831998.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="KLF07_001975"
     tRNA            complement(17425..17500)
                     /locus_tag="KLF07_001975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="KLF07_001976"
     tRNA            complement(17536..17611)
                     /locus_tag="KLF07_001976"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="KLF07_001977"
     tRNA            complement(17769..17844)
                     /locus_tag="KLF07_001977"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="KLF07_001978"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="KLF07_001978"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBD9831999.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="KLF07_001979"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="KLF07_001979"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBD9832000.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="KLF07_001980"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="KLF07_001980"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBD9832001.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="KLF07_001981"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="KLF07_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBD9832002.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="KLF07_001982"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="KLF07_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBD9832003.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7193 c         6499 g         6021 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat tactaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAEEBB010000052        25503 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEEBB010000052 DAEEBB010000000
VERSION     DAEEBB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.052"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            15..485
                     /locus_tag="KLF07_001983"
     CDS             15..485
                     /locus_tag="KLF07_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBD9832004.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            487..906
                     /gene="gspB"
                     /locus_tag="KLF07_001984"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="KLF07_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBD9832005.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="KLF07_001985"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="KLF07_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBD9832006.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="KLF07_001986"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="KLF07_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBD9832007.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="KLF07_001987"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="KLF07_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBD9832008.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="KLF07_001988"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="KLF07_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBD9832009.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="KLF07_001989"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="KLF07_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBD9832010.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="KLF07_001990"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="KLF07_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBD9832011.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="KLF07_001991"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="KLF07_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBD9832012.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="KLF07_001992"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="KLF07_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBD9832013.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="KLF07_001993"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="KLF07_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBD9832014.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="KLF07_001994"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="KLF07_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBD9832015.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="KLF07_001995"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="KLF07_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBD9832016.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="KLF07_001996"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="KLF07_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBD9832017.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="KLF07_001997"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="KLF07_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBD9832018.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="KLF07_001998"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="KLF07_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBD9832019.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="KLF07_001999"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="KLF07_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBD9832020.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="KLF07_002000"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="KLF07_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBD9832021.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="KLF07_002001"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="KLF07_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBD9832022.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="KLF07_002002"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="KLF07_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBD9832023.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="KLF07_002003"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="KLF07_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBD9832024.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="KLF07_002004"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="KLF07_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBD9832025.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="KLF07_002005"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="KLF07_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBD9832026.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="KLF07_002006"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="KLF07_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBD9832027.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="KLF07_002007"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="KLF07_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBD9832028.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="KLF07_002008"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="KLF07_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBD9832029.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="KLF07_002009"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="KLF07_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBD9832030.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="KLF07_002010"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="KLF07_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBD9832031.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="KLF07_002011"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="KLF07_002011"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBD9832032.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="KLF07_002012"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="KLF07_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBD9832033.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="KLF07_002013"
     CDS             14905..15273
                     /locus_tag="KLF07_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBD9832034.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="KLF07_002014"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="KLF07_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBD9832035.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="KLF07_002015"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="KLF07_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBD9832036.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="KLF07_002016"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="KLF07_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBD9832037.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="KLF07_002017"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="KLF07_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBD9832038.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="KLF07_002018"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="KLF07_002018"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBD9832039.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="KLF07_002019"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="KLF07_002019"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBD9832040.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="KLF07_002020"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="KLF07_002020"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBD9832041.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="KLF07_002021"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="KLF07_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBD9832042.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /locus_tag="KLF07_002022"
     CDS             21950..22423
                     /locus_tag="KLF07_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBD9832043.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="KLF07_002023"
     CDS             22452..22994
                     /locus_tag="KLF07_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBD9832044.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="KLF07_002024"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="KLF07_002024"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBD9832045.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="KLF07_002025"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="KLF07_002025"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBD9832046.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="KLF07_002026"
     CDS             24391..24648
                     /locus_tag="KLF07_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBD9832047.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="KLF07_002027"
     CDS             complement(24624..25178)
                     /locus_tag="KLF07_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBD9832048.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6028 c         6682 g         6301 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    25501 cgc
//
LOCUS       DAEEBB010000053        25429 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEEBB010000053 DAEEBB010000000
VERSION     DAEEBB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.053"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="KLF07_002028"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="KLF07_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBD9832049.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="KLF07_002029"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="KLF07_002029"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBD9832050.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="KLF07_002030"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="KLF07_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="KLF07_002031"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="KLF07_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBD9832051.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="KLF07_002032"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="KLF07_002032"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBD9832052.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="KLF07_002033"
     CDS             complement(6490..6846)
                     /locus_tag="KLF07_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD9832053.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="KLF07_002034"
     CDS             complement(6850..7266)
                     /locus_tag="KLF07_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBD9832054.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="KLF07_002035"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="KLF07_002035"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBD9832055.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="KLF07_002036"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="KLF07_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="KLF07_002037"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="KLF07_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBD9832056.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="KLF07_002038"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="KLF07_002038"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBD9832057.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="KLF07_002039"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="KLF07_002039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD9832058.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="KLF07_002040"
     CDS             13294..14277
                     /locus_tag="KLF07_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBD9832059.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="KLF07_002041"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="KLF07_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBD9832060.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="KLF07_002042"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="KLF07_002042"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBD9832061.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17204)
                     /locus_tag="KLF07_002043"
                     /pseudo
     CDS             complement(16219..17204)
                     /locus_tag="KLF07_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17522..18037
                     /gene="zur"
                     /locus_tag="KLF07_002044"
     CDS             17522..18037
                     /gene="zur"
                     /locus_tag="KLF07_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBD9832062.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18079..18288)
                     /locus_tag="KLF07_002045"
     CDS             complement(18079..18288)
                     /locus_tag="KLF07_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBD9832063.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="KLF07_002046"
     CDS             complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="KLF07_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBD9832064.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="KLF07_002047"
     CDS             complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="KLF07_002047"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBD9832065.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="KLF07_002048"
     CDS             complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="KLF07_002048"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBD9832066.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21059..23482
                     /gene="plsB"
                     /locus_tag="KLF07_002049"
     CDS             21059..23482
                     /gene="plsB"
                     /locus_tag="KLF07_002049"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBD9832067.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="KLF07_002050"
     CDS             complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="KLF07_002050"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBD9832068.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="KLF07_002051"
     CDS             complement(24522..25019)
                     /gene="ubiC"
                     /locus_tag="KLF07_002051"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBD9832069.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25242..>25429)
                     /locus_tag="KLF07_002052"
     CDS             complement(25242..>25429)
                     /locus_tag="KLF07_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832070.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6579 a         6542 c         6089 g         6219 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcgggt
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagtgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccccagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgcagcgctg cataatcaat agatttacca tatatattat ttaatttacc tttgattgaa
    17101 cctctattat ctaatttatc tttgattgca tcttcattat cgaattgatt tgaattgagt
    17161 gttttattaa agtatccagt tgcaccgggt attatcttca acatgattca tctacctttt
    17221 ttgtcgtacc gtgtcctcct tataattctt ttgccatctg gtcactatca aaaatcgctc
    17281 ataatttctg aactacaggt atgcgtttaa acccagttaa ataaatagcg agttaacttg
    17341 aagcaattta tttaaaaatg aattattaat tattatcgtg ttgataatgg tacgacaagg
    17401 caacataaca ccttacctac aaaacagaaa atcaacatta cggcaacaat aagggttctc
    17461 ggtgtttttc ctggaacatg gtaaagtaag gacattctta acccccactt tgaggtgccc
    17521 gatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcgcagcg
    17581 taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc ctgatgagtc tgcaggatgg
    17641 cgctatcagc gcttatgatc tgcttgattt actgcgcgaa gctgaaccgc aagccaagcc
    17701 gccaacggtt tatcgcgcgc tggattttct gcttgagcaa ggttttgtgc ataaggtgga
    17761 atccaccaac agttatgtgc tctgtcatct gttcgaccag cccacgcata cgtcagccat
    17821 gtttatatgc gatcgctgcg gcgcggtgaa agaagagtgt gcagaaggcg tggaagacat
    17881 tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg cataatgtga ttgaagcaca
    17941 tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt catcctgaac agtgccagca
    18001 tgatcactct gtgcaggtga aaaagaaacc gcgttaagag ggcgtacatc cttgtacacg
    18061 tcgggcagga gggattaatt accagcgata ttcattgcgg gtttcccaat ccacgacctc
    18121 tttttctgcc tgatctttct gataaccata acgttcctgg attttaccga ccagttgatc
    18181 acgtttacct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    18241 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    18301 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    18361 tcagcaattt ggcttaggat aatttattca gaatttttaa ccgtcacgtt gcggcaaacc
    18421 aggtaccgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgcaacgcca
    18481 gaaagacggt tagtgccagc cacaaaccat gattacccag ccacggcagc gtaaggagcg
    18541 tcagcgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    18601 cgcctataaa catgccgtcc agcagataac accagacgcc aaccaacggc aaaatcacct
    18661 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    18721 tgatgtgttc ccctgccagc aaataaacca ccgaaaatag taacgctacg atccccgact
    18781 ggcggcacgc tgcccgccag acatccagca actggctacc gtcgcgcgca ccgtatgcct
    18841 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    18901 gtagcgtcat cagaaccgcg tttacagcga taatgtcact ccccagtcgc gcgccaagta
    18961 cggtgatcgc gccgaaacag agttgcaaca acagcgaacg cagcatgata tcgcggttaa
    19021 gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt cgacatttcg ccggaaattc
    19081 cgcgtagttt gaggatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    19141 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc actagccaga
    19201 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    19261 attgcacgcc gagtaaccaa ccgagtaata ccagattcgc cagcgacgcc ggtgcgctta
    19321 accagcggat ttcaagaaag cgccgcgcct gttccaggac tgcttcacta ccgccaacaa
    19381 tatgcagagc cagatcgata atcggtgtac gcagcagcgc aattaacgcc ccagccccca
    19441 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    19501 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacagc ataaagagaa
    19561 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccgggcta tcaagatgac
    19621 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    19681 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    19741 tgatgaagag agatgaaaaa caaaccgcga cgcctggcgg catcgcggtc tcagagatat
    19801 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    19861 gctgacgaag gtcaacgaca attggtttaa actcgctatt ttctggcaac agttcgactt
    19921 tattaccctg ttttttcagg cgcttaacgg taacttcgtc atcaatacgt gcgacaacga
    19981 cctgaccgtt acgtacatcc tgagttttat gcactgccag caagtcacca tccataatgc
    20041 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    20101 aggaaggatc gacctgataa tgaccttcaa tatgctgctg cgccagaagc ggttcaccgg
    20161 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaacaga cgaatcccgc
    20221 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    20281 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    20341 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    20401 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    20461 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    20521 tatccaaaat gcgaccataa caggatgcac caggttatca cggcgacgat aatcgcaatc
    20581 agcaccgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    20641 attcggtcaa ccactgcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    20701 acggagctga taagcagcac gcgggtaatt gcgtccacat ccagccagca ggcgatgacc
    20761 accgccaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    20821 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    20881 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    20941 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    21001 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    21061 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    21121 aagcaagtct attccggcag atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    21181 tatgtacgtt ttaccgtaca actcgaaagc agatttgctg acgttacgcg cccagtgtct
    21241 ggctcatgac ttgcctgacc cgttagagcc gctagaaatc gacggcacgc tactgccgcg
    21301 ctatgtgttc attcacggcg ggccgcgtgt attcacctat tacacgccga aagaagagtc
    21361 tattaagctg ttccacgact atctcgattt gcaccgtagc aacccaaatc tggatgtgca
    21421 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtaaa
    21481 cccgccgctg cgtatgctta acggcgtaca gaaatttttc gccgtactgt ggctcggtcg
    21541 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    21601 cacggataaa actatcgcac agaaactggc gcgcgtggcg cgtatgcact ttgcccgtca
    21661 acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    21721 cgcctcccgc gccattgcca aagcggtaga agatgaagcg cgcagcaaaa aaatctccca
    21781 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    21841 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttatcaggg
    21901 catcaacgtc cataacgccg agcgcgttcg ccagctggcc cacgacggtc atgagctggt
    21961 gtatgtgcct tgccaccgca gtcacatgga ctacctgcta ctttcttacg tgctgtacca
    22021 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggccgg
    22081 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acttttaaag gcaataaact
    22141 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    22201 gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg ctggatccga aaaccggtac
    22261 gctgtcgatg accattcagg cgatgctgcg tggcggcacg cgtccgatta cgctgattcc
    22321 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    22381 cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc ggtttaagca agctgcgtaa
    22441 tctcggtcag ggttacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    22501 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    22561 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    22621 ggcaaacgcc atgaacctgt gttgtactgc gcttctggca tcacgtcagc gctcactcac
    22681 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    22741 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    22801 aatgaacaag tttgaagtcg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    22861 gcaagcggtg ctgatgacct actatcgcaa caacattgcg catatgttgg tgctgccttc
    22921 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    22981 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    23041 gttgccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    23101 gcaagatgat gagttgcata tcaacccggc gcattctcgc cccctacagc tgctggccgc
    23161 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggttgttga gtgccaaccc
    23221 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gttgcgcaac gtctctccgt
    23281 gctgcacggc atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    23341 gacgctgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    23401 gaaggtttat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    23461 tgcgacgcag ggcgaagggt aatcagagag aattgccgga tgcggcgaaa acgccttatc
    23521 cggccttcca taatctgcaa attcaataaa ttgcgatgtg ttgtgtaggc ctgagaagcg
    23581 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    23641 aaatgccagt aactcattgc cagtcctaaa aacagtacca gaccaacata gttattattc
    23701 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    23761 aacagcgcgc cagccaccag aattgaccag taatatcccc agcctaagcc atttaactca
    23821 ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa taccaataat caatttatcg
    23881 tattggccga acaggattgc ggtggattta ataccaatct tcacatcatc gtcacggtca
    23941 accattgcat actgcgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    24001 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    24061 aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc gcttcataaa cgggtacacc
    24121 cacgccaacg ccagagcggc aatcgacaac agaatggtca tcgtattcag cgtcagcacc
    24181 agtaaaaatg aaatcaatac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    24241 gcgccgctgg gaagcggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    24301 gcataatcat tcaccacaca tccggcagcg cgcatcagcc agacacccgc gacaaacacc
    24361 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    24421 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    24481 agcttattct gcgtcagact ccactccata tttttttcct cttagtacaa cggtgacgcc
    24541 ggtaaaaaca gttctgttag caacagcggt ttaccgctta atcgcaggcg ggaacgtcgc
    24601 ccccacagtc cggcatcacg gcctatctca ataaagtccc gggttaatgt cgatgatgtg
    24661 aacagatagc gtcctagcgg cgttttaccc aatttttgta acgccagctc cggcccgctt
    24721 aacgttgaca caggaacgac ggtacgaccg gcaagccacg gttcaccatc ggcacataac
    24781 aaaatttcac gtaaccagta acgagactct ttcggcagca gcggcagttc ttcggggatt
    24841 tcattctgct cgacaaaccc ttcgcggatc atcgtcacgc tcaccgtttt tccctgctgt
    24901 tcaaaacgtt ttgtcatgga atcttccagc aacagccagt cgagcagttg cgaatccagg
    24961 gcagggatct ctttaaaata gcgcagcgca cgcagttgcg ttaacgcggg gtgtgacatg
    25021 ccgaactctc cgttacataa cgtaaaggca ttgtatcgca gaaaagtgtt tacgggagat
    25081 gtaaagcaaa ttaagtgatc taatgcgcaa caaaaacgca acaatcgcat gaagtggtaa
    25141 aacgctgttg aaccttacgc tttgccgatg cccgtgctgg cgagggcaac cgtaggggca
    25201 tcaccgccca tgatgccagg aacggtgatg ttttccttta cttaaatacc tatattcttt
    25261 atacgagtag ctcgccaact cgcagtaaaa attgggaaaa gcgcatattt accatgagat
    25321 ttggagctaa aaagtgtttc ctgagtaaga ttaataggca acatcttatg tctggattta
    25381 tagatacacg tggaattcca attggtatct tttgtaggat tcaacatat
//
LOCUS       DAEEBB010000054        25129 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEEBB010000054 DAEEBB010000000
VERSION     DAEEBB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25129
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.054"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(166..576)
                     /locus_tag="KLF07_002053"
     CDS             complement(166..576)
                     /locus_tag="KLF07_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBD9832071.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="KLF07_002054"
     CDS             complement(634..867)
                     /locus_tag="KLF07_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBD9832072.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="KLF07_002055"
     CDS             1256..1801
                     /locus_tag="KLF07_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBD9832073.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="KLF07_002056"
     CDS             1776..3701
                     /locus_tag="KLF07_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBD9832074.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="KLF07_002057"
     CDS             3698..3904
                     /locus_tag="KLF07_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBD9832075.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="KLF07_002058"
     CDS             3901..5502
                     /locus_tag="KLF07_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBD9832076.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="KLF07_002059"
     CDS             5483..6802
                     /locus_tag="KLF07_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBD9832077.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="KLF07_002060"
     CDS             6812..7144
                     /locus_tag="KLF07_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBD9832078.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="KLF07_002061"
     CDS             7200..8225
                     /locus_tag="KLF07_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBD9832079.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="KLF07_002062"
     CDS             8267..8665
                     /locus_tag="KLF07_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD9832080.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="KLF07_002063"
     CDS             8677..9030
                     /locus_tag="KLF07_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBD9832081.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="KLF07_002064"
     CDS             9042..9620
                     /locus_tag="KLF07_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9832082.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="KLF07_002065"
     CDS             9617..10012
                     /locus_tag="KLF07_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9832083.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="KLF07_002066"
     CDS             10020..10760
                     /locus_tag="KLF07_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9832084.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="KLF07_002067"
     CDS             10776..11198
                     /locus_tag="KLF07_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBD9832085.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="KLF07_002068"
     CDS             11180..11614
                     /locus_tag="KLF07_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBD9832086.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="KLF07_002069"
     CDS             11607..14168
                     /locus_tag="KLF07_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD9832087.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="KLF07_002070"
     CDS             14165..14494
                     /locus_tag="KLF07_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9832088.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="KLF07_002071"
     CDS             14494..15192
                     /locus_tag="KLF07_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBD9832089.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="KLF07_002072"
     CDS             15198..15941
                     /locus_tag="KLF07_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9832090.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="KLF07_002073"
     CDS             15878..16510
                     /locus_tag="KLF07_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBD9832091.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="KLF07_002074"
     CDS             16571..20068
                     /locus_tag="KLF07_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBD9832092.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="KLF07_002075"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="KLF07_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..22124
                     /locus_tag="KLF07_002076"
                     /pseudo
     CDS             20720..22124
                     /locus_tag="KLF07_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            22073..22225
                     /locus_tag="KLF07_002077"
                     /pseudo
     CDS             22073..22225
                     /locus_tag="KLF07_002077"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22202..22381
                     /locus_tag="KLF07_002078"
                     /pseudo
     CDS             22202..22381
                     /locus_tag="KLF07_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23096..23803
                     /locus_tag="KLF07_002079"
                     /pseudo
     CDS             23096..23803
                     /locus_tag="KLF07_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23803..24384
                     /locus_tag="KLF07_002080"
     CDS             23803..24384
                     /locus_tag="KLF07_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD9832093.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         6040 a         6198 c         7554 g         5337 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaacccgc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca
    23941 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat
    24001 ctcgttgaag atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt
    24061 atttctgaac tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt
    24121 cagaagtgga acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc
    24181 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
    24241 cttcaggatg ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct
    24301 tggaaaaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa
    24361 tggccagtag cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt
    24421 taaaaaagtg attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc
    24481 catgaaagag cttcatggcg agtacataaa cgtacaaaat gtgatgaaag tagagtggcg
    24541 taagccacta tattaagata tgacaatagc gaagaaaagt aaataacatt ttatggacgt
    24601 ttaattgtta attataaaaa ctagagtgcc cgatattgtt tatcttgctt taaaataatg
    24661 tactcaaacc aaatttttga gctaaaagct caaataatat aaatgcaata ataatgttta
    24721 ataagacaat tagcttgctt tgtattttca tttcggaata ctgttaagta actttgatga
    24781 ccctaacaat agatcggaat tttcttagag tcaatggtca ctgtttttgc ttgcaaagca
    24841 aatgttttgg tggttataag tcgtcttaat atagtcacgc atgcaacttg ttccatgcgg
    24901 agattaaaga ttggaatata acgcgaagat ggaattaaat tacatttgca ataattaatt
    24961 tattattact caataattac cgatattatt attgtaaatt aatcattaga atgcaaaaag
    25021 aaatgtaacg gcaaaataag aggacgatag ctgagaaaaa agcgtgcttt cccaatctgg
    25081 ggcaaatcac gtctgaatga cacctgaaaa caggtggtag cctcaagaa
//
LOCUS       DAEEBB010000055        25104 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEEBB010000055 DAEEBB010000000
VERSION     DAEEBB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.055"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            194..382
                     /locus_tag="KLF07_002081"
     CDS             194..382
                     /locus_tag="KLF07_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBD9832094.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="KLF07_002082"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="KLF07_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBD9832095.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="KLF07_002083"
     CDS             1143..1307
                     /locus_tag="KLF07_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBD9832096.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="KLF07_002084"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="KLF07_002084"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBD9832097.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="KLF07_002085"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="KLF07_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBD9832098.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="KLF07_002086"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="KLF07_002086"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBD9832099.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="KLF07_002087"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="KLF07_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="KLF07_002088"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="KLF07_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBD9832100.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="KLF07_002089"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="KLF07_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBD9832101.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="KLF07_002090"
     CDS             14457..15953
                     /locus_tag="KLF07_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD9832102.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="KLF07_002091"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="KLF07_002091"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBD9832103.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="KLF07_002092"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="KLF07_002092"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBD9832104.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="KLF07_002093"
     CDS             18047..20107
                     /locus_tag="KLF07_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD9832105.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="KLF07_002094"
     CDS             complement(20289..21611)
                     /locus_tag="KLF07_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBD9832106.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="KLF07_002095"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="KLF07_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBD9832107.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="KLF07_002096"
     CDS             complement(23084..23983)
                     /locus_tag="KLF07_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9832108.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="KLF07_002097"
     CDS             24503..>25104
                     /locus_tag="KLF07_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD9832109.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAEEBB010000056        23844 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEEBB010000056 DAEEBB010000000
VERSION     DAEEBB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.056"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(70..1323)
                     /gene="amiC"
                     /locus_tag="KLF07_002098"
     CDS             complement(70..1323)
                     /gene="amiC"
                     /locus_tag="KLF07_002098"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBD9832110.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1555..2886
                     /gene="argA"
                     /locus_tag="KLF07_002099"
     CDS             1555..2886
                     /gene="argA"
                     /locus_tag="KLF07_002099"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBD9832111.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2948..4774)
                     /gene="recD"
                     /locus_tag="KLF07_002100"
     CDS             complement(2948..4774)
                     /gene="recD"
                     /locus_tag="KLF07_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBD9832112.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4774..8316)
                     /gene="recB"
                     /locus_tag="KLF07_002101"
     CDS             complement(4774..8316)
                     /gene="recB"
                     /locus_tag="KLF07_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBD9832113.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8309..11197)
                     /gene="ptrA"
                     /locus_tag="KLF07_002102"
     CDS             complement(8309..11197)
                     /gene="ptrA"
                     /locus_tag="KLF07_002102"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBD9832114.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11373..14741)
                     /gene="recC"
                     /locus_tag="KLF07_002103"
     CDS             complement(11373..14741)
                     /gene="recC"
                     /locus_tag="KLF07_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBD9832115.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14754..15077)
                     /locus_tag="KLF07_002104"
     CDS             complement(14754..15077)
                     /locus_tag="KLF07_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBD9832116.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15062..15469)
                     /locus_tag="KLF07_002105"
     CDS             complement(15062..15469)
                     /locus_tag="KLF07_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBD9832117.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15466..16029)
                     /locus_tag="KLF07_002106"
     CDS             complement(15466..16029)
                     /locus_tag="KLF07_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBD9832118.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16020..16490)
                     /locus_tag="KLF07_002107"
     CDS             complement(16020..16490)
                     /locus_tag="KLF07_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBD9832119.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16675..17469)
                     /gene="thyA"
                     /locus_tag="KLF07_002108"
     CDS             complement(16675..17469)
                     /gene="thyA"
                     /locus_tag="KLF07_002108"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBD9832120.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17476..18351)
                     /gene="lgt"
                     /locus_tag="KLF07_002109"
     CDS             complement(17476..18351)
                     /gene="lgt"
                     /locus_tag="KLF07_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBD9832121.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18502..20748)
                     /gene="ptsP"
                     /locus_tag="KLF07_002110"
     CDS             complement(18502..20748)
                     /gene="ptsP"
                     /locus_tag="KLF07_002110"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD9832122.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20761..21291)
                     /gene="rppH"
                     /locus_tag="KLF07_002111"
     CDS             complement(20761..21291)
                     /gene="rppH"
                     /locus_tag="KLF07_002111"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBD9832123.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            21976..22665
                     /gene="mutH"
                     /locus_tag="KLF07_002112"
     CDS             21976..22665
                     /gene="mutH"
                     /locus_tag="KLF07_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBD9832124.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22734..23447
                     /locus_tag="KLF07_002113"
     CDS             22734..23447
                     /locus_tag="KLF07_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD9832125.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23585..23803
                     /gene="ygdR"
                     /locus_tag="KLF07_002114"
     CDS             23585..23803
                     /gene="ygdR"
                     /locus_tag="KLF07_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBD9832126.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5698 a         6527 c         5960 g         5659 t
ORIGIN      
        1 tgaacaccct aacgggtgtt tttttgtttc tactctccca taaaaaaagc gccattcagc
       61 gcctttttat catccccttc tcgccagcgt tgccccatcg gcaaaatacg ctttaatccc
      121 cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc gttttcagtt tacgctcttc
      181 ctcaacgtta ctgataaacg ccgtttcgac cagaatggag ggaatatctg gtgcctttag
      241 tacggcaaac ccggcctgtt caacttgatt tttatgcagc ttgttgattt taccgagctt
      301 attcagcact gctttaccaa acttaaggct gtcagcaatg gtcagcgact gcaccatatc
      361 gaacatggtg tggtcgacat agcggtcacc gcttttgctc acgccaccaa tcaagtccga
      421 ggcgttctgg gtttgtgcca gatattttgc cgcagtactg gttgcacctt tggttgagag
      481 cgcaaacacc gaggaaccgc tcggctggcg actggtaaag gcgtcggcat ggatagagac
      541 aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc acttgcaacg gaatgaagat
      601 gtcttcattg cgcgtcatgt acaccttcat attgccctct ttctcgatca gagagcgcaa
      661 acggcgagct atttgcaata ccacatcttt ttcgcgcgtt ttgtatttcc ccaccgcacc
      721 ggagtcttcg ccaccgtggc cagggtcaag cataatgaca atcggacgat cgcgccctgc
      781 tttacctggt tgtggaccac tttgcgctgg cggcacctgc ttttcgaggt cgcctttgtt
      841 gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc tgtgcattgg ccggatagag
      901 gtccatcacc agacgctctt taaacccggc gaccggcgca agggcaaaca gctgcggttt
      961 tacgttttgc tttaattcaa aaaccatccg tacggtttgc gggtcaaatt gcccgacgcg
     1021 cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc gccatcccct tgagtaccga
     1081 gttcaggttt acatcttcga tatccaccac cacgcgttca gggttactca acgcgaactg
     1141 cttatatttc agctgacgat ttgattctac cgtcacgcgg gtgtagctgg acgcaggcca
     1201 gacgcgcacc gccacgacct ggctaaccgc agccaggctg acctgactta cgctcaatag
     1261 ccacatagcg cccgcgcctt gcagtaaacg acgacggctg attgcagtgt tggatcctga
     1321 catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca agtaaacgac taatttgacc
     1381 ggaaacttta gcgaaagacg cataatctgt catctaataa acggtaaaca ttctttttat
     1441 attcacggca ttactgataa aaaagtcgct ctcgcataaa atttacactt gcaccctgcg
     1501 aaaaaacaga ataaaaatac aataatttcg aataatcatg caaaggggtg tgccgtggta
     1561 aaggaacgta aaaccgagct ggtcgaggga ttccgccatt cggttcccta tatcaatacc
     1621 caccggggaa aaacgtttgt catcatgctc ggcggtgaag ccattgagca tgagaatttc
     1681 tccagtatcg ttaatgatat cgggttgttg cacagcctcg gcatccgtct ggtggtggtc
     1741 tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc atcaccacga accgctgtat
     1801 cacaagaata tacgtgtgac cgacgccaaa acactggaac tggtgaagca ggctgcggga
     1861 acattgcaac tggatattac tgctcgcctg tcgatgagtc tcaataacac gccgctgcag
     1921 ggcgcgcata tcaacgtcgt cagtggcaat tttattattg cccagccgct gggcgtcgat
     1981 gacggcgtgg attactgcca tagcgggcgt atccggcgga ttgatgaaga cgcgatccgt
     2041 cgtcaactgg acagcggtgc aatagtgcta atggggccgg tcgctgtttc agtcactggc
     2101 gagagcttta atctgacctc ggaagagatt gccactcaac tggccatcaa actgaaagct
     2161 gagaagatga ttggtttttg ctcttcacag ggcgtcacta atgacgacgg tgatattgtc
     2221 tcagaacttt tccctaacga agcgcaagcg cgggtagaag cccaggaaga gaaaggcgat
     2281 tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg
     2341 cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc tgttgcaaga gttgttctca
     2401 cgcgacggta tcggtacgca gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca
     2461 atcaacgata ttggcggtat tctggagttg attcgcccgc tggagcagca aggtattctg
     2521 gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca aattcaccat tattcagctc
     2581 gataacacga ctattgcctg cgccgcgctc tatccgttcc cggaagagaa gattggggaa
     2641 atggcctgtg tggcagttca cccggattac cgcagttcat cacggggcga ggttctgctg
     2701 gaacgcattg ccgctcaggc gaagcagagc ggcttaagca aattgtttgt gctgaccacg
     2761 cgcagtattc actggttcca ggaacgtgga tttaccccgg tggatattga tttactgccc
     2821 gagagcaaaa agcagttgta caactaccag cgtaaatcca aagtgttgat ggcggattta
     2881 gggtaagcga tgaaaatcgc cggatgcggc aggcgtaaca ctcgtgcgtc gcatccggca
     2941 attacgttta tccccgcgaa ctaaacaacg ccgccagacc actgcgccgc tcagtacgag
     3001 tggcgattgc cgcacttaat atacgctcat cggcatacag cgacagacgg cgacgcgcgc
     3061 gggtcaccgc ggtgtatacc agctctcgcg ttactatcgg cgtgcgttgg ctcggtaaaa
     3121 tcaacgccgc atggtcgaac tccgatccct gcgatttatg taccgtcatc gcccacgtag
     3181 tttcgtgctc tggcaagcga ctcggttgca cagacttaat attgccgtcc ggcatcgcaa
     3241 accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca ttaaacaacc
     3301 caagcgcgct gtcattacgg gcaatcatca ccggtcgacc ttcgtaccaa cgagagtgcg
     3361 aatgacgatg aattttgcgc ttctgttgca taaactgctc aattcgctca ttcagtccag
     3421 ccacgccaaa tggcccttcc cgcagggcgc acaaaagttg gtactcattg aacgcctgaa
     3481 tgattaaatc cggctcggca cgcgcttgca gcagatccag ataacgtcca taacccgcaa
     3541 gagcttcctc aagcatcgca atataatctt cgccgctctg taaaagccgt ttttcaatat
     3601 cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt atcaccacgg ttgatcgccg
     3661 cagctaactg accaatgcca gaatcgctgc cgaaacgata gcttttttgc agcaggcaga
     3721 gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc cggaacgtga gttcccgtca
     3781 ggcggcttag ctgcccggca cgctcggcgg taaagccggc attggcataa gcgcagatat
     3841 cgcccagcac agccccagcc tcaaccgagg ccagttgatc acgatcgccg agaaagatca
     3901 ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat cataggcaga tcgatcattg
     3961 acgcttcatc taccaccagc acatcaagat gcagcgggtt accggcatga tgacgtaaac
     4021 gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt gctggcatct tccggaatgc
     4081 gtctcttttg ttcatcggtc agcggtaact gtcgcaaagc cttgccgaga gattcggtta
     4141 agcgcgcggc agctttaccc gttggtgcag ccagacggat acggcagcgt tcgccgtcgg
     4201 ccatttgaat taacgctgcc agcaacttcg ccacggtggt cgttttaccg gtgcctgggc
     4261 cgccggaaat caccgagatc cgccgcgtca gcgccactgc tgccgccact ttttgccagt
     4321 taatttcatc gcttactgga aaaagtttgt ccagggtttg cgccagtaga gcttcatcaa
     4381 cctcaatggc atgattcacc tcattgaaga agcgcgctac cgtccgctcg ttacaccaca
     4441 tgcgattcaa ataaagacga tcgccacaga ggatcatcgg cgtgggttca tctcccctgc
     4501 tgaccgcttg agaagccagc aaggattctt cccaattttg tagctcaccg atttcactga
     4561 cacaggtcgc caacagcgga tgcgacgcct cgttattttc cagtcgtgaa agcggcaaac
     4621 agacgtgtcc ctctccggca tcatgactta acagtgccgc cgcgagggtg acggcaggat
     4681 gttcatctcc cgccacggtc agggcaaatt gcacatccag cgggcgtagc tgtttgtgct
     4741 ccacagcttc cagtaattgc ttttgcaatt tcattacgcc tcctccaggg tcataccggc
     4801 aaacatctca tccatcaggg caatcaaccc ggcgttgggt cgggttgtgt aaatcccttg
     4861 ttgcggatgt tctttatcaa cgccacgcag gaacagatag ataacgccgc caaagtgacg
     4921 atcatagtcg taatcagcaa tgcgatggcg cagatagcga tgcagcgcca gggtataaag
     4981 ctgatattgc agatcatagc ggtgtgcctg catcgctgct gccatagcct gttgggtgta
     5041 agccgaactg tcttcaccca gccagttgga tttatagtcg agcaggtaat aacgcccttc
     5101 gtggcggaat accaggtcga taaagccttt taacatgccg cgtacctgca tgaactccag
     5161 cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt agtgcatcaa gctggctggc
     5221 gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc acctgtttat cgcggtcgga
     5281 aagctgactc aggctaacac ccgtttcatt gagaggtgcc tggaggacag ccgtgatcca
     5341 ctcggtcaac accggttccc actgcgattc aaagccgccg agttccagtt tttcctgtac
     5401 ccagttcggg tcaaccggct gggtaaaatc gaggtcttca aacaaactgt gcaagaatgt
     5461 ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac gtcggttctt caacgacgct
     5521 gaccacgcct gcggcatcaa catccagccg tggcatcaga tcctgagcga taccgtgacc
     5581 acgctgctgc aagccggagt agctggtgac acgccagtta tcgccgggca atcgttgtag
     5641 tgtcctcgca ttcagttctg cagttaaagc atcattaacc tgccagggct ggttatcacc
     5701 agtttgtgcc gtttgccagg caatatcatc atcgcataac gcttcaatac aggtgcgaag
     5761 ccctgccgca tcttgcggtt cccctttttg cagcaaacgc ccgagcgcac tttggtggac
     5821 gtcggtgtca ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc cgagactgca
     5881 atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc agatcttccg ccagacgttc
     5941 gacctccgcg aggtcgacgc tttctggcgc agcattaaga tccaaaactg cctcaaacga
     6001 gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa ttggtaataa atggcaacca
     6061 gaccagcgga tattccagcc ctttcgattt atggatcgtg acaatctgca ccagatgttt
     6121 atcactttcg aggcgcattt gttggctgga ggcattactg tctggctcga ggatatgttg
     6181 cgataaccag cgtaccagcg catgttcact ttccagctgc gttccggctt cttgaagcag
     6241 ttcgctgata tgcaagatat cggtaagacg ccgctcaccg cctgccgttg ccagcaagtt
     6301 ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc ggcataacgc cacgtttgcg
     6361 ccagatttgc cgataaccat cgaactcttc gactaccgca tcccacgcat gttcgtcatt
     6421 attcagcgtt tcaatatcca gcgcattcag ccccatcatt gacgttgcca gcgcactgcg
     6481 cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac agccaaagca tttcctgcgc
     6541 ttccagagtt tcaaaaacac tatcgcggtt cgaaaggtaa acggaaggga tttccagcaa
     6601 cgttaaggca tcgcgcacct gggcggcctc ctggcggctg cgcaccagca cgctgatgtc
     6661 cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc cccgctgtcc
     6721 ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc gccatggtac tttgatagtc
     6781 gccaacgccg cagctttcgc cttccatcag ccacattttc atcgcaggct gcgtttcacc
     6841 tttaaagaca aaacgtaacg cctgattttt cccggctgat ttcactggaa taaacggtat
     6901 ttcgcgaaac atgaacgcgt catcagtctg gctgaaaagc ttgttcacac tgttcaccat
     6961 tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg gcgtgaactt cactacgcgc
     7021 cttcatataa gtgaagatat ccgcaccccg gaatgcatat atggcctgct tcgggtcgcc
     7081 aatcagcaac aatgcggttt ctggctgatg gtgccagata cggcgaaaaa ttcggtactg
     7141 ctgggggtcg gtatcctgaa attcatcgat cattgccacc gggaatcgcg tacggatcgc
     7201 cgctgccaac acctcgccgc tttcgctacg cagcgcggaa tcgagccgac ttaacatgtc
     7261 atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct actgtttcgc ggatctcagc
     7321 caatgcgcgg gtgatcacca gatcacggat cgacaatggt tctgcaagca gttgatcgat
     7381 cgcctcaaac agtggatgtc gcggggtttc ccccccggct ttcgtgcgat cttctaagaa
     7441 acgctgggag aatttttcca gcgactccgg caactgataa ctgtttgtct cttcttctgc
     7501 ccaggcgctg atcttgtcga tccatttagc ctgattgcta cggttaaact tgcgtcgatc
     7561 aataccagaa gattcgatca gcgcatccag ttcacccact gcgtcgcgcc actgctgttt
     7621 taccgtatca atacgcgcca caatttgcgc gtgacgggaa gctagcgttt catcatcggg
     7681 cggcggtgct ttgataaccg gcgcttcgcc ttgcagataa cgattaatat cgcgcagcaa
     7741 cgcctgcggc cctttccagg tttcaaagac gacctgggct atttcacgcg gcagcgggta
     7801 gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt agcagagact catcttcaat
     7861 cagctgctgc tcaaacagca tgccggattc aaaggcattc aggttgagca tgcgctggca
     7921 aaagccgtga atagtaaaga ctgccgcttc atccatctgc cgttcggcta acaacaacca
     7981 ctgcgcggct tgcgctttat cgtcgacctc ttccagcagg tgttcgtaca atggattgtc
     8041 ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata ttgctacgga tacgaccgcg
     8101 caattctgcc gtggcagcct cggtaaaggt gaccaccagc agttcttcaa cggtcagcgg
     8161 gcggggaaag gcggcggaac cgcctagtcc aagtaacagg cgcaaataaa gcgccgcaat
     8221 cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg cgctcgccct gtaagggcaa
     8281 gcgcaaagga tctagtgtct cggcgacatc actcattctt ttcactcatc aggggcattg
     8341 tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcaggatgc acatattcgg
     8401 ctttcccgtt ttggctgccg gaaatctgcg acaggatagc catgccttgc ggctcgacta
     8461 ccgcctgatg gaagaaatcg gccagttttt gcggcgtcag cagtttaatc tgggccacga
     8521 ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt aacttcgatg
     8581 cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc tgctggattt
     8641 gcgcaaactc atctggcttc atcgctcgca attttgcctc tgcggttggg aaaaacgcct
     8701 tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc
     8761 cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaat tgttcttcgg
     8821 tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag ctataggctg
     8881 agctggtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg gagtcggtgc
     8941 tgttaccggc tttttcaaag atgacggatt gttttttatc gaccactaca tctttgtttc
     9001 gacaccactc tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc agcgttgttg
     9061 cctgggcctc ggtcatgttg ccgataacca taaactctgg tcgagcccct gattttaagg
     9121 cgtcgcgata agccagcacc tctttcaacg taatggaggg caaaatttta cgccgttcat
     9181 ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc tgctcaaacg
     9241 ctttaccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc tgctcaagct
     9301 gatcttccgt agcggtatag ctaaagtacc cctcgagcaa tgcctggaac agctgcggca
     9361 gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg gtggaaaaac
     9421 ttatgccacc aaccgacgcc tggttgctta actgatcaag cgccagccct gcgagataat
     9481 cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa
     9541 tcaggctgac atcagctttg ggttcgctgg aaaaataacg gcttggcgca tacaccacgc
     9601 gcagattcga ctcatcaaca atcagctctg gatggtcata tttcttctct gacttaatca
     9661 gcgagaaatc gtcaggaata taggggttaa gctccggtaa ggagagcgca atattggcag
     9721 ctttttgctg ccagtcagcg aaagtttgtt cgctgatttt atcgacctga tacggcgcat
     9781 cgacaaaata agccgtttta ttgtgcggct ctttcgggct gatataccag atacgcgcat
     9841 tctgcggcgt catcatcgcc agacgttctt ttactgcttt agcatcgtac cgatcggcaa
     9901 tattgactgc atccagcgta tgctcaaccg gaacgcgaat catggtatct gccagccatt
     9961 cgacgtaatc catatcacgg gtgatcgacg gataacggaa gtcgatatcc agcacattcg
    10021 ccagttcatc gaagtattgt ttatcaatgc ctttttcacg taacagattg agatagctaa
    10081 aaattgccgc cacaacctga tcgcgattag ccaggccttt atcggttaaa gacgcagaga
    10141 tcgctaatac gccgctgttg ccgttgacga taggatcgga gttggcgcta atgccctcaa
    10201 ctaatccctg cttttgcagc cagtcggaaa gtgtacctgg gctgcgatta ccaatcagat
    10261 aggtaatcaa ttcatccgtt ttactacgga acttcgctga gttgttatcg atgcgaaact
    10321 caacgcgcag aactttacgc ggcattgccg ggacgtaatg aatgataatg cccttttgcg
    10381 cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc ggcacgcgac
    10441 caaaggtgtc cgccgccatt tttgccaact ccggcagcgg tttattgctg taaataaccg
    10501 ccttcatcag attggcggaa tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg
    10561 gattaccagg tttatcgctt aaagtttcga ggttaccacc agaaaacttt gaaccggggt
    10621 gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca cgcgtacgcg
    10681 ccatggttaa ttcagcgttc accgcattac gctcacgttc ggcatatttc ttgtcgagca
    10741 aaggttcagc aatagcatcg gccaggcggt ctaccgcacc aggcaaggcg tcgttctcaa
    10801 cttccagata gaaagccgtg cgatacggcg cagtgctggc attgtgacta ccgccgtgca
    10861 ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc atcagactca
    10921 tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc gacccaacgg
    10981 gcaccaccag cgccgagagc gatttaactg cctgcggatc agaaaccagc aagaccacca
    11041 taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt ttacggatgg
    11101 tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa agggcaacta
    11161 acaacaataa tgctttgaac caggtgctgc ggggcattct cggacctcat aatcaactta
    11221 attttctgtc cagattcaac acgttaacgc aaatcatctg ccagaattta atcttgtgct
    11281 gcacgagtca gcctatgttt atataaccat cagtccgtga ctggtgcgca tcataaagta
    11341 agcggataga ttgcgcaatt tttatacagc actcatgact gattaaagcg aaacagcggt
    11401 aacaggaaac gttgcgactg ttcaacgatg gcctccattg tctctggtgt taattgccgc
    11461 cagagccttt gataccagat atcatcacct tcgccacgca ccatcatgtt accttcgtaa
    11521 gcctgaagga atttcgtacg ggctttttgc aacgtggaat cgtcatccag catggcatcg
    11581 ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa caccagcaat
    11641 ggcgcggaca tcccttcacg atacccctca atcagttgtg agaggtaatg caaagcctgt
    11701 tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc gtagaaaaag gcgactttca
    11761 ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat tccctgcgcc
    11821 acacttaata aagagggacg ccagcgcaac aggccatccg gctgcacctg cggcaaccag
    11881 ccagttatct gcacaccgtt gcaggcgaga tcaatttcca tactctgccc cggctggcga
    11941 caggcaatga ctctgtcggc aagctgctgc atctcctggc actgagtttc ccagaaaatt
    12001 tcaccaaaag cgccatacgg taaatcccct gccgcccgga agcgacggaa caagcgttcg
    12061 gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata acggctaagc
    12121 ccttccagaa taaatggctc ggtgtcgggg atttcgctgt cttcagtacg gaagttcacc
    12181 tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc agaatcgttg tagcgtttcc
    12241 agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga atgtgcttta
    12301 ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc gcctggctga
    12361 tagttttgtg gatcaaacgg cattcgggta tggaggcaag taagatgcgc ttttaccctt
    12421 gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatgact ttgcccgatg
    12481 tagtcgatca gttcctgcac cagtaccgac gggaaacgtt cactgttatc ctgaatggaa
    12541 cgcccgatat agctgatata gagtttttgc tgcgcggaaa ttaacgcttc caggaacaga
    12601 tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg gtttctggct catcaggtca
    12661 aagcccaatg gcgcaagctg acgtggataa acgccgtcgt tcattcccag caggcaaacc
    12721 actttgaacg gaattgaacg cattggcatc agagtacaaa tgttaaccgg tccggcgaga
    12781 aaacgctggc tgatacgttc ttgatccaga cgctgtgcca gttcatcacg caatagtgac
    12841 agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc ctgccattgt
    12901 tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagaa ggcgctgagc
    12961 atatcgcgac aaaccggcaa ccactcttct agcggacgct cctgtgccag cccgcgacgc
    13021 cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc aattaagccg
    13081 ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc catcgcgtag
    13141 cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc ggggagttcc
    13201 agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc cggattcgtt gacccactgg
    13261 cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac cggcacatcc
    13321 agtaatgcca gcacgtcttc cgacacaaag cggctgtctg gcaacgataa caggctgata
    13381 aacgcttcca gtaccggatg cgactgccgc gcccgacggt cggaaatggc gtaaggcagg
    13441 taacgatccg caggtgcgct accaaacaca gcccgaataa acggactgta gctgtcgata
    13501 tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg ggtcttcttc cagcatcgcc
    13561 agcaggcgat cgtgcaaaac ttcaacttca cgctgcgggc tatggcaaac gtggaaggtg
    13621 atactgctat ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa ctcttcgatg
    13681 ttcacaccag caacggcacg gttttccagt tccagaatgt cagactgaat gttatgcagc
    13741 aggttatctg gcgtgacatc gacaaaggcg tccagctcct ggctgctctc caggtcagaa
    13801 aggagataaa tgtagtcgcg cccaagctta ccccatgaag ccagcagcgg gttgccgaca
    13861 tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctatcacg aaatagcggc
    13921 aattcgcgat cttcaaaact gtgtcgacgc tgacgggtca gcagtttcgc cagataagca
    13981 ggatctttaa tatcgcccca gtaataacgg caggggttgg taaacaggag atggatttca
    14041 atatgtttac ccagcgcctg tagcgcctgg agataaacag gcggtaacgc ggaaataccg
    14101 catataaaga cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc cagcgtttcg
    14161 ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gcccgagttg atgggtatat
    14221 tccaccagcg ccttccacaa cggggcttgc caggcctgtg cttctcccag cccttcaacc
    14281 aaatgtcctg tttcccactg tgccagccag tccggacgat agaccagata ctggtcaaac
    14341 aggtccgccg cttttgagga aagctggaac agttttcgct tgtcgctatc gtcagtcaga
    14401 taatgccgca acagggtaaa gtcttcgcgc tccagcaatt gcggcagcag agtcatcagt
    14461 ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatttccgg taacacccgg
    14521 acgaacatat cccagataaa gctcgctggc agcggaaaat caatgtttgc cgcaatacta
    14581 aacttttgcg acagggtcat ttgcagccac tgcgccatac cggtactttg caccagaatc
    14641 atctctggtt cgaaaggatc gtccagccgt tcgcgttcga caataaactc catcaacgct
    14701 tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc tgactactga
    14761 cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga aactagcgta
    14821 acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg ccagttggca
    14881 ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat gccgccagag ctgttggtac
    14941 tggtttctgc tggcgagact gttcattaat gttcgctgga tacccgataa cgcagtgaca
    15001 atcatcacca tcagcaccat cgcaaccatt acctccggca ggctaaagcc ttgttgattc
    15061 ttcagggaag ctgacataac gccctctctt tcagcggaca aaaatcgctc cagccgcgtg
    15121 gcgaaaaaac aatgtttcca tcaatgactt cgcctgttcg ccacaacgaa acgccttcat
    15181 aaccggcaac caataaggct tcattatctg ccagtaaacg caaacaaacc cgggcatcgg
    15241 ttccagcgta ctgcgagcac tgaactgcgg gctgcgtctg ccaggagtgc attttccccc
    15301 acgccagcgc cgactgaacg atggcctggc ggcgcaatga ctgactttct atgctcacgc
    15361 gagaggcaaa actgcgatcc tgctgactca ttccttgcaa tagcaagcta cccaaaacca
    15421 gcagcatcag gaccagagcc agtgacgaaa cacctttttc gcggttcaca ggttgaatcc
    15481 tgtcacgcta tagctggcat ccaccacggt ttgcggttca gacttactgg cagcacgcat
    15541 attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct gaaaagtgtc
    15601 gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac aggatgtcgc
    15661 accgcgtagc gtttccagca catgctcctt cagacgaaat ccaatctggt cagactcttt
    15721 taccggttcg cgatcccaga taccgttact gttcgcatcc cactgcacaa tgatacagtc
    15781 accctgtccg acaatttcca gcccttcgcc ggtacagctg ccatgacaat aacccgccct
    15841 ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca gcgccagctt
    15901 acgggtgctc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgcag cccccagcaa
    15961 caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac cttgctcttt
    16021 tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca cggcgaaacc
    16081 accagccacc actcgcccgt tgagtttttg aagcgaatat gcccggccca tgcggtattg
    16141 cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc gggccagcgt
    16201 ggcacaaaga ccaatggtga actgccatga caggtattgg ccccagcagc ggaactcaca
    16261 aggcaccata acgtcccctc cctgataacg ctgatactgt ggtcgcggtt atgccagttg
    16321 gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg ccatagccgt
    16381 tgcgactgct gccagtattg ccagccatag agtccacttg cgcttagcat gaccaaaatc
    16441 agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat gccggcagta
    16501 tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcgca tcttccggac
    16561 tatttacgcc gttgcaggac gttgcaaaat ttcgggaagg cgtctcgaag aatttaacgg
    16621 agggtaaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc gtaattagat
    16681 agccaccggc gctttaatgc ccggatgtgg atcgtagcct tcaatctcaa agtcttcgaa
    16741 acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca gcggacgcgg
    16801 ttcgcggctt aattgcagat gagtttgatc catatgattg ctgtacaggt gcgtatcgcc
    16861 accggtccag acaaaatcac ccacttccag atcgcactgc tgcgccatca tatgcaccag
    16921 taacgcgtag ctggcaatgt tgaacggcag gccgaggaag acgtcacagg agcgctgata
    16981 aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaatgcat ggcacggtgc
    17041 cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc ggcgggaatc
    17101 cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg tcaatatgac gaccatctgg
    17161 cgttggccag gcgcgccact gtttaccata cactggtccg aggtcgccgt tttcatcggc
    17221 ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag tgtcgccctg
    17281 cagaaaccac agcagttcgt ggatgatgga acgcaggtgg caacgtttag ttgtcaccag
    17341 cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa gcgttccggt
    17401 tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca gttctaaata
    17461 ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg cccagaccat
    17521 catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgccccatgc tgatgtactg
    17581 cacccaggca ccggtaaact gcgcgtcggg ctggcggaaa aactcaacaa tgatgcgaaa
    17641 cgcgccgtag ccaatcagga acaagccaga gacagctccc attgggcgcg gtttacgaat
    17701 atacaggttg aggataataa acagcaccac accttccagc agcagctcgt aaagctgtga
    17761 tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat tgccactgcg ggttggtttg
    17821 cagcagcaaa atatcttctg tacgggagcc tgggaacagc atggcaaacg ggaagttcgg
    17881 gtcaacgcgg ccccacaatt caccgttaat aaagttgccc agacgcccgg caccaagacc
    17941 aaacggaatg agtggtgcga taaaatcaga aacctggaag aaggaacgtt tagtacggcg
    18001 ggcaaagata atcatcacca cgataacgcc aatcaggccg ccgtggaaag acatgccgcc
    18061 gtcccagaca cggaacagat acagcggatc ggccataaac tgcgggaaat tgtagaacag
    18121 aacataacca atacgtcccc cgaggaagac gccgaggaag cccgcataga gtaagttttc
    18181 aacttcattt ttggtccagc cgctgcccgg acgattcgcc cgtcgtgttg ccagccacat
    18241 tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca cgggtcctat
    18301 tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca tctgtcacca
    18361 caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcgcgccg caggtttcag
    18421 gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg ataccgttaa
    18481 aagatatgta tatgatccgc gctataaccc tccgcgaatc agcccgccca tgccgcgacg
    18541 ctccataaag gctgcaacct gatggcgaac ttcggtcgcc agttgcgctt ccagactacg
    18601 ctgcgcaaga ttttctgctt cggcataatc aatgcgccgc agcaggtatt ttacccgcgc
    18661 tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga ttgccacgca
    18721 catgggatcg cccgccattt caccgcacaa acggagatcg attccatgta tttccgcttc
    18781 ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcat aaatgttcgc
    18841 cacccgggta ttgttgcgat caacggccag aatgtattga gtcagatcgt tggtgccaac
    18901 agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg acggcacttc
    18961 cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct cgacttcacg
    19021 tccggcacgt tcaatcaggc ggcgtgcttc atcaacttca tcgaggcttg tgaccatcgg
    19081 caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc gcacctggat
    19141 caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca ggcatggatt
    19201 ctcttcgctg atcggcatgt aaggcagctg cttatctgct ccgacatcca gcgtacgcaa
    19261 ggtgacgggt ttatcattga acatttgcag catcccctga tactgcgcca cctgttcttc
    19321 ttccgacggg aaaccacttt gcagcatgaa tgggatttca gtgcgataaa gaccgatgcc
    19381 atcaatacgg ctgcccagtt tttcttcatg ttccgggctt aaaccagcat tgagcatgac
    19441 ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg acgtcatctt ccgccagacg
    19501 gctaagctca atctcttcac taattagccg ctgatattct tgcagcagta ccggctccgg
    19561 atcgaccagc aattcaccgc gatagccatc aacgatcagc gtcctacgat gcagcaccga
    19621 aggctgaata tccgcgccca tcacggtagg gatccccagc gcacgtacca tgatcgcagc
    19681 atgggagttg gctgcgccat ctcgcacgac aacaccgact aagcgatcct ggggcagctc
    19741 agcaagagtt gtcgctgaca gttcatctgc caccagaatg aaacgttccg gccaggcgtt
    19801 cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg cacgtaaatc
    19861 gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg caaatttttc
    19921 aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg caaacaattc
    19981 gcgacgcagc cgggtgtccg aaagtaggtg cgagtaaaga tcgaaaatag ccgccgtttc
    20041 tttttgtgct ccggcggcaa agcgtttgct gtagcggcga aactcgtttg ccgcttcttc
    20101 cagcgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg cctgatacac
    20161 ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca caccaggtgc
    20221 tgccggtaat gcgcggattc gcgtctggcg atattgccca aacaaggcag tcaactgcga
    20281 ctgagaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt cttcactttc
    20341 gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact ggcgacgttg
    20401 aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg ggatgtattt
    20461 gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc tgccaaccag
    20521 gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt ttttcagccc
    20581 ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga ccgaacagac
    20641 ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct cattcaggcg
    20701 tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa ttggcgtgac
    20761 ttaacctctt ttacgtcgat aagcagatgt gttttgtggt tttggcgtat tttcctgcag
    20821 tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac gtttaaatga
    20881 caccacctgt ctgaccggat accagtaact tacccatcgc cagccgtcaa actctggtgt
    20941 actgctggtt tgcatattga tttctgcatc gccgctcacc agctgcaaga gaaaccattt
    21001 ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt tcggtaattt
    21061 gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc ttaatcctac
    21121 ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt tgattccgcc
    21181 ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct gcccctggcg
    21241 attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca ccggactacc
    21301 tcaaaataaa gctttatata cgaatgattg tttcatactc caagaagacg gtaaaccact
    21361 ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt gcagagttat
    21421 aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata gagttgtgaa
    21481 gaactacgga attactacgg gaaaacccgg agcattctga ataagcccga tatagacatt
    21541 ttaaaatatt cattattagt caatgcatta catgtaattt gagtaatgaa aaagagtaat
    21601 tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa agatcaacca atgccgtatt
    21661 tatccacaga atgtgccact aagttaagca ctgaaccact aaaaactgga gtttcgtcgc
    21721 acgtcaaggc tgtaaatgga aacagtagtg gaggtttttc acagttatcc cagctttctg
    21781 tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa aacctattgc
    21841 agtgttgcgc aactcgatta ccggcagcct aaaaagcaat ataaatcaga gaattgaaca
    21901 acacatgtgg aaaaagttac actgcgaata ttcggcacat aattgctgtt tgttttttaa
    21961 ccaaggtatc atgacatgtc ccaacctcgc ccactcctct ctcctcccga aactgaagaa
    22021 cagttgttag cgcaagcaca gcaactttct ggttatacat tgggagaact ggcggcactt
    22081 gccgggctgg ttacgccaga gaatttaaaa cgcgataagg gctggattgg cgtgttactg
    22141 gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc tgctctgggc
    22201 gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac aacattcgtt
    22261 tgtgttgccc cgttaacggg taatagcggg gtgacctggg aaaccagcca tgtgcgccac
    22321 aagctcaaac gcgtactgtg gataccggtt gaaggtgagc gcagcatccc gctggcgcag
    22381 cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg acagctacgc
    22441 gaagactggg aagaattaat ggatatgatt gttctcggtc aggttgagcg gatcaccgct
    22501 cgtcacgggg agtatttaca gatacgaccg aaagcagcga atgcgaaagc gcttaccgaa
    22561 gccattggtg cccggggcga acggattctg acgctgccac gcggctttta tttgaagaag
    22621 aatttcacca gtgcactact ggcccgtcat tttctgatcc agtagccatc gctttgacct
    22681 gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt tagatgttat
    22741 ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg tacgctgacg ctgctggaga
    22801 tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag ctccccacag
    22861 cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggttatg cgtctggcgc
    22921 tgctggcatc aatcgcctgg gttacgcgcc tgacaaatcc gctttttaca atattcagtc
    22981 aggaaatttc cgcccgtgat ttgattctgc ttctgggtgg cttgttcctt atctggaaag
    23041 ccagcaagga aatccacgaa tccattgaag gtgaagaaga agggctgaaa acacgcgttt
    23101 catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttc agcctcgact
    23161 cggtgattac cgctgtgggt ctgtcagatc acctgtttat tatgatggcc gccgtggtga
    23221 ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc gaacgccatc
    23281 cttcggtaaa aatgctggcg ctctctttcc tgattctggt cggctttacc ctgattctgg
    23341 aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc ttctctattg
    23401 cggttgaaag cctcaacctg attcgcaaca aaaagaaccc gctctgatac tccgtacgct
    23461 ctcctgcggg agggcgtact cactcctgtt accctcacag tttaagattt tcctgctttc
    23521 aagatatata acgtcggttt ataaacagac tattatcata ggtgggccaa atgaggataa
    23581 ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg ctgtttccgg
    23641 gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga ccgatggaaa
    23701 acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag gtaacgcgat
    23761 gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa ggtcagcatc
    23821 cggctgtaat gccgatcagt taag
//
LOCUS       DAEEBB010000057        23691 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEEBB010000057 DAEEBB010000000
VERSION     DAEEBB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.057"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(506..1303)
                     /locus_tag="KLF07_002115"
     CDS             complement(506..1303)
                     /locus_tag="KLF07_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9832127.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="KLF07_002116"
     CDS             complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="KLF07_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBD9832128.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="KLF07_002117"
     CDS             complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="KLF07_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBD9832129.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="KLF07_002118"
     CDS             complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="KLF07_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBD9832130.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3229..4887
                     /gene="fliF"
                     /locus_tag="KLF07_002119"
     CDS             3229..4887
                     /gene="fliF"
                     /locus_tag="KLF07_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBD9832131.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4880..5875
                     /gene="fliG"
                     /locus_tag="KLF07_002120"
     CDS             4880..5875
                     /gene="fliG"
                     /locus_tag="KLF07_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBD9832132.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5868..6554
                     /gene="fliH"
                     /locus_tag="KLF07_002121"
     CDS             5868..6554
                     /gene="fliH"
                     /locus_tag="KLF07_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBD9832133.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6554..7927
                     /gene="fliI"
                     /locus_tag="KLF07_002122"
     CDS             6554..7927
                     /gene="fliI"
                     /locus_tag="KLF07_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBD9832134.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7946..8389
                     /gene="fliJ"
                     /locus_tag="KLF07_002123"
     CDS             7946..8389
                     /gene="fliJ"
                     /locus_tag="KLF07_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBD9832135.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8386..9513
                     /gene="fliK"
                     /locus_tag="KLF07_002124"
     CDS             8386..9513
                     /gene="fliK"
                     /locus_tag="KLF07_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBD9832136.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9618..10082
                     /gene="fliL"
                     /locus_tag="KLF07_002125"
     CDS             9618..10082
                     /gene="fliL"
                     /locus_tag="KLF07_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBD9832137.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            10087..11091
                     /gene="fliM"
                     /locus_tag="KLF07_002126"
     CDS             10087..11091
                     /gene="fliM"
                     /locus_tag="KLF07_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBD9832138.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11088..11501
                     /gene="fliN"
                     /locus_tag="KLF07_002127"
     CDS             11088..11501
                     /gene="fliN"
                     /locus_tag="KLF07_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBD9832139.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11504..11869
                     /gene="fliO"
                     /locus_tag="KLF07_002128"
     CDS             11504..11869
                     /gene="fliO"
                     /locus_tag="KLF07_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBD9832140.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11869..12606
                     /gene="fliP"
                     /locus_tag="KLF07_002129"
     CDS             11869..12606
                     /gene="fliP"
                     /locus_tag="KLF07_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBD9832141.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12616..12885
                     /gene="fliQ"
                     /locus_tag="KLF07_002130"
     CDS             12616..12885
                     /gene="fliQ"
                     /locus_tag="KLF07_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBD9832142.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12894..13679
                     /gene="fliR"
                     /locus_tag="KLF07_002131"
     CDS             12894..13679
                     /gene="fliR"
                     /locus_tag="KLF07_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBD9832143.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13964..14587
                     /gene="rcsA"
                     /locus_tag="KLF07_002132"
     CDS             13964..14587
                     /gene="rcsA"
                     /locus_tag="KLF07_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBD9832144.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="KLF07_002133"
     CDS             complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="KLF07_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBD9832145.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14982..15209
                     /gene="yodD"
                     /locus_tag="KLF07_002134"
     CDS             14982..15209
                     /gene="yodD"
                     /locus_tag="KLF07_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBD9832146.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15507..16322
                     /locus_tag="KLF07_002135"
     CDS             15507..16322
                     /locus_tag="KLF07_002135"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBD9832147.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16319..18013)
                     /gene="yedQ"
                     /locus_tag="KLF07_002136"
     CDS             complement(16319..18013)
                     /gene="yedQ"
                     /locus_tag="KLF07_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBD9832148.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18184..18366)
                     /locus_tag="KLF07_002137"
     CDS             complement(18184..18366)
                     /locus_tag="KLF07_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBD9832149.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18445..19362)
                     /locus_tag="KLF07_002138"
     CDS             complement(18445..19362)
                     /locus_tag="KLF07_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBD9832150.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19535..20456
                     /gene="yedA"
                     /locus_tag="KLF07_002139"
                     /pseudo
     CDS             19535..20456
                     /gene="yedA"
                     /locus_tag="KLF07_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="KLF07_002140"
     CDS             complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="KLF07_002140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBD9832151.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="KLF07_002141"
     CDS             complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="KLF07_002141"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBD9832152.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22381..23037)
                     /locus_tag="KLF07_002142"
     CDS             complement(22381..23037)
                     /locus_tag="KLF07_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBD9832153.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(23077..23442)
                     /locus_tag="KLF07_002143"
     CDS             complement(23077..23442)
                     /locus_tag="KLF07_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD9832154.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5897 a         6137 c         5894 g         5763 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct
     1141 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
     1201 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
     1261 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
     1321 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
     1381 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
     1441 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
     1501 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
     1561 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
     1621 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
     1681 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
     1741 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
     1801 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
     1861 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
     1921 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
     1981 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
     2041 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     2101 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     2161 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     2221 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     2281 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     2341 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     2401 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     2461 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     2521 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     2581 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     2641 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     2701 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     2761 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     2821 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     2881 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     2941 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     3001 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     3061 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     3121 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3181 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3241 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3301 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3361 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3421 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     3481 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3541 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     3601 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3661 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3721 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3781 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     3841 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3901 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3961 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     4021 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     4081 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4141 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4201 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4261 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4321 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4381 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4441 gaagatctga cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4501 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4561 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4621 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4681 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4741 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4801 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4861 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4921 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4981 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     5041 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     5101 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5161 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5221 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5281 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5341 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5401 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5461 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5521 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5581 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     5641 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     5701 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     5761 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     5821 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     5881 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     5941 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     6001 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     6061 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     6121 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     6181 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     6241 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     6301 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     6361 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     6421 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     6481 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     6541 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     6601 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     6661 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     6721 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     6781 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     6841 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     6901 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     6961 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     7021 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     7081 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     7141 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     7201 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     7261 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     7321 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     7381 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     7441 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     7501 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     7561 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     7621 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     7681 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     7741 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     7801 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     7861 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     7921 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     7981 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     8041 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     8101 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     8161 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     8221 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     8281 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     8341 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     8401 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     8461 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     8521 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     8581 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     8641 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     8701 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     8761 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     8821 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     8881 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     8941 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     9001 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     9061 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     9121 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     9181 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     9241 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     9301 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     9361 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     9421 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     9481 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     9541 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     9601 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     9661 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     9721 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     9781 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     9841 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     9901 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     9961 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
    10021 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
    10081 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
    10141 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
    10201 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
    10261 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
    10321 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
    10381 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
    10441 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
    10501 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
    10561 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
    10621 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
    10681 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
    10741 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
    10801 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
    10861 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
    10921 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
    10981 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
    11041 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    11101 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    11161 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    11221 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    11281 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    11341 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    11401 taatctccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    11461 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    11521 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    11581 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    11641 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    11701 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    11761 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    11821 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    11881 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    11941 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    12001 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    12061 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    12121 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    12181 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    12241 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    12301 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    12361 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    12421 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    12481 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    12541 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    12601 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    12661 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    12721 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    12781 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    12841 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    12901 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    12961 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    13021 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    13081 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    13141 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    13201 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    13261 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    13321 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    13381 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    13441 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    13501 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    13561 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    13621 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    13681 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    13741 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    13801 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    13861 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    13921 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    13981 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    14041 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    14101 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    14161 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    14221 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aaattattga taagttctaa
    14281 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    14341 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    14401 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    14461 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    14521 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    14581 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    14641 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    14701 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    14761 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    14821 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    14881 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    14941 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    15001 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    15061 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    15121 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    15181 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    15241 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    15301 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    15361 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    15421 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    15481 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    15541 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    15601 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    15661 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    15721 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    15781 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    15841 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    15901 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    15961 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    16021 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    16081 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    16141 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    16201 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    16261 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    16321 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    16381 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    16441 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    16501 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    16561 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    16621 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    16681 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    16741 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    16801 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    16861 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    16921 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    16981 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    17041 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    17101 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    17161 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    17221 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    17281 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    17341 taaaccagcg taccgcacgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    17401 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    17461 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    17521 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    17581 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    17641 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    17701 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    17761 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    17821 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    17881 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    17941 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    18001 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    18061 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    18121 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    18181 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    18241 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    18301 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    18361 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    18421 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    18481 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    18541 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    18601 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    18661 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    18721 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    18781 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    18841 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    18901 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    18961 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    19021 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    19081 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    19141 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    19201 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    19261 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    19321 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    19381 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    19441 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    19501 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    19561 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    19621 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    19681 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    19741 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    19801 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    19861 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    19921 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    19981 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    20041 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    20101 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    20161 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    20221 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    20281 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    20341 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    20401 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    20461 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    20521 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    20581 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    20641 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    20701 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    20761 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    20821 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    20881 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    20941 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    21001 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    21061 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    21121 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    21181 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    21241 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    21301 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    21361 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    21421 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    21481 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    21541 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    21601 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    21661 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    21721 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    21781 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    21841 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    21901 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    21961 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    22021 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    22081 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    22141 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    22201 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    22261 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    22321 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    22381 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    22441 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    22501 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    22561 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    22621 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    22681 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    22741 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    22801 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    22861 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    22921 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    22981 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    23041 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    23101 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    23161 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    23221 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    23281 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    23341 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    23401 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    23461 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    23521 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    23581 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    23641 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct t
//
LOCUS       DAEEBB010000058        23488 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEEBB010000058 DAEEBB010000000
VERSION     DAEEBB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.058"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="KLF07_002144"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="KLF07_002144"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBD9832155.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="KLF07_002145"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="KLF07_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBD9832156.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="KLF07_002146"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="KLF07_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBD9832157.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="KLF07_002147"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="KLF07_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBD9832158.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="KLF07_002148"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="KLF07_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBD9832159.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="KLF07_002149"
     CDS             5280..5738
                     /locus_tag="KLF07_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBD9832160.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="KLF07_002150"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="KLF07_002150"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBD9832161.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="KLF07_002151"
     tRNA            6790..6874
                     /locus_tag="KLF07_002151"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="KLF07_002152"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="KLF07_002152"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="KLF07_002153"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="KLF07_002153"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBD9832162.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="KLF07_002154"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="KLF07_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBD9832163.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="KLF07_002155"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="KLF07_002155"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD9832164.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="KLF07_002156"
     CDS             complement(10156..13899)
                     /locus_tag="KLF07_002156"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBD9832165.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="KLF07_002157"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="KLF07_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBD9832166.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="KLF07_002158"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="KLF07_002158"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBD9832167.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="KLF07_002159"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="KLF07_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBD9832168.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="KLF07_002160"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="KLF07_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBD9832169.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="KLF07_002161"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="KLF07_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBD9832170.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="KLF07_002162"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="KLF07_002162"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBD9832171.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="KLF07_002163"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="KLF07_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBD9832172.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="KLF07_002164"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="KLF07_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBD9832173.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="KLF07_002165"
     CDS             23294..23401
                     /locus_tag="KLF07_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD9832174.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5885 a         6142 c         5664 g         5797 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cgaatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg caggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtcttt
//
LOCUS       DAEEBB010000059        23235 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEEBB010000059 DAEEBB010000000
VERSION     DAEEBB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.059"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="KLF07_002166"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="KLF07_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBD9832175.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="KLF07_002167"
     CDS             507..1136
                     /locus_tag="KLF07_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBD9832176.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="KLF07_002168"
     CDS             1163..2824
                     /locus_tag="KLF07_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBD9832177.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="KLF07_002169"
     CDS             3063..3122
                     /locus_tag="KLF07_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD9832178.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="KLF07_002170"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="KLF07_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBD9832179.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="KLF07_002171"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="KLF07_002171"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBD9832180.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="KLF07_002172"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="KLF07_002172"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBD9832181.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="KLF07_002173"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="KLF07_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBD9832182.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="KLF07_002174"
     CDS             9505..10125
                     /locus_tag="KLF07_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBD9832183.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="KLF07_002175"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="KLF07_002175"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBD9832184.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="KLF07_002176"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="KLF07_002176"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD9832185.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="KLF07_002177"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="KLF07_002177"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBD9832186.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="KLF07_002178"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="KLF07_002178"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBD9832187.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="KLF07_002179"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="KLF07_002179"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="KLF07_002180"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="KLF07_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBD9832188.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="KLF07_002181"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="KLF07_002181"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBD9832189.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="KLF07_002182"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="KLF07_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBD9832190.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="KLF07_002183"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="KLF07_002183"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBD9832191.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="KLF07_002184"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="KLF07_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBD9832192.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="KLF07_002185"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="KLF07_002185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD9832193.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="KLF07_002186"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="KLF07_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBD9832194.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6331 c         5907 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc gtgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DAEEBB010000060        22767 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEEBB010000060 DAEEBB010000000
VERSION     DAEEBB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.060"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..438
                     /gene="traV"
                     /locus_tag="KLF07_002187"
     CDS             <1..438
                     /gene="traV"
                     /locus_tag="KLF07_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBD9832195.1"
                     /translation="KVTGMRLLLPSSTDELITTATWQVSVPDETPFDVQHVIQWILPS
                     PLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYIT
                     RRFQCFPLASLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            405..1607
                     /gene="traW"
                     /locus_tag="KLF07_002188"
     CDS             405..1607
                     /gene="traW"
                     /locus_tag="KLF07_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBD9832196.1"
                     /translation="MQRKTLLAALIAILSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     DESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            1636..2220
                     /gene="traX"
                     /locus_tag="KLF07_002189"
     CDS             1636..2220
                     /gene="traX"
                     /locus_tag="KLF07_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBD9832197.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQRMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRLCC
                     WSLLAIALFLSGMLFAASSLPLITQVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            2317..4485
                     /locus_tag="KLF07_002190"
     CDS             2317..4485
                     /locus_tag="KLF07_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBD9832198.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGTASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQKAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYR
                     TQLQNSSYTPPLGTVVSSSEQRMANAQDPKSALSEIMGPLVSLTNRIATETSGTGTTS
                     AQINPLIKMKNIGDITMVSAEGIWTIYTTARVVVAGGKDSILVKFFNSLTGAASMLTA
                     LFEALAPPVYFLLFLMFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGRDGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDNTKGSDDKDGIKG"
     gene            4556..5218
                     /gene="excA"
                     /locus_tag="KLF07_002191"
     CDS             4556..5218
                     /gene="excA"
                     /locus_tag="KLF07_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBD9832199.1"
                     /translation="MKAKKETTDRFPTWWLFYYVLRKAYFFLGIPFFLFCALGFTEML
                     CSDRYSGNKVEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKKLEIHTK
                     YINLPMVPVPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAE
                     VF"
     gene            complement(5290..5499)
                     /locus_tag="KLF07_002192"
     CDS             complement(5290..5499)
                     /locus_tag="KLF07_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBD9832200.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            5901..6077
                     /locus_tag="KLF07_002193"
     CDS             5901..6077
                     /locus_tag="KLF07_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832201.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVPGKTRLMFDSK
                     EQALEVKEGYKFLT"
     gene            complement(6142..6438)
                     /locus_tag="KLF07_002194"
     CDS             complement(6142..6438)
                     /locus_tag="KLF07_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832202.1"
                     /translation="MSALRHVLSGILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            6738..6989
                     /locus_tag="KLF07_002195"
     CDS             6738..6989
                     /locus_tag="KLF07_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832203.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRGNETIPDTLH"
     gene            complement(7061..7213)
                     /locus_tag="KLF07_002196"
     CDS             complement(7061..7213)
                     /locus_tag="KLF07_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBD9832204.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            7528..8619
                     /locus_tag="KLF07_002197"
     CDS             7528..8619
                     /locus_tag="KLF07_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein finQ"
                     /protein_id="HBD9832205.1"
                     /translation="MEMSQLKQPIFLKKIKKVINTIPGLEEQIFACRNKKRSDNPLLF
                     IDRKDEERILMSRLQSQQKNEELASKLESLFHGNELSSPHSILCFIYWRYTKKIYRLS
                     EDIISDVANTYVDNIPAQTLKELPSWSIYVSAENLHTILPTSYPIHGFFFYPFLDKNG
                     NIIRLFIIDDLKQSQGTTGLKEKNVDVVNNIIRIKDSREGLLDSRKMECIDGEVVVTV
                     NEKLKDFRDREFNLLNAQISMVLYICSQINDIKEKNQFKRSEKHKKHVHTHHELPAQN
                     IREWDVGIRMGQAIRQYRQAEPTGKERTTIGSKRPHIRRGHWHTYWTGSKKPELAHER
                     KPRLIWLPPVPVNLEDVNKLPVVITPIDK"
     gene            8927..10135
                     /gene="trbA"
                     /locus_tag="KLF07_002198"
     CDS             8927..10135
                     /gene="trbA"
                     /locus_tag="KLF07_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBD9832206.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLEDGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVTAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFKRVIKSDGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            10154..11224
                     /gene="trbB"
                     /locus_tag="KLF07_002199"
     CDS             10154..11224
                     /gene="trbB"
                     /locus_tag="KLF07_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBD9832207.1"
                     /translation="MTIEYFATRIAKHISVQAIWPGGRTEIIAVLPQDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRAII
                     ASVVLFVMAMIAVSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VTPKKDVQVPEKTLTSKNPEAAAARHNLAAVLKRNADRGMFTVNLSSGHERTLYAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            11217..13508
                     /locus_tag="KLF07_002200"
     CDS             11217..13508
                     /locus_tag="KLF07_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBD9832208.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRIRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMARLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRIRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTQEERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNTP
                     PTERPVNGFHHKKTNRPDWDGMY"
     gene            complement(13580..14398)
                     /locus_tag="KLF07_002201"
     CDS             complement(13580..14398)
                     /locus_tag="KLF07_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HBD9832209.1"
                     /translation="MSTNLAFRYASVETFFKMIENQELWFTDLRNMNDPEEYSAGYEI
                     VKRIINEEFPDCIRLLEEVSPNKIDENCKILICCFCSDGDRSNMWSEYGDNGKGVAIG
                     YDKNIITQYHILARFEEHFQPISGRVVFHPVIYSEELFREYVREALHDYIKRSSLNIE
                     YAHGILHAYLIRLCSLYKRESFECENEVRGLIEVDKHKSPPYKLEERSTDYGIAYYTK
                     VKFACNNITSIREIVLGPLFEQSDEQLRSKLDDLGLNNIKIRRSICTCNDAVNL"
     gene            complement(14520..17222)
                     /gene="nikB"
                     /locus_tag="KLF07_002202"
     CDS             complement(14520..17222)
                     /gene="nikB"
                     /locus_tag="KLF07_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBD9832210.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PYRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWVRV
                     QHGHLVICDGYEHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKLWEPPSPMR"
     gene            complement(17233..17565)
                     /locus_tag="KLF07_002203"
     CDS             complement(17233..17565)
                     /locus_tag="KLF07_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBD9832211.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            17798..18133
                     /locus_tag="KLF07_002204"
     CDS             17798..18133
                     /locus_tag="KLF07_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBD9832212.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(18219..19067)
                     /locus_tag="KLF07_002205"
     CDS             complement(18219..19067)
                     /locus_tag="KLF07_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBD9832213.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            19646..19897
                     /locus_tag="KLF07_002206"
     CDS             19646..19897
                     /locus_tag="KLF07_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9832214.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(19928..20134)
                     /locus_tag="KLF07_002207"
     CDS             complement(19928..20134)
                     /locus_tag="KLF07_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832215.1"
                     /translation="MTIAERLIQKGFDEGFDEGFKEGFKKGALEVAREAACRLRDMGW
                     TPERIQEAAGLSGEELKKLFPDEQ"
     gene            complement(20196..21110)
                     /locus_tag="KLF07_002208"
     CDS             complement(20196..21110)
                     /locus_tag="KLF07_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBD9832216.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGSKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKIIH"
     gene            complement(21107..21496)
                     /locus_tag="KLF07_002209"
     CDS             complement(21107..21496)
                     /locus_tag="KLF07_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HBD9832217.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(21673..21939)
                     /locus_tag="KLF07_002210"
     CDS             complement(21673..21939)
                     /locus_tag="KLF07_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832218.1"
                     /translation="MKPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(22033..22467)
                     /locus_tag="KLF07_002211"
     CDS             complement(22033..22467)
                     /locus_tag="KLF07_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBD9832219.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
BASE COUNT         6113 a         5740 c         5263 g         5651 t
ORIGIN      
        1 aaggttacag gcatgcggct gttgttgccg tccagcacag atgagctgat aacaacggca
       61 acatggcagg tttctgtccc ggatgaaaca ccattcgacg ttcagcatgt catccagtgg
      121 atactccctt caccgctggc ttcgctgatg tccgatgata cctggttatg gccatccgtt
      181 cccggaacgc cggcactgag tgccggtgac tggcctgtta tggatactga cccttccacc
      241 ttacggcgcg tcagacgtcg ttctttcagc atcatcgata accggaacag gaaaggttat
      301 atcaccaggc gatttcagtg tttccctctt gcatcgctac ctgaagacag aaaatacgac
      361 agccttcctg accttgtgct ccatcccgag caggagacaa taaaatgcaa agaaaaacat
      421 tactcgcagc ccttatagcc atcctgagtg gaacggcatg ccaggctcat gcttattcag
      481 tcaccgtcgt cgccagccgg cctgttgaag agcaggtcat ccccagaatg gaagccatca
      541 aagatgtcct tggcaacata ctgagtaccc agacagccac gggtaccgct atcaatcaga
      601 actctgaaaa gctggcaagt gtcattgccc agaacggaca ggcgacacgt cagcagatga
      661 ttttcagtaa tgaaactcag cgcctcgagg aggcacggaa aagcttcacc gtgccagact
      721 ccatctgcag tgaatccgca tcagggattg caacagaaag caaaagtgcc tcagcatccg
      781 cagcctcaaa actcagtaaa ggtggcggtg tgagcaaccg tagcatccgc gaccgtctgg
      841 ccagcgcagc aaactctccc gtacgggaag cttatgatgg cgcggccata cacgccagtt
      901 actgtacaga agcagaatat gcacgttttg gaggaaccgc cgtttgtcct tctgtgggcg
      961 aaataccagg tggagacagt caggtacgct cgatttacca cggcgcaggt acagcggaca
     1021 cacctgcggc actgacatgg gatcagaagc aaatagatgc agcgacggct tacatgaaaa
     1081 acacttcccg cccctctgcc ggtcgtgctc tgggtaaagg agaagtgaat acccagagtg
     1141 ggcgaaccta cgtcggactc cagaacgaat acaacggcat cattgattct gcgtcgaatc
     1201 cccaactgac gctgattgct gacagcacgc ctgatgaaag cacacgtaaa gcactggctg
     1261 aaacactgca gtcagactca gcggccgctt acttcgacca ggtggcttca ccagaagcaa
     1321 aagcacgggg atatatgagt acccgtgagt ttgaagcttt tgaggctggc cggcgctacg
     1381 ccaacacagc atacctggtg gatttacagg aaatgcaggg ggataacctg ctccgtgaac
     1441 tggtaagaat cactgcgcaa atgaactggc agctgaacga tctgaaagaa cagatccgcc
     1501 agggtaatgt tatctctggc cagcaactgg ctctgactgc ccgccagtat tatgaaaaac
     1561 aactcggtag ccttgagaaa acaatcaatc aggcaaacgc acgttgaaaa ctgacgtcag
     1621 aacaggactg aaaaaatgaa taagttaccg gaaaattcag accagacgaa aaacgtcaaa
     1681 aaatcaccat ccataacccg catagccacc cggggaatta ttaacatttt tgttccgctg
     1741 atggagactc gcctgataac gaattctgta cagcgcatgc tggaacaaca aaaacgcaga
     1801 ctggacagat ttcgtcaggt aaataataaa aaggcgcaac tccacctcac gtgggaagaa
     1861 gctctgcagg ccagccatat gtcagtagac gatctggatc gtcgtttccg ccggcgcaga
     1921 acagtatggc ggttatgctg ctggagtctg ctggccatcg ctttattcct cagcggtatg
     1981 ctctttgccg cctcctcatt acctttgata acacaggtac gcgccatcag cactcttgtg
     2041 ttgattctgt caggcgtcgc tctttgtgct tccagagctc ttatagttac ataccgcctc
     2101 tggcagttgc atgaacgaaa agtcagcgaa cccgagcagg ggacttttcg tgattttctg
     2161 aacgaccgca atggctggcg caatgccaca ttaattgccg taaccagtaa acaatactga
     2221 tcacgtccag atgccagtgc cactggcatg tcgccatccc ccttaattaa atcctgtgtg
     2281 gcctttgccc atacgcaatt ctattccgga gttactgtga aaattttact tcgcgcactc
     2341 tgtgcaggcc ttgctatatc ctcactgccg gcaatggctt cagtgaccta tcaggatatt
     2401 gtgtctgccg caaccaatcc tgacgatctg tcccgtcagg cgctggtcac gatattcggt
     2461 gatgtggtca ccaatccact gtcaaccagc gcccccaccc tgatcggaag catgttcggg
     2521 gcgttcaaca gtatcatcgc cgtacttgcc gttgtctggt ttatgttcat tggtatccgt
     2581 catgttgtgc gttcggggca tcagggacag gtattcagca ccggacgtga tatcgtcggc
     2641 acactgagtg tcgttgcagg ttttctgatg atcgttccaa ccggaaatgg ctggagtatc
     2701 gctcagctca ttatgttatg gggcgcatcg ataatggggg ttgggtccgc caatgtgatg
     2761 gtgcaacttg ccgctgacaa tatcgcaaat ggttattcaa tgactgtaca gccagttcag
     2821 gcatccacac gaacagcagc gcgtggcatt tttgaaatgg aactgtgtaa gtatgcggtt
     2881 aacgcaggac taaatgattt taatcagacg gccaaatcct caacttcact gatgacagaa
     2941 tcagctaaaa ccgccagtgg taattacact gtcacggtat ccaatggcag cggtatctgt
     3001 ggtacagcca gcctgtcagt agaaggaaac ggaacaacag atcaatcgac gattggaaaa
     3061 ttctttaatc ccttcagtaa aaacgaatac agcggtgtta tctcagcaca acgtgcagcc
     3121 atggataata tgattagcga catggacaat gctgccagtg agtttgtgac aacatttctg
     3181 gaaaaaagaa attctggcaa cggaacactg ccggatatcg agacacgaat tcagaaagca
     3241 gccgacgaat acgaacgtgc tgtgcaaaaa tcgctaccca ctgataacgg agagcaatcc
     3301 agaaaagagg ctcttaaatc ttatctcaca acctacgggt gggtaacgct tggcgcatgg
     3361 tatcagacct ttgccactgc taaccagcgt ttggcagagc tggctgacag agctccagct
     3421 gtaacttcaa tgtcatcact gggagaagtt ggtgatactg acttattcag tgcggtcatg
     3481 agtgcctata ggacgcaact gcagaattcc tcctatacgc caccgctggg cacagtagta
     3541 tcatccagtg aacaacgcat ggcaaatgca caagatccga aatctgcctt gtctgaaatt
     3601 atgggccccc ttgttagtct cactaacagg attgcaacgg aaacaagtgg cacaggcaca
     3661 accagtgctc aaattaatcc tctgataaaa atgaaaaata tcggagatat aacaatggta
     3721 agtgcagaag gaatctggac tatatataca acggccagag ttgtcgttgc cggtggcaaa
     3781 gattcaattc ttgtaaaatt ctttaactca ctaacaggtg ccgcttcgat gcttaccgcg
     3841 ctgtttgaag cacttgcacc acctgtttat tttcttttgt tcctgatgtt ctgtgctgga
     3901 ttttccttat caatttacct gccattcatt ccgtttattt tctggatgac aggcatcggg
     3961 aactggatag tcagcgtatt aataggatgt accgccgggc cgctgtgggc tgcaacacat
     4021 ctcgggacat cagaagacag aggaagtcgg gcagcctacg gttatatata cttaattgat
     4081 agtatgataa ggccgcccat catggtcttt ggttttttct ttgcttctgt agccatcatc
     4141 gccgtaggca caatactgaa tgcattattt ggcgctgccc tggttaacgt acagttcaat
     4201 tcgctgaccg gtattttcag tcttgctggt ttcttgctga tatacgccag aatttgtaca
     4261 accactgtag ctgccatatt tgcccttcag gcctacctac ctgatcatgt tattaacttc
     4321 ctagggggac gtgatggagc caacacgctt ggcagcatgg caaattctgt taaagaaata
     4381 tttattggag gaagtcgcaa tatccgacat actcctggaa tgaaaacaga tcgtcttaaa
     4441 gataatacaa aaggaagtga cgacaaggac ggaataaaag gataatatca atataaatat
     4501 tgcacaacct ccccatcggc attaaaataa tagatagtac gaggggaggc attatatgaa
     4561 agctaaaaaa gaaacaacag acagatttcc aacctggtgg ctattttatt atgtattaag
     4621 aaaagcttat ttctttcttg gaataccttt ttttctgttc tgcgcattag gctttaccga
     4681 aatgctatgt tctgatcgtt attccggaaa taaggtcgaa gattatgttg tcacttttgg
     4741 tagttggttc ttattacttg cgccaggcat atggatgtac tcacgtgcaa aaacaaggag
     4801 agaaaaaata agaaaagttg tacaaacaat aaaagagtca ggcttctact ctccagagaa
     4861 ggggtatgag ggattatctt taacccaggg agcttatttc ggcattgacc ttaaaaatgg
     4921 cacaatgttg tatgtccgta tctatccagg caacattatg gatgttatcg gttttgacat
     4981 tcataatttc actcgtactg taacagacga caaaaaactg gaaattcata cgaaatacat
     5041 caacctgcca atggttcctg ttccatcatg gtgtactcat ccagaaaccg catcaaacac
     5101 catgcacgct atggcaagtc gaggatatga ttaccctgtc gactttcctc gcctgattca
     5161 ggaaaagcgt aaggagtggg agcagattgc aggcatgcct gtagccgaag tattttaaga
     5221 taagctccct ccataacagc cggttcaaat gagccggctt ttgttcatta caacagataa
     5281 cttatgccgt taccgataaa ttcgaccaag tgctttaatc gctgcaattt ttatttccat
     5341 atcatcactt tgactcaatc tatgtaattc acgagtaatg ttctccgcct gacaccggcc
     5401 ttcacctaat gcatagatcg cagcaagtgt aacgtctttt gatttactct tcatcatttc
     5461 aaacaagaaa gtattagcta tcgcaggagg catgctcata atcaatcctc ttcatgaaat
     5521 aaacaatggc actcacttac aaacaggagt ccactatcgc aagcgactca acttagcaat
     5581 atctctcaat ttgaatgaaa aaaccagatg gcaagtgaca gggtggattt gcccctatat
     5641 ttccagacac ctgttatcac ttaatcccat aagctaacct tgtcacaaac gggcgcagaa
     5701 cagcctgcca gacagacaac aaaacaaaag tccaagataa aacatgaata ccggcatctc
     5761 aatgcgaaga aataatctcc gtagaaaccc ccatgagaaa aatatcatat gggagaaaat
     5821 cgcaattatc acagccagcg cattcattat tttcgctgtg ctctggcatt aattattttc
     5881 aacacaaaaa ggaaaaagca atgaatctgg tagacgcctt tgtcaaaaaa gtaatatccg
     5941 ggccatatga agaatatgga aaatggtgga tagatgtcga atacatttca tggggtgttc
     6001 ccgggaaaac aagactgatg tttgactcaa aggagcaggc actggaagtt aaggaagggt
     6061 acaaattttt aacatgagat ctctttttgt taaagggcag gaagcggtca cctcctgccc
     6121 ttagttacgt ttccaggctt atcagtcgat aagttcccgg atcagttcca gaacagcaat
     6181 cagcgcgttt gcaacggcaa ctactgccct gagaacgatg attaacagat taatcatccg
     6241 cttctccatc actggagaaa gaaccccgtt gttcgctctt acactgcctg tcacaacggt
     6301 ttctatagtg tcagttatag acagttcttc tccgaccaga gcaccaaaac cagcaccacc
     6361 tgatgttttg gccaggactt gtaacctgtt cttcccgctg aacaatatgc cggacagaac
     6421 atgcctcaga gctgacatgc cgagtataca cttatccagt acttgcagaa atataattcc
     6481 agacgtacta tctacaggtc agttatagac agttcttttc cgaccagagc cacccactct
     6541 gtaacggaac agtaaaaaaa ccgacctcac cagtcggttt ttttacatct gttgtttctc
     6601 aaaatccgcc cgacatgccc gtcacaccgg ggaaactcat gctttgaatc agaaccaacc
     6661 atctcaactt tgccgtctcc tgaaaaaact ggcacattca gttcgataac tacccataaa
     6721 tacagagaga aaatccgatg tcagaaaagt ggagtaaaga attcgactct tgctttcagg
     6781 tggggagcat agaagaaatt gccgatgctc tcgagaaacg cagcggaagc gaggaaaatg
     6841 cgttagccat gctggcaatg acagccttca cactcatgac ccgacgtggt atctgcgaaa
     6901 tgcagaacgt ggccccggat ggcaaaggca ttcggctgga gctaatcggc ttcaggggaa
     6961 atgaaacaat accggatacg cttcattgaa gaagaaaggg cgcaatgagg tcagccttcg
     7021 caacaaagcg gcggcagaaa acctgccgcc gtgttgcccg ttaacgttta acttcgtagg
     7081 ctaacgttgc cacaagcact gtgtttccct gctggagccg gagtacgcaa agcgaatccc
     7141 tcaccatcca gacaaaacac agaatcgtca cacagatgac gattaacatc attaaaaacg
     7201 ttcgctgtgg catcatagcc cctccattgc cttacggcgg ttatcgggct actataatgt
     7261 tgtcgaagca ttacagtaga gcccttggtt gattgataaa aaaatctcca ggggctttct
     7321 gctgtatacc gtccacaaaa cgctttcggc atacagcctc aagcgcccgc agctattcta
     7381 acgactttcg tatctgtaac aatcctttta ttatttgaat gattcagcgt tgtttttgac
     7441 gttaacaacc aacccaatac atggttctct acttcacaac aacgcatgtt atgctgattt
     7501 tagttaataa aagaatcccc ttaggagatg gaaatgtcgc aattaaaaca acctattttc
     7561 ctgaaaaaga taaaaaaagt catcaacaca atccctgggc ttgaagagca aatctttgca
     7621 tgccgaaata aaaaacgcag cgacaatcca ttattattta tcgacagaaa agatgaagaa
     7681 cgcatactaa tgagtcgtct ccagagccaa caaaaaaatg aagaactggc ttctaaacta
     7741 gaatctttat ttcatggaaa cgaattatcg tcaccacaca gtattttgtg ttttatatac
     7801 tggcgctata caaagaaaat ttatcgccta agcgaggaca tcatcagtga tgtggctaac
     7861 acctatgttg ataacattcc tgcacagacc cttaaggaac tcccttcatg gagtatctat
     7921 gtttcagccg aaaatctgca tactatactt cctacgtcct atccgataca tggatttttc
     7981 ttttatccat ttttggacaa aaatggaaat attatccggc ttttcattat tgatgatctg
     8041 aaacaaagcc aaggaactac agggctaaaa gaaaagaatg ttgacgtcgt caataatatc
     8101 atcagaataa aggactctcg tgaaggactt cttgactcca gaaagatgga gtgtatcgat
     8161 ggtgaagtcg tcgtaacagt taacgaaaag ctcaaagatt tcagagacag ggagtttaat
     8221 cttcttaacg cacagatatc aatggtgctt tatatctgtt cacagatcaa tgatataaaa
     8281 gaaaaaaatc aatttaaacg gtcagaaaag cacaaaaagc atgttcacac tcaccatgaa
     8341 ctaccagcac aaaacattcg ggagtgggac gttggtattc gaatggggca agccatacgg
     8401 caatatcgcc aagcagaacc cacagggaaa gagcggacaa caataggtag caaacgtcct
     8461 catatacgcc ggggacactg gcatacatac tggacaggaa gtaagaaacc agaactagcc
     8521 catgagcgta aacctagatt aatatggtta cctccggtac cagttaattt agaagacgta
     8581 aacaaactac cggtggtaat aacgcccatt gataaatgac ctgaaaagta tcttttccgg
     8641 taggatgtac acatatctaa attatcaggc tactgtaatg ttgttcatgc attacagtag
     8701 agcccttggt tgattgataa aaaaatctcc agggggcttt ctgctgtata ccgtccacaa
     8761 aacgctttcg gcatacagcc tcaagcgtcc gcggctattc taacgacttt cgtatctgca
     8821 acaatccttt tatcgcacgg ctaactcctc tggttagctc ttatcacctg aaaataaatt
     8881 tttaaccttt ccgtttgatt taaattccag aaatggagac tttgtcatgt cttacaatcg
     8941 acagcctgtc gcagaagatc ctatgcagat atggggcgct gttggtgtcc ttcttatcct
     9001 gctgctattc gtcatatggt tattcttgcc ggaagtggtt tacgccagct gcctgattct
     9061 tcatacctta tggggactgg ttgactgggg accatttcat aactatgcag caccacgata
     9121 caacctgctt gccatgaccg gtaacaacgc cgccaacata agctatagcc agtgggttaa
     9181 cgtcatggag cagaccatag gtattttgtg gatgtacctc ttacctgtga cactctggtg
     9241 tttgtgggaa tggtatcaac atccgggaca aagccgtttc acccgcagac cagtagatat
     9301 tacccgactg ccacacatct ttgcttcgtt gtcccctgct atcgcccctg tactggaaga
     9361 tggtgatccg gaaaaactct ttcatggtgg caaacgacct gaacggcggg ttgctctcac
     9421 cccggaagca tttgtcgaac agcatactct catcaccaat atgcagctcg atgttacagc
     9481 tgcaagacga tgtttcatgg cccagttggg taaaccttta acgtcctgga aagacatggc
     9541 accacatgaa aaagcgctct ttgccatatt tggccttcag tatttcctgg atgatcgtaa
     9601 ggcagcatta aagctcatgg atacactgaa cctctcatgc agaattaaaa gcaaacggga
     9661 cagtgggaaa ttctgtaccc ctgtttattc actggccaaa tctgcattca aaagagtgat
     9721 taaaagcgac ggagcacagc aatggctgaa acaacatcgc tacgtccgca gtgggctggt
     9781 ctggctgtat gcacatgacc tgcggcttac gccacccaac tggatttggc tgaaaggcgt
     9841 ggacagaacg ctgttttatg cattacaccg cgccaatacc acgaaaggat ttattgaagg
     9901 cgctggcgtg gtggccgttg cacgtgcaga ggccgaagcc atgcgttttg gccttccctg
     9961 ccctgaaccc tgtgttgatg aagcggtgga aggcttacgg cgggatatgc tcagcctcgg
    10021 tcttatctgg gatgaaccac aaccagatcg cgaccggaaa cgccgcattc tgaccaactg
    10081 gtccctcacc gacgacatcc tgccccgcac acctgctaca gacaacgaat tctgattatc
    10141 cggagaactg aaaatgacaa tcgagtactt tgcaacacgc attgcaaaac atatttccgt
    10201 gcaggcaatc tggccgggtg gcaggacaga aattattgcc gtccttccac aggatgccct
    10261 gtcagggctg gtaacacgaa acaatcaact gattcatata gcctttgacg ccatgggcac
    10321 tcgtaacaga gaaaccgtcc tgattgacaa gcaggagcat aacgctgacc agatacttac
    10381 agctattgat tcctgtctgc gccttgaata cctgatggaa cgccgcttca gcagaacgcc
    10441 tttgttccgt gcaataatcg ccagcgttgt actgttcgta atggcaatga ttgccgtctc
    10501 tctgttccgc tatgttgatc gtgtattctg ggatgataca acgcctgaag cagttcagac
    10561 ggcaggtgag ccacgactgc tcccccctca cctgaatcac accgtgccat taaatgaagg
    10621 cattcaactc ccggttaccc cgaaaaaaga cgtccaggta ccggagaaaa cactcacaag
    10681 caaaaatccg gaagccgcag ctgcccgaca taatctggct gctgttctga agcggaacgc
    10741 cgatcggggc atgtttaccg tcaacctgtc atccggtcat gaacgaaccc tttacgcatt
    10801 tctcgatcct gcctgtccta actgcagact tctggagccg gctttaaaac gcctggccag
    10861 cgacttcaat gtcgttatct acccggtttc agttattggt ggagaggaat ccaccgaccg
    10921 tgtggcacca ctgctgtgtg aaaaagatgc tcagaaacgt gctgccggct ggcaccgact
    10981 gtattccgca gataacggaa tgatgacgcc atcagaagaa acaacgcctg cagatgaaac
    11041 atgcctgaaa gcggccagag ctgcgattga tgttaacaat gtcgccttcc gtaagttcgg
    11101 atttgccggt accccatggg tgctgtccga caccggctgg catctgccta caggaattct
    11161 gcaggaaact ggcacgctta atttattcct gaaaaccacc gacagcgagt ctggccatga
    11221 gtgaacatcg cgttaacccc gagcttttac accgtacagc ctggggaaat cctgtctgga
    11281 atgcactcca gagtctcaat atctatggct tttgcctggt ggcaagtctt gtggccagtt
    11341 ttatctggcc acttgcatta ccggcatgtc tgttgttcac actgataaca atgctggtat
    11401 ttagtctgca gcgatggcgc tgccctcttc gtatgcccat gacgctggaa tgtgctgacc
    11461 cgtcacagga cagaatgatt aaaagaagtc ttttcagctt ctggccaacc ctgttccagt
    11521 atgaggtcat tctggaatca ccggccagcg gcatattcta tgttggttat caacgcatca
    11581 gggatattgg cagggagtta tggctgagta tggacgatct gacccgccat ataatgtttt
    11641 tcgccaccac cggtggcggt aaaacggaaa ccatctttgc ctgggcgatt aacccacttt
    11701 gctgggcaag aggattcacc cttgtcgatg gcaaggcaca gaatgatacg gcaagaacga
    11761 tctggtatct ggccagacgc tttggacgtg aagacgatgt ggaagtcatt aacttcatga
    11821 acggaggaaa atcccgcagt gaaatcattc tgagtggtga aaaaacgcgc cctcagtcaa
    11881 atacctggaa tcccttctgt tacagcactg aggcattcac agcggagaca atgcagtcaa
    11941 tgctgcccca gaacgtccag gggggagagt ggcagtcccg ggccatagcc atgaacaagg
    12001 cactggtatt tggtactaaa ttctggtgtg tgcgcgaagg caaaacaatg tcactgcaga
    12061 tgctccgaga acatatgact ctcgaaggga tggccagact ttactgtcgt ggcctcgatg
    12121 atcagtggcc ggaagaagct atcgcaccat tacgtaacta ccttcaggac gttcccggtt
    12181 tcgacctgtc gctggtcagg acaccatcag cctggacgga agaacccaga aaacaacatg
    12241 cttatctctc cgggcagttt tctgaaactt tcagcacatt cacggaagca ttcggggata
    12301 tttttgctga agattccggt gatatcgata tccgtgacag cattcacagc gacaggattc
    12361 tgatggtaat gatccccgct ctggatacat cagcgcatac aacgtctgct cttggacgaa
    12421 tgtttatcac ccagaaaagt atgattctcg cccgtgattt gggctaccgt ctcgaaggaa
    12481 ctgattccga tgcactggaa gtcaaaaaat ataaggggcg tttcccttat ctgtgtttcc
    12541 ttgatgaagt gggggcatac tatacggacc gtattgctgt ggaagccact caggtgcgtt
    12601 cactggattt tgctctgatt ttgatggcac aggaccagga acgtatagaa ggacagacaa
    12661 cagccacgaa tactgcaaca ctgatgcaga acacggggac aaaatttgcc ggcaggattg
    12721 tcagcgaggg tagtacggcc agaacgctga aaagcgccgc cggtgaagaa gccagagcga
    12781 gaatgaacaa cctgcagcgt caggacggca tatttggtga atcatggatt gacagcccac
    12841 aaatcagcat cctgatggaa agcaagataa atgttcagga gttgattgaa cttcatcctg
    12901 gcgagttctt ttctattttc cggggagaaa ccgtaccatc agcatcattc ttcatccctg
    12961 acgatgagaa aagctgcagc agcgatcccg ttgttataaa caggtatatc agcgtggatg
    13021 cgccacgcct ggaccgtatc cgccgtcttg ttccccgtac tgctcaacgt cgaatcccgt
    13081 caccggaaaa tgtcagtgcc atcattggcg tcctgactgc aaaaccctcc agaaaaagac
    13141 gaaaaattcg cacagagccg cacacgatcg tggatacatt ccagcaacgc attgcaggac
    13201 gtcaggcggc tatggcaatg ctggaagagt acgacacgga tatcaacgca cgtgaaagtg
    13261 ccttgtggga aacagcggtg aatacgctta aaacgaccac acaggaagag cgacgaattc
    13321 gctatatcac gctgaacaga ccagaactac ctgaaacaaa agaggaaaat cagatatcag
    13381 tgagagcaga aagagcaggt ataaacctgt taacgcttcc acaggacaat acccccccga
    13441 cagagcgtcc tgtaaacggg tttcatcata aaaaaaccaa ccgtccagac tgggacggaa
    13501 tgtactgaac tgtgaccata cttcggtctt cccgcaaaaa actatttaaa aatgctggta
    13561 ctagatacca gcattcagat tacagattaa ccgcatcatt gcaggtacat atggaacgtc
    13621 taatttttat attatttaac cctaggtcat ctaattttga ccgaagctgt tcatcagatt
    13681 gttcaaataa aggccccaac acaatctctc taattgacgt aatattgtta caggcaaatt
    13741 tcaccttagt ataatacgca attccgtaat cagtactacg ttcttcaagt ttgtatggtg
    13801 gggatttatg tttatctact tcaataagtc ctcggacctc attttcacat tcaaaactct
    13861 ctctcttata taatgaacat agtctgataa gatatgcatg aagtatgcca tgtgcatatt
    13921 caatgtttaa acttgagcgc ttaatataat catgcaatgc ttctcttaca tattctcgaa
    13981 acagttcttc tgaatagata acaggatgaa aaacaactct accactaata ggttgaaaat
    14041 gttcttcaaa ccgggcaaga atatgatatt gggttataat attcttatca taaccaatag
    14101 caacgccttt gccattatca ccatactcgc tccacatgtt ggaacgatct ccatcagaac
    14161 agaaacaaca aattaatatc ttacaatttt cgtctatctt attaggtgat acttcttcta
    14221 atagtctaat gcaatctgga aattcctcat ttattattct cttgactatt tcatagccag
    14281 ccgagtattc ttccggatca ttcatattcc gaagatcggt aaaccataat tcttggtttt
    14341 caatcatttt aaaaaaagtt tcaacacttg cataacgaaa agcaagatta gtgctcataa
    14401 aacatgactc cgtcaataga gttacttaac attgcctaac atatttattt ttaccaccac
    14461 atccatattt aatattggtg atttttcatt ctatcacgaa actaataaag cttcatctgt
    14521 catctcatcg gagaaggcgg ttcccagagt ttatcctgct ccggcggagg caatgacgca
    14581 tcatcattgc tgttcgccgc gatataatcg cggtagtagc gttcacttcc ttcacggtga
    14641 tgatccacgt ccggtcgcgt aatcctgtag tcttcgtgac cggtacggcc tgtcacatta
    14701 tgccaggcaa ccatttcagc aaagacctgg ttaaattgtt tgtcgtttcc ttctggttca
    14761 aaacccatgc gtggatcacg cccgaataat accggtgcaa tcaaatccag tttatctgcc
    14821 agcgcattgc ggtcatggtg attacggaaa acaaccctgt caccataatc cgtacagaca
    14881 aactctcccg tccgacggtc acggaaacga acactcccgt tcttctgcaa acgggcctcg
    14941 agatcaccgg tattaccgta taccggcgtt ccgcccggtt cacaaagcac gacacagcgg
    15001 tctgttgtcg ttgtgcgtga cttatcttta cggcgatcgc gataatccca gcctctcagc
    15061 tgcgatacag cagccctgtc cccctgagca gcctgaattt cgacccactg acggtaagag
    15121 ggtggatacc acttcccgtc agcaataagt ttctgccgct catccctgat gtcttctttc
    15181 agcctgatca atgcctgcat tcgctgaact tctgcaatgt gataatgcag cttacgtaat
    15241 gccggatcat cgtactgcgc acgaatgtgt gacttccgca gacggcatgc ctgatgaatt
    15301 tcccgacaac gttccccata acgcagatct ggtttacgcc attgttcacg ccatgccaga
    15361 tagcgggcac gcaggtcagc acgggcctca gctctggctt cacgtcgctg ccggcgcagc
    15421 attgggtcac gtttactgct gaccccgtac ctgtcactga cagccagctc cgggttatag
    15481 cgactttcag gttgcaccct gtcaaacaaa tctgctgggg cactcacaaa aggtccggcc
    15541 tgtggctctg cacgccccag tgtcagatca ggatgaatgc tgctggcctt caccggcgtt
    15601 tgctcatgat taaaggcatc aacgacaaca agcccgtgat gttgtttctg tagcatcaat
    15661 ccctgttgtg caaacaactc atgacaacgc tgccaggtaa actcaccctg tgtgatcatt
    15721 tcttcaatct gagggctaca atggctgatg ctgtatcccc acagactttc tttgtcagaa
    15781 aacagttcgc gtttgatagc cccctgcggc cccgtacctg tacgcatacg gtgccattct
    15841 ttatcggtcg caggacagct ctccatacga cgaccacgga agcgttctgt tggcgtaacc
    15901 tggcggaaaa tatctgttgg tacaggctgc cagccaccat cgagttgatt cacattgtcg
    15961 agcgtcagca gtgaccatac gctgtcagca cgaacgggag tctggttatg ctcataaccg
    16021 tcacagataa ccaggtgacc atgctgaaca cgcacccata atcccgcttc agccagtgtg
    16081 cggtgaatag cctggcagtt ttccagccgt ccttcccgtg ccatttccgg cagacgttct
    16141 ccaagatgac gacaggcata ctgctgcaga ctttctgttt cagcgatttt ttccgggcga
    16201 acatcagctg caataaagtc cgggttatag cgtccgggtg cgtccacctg gctgaaaata
    16261 tcttttggta ctggcgtgta gtcacccatt tttttcatga gtttttctgc acaccaggag
    16321 ggaccaaacg agtcaagctg tacaccttcc ctgttgcgat cccagccatc catcaccaga
    16381 aattttccgt ccatctgaga caggtaaaga ccatcttccg caagacgacg atgcagggat
    16441 aaccagtcat gtacaggttc actgcgtaaa ccagcgaccg ctgtctgcgc aacatactcg
    16501 cgaaaagttt gtttttttcc gcgtgtccaa gcgttctggc gatcacgttc aacggcagtt
    16561 ttgcgaacga tacgatttcc cggtgcatga acccagcaac cgttatccgg agcaaaacca
    16621 tgcttcagtt caagctcacg gcaggcacga ctgagttttt cctgactcca ggataaccgg
    16681 ttcagataac ccgtttcagg gtgtacccgg ttaacagcca catggacgtg cagattatct
    16741 gtatcagtat gcacggcaga gacatactga tggtcggcaa ggccgagtga tttcagcgta
    16801 tgacgtacac tgtcataaat ctgttccgga cgcgggcttt catgtgactg ccaggaaagg
    16861 atgtagtgaa aaacagggtc agtatcatct tttgcatagt gagcctgccg ggcaatgtac
    16921 tccatatctg cagcggcagt ttcaagcgat gtacagttgt gaaaacaggt cacaccataa
    16981 aagttaaccc attcgcagcc gtccttcatg acatcaacca gcgccacgaa tgattcattt
    17041 cgaagacgtg tcgcataatc aacaaggcga ctgaaacggc ttcggtatgg ttgttcagcc
    17101 tgcgaagaag aagataaatc cagttcctca tcggtcatgt catcccggac agagacgtag
    17161 gataccaggt cttcgaacga agacttgccg tcccttctct ttttcggaat gactgcattc
    17221 accggagaat cctcaacctt ccattaactg tttacgcaat gtatttgtaa gttcggttat
    17281 ggcaacaagt acttctgcgt attccttgtc accggttctt tttccctgaa caaacagatg
    17341 cttctgcatc cttcccagtc tcatcaactc cttcagaaaa gcatcatctg tccgggagtt
    17401 aattcgctta ttcagtgctg catttcggat gtaagcagat acagtaagtc cggaatcttc
    17461 agcattctta cagatagttt catcctcatc cggtgagaac cggaccgctc tcaggatcgt
    17521 cttttgccgt ctttcactac cacttctttg acgtttatca ctcacggtca gctccttact
    17581 ggggtgtcgg ggcgaagccc tgaccaggag gcaattgtat aatcgcgcgt gcgcggttat
    17641 acaattgcac atcctgtccc gtttttcagg ccattatagc ctgttatttt tgtgcatcat
    17701 agtcttatgc agacggcaga ggcagggcgc agccctgcca ggaggcagtt tttgatgata
    17761 aaatcagact tcaacatgaa acggagagaa agtgccaatg gcttctaaaa aattttactc
    17821 agatgatgac atccagctgg caaaagctgc cttatcggaa ttgccggatc tcaccgctca
    17881 acggaaaaca ttgcgtgatt ttcttgatgc cattcgtgat gacattattg tcctggtaag
    17941 gacaaagggt tatacactgg cagatgttcg tgacacgctc aaaaatgcag ggtatgaagt
    18001 aggagagaag gcacttcgtg acattattcg ggaagcagaa tcgaaaaaac catcccgccg
    18061 ttcttcctct aaaacagcac ctaaaaagac agattcaggc aggaaagatg gcatcgatat
    18121 gaacaacaat tgatgtgaca atgtcacatc gtgttctgag attatgatga ttacagaaaa
    18181 aaaagccccg gcataaatac cagggctttt ctgtttaatc agactcgtgg cggcaatgca
    18241 ccaggataat gccgtgcaat aatatcattc agtttttcaa caaggtcaga acgcttaaag
    18301 acaatgtgta cggtcccttt ctggtagcca cgcaccgtaa aatattcccc ttctaacagc
    18361 ttaccgacat tgtcgccgtt cccgagaaat tcaccatact gcgccccgat gctgttcctg
    18421 aagtcaggaa cgttgcgccc ctccagcaga taaaacggac gtaccaggtc atccagtgcc
    18481 tcccgtccgc tgcagtctag cgtcacacgc ccgttactcc agcggtacag caggttttca
    18541 ataatgattt tcttacccag gcggcaggga ttattggttc tgtaatccca tgacaatttc
    18601 cggtagacat caatcagccc ctgttcaaac gtctgcatct tgctggcatt aagatggcgg
    18661 aatgttgcca gtacattgtc cagggtgatt tcaggacagg tgtcgctcat cagctggctg
    18721 ttccactcat cacgctggca tgaactcatg aaggtgtaca tgcccgtatc tgtcatcagg
    18781 cggtcccaga ttttccggtc ggcaagacgg gtgatattcc ttttcgcttc ttccggttta
    18841 tccatgcgac gaacggaatc agtcacgcag tcttcaaacc cgtaaagaaa aatatcacct
    18901 gacgcatcga gcagaatcct gcgcgctgtc gccaggcatt ccatcgcctg catgaacagt
    18961 gcaatacctt cgttacgctg cgcgatgatg cggtcgatgg ccacagacgg aataacatct
    19021 gtcgtgccgg cagtattcag gtcagcagtg ggtaaagtct gattcatcac attgtctcct
    19081 catcgtcacg actgccgggt cagccaggtc atttcccgga ggagcgaagc tccttctgcc
    19141 gtccgggtct tcggggattt ccccgcagtg gacctttccg acgggggacg ctcaccacgt
    19201 cacggggcag ttgtccgggg gcagacgcaa aagtttttga cgcctgcggc aaaaagtttt
    19261 gtggctggta catctctccc ctggatgact gaaacgggat gtggtcgtgt atgtaccccc
    19321 ggaaagtccg tctgcggaga acaggccgca gtgcggcggc agacaggcag agacagctgc
    19381 gccatcaggc gcagtggcct tacacaccgt aaggtgtgca gggagcgccg catccggcgc
    19441 gactgaagaa aaacgacggc acaacaatgg catgtggtgg tcactggcac accactcccg
    19501 cacaggtgaa aacagagaaa accgcacgac atttacaacc aaaaaaagtg aaatttcttc
    19561 acttttttca cttatttatc gcgctgattt ggcaattgtg atgataaaat aaagtgaagt
    19621 tttttcactt tttcaaaggt gttttatgac tggctgggaa ctgcgaatct ggcgaaaaag
    19681 catgctctgg tcacgcgaaa aagcggccag agaatttggc gtgacgcaga gaacatggca
    19741 cgcctgggaa aatgcggaac aggtggacgt caccgtctgg aggaccaccc aggcactttc
    19801 agtccgtgac ctgctgcctc acatgcaggg gatgcgcaag gcagatatca tcaggcgtct
    19861 ggaaaatgaa ctgggggaaa cggcagagga tgtgtgagcg tgattcacag cctggcagaa
    19921 tgccaggcta ctgctcatca ggaaacagtt ttttcagttc ttcaccggaa agtccggctg
    19981 cctcctgaat ccgttccggc gtccagccca tatcccgcag ccggcaggct gcttcccgcg
    20041 ccacttcaag cgcacctttc ttaaaacctt ctttaaaacc ttcgtcgaaa ccttcgtcga
    20101 aacctttctg tataagacgt tctgcaatcg tcatactgtt ttctctgctc ctgctggcaa
    20161 aaggcccggt attttgccgg gctgtgaggt tctcatcagt gaatgatttt cttcagttcg
    20221 tcatcggaaa gtccggtagc ctgcttaatc tgctccggcg tcatccccat tttctgaagc
    20281 tggcacgcaa tagccagggc tttttcaagc gctccttcca tgcgtccttc catcctgcct
    20341 tcctggcgac ctttttgctc aagacgttct gcgatagtca tcagtttatc ctcatgctgc
    20401 ggcagacgct cagccagcgc ccgcataaat tccggtgata cagagtcccc tgcgttaacg
    20461 atgtaatgaa tcagaacacg cacctgctcg tctgaaattt ccaccatgac ctgcgggagt
    20521 ttgtccagca gctccatcat gtcacgatgg cggatatgct tcatcagcag cgtcagtgcg
    20581 gccatccggc gatggttcat gatttcattg tcatcaacga cagtgatatc aaccagcgga
    20641 aacggctttg tgtatatttt agccgcaagc gccggattct caaaacagtc cagccagttc
    20701 atgctgtacg gatacgggct gcgctcacca tgatagaaca gcaccggaat caccagtggc
    20761 aacgttttgt gctgctccag atgtctctgc atcgccgcca cactgtaacg taacatgcgg
    20821 aaaggcatgt acagattttc ggtgctctgg tgctcgatga gcgtataaac atagccgtct
    20881 ttactcccgg tggttttcac gctccagagg atatcgctgg cgtactgatg caggtccggc
    20941 tctacaaagg tggcgggttc cagcttcagc gtggacaggt cgcacaactg ccggtactcc
    21001 gcaggaagat gcagttccag aaaatctctg gcgacgtcgg gattcgccag gaacgaccgg
    21061 aacgcggcat catgcggcgt gggcgtcgtg gtgtttttct tcttactcac ggttcctcca
    21121 tcaaatcagc agcatcgcag caatcatcgc ccaaatcccc acaaccgaga ggacagcaat
    21181 tgcgttcaaa gccatgacaa caatagccgc gttctgagtc acggcgtatt tcttcagcaa
    21241 tctcttctgc ggaaaggtaa tcaaactcct cacgggccag ttccaggacc agtagatgtt
    21301 gttctgtcgg caatgtggcc acaaaattat caatagcagc actcaaacgt cgctgatata
    21361 gctcataaac aagaccacaa cggggcatta gttcttcgga taatagttcc agttttgagc
    21421 gaattctgtc agcgttgaaa gtgacttcat caaaagacat atgaaaagct ttgtcagtat
    21481 caaaacaaga cttcattgtt atctccagcc ctcccgggcc ctgaatgaaa gagaatctgt
    21541 ttcccttttc tgttttttgt ggagacacca tgcaacacag acgtcgtgat gtttttcatc
    21601 ttgctcacgg ttcctcctgg taaagtacct gaacacatcc tgccacctgc acaggcagac
    21661 ggcaggggtt aatcagtgac agacccgttc aatttcaagc gcgcggagca ggtggcccca
    21721 ttcgctgact ttcatggcgg ggatacgtac ccgcttctgc tcactgcagc gtctggcgat
    21781 atgaccgctc atcgccagga cgtccgggtt aatctcatcc tgtgccagct cctggcgtct
    21841 gaagcgggta tggcggtgca ggggtttatt ctcccgcagg cactcttcac gcagacgctt
    21901 tacatactcg gcggttgcga agataattga tggtttcatg gcataatctc catagatagt
    21961 gattgagatg ccggacttcc tggcggggaa gcgccggcgg agagaggcag ggtttatcgc
    22021 cctgcctttt tttcatttcg gacagcgacg cagtgccagc gcgagcgctt tcatcttcca
    22081 gccttccggc gtgcggtaca tcaccttcca gtagccgtcg atgaacacat aatggaagcg
    22141 cacctcttca gtgctttcag tggcaatagc ctccatgaaa gaacggtact cacgcgcctc
    22201 actgtccatc cggaaaccac tgtcacgtcc gtaataagcc gtcacaccgt ccaccgggtt
    22261 ctgaagcgta tgcccggcgg gtttgtcgca acggggaccg aggcaacgga tatcaccgtg
    22321 ttcaaccagg gcttcggcct gctgaccggt ggcatagtgg gtatgcagga tgcgaccgtt
    22381 ccaggtcggc tcaccgtgca ggtggcagta aacggtacgg aacaccccgt cgcggcaaaa
    22441 aacggaaatc gtgctgctga cagtcatgaa aacgtctcct tctgctgaat caggtcgccg
    22501 ggccgaccgg ttgctccccg gaggggcgaa agccccttct gccgtccact tcttcggggt
    22561 ttctccccgc agtggacctt tccgatgggg cgtccgtcac cacgtcacgg ggcagctgtc
    22621 cgggggcgga cgcaaaattt tttgccgggt ttatggctaa aaattttgtg gacggtatat
    22681 cttcccctgg actgctgaaa cgggatgtgg ttactccagc ccccggaaag tccacagcgg
    22741 gaaacaggcc gcaggggtgg cggcgga
//
LOCUS       DAEEBB010000061        22595 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEEBB010000061 DAEEBB010000000
VERSION     DAEEBB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.061"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            238..1344
                     /gene="ompC"
                     /locus_tag="KLF07_002212"
     CDS             238..1344
                     /gene="ompC"
                     /locus_tag="KLF07_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD9832220.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1456..2511
                     /gene="apbE"
                     /locus_tag="KLF07_002213"
     CDS             1456..2511
                     /gene="apbE"
                     /locus_tag="KLF07_002213"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBD9832221.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2585..3649
                     /gene="ada"
                     /locus_tag="KLF07_002214"
     CDS             2585..3649
                     /gene="ada"
                     /locus_tag="KLF07_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBD9832222.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3649..4299
                     /gene="alkB"
                     /locus_tag="KLF07_002215"
     CDS             3649..4299
                     /gene="alkB"
                     /locus_tag="KLF07_002215"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBD9832223.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4375..6018
                     /gene="yojI"
                     /locus_tag="KLF07_002216"
     CDS             4375..6018
                     /gene="yojI"
                     /locus_tag="KLF07_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBD9832224.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6236..7882
                     /gene="mqo"
                     /locus_tag="KLF07_002217"
     CDS             6236..7882
                     /gene="mqo"
                     /locus_tag="KLF07_002217"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBD9832225.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8191..8679)
                     /gene="eco"
                     /locus_tag="KLF07_002218"
     CDS             complement(8191..8679)
                     /gene="eco"
                     /locus_tag="KLF07_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBD9832226.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            9087..9581
                     /gene="napF"
                     /locus_tag="KLF07_002219"
     CDS             9087..9581
                     /gene="napF"
                     /locus_tag="KLF07_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBD9832227.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9571..9834
                     /gene="napD"
                     /locus_tag="KLF07_002220"
     CDS             9571..9834
                     /gene="napD"
                     /locus_tag="KLF07_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBD9832228.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9831..12317
                     /gene="napA"
                     /locus_tag="KLF07_002221"
     CDS             9831..12317
                     /gene="napA"
                     /locus_tag="KLF07_002221"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBD9832229.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12324..13019
                     /gene="napG"
                     /locus_tag="KLF07_002222"
     CDS             12324..13019
                     /gene="napG"
                     /locus_tag="KLF07_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBD9832230.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13006..13869
                     /gene="napH"
                     /locus_tag="KLF07_002223"
     CDS             13006..13869
                     /gene="napH"
                     /locus_tag="KLF07_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBD9832231.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13866..14315
                     /gene="napB"
                     /locus_tag="KLF07_002224"
     CDS             13866..14315
                     /gene="napB"
                     /locus_tag="KLF07_002224"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBD9832232.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14325..14927
                     /gene="napC"
                     /locus_tag="KLF07_002225"
     CDS             14325..14927
                     /gene="napC"
                     /locus_tag="KLF07_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBD9832233.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14946..15563
                     /gene="ccmA"
                     /locus_tag="KLF07_002226"
     CDS             14946..15563
                     /gene="ccmA"
                     /locus_tag="KLF07_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBD9832234.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15560..16222
                     /gene="ccmB"
                     /locus_tag="KLF07_002227"
     CDS             15560..16222
                     /gene="ccmB"
                     /locus_tag="KLF07_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBD9832235.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16264..17001
                     /gene="ccmC"
                     /locus_tag="KLF07_002228"
     CDS             16264..17001
                     /gene="ccmC"
                     /locus_tag="KLF07_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBD9832236.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16998..17207
                     /gene="ccmD"
                     /locus_tag="KLF07_002229"
     CDS             16998..17207
                     /gene="ccmD"
                     /locus_tag="KLF07_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBD9832237.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17204..17683
                     /gene="ccmE"
                     /locus_tag="KLF07_002230"
     CDS             17204..17683
                     /gene="ccmE"
                     /locus_tag="KLF07_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBD9832238.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17680..19623
                     /gene="ccmF"
                     /locus_tag="KLF07_002231"
     CDS             17680..19623
                     /gene="ccmF"
                     /locus_tag="KLF07_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBD9832239.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19620..20177
                     /gene="dsbE"
                     /locus_tag="KLF07_002232"
     CDS             19620..20177
                     /gene="dsbE"
                     /locus_tag="KLF07_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBD9832240.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20174..21226
                     /gene="ccmH"
                     /locus_tag="KLF07_002233"
     CDS             20174..21226
                     /gene="ccmH"
                     /locus_tag="KLF07_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBD9832241.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21261..21908)
                     /gene="narP"
                     /locus_tag="KLF07_002234"
     CDS             complement(21261..21908)
                     /gene="narP"
                     /locus_tag="KLF07_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBD9832242.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22228..>22595
                     /locus_tag="KLF07_002235"
     CDS             22228..>22595
                     /locus_tag="KLF07_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD9832243.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5058 a         5669 c         6428 g         5440 t
ORIGIN      
        1 gcttgatgtt aggtgcttat ttcgccattc cgcaataatc ttaaaaagtt cccttgcatt
       61 tacattttga aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc
      121 gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta
      181 atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg
      241 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct
      301 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg
      361 cactatttct ctgacaacaa gtcagaagac ggcgaccaga cctatgtacg tcttggtttc
      421 aaaggcgtaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc
      481 cagggcaata cctctgaaga caacaaagaa aactcctgga cccgtgtggc attcgcaggt
      541 ctgaaattcc aggatgtggg ttctttcgac tacggtcgta actacggcgt tgtttacgac
      601 gtaacttctt ggaccgacgt actgccagaa ttcggtggcg acacctacgg ttctgacaac
      661 ttcatgcagc agcgtggtaa cggcttcgcg acctaccgta acactgactt cttcggtctg
      721 gttgacggcc tgaactttgc tgttcagtac cagggtaaaa acggtagcgt aagcggcgaa
      781 ggcatgacca acaacggtcg tggtgctctg cgtcagaacg gcgacggcgt tggcggatct
      841 atcacttatg attacgaagg cttcggtatc ggtggtgcga tctccagctc caaacgtact
      901 gatgatcaga acagcccgct gtacatcggt aacggcgacc gtgctgaaac ctacaccggt
      961 ggtctgaaat acgacgctaa caacatctac ctggctgctc agtacaccca gacctataac
     1021 gcaactcgcg taggttccct gggttgggcg aacaaagcac agaactttga agctgttgct
     1081 cagtaccagt tcgacttcgg tctgcgtccg tctgtagcat acctgcagtc taaaggtaaa
     1141 aacctgggta ccatcgctgg tcgtaactac gacgacgaag atatcctgaa atatgttgat
     1201 gttggtgcta cctactactt caacaaaaac atgtccacct acgttgacta caaaatcaac
     1261 ctgctggacg acaaccagtt cactcgtgac gctggcatca acactgataa catcgtagct
     1321 ctgggtctgg tttaccagtt ctaatctcga ttgatatcga acaaagggcc tgcgggccct
     1381 tttttcattg ttttcagcgt acaaactcat ttttttggtg tactcttgcg accgttcgca
     1441 tgaggataat cacgtatgga aataagcttt acccgcgtgg cactgctggc tgccgcgctc
     1501 ttctttgttg gttgcgatca aaaaccacaa cccgccaaaa cccacgctac tgaagttacc
     1561 gttctagaag gcaaaaccat gggtaccttc tggcgtgcca gcatcccggg cattgatgcc
     1621 aaacgcagtg ccgaacttaa agaaaagatt cagacccagc tggacgccga cgatcagctg
     1681 ctttcgacct ataaaaaaga ttccgcgctg atgcgcttta acgattcgca aagtttatcg
     1741 ccgtggccgg taagtgaagc gatggccgat atcgtcacca cctcgctgcg cattggcacg
     1801 aagaccgatg gcgcgatgga tataaccgtc gggccgctgg tgaatctgtg gggctttggc
     1861 ccggaacaac agtcggttca aattccgagc caggaacaga tcgatgcgat gaaagccaaa
     1921 accggcttac agcacctgac ggtcattaat cagtcgcatc agcaatatct gcaaaaagac
     1981 ctgccggatt taaatgtcga tctctccacc gtcggtgaag gttatgcggc ggatcacctg
     2041 gcacgcttga tggagcagga agggatttcc cgctatctgg tgtcggtggg cggcgcgctg
     2101 aacagccgtg gtatgaacgg tgaaggcctg ccgtggcggg tagcgattca aaaaccaacc
     2161 gataaagaaa acgcggttca ggctgtggtg gatatcaacg gtcatgggat tagcacctct
     2221 ggcagctacc gtaactatta cgaactggac ggcaaacgtc tttcccatgt tatcgatccg
     2281 caaaccgggc gtcccatcga acacaatctg gtatccgtga cggtgattgc tccgacggcg
     2341 ctggaagccg atgcctggga tactggcttg atggtactcg ggccggagaa agccaaagag
     2401 gttgttcgcc gggaagggct ggcggtttat atgatcacca aagaaggcga tagctttaaa
     2461 acctggatgt caccacagtt taaaagcttc cttatcagcg aaaaaaatta aagcgcaaga
     2521 ttgttggttt ttgcgtgatg gtgaccgggc agactaaagg ctatccttaa ccagggagct
     2581 gattatgaaa aacgccacat gcttaactga cgatcaacgc tggcaatctg tcttagcccg
     2641 cgacccgaat gccgacggcg aattcgtttt cgccgtgcgc accacgggta tcttttgccg
     2701 tccgtcttgc cgcgccagac atgccttgcg ggaaaatgtc tccttctacg caaatgccag
     2761 cgaggcactc gccgccggat ttcgcccctg caaacgttgt cagccagaca aagccaatcc
     2821 ccggcaacat cggttggata aaatcaccca cgcgtgtcga ctgctggaac aggaaacgcc
     2881 tgtaacgctg gaagccttag ccgaccaggt ggcgatgagt ccatttcatc tacatcggtt
     2941 gtttaaagcg actaccggaa tgacgcctaa agcctggcaa caggcctggc gcgctcgccg
     3001 tttgcgcgaa tcgctggcga aaggggagag cgtgacgacg tctattctta acgccggatt
     3061 ccccgacagc agcagttact accgcaaagc cgatgaaacg ctgggcatga cggctaaaca
     3121 attccgtcac ggtggcgaaa atctggcggt gcgttacgcg ctggctgatt gtgagctggg
     3181 tcgttgcctg gtggcagaaa gcgagcgggg gatttgcgcg atattgctgg gcgatgatga
     3241 cgcgacatta atcagcgagt tgcagcagat gtttcccgct gccgacaacg cgcctgccga
     3301 tctgacgttt cagcaacatg tgcgtgaagt gatcgccagc ctcaatcaac gcgatacgcc
     3361 gctgacgtta ccgctggaca ttcgcggcac tgcttttcag caacaagtct ggcaggcact
     3421 gcgcacgata ccttgtggtg aaaccgtcag ttatcagcaa ctggctaacg ccatcggcaa
     3481 accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg ctatcgtaat
     3541 accctgtcat cgagtggtcc gtggtgatgg cacactttcc ggttaccgct ggggcgtgtc
     3601 gcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat gttggatctg
     3661 tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat tttacggcgt
     3721 tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc cagccagtcg
     3781 ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat gaccaactgt
     3841 gggcatctgg gctggacgac ccatcggcaa ggttatctct attcgcccat tgatccgcaa
     3901 acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca acgtgcggct
     3961 acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg ctacgctcct
     4021 ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc gccgattgtt
     4081 tctgtttctc ttggcttacc cgcgattttt caatttggtg gcctgaaacg taatgatccg
     4141 ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga atcgcggctg
     4201 ttttatcacg gcattcaacc gctgaaagcg gggtttcatc cactcaccgg cgactgccgt
     4261 tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt attattgctg
     4321 tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt ctgcatggaa
     4381 cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat ggagctaagc
     4441 ctcgccagtg cggctttagg cattggctta attgctttta tcaatcagcg ccttatcgaa
     4501 acggcggata ccagtctgct ggtgttgccg gagtttctgg gattattgct gctgttgatg
     4561 gcaatcactc tcggatcgca actggcgctc accactttgg ggcatcactt cgtttaccga
     4621 ctgcgtagtg aatttatcaa gcggattctc gatacccacg tcgagcgcat tgaacaactc
     4681 ggtagcgcct cgttgctggc gggattaacc agcgatgtgc gcaatatcac cattgctttt
     4741 gtgcgtctgc cggaactggt gcaggggatc attctcacta tcggttcagc ggcgtatctg
     4801 tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcggt caccatctgg
     4861 ggcggttttg tgctggtggc gcgggtgtac aaacatatgg cgaccctgcg tgaaaccgaa
     4921 gataagctgt acacggattt tcaaaccgta ctcgaagggc gcaaagagct gactctgaac
     4981 cgtgaacgcg ccgagtatgt gtttaacaac ctctacattc ctgatgcgca agagtatcgc
     5041 catcatatta tccgcgcaga caccttccat cttagtgccg tgaactggtc aaacatcatg
     5101 atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gcctcggttg ggctgatacc
     5161 aacgttgctg cgacctattc gttgacgctg ttattcctgc gtacgccgct gctttcggcg
     5221 gttggcgcat tgccgacgct gctgacggcg caggtggcgt ttaacaagct gaacaaattc
     5281 gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa ctggcaaacg
     5341 ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt tgggccgatt
     5401 aacctcacca ttaaacgtgg cgagctgctg tttctgattg gtggcaacgg tagtggaaaa
     5461 tcgacgctgg cgatgttgct gacgggcttg taccagccac aaagcggcga aatcttgctg
     5521 gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt ttcggcagtg
     5581 tttaccgatg tctggctgtt tgatcaactg ctggggccgg aggggcaacc cgctaacccg
     5641 caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga gttaagcaac
     5701 gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc gctgttgctg
     5761 gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga tcaggatcca
     5821 cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat gggtaaaact
     5881 attttcgcca tcagtcatga tgatcattac tttatccacg ccgaccgcct gctggaaatg
     5941 cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc gcgtgatgcc
     6001 gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg ccaggtcaat
     6061 attctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat tataatgttt
     6121 ctaaaattag aatataattt ataaacatta tttaaatgtt gttactttag tgttaccgtt
     6181 gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta acaagatgaa
     6241 aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt cgatggcggc
     6301 aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg gcggcattat
     6361 gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga tgaccatggt
     6421 ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaacaacg ccggaaccgg
     6481 gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca gtatcagtat
     6541 tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct gggcgcacca
     6601 ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc cgcatatgag
     6661 ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg cgttgcaaca
     6721 aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca aagagtgggc
     6781 accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc gtacggaaat
     6841 tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct tgcagaagaa
     6901 atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta atgacgataa
     6961 cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca ttcgtgccaa
     7021 atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat cggggattcc
     7081 ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt cggaaaaccc
     7141 ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg gcgcaccacc
     7201 gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag tgctgtttgg
     7261 gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg atctgatgag
     7321 ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata atttcgatct
     7381 ggtgaaatac ttggtgagtc aggtgatgtt gagtgaagag gatcgttttg aagcgttgaa
     7441 agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg ggcagcgtgt
     7501 gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta ctgaagtggt
     7561 cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg cgtcaaccgc
     7621 tgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt ccagcccgca
     7681 atggcaggct acgttgaaag cgatcgttcc atcttatgga cgcaagctga acggtgatgt
     7741 agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctgggcctga aatacgacaa
     7801 gccgcaggca gcagatagta cgccgaaacc gcagttgaaa ccgcaacccg ttcaaaaaga
     7861 agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc ggatgtggcg
     7921 taaccgcctt atccgaccta cggggtggag cacttcacag cggtgtaggc ctgataagac
     7981 gcgtcagcgt cgcatcaggc aaccggctcg ggcgttagat gtcggatgcg gcgtaaccgc
     8041 cttatccaac ctacggggtg gagcacttca cagcggtgta ggcctgataa gacgcgccag
     8101 cgtcgcatca ggcaaccggc ttgggcgtta gatgtcggat gcggcgtgac cgccttatcc
     8161 gacctacggg gtgacgcact tcacagcagt ttagcgaact accgcgttgt caattttctc
     8221 ttccgccttc cagacgcggt acttcacatc tacattgtct ggcgtataca ccacgatcgg
     8281 cagcttgctg ttgtaacgca gcattccagc atcgcccaga tacgcggtga caaatttctt
     8341 ctctttcttg ccatccgggc aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa
     8401 gacataataa tcatagcccc agccttccag cgttttgttt tccagcttcc cgccgagacg
     8461 atgcaaattg caatcgactt ccagcgtctg accggttaac agttctactt tcagggtaga
     8521 ttcatcttct tgcggggtta actgaatcac ctgacgcttc atcccttttt cagcttgtgg
     8581 ataaggcgcg attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtggc
     8641 gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat
     8701 ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat ttattaattt
     8761 gctttatgaa taacttaaag aacattcaga caattttacc tgctctttta gtgttaaatt
     8821 ctgatgaggg agcaaaccag cagaaatatc tctggtgaat taatcgctat ataaatatgt
     8881 ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc acgcttcccg ccactctttt
     8941 gatcctgcta caggttttac cccgatcggg gtatgcatct ttgacacatc ctttaatatc
     9001 ttagcggcta taaaaatggc ttattaatta tgcggtttat ttggtcgctc tcaattttca
     9061 gagcgcgtta atgatggaag gtcaatgtga agattgatgc atcccgtcgg ggcatactca
     9121 ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc ctggtccggt gatgaatctc
     9181 attttctgac ccattgcacc cgttgcgacg cctgtattaa tgcttgtgaa aacaacattc
     9241 tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa aaataacgag tgcagcttct
     9301 gttacgcctg cgcgcaggct tgccccgaat cgctattttc tccgcgccac accagggcct
     9361 gggatttgca gttcaccatt ggggacgcgt gcctggcgta tcagtcagtt gaatgccgcc
     9421 gctgtcagga tagctgtgag ccaatggcaa ttatctttcg cccgacgctc tccgggatct
     9481 accagccgca acttaatagc caactttgca acggatgtgg agcatgtgca gccagctgtc
     9541 cggtatcagc cataactgcg gagtatctcc atgcacacta actggcaagt ttgcagcctg
     9601 gtcgtgcagg ccaaaagcga acgaatttca gacatcagca cccaactgaa cgcctttccc
     9661 ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt tgattgtggt ggtggaagca
     9721 gaagacagcg aaacgctgat ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg
     9781 gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg aggaaacacc atgaaactca
     9841 gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc tgcggcggct gccggtctca
     9901 gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga agccattaaa tgggataaag
     9961 cgccgtgccg tttctgcggt actggttgcg gcgttctggt cggaacgcag caggggcgtg
    10021 tggtggcctg tcagggcgac ccggacgcac cggttaaccg tggcctgaac tgcattaagg
    10081 gctatttcct gcccaagatc atgtacggta aagaccgttt gacgcagccg ctgctgcgta
    10141 tgaaaaacgg taaatatgac aaagaaggcg aatttacccc aatcacctgg gatcaggcct
    10201 tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg
    10261 gtatgttcgg ttctggtcag tggactatct gggaaggtta tgccgcgtcc aagctgttta
    10321 aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg tcactgtatg gcgtcggcag
    10381 tagttggctt tatgcgtacc tttggtatgg atgagccgat gggctgctat gacgacatcg
    10441 agcaggctga cgcgtttgtg ctgtggggcg caaacatggc ggagatgcac ccgatcctct
    10501 ggtcgcgcat cactaaccgt cgtctctcta atcagaacgt caccgtggcg gtgctttcta
    10561 cctaccagca tcgtagcttc gagctggcgg ataacggcat catctttacg ccacagtctg
    10621 acctggtgat cctgaactac atcgccaact atatcattca aaacaatgcg ataaatcagg
    10681 acttcttcag caaacacgtt aacctgcgca aaggggcgac ggacatcggc tacggtttac
    10741 gtccgaccca tccgctggaa aaagcagcga agaatccagg ttctgacgcc tccgaaccga
    10801 tgagctttga agattacaaa gccttcgttg ccgagtatac gctggaaaaa accgctgaaa
    10861 tgaccggcgt gccgaaagac cagttagaac aactggcgca gctgtatgcc gatccgaaca
    10921 agaaagtcat ctcctactgg acgatgggct tcaaccagca tactcgtggc gtgtgggcta
    10981 acaacctggt ctacaacctg cacctgctga ccggcaaaat ttcccagccg ggttgcggtc
    11041 cgttctccct gaccgggcaa ccttctgcgt gtggtactgc gcgtgaagtg ggcacctttg
    11101 ctcaccgtct gcctgcggac atggtggtga ccaacgagaa acaccgtgat atctgcgaga
    11161 agaagtggaa tatcccgagc ggcaccattc cggcgaaaat cggtctgcac gcggtggcac
    11221 aagaccgtgc gctgaaagac ggcaaactga atgtttactg gaccatgtgt accaacaaca
    11281 tgcaggccgg gccgaacatc aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact
    11341 tcatcatcgt ctccgatccg tatccgacag tcagtgcgct ggccgccgac ttgatcctgc
    11401 cgaccgcaat gtgggtagag aaagagggtg cttacggtaa cgccgaacgc cgtactcagt
    11461 tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc ggatctctgg cagttagtcc
    11521 agttctcccg ccgcttcaaa actgaagaag tgtggccgga agagctgcta gcgaagaaac
    11581 cggaactgcg tggcaaaacg ctgtacgaag tgctttatgc cacaccggaa gtgagcaaat
    11641 tcccggtatc cgaactggcg gaagatcagc tgaacgatga atcccgcgag ctgggcttct
    11701 atctgcaaaa agggctgttc gaagagtacg catggtttgg tcgcggtcac ggtcacgatc
    11761 tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg ctggccggtg gtgaacggta
    11821 aagaaacgca gtggcgttac agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct
    11881 ataagttcta cggtaaaccg gatggtaaag cggtgatctt cgcgctgccg ttcgaaccgg
    11941 cggcggaagc accggatgaa gagtacgacc tgtggctctc taccggacgc gttctggagc
    12001 actggcacac tggcagtatg actcgccgtg tgccggaact gcaccgcgcc ttcccggaag
    12061 cggtcttgtt tattcacccg ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag
    12121 tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt tgaaacgcgc ggtcgtaacc
    12181 gtccgccaca gggcctggtg tacatgccgt tcttcgacgc cgcacagctg gttaacaaac
    12241 taacgctgga cgcgaccgat ccgctctcga aagagacgga cttcaagaag tgcgcagtca
    12301 aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc
    12361 gctttctgcg cgatgttgtt cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg
    12421 ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa
    12481 acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt
    12541 acgacacctt aaaactggcg acgctggctt ctggcctgtc ggcgggcacg ccatattttg
    12601 tcgcacggga tattccttgc gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccga
    12661 gtggtgcgct ggatcgtgag atcgaatcga tcgacgacgc gcggatgggg ctggcggtgc
    12721 tggtggacca ggaaaactgt ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg
    12781 aatgcccgaa aattgatgag gccatcaccc tggagctgga gcgcaacacg cgtaccggta
    12841 agcacgcccg ctttctgccg acggttcaca gcgacgcctg taccggctgc ggtaagtgcg
    12901 aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag
    12961 gggagttagg tcaccattac cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa
    13021 cgtgacgccg ggcgcgaggc gctggtgaaa aaaggctggt ggcgcagtca ccgttggctg
    13081 gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt ttttgagcgg tccgtggttt
    13141 ggtgtgtgga tcttacacgg caactacagc tgtagcctgt tattcgacac cgttccgctg
    13201 accgatccgc tgatgacgct gcaaagtctt gccagtggtc atctgcctgc cacggtggcg
    13261 ttgaccggtg cggtgataat cactgtgctc tacgccctgg caggaaagcg attattttgc
    13321 agctgggtct gcccgctgaa cccgattacc gacttagcga actggttacg caggcgcttt
    13381 gacctcaatc agtctgcgac gatccctcgc cacattcgct acgtgctgtt ggtggtcatt
    13441 ctgctgggat cagccttaac cggcacgctc atctgggaat ggatcaaccc cgtttccctg
    13501 atggggcgta gcctggtaat gggcttcggc agcggcgcgc tgcttattct cgcgctgttt
    13561 ttatttgatc tactggtcgt tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg
    13621 ctgtatggcg tgctgggtag caaaggtgtg attaccgttg cggccagcga tcggcagaaa
    13681 tgtaaccgct gtatggattg ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg
    13741 gtgctggatg aacaaagccc ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc
    13801 tgcgtggatg tctgttctga ggatgtattt acaataacta cacgatggag ttcgggagcg
    13861 aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca atggacggcg atgctggccc
    13921 tggtggtaag cggcgcggtt tgggcggcta atggcgtcga ttttagccag tcgccggaag
    13981 tatccgggac gcaggaaggg gccattcgga tgccaaaaga gcaggatcgg atgccgctga
    14041 actatgtgaa tcagccgcca atgatcccgc atagcgttga aggttatcag gtaacgacca
    14101 ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc
    14161 gtatcagtcc tactcacttt atggacagcg acggcaaagt gggcgcggaa gtggctccgc
    14221 gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga taccgcgcca atcgtgggga
    14281 atacctttac cccatcaaaa ggttacggga aataagaggt tattatggga aattctgacc
    14341 gtaagcctgg tctgattaag cgcctgtgga aatggtggcg tacccccagc cgtctggcgc
    14401 tgggaacgct gctgttgatc ggttttgtcg gcggcatcgt cttctggggc ggctttaaca
    14461 ccggaatgga aaaagccaat accgaagagt tctgcattag ctgccacgaa atgcgtaaca
    14521 cggtgtatca ggaatacatg gattccgtgc actacaacaa ccgtagcggc gtccgtgcga
    14581 cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag
    14641 caagtaaaga gctgtatggt aaaatttttg gcgttattga cacgccgcag aaatttgaag
    14701 ctcatcgtct gacaatggca cagaatgagt ggcggcgcat gaaggacaat aactcgcagg
    14761 agtgccgtaa ctgtcacaac ttcgagtata tggatacaac cgcccagaaa tcggttgccg
    14821 cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg tattgattgc cataaaggga
    14881 tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg tttttaacag ggttattgcg
    14941 tgggtatgct tgaagccaga gagttacttt gtgagcggga tgaacgaacc ttatttagtg
    15001 gcttgtcatt tacgctgaac gcaggagagt gggtacaaat caccggtagc aacggcgcgg
    15061 ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg ccctgacgca ggcgaggttc
    15121 tctggcaagg gcagcccttg catcaggtac gcgacagcta ccatcaaaac ctgttatgga
    15181 taggccatca gccggggatc aaaacccggc tgacggcgtt agaaaatctg cacttttatc
    15241 atcgcgatgg cgataccgca caatgtctgg aagccctggc gcaggccggg cttgccggat
    15301 tcgacgatat tccggtaaat caactttctg ccgggcaaca acgccgcgtc gctttagcgc
    15361 gtctgtggct gacccgtgcc acgttatgga tcctcgacga gccttttacc gcgattgacg
    15421 ttaacggcgt cgatcgtctg acccagcgta tggcgcagca tacggagcag ggggggattg
    15481 tgattctgac tacccaccag ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac
    15541 tgacgcaaac gagggccgca tgatgttctg gcgcattttc cgtcttgagc tgcgtgtagc
    15601 gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc ttcctgattg taattaccct
    15661 ttttccgctc agtatcggtc cggagccgca actgctggcg cgtattgcac cgggcattat
    15721 ctgggttgct gcgctgcttt catccttgct ggcgctggaa cgactgttcc gtgacgattt
    15781 gcaggacggc agtcttgaac aattgatgtt gttgccgtta cccttgcccg ccgttgtgct
    15841 ggcgaaggtg atggcgcact ggatggtaac cggtctgccg ttactcatcc tttcgccact
    15901 ggtagcaatg ctactgggaa tggatgttta tggctggcaa gtgatggcgc tgacgctgct
    15961 gctgggaacg cctacgcttg gctttctcgg tgcaccgggt gtggcgttga cagtgggact
    16021 taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg ctgactatcc cattactcat
    16081 ctttgccacc gccgcgatgg acgcggcttc catgcatttg cccgttgacg ggtatctggc
    16141 aattttaggc gcgttgctgg caggcaccgc gacattaagt ccttttgcga cggcggcagc
    16201 gttacgaatc agcattcaat aacgctggat tactgatttt tgttcgagtc tggtatcgaa
    16261 actatgtgga aaacactgca tcaactggcg atcccaccac ggctgtatca aatctgtggc
    16321 tggtttatac cgtggctggc aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg
    16381 ggattcggct ttgctccggc tgattatcag cagggaaata gctaccgcat tatctacctg
    16441 catgtgcctg cggcgatctg gtcgatgggc atttatgcat caatggcagt ggcagcgttt
    16501 attggccttg tctggcagat gaaaatggcc aacctggcgg tggcggcgat ggcccccatt
    16561 ggtgccgtgt ttacctttat tgccctggtt accggctctg catggggaaa accgatgtgg
    16621 ggcacctggt gggtatggga tgcacgtctg acttctgaac tggtgctgct gtttttgtat
    16681 gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca
    16741 ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta ttcattactc cgtggagtgg
    16801 tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt
    16861 tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt ctgccacgct gacgctgatg
    16921 cggatgcgta atttgatttt gctgatggaa aaacgccgtc cgtgggtgag tgaactgata
    16981 ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg
    17041 gcggttacgc cttttttgtc tggctggcgg tggtgatgac cgttattccg ctggtggttt
    17101 tggtcgtgca ctcggtgatg caacatcgcg caattctgcg tggtgtggcg caacagcggg
    17161 cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc tgcatgaata ttcgccgtaa
    17221 aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg gcgctgacta tcggtctggt
    17281 gctatatgcg ctgcgctcga atatcgatct cttttatacg ccgggggaaa ttctctacgg
    17341 caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt ttgcgcgttg gcgggatggt
    17401 gatgctgggt agtgtgcagc gcgatcccaa ttcgctgaaa gtgaccttca ctatttacga
    17461 tgctgaaggc tcagtggatg tctcttacga aggcattttg ccggatctgt tccgtgaagg
    17521 gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat catatcctcg cgaaagaagt
    17581 gctggcgaaa cacgacgaaa actacacgcc gccagaagtt gagaaagcga tggaagctaa
    17641 ccaccgtcgc ccggcgagtg tttataagga cccagcatca tgatgccgga aattggaaac
    17701 gggctgctgt gtctggcgtt agggattgcg ctgctgctgt ccgtgtatcc gctatggggc
    17761 gtggcgcgcg gagatgcgcg catgatcgcg tcttcccgct tgtttgcctg gctgctgttt
    17821 atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt tcgtggtcaa cgactttacc
    17881 gtcacctatg ttgccagcta ctccaatacc cagcttccgg tgtggtatcg cgtggcggct
    17941 acctggggcg cgcatgaagg ctcgctactg ctgtgggtgc tgctgatgag cggctggacc
    18001 tttgcggtgg cgatttttag tcagcgtatt ccgctggata ttgtggcccg cgtactggcg
    18061 ataatgggga tggtcagcgt cggctttttg ctgttcattc tctttacctc taacccattc
    18121 tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc ttaacccgct gttacaggac
    18181 ccggggctga tcttccatcc gcctctgctt tatatggggt acgtgggttt ctcggtggcg
    18241 tttgcttttg ccattgcttc tttgctgagc gggcgtctgg acagcactta tgcgcgtttt
    18301 actcgcccgt ggacgctggc ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc
    18361 gcatgggcct attacgaact cggctggggg ggctggtggt tctgggatcc ggtagaaaac
    18421 gcctcgttta tgccgtggct ggtggggact gcgctgatgc actcactggc ggtcactgaa
    18481 caacgcgcca gcttcaaagc gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt
    18541 ctgttgggga ctttcctcgt gcgttccggc gtgctggtat cggtacacgc gtttgcgtct
    18601 gatccggcgc gcggtatgtt tatcctcgcc tttatggtgc tggtgattgg aggttcgctg
    18661 ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg
    18721 cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg ccgcgatgct ggtggtgttg
    18781 ctggggacgc tgctgccgct ggtgcacaag caactgggac tgggcagtat ttcgattggc
    18841 gaaccgttct tcaacaccat gtttacctgg ctgatggtgc cgtttgcgct actgctgggt
    18901 gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta aaatccgcaa tttattgatt
    18961 atcgccttca tctcaacgct ggtgctgtct ctgctgttgc cgtggctgtt tgagagcaaa
    19021 gttgtggcga tgacggtgct cggcctggca atggcctgct ggattgcggt gctggcaatt
    19081 gcggaagctg cgctgcgtat ttcacgcggc acgaaaacca ccttcagtta ttgggggatg
    19141 gtggctgctc acctggggct ggcagtgaca attgttggca ttgcttttag ccagaactat
    19201 agcgttgagc gtgatgtgcg catgaagtcc ggcgatagca tcgatattca tgaatatcgc
    19261 ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact ggcgtggcgg tgtggcgact
    19321 atcggcgtaa cgcgagaggg taaaccggaa acagtgctgt atgcggaaaa acgttattac
    19381 aacactgccg ggtcgatgat gaccgaagcg gcgattgacg gtggcatcac gcgtgacctg
    19441 tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg ctgtgcgtct ttactacaaa
    19501 ccatttgttc gctggatttg ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt
    19561 ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa aaactgcgcc ggaggctgta
    19621 tgaagcgcaa agtattgtta attccgttga ttatcttcct ggcgattgcc gcggcgctgc
    19681 tgtggcagct ggcgcgtaat gccgaagggg atgatccgac caatctggaa tcggcgctca
    19741 ttggcaagcc agtgccgaag tttcgcctcg aatcactgga caatccgggg cagttttatc
    19801 aggcggatgt gctgactcag ggcaaaccag tgctgcttaa cgtctgggcg acctggtgtc
    19861 cgacctgccg tgcggaacat caatatctga atcagctttc tgcgcagggc atccgcgtag
    19921 tcggcatgaa ctataaagac gatcgccaga aggcaatcag ctggctgaaa gagctgggca
    19981 atccttacgc gctaagcctg tttgatggcg atggcatgtt agggctggat ctcggtgtct
    20041 atggcgcgcc agaaacgttc cttattgacg gcaacggcat cattcgctat cgccatgcgg
    20101 gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc gctgtgggag aaatacagta
    20161 aggaggccgc acaatgaggt ttttattggg catgctgatg ctgatgatct ccggctcagc
    20221 gctggcgacc atagacgtgt tgcagtttaa agatgaagca caggaacaac agttccgtca
    20281 gctcactgaa gaactgcgct gcccgaaatg ccagaacaac agcattgccg attccaactc
    20341 gatgattgcc accgacctgc gccagaaagt gtatgaactg atgcaggaag gtaaaagtaa
    20401 aaaagagatt gtcgattata tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc
    20461 gttaacgccg ctgaccgtgc tgctgtgggt gctgccagta gtagctattg gcattggcgg
    20521 ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgtg gtgccggaag cgtttcctga
    20581 acaaagcgtg ccggaaggta agcgtgccgg atatgtcgct tatttgccgg gcattgtggt
    20641 ggcgttaatt gtggctggcg tcagctacta ccagaccgga aattatcaac aggtgaaaat
    20701 ctggcagcag gccacggcac aggctccggc gttgctggac agggcgctgg atccgaaagc
    20761 tgatccgctc aacgaagaag agatgtcgcg ccttgcgctg gggatgcgta ctcaactgca
    20821 aaaaaatccg ggagatatag acggctggat tatgttgggc cgcgttggca tggcgctggg
    20881 taacgccagt atcgccaccg atgcatacgc tactgcgtat cgcctcgatc cgaaaaacag
    20941 tgatgccgca ctgggttatg ctgaagcgtt gacacgttca tctgatccca acgacaaccg
    21001 cctcggcggc gaactgctac gtcagctggt gagaacggac catagcaata tccgtgtgct
    21061 aagcatgtat gcgtttaatg cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg
    21121 ggagatgatg ttgaaactct tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag
    21181 tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt aaataaaaag aggcccgatg
    21241 gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt gcaggaacag aatggtggcc
    21301 gccacgcgtg agcggacatt gagtttacgc agcagatttc gaatatgaac ttttactgtc
    21361 tgctcggaaa tattcaacac agaggcaatc tgtttatttg acagcccctg tgccagctcg
    21421 tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg gatcttcttc cgcgccaaac
    21481 atttcacgtt cacgtaagta ctgattgacg cgttcgctaa agactttgct gcctttcgct
    21541 ccggcacgaa tcgcttccag caacacttcc gggtcgctgt ctttcagcag ataaccgtct
    21601 gcgcctgcgt ctatcagcgc aaagacatcg ctggaggcat cggatacggt caggataata
    21661 atttgcgcgg taacgccatc cctgcgcaag gcattgaggg tatccaggcc actcatacct
    21721 ttcatattga gatccagcaa gatcacgtcg atatccagtc tattcgccag atcgatcgcg
    21781 ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag gatcaagctc cagtaactga
    21841 cgaacaccgc gtcgcataag tggatgatca tccacaatca tcacctgaaa aggcgttgct
    21901 tcaggcatag tagtctcctg aggttttatt agaatcatta tgtttgagcg tagcgttggt
    21961 aacgctacca ggcgcgtgaa tgttacccca ttttatgtat ggacatgtag tgaaaactac
    22021 aaccttgccg ttcgctttgg catcattttc attataaaga cggaattttg catcagcttc
    22081 tcatcaagaa aaacaactaa aaaatcaatc acaatacttg tgcatttctt taaacagttt
    22141 ttaaaaactg actccgggta tggagctatg ggtattttct gtacccaatg cttttaacag
    22201 caattaattt cataggatga aagctcaatg catcaatctg gttctgtttc tctttgtcgt
    22261 tccgcaatat ctgttctggt ggctacagcg ttatattcac ccatagcatt ggcatcaaca
    22321 gttgagtatg gtgagacagt tgatggtgtt ggcctggaaa aagatatcca gctggtttat
    22381 gggaccgcca ataatacgaa aatcaatcct ggcggagaac agcatataaa agaatttggt
    22441 gtaagtagta atactgaaat taacggcggg tatcagtaca ttgaaatgaa tggcactgca
    22501 gaatactcag tattaaatga tggttatcaa attgttcaaa tgggtggcgc ggtaaaccag
    22561 actacgctca ataatggagt gctacaggtt tatgg
//
LOCUS       DAEEBB010000062        22139 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEEBB010000062 DAEEBB010000000
VERSION     DAEEBB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.062"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..524)
                     /locus_tag="KLF07_002236"
     CDS             complement(<1..524)
                     /locus_tag="KLF07_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD9832244.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="KLF07_002237"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="KLF07_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBD9832245.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1941..2603
                     /locus_tag="KLF07_002238"
     CDS             1941..2603
                     /locus_tag="KLF07_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD9832246.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="KLF07_002239"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="KLF07_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBD9832247.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /locus_tag="KLF07_002240"
     CDS             3137..3505
                     /locus_tag="KLF07_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBD9832248.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="KLF07_002241"
     CDS             3543..3848
                     /locus_tag="KLF07_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBD9832249.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="KLF07_002242"
     CDS             3851..4255
                     /locus_tag="KLF07_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBD9832250.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="KLF07_002243"
     CDS             4245..4544
                     /locus_tag="KLF07_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBD9832251.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4730..5122
                     /locus_tag="KLF07_002244"
     CDS             4730..5122
                     /locus_tag="KLF07_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD9832252.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5192..6205
                     /gene="yqjG"
                     /locus_tag="KLF07_002245"
     CDS             5192..6205
                     /gene="yqjG"
                     /locus_tag="KLF07_002245"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBD9832253.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6470..6835
                     /locus_tag="KLF07_002246"
     CDS             6470..6835
                     /locus_tag="KLF07_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD9832254.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="KLF07_002247"
     CDS             7077..7433
                     /locus_tag="KLF07_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD9832255.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="KLF07_002248"
                     /pseudo
     CDS             complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="KLF07_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8485..9186
                     /locus_tag="KLF07_002249"
     CDS             8485..9186
                     /locus_tag="KLF07_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBD9832256.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /locus_tag="KLF07_002250"
     CDS             9209..9373
                     /locus_tag="KLF07_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832257.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /locus_tag="KLF07_002251"
     CDS             complement(9507..10817)
                     /locus_tag="KLF07_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBD9832258.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="KLF07_002252"
     CDS             complement(10845..12176)
                     /locus_tag="KLF07_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBD9832259.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="KLF07_002253"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="KLF07_002253"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD9832260.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="KLF07_002254"
     CDS             complement(13887..14276)
                     /locus_tag="KLF07_002254"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBD9832261.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="KLF07_002255"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="KLF07_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBD9832262.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="KLF07_002256"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="KLF07_002256"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBD9832263.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="KLF07_002257"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="KLF07_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBD9832264.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="KLF07_002258"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="KLF07_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBD9832265.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="KLF07_002259"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="KLF07_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBD9832266.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21420..21764
                     /gene="tdcR"
                     /locus_tag="KLF07_002260"
     CDS             21420..21764
                     /gene="tdcR"
                     /locus_tag="KLF07_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBD9832267.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5805 a         5650 c         5169 g         5515 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaagc gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacacgcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaatt
//
LOCUS       DAEEBB010000063        21643 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEEBB010000063 DAEEBB010000000
VERSION     DAEEBB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.063"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="KLF07_002261"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="KLF07_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBD9832268.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="KLF07_002262"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="KLF07_002262"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD9832269.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="KLF07_002263"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="KLF07_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBD9832270.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="KLF07_002264"
     CDS             complement(3717..4511)
                     /locus_tag="KLF07_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBD9832271.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="KLF07_002265"
     CDS             4649..4990
                     /locus_tag="KLF07_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD9832272.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="KLF07_002266"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="KLF07_002266"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBD9832273.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="KLF07_002267"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="KLF07_002267"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBD9832274.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="KLF07_002268"
     CDS             8906..10198
                     /locus_tag="KLF07_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD9832275.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="KLF07_002269"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="KLF07_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBD9832276.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="KLF07_002270"
     CDS             complement(10731..11189)
                     /locus_tag="KLF07_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBD9832277.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="KLF07_002271"
     CDS             complement(11186..12103)
                     /locus_tag="KLF07_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD9832278.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="KLF07_002272"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="KLF07_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBD9832279.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="KLF07_002273"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="KLF07_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBD9832280.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="KLF07_002274"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="KLF07_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBD9832281.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="KLF07_002275"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="KLF07_002275"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD9832282.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="KLF07_002276"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="KLF07_002276"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBD9832283.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="KLF07_002277"
     CDS             16063..16749
                     /locus_tag="KLF07_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9832284.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="KLF07_002278"
     CDS             16746..19160
                     /locus_tag="KLF07_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9832285.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="KLF07_002279"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="KLF07_002279"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBD9832286.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="KLF07_002280"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="KLF07_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5684 c         5800 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccgcgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAEEBB010000064        21551 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEEBB010000064 DAEEBB010000000
VERSION     DAEEBB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.064"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..1363
                     /gene="espL1"
                     /locus_tag="KLF07_002281"
                     /pseudo
     CDS             1..1363
                     /gene="espL1"
                     /locus_tag="KLF07_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1535..1642
                     /locus_tag="KLF07_002282"
     CDS             1535..1642
                     /locus_tag="KLF07_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832287.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1695..2453)
                     /locus_tag="KLF07_002283"
     CDS             complement(1695..2453)
                     /locus_tag="KLF07_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBD9832288.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2740..3669
                     /gene="pfkB"
                     /locus_tag="KLF07_002284"
     CDS             2740..3669
                     /gene="pfkB"
                     /locus_tag="KLF07_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBD9832289.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3770..4060
                     /gene="ghoS"
                     /locus_tag="KLF07_002285"
     CDS             3770..4060
                     /gene="ghoS"
                     /locus_tag="KLF07_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBD9832290.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4166..5026
                     /locus_tag="KLF07_002286"
     CDS             4166..5026
                     /locus_tag="KLF07_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBD9832291.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQSELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5067..5603)
                     /locus_tag="KLF07_002287"
     CDS             complement(5067..5603)
                     /locus_tag="KLF07_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBD9832292.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5750..6418
                     /gene="hxpB"
                     /locus_tag="KLF07_002288"
     CDS             5750..6418
                     /gene="hxpB"
                     /locus_tag="KLF07_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBD9832293.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6581..7171
                     /locus_tag="KLF07_002289"
     CDS             6581..7171
                     /locus_tag="KLF07_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD9832294.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7304..8695
                     /gene="tcyP"
                     /locus_tag="KLF07_002290"
     CDS             7304..8695
                     /gene="tcyP"
                     /locus_tag="KLF07_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBD9832295.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8699..9515)
                     /locus_tag="KLF07_002291"
                     /pseudo
     CDS             complement(8699..9515)
                     /locus_tag="KLF07_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9792..10055)
                     /gene="cedA"
                     /locus_tag="KLF07_002292"
     CDS             complement(9792..10055)
                     /gene="cedA"
                     /locus_tag="KLF07_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBD9832296.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10238..12499
                     /gene="katE"
                     /locus_tag="KLF07_002293"
     CDS             10238..12499
                     /gene="katE"
                     /locus_tag="KLF07_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBD9832297.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12757..13515)
                     /gene="chbG"
                     /locus_tag="KLF07_002294"
     CDS             complement(12757..13515)
                     /gene="chbG"
                     /locus_tag="KLF07_002294"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBD9832298.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13528..14880)
                     /gene="celF"
                     /locus_tag="KLF07_002295"
                     /gene_synonym="chbF"
     CDS             complement(13528..14880)
                     /gene="celF"
                     /locus_tag="KLF07_002295"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBD9832299.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14985..15827)
                     /gene="chbR"
                     /locus_tag="KLF07_002296"
     CDS             complement(14985..15827)
                     /gene="chbR"
                     /locus_tag="KLF07_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBD9832300.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15835..16185)
                     /gene="chbA"
                     /locus_tag="KLF07_002297"
     CDS             complement(15835..16185)
                     /gene="chbA"
                     /locus_tag="KLF07_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBD9832301.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16236..17594)
                     /gene="chbC"
                     /locus_tag="KLF07_002298"
     CDS             complement(16236..17594)
                     /gene="chbC"
                     /locus_tag="KLF07_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBD9832302.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17679..17999)
                     /gene="chbB"
                     /locus_tag="KLF07_002299"
     CDS             complement(17679..17999)
                     /gene="chbB"
                     /locus_tag="KLF07_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBD9832303.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18298..18636)
                     /gene="osmE"
                     /locus_tag="KLF07_002300"
     CDS             complement(18298..18636)
                     /gene="osmE"
                     /locus_tag="KLF07_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBD9832304.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18838..19665
                     /gene="nadE"
                     /locus_tag="KLF07_002301"
     CDS             18838..19665
                     /gene="nadE"
                     /locus_tag="KLF07_002301"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBD9832305.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19895..20782
                     /gene="cho"
                     /locus_tag="KLF07_002302"
     CDS             19895..20782
                     /gene="cho"
                     /locus_tag="KLF07_002302"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBD9832306.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20742..21317)
                     /gene="ves"
                     /locus_tag="KLF07_002303"
     CDS             complement(20742..21317)
                     /gene="ves"
                     /locus_tag="KLF07_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBD9832307.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5738 a         5313 c         4938 g         5562 t
ORIGIN      
        1 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
       61 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
      121 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
      181 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
      241 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
      301 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
      361 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
      421 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
      481 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
      541 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
      601 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
      661 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
      721 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
      781 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
      841 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
      901 aacaaaaaaa atggtgatga gtattttagc catgaaaata catgatggtg agccaggatt
      961 atacgccgca atggaaaata atcacccttt gtgtgtcaca cggttcctct ctaaaattaa
     1021 tggcatcgcc tttaaataca agttgagcaa agctaacatc atggatttat taaaaggagc
     1081 tacagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg aagacgtcgt
     1141 gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta gtcaacatca
     1201 gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc atatagccat
     1261 tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg tcatcagcca
     1321 aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta tttacgatat
     1381 atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca tgtgcacaac
     1441 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tatccctttt gttacactcc
     1501 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
     1561 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
     1621 ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg cataacacgg
     1681 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
     1741 tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
     1801 aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt atcttccgcg
     1861 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
     1921 ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
     1981 acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca
     2041 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
     2101 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
     2161 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
     2221 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
     2281 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
     2341 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
     2401 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
     2461 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
     2521 attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaataactta gcaatgtatt
     2581 cttatttcat tttttgaata agcatgtggc gaaaacagat ttttatttat atatatttat
     2641 ctgcaaaatt ttaaataaag ctccaataaa tcatattgtt aatttcttca ctttccgctg
     2701 attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat ctatacgttg
     2761 acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc cgaaggaaaa
     2821 ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt cgcccgcgcc
     2881 attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc gaccggcgaa
     2941 cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga agccaaagac
     3001 tggacccggc agaatttaca tgtacatgtg gaagcaagcg gtgagcagta tcgttttgtt
     3061 atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca agttctggaa
     3121 attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt gaagctggaa
     3181 aaattaaccc aactgatttc cgcttcgcaa aaacaaggga tccgctgcat cgtcgacagt
     3241 tctggcgaag cgttaagtgc agcactggca attggtaaca tcgagttggt taagcctaac
     3301 caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccggacga tgtccgcaaa
     3361 gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc ccttggtcca
     3421 caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc accggtgaaa
     3481 agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact gaaactggca
     3541 gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag tgcagccaca
     3601 ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta cgcttacctt
     3661 tcccgctaac aaaaacattc ccccagcatt gggggaatca ccaccaacct gtcggcaacg
     3721 cgtttctccg actttgctca aaagtcatgt gataacaaag gggtgaacta tggccagtgg
     3781 cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga ctgaaattaa
     3841 cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg atgatgaggg
     3901 aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa gtgaagaaga
     3961 aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc ctgaaatcac
     4021 catcacgact tgggaggaat ggaatagcaa cagaaaataa atggtttttg ggcaataatc
     4081 agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa ccttatgatc
     4141 tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt tgagcgagca
     4201 gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag tccatgccgc
     4261 atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa gggaactgct
     4321 tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta aaaccgtcac
     4381 cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg tgatggatta
     4441 tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc aaattgcgcg
     4501 tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact cgctctccac
     4561 aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg aacaacggat
     4621 tggctggcag tcggaactgg cagcagagaa agggatcgct ttcggcaata tcgacgccat
     4681 cgtcgagcat attcagcagc gtctggcctc gcatcaactg cagccttctc tgttgcacgg
     4741 cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt tcgaccctgc
     4801 ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata ctgaacagcc
     4861 gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt tccttgaacg
     4921 tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg gcggtcagca
     4981 tttggttatt gctcagcagt cattggatag attattagca gcatgatatg ggttgaggat
     5041 aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa gaacacataa
     5101 cctgcagcaa tacaaataac cggcaggata tatagtgaga aaaactgcag gaaaatagta
     5161 tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc ttcccctttg
     5221 gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc ctggcgactc
     5281 atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa aatcacccag
     5341 aacataacgt tcacgccacc attaaaatta ggggtcgggg agttatacca gaacagattc
     5401 aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc gagcatgacc
     5461 gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa agaaatcaat
     5521 gtggagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa aacagcaatg
     5581 cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt ttcacttagt
     5641 ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg cgcttaatca
     5701 taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa tgtcaacccc
     5761 gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact cagaaccttt
     5821 acgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct cccgtcgtaa
     5881 cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt ggtacgcccg
     5941 gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg cccgtgccat
     6001 ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagctg ttgcgttatg
     6061 caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata tgctggaaaa
     6121 agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg ctgaaaaact
     6181 gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac tgggcgttga
     6241 cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt ctaaagcagc
     6301 ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac gttttgtatt
     6361 agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc tcggttaatg
     6421 accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt caactgacgg
     6481 attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca ctgtataaaa
     6541 atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg aaggtcacct
     6601 tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc ccgtgctggc
     6661 acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt taccggacat
     6721 cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac cgatcgcccg
     6781 cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc tgctggcaac
     6841 cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac aaggaatggt
     6901 gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg ttcccctgct
     6961 ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg gcaaccaact
     7021 ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc attcattacc
     7081 cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga tccagtttca
     7141 tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt ctggaataag
     7201 caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc caacactatg
     7261 actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact ttccattaat
     7321 tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc gccataaaca
     7381 gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt ttggccttgc
     7441 cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac agtggtttaa
     7501 catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt tagtcttcgc
     7561 ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca aaatcagttt
     7621 tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg gtgtgctggt
     7681 caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag aaactgcacg
     7741 tctgaacgcc attgaaagta actatgttgg taaagtctct gatctgagcg ttccgcagct
     7801 ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca atccgacgtc
     7861 aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga aactgctgaa
     7921 ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac aaagctgggt
     7981 gatgaaactg gttcgccttg tcatgcagtt gaccccttac ggcgttctgg ctctaatgac
     8041 caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt tcgttgtcgc
     8101 gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg gcattaatgg
     8161 cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg cctttaccag
     8221 ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc gtctgggcgt
     8281 tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc agaacggctg
     8341 cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg gcattaaccc
     8401 gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt ccgcaggcgt
     8461 tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg cgatgggcct
     8521 gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca tgggccgtac
     8581 ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt ggctgaagca
     8641 aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc gttaattctt
     8701 atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa tgttccatcg
     8761 cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg tccaacttat
     8821 caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc tcatacccga
     8881 gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca ccacttccca
     8941 ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat attatcgaag
     9001 gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc ggcaggatgt
     9061 atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct attttttata
     9121 ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat cccaacaaaa
     9181 ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat tatcactcta
     9241 tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggagctcc tggctttgag
     9301 gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga ctctgctatt
     9361 tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat ggtagactca
     9421 agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc ataattactc
     9481 tgtattaagt ttgtagaaaa catctcccgc cccatattgt taacaaaatt attatctcat
     9541 ttaaatctaa gtcacttaca atataagttt aagagcgacg ccacaggatg aactatcaaa
     9601 aatagctcat catgattagc aaaacttaac cattttaaaa taaataaaca attaaagaaa
     9661 aaagatcact tatttatagc aatagctcgt caaaggcagc tttttgttac aggtggtttg
     9721 aatgaatgta gcaacgaaat gcagaatttc aggtcatgta actcccggca aaaccgggaa
     9781 gtatgtaatc cttactcagt cacttcccct tcctggcgga tctgatttgc ccaacgttgg
     9841 gcagattcag gcacagtaaa cgccggtgag cgcagaaatg actctcccat cagtacaaac
     9901 gcaacatatt tgccacgcag catccagaca tcacgaaacg aatccatctt tatcgcatgt
     9961 tctggcggcg cgggttccgt gcgtgggaca tagctaataa tctggcggtt ttgctggcgg
    10021 agcggtttct tcattactgg cttcactaaa cgcatattaa aaattagaaa aactgtagtt
    10081 tagccgattt agcccctgta cgtcccgctt tgcgtgtatt tcataacacc gtttccagaa
    10141 tagtctccga agcgggatct ggctggtggt ctatagttag agagtttttt gaccaaaaca
    10201 gcggcccttt cagtaataaa ttaaggagac gagttcaatg tcgcaacata acgaaaagaa
    10261 cctacatcag caccagtcac cactacacga ttccagcgaa gcgaaaccgg ggatggactc
    10321 actggcacct gaggacggat ctcatcgtcc agcggctgaa ccaacaccgc caggtgcaca
    10381 acctaccgcc cctgggagcc tgaaagcccc tgatacacgt aacgaaaaac ttaattctct
    10441 ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg gcgtgcgcat
    10501 cgccgatgat caaaactcac tgcgtgccgg taaccgtggt ccaacgctgc tggaagattt
    10561 tattctgcgc gagaaaatca cccactttga ccatgagcgc atcccggaac gtattgttca
    10621 tgcacgcgga tcagcagctc acggttattt ccagccatat aaaagcttaa gcgatatcac
    10681 caaagcggat ttcctctcag atccgaacaa aatcacccca gtatttgtta ggttctctac
    10741 cgttcagggt ggcgctggct ctgcagatac cgtgcgtgat atccgtggtt ttgccaccaa
    10801 gttctatact gaagagggta tttttgacct cgttggcaat aacacgccaa tcttctttat
    10861 ccaggatgcg cataaattcc ccgattttgt tcatgcggta aaaccagaac cgcactgggc
    10921 aattccacaa gggcaaagtg cccacgatac tttctgggat tatgtttctc tgcaacctga
    10981 aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca gttaccgcac
    11041 catggaaggc ttcggtattc acaccttccg cctgattaat gccgaaggga aggcaacgtt
    11101 tgtacgtttc cactggaaac cactggcagg taaagcctca ctcgtttggg atgaagcaca
    11161 aaaactcacc ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag ccattgaagc
    11221 aggcgatttt ccggaatacg aactgggctt ccagttgatt cctgaagaag atgaattcaa
    11281 gttcgacttc gatcttctcg atccaaccaa actgatcccg gaagaactgg tgcccgttca
    11341 gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac ttctttgccg aaaacgaaca
    11401 agcagcattc catccaggtc atattgttcc tggtctggac ttcaccaacg atccgctgtt
    11461 gcagggacgt ttgttctcct ataccgatac acaaatcagt cgtcttggtg ggccgaattt
    11521 ccatgagatt ccgattaacc gtccgacctg cccttaccac aacttccagc gtgatggcat
    11581 gcatcgtatg gggatcgaca ctaacccggc gaattacgaa ccgaactcga tcaacgataa
    11641 ctggccgcgc gaaacaccgc cgggaccgaa acgcggcggt tttgaatcat atcaggaacg
    11701 cgtggaagga aataaagttc gtgagcgcag tccatcgttt ggcgaatatt attcccatcc
    11761 gcgtctgttc tggctaagtc agacgccatt cgagcagcgc catattgtcg atggtttcag
    11821 ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg accagctggc
    11881 gcatattgat ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg aactgactga
    11941 cgaccagctg aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg atccatcctt
    12001 aagtttgtac gccattcctg acggtgatgt gaaaggtcgc gtggtagcga ttttacttaa
    12061 tgatgaagtg agatcggcag accttctggc cattctcaag gccctgaagg ccaaaggcgt
    12121 tcatgccaaa ctgctctact cccgaatggg tgaagtgact gcggatgacg gtacggtgtt
    12181 gcctatagcc gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg tcattgtccc
    12241 ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc aactactacc tgatggaagc
    12301 ctacaaacac cttaaaccga ttgcgctggc gggagacgcg cgcaagttta aagcaacaat
    12361 caaggtcgct gaccagggtg aagaagggat tgtggaagct gacagcgctg acggtagttt
    12421 tatggatgaa ctgctaacgc tgatggcagc acaccgcgtg tggtcacgca ttcctaagat
    12481 tgacaaaatt cctgcctgat gggaggcggc gcaattgcgc cgcctcaaat gatttacata
    12541 gtgcgctttg tttatgccgg atgcggcgtg aacgccttat ccggcctaca aaactgtgca
    12601 aattcaatat attgcaggaa acacgtaggc ctgataagcg aagcgcatca ggcagttttg
    12661 cgtttgtcag cagtctcaag cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga
    12721 tgcaaggttc acgccgcatc tggcaaacat cctcacttac acatcccgat aactccccaa
    12781 ccgataacca cgctgagcga tagcaccttt caacgacgct gatgtcaaca catccagctc
    12841 cgttaagcgt gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg
    12901 acacatcacc tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa
    12961 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag
    13021 attagccggt aaatcaccag agttagatag cggctgacgg tcgatgcgca acgcaatccc
    13081 ctcttcagcc gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg
    13141 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
    13201 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
    13261 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
    13321 agtaaggaca aagtacatcc ctatggccag actcggttca tcacgactca actgcaccgc
    13381 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
    13441 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
    13501 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
    13561 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
    13621 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
    13681 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
    13741 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
    13801 cttacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
    13861 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
    13921 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
    13981 cgggttttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc
    14041 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata
    14101 aagcaaataa gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag
    14161 atcgaaaata tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa
    14221 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
    14281 gccgaacaaa tcgatagata aatcatcgct gtctttcagc atcagaacat cgtgaataaa
    14341 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
    14401 ataaacggct tcagtaacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
    14461 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
    14521 aaacagaccg cccgcgccgt tggtttcctg gccaagataa ccatgactta atggaatacg
    14581 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
    14641 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
    14701 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
    14761 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
    14821 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
    14881 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
    14941 aatccagcaa caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca
    15001 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
    15061 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
    15121 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
    15181 ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc
    15241 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
    15301 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
    15361 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
    15421 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
    15481 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
    15541 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
    15601 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
    15661 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
    15721 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
    15781 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
    15841 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
    15901 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
    15961 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
    16021 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
    16081 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
    16141 ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc atcatacatc gttcctctct
    16201 attcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc gttagcgata
    16261 tcttcttcgc gctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag
    16321 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg caaccagcaa tgcggcgacg
    16381 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
    16441 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
    16501 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
    16561 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa
    16621 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
    16681 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
    16741 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
    16801 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
    16861 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
    16921 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
    16981 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
    17041 cgcgatactg aagcaggtac gctatcgggc aatttaatga cccaattgcg gcggacaata
    17101 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgctcca
    17161 cctaaccagt ttgcgccaac cacataggcc tcaccgacac tatatggggt gacggtcata
    17221 aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc
    17281 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
    17341 ttgccgccaa tacctttcag gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata
    17401 cctaaggaat aaaaaaacgc cccctcccca aagcttagaa aaacgttgtt aattaataca
    17461 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
    17521 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca
    17581 ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg cggtattgat
    17641 ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta
    17701 atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag
    17761 tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca
    17821 atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc
    17881 ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat
    17941 accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata
    18001 aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc
    18061 catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg
    18121 gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg
    18181 ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca
    18241 aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta
    18301 cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac
    18361 atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag
    18421 gatgtaggtc tggcaagtac cacgagcatg gatcatgctc acttcagacg aaggtttacc
    18481 cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg
    18541 tacaaactgg tctttagtac gatcataagc cgtacaaccc gccagcatgg ttaataccgc
    18601 cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg
    18661 tgttagataa gcctggaata cattgggcgc tttttcaagc ccgcgaacga aacggctccg
    18721 ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt
    18781 tcagtggcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg
    18841 acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag
    18901 gaaattcgtc gtagtatcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa
    18961 tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag
    19021 atggcgatta atgagctgcg cctggaaacc ggcaacgaat cactgcaatt tattgccgta
    19081 cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat tgcctttatt
    19141 caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggccag cgaacaggca
    19201 ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag
    19261 cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat
    19321 catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg tacagacatt
    19381 aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt tactggcggc attaggttgc
    19441 ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg cccttctctg
    19501 ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa
    19561 aacgtacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa
    19621 cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt tgcacataat
    19681 ggtttgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattcgac
    19741 ctacaaaact atgcaaattc aatatattgc aggagtcgaa taggcctgac aggcgtagca
    19801 cgtcagacgg tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctc cccgcctgtt
    19861 acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg
    19921 gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt cattccttaa
    19981 tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct
    20041 ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga
    20101 tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgtaccg caggcgaaat
    20161 cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg
    20221 gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt
    20281 gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag
    20341 gcgcgcagct ttgcaagcat tgcagaccat cgctgatgaa caaaaacttt gttatggcct
    20401 gctgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg
    20461 cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc
    20521 tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca
    20581 gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa
    20641 ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta
    20701 taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc
    20761 gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc
    20821 cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag caatttatcg
    20881 cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc aaaggtagta
    20941 aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgtgt catgatgttg
    21001 aaatccatcg acatctgccc ttccgtcagt ttcgctttta ccacctggtc cgctgcaaag
    21061 gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc
    21121 tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga aaactcccca
    21181 ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa
    21241 gtgcaaattt cgcgcgtttc accggcagca tttcgccaca gattcaccga cattttacgc
    21301 atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa
    21361 ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac
    21421 aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag
    21481 tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag ttgcaggcat
    21541 tttacctttt g
//
LOCUS       DAEEBB010000065        21467 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEEBB010000065 DAEEBB010000000
VERSION     DAEEBB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.065"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(343..888)
                     /gene="hemG"
                     /locus_tag="KLF07_002304"
     CDS             complement(343..888)
                     /gene="hemG"
                     /locus_tag="KLF07_002304"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBD9832308.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(900..2351)
                     /gene="trkH"
                     /locus_tag="KLF07_002305"
     CDS             complement(900..2351)
                     /gene="trkH"
                     /locus_tag="KLF07_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBD9832309.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2390..3004)
                     /locus_tag="KLF07_002306"
     CDS             complement(2390..3004)
                     /locus_tag="KLF07_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBD9832310.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3004..4335)
                     /gene="pepQ"
                     /locus_tag="KLF07_002307"
     CDS             complement(3004..4335)
                     /gene="pepQ"
                     /locus_tag="KLF07_002307"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBD9832311.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4525..6714
                     /gene="fadB"
                     /locus_tag="KLF07_002308"
     CDS             4525..6714
                     /gene="fadB"
                     /locus_tag="KLF07_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBD9832312.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6724..7887
                     /gene="fadA"
                     /locus_tag="KLF07_002309"
     CDS             6724..7887
                     /gene="fadA"
                     /locus_tag="KLF07_002309"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBD9832313.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8077..8778)
                     /gene="fre"
                     /locus_tag="KLF07_002310"
     CDS             complement(8077..8778)
                     /gene="fre"
                     /locus_tag="KLF07_002310"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBD9832314.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8825..10318)
                     /gene="ubiD"
                     /locus_tag="KLF07_002311"
     CDS             complement(8825..10318)
                     /gene="ubiD"
                     /locus_tag="KLF07_002311"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBD9832315.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10485..10973
                     /gene="rfaH"
                     /locus_tag="KLF07_002312"
     CDS             10485..10973
                     /gene="rfaH"
                     /locus_tag="KLF07_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBD9832316.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10970..11752)
                     /gene="tatD"
                     /locus_tag="KLF07_002313"
     CDS             complement(10970..11752)
                     /gene="tatD"
                     /locus_tag="KLF07_002313"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBD9832317.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11794..12570)
                     /gene="tatC"
                     /locus_tag="KLF07_002314"
     CDS             complement(11794..12570)
                     /gene="tatC"
                     /locus_tag="KLF07_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBD9832318.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12573..13088)
                     /gene="tatB"
                     /locus_tag="KLF07_002315"
     CDS             complement(12573..13088)
                     /gene="tatB"
                     /locus_tag="KLF07_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBD9832319.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13092..13361)
                     /gene="tatA"
                     /locus_tag="KLF07_002316"
     CDS             complement(13092..13361)
                     /gene="tatA"
                     /locus_tag="KLF07_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBD9832320.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13440..15080)
                     /gene="ubiB"
                     /locus_tag="KLF07_002317"
     CDS             complement(13440..15080)
                     /gene="ubiB"
                     /locus_tag="KLF07_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBD9832321.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15077..15682)
                     /gene="ubiJ"
                     /locus_tag="KLF07_002318"
     CDS             complement(15077..15682)
                     /gene="ubiJ"
                     /locus_tag="KLF07_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBD9832322.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15696..16451)
                     /gene="ubiE"
                     /locus_tag="KLF07_002319"
     CDS             complement(15696..16451)
                     /gene="ubiE"
                     /locus_tag="KLF07_002319"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBD9832323.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16546..17973)
                     /gene="rmuC"
                     /locus_tag="KLF07_002320"
     CDS             complement(16546..17973)
                     /gene="rmuC"
                     /locus_tag="KLF07_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBD9832324.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18114..18875)
                     /gene="udp"
                     /locus_tag="KLF07_002321"
     CDS             complement(18114..18875)
                     /gene="udp"
                     /locus_tag="KLF07_002321"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBD9832325.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19137..19949
                     /locus_tag="KLF07_002322"
     CDS             19137..19949
                     /locus_tag="KLF07_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD9832326.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20343..21404
                     /gene="hcp"
                     /locus_tag="KLF07_002323"
                     /pseudo
     CDS             20343..21404
                     /gene="hcp"
                     /locus_tag="KLF07_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5266 a         5742 c         5381 g         5078 t
ORIGIN      
        1 gaattacgtg tccactcttg agacttggta ttcatttttc gtcttgcgac gttaagaatc
       61 cgtatcttcg agtgcccaca cagattgtct gataaattgt taaagagcag tgccgcttcg
      121 ctttttctca gcggcgcggg gtgtgcataa tacgctttcc cgctacagag tcaagcattt
      181 atttttgctt tctctgtcgg aattctcagg agaaccccgc tgacccggcg gcgtgtttgc
      241 cgttgttccg tgtcagtggt ggcgcattat agggagttat tccggcctga caagaggaaa
      301 tttaaaataa ttttctgacc gcgcattttt tattctttat gcttatttca gcgtcggttt
      361 gttggttaac tgggcgattt ctcgggcgaa attcgccacc tgctcccaat cggtatagac
      421 aacttctttg cgcgtatccg tttcaccgcc tgacatcttc ataattagct tgatcataaa
      481 acgatcgtac cagcgataac gtgggtaacg cagcgccccg gcaatgaccg cgcagcgatc
      541 gggacgccat tgcgagttca tcagaaactt ccgcgcgtag ctgttggtct gtggagtacg
      601 cttctccggt ttgcgcgcca ccaggtttac ggaataaaag gcgctcggca tcgaattcag
      661 tcgcgtcgca tgttttttga caaattcctg gaacgcagag tggtagtgac catagcgaat
      721 agaagcacca atgaccacac ggtcataatt ttcccattgt ggttcttcaa tgcggtgcac
      781 attggcgaca tccgtctgga tccccagttc tttcagttcc gaagccaggt aggaggcaat
      841 ctcgcgcgtt tgtccgtccc ttgttgagaa aagaattaat gttttcacgt attactccat
      901 cattcacgcc agaaagtcgg ggtaaagagc accagcaatg taaagacctc gagacgacca
      961 aacagcatgt tggcaatcag gatccattta gccaccgggt tcatactggt aaagttatca
     1021 gcaaccacgc caagccctgg ccccaggtta ttcaatgtcg caacaactga agcaaaggca
     1081 gaaaagtcat ccacgcccgt ggcgataatc gccagcatac tgacaataaa caccaatgca
     1141 taggcggaga aaaatcccca aacggcttcg aggatacgtt ccggcagtgc gcgattcccc
     1201 agcttaatgc tatagacggc gttcggatgc accagtcgtt tcagctcacg gttcccctgc
     1261 ttaaacagca gcaggatgcg gatcactttc aggccaccgc ccgttgaccc ggcgcaaccg
     1321 ccgataaaag ctgaacataa aagcagtacc ggcaaaaaga gcggccagcg ggcaatgctg
     1381 tcagtcgtaa acccggcggt tgtcgccatc gataccacct ggaaaaacgc ctggttaatt
     1441 gtcatcagcg ccgaactgta gacattatga aaccacagta cgagtgtaca aataaccacc
     1501 agcgtaaact gtacgccgat aaacatgcga aattccggat cgcgccaata aaccttcaga
     1561 ctacgcccac ttaacagtga aaagtgcaga ccgtagttac agccggagat cagcaggaag
     1621 atagcaatga tagtgttaat agtcgggctg tcgaaataac cgatactggc atcatgtgtc
     1681 gagaagccgc caatagcgat agtcgcaaag ctatggccga tggcatcaaa ggcatccatt
     1741 ccggcaaacc acaacgccag cgcacaggcg acggtcagca agacataaat caaccacagg
     1801 gttttcgccg tttccgcaat tcgcgggcgc attttgttat ctttcagcgg gccgggcatt
     1861 tctgcgcgat agagctgcat cccacccacg ccgaggatag gcagtatcgc aaccgctaac
     1921 acgatgatcc ccatcccgcc aaaccattgc agcatctggc gataaaagag gatggcatga
     1981 gggagcgaat ccagccccac cagcgtagtg gccccggtgg tggtcaggcc agagaaagat
     2041 tcaaaaaacg catcggtaat cgtgaggttc gggctttccg agaagataaa agggagcgca
     2101 ccgacactac ccagcacggt ccagaacagc accactatca gaaacccctc acgggatttc
     2161 agttcgccct tctctttacg gttcggccac cacagcatag aaccaatggc gagggcgaca
     2221 aaaaaggtct gggtaaaagc gcggcccgct ccatcccggt agatgagtgc taccagccca
     2281 gggataatca tggtccctga aaataagatg accagtagtc caacgattcg ggtaatggcg
     2341 cgaaaatgca tctctgccgc ttccttagtt ctgcaaaacg aagtggggat tattcttcaa
     2401 tcgctaacaa ttgcaatgaa ccacggctaa aatccgccag ctttgcggaa aattcagcca
     2461 ctttcgccgc cggaagcgcc acccgcagca gaacgaatgc ctgataatca ctgttgataa
     2521 ttttgccgtc acactgcccc agcaacgctt caatgccggt taactgatga tattcacatt
     2581 gcaaagtata ttcggttaat ggcgtcttgc gttgggtcgt tagctggcgc agcgcctgat
     2641 tcacgccgcc gccatacgct ttcactaacc caccggtgcc aagcaatatg ccgccgtagt
     2701 agcgcactac cacagaggta atttccccga cgccgctgcc cattagctgg gcgagcatcg
     2761 gtttacctgc cgttcctgcc ggctccccgt cgtcagagaa acccagctgt tgagaatcat
     2821 ccggcgcacc cgccacccac gccacgcaat ggtggcgggc atcggggtgt tctgcccgca
     2881 ccgattcaac aaacgctttc gccgcctcaa cgccatcggt atgcgccaac atcgttatga
     2941 agcggctttt tttgatctct tcaacgaccg tgaccggtgc cgcaggaatt aaccagcttt
     3001 ccatcacgcc agtttcagat cccgggtcat gttttccacg ttgttttcgt ggatcaccac
     3061 gttgtcttcg atacgaatgc cgccgaacgg tttcagtgct tcaattttct gccagttgaa
     3121 gtgcttgctg aactgccctt cacgccacgg tgccagtagc gattcaatga agtagatacc
     3181 cggttcgatg gttaacacca tacccggctg gagaatacgg gtgcagcgca ggtacggata
     3241 ttttgccggt gccgcgaggt gtgtaccgct atcatcctgc ataaaaccgg ctacgtcatg
     3301 cacctgcagg cccagcggat gaccgatacc atgcggcata aacggtccgg tgagatcgtt
     3361 ttcgaccatc gcttcttcac tcatatcggt gatgatttga tgtttacgca gcagtttggc
     3421 gatgcgctga tggaactgga tgtggtaatc cacatagcta acgcctgctt tcatggtggc
     3481 gatcagcgcc agttgttcat catttacgtc tttcaccagc tgtgcgtagt cgttgtcgct
     3541 ttttgccgac caggtacgag tcaggtcagc cgcatagccg ttatattcgg ccccggcatc
     3601 cagcaggaag ctgcgcatct cttccggtgc ctgatgatcc agtttggtgt aatgcagcac
     3661 cgaagcgtgt tcgttaagcg ccacaatgtt gctgtaaggt acgtcggtat cacgatgacc
     3721 ggtcgcggtc agataggcga tgttgatatc gaactcgctc atgccagaac ggaacgcttc
     3781 ttctgccgca cgatgaccgt tgaccgccat tttctgcgct tcacgcatac aggccagttc
     3841 gtactcggtt ttgaaggagc ggtagtaatg caggtagtcg atcacccctt tcgggttgat
     3901 gttgctggcc tcaataccca gttgcagcgc acgttccggc accggaccga tataaccgat
     3961 attgccgcgc gcagcgggca gcaggctacc aatgccatcg gctttcggca gtgcgatcac
     4021 ttccacatct tcagtccaga aggaggtcgg cagcggttcg acgttgtgcc agtaatcaac
     4081 cggcagatag aaccacagtt tcggcttgtt cacgccatcc accagcaacc agcagtttgg
     4141 cacctgagtt accggcaccc acgctttgaa ttgcgggttc actttaaacg gatagggatg
     4201 atcgtcgaga aaaacgttga acagctcgcc ggagtgaata agtaatgcat ccagcttgaa
     4261 gcgcgccagc gcatcgcgag tccgttcttg taaggtagct atatgatttt tatagagcga
     4321 ggccagtgat tccatttttt acccttctgt ttttttgacc ttaagtctcc gcatcttagc
     4381 acatcgttca tccagagcgt gatttctgcc gagcgtgatc agatcggcat ttctttaatc
     4441 ttttatttgc atatttttaa cacaaaatac acacttcgat gcatctggta cgaccagatc
     4501 accttgcgga ttcaggagac tgacatgctt tacaaaggcg acaccctgta ccttgactgg
     4561 ctggaagatg gcattgccga actggtattt gatgccccag gttcagttaa taaactcgac
     4621 actgcgaccg tcgccagcct cggcgaggcc atcggcgtgc tggaacagca atcagatcta
     4681 aaagggctgc tgctgcgttc gaacaaagca gcctttatcg tcggtgctga tatcaccgaa
     4741 tttttgtccc tgttcctcgt tcctgaagaa cagttaagtc agtggctgca ctttgccaat
     4801 agcgtgttta atcgcctgga agatctgccg gtgccgacca ttgctgccgt caacggctat
     4861 gcgctgggtg gtggctgcga atgcgtgctg gcgaccgatt atcgtctggc gacgccggat
     4921 ctgcgcatcg gtctgccgga aaccaaactg ggcatcatgc cgggctttgg cggttctgta
     4981 cgtatgccac gtatgctggg cgctgacagt gcgctggaaa tcattgccgc cggtaaagat
     5041 gtcggcgcgg atcaggcgct gaaaatcggt ctggtggatg gggtagtcaa agcagaaaaa
     5101 ctggttgaag gcgcaaaggc ggttttacgc caggccatta acggcgacct cgactggaaa
     5161 gcgaaacgcc agccgaagct ggaaccgcta aaactgagca agattgaagc caccatgagc
     5221 ttcaccatcg ctaaaggaat ggtcgcgcaa acggcgggga aacattatcc ggcccccatc
     5281 accgcagtaa aaaccattga agctgcggcc cgttttggtc gtgaagaagc cttaaatctg
     5341 gaaaacaaaa gttttgtccc gctggcgcat accaacgaag ctcgcgcact ggtcggcatt
     5401 ttccttaacg atcaatatgt aaaaggcaaa gcgaagaaac tcaccaaaga cgttgaaacc
     5461 ccgaaacagg ctgctgtgct gggcgcaggc atcatgggtg gcggtatcgc ttaccagtcc
     5521 gcgtggaaag gcgtgccggt tgtcatgaaa gatatcaacg acaagtcgtt aaccctcggc
     5581 atgaccgaag ccgcgaaact gctgaacaag cagcttgagc gcggcaagat cgatggtctg
     5641 aaactggctg gcgtgatctc cacgatccac ccaacgctcg actacgccgg atttgaccgc
     5701 gtggatattg tggtagaagc ggttgttgaa aacccgaaag tgaaaaaagc cgtactggca
     5761 gaaaccgaac aaaaagtacg ccaggatacc gtgctggcgt ctaacacttc aaccattcct
     5821 atcagcgaac tggccaacgc gctggaacgc ccggaaaact tctgcgggat gcacttcttt
     5881 aacccggtcc accgaatgcc gttggtagaa attattcgcg gcgagaaaag ctccgacgaa
     5941 accatcgcga aagttgtcgc ctgggcgagc aagatgggca agacgccgat tgtggttaac
     6001 gactgccccg gcttctttgt taaccgcgtg ctgttcccgt atttcgccgg tttcagccag
     6061 ctgctgcgcg acggcgcgga tttccgcaag atcgacaaag tgatggaaaa acagtttggc
     6121 tggccgatgg gcccggcata tctgctggac gttgtgggca ttgataccgc gcatcacgct
     6181 caggctgtca tggcagcagg cttcccgcag cggatgcaaa aagattaccg cgatgccatc
     6241 gacgcgctgt ttgatgccaa ccgctttggt cagaagaacg gcctcggttt ctggcgttat
     6301 aaagaagaca gcaaaggtaa gccgaagaaa gaagaagacg ccgccgttga agacctgctg
     6361 gcagaagtga gccagccgaa gcgcgatttc agcgaagaag agattatcgc ccgcatgatg
     6421 atcccgatgg tcaacgaagt ggtgcgctgt ctggaagagg gcattatcgc cactccggcg
     6481 gaagcggata tggcgctggt ctacggcctg ggcttccctc cgttccacgg cggcgcgttc
     6541 cgctggctgg acaccctcgg tagcgcaaaa tacctcgata tggcacagca atatcagcac
     6601 ctcggcccgc tgtatgaagt gccggaaggt ctgcgtaata aagcgcgtca taacgaaccg
     6661 tactatcctc cggttgagcc agcccgtccg gttggcgacc tgaaaacggc ttaaggagtc
     6721 acaatggaac aggttgtcat tgtcgatgca attcgcaccc cgatgggccg ttcgaagggc
     6781 ggtgcttttc gtaacgtgcg tgcagaagat ctctccgctc atttaatgcg tagcctgctg
     6841 gcgcgtaacc cggcgctgga agcggcggcc ctcgacgata tttactgggg ttgtgtgcag
     6901 cagacgctgg agcagggttt taatatcgcc cgtaacgcgg cgctgctggc agaagtgccg
     6961 cactctgtcc cggcggttac cgtcaatcgc ttgtgtggtt catccatgca ggcactgcat
     7021 gacgcagcac gaatgatcat gactggcgat gcgcaggcag gtctggttgg cggcgtggag
     7081 catatgggcc atgtgccgat gagtcacggc gtcgattttc accccggcct gagccgcaat
     7141 gtcgccaaag cggcgggcat gatgggctta acggcagaaa tgctggcgcg tatgcacggt
     7201 atcagccgtg aaatgcagga tgcctttgcc gcgcggtcac acgcccgcgc ctgggccgcc
     7261 acgcagtcgg gcgcatttaa aaatgaaatc atcccgaccg gtggtcacga tgccgacggc
     7321 gtcctgaagc agtttaatta cgacgaagtg attcgcccgg aaaccaccgt ggaagccctc
     7381 gccacgctgc gtccggcgtt tgatccagta agcggtacgg taacggcggg tacatcttct
     7441 gcactttctg atggtgccgc ggccatgctg gtgatgagtg aaagccgcgc ccgtgaatta
     7501 ggtcttaagc cgcgcgcccg tgtgcgttcg atggcggtcg ttggttgtga tccgtcgatt
     7561 atgggttacg gcccggttcc tgcctcgaag ctggcgctga aaaaagcggg gctttctgcc
     7621 agcgatatcg gcgtgtttga gatgaacgaa gcctttgccg cgcagatcct gccgtgcatt
     7681 aaagatctcg ggctaatgga gcagattgac gagaagatca acctcaatgg tggtgcgatc
     7741 gcgctgggtc atccgctggg ttgttccggt gcgcgtatca gcaccacgtt gctgaatctg
     7801 atggaacaca aagacgttca gtttggtctg gcgacgatgt gtatcggtct gggtcagggt
     7861 attgcgacgg tgtttgagcg ggtttaacca cttgccggat gcggcgtgaa cgccttatcc
     7921 ggtctaccga tcctgcacca atgtaggcct gataagacgc gccagcgtcg catcaggcat
     7981 cgtgcaccga ttgccggatg cgacatagat ctaaatgcca ctgaataaag ttttagttgc
     8041 cgttcttccc gcctgtcagg ggcgggtttt tatatctcag ataaatgcaa acgcatcgcc
     8101 aaacagacga tcttcccgcg cattacgctc actgcaaaac aggtcacgag caattttcgc
     8161 catctcaaaa cgtccggcaa tatagatatc atgctctgcc agcgtaccat gatcctgcaa
     8221 gaccgccgtt aacacggtgc cagtacgccc acgccagccc gcttccggtt gttcaaccac
     8281 cggcaccact tgcagaccag gatgcttcag cgaaagcgcc tcaagctcgc agagatcata
     8341 cagatgttgc tcttcacgcc caccccagta aatggtgata tcgcgattgg gattacgcgc
     8401 caacgccgtc aataaaatcg agcgcgcata ggagaacccg gtgccgcccg caatcaaaat
     8461 catcggacgc tcttcatcat cgcgcagcca cgcttctccg tggggaatat cgaccacgat
     8521 ttgatgatct ttgaggatgc ggtccatgac tgctttcgcg taaaggttga tttcagaagc
     8581 gccaatatgc agctcgataa accctttttc atccggcgtc gaagccattg aaaacggacg
     8641 tttgtcgcgc tcatccatca ctaccatcaa atactgacca gcacgaaaag aaaaggccgc
     8701 gtctggtacg attcggacac ggtatacagt atccgtgata gcttctaccg aggtcacttt
     8761 acagcttaag gttgtcatgc gctttctctg tcggatcaaa taaatagggc aaaactaacg
     8821 cgcatcaggc gcttttaccg ttgttaaaaa tagccagttc atcccagatg gcgtcaatat
     8881 gcgcgacgac atcaggatct tttttgatgg gacgtcccca ttcacgctgg gtttcccccg
     8941 gccatttatt cgtggcatcc agccccattt ttgaacccag cccggagaca ggcgaggcaa
     9001 aatccagata atcaataggc gtattttcta ccagaacagt atcccgcgcc gggtccatac
     9061 gggtggtaat cgcccaaatc acatcgttcc agtcgcgtgc gttgacgtca tcatcgcaaa
     9121 cgatcacaaa tttagtgtac ataaactggc gtaagaacga ccagacgccc atcatgacgc
     9181 gcttcgcgtg tccggcgtac tgttttttga ttgtcactac cgccagacga taagaacagc
     9241 cttccggtgg caggtaaaaa tcgacaattt ccgggaactg tttttgcaga atcggcacaa
     9301 acacttcgtt cagtgcgaca cccagcaccg cgggttcatc cggcggacga ccagtatagg
     9361 tggagtggta aatcgcatct tcacgctggg taatatgcgt cacggtaaat accgggaaac
     9421 tatcgacttc gttatagtaa ccggtgtggt cgccatacgg cccttccggc gcagtttcgc
     9481 cttgttcgat atacccttcc agcacaatct ccgcactggc gggcacttca aggtcattgg
     9541 agatacactt caccacttcg gtcttggtac cgcgcagcaa tccggcaaac gcatactctg
     9601 aaagtgtatc cggaacgggg gtgactgcac cgagaatcgt ggcgggatcg gcacccagcg
     9661 ccacagaaac cgggaaacgt tcgcccggat gcgccgcaca ccactcctga taatccagcg
     9721 cgccgccgcg atgcgacagc cagcgcataa tcagtttgtt tttaccaatc agctgctggc
     9781 gataaatgcc cagattctgc cgctctttat gcgggccgcg cgttacggtc agcccccagg
     9841 taatcagcgg cgcggcatct tccggccagc aggtcataat gggaatgcga ttgagatcga
     9901 cgtcatcgcc agagacgatt ttttgttggc agggcgcacc acgcagccgc tttgtcggca
     9961 tgttcaatac ttgcttaaac tgcggcagtt tatcaaacag gtcgcggaaa ccttttggcg
    10021 gctcgggctc tttcagaaac gccaataatt taccaacttc acgcagcgcc gaaacatctt
    10081 cctgccccat gcccatcgcc acgcgctttg gcgtaccgaa caggttgcac agcaccggca
    10141 ttgagtagcc tttagggttt tcgaacaaca gcgcaggccc accggcacgc aaagtgcggt
    10201 cagcaatttc agtgatttcc agatgcggat ccaccgggag cgtgatacgt tttagctcac
    10261 cctgctgttc aagcagcgtc aagaagtcgc gtaaatcgtt atatttcatg gcgtccattg
    10321 tagcctctta atctacgccc attatacggc gttcatcttt gcgatgctgt aaatttgtta
    10381 aattagcgtg aactctgacg gtataacgca aaccggggaa tataattaac ttagcgtaaa
    10441 gcttttgcta tccttgcgcc ccgattaaac ggataagagt cattatgcaa tcttggtatt
    10501 tactgtactg caagcgcggg caacttcaac gtgcccagga acaccttgag agacaggctg
    10561 tgaattgcct ggcaccgatg atcaccctgg aaaaaatcgt gcgtggaaaa cgtactgcag
    10621 tcagtgagcc attgtttccc aactacctgt ttgtcgaatt tgatccagaa gtgattcata
    10681 ccacgactat caacgcgacc cgcggtgtca gccacttcgt gcgctttggc gcgtcgctag
    10741 cgatagtccc atcggcggtg attcatcagc tatcggtata taaaccgaaa gacattgtcg
    10801 atccggcaac cccttatccg ggagataagg tgattattac cgaaggcgcg ttcgaaggct
    10861 ttcaggccat tttcaccgaa cccgatggtg aggctcgctc catgctattg cttaatctta
    10921 ttaataaaga gattaagcac agtgtgaaga acaccgagtt ccgcaaactc taaaacgcaa
    10981 tcccaaaccg tgttttgaca ttagcatccg tggtggcagc cagccatgcg gcatcttctc
    11041 cacgccagtg cgcaatacgt tgcaaaatat ggggcagatg ggctggctcg ttgcgccggg
    11101 atgatggctt tggcgtgaga tcgcgaggga gcagatacgg cgcatcagtt tcgatcagta
    11161 atttttccgc cggaatcaac ggcaaaagtt cccgcagctc cagtccgcgt cgttcatcgt
    11221 aaacccaacc ggtaatgccg atataaattc catgcgccac gcacgcctgc atctcttcgc
    11281 gtgtgccggt aaagcaatga agaaccgcac caggcagttt atccagccac ggctccagca
    11341 atgtcataaa ccgctcgtgg gcatcgcgac agtgcataaa taccggcatg tttaaatctg
    11401 cggcaatgcg tagctgggca acaaaagcgc gttcctgctc ttccggcgtc gaaaagttgc
    11461 ggttaaagtc gagaccacat tcaccaatcg ccaccacttc tggctgcgcg gccagctcaa
    11521 taatcgcttc ttcagtcgca gcttgccact ggctgctgtc gtgaggatgt acgcccgccg
    11581 ttgaccaaca ggacgaatac tgacgcgcca gcttttgcgc ctgctggctt tcacgcaggt
    11641 tagtgccggt gatgagtagc ccattaactc ccgcgtcaaa agcgcgcgct acaacatcat
    11701 cacggtcttt cgcaaattgc gaactggtca aattaacgcc gatatcaaac atcctgtact
    11761 ccatatgaca accgccctga cgggcggttg aatttattct tcagtttttt cgctttctgc
    11821 ttcagcgtcg ttttcctctt cccgatttcg ccctttacca acgtaaaagc gtgagaagaa
    11881 gacaccgatt tcaaacagac agtacatcgg gatcgccaat agcgtttgcg agaagacatc
    11941 cggcggcgtc agcaacatcc cgacaacgaa tgcaccaacc agcacatacg ggcgtttttt
    12001 gcgtaagtct tctggcgagg taatccccat ccagcacagc agcacaattg ccaccggcac
    12061 ttcaaaggag acaccaaacg ccataaacag cgccataacg aagcttaaat agctggcgat
    12121 gtcggtggat acctgcaccc cttccggcgc ggtattggca aggaagccaa atgccagcgg
    12181 aaagaccaca aagtaggcga atgccatgcc gatataaaac agcagagagc tggaaaccag
    12241 cagcggcacc accaggcgac gttcatgctt atacagcgct ggggcgataa atgcccacac
    12301 ctgatagaga atcaccggcg ctgacagaat cagcgacacc ataaaggtca gcttgatcgg
    12361 cgtaaagaac ggcgaggcca cgtcggtggc gatcatcgtt gaaccttgcg gcaactgctt
    12421 gatcaatggc gcggatacca ggtgatagat gtcattggcg aaatagacca gacacaggaa
    12481 tatcacgatc accgcgataa tgcagttcag cagacgctta cgcagctcaa ttagatgcgt
    12541 gataagcggt tgagtatctt ctacagacat gtttacggtt tatcactcga cgaaggggaa
    12601 ggtgcagcgg ttttcggttc agcgtccgca gcaggtttta ccaccggctc tggcgtggtt
    12661 tctggcttct gttccggcga actggcctgc gtttgtgcag cggcaggcgt tacgccctca
    12721 tgcgcagctt cattatcttt caccaccggg ttatggatgg tgtgcgcttc atcgctcgcc
    12781 ttttcaggat cgtttgcaac gtaggaacgc ttcatcgact ccgcggcctg gcgtagttca
    12841 tccatcgacg ctttcagttc gggcgtcagg ttagtgaggc tcgccttttc aacctttttc
    12901 agactgtcct gaaactcctg gagttttaac tcctgggtca gttcgttctg caccgttgtc
    12961 gccagtgaac gcaacgcgcg aatccagccc gctaccgttt ttaccgccac aggcagtcgt
    13021 tgcggcccca gaacgacgag gccgatgatg aacaccaata gcagttcgct aaaaccgata
    13081 tcaaacacgg attacacctg ctctttatcg tggcgcttcg cgtcttctgt tttagcctgt
    13141 tcctgattcg tatccgcctg cttatcggcg atagttttcg cagtaaaatc agcatcctga
    13201 ctggttttat cctgctttgg ttcatcatcg ctcattgctt ttttaaagcc tttgatcgac
    13261 gcaccaagat cggaaccgat ggagccgagc tttttggtgc caaaaagcag tacaacgatg
    13321 acggcaataa tcaataactg ccaaatactg ataccaccca tacatgttcc tctgtggtag
    13381 atgatgatta aacaaggccg cattatacgt tacacgaccc gccttgagcg atgaaaaaat
    13441 cagcgtgttt tgcgccaacc gacaaaccag gcgatcagac cacctgccat taaccagccg
    13501 ggcatcagcc cccattcagg tcggctgacc aacaagaatg tgccacttaa tactaacgta
    13561 gcgccaattc cgagaaaata acgcgattgt ccctgacgta catgatttga ctgaagctcg
    13621 cgggcaatct tatcaacact gtgctgtaaa tacttgccct ggcgcaaact gtcgtaaacc
    13681 aattcaggca gttctggcat tttttcgacc cagaacggcg ctttttcttt aaatgctctc
    13741 accagggcag gaataccgac ctgatcttta atccacgact ccaggaaagg cttcgctgtt
    13801 ttccataaat cgagctgcgg ataaagctgg cgtcctaccc cttcgacata gagcagggtt
    13861 ttctggagta acaccagttg cggctgcact tccatattga agcgacgcgc cgtattaaac
    13921 agatttaaca gtacatgtcc aaacgaaatt tcggccagcg gtttctcaaa gataggttca
    13981 cagaccgtac gaatggcaaa ttcgaactct tcaacgttgg tatctggtgg cacccagcca
    14041 gaatcgacgt gtagctctgc cactttgcga tagtcgcgat taaagaaggc gataaagttt
    14101 tctgccagat agcgtttatc ttctttgttt agcgagccaa caatcccgca atcaatgccg
    14161 atatatttcg ggttttccgg gtgttcatag cttacgaaaa tgttgccagg gtgcatatcg
    14221 gcatggaaaa agctgtcgcg aaagacctga gtgaagaaca cctgcacgcc acgttccgcc
    14281 agcaatttca tgttggtgcc atttttctcc agcgtcgcaa catcagacac cggaatgccg
    14341 taaatgcgct ccatcaccat catcccttca ctacaatagt cagggtaaac ttccgggatg
    14401 tagagcatcg ggctgtcttc aaaatttcgc cgaagctgaa tggcgttggc agattcccgc
    14461 agcaaattca gttcatcaat caaggtcttt tcgtactcgc gcaccacttc ggttgggcgc
    14521 agacggcgac catccggcag caaacgcggc acccagcgag ccagacggta gataagtttc
    14581 agatccgctt taataaccgg caaaatatcc gggcggatga ctttaatcac cacctcttta
    14641 ccattcgatt tcaatcgcgc ggtatgaacc tgggcgatag aagcagaagc cagtggcttg
    14701 atttcaaaat cgtcaaacca cgcttctacc ggcaagccgc ccattgcagc ttcaatctgc
    14761 tgcttcgcca gcttgccatc aaacggagca actttgtcct gcaataacgc cagctgatcg
    14821 gcaatatgcg gtggaaaaag atcgcggcgg gttgataaca tttgcccgaa cttgatccaa
    14881 accggcccca gttcttgcag ggccagtcgt agtcgctcac ctaaaagttt gtctttatgc
    14941 cgatttggca tccagaataa tgagtatcgc catagccgta gcggcagggt gatacgcatt
    15001 ttggggatca gttcatcaag tccgtagctt aaaaaagtgc gaatgatgaa atataggcgc
    15061 cgtacttcac ctggcgtcat ttagcctcca gtttttccag ccgtttggtc agggcatcaa
    15121 cagcacgctc gacggcagcc gtctcttccg caaaccaggc cacttcaagc ggaccgggtg
    15181 ccatacgcca ctcttcagta atggcttccg ccacataacg ttgctggcgc ttaatgccgt
    15241 gatgcaggaa ctttgcgcct ccgcgcatgg ctttgctgat tccttcagcg gcgatatcac
    15301 cggtataagg ggccagcagt tccgcagggt cgaactctgc cagatctgcc agcgcaacga
    15361 agttttgcac cacctgaata tcgccctgca cttccagctc accactgcga atcagtgcgg
    15421 taagctgctg gcgatcgcga agtttcggca acacactggc gtaggcgata acggtgcagt
    15481 cagcatcgcc tgcccattcg cccagtacat caacctggcg ttcgctgaac accagaatca
    15541 atgacgtcga aaagcctttt acctccacgc gcaatacttt acccagcaga cgcgagcggg
    15601 ctgttttcag cgcgggtgag cgatacagga aggtgttgag cagactttca attcctgccg
    15661 tcactaaagg tttaaaaggc atttccggtc tcctgtcaga acttataacc acgatgcagc
    15721 gccacaaccc ctgccgtcag attgtagtag tcgacacttt cgaatccggc atcctgcatc
    15781 atggctttca gggtatcctg atcgggatgc atacggatgg attctgccag ataacggtag
    15841 ctgtcggcgt cgttcgcgac cagtgagcca atacgcggca gcacatggaa ggagtatgca
    15901 tcataggctt tgctcagcgg ctcgataatt ggcttcgaga actcaagcac cagcaggcgg
    15961 ccgccgggtt tcagcacgcg atacattgaa cgcagtgctt tatctttgtc ggtgacgtta
    16021 cgcagaccaa acgaaatggt gatgcaatca aaggtgttat ccgggaacgg cagcgcctca
    16081 gcgttcgcct gaacatactc aacgttgcca atcacaccga tattacgcag cttctcgcgt
    16141 cccattttga gcatggattc attgatatca gcaaggacca ctttgccagt ttctccgacc
    16201 aggcgggaga atttcgctgt caggtcgccg gtgccaccag ccagatccag cacggtctgc
    16261 ccacggcgta cgccgctgca atcaatcgtg aatcgcttcc acaaacgatg aataccaaat
    16321 gacatcaaat cattcatgac atcgtatttt gatgccacgg aatggaaaac gtgggcgacc
    16381 atatccgctt tttgttcctt cgcgacggtc tgaaaaccaa agtgcgtcgt ttcttgtgac
    16441 ttatccacca tctcaatgcc tgctcatcaa aaaattgttc cagaagtgta acagattggg
    16501 cgtcgatgcc ctagatttct acccggctta actaccccta atgggctagc gcgactgctg
    16561 attatattca tcatcgcgtt gataagcttc atcattcggc tgctccggaa ccgaccgaag
    16621 tcgatactct tcattctggc tcaccgcctg ttcagccaaa tccggattaa tctcgcgttt
    16681 aatttctact cctaaaccgc gaaacgcttc tgcctgcgcc agcacatttc cgcgacctga
    16741 agagagtttt ttcattgcct ggcggtagtt atcctgcgct ttgtcgagac tttgaccaat
    16801 cgcggacatg tcatcgatga acaaacgcat cttgtcgtac agcttgctgg cacgatcggc
    16861 gatttgctgg gcgttgcggc tttgatgctc ataacgccac aggttggcaa tagtacgcaa
    16921 cgccaccagc agcgtagtcg ggctaaccag catgatattg tttttcaacg cttcggtgat
    16981 cagctccggc tggcggtcaa gcgccagtaa aaaagcgggt tcaacgggaa taaacatcag
    17041 cacgtaatcc agagttcgca gccccggcag ctgttgataa tctttgcgtc ccagcaaacg
    17101 gatatggtta cgcaccgacg cgatatgttc ctgtagcgcg ctttcgcggg tgtagtcgtc
    17161 ttcggcgtta aaatagcgtt cataggcgac cagcgtcatt ttggcgtcga tcaccacatc
    17221 ttttccctgc ggcagccgca cgatgacatc cggctgcatc cgcgagcggg cgtcattttc
    17281 gatgctgacc tgggtttcat attcataccc ttcacgcaga ccggaagcct ccagcacccg
    17341 cgtcaatact acctcgcccc agttgccctg ggttttattg tcgcctttca gcgcgcgcgt
    17401 caggttgatc gcttcctggg ccatttgcgc gttgagttgc tggagattgc gaatttcgtg
    17461 ggtcagggta tggcgttctt gtgcttcttt accgaagctg tcctgaacct gacggcggaa
    17521 accgtccagt tgttcacgta gcggcgacaa caggctgttc agactctgac ggttttgctc
    17581 atcaacccgg cgattgctgt gctcaaaaat acggttggcg aggttttcaa actgctcact
    17641 gaggcgctgc tcgctgttaa tcatctggcg aattttatcg tcagcatgtt gctgtgcggc
    17701 ttccatccgc gtggttactt cacgcagatc ggcctccaga gaggtgttaa tactttgcag
    17761 gctgcgcact tcgttattga gtaactcgca ctctgcacgc cagtgctcgc tttgggtaat
    17821 ttgttgtttt gccgcgctta actccgcgac catctcttca cgttcagcta attgctcggc
    17881 tttttgctgc gcatgttgat aactggcaaa cagccagcca attgccacac ccaccaacgc
    17941 aataactgcg taaaccatga ttgagaaatc cacaacgcct cctgacgcaa caacttaccc
    18001 gcacaaaata gaattgtgct gtacaaacgt ccagttggaa aggcatttcc tgcgaggcgc
    18061 tacgcaaaaa tacaaaaggc cgaacgcgtc ggccttcaga caggagaaga gaattacagc
    18121 agacgacgcg ccgcttccac cacgattttc accgcttggc tttcagtttg tttcatcgtc
    18181 tcagcattcg ggatctcttg ctgggtgcgg ttaacgataa cacccgctac cataccggca
    18241 cgcaggccct gacttgcaca catggtcagc agggttgcag attccatttc atagttcatt
    18301 atgcccatcg cctgccactc ttccatagaa cctttaaagt gacgaactac gcgaccagag
    18361 taagtatcgt aacgttcctg acctgggtag aaggtatcag aagaagctgt cacgccaacg
    18421 tgagttgtcg cgccaatgga tttcgcagct tcaaccagcg cagtcgtaca ttcgaaatca
    18481 gcgacagccg ggaattccag cggtgcgaag tgcaggctcg cgccatccag acggacagac
    18541 gccgtggtaa ccaggacatc acccacatta atatgcggct gaatagcgcc cgttgtaccg
    18601 atacgcagga aggtgcgaat gcccagctgt gccagctctt caacagcaat agaggtagac
    18661 gggccgccga taccggtaga gcagacgata acaggtttac catccagctc tgcacgccag
    18721 gtagtgaatt cgcggtgaga tgccagctta accggcttat ccatcagcgc ggcgatcttt
    18781 tccacacgat ccgggtcgcc agggacgatg gcaagcgtag ccccttgtaa atcgttttta
    18841 gtgaggccga gatgaaaaac atcagacttg gacatataca actcctctgt gaatcggttt
    18901 agtcagaaga agcaaaaaga cactttaccg aagggtttaa cattttttcg tgatactcat
    18961 caccatgacg caaatgcgtt gcataaattt acccaccaaa agtgattcaa atcacataaa
    19021 tcactcttca ttctgcagga ctgcataaaa gatgagccga ttcaggcaat gtgatttgtt
    19081 gcatgctgac tatagttaac attgcgcaac atttttataa gggtacggag aataccatgg
    19141 caacaacaca acaatctgga tttgcacctg ctgcatcgcc tctcgcttcg accatcgttc
    19201 agaccctgga cgacgcgatt gtggcgggct tcacctctat cccttcacaa ggggataaca
    19261 tgcctgctta ccatgccaga ccaaagcaaa gcgatggccc actgccagtg gtcattgtag
    19321 tgcaggaaat ttttggcgtg catgaacata tccgcgacat ttgtcgccgt ctggcgctgg
    19381 aggggtatct ggctatcgca cctgaacttt acttccgcga aggcgatccg aatgattttg
    19441 ccgatatccc cacgttgctt agcggtctgg tagcaaaagt gcctgactcg caggtgctgg
    19501 ccgatctcga tcatgccgcc agttgggctt cccgcaacgg cggcgatgtt catcgtttaa
    19561 tgatcaccgg attctgctgg ggtggacgta tcacctggct gtatgccgcg cataatccac
    19621 agctaaaagc tgcagtggcg tggtacggca aactgacggg cgacaagtcg ctgaattcac
    19681 cgaaacaacc tgttgatatc gcaaccgatc ttaacgcgcc gattctcggc ttatatggcg
    19741 gtcaggataa cagcattccg caggagagcg ttgaaaccat gcgccaggcg ctgcgggctg
    19801 ctaatgcgaa agcagagatt atcgtgtacc cggatgccgg gcatgcattc aacgctgatt
    19861 atcgcccgag ctatcatgcc gaatctgcga aagatggctg gcagcgtatg ctggaatggt
    19921 ttaagcagta tggagagaag cggagttagt ttcctcttag tctatatttg agatggtgag
    19981 taaacgagtg cgttttgctg ccatctctgt cccccaaaaa ataatcagta tgaattgatt
    20041 ggagattacg actccgctat ttgaccttaa gaaagtcggc aagtcatagt cataaatgag
    20101 tctcttcaat ggaagacacc ttagagattt accatcaact cccattcaca aacaaacatt
    20161 aattattatc aacacattca cattaattat aaatcatctc agaccttcct gataaaaaga
    20221 tattaaagtg aaacattttg tttttcacat tttagttcat cacttttagg cgtaaataaa
    20281 ggtaaaaaaa acaacaatac tatttctctt tcacaaaaga attaacaaaa ggtttttcaa
    20341 ggatgagtga tattatttac ctgaccataa ccggagaaca acaaggatgt atttcctctc
    20401 gttgtggaac gagcgcatca ataggtaatc gctggcagat tggccatgat gatgaaatct
    20461 tcgcgttctc gctctctaat agtattacga atacaggcaa agggtcataa ttgcatggcc
    20521 ttagtttctg taaattgatt gacaaaagct ccccgttact tattaacgct attaacaata
    20581 acgagcagtt gttcatggag tttgacttct atcggatcaa taggtttggt cgatgggaga
    20641 aatattacaa cattcaactc cgcggcgcgc tcttatcagc aatcaatcac ctgtttactg
    20701 agaacaatct ggatacagaa gccatcactg tcagttatta atatatactc tccaggcatc
    20761 tgattgctaa tactgaattt agttattggg catttcccga taactacaac cgtctgttta
    20821 taccacggcc aaaaactaaa gctggtaatg gtttcaaaac acttaatagt aaagcggttg
    20881 gcagactgct tgctgctgtc ggcatataca atgggaatat cgaaggattc agggaaacag
    20941 cagacaagtt ggggggagat gcacctgctg gttatgatca atttatggat aacaaagggt
    21001 tactcatcgc tggtgcttca atagttgctg gagcagttct ggggagaatg agactccctg
    21061 atttggaatc acttgagcat ttcggagcca gaggagcagt atcaggaaga ccatttaatc
    21121 ccgagcttgc cggtggtcca atagaaaacc tgacgacaga tggcgtaaca atcaacaggg
    21181 aaggtatcgc tattgttgaa aaacatatag ccagatttgg tcatgatcct gtaaatgaag
    21241 tgatgatcaa tagactgaaa gacattgaga aaggaaaaat ccccccagaa caagtagatc
    21301 ttaacttcta tactcatgag tgtcgggaat atcagcgtta ctgtaatctt ggttgggaaa
    21361 caggacaacc tgatggtgac gcaggctatg cactctggaa tcatactgga tttgccccta
    21421 tatttccaga catctgttat cacttaaccc attacaagcc cgctgcc
//
LOCUS       DAEEBB010000066        21393 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEEBB010000066 DAEEBB010000000
VERSION     DAEEBB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.066"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            21..2393
                     /locus_tag="KLF07_002324"
     CDS             21..2393
                     /locus_tag="KLF07_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD9832327.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            2450..5233
                     /locus_tag="KLF07_002325"
     CDS             2450..5233
                     /locus_tag="KLF07_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD9832328.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            5595..6995
                     /locus_tag="KLF07_002326"
     CDS             5595..6995
                     /locus_tag="KLF07_002326"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD9832329.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            7151..8686
                     /gene="gadC"
                     /locus_tag="KLF07_002327"
     CDS             7151..8686
                     /gene="gadC"
                     /locus_tag="KLF07_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBD9832330.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            8817..10136
                     /locus_tag="KLF07_002328"
     CDS             8817..10136
                     /locus_tag="KLF07_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBD9832331.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            10508..11890
                     /gene="dosC"
                     /locus_tag="KLF07_002329"
     CDS             10508..11890
                     /gene="dosC"
                     /locus_tag="KLF07_002329"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBD9832332.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            11915..14314
                     /gene="dosP"
                     /locus_tag="KLF07_002330"
                     /gene_synonym="pdeO"
     CDS             11915..14314
                     /gene="dosP"
                     /locus_tag="KLF07_002330"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBD9832333.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            14573..15154
                     /gene="ddpX"
                     /locus_tag="KLF07_002331"
     CDS             14573..15154
                     /gene="ddpX"
                     /locus_tag="KLF07_002331"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBD9832334.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            15168..16718
                     /locus_tag="KLF07_002332"
     CDS             15168..16718
                     /locus_tag="KLF07_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD9832335.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            16720..17742
                     /locus_tag="KLF07_002333"
     CDS             16720..17742
                     /locus_tag="KLF07_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9832336.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            17739..18635
                     /locus_tag="KLF07_002334"
     CDS             17739..18635
                     /locus_tag="KLF07_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBD9832337.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18632..19618
                     /locus_tag="KLF07_002335"
     CDS             18632..19618
                     /locus_tag="KLF07_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9832338.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            19647..20537
                     /locus_tag="KLF07_002336"
     CDS             19647..20537
                     /locus_tag="KLF07_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9832339.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(20593..21024)
                     /gene="osmC"
                     /locus_tag="KLF07_002337"
     CDS             complement(20593..21024)
                     /gene="osmC"
                     /locus_tag="KLF07_002337"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBD9832340.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5545 a         5190 c         5276 g         5382 t
ORIGIN      
        1 ctgtacggag atataaataa atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg
       61 ctgatgtggc gcaggccgtc gatgacaaaa acatgtacat gcattttttt gaagagatga
      121 cggtctatgc tcctgtccct gtacccgtaa acggcaacac gcattacacc agtgaaagca
      181 tcgagcgttt accgaccggg aatggcaata tcagcgatct gctgagaacc aaccctgcgg
      241 tacgcatgga ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc cctgagaaaa
      301 tctctattca cggtgcgtcg ccctaccaga atgcctattt gattgacggt attagtgcaa
      361 ctaataacct gaacccagcg aatgagtccg atgccagtag tgcaaccaat attagcggga
      421 tgtcacaggg gtattatctt gatgtcagct tactggacaa tgtgacgctt tatgacagtt
      481 ttgtgccggt tgaatttggt cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct
      541 tcaacgctga tgatagcaag gtgaaattgg gttatcgcac tacgcgttcg gactggttaa
      601 catcgcatat cgatgagaat aacaagagcg catttaatca aggctcttca ggaagtactt
      661 attactcccc agattttaaa aagaactttt ataccttgtc gtttaatcag gaactcgctg
      721 ataacttcgg cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt
      781 atgtttcgaa tgacggcatt gtcaccggtc gggcacagta taaaaacgtt atcgatactg
      841 cattgagcaa atttacctgg tttgccagcg accgctttac ccacgattta accttaaaat
      901 ataccggctc cagccgtgat tataatacca gcaccttccc gcagtctgat cgcgaaatgg
      961 gtaataaatc ctatggtctg gcatgggata tggatacgca actcgcatgg gccaaactac
     1021 gtaccaccgt tggttgggat catattagtg attatacccg tcacgatcat gacatctggt
     1081 acaccgaact ttcatgtaca tatggtgata ttacagggcg ttgtacctgt ggcggattag
     1141 gacacatttc ccaggctgta gataattaca ccttcaaaac acgcctggac tggcaaaaat
     1201 tcgccgtggg tgatgtttcg catcaaccct acttcggcgc ggaatacatc tattccgatg
     1261 catggactga acgccataac cagtctgaat cctatgtgat taatgctgcc ggaaagaaaa
     1321 ctaaccatac catttaccat aaaggtaaag gcagcctggg aattgacaac tacacgctgt
     1381 atatggcgga gcacattagc tggcggaatg tgtcgttaat gcccggtgtg cgttatgact
     1441 atgacaacta tctgtcaaac cacaatatct ccccgcgctt tatgacggaa tgggatattt
     1501 ttgctgatca aacctcaatg attaccgccg gttataaccg ttactatggc gggaatattc
     1561 ttgatatggg attacgtgat atccgcaata gctggacgga atcggtatca ggtaataaaa
     1621 ctctgacgcg ttatcaggat ttgaaaacgc cttataacga tgaactggca atgggattgc
     1681 agcagaaaat cggtaagaac gttattgcgc gtgcaaacta tgtttaccgt gaagcgcatg
     1741 atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata
     1801 acaacgatgg caaaaccaaa acgcattcgt tcagcctcag ttttgaactg gccgaacccc
     1861 tgcatatccg ccaagtagat attaacccac aaattgtctt tagctatatc aagagcaagg
     1921 gcaacttgtc gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt
     1981 ataacggtaa tctggtctct tacgatagtg ttccagtggc agattttaat aacccattaa
     2041 agatctcctt aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc
     2101 tggcctggca agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca
     2161 tcagcgaata ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct
     2221 gggacacccg cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg
     2281 ccgatattct caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg
     2341 cgacctacgc cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg
     2401 gattttactt aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct
     2461 ctgtttctta ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc
     2521 cttaccgcag gatgaaaagt taattaccgg gcaactggac aatggcttgc gatatatgat
     2581 ttatccgcat gctcaaccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc
     2641 attgcaggaa gaagacaatg agcgcggcgt ggctcatttt gtagaacata tgatgtttaa
     2701 cggcacaaaa acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg
     2761 tttcggtcgc gatgttaatg cctataccag ctatgacgaa acgctgtatc aggtgagtct
     2821 gccgactacg cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa
     2881 tgccgcaacc tttgaaatac tcgaagtaga cgctgaacgc ggcgtaatta ctgaggaatg
     2941 gcgtgcccat caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc
     3001 aaatacccgt aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac
     3061 accggcacaa ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat
     3121 cgtggtcggc gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa
     3181 gcttccggct aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct
     3241 gcgctttaat atcattaatg ataaagaaaa ccgagtgaac ggcatcgcac tctattatcg
     3301 cctgccaatg gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat
     3361 gttagttcag ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac
     3421 tatttctggc ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt
     3481 ccgtgtaaat gcacgagacg ataatatgca ggatgctgca aatgcattaa tggcagagtt
     3541 ggcaaccatt gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg
     3601 cctcacctgg ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac
     3661 cagtcgcctg gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata
     3721 tcaactttcg aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca
     3781 gcagttaaga aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc
     3841 caaaaaagca ttgtctcttg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa
     3901 gctggcggct tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca
     3961 ggcggaaatt agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa
     4021 tggtgccagg gttattctgg caaaatccgc gggtgaagag caaaagctac aaattactgc
     4081 cgtatctaat aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc
     4141 aaataaagca gtaagcggaa gcggcgttgg tgagctctcc tcttccagcc tgaaacgctg
     4201 gagtgcggaa aattcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc
     4261 cgttagcgcg cggactaata accctgaacc tggttttcag ttgattaacc agcgaatcac
     4321 ccacagcacg attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt
     4381 gaaaacgctc gaccagcgtc cagcggagaa attcgcccag cagatgtatg agacgcgcta
     4441 tgctgatgac cgcacgaaat tactgcaaga aaatcagatt gtacagttta ctgccgcaga
     4501 tgcgctggct gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt
     4561 cggtaatgtc tcagaagaca aactcgtggc gttaattacg cgttacttag gatcaatcaa
     4621 acactctgat tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc
     4681 ggttactgta aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga
     4741 ttcccggaca cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact
     4801 ggcaaaagat ctacgcgtta atattcgtga acaggcatct ggagcataca gcgtttcttc
     4861 tcgcctctcg gttgatcctc aggccaaaga tatcagtcat ttgctggctt ttacttgtca
     4921 accagaacga catgatgaac tgttaacgtt agcgaatgaa gtgatggtta agcgtctggc
     4981 taaagggatc agtgagcaag aactgaatga ataccagcaa aacgttcagc gcagcctcga
     5041 tatccaacag cgtagcgttc aacaattagc gaacactatt gtaaatagtc ttattcaata
     5101 tgacgatcct gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa
     5161 tgttaacact gccgttaaac aatatctttc tcatccggta aatacttata ctggagtatt
     5221 attgccaaaa taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg
     5281 ccatttttat gtaataattt tataaatgcg ttcaaaataa taaccaggta ctaatagtga
     5341 tattttaagg tctgattttt acgtgaaaat tcaggaggca cagaatgcgc ataaaaataa
     5401 cagcataaaa caccttacca ccacccaaga atttcatatt atattgtttt tcaatgaaaa
     5461 aatattattc tcgtaatatc tcacgataaa taacattagg attttgttat ttaaacacga
     5521 gtcctttgca cttgcttact ttatcgataa atcctacttt tttaatgcta tccaatcatt
     5581 ttaaggagtt taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt
     5641 cacgttttgg tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg
     5701 aaatgcgcga cgatgtcgca ttccagatta tcaatgacga attatatctt gatggcaacg
     5761 ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaatgtc cacaaattga
     5821 tggatttatc cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca
     5881 tcgacctgcg ttgcgtaaat atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg
     5941 gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg
     6001 cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa
     6061 acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac tgggatgtgg
     6121 agctgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg
     6181 aagcctgcga cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta
     6241 actatgagtt cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta
     6301 tcgacatcga catgcacatc gacgccgcca gcggtggctt cctggcaccg ttcgtcgccc
     6361 cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccaca
     6421 aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc
     6481 cgcaggaact ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca
     6541 acttctcccg cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc
     6601 gtgaaggcta taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg
     6661 agatcgccaa actggggccg tatgagttca tctgtgccgg tcgcccggac gaaggcatcc
     6721 cggcggtttg cttcaaactg aaagagggtg aagatccggg atacaccctg tacgacctct
     6781 ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca
     6841 ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac
     6901 tgttgctgga agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg
     6961 gtattgccca gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc
     7021 gaaacgaacc cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa
     7081 tattgttctg aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta
     7141 gggggataat atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg
     7201 cttttttgcc ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac
     7261 atcgggcttt tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt
     7321 gggactttgt gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc
     7381 ctgggtatca aatactctgg ggccgagatg gggatttgca gcgatctcat ttggctatct
     7441 gcaaatcgcc attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat
     7501 cctgaaatgg ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat
     7561 tctttgggcg ctggcattaa cgcagtttgg tggcacgaaa tacacggcac gaattgctaa
     7621 agttggcttc ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat
     7681 ttatctgcac tccggtgccc ccgttgctat cgaaatggat tcaaagacct tcttccctga
     7741 cttctctaaa gtgggcaccc tggttgtatt tgttgccttc attttgagtt atatgggcgt
     7801 agaagcatcc gcaacccacg tcaatgaaat gagcaaccca gggcgcgact atccgttggc
     7861 tatgttactg ctgatggtag cggcaatctg cttaagctct gttggtggtt tgtctattgc
     7921 gatggtcatt ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt
     7981 tctgatgtcc catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct
     8041 gttgctgggt gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta
     8101 tgtcacagcg cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt
     8161 accggtaacg ctggtcattt cacagctggt gattacttct atcgcgttga tcatcctcac
     8221 caataccggt ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat
     8281 ttatctgtgt gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc
     8341 tgacttaaaa cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggctat
     8401 tgtcggtctg ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat
     8461 ccagggtgac tctaccgata tgtatgttga attactggtc gttagtttcc tggtggtact
     8521 tgccctgccc tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt
     8581 cactctggag ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg
     8641 tgcacgttca ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg
     8701 ttataagaca agggagcgat aattcatcgc tccctttttc gtgctcgctg tcgtattgac
     8761 ttaaccggga taagtacgag aatgagcgca catctgttta ccggaaacca gcatatatgg
     8821 atatctgctc ccgaaacgag aaattagcga ttagaagacc agcgatactg gttgcactgg
     8881 cacttttact gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag
     8941 gttcaaagcc accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg
     9001 ccacggtgtc tcgcctcgac tggccaccgg tttcctcggt taacattagt aaccccacca
     9061 gccgggcccg tgtacaacaa caggcgatga tcgacaaact ggatcatctg caacgtctcg
     9121 gtataaacac ggtctttttc caggttaagc cggacggtac cgccctgtgg ccatcgaaaa
     9181 ttttgccatg gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc
     9241 tgcaatttat gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc
     9301 cctatcgcgt atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt
     9361 ctcaacaacc ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc
     9421 gctttgtcct cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgcag
     9481 aagtggtttc ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgagt
     9541 caccgggttc acggctaaat gataacgaaa cgtaccgcaa atacggaggc gcatttgcgt
     9601 caaaagcaga ctggcggcgc aacaatactc agcagttaat tgcaaaagta tcgcacacca
     9661 ttaaaagcat taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc
     9721 gatcacacga tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg
     9781 ctgacacccg tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact
     9841 ggccgttctc acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg
     9901 ttaaaccgac taggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt
     9961 caaagataga gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg
    10021 atcttaacga tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga
    10081 ataaaccgca gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaactc
    10141 ccatgcttac tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat
    10201 atcccactag taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt
    10261 taaacagcta taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct
    10321 gtcaccgaaa aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa
    10381 actgtcctaa gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc
    10441 agttagtcaa tctcgcacgt tgcgttttga ttccgtctga tttcccagcc ttataagggt
    10501 gtgaattatg gagatgtatt ttaaaagaat gaaagatgag tggaccggac ttgtcgaaca
    10561 ggcagatcca ctcattcgtg ctaaagccgc ggaaattgcc gttgcgcatg ctcattatct
    10621 gagtatcgag ttttatcgaa ttgtccgcat cgacccgcat gccgaagaat tcttgagtaa
    10681 tgaacaagtt gagcggcagt tgaaaagtgc gatggaacgc tggattatta acgtgctttc
    10741 tgcccaggtt gacgatgtcg aaaggctaat acaaatccag cataccgtcg cggaagtgca
    10801 tgcccgcata ggaattccgg tagaaattgt cgagatgggg tttcgggtgc tgaaaaaaat
    10861 cctctatccg gtaatcttct cttcggatta ttccgccgca gaaaaacttc aggtctatca
    10921 tttctcgatt aacagtattg atatcgcgat ggaagtgatg acccgcgcgt ttacctttag
    10981 tgacagtagt gcctcaaagg aagatgaaaa ttatcgtatc ttctcgttac tggaaaacgc
    11041 cgaagaagaa aaagaacggc aaatagcctc aatactttca tgggaaatag atattatcta
    11101 taaaatcctg ctggattctg atttaggcag tagtttgcca ttaagccagg ctgattttgg
    11161 cctgtggttt aaccataaag gtcgacatta ttttagtggc attgccgaag taggccatat
    11221 ctcccgtctc attcaggatt tcgacggtat tttcaatcaa accatgcgta acaccaggaa
    11281 tttgaataac agaagtctgc gggtgaaatt tttattacag ataagaaata ccgtatcgca
    11341 gattattacc ttgctgcgtg aattgtttga agaagtatcg cgccacgaag tcggtatgga
    11401 tgtactaacg aaattactta accgccgttt cctgccgact atcttcaaac gcgaaattgc
    11461 ccatgccaac cggaccggta caccgctgtc agtgctgatt attgacgttg ataaattcaa
    11521 agagatcaac gatacgtggg gccataacac tggcgatgaa attctgcgta aagtctctca
    11581 ggccttttat gacaacgtcc acagtagtga ttatgttttt cgctacgggg gcgatgaatt
    11641 tatcattgtt ttgactgaag cttctgaaaa cgaaacgtta cgtaccgcag aacgtattcg
    11701 cagtcgggtg gagaaaacca aactgaaagc cgcaaacggc gaggatattg ccctctcact
    11761 ttccatcggt gccgccatgt ttaatggtca tcctgactat gagcgcctca ttcaaatagc
    11821 cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac cgtgttgaac tctggaaagc
    11881 cagtctttag atgcgccagg atgcagaggt aatcatgaag ctaaccgatg cggaaaatgc
    11941 cgccgatggc atttttttcc ccgcccttga acaaaatatg atgggcgcgg tgttaattaa
    12001 cgaaaatgat gaagtgatgt ttttcaaccc cgccgcagag aagctctggg ggtacaaacg
    12061 tgaagaagtc attggcaata acattgatat gctgattcca cgggatttgc gtcctgcgca
    12121 tcctgaatac attcgtcata accgtgaagg tggtaaagcg cgcgtcgagg ggatgagtcg
    12181 ggagctgcag ctggagaaaa aagacggcag taaaatctgg acccgttttg cgctatcgaa
    12241 agtgagcgcc gaggggaaag tttattacct ggcgctggta cgggatgcca gcgtagaaat
    12301 ggcgcaaaaa gaacagaccc gacaattgat tattgccgtt gaccatctcg accgaccggt
    12361 gattgtcctc gatccggaac gccatattgt gcagtgcaat cgcgcattta ccgaaatgtt
    12421 tggttactgc attagcgaag ccagcggtat gcagcccgat acactcctga acattcctga
    12481 attccctgcc gataaccgca ttcgtttaca acagttgcta tggaaaaccg cccgcgatca
    12541 ggacgaattt ctgctgttga cacgcaccgg tgaaaaaatc tggattaaag cctctatcag
    12601 cccggtttat gacgtgctcg cgcatctgca gaacctggta atgaccttct cggatatcac
    12661 cgaagaacgg cagatccgcc agcttgaagg caatattctc gccgccatgt gcagcagccc
    12721 gccatttcat gaaatggggg aaatcatttg tcgtaacatc gaatctgttc tcaacgaatc
    12781 gcatgtttcg ctgttcgcac tgcgcaacgg gatgccgata cactgggcgt catcttcgca
    12841 cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc attcgtcagc gtgatggtgc
    12901 gcctgcgggg atcctgcaaa ttaaaacctc gtcaggagca gaaaccagcg cctttatcga
    12961 acgcgtggca gatatcagcc agcatatggc cgcgctggcg ctggaacagg aaaaaagccg
    13021 tcagcatatt gaacaactca tccaatttga tccgatgacc ggtctgccaa atcgcaataa
    13081 cctgcacaat tacctcgatg accttgtcga caaagccgtc tctccggtgg tgtatctcat
    13141 cggtgttgac catattcagg atgtgattga tagccttggc tatgcgtggg ccgatcaggc
    13201 attgctggaa gtggtcaatc gctttcgtga aaaactcaaa ccggatcagt atctctgtcg
    13261 tatcgaaggt acgcagtttg tcctcgtgag cctggaaaac gacgtcagta acattaccca
    13321 aatcgccgat gagctacgga atgtggtcag caagccgata atgattgacg ataaaccatt
    13381 cccgctaacc ttgagtattg gcatcagcta cgacgtgggt aaaaaccgcg attacctgct
    13441 ctccactgct cacaatgcaa tggattatat tcgcaagaat ggcggtaacg gctggcagtt
    13501 cttcagcccg gcgatgaacg aaatggtaaa agagcgtttg gttttaggcg cagcgctgaa
    13561 agaagcgatt agcaataacc aactgaaact ggtttaccag ccgcaaatct tcgcagaaac
    13621 gggtgaactg tacggcatcg aagcccttgc tcgctggcac gatccccagc atggtcatgt
    13681 acccccttca cggtttattc ctctcgcaga agagattggt gaaatcgaaa atattgggcg
    13741 ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg cgtagccaga atattcatat
    13801 cccggcgtta tccgtgaact tgtcggcgct gcactttcgc agtaatcagc tgcctaatca
    13861 ggtgtctgat gcaatgcaag cctggggtat tgacggccac cagctgacgg tagaaatcac
    13921 ggaaagcatg atgatggaac acgataccga aatctttaag cgcattcaga tcctgcgcga
    13981 tatgggcgtg ggcttatcgg tagatgattt tggcacgggc ttttccggat tatcccgctt
    14041 agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagt tttgtcgatc gttgtctgac
    14101 ggaaaaacgc atccttgcct tacttgaagc cattaccagc attgggcaaa gcctcaattt
    14161 aaccgtcgtg gcggaaggcg tcgaaaccaa agagcaattt gagatgctac gcaagatcca
    14221 ctgtcgcgtt attcagggat atttcttttc ccgcccccta cccgccgagg aaattccagg
    14281 ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt cctctcgccc gcactcgcgg
    14341 gttttcattt aacgtgacgc tgtcacttaa atactgtgat ttcagctacg attccgggag
    14401 attccttcct taacttcccc tcttgcacca aaactgcact acgctgcaca aatgttgatc
    14461 aaaagttaaa aaatataact ttaagccagc attctggcgc ttatcccggc atggcatgag
    14521 atctgcataa gcggaaagcg cagcaatttt tgtcttaata caggaaccgt ttatgtcgga
    14581 taccaccgaa ctggttgatt tagccgtgat cttccctgat ctggagatcg aattgaaata
    14641 cgcctgcgct gataacatca caggtaaagc tatttaccag caagcgcgtt gtctgttaca
    14701 caaggatgcg attaccgcgc tggcgaaaag tatcagtatc gcccagctgt caggcttaca
    14761 actggtgatt tacgatgcgt atcgcccaca acaagcacag gcgatgttgt ggcaagcctg
    14821 cccagacccg caatatgttg ttgatgtgac ggtcggttct aatcacagcc gtggcacggc
    14881 gatcgacctg acgcttcgtg atgagcacgg gaacatcctg gatatgggcg cagggttcga
    14941 tgaaatgcac gagcgctccc atgcctatca cccttccgtc ccgcccgccg ctcagcgcaa
    15001 tcgcctgttg ctgaatgcga taatgactgg tggcggtttt gtcggtatct ccagcgaatg
    15061 gtggcacttc gaattacccc aggcagcgag ttaccctctg ctcgctgatc aattctcttg
    15121 ttttatatcc cctggcacac agcacgtcag ttaatcagga aactgtcatg aagagatcga
    15181 tatcgtttcg tcccacattg ctcgcgctcg tccttgccac aactttcccg gttgcgcacg
    15241 ccgccgtacc gaaagatatg ctggtgattg gtaaggccgc cgatccacaa accctcgacc
    15301 cggcggtaac aatagataat aacgactgga cagtgaccta cccgtcttat cagcggctgg
    15361 ttcagtacaa aacggacggt gataaaggct caaccgacgt tgaaggcgat ctggcaagta
    15421 gctggaaagc gtctgacgat caaaaagagt ggacgttcac cctgaaggac aatgctaaat
    15481 ttgccgatgg cacacctgtc actgccgaag cagtaaaact ctcttttgag cggttactaa
    15541 aaatcggcca ggggccagca gaagcatttc ccaaagattt aaagattgat actcccgacg
    15601 aacatacggt gaagtttacc cttagccaac cattcgcacc gttcctctac acgctagcga
    15661 atgacggtgc atccattatc aatccggcgg tcttaaagga acatgcagcg gatgatgctc
    15721 gcggcttcct cgcgcaaaat accgccggtt ccggaccatt tatgctgaaa agcgggcaaa
    15781 aaggtcagca attagttctg gtgccaaatc cgcattaccc cggcaataaa ccgaacttta
    15841 agcgagtatc ggtaaaaatt atcggtgaaa gtgcctcccg tcgcctgcag ctctcccgtg
    15901 gcgacattga cattgccgat gcgctgccgg tggatcaact caacgccctg aagcaggaaa
    15961 ataaagtcaa tgtggcagag tatccgtcac tgcgcgttac ctatctgtat ctgaataaca
    16021 gcaaagcgcc tcttaatcag gcggatctgc gtcgggccat ttcctggtct accgattatc
    16081 agggcatggt taacggcatt ctgagtggta acggaaaaca gatgcgcggc ccgattccgg
    16141 aaggcatgtg gggctacgat gcgacggcaa tgcaatacaa ccatgacgaa acgaaagcca
    16201 aagccgaatg ggataaagtg acgagcaaac ccaccagcct gacgtttctc tactccgata
    16261 acgatccgaa ctgggagcct attgctctgg cgacacaatc cagtctcaac aagctgggca
    16321 tcaatgtgaa gctggaaaag ctggcgaacg ccaccatgcg cgacagagtg ggtaaaggtg
    16381 attacgacat tgcgattggc aactggagtc cggattttgc cgacccgtat atgtttatga
    16441 attactggtt tgagtcagac aaaaaaggtc tgccgggtaa ccgctcgttc tatgaaaaca
    16501 gtgaggtcga taagttactg cgcaatgcgc ttgcgaccac cgaccagacg cagcgtaccc
    16561 gggactacca gcaggcacag aaaatcgtca ttgatgacgc tgcttatgtg tacctgttcc
    16621 agaaaaacta ccaactggcg atgaacaaag aggtgaaagg ctttgtgttc aatcccatgc
    16681 tggaacaggt cttcaatatc aataccatga gtaaataacg tgaccttctg gagtatttta
    16741 cgccaacgct gctgggggct ggtgctcgtg gtggcgggcg tctgcgtgat tacgtttatt
    16801 atctcgcatc tgatccctgg cgatccggcg cggttactgg cgggtgaccg cgccagcgat
    16861 gctatcgtgg aaaatattcg ccagcaactg ggactggacc agccactgta tgtacagttt
    16921 taccgctact tcagcgacct gtttcatggt gacctgggaa catccattcg taccgggcgt
    16981 ccggtgctgg aagagttgcg tatatttttc ccggcgacgc tggaactggc tttttgtgcc
    17041 ctgctgctgg cactcctgat tggcatcccg ctgggcatac tctctgcagt ctggcgaaat
    17101 cgctggctgg atcatctggt gcgaataatg gccattaccg gaatctccac acctgcgttc
    17161 tggcttggac tgggcgtcat tgtgctgttt tatggtcatc tgcaaattct tcccggcggc
    17221 ggaaggcttg atgactggct ggatccacca acgcacgtta ccggctttta tctgctcgat
    17281 gcgctgcttg aaggcaacgg tgaagtcttc ttcaatgcgt tgcaacatct catcttaccg
    17341 gcattaacgc tggcgttcgt tcacctggga attgtcgctc gccagatccg ctcagcgatg
    17401 ctggaacaat tgagtgaaga ctacattcgt accgcccggg ccagcggctt gcccggctgg
    17461 tatatcgttt tatgttatgc gctacccaat gcgttgatcc catcgattac cgtattgggt
    17521 ctggcgctgg gcgatttgtt gtatggcgca gtgctcaccg aaaccgtttt tgcctggccc
    17581 ggaatgggtg catgggtagt aacatcaata caggcgctcg acttcccggc agtgatgggc
    17641 tttgccgtcg tggtttcatt tgcttatgtg ctggtcaacc tggtggtgga tttgctctat
    17701 ttgtggattg atccgcgtat cggacgtgga ggtggtgaat gatgctaagc gaggaaacgt
    17761 ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc aaaactggta tggatgctga
    17821 aaggcagtcc gctcaccgtg accggcgcag tcatcattgt attaatgcta ttgatgatga
    17881 ttttttcacc gtggcttgcg acgcatgatc ccaacgccat tgatttaacc gcccgccttt
    17941 tgccgccttc tgcggcgcac tggtttggca ccgatgaagt gggacgcgat ctgttcagcc
    18001 gcgtactggt cggcagtcag caatcaattc tcgccggatt agtcgtggtt gccattgcgg
    18061 gtatgattgg ttcgctactc ggatgtctat ccggtgtgct tggcggacgc gcagacgcca
    18121 tcatcatgcg catcatggac attatgctgt cgattccttc gttggtactg acaatggcac
    18181 tggctgccgc tctcgggccg agtttattta acgccatgct ggcgattgct attgtgcgaa
    18241 ttccctttta tgtgcgcctg gcgcgggggc aagcattagt tgtacgccag tatacctatg
    18301 ttcaggcggc gaaaaccttt ggtgcgtcgc gttggcatct gatcagctgg catattttac
    18361 gtaactccct gccgccgctg atcgtgcagg catcgctgga tatcggtagc gcgattttaa
    18421 tggccgccac gttgggattt attggcctgg gtgctcaaca accgagtgct gaatggggcg
    18481 cgatggtagc taatggtcgc aactatgtac tcgatcagtg gtggtattgc gcatttccgg
    18541 gggcagcgat tttgcttacc gccgtcgggt ttaatctctt tggtgatggt attcgcgatc
    18601 tgcttgaccc gaaagcagga ggaaagcagt catgacccaa cccgttctgg acattcaaca
    18661 gctgcatttg agtttccccg gttttaacgg ttatgttcac gtgctcaaca atgtgtcctt
    18721 gcagattaac cgcggtgaaa ttgtcggtct ggtgggagaa tccggctcag gtaaatcagt
    18781 caccgcaatg ctgattatgc gtctgctacc gacgggcagt tattgcgtac atcgggggca
    18841 gatttcactg ctgggagacg atgttcttaa cgctcgggag aaacagcttc gtcagtggcg
    18901 cggcgcacga gtggcgatga tctttcagga accgatgacc gccctcaatc cgacacgtcg
    18961 aataggtctt cagatgatgg acgtgatccg ccatcatcaa ccaataagtc gtcgggaagc
    19021 cagagctaaa gcgattaccc tgctggaaga gatgcaaatc ccggatgccg tggaagttat
    19081 gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag cgggtaatga ttgcgctggc
    19141 attctcctgc gagccgcaat tgattattgc cgacgaaccg actacggcgc tggacgtcac
    19201 ggtacagttg caggtactgc gtttgcttaa acataaagct cgcgccagtg gaactgcggt
    19261 actgttcatc agccatgata tggccgtggt gtcgcaactg tgcgatagcg tttacgtgat
    19321 gtatgccgga agcgtaattg aaagcggcgt gacggcagac gttatccatc atccccggca
    19381 tccgtatacc attggtttgc tgcaatgcgc accagaacat ggagtaccac gccagccatt
    19441 acccgccatt ccagggacgg taccaaacct cacccatttg cctgacggct gcgcttttcg
    19501 cgatcgttgc tatgcggcag gtgcacagtg tgaaaacgtc ccggcgctga cagcgtgtgg
    19561 tgacaacaac cagcgctgcg cctgttggta tccgcagcag gaggtcataa gtatctgaca
    19621 cgttattaac gttacgcgac gtccatatca atttcccggc ccgtaaaagc tggcttggta
    19681 aaactacgga acatgttcat gcgattaatg gtattgattt acagatccgc cgtggtgaaa
    19741 ccttagggat cgtcggtgag tcaggctgcg gcaaaagcac cctcgcacag cttttaatgg
    19801 gtatgctgca accgagccac gggcaataca tccgttcagg atcacaacgc attatgcaga
    19861 tggtgtttca ggacccgctc tcttcgctta atccgcgctt atcggtgtgg cgcatcatca
    19921 cagaaccgct ctggatagct aagcgtagta gtgaacaaca gcggcgagcg ttggcaaagg
    19981 agttggctgt gcaggtgggt attcgtccgg agtatctcga ccgcctgcct catgcgttct
    20041 ccggcgggca gcggcaacgc atcgccattg ccagagcact ctcttcgcag cctgacgtga
    20101 ttgtgcttga tgagccaacc tcggcgctgg atatctccgt gcaggcgcag atcctcaatt
    20161 tactggtaac gctacaggaa aatcacgggc tgacttatgt gctgatttca cacaatgtct
    20221 cggtgatacg tcatatgagc gatcgggtgg cggtgatgta tctcgggcag attgtagaac
    20281 tgggcgacgc gcagcaggtg ctgacggcac ctgcacatcc atacacccga ttattgctgg
    20341 attccctccc cgccattgat aaaccgctgg aggaagaatg ggcattatgt aaaacggatc
    20401 tgccgggaaa ccgcacgttg ccacaaggct gttttttcta cgaacgttgc cctctggcaa
    20461 cccacggatg tgaagtccgg caatcattag caataaggga ggacggacgt gagctccggt
    20521 gctggcgggc gctgtagaga tgctaacaac gcatcaggca ttcacgcgcc tgacgcgtca
    20581 tccggcaatg ctttacgatt tcaactggta atccagcgta atttccgctt tcagtacctg
    20641 agaaaccggg catcctgctt ttgctttctg gatgatgccg tcaaaggtag aggcatcaat
    20701 acccggcacc gcaacttcac tcttcagtgc gattttcgta atcgcaaaat cggcatccac
    20761 tttatccagc gacacatcgg cggtggtatc aatcgatgtt ggcgtgaatc ccgcttcccc
    20821 cagcattaat gaaagcgcca ttgagaaaca tgcggcatgc gctgcgccaa tcagttcttc
    20881 agggttggtt cctttttcgc cttcaaaacg cgtgttaaat ccatacggct gttggttcag
    20941 cacgccactc tgggtggata ctgttccctt cccgcgtttg atatcgcctt cccagtgtgc
    21001 ctgacctttc ttatggattg tcattgttgc tctcctgtgg gcttaaaaag gaaattatag
    21061 actacgtgag aaacagcacg aggataagca ggatattccg aataaaatac ggcgaaaata
    21121 ttacttctgc acttaaatta aattcccaca atagataacc gggaatatat atctcaagag
    21181 atattgcatc aatatcaatc aactcaccac aataataata catatcaata tcatggagtt
    21241 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
    21301 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
    21361 gaatcactat gtttacttat tatcaggcag aaa
//
LOCUS       DAEEBB010000067        21313 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEEBB010000067 DAEEBB010000000
VERSION     DAEEBB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.067"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="KLF07_002338"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="KLF07_002338"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBD9832341.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2729)
                     /locus_tag="KLF07_002339"
     CDS             complement(1671..2729)
                     /locus_tag="KLF07_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD9832342.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="KLF07_002340"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="KLF07_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="KLF07_002341"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="KLF07_002341"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBD9832343.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="KLF07_002342"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="KLF07_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBD9832344.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="KLF07_002343"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="KLF07_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBD9832345.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7686..8300
                     /gene="evgA"
                     /locus_tag="KLF07_002344"
     CDS             7686..8300
                     /gene="evgA"
                     /locus_tag="KLF07_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBD9832346.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8305..11898
                     /gene="evgS"
                     /locus_tag="KLF07_002345"
     CDS             8305..11898
                     /gene="evgS"
                     /locus_tag="KLF07_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBD9832347.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="KLF07_002346"
     CDS             complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="KLF07_002346"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBD9832348.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="KLF07_002347"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="KLF07_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBD9832349.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="KLF07_002348"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="KLF07_002348"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBD9832350.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="KLF07_002349"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="KLF07_002349"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBD9832351.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="KLF07_002350"
     CDS             complement(17698..18321)
                     /locus_tag="KLF07_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBD9832352.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /gene="ypdI"
                     /locus_tag="KLF07_002351"
     CDS             18618..18893
                     /gene="ypdI"
                     /locus_tag="KLF07_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBD9832353.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="KLF07_002352"
     CDS             complement(18970..19212)
                     /locus_tag="KLF07_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBD9832354.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="KLF07_002353"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="KLF07_002353"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBD9832355.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="KLF07_002354"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="KLF07_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBD9832356.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..>21313)
                     /locus_tag="KLF07_002355"
     CDS             complement(20977..>21313)
                     /locus_tag="KLF07_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD9832357.1"
                     /translation="QYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKIPEPYAAMGSLE
                     FAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKAMFRADGLLPAS
                     SKHIHENAE"
BASE COUNT         6067 a         4920 c         4592 g         5734 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     7501 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     7561 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     7621 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     7681 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     7741 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     7801 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     7861 acggtatcct ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     7921 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     7981 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     8041 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     8101 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     8161 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacttata
     8221 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac
     8281 aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt
     8341 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     8401 agtaacaacc gtgtcatact tgatccagta cgtctgagca acaaggaatt acgttggtta
     8461 gcgagcaaaa aaaatcttgt gattgcagta cataagtccc aaacggctac gttgttgcat
     8521 accgattcgc agcaacgagt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     8581 acgttaaata tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg
     8641 ctggaagaag gtgaagtcga tatagtgtta tcacatttag tagcttcgcc gcctcttaat
     8701 gatgacattg ctgcaaccaa accactgata attacctttc cggcgctggt aaccaccctt
     8761 cacgattcaa tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat
     8821 tatccccctg acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     8881 aatttatatc aggcattagc atccgtctca gccggacaga atgattactt tattggtagt
     8941 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     9001 tattataact caccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcgttctt
     9061 aatgaagtac tcaatcgatt tgttgatgct ttgacaaatg aagttcgcta tgaagtatca
     9121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     9181 catgaaaaac aatggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcc
     9241 cctccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt
     9301 aatattatta ctttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     9361 catgctggaa cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt
     9421 gaagatagag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     9481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     9541 ccatattatt atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata
     9601 aaagtcgata acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg
     9661 gtcgcgacac agttaaattc acgttacatg atcgaccatt actatcctaa tgaactttat
     9721 cattttctta ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     9781 ccggaactta aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg
     9841 cgcctgaccg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat
     9901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     9961 tggggatttt acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
    10021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
    10081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
    10141 tactacaaaa atgcaatgtt accattagaa aacagtgact ccccctttaa agatgttttt
    10201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggtt
    10261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
    10321 ctgccagcca gcgatcatgc tgtttatatt tgtggttggc aagatattac agagacgcgt
    10381 gatttaattc atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa
    10441 agtcagtttc tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc
    10501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
    10561 cttgcctact ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
    10621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
    10681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaatttgc
    10741 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
    10801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
    10861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
    10921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
    10981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
    11041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
    11101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
    11161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
    11221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
    11281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
    11341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
    11401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
    11461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
    11521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
    11581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
    11641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
    11701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
    11761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
    11821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
    11881 tgtcagaaaa atgactaaat agcggctccc acaatgttct aatgtgggag ctatttacca
    11941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
    12001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
    12061 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
    12121 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
    12181 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
    12241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
    12301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
    12361 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
    12421 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
    12481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
    12541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
    12601 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
    12661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
    12721 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggtcgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagcggct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aagagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgcagca gggtcgatga ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgaa tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tatttattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac tggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga caaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ctaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg tttatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattaa cgccgtcttg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg
    21301 gcgtttgtac tgt
//
LOCUS       DAEEBB010000068        21003 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEEBB010000068 DAEEBB010000000
VERSION     DAEEBB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.068"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..170)
                     /locus_tag="KLF07_002356"
     CDS             complement(<1..170)
                     /locus_tag="KLF07_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832358.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="KLF07_002357"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="KLF07_002357"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBD9832359.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="KLF07_002358"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="KLF07_002358"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBD9832360.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="KLF07_002359"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="KLF07_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBD9832361.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="KLF07_002360"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="KLF07_002360"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBD9832362.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="KLF07_002361"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="KLF07_002361"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBD9832363.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="KLF07_002362"
     CDS             4288..5151
                     /locus_tag="KLF07_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBD9832364.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="KLF07_002363"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="KLF07_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBD9832365.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="KLF07_002364"
     CDS             6489..7106
                     /locus_tag="KLF07_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBD9832366.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="KLF07_002365"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="KLF07_002365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBD9832367.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="KLF07_002366"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="KLF07_002366"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBD9832368.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="KLF07_002367"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="KLF07_002367"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBD9832369.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="KLF07_002368"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="KLF07_002368"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBD9832370.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="KLF07_002369"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="KLF07_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBD9832371.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="KLF07_002370"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="KLF07_002370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBD9832372.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="KLF07_002371"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="KLF07_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBD9832373.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="KLF07_002372"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="KLF07_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBD9832374.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="KLF07_002373"
     CDS             18072..19781
                     /locus_tag="KLF07_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD9832375.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="KLF07_002374"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="KLF07_002374"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBD9832376.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5713 c         5385 g         4822 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccacg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAEEBB010000069        20711 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEEBB010000069 DAEEBB010000000
VERSION     DAEEBB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.069"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="KLF07_002375"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="KLF07_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBD9832377.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="KLF07_002376"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="KLF07_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBD9832378.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="KLF07_002377"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="KLF07_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBD9832379.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="KLF07_002378"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="KLF07_002378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBD9832380.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="KLF07_002379"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="KLF07_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBD9832381.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="KLF07_002380"
     CDS             complement(8214..9107)
                     /locus_tag="KLF07_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBD9832382.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="KLF07_002381"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="KLF07_002381"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBD9832383.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="KLF07_002382"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="KLF07_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBD9832384.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="KLF07_002383"
     CDS             complement(10238..11470)
                     /locus_tag="KLF07_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBD9832385.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="KLF07_002384"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="KLF07_002384"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBD9832386.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="KLF07_002385"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="KLF07_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBD9832387.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="KLF07_002386"
     CDS             complement(14275..16539)
                     /locus_tag="KLF07_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBD9832388.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="KLF07_002387"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="KLF07_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBD9832389.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="KLF07_002388"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="KLF07_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBD9832390.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="KLF07_002389"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="KLF07_002389"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBD9832391.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="KLF07_002390"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="KLF07_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBD9832392.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAEEBB010000070        20650 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEEBB010000070 DAEEBB010000000
VERSION     DAEEBB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.070"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(110..1312)
                     /locus_tag="KLF07_002391"
     CDS             complement(110..1312)
                     /locus_tag="KLF07_002391"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBD9832393.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(1322..2134)
                     /gene="hcaB"
                     /locus_tag="KLF07_002392"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1322..2134)
                     /gene="hcaB"
                     /locus_tag="KLF07_002392"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBD9832394.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(2131..2451)
                     /locus_tag="KLF07_002393"
     CDS             complement(2131..2451)
                     /locus_tag="KLF07_002393"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBD9832395.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(2451..2969)
                     /locus_tag="KLF07_002394"
     CDS             complement(2451..2969)
                     /locus_tag="KLF07_002394"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBD9832396.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2966..4327)
                     /locus_tag="KLF07_002395"
     CDS             complement(2966..4327)
                     /locus_tag="KLF07_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBD9832397.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4463..5353
                     /gene="hcaR"
                     /locus_tag="KLF07_002396"
     CDS             4463..5353
                     /gene="hcaR"
                     /locus_tag="KLF07_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBD9832398.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5513..6652
                     /locus_tag="KLF07_002397"
     CDS             5513..6652
                     /locus_tag="KLF07_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBD9832399.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6644..7924)
                     /gene="csiE"
                     /locus_tag="KLF07_002398"
     CDS             complement(6644..7924)
                     /gene="csiE"
                     /locus_tag="KLF07_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBD9832400.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(8115..8969)
                     /locus_tag="KLF07_002399"
     CDS             complement(8115..8969)
                     /locus_tag="KLF07_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD9832401.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(9114..9917)
                     /gene="suhB"
                     /locus_tag="KLF07_002400"
     CDS             complement(9114..9917)
                     /gene="suhB"
                     /locus_tag="KLF07_002400"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBD9832402.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10036..10773
                     /gene="trmJ"
                     /locus_tag="KLF07_002401"
     CDS             10036..10773
                     /gene="trmJ"
                     /locus_tag="KLF07_002401"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBD9832403.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            11074..11562
                     /gene="iscR"
                     /locus_tag="KLF07_002402"
     CDS             11074..11562
                     /gene="iscR"
                     /locus_tag="KLF07_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBD9832404.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            11803..13017
                     /gene="iscS"
                     /locus_tag="KLF07_002403"
     CDS             11803..13017
                     /gene="iscS"
                     /locus_tag="KLF07_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBD9832405.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            13045..13431
                     /gene="iscU"
                     /locus_tag="KLF07_002404"
     CDS             13045..13431
                     /gene="iscU"
                     /locus_tag="KLF07_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBD9832406.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            13448..13771
                     /gene="iscA"
                     /locus_tag="KLF07_002405"
     CDS             13448..13771
                     /gene="iscA"
                     /locus_tag="KLF07_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBD9832407.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13867..14382
                     /gene="hscB"
                     /locus_tag="KLF07_002406"
     CDS             13867..14382
                     /gene="hscB"
                     /locus_tag="KLF07_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBD9832408.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            14399..16249
                     /gene="hscA"
                     /locus_tag="KLF07_002407"
     CDS             14399..16249
                     /gene="hscA"
                     /locus_tag="KLF07_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBD9832409.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            16251..16586
                     /gene="fdx"
                     /locus_tag="KLF07_002408"
     CDS             16251..16586
                     /gene="fdx"
                     /locus_tag="KLF07_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBD9832410.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16598..16798
                     /gene="iscX"
                     /locus_tag="KLF07_002409"
     CDS             16598..16798
                     /gene="iscX"
                     /locus_tag="KLF07_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBD9832411.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16976..18259
                     /gene="pepB"
                     /locus_tag="KLF07_002410"
     CDS             16976..18259
                     /gene="pepB"
                     /locus_tag="KLF07_002410"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBD9832412.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            18401..19177
                     /gene="sseB"
                     /locus_tag="KLF07_002411"
     CDS             18401..19177
                     /gene="sseB"
                     /locus_tag="KLF07_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBD9832413.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19670..20515)
                     /gene="sseA"
                     /locus_tag="KLF07_002412"
     CDS             complement(19670..20515)
                     /gene="sseA"
                     /locus_tag="KLF07_002412"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBD9832414.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
BASE COUNT         4903 a         5249 c         5625 g         4873 t
ORIGIN      
        1 gtagtgtgtt cataaccaat ccctttgcaa gataatgtga tttaaacgta atacacactc
       61 ttgcacctcg ccacgcccca ggcgttgaga tattcgctaa ttatcctggt tacagtgatt
      121 taagcgcgat gttctcatct atcagcagtt tcgcatcaaa cgttttgccg ctctggatcc
      181 atttgcgaat tgggcgaatc tcacgcccct gattcagcgt gaccgcaccg ataagcacgc
      241 cgttttgcag attaaaccaa atcgccttct gagtttccgg gttgccacga caaagccagt
      301 catcgccacg catatcgcca ataaactgta agttatcact gtactgatcg ctccagaacc
      361 acggcggcgg cagtagcggt agcggtagcc ctaacattgc ggcagcggca atttgcgcct
      421 ggttattggc gttttcccag ctttcgcagc ggtgtagtgc accattatca agacgagtga
      481 ttgccacatc gccaccggca aagatcgcgg gatcgcaggt gcggcaagcc tcatcaatga
      541 caatgccatt ggcagtatca aggttggcct cgcgagccag ttgctcgttg gcgctgatac
      601 caataccgta aatcaccaca tcagcctgaa gcgtttcccc actttgcagc gtcagttcta
      661 ctttttcacc atcgaccaca tgttcaatgg cattattgag cagaatgcgc acaccagcct
      721 gctggtggcg ctgtaaaaga tagcgttgca cgggcggtgg tgcattacgg cccatgacgg
      781 ttgccgccag ttcaatcact gtcaccttac atctgcgctg cgtggcgctg gcagccagtt
      841 ccagaccaat agttccggca ccgacaatca cgactgaccg ttcgggctgc agaacttctc
      901 gcagtctggc ggcatcaccg gcatggcgta gggtaaagca gcgttctccc agtgcatcaa
      961 gcaacggcag cggtcgagct gccgcgccga ttgctataaa aagctgatcc cagtgccagc
     1021 tttcgccgtt ggttaacact aactctcgtg tgtcgcgacc cagcgttttg atggttacac
     1081 cggaatgcag atgaacattg ttttcctgcc accagttagc gggtaacacc tgctgtaact
     1141 gcggggaatc ttccagcaac atggatttcg agagtggagg tcgttcataa ggaagatgtc
     1201 gctcatcgga aaacagatgc agctcaccgg tgaacccttg ctggcgtagc gaggccgcag
     1261 ccattgccgc cgcttgcccg ccaccgacaa taatgatcgt tttttctttc atcgtgcttc
     1321 cttaaagatc cagcccagcc gctacgtggc gaatgccgcg aatcgctaaa cccgcatcag
     1381 cgttgatcat cacaccgctt aatgcgcgat tattgcgccg cgatgccaac atcacatacg
     1441 gccccgtaaa atccgccggt tgcgggaaaa attgcagcgg taaaatggcg gcaattttct
     1501 ccggcgtcag agactgcatt atcgaggttt cactttgccc gagcgcctgt gggccgcgca
     1561 ggtcgctggc cataccacac gggccgacgc cattcacccg cactttcggt gccagttcat
     1621 aagccagttg gcgaataagt ccggttgcgg catgtttact ggcggtgtac agcgggccac
     1681 cgccgccagg atacctggcg gcatttgaca gtgtgaaaat catgctgcct tcactggcga
     1741 ttaacgccgg agcgcaggct tttgcgccca gcaggtaacc gagtacgttg acgttaaaca
     1801 gctcgtggaa gccggtttcg agcgtctctg cgggagtatt aaccagtgag gcattgtgat
     1861 cccagatgcc tgcattgccg ataaaacaat ccagcttgcc ggaacgagtc aggatctgat
     1921 cgaccgcgcg ttgataatcg gcataacagg tcacgttacc ttccaccgcc agaatatgtt
     1981 cgccaaatcg ctgacgcaga ctggcgactt ttgccgccga cagttccagc gtggcaactt
     2041 gcgcgccttc ttcgataaat cgctcgacca gcgccagccc taatcccgat ccgccgccgg
     2101 taataaaaat ggactcgtta tgcagatcgc tcatggctgc gcctccggta agtcgatgaa
     2161 aatgtcacca ccttcaacgt gtactggata agtggtgagc ggatcggtgg cgggcaggca
     2221 taacgctttc cccgttttca ggcaaaaact ggcggcgtgt agcgggcact ccaccgtggc
     2281 gtcatcttcc agataccctt ctgacatcga cgcattacca tggctgcaac gatcgttaat
     2341 ggcataaaac tcgccgccaa cgttgaacag ggcgatgacg ggcgaggtat cgatccggag
     2401 agcctcaccc tccggcacat ccgcaacggg acacgcataa attcgattca tcagaacagt
     2461 acactcaggt tatgggaagt gattaccgct tgatccagga cgatatcccg ttccagcaag
     2521 cgccagttgt catcttccag acggcgaact ttgtcgaaac gcgttccaac atagaatgtt
     2581 tcatcgcgct ctttttgtgc ccgataaagc agataattta cccgcacagc aaatacgttt
     2641 gggatgtcgg tttcgcttat ctggcagttg ctgattaagt gacgggtgcg tgacggcggc
     2701 tcttctgccc aggccatgcc cgtttccaga cgggcgattc gccgctccag ctggtctttg
     2761 gtgtcattaa aaatccaggt tgtcggtggc tggacgcctt tgcggcggtc gcgtgtttgc
     2821 gcgttaactg tggtgcgcat ggtgtaacga atctctttgt cgagctgctc cagccagtca
     2881 cgaaatttcc agtcgtccag taagctggct tcgtgaaaga gaaactggct aatgcggtga
     2941 tgtaactcta gtgaaacttg cgcactcatt tcatcacctc ctgctggtaa gcggcggttt
     3001 tatcgagcac ttcctgccag ctttcgctac tcagaagatc ggcccagcgt tggtacattc
     3061 cacgagcggc agtttctgaa aagatatagt tagtaatgcc aggaatgccg tcgtcgcggc
     3121 gcttttcctg accaagcccc atttccagac acagtttgct gttgcgggcg cggtggcctt
     3181 taagcaattt ctggatttca caccagttct ccgagtcatc ctgctcgaga aaaccagcag
     3241 gaccaaaagc acgagtggcg ctgttttcaa aagcggcttt aacttcatcg gaggcggctt
     3301 tgtcagtaat acagaacgcc cacacttcaa cttgatcagg gccgcgcgga tgccagacgc
     3361 ggagcgtggc agtgccgttg agccatgaaa gcgtggggaa aatattgtta tgacccgcca
     3421 ggcgcagggc gcgaacttca cctaaacgtt gttctgcttc ggcataggtt tcgcggtaat
     3481 agcttgaaac tgcgccatcg acccagacat tagcatccgg tttttcagta aagaagaaac
     3541 cgctaccgtg accgtcctga ccaaattgca gcgcatcttt ggcggtttcc cacaccgggc
     3601 gggcggtttg tccatcaccg aggcgcttat cgctgccatc atctttcgcc cctaatacct
     3661 gaacggcaga agcatggctg aacagagcat gatactggtc actggcgaac tgctctgccg
     3721 ggaatttcca gttacagttg atcacccact tttgtacgcc gccgacaatt tcggtgccgc
     3781 cttcgcgacg atccagcatg ccatccagat accaggcaat gtcacccagg taatcacgca
     3841 ggcccggtgc gctggtatcc cagttgccaa aaatcagccc tttataactc tccacacaag
     3901 gaacttcgtt tagtccccag tgggatttac acaacccttg tgggtaggcg cgaggttcca
     3961 gcggtacatc gatcaactcg ccgttaatgc cataagacca gccgtgatac ggacaggtaa
     4021 aggcgcgagt gttgccgcaa tctgcataac tcacacgcat ggcccggtgg cggcattggt
     4081 tgagaaacgc cttaatgctg ccgtctttct gacgcactac gacaaccgca tcttccccca
     4141 tgtaggtgtt aaagaaatca ccaggttttg ggatctggct ttcgtgggcg agaaataacc
     4201 agcaacggcc gaaaatacgt tcaagctcca gttggtaaat atctgggtcg gtataaatac
     4261 gcggagtgac ccgaccattt tgggtatcaa tcagttggta aatgtttaaa tctgagggtg
     4321 tggtcatatt ttttaatcct gaaagataag atgtattgac cttctatttt tgtgtcataa
     4381 ggatgttgtt ggttggttgc taatatgtga aatagttaat ttctaccagc tgagacgttt
     4441 tttcgataac aaggggaagg tgatggaact acggcattta cgctatttcg tcgcagtggc
     4501 gcaggcactg aactttaccc gtgcggcgga aaaactgcat acctcacagc cttcgttaag
     4561 cagccagatc cgcgatcttg aaaactgtgt cggtgttccg ttactggtga gggataagcg
     4621 caaagtcgcg ttgacggcgg cgggagaatg ttttctccag gacgcgctgg caatcctcga
     4681 acaagcggaa aatgccaaat tacgggcgcg gaaaattgtt caggaagaca gacaattaac
     4741 cattggcttt gtgccatcgg cggaagtgaa tttattgcca aaagtattac caatgtttcg
     4801 tctcagacag ccagacacct tgattgagct ggtgagttta atcaccacgc aacaggagga
     4861 aaaaattcgc cgtggtgaac tcgacgtcgg cttaatgcgc catcctgttt atagcccgga
     4921 gattgattat ctggagcttt ttgacgaacc attagtggtg gtgttaccgg ttgatcaccc
     4981 tttagcacat gaaaaagaga tcaccgccgc gcaactggat ggcgtgaatt tcgtcagtac
     5041 cgatccggcg tattccggtt cgcttgcgcc gatcgttaaa gcgtggtttg cgcaagaaaa
     5101 cagccagcca aatatcgtcc aggtggcaac gaatattctg gtgaccatga atctggtggg
     5161 catggggctg ggcgtcactt tgatacccgg ttatatgaat aattttaata ccgggcaggt
     5221 tgtttttcgt cccattgccg gaaatgtgcc ttccattgct ttactgatgg cgtggaagaa
     5281 aggggagatg aagccggcat tacgcgattt catcgccatt gtgcaggaac gtttggcaag
     5341 cgtaacggca taattacgca ttatttttgc aacatgcgtc gcggatcgca gttttttagt
     5401 gcttgtgaac tggcgttaca gacatcggga agcaggctcg aatattctta tttttcaact
     5461 tgcaccaaca ttcgattaac cccagactaa cgcctcctga cgggagggac tcatggtttt
     5521 gcaatccacg cgctggttgg cgctcggcta tttcacatac ttttttagtt acggcatttt
     5581 tctacctttc tggagcgtct ggcttaaagg gattggttta acgccagaaa ccatcggcct
     5641 gttattgggg gcaggtctgg ttgcccgttt tctcgggagt ttgctcatcg cgccccgcgt
     5701 cagcgatcct tcccgcctga tttccgcctt gcgcgtgctg gcactgctga cacttctctt
     5761 tgctgtcgcc ttctgggcgg gggcgcacgt agcgtggctg atgctggtga tgattggctt
     5821 taacctcttt ttctcaccgc tggtaccgtt gaccgatgca ctggcgaata cgtggcaaaa
     5881 gcagttcccg cttgattacg gcaaagtgcg actgtggggc tcggtggcgt ttgtcattgg
     5941 ctcggcgctg acgggcaaac tggtcactat gtttgattat cgggtgatcc tcgcgctgtt
     6001 gacgttgggc gtggcatcca tgctgctcgg ctttctcatc cgtccgacga ttcagccaca
     6061 aggggcaagt cgccagcagg agagcaccgg ttggtctgcg tggttggcgc tggttcgcca
     6121 gaactggcgc tttctggcct gcgtttgttt attgcagggg gcacatgcgg cctattacgg
     6181 ttttagcgcc atttactggc aggcagctgg ctactcggcc tcggcggtgg ggtatttgtg
     6241 gtcgctgggc gtggtggcgg aagtcattat ctttgcgctg agtaataaac ttttccgccg
     6301 ttgtagtgct cgcgatatgc tgttgatctc ggcgatttgc ggcgtagtgc gctggggcat
     6361 tatgggagca actacggcgt tgccgtggtt gatagtggtg caaattttgc attgcggcac
     6421 cttcacggtc tgccacctgg ccgccatgcg ctatattgct gttcgccagg gtagcgaagt
     6481 catccgttta caggcggttt actctgccgt cgcgatgggc ggcagtatcg ctatcatgac
     6541 cgttttcgcc ggtttcctgt atcaatatct gggccacggc gtgttctggg taatggcgct
     6601 ggtggcgctt ccggcaatgt ttttgcgccc gaaagttgtt ccctcatgct gattcgagca
     6661 ttttgcggat ctgctcctgt tgatgtgttg tcagcgtcag gtccgtatag atcagcggtg
     6721 gtgaaaagag cggtaatggc atggtataag gcgcaataat cagtgccgcg ccacgcggag
     6781 cgcctgtctg caaaaatgcc tttaccgaca tatgcttaat gttgagcggc agtaacgtta
     6841 gttcgcgtaa ctgctgctca atctgcgctt ctcgctcgct atcattcccg gtcagtagaa
     6901 taatctgttt ttcatgcaga tcgttttcct gcattagcca ggcaccgaaa atcaccgcga
     6961 ccagaccgct ttcctcatca gaaagatgga cgccgtattc actttcaaat ccggccagcg
     7021 ccgcgcgggt ggtgcgcacc aggcgtgggt acagtctggc gaactcttcc ggcagggtat
     7081 tatcaatacc gatggcaaac aaactgcgat ttaacgcctg ggcgaggtgg gtataaagct
     7141 gatcgcataa cccctgttca tcataaaaac gaacatttcc cagctcacga aaatggttta
     7201 ccagacgttt gatagactga cgcaattgtc tgtctcgctg atgcgcatca cgcaatggat
     7261 cgggaacccg caacatggaa aaaagtaatg ccataaacag tggctcatca ggcggtacag
     7321 gctggagcgc ccgacgctgc cagtggcgtc caatttcctg cgctacctga aattcaaggc
     7381 aggattccgc ccagcgacgt tggagcggat taaactgagg cgtaattcca gcgtggtgtt
     7441 gcagcaaaca atattgcaga tacagacaca ggaaatgaat atcgcgcgtt tcaaagcgtt
     7501 tttgcagccg tcgggagcag agattcacta acgcttgtag attggtatcg tcgtaaaagt
     7561 tacgcgcaat tccgcgccgc ttcagttcac tcttcagggc gggggtgaat tggctggtaa
     7621 taaatgacgg acacagacgg aaaccacgtc gtagccagtg aaataaacac aaacgttggt
     7681 taagcactgt accttcaacc cggtagctac cgtcataacc agtcgtcagt gtgagttgat
     7741 gatagcgcag gatctcccgt cctgtttcgc tgatatcaag actggcaata tcatcatcca
     7801 caccattaag ctcgctgaag gttgccatgg tggcggtcaa ccccggctga aagagcgtca
     7861 gcaagatctg gcagcggcgc tggggagccg aaaggacaga tggtggagca agcgtaggca
     7921 tcatctgtag atctcccata agcataattg ataagaatag ctaaagtttg ctcagggaaa
     7981 tggcgaaggg aatgctaatc atcagaaatg ttggcaagcg tcacagaatt tttctcatct
     8041 taaactgttc tgaccagtgg attagcatgt cttaaggtgg ttttgtgaat gattatcgtg
     8101 ctagcgatca tgggttagtt ctgcggattc aacgcgctaa ggataaagtc caccatctgt
     8161 tcgcgataaa catcgccgtg acgatcggaa aaagcatcaa tgtgttcgcc atccgggatt
     8221 aggatcaacc gttttgtctc ttttgccagg ctatacaact tttcgctgtg ctgccatggg
     8281 ataacgtgat cagctttacc gtgaatgagt aaaagcggga tcgggctgac gctggcgata
     8341 taattttcgc cgctgtaact ctcatcaagt aagtagccac tgccggggat catctgattg
     8401 gcgatggttg cataagaggc aaatgtggag tcgaggatca ccgcacgtat gccttcacga
     8461 tcatcctgac caataacagc aagaatattc gccccgccaa tactctgccc gaacagcacc
     8521 agccgttgtg ggtttacatc actgcgatgg cgcaccacat tgatggcact ttgcgtatcg
     8581 tccagcaatc cggcctggga cggcgtgcct tttgatttac caaacccgcg ataatcaaac
     8641 ataaaaacgt tgaaattacg ctcgggtaac caactgacca gcggccagtg ggcggacata
     8701 tttccggcat tgccgtgagc atgaatgatg gttgcgatgg cattgtcagc agggcccgtc
     8761 gaagaaggaa taaaccagcc ttgcagacga gtaccgtcct tagccgtaaa ttcgacggat
     8821 tccgccgacc aggggtcggg accgtaaatt ttatcgtcag gataatagaa gaagttgatg
     8881 gcaacgcgcg gcactaaata aacgcagaag gcaacaacaa agagaataaa cagaattttg
     8941 agaacgcgtt tgttcactgg cagcgccata acctttcctt gtgacttctt tatcatccat
     9001 taatcgggga aagatggtgg gagagtgacg aaagaaagta aagcggtgag gaagtatttc
     9061 agaatagcgc ctgaaaggcg agggcgggtg agtgacatca cccgcctgag tcattaacgc
     9121 ttcagagcgt cgcttaactc gtcacgcatg ttcgccagca tggctttcac aacgcgcggg
     9181 ttaccagcaa cgatgttacc ggtcagcatg tagttatgac caccggtgaa gtcgctgacg
     9241 atgccgcctg cttcacgaac cagcagctcg cctgccgcga aatcccacgg acgcagaccg
     9301 atttcaaaga aaccgtcaac acgacccgca gcgacgtaag ccagatccag cgccgcagaa
     9361 ccggtacgac ggaagtctgc acattcgttg aacagtttgc cgacgatgtt gatgtaggta
     9421 gtggcgtact gttttgcttt gaacgggaag ccggtcgcca gaatggtacc gtcgagatcg
     9481 cgagcggtgc taccgcgcag acggtagccg ttcagctgtg cgccctgacc gcgagtggcg
     9541 gtgaacagtt cgttacgcat aggatcgtat accacagcaa cttcggtgcg gcctttgata
     9601 cgaacggcga tagataccgc gaagtgcggc agacgtttga taaagttggt agtgccatcc
     9661 agtggatcga taacccattg aacatcctga tcagtacctt caagttcacc gctttcttcg
     9721 gtgatgatgg tgtgctgtga gtaagattta cgaatcgtgt cgataatcac cgcttcggca
     9781 gctttatcta cgttggtcac gaaatcgtta ctgcctttct ggctcgcttc tacagcgtcc
     9841 ggggtttcat agtttttggc aattaaatta cccgccttgc gcgctgcgcg cacggcgatg
     9901 ttcagcatcg gatgcatcgg tctctctcac tggatgttaa agaacgggaa aacggcgcag
     9961 agtatagcag cgagatcggc atctgtcttc gttttatgat aagatgcgtg aataatcttt
    10021 agatgaagaa agacaatgct gcaaaatatt cgaattgtgc tggtggagac gtcacacacc
    10081 ggcaatatgg gttctgttgc ccgtgccatg aaaacaatgg gattaaccaa tctgtggctg
    10141 gttaatccac tggtgaaacc cgactcccag gcgattgccc tggcagcagg ggccagcgat
    10201 gtgattggta atgctcacat cgtcgatacg ctcgacgaag cgttagccgg ttgtagcctg
    10261 gtggtgggca ccagcgcacg ctcccgcacg ctgccgtggc cgatgctcga cccgcgcgaa
    10321 tgcggtctga aaagcgtcgc tgaagcggca aataccccgg tggcgctggt gtttggtcgt
    10381 gagcgcgtcg gcttgaccaa tgaagagttg cagaaatgcc attatcatgt cgcgattgcg
    10441 gcaaacccgg aatacagttc gctgaacttg gcaatggcgg ttcaggttat tgcctatgaa
    10501 gtgcgtatgg cctggctggc aactcaggaa aatggcgagc aggtcgagca tgaagagacg
    10561 ccgtatccgc tggtcgatga tctggagcgt ttttatggtc atctggaaca aacgctgctg
    10621 gcaaccggtt ttatccgtga aaaccatccg gggcaggtga tgaataaatt gcgccgtctg
    10681 tttacccgtg cgcgcccgga aagccaggaa ttgaatatcc tgcgcgggat tctggcttct
    10741 atagagcagc agaataaagg taataaggcc tgagggctaa gccagataaa tgcacggacg
    10801 gtcccctcgc cccttcgggg agagggttag ggtgagggga agaggccagc gctaatgcga
    10861 ataattgccc tcaacccgcc catggtctgg aaggacaaca ggtacaaacg ccacgataaa
    10921 aaaatggcac tgaaggttaa atacccgact aaatcagtca agtaaatagt tgaccaattt
    10981 actcgggaat gtcagacttg accctgctat gcaatacccc cacttttaca ataaaaaacc
    11041 ccgggcaggg gcgagtttga ggtgaagtaa gacatgagac tgacatctaa agggcgctat
    11101 gccgtgaccg caatgcttga cgttgcgctc aactctgaag cgggcccggt accgttggct
    11161 gatatttccg aacgtcaggg aatttccctt tcttatctgg aacaactgtt ttcccgtctg
    11221 cgtaaaaatg gtctggtttc cagcgtacgt ggaccaggcg gtggttatct gttaggcaaa
    11281 gatgccagca gcatcgccgt tggcgaagtg attagcgccg ttgacgaatc tgtagatgct
    11341 acccgttgtc agggtaaagg cggttgccag ggcggcgata aatgcctgac ccacgcgctg
    11401 tggcgtgatt tgagcgaccg tctcaccggt tttctcaaca acattacttt aggcgaactg
    11461 gttaataacc aggaagtgct ggatgtgtct ggtcgtcagc atactcacga cgcgccacgc
    11521 acccgcatac aagacgcgat cgacgttaag ttacgcgctt aatttaaccg tcctgtttcg
    11581 tgttgcatct gcgttgactg cacatactca ctccggtcac ttacttatgt aagctcccgg
    11641 agattcgtaa gtttgtcaac ttgctgaaac ccgaaacagt taggttaaag ataacaagat
    11701 attagaatca ggccggggcg tatcgcgctc cgtaaacacg gtcgtacatc cagccggtag
    11761 cctggttcct tgcattgagt gatgtacgga gtttatagag caatgaaatt accgatttat
    11821 ctcgactact ccgcaaccac gccggtggac ccgcgtgttg ccgagaaaat gatgcagttt
    11881 atgacgatgg acggaacctt tggtaacccg gcctcccgtt ctcaccgttt cggctggcag
    11941 gctgaagaag cggtagatat cgcccgtaat cagattgccg atctggtcgg cgctgatccg
    12001 cgtgaaatcg tctttacctc tggtgcaacc gaatctgaca acctggcgat caaaggtgca
    12061 gccaactttt atcagaaaaa aggcaagcac atcatcacca gcaaaaccga acacaaagcg
    12121 gtactggata cctgccgtca gctggagcgc gaaggttttg aagtcaccta cctggcaccg
    12181 cagcgtaacg gcattatcga tctgaaagaa cttgaagcag cgatgcgtga tgacaccatc
    12241 cttgtttcca tcatgcacgt aaataacgaa atcggcgtgg tgcaggatat cgcggctatc
    12301 ggcgaaatgt gccgtgctcg tggcattatc tatcacgttg atgcaaccca gagcgtgggc
    12361 aaactgccta tcgacctgag ccagttgaaa gttgacctga tgtctttctc cggtcacaaa
    12421 atctatggcc cgaaaggtat cggtgcgctg tatgtacgtc gtaaaccgcg cgtacgcatc
    12481 gaagcgcaaa tgcacggcgg cggtcacgag cgcggtatgc gttccggcac tctgcctgtt
    12541 caccagatcg tcggaatggg cgaggcctat cgcatcgcaa aagaagagat ggcgaccgag
    12601 atggaacgtc tgcgcggcct gcgtaaccgt ctgtggaacg gcatcaaaga tatcgaagaa
    12661 gtttacctga acggtgacct ggaacacggt gcgccgaaca ttctcaacgt cagcttcaac
    12721 tacgttgaag gtgagtcgct gattatggcg ctgaaagacc tcgcagtttc ttcaggttcc
    12781 gcctgtacgt cagcaagcct cgaaccgtcc tacgtgctgc gcgcgctggg gctgaacgac
    12841 gagctggcac atagctctat ccgtttctct ttaggtcgtt ttactactga agaagagatc
    12901 gactacacca tcgagttagt tcgtaaatcc atcggtcgtc tgcgtgacct ttctccgctg
    12961 tgggaaatgt acaagcaggg cgtggatctg aacagcatcg aatgggctca tcattaatcg
    13021 gtatcggaat caggagaatt tataatggct tacagcgaaa aagttatcga ccattacgag
    13081 aatccgcgta acgtgggttc ctttgacaac aacgacgaga acgtcggcag cggcatggtg
    13141 ggggcaccgg cctgtggcga cgtgatgaag ttgcagatta aagtcaacga tgaaggtatc
    13201 attgaagacg cgcgttttaa aacttacggc tgcggttctg ctatcgcttc cagctccctg
    13261 gtcaccgaat gggtgaaagg gaagtctctc gacgaagcgc aggcgatcaa aaataccgat
    13321 attgctgaag aacttgaact gccgccggtg aaaattcact gttctattct ggcagaagac
    13381 gcgatcaaag ccgccattgc ggactataaa agcaaacgtg aagcaaaata agagttgagg
    13441 tttggttatg tcgattacac tgagcgacag tgcagcagcg cgagtaaata cctttctggc
    13501 taaccgcggc aaagggtttg gcctgcgtct gggcgtgaga acctccggat gttcaggtat
    13561 ggcttatgta ctggaatttg ttgacgaacc gacgccggaa gacatcgtgt ttgaagacaa
    13621 aggcgtgaaa gtcgtggtcg atggcaaaag cctgcaattt ctggacggta cgcagctgga
    13681 cttcgtaaaa gaaggcctga acgaagggtt taaattcacc aacccgaacg tcaaagatga
    13741 gtgtggttgc ggcgaaagct tccacgtttg atgcgcatac cgataacccc accgtggtcg
    13801 cctgcgcgtg gggtttgttt tacctgattc gccgttatcg cggcggatcg cagccctgag
    13861 aatgttatgg attacttcac cttctttggc ttgcctgccc gttatcaact cgatacccag
    13921 gcgctgagcc tgcgttttca ggatctacaa cgtcagtatc atcctgataa attcgccagc
    13981 ggaagccagg cggaacaact cgccgccgta cagcaatctg caaccattaa ccaggcctgg
    14041 caaacgctgc gtcatccgtt aatgcgcgcg gaatatttgc tttctttaca cggttttgat
    14101 ctcgccagcg agcagcatac tgtgcgcgac accgcgttcc tgatggaaca gttggagctg
    14161 cgcgaagagc tggacgagat cgaacaggcg aaagatgaag cgcggctgga aagctttatc
    14221 aaacgtgtga aaaagatgtt tgatacccgc catcagttga tggttgaaca gttagacaac
    14281 gaggcgtggg acgcggcggc ggataccgtg cgtaagctgc gttttctcga taaactgcgg
    14341 agcagtgccg aacaactcga agaaaaactg ctcgattttt aatttctgga agctaaacat
    14401 ggccttatta caaattagtg aacctggttt gagtgctgcg ccgcatcagc gtcgtctggc
    14461 ggctggtatt gacctgggca caaccaactc gctggtggcg acagtgcgca gcggtcaggc
    14521 cgaaacgtta gccgaccatg aaggccgtca cctgctgcca tctgttgttc actatcaaca
    14581 gcaagggcat tcggtgggtt atgacgcgcg tactaacgca gcgcttgaca ccgccaacac
    14641 cattagttct gttaaacgcc tgatggggcg ctcgctggct gatatccagc aacgctatcc
    14701 gcatctgcct tatcaattcc aggccagcga aaacggcctg ccgatgattg aaacggcggc
    14761 ggggctgctg aacccggtgc gcgtttctgc ggacatcctc aaagcactgg cggcgcgggc
    14821 aactgaagcc ctggcaggcg agctggatgg tgtagttatc accgttccgg cgtactttga
    14881 cgatgcccag cgtcagggca ccaaagacgc ggcgcgtctg gcggggttgc atgttctgcg
    14941 cttacttaac gaaccgaccg ctgcggctat cgcctacggg ctggattccg gtcaggaagg
    15001 cgtgatcgct gtttatgacc tcggtggcgg gacgtttgat atttccattc tgcgcttaag
    15061 tcgcggcgtg tttgaagtgc tggcaaccgg cggtgattcc gcgctcggcg gcgatgattt
    15121 cgaccatctg ctggcggatt acattcgcga gcaggcgggc attcctgatc gtagcgataa
    15181 ccgcgttcag cgtgaactgc tggatgccgc cattgcagcc aaaatcgcgc tgagcgatgc
    15241 ggactccgtg accgttaacg ttgcgggctg gcagggcgaa atcagccgtg aacaattcaa
    15301 tgaactgatc gcgccactgg taaaacgaac cttactggct tgtcgtcgcg cgcttaaaga
    15361 cgcgggtgta gaagctgacg aagtgctgga agtggtgatg gtgggcggtt ctactcgcgt
    15421 gccgctggtg cgtgaacggg taggcgaatt tttcggtcgt ccaccgctga cttccatcga
    15481 cccggataaa gtcgtcgcta ttggcgcggc gattcaggcg gatattctgg tgggtaacaa
    15541 gccagacagc gaaatgctgc tgcttgatgt gatcccactg tcgctgggcc tcgaaacgat
    15601 gggcggcctg gtggagaaag tgattccgcg taataccact attccggtgg cccgcgctca
    15661 ggatttcacc acctttaaag atggtcagac ggcgatgtct atccatgtaa tgcagggtga
    15721 gcgcgaactg gtgcaggact gccgctcact ggcgcgtttt gcgctgcgtg gtattccggc
    15781 gctaccggct ggtggtgcgc atattcgcgt gacgttccag gtcgatgccg acggtctttt
    15841 gagcgtgacg gcgatggaga aatccaccgg cgttgaggcg tctattcagg tcaaaccgtc
    15901 ttacggtctg accgatagcg aaatcgcttc gatgatcaaa gactcaatga gctatgccga
    15961 gcaggacgta aaagcccgaa tgctggcaga acaaaaagta gaagcggcgc gtgtgctgga
    16021 aagtctgcac ggcgcgctgg ctgctgatgc cgcgctgtta agcgccgcag aacgtcaggt
    16081 cattgacgat gctgccgctc acctgagtga agtggcgcag ggcgatgacg ttgacgccat
    16141 cgaacaagcg attaaaaacg tagacaaaca aacccaggat ttcgccgctc gccgcatgga
    16201 ccagtcggtt cgtcgtgcgc tgaaaggcca ttccgtggac gaggtttaat atgccaaaga
    16261 ttgttatttt gcctcatcag gatctctgtc ctgatggcgc tgttctggaa gctaatagcg
    16321 gtgaaaccat tctcgacgca gctctgcgta acggtatcga gattgaacac gcctgtgaaa
    16381 aatcctgtgc ttgcaccacc tgccactgca tcgttcgtga aggttttgac tcgctgccag
    16441 aaagctcaga gcaggaagac gacatgctgg acaaagcctg gggactggag cccgaaagcc
    16501 gtttaagctg ccaggcacgt gttaccgacg aagatttagt agtcgaaatc ccgcgttaca
    16561 ctatcaacca tgcgcgtgag cattaacaga ggttagtatg ggacttaagt ggaccgatag
    16621 ccgcgaaatt ggcgaagcac tgtacgatgc gtatcctgat cttgatccga aaacggttcg
    16681 attcaccgat atgcatcagt ggatttgcga tctggaagat ttcgacgacg acccgcaggc
    16741 atccaacgag aaaattctcg aagcgatttt gttagtctgg ctggacgagg ccgaataatt
    16801 tccgcgtcat gcttcacgcc gcagatgcgt tggctgcgct cgttcacccc agtcacatag
    16861 ttatctatgc tcctggggct tcactcactt gccgcctttc tgcaacgcga atcatttagc
    16921 ggaaaaatcc tggggctgcc aactggcggc ccttttacaa agaaggataa ctaaaatgac
    16981 agaagcgatg aagattaccc tctctaccca acctgccgac gcgcgctggg gagaaaaagc
    17041 aacttacagc attaataatg acggcattac cctgcatttg aacggggcag acgatctggg
    17101 gctgatccag cgtgcggcgc gcaagattga cggtctgggc atcaagcatg ttcagttaag
    17161 cggtgaaggc tgggatgcgg atcgctgctg ggcattctgg caaggttaca aagccccgaa
    17221 aggcacgcgt aaagtggagt ggccggatct ggacgatgcc cagcgccagg aactggataa
    17281 ccgcctgatg atcatcgact gggtgcgtga caccatcaac gcaccggcag aagaattggg
    17341 accatcgcaa ctggcacagc gtgctgttga tctgatcagc aacgtcgcgg gcgatcgtgt
    17401 gacttatcgg atcaccaaag gcgaagatct gcgtgagcaa ggttatatgg ggctgcacac
    17461 cgtcggacgc ggttcagaac gttctccggt attgctggcg ctggattaca acccaactgg
    17521 cgataaagaa gcgccagtgt acgcgtgcct ggtaggtaaa ggtatcactt ttgactccgg
    17581 cggctacagc atcaaacaga ctgcgtttat ggactcgatg aagtcggaca tgggcggcgc
    17641 ggcaacggtt accggggcgc tggcatttgc cattacgcgc ggactgaaca agcgcgtgaa
    17701 gctgttcctc tgctgtgcgg ataacctgat tagcggcaat gcgttcaagc tgggcgatat
    17761 catcacctat cgcaacggta aaaaagttga agtgatgaac actgatgccg aagggcgtct
    17821 ggtgcttgcc gatggtctga ttgatgccag tgcgcagaaa ccggaaatga tcattgatgc
    17881 ggcgaccctc accggggcgg cgaaaactgc gctgggtaat gattatcacg cgctgttcag
    17941 ttttgacgat gcgcttgccg gtcgtttgct ggcgagtgcc tcacaagaga acgaaccatt
    18001 ctggcgtctg ccgctggcgg aattccaccg cagccagctg ccgtctaact ttgccgaact
    18061 gaacaatacc ggaagcgcgg cgtatccggc aggcgcgagc acggcggcgg gcttcctgtc
    18121 gcactttgtt gagaactatc agcaaggctg gctgcatatc gactgctcgg cgacttaccg
    18181 taaagcgccg gttgaacagt ggtctgcggg cgctacggga cttggtgtgc gcacgatagc
    18241 taatctgtta acggcgtaaa ctaatatgcc ggatgcggcg taaacgcctt atccggccta
    18301 caaattctgc aaattcaaca aattgcgaat cccttgtagg cctgataagc gtagcgcatc
    18361 aggcatttgc gccccgaacg ttgaatgaat tgtggttctt atgtccgaaa caaaaaacga
    18421 acttgaagac ctgctggaaa aagcagcaac cgaaccggcg caccgcccgg cctttttccg
    18481 tactctactg gaatccaccg tctgggtgcc tggtacggcg gcgcagggcg aggctgtggt
    18541 tgaagatagt gcgcttgatt tacagcactg ggaaaaagaa gacggcacca gcgtcattcc
    18601 ttttttcacc tcgttagaag cgcttcaaca ggcggttgaa gacgaacagg catttgtcgt
    18661 aatgcccgtt cgcacattgt ttgagatgac ccttggcgaa acgctcttcc ttaatgccaa
    18721 actgccgacc ggtaaagaat ttatgccgcg tgaaatcagt ttgttgattg gtgaagaggg
    18781 aaatccgctg agcagccagg aaatcctgga aggcggtgaa tcgctgatat tatcggaagt
    18841 cgcagagccg ccagcacaaa tgattgattc actcaccacc ttatttaaaa ccattaagcc
    18901 ggtgaagcgt gcttttattt gttcaattaa agagaacgaa gaggcacagc ctaatttact
    18961 tattggcatt gaagccgatg gtgatatcga agaaattatt caggcgacgg gaagtgtagc
    19021 gaccgataca ttacctggcg atgaaccaat cgatatttgt caggtgaaaa aaggggaaaa
    19081 aggaattagc cactttatta ccgaacatat tgtgccattc tatgaacgtc gctggggtgg
    19141 ttttttgcgt gactttaaac aaaaccggat aatctaaaat atgccgccct aaagggcggc
    19201 attcgggctt acttgctgct ctgaaggttg ttaaccacca gtaaattgtc gccggagaga
    19261 ctccggttat tatttacttg ggaaaacagc gcaccagaaa caataagtac gatacagaag
    19321 caccgtatct tcattgtgcg gtccatgcga agaccgcatc acgacagcac cactggcgat
    19381 attgccgcga tagcaagcgc tgagacgcgc ttgctctaac ggtcgcggaa catcctttcc
    19441 ttaagccagc gcccgcttgc gagacggcgg aaagggccgc agtggataat acactaacag
    19501 aacaataata actctctttt gcttgacaaa aagagagtta ctggcgagta ttgtctccgc
    19561 tgagacgcgc ttgcattact acggtaatac aacctgctgg aaaggccgcg aaccagacca
    19621 gcaataaaaa accgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg
    19681 gctcaaccgg taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca
    19741 cgtttggcac atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc
    19801 cgcagctgac gataattggt ttgtcgtagc tgacgccgcg accaaaaaat atcgcatcca
    19861 gttcatcggt cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acattcagtg
    19921 ctccgggaat atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac
    19981 gtgcagccgg gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataatacat
    20041 cggttacttt caccacggct tcaggattaa acgcggcgtt aaactctcct tccggcagct
    20101 ctactgcacc ttcttctaac agcagatcat cgcgctgcca gcctgcaagt ccacccccca
    20161 gaatcgacac tttctctaca ccaaaggtgc gcagcatcca ccatgctcgt ggggctgaga
    20221 aaagattacc ttcgtcatag acaatcaggt gcttatcctg gttaacgcct aattcacgca
    20281 tcgccacggc gaacgtttcc gggcgcggca gcatgtgcgg aagcggggaa gtgtgatcag
    20341 aaagcgcttc gatatcaaaa aacactgcgc cgggaatatg tccattcaga tactcctgag
    20401 caacgttacg atcttcctgt ccaggcgacg ccatgcgggc atcgataatc tgaatttccg
    20461 ggtcatcaat atgttcggcg agccagtcgg ctcctacaaa ccatgtcgtg gacataggca
    20521 tctccatttt tgccggggtt aggctaaatt gtcggatgtt tagtgaaaag cgacaagcga
    20581 aagaggctga tttggctgtc tgagccacaa ttctcactaa aattcggatt gtatgggcgt
    20641 gatactgaat
//
LOCUS       DAEEBB010000071        20399 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEEBB010000071 DAEEBB010000000
VERSION     DAEEBB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.071"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(130..1779)
                     /gene="actP"
                     /locus_tag="KLF07_002413"
     CDS             complement(130..1779)
                     /gene="actP"
                     /locus_tag="KLF07_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBD9832415.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1776..2089)
                     /locus_tag="KLF07_002414"
                     /pseudo
     CDS             complement(1776..2089)
                     /locus_tag="KLF07_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2289..4247)
                     /gene="acs"
                     /locus_tag="KLF07_002415"
     CDS             complement(2289..4247)
                     /gene="acs"
                     /locus_tag="KLF07_002415"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBD9832416.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4641..6077
                     /gene="nrfA"
                     /locus_tag="KLF07_002416"
     CDS             4641..6077
                     /gene="nrfA"
                     /locus_tag="KLF07_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBD9832417.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6122..6688
                     /gene="nrfB"
                     /locus_tag="KLF07_002417"
     CDS             6122..6688
                     /gene="nrfB"
                     /locus_tag="KLF07_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBD9832418.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6685..7356
                     /gene="nrfC"
                     /locus_tag="KLF07_002418"
     CDS             6685..7356
                     /gene="nrfC"
                     /locus_tag="KLF07_002418"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBD9832419.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7353..8309
                     /gene="nrfD"
                     /locus_tag="KLF07_002419"
     CDS             7353..8309
                     /gene="nrfD"
                     /locus_tag="KLF07_002419"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBD9832420.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8329..10047
                     /locus_tag="KLF07_002420"
     CDS             8329..10047
                     /locus_tag="KLF07_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBD9832421.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10040..10423
                     /gene="nrfF"
                     /locus_tag="KLF07_002421"
     CDS             10040..10423
                     /gene="nrfF"
                     /locus_tag="KLF07_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBD9832422.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10420..11016
                     /gene="nrfG"
                     /locus_tag="KLF07_002422"
     CDS             10420..11016
                     /gene="nrfG"
                     /locus_tag="KLF07_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBD9832423.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11358..12671
                     /gene="gltP"
                     /locus_tag="KLF07_002423"
     CDS             11358..12671
                     /gene="gltP"
                     /locus_tag="KLF07_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBD9832424.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12750..13439)
                     /locus_tag="KLF07_002424"
     CDS             complement(12750..13439)
                     /locus_tag="KLF07_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD9832425.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="KLF07_002425"
     CDS             complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="KLF07_002425"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15261..15263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBD9832426.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="KLF07_002426"
     CDS             complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="KLF07_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBD9832427.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="KLF07_002427"
     CDS             complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="KLF07_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBD9832428.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="KLF07_002428"
     CDS             complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="KLF07_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBD9832429.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5435 c         5601 g         4808 t
ORIGIN      
        1 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
       61 ttgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc gaccggggaa
      121 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      181 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      241 gaaccagatg ccgaggaatg ccacggtgat agagaacagc gccgggtatt catacgggaa
      301 gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga tcatcagtac
      361 tactgcggta atcagcccca gccagccacc catcatcgcg ccacgcgtgg tcagtttcga
      421 ccagtacata gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgccag
      481 ccccaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      541 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      601 tttgaagacg ttagcgtaca agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc
      661 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt
      721 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      781 cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga agccgataat
      841 aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc tcttacgtgc
      901 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
      961 aaacatcagt cccagaccca gagagagtgc ggagatcgga tctttcacca gcccgcccgg
     1021 cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca gattgttgaa
     1081 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1141 cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca ggacgtacat
     1201 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1261 gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca ccaccagaga
     1321 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg ccacatcggc
     1381 aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca
     1441 gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa acaccagcgc
     1501 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1561 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgagttttga
     1621 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1681 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1741 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1801 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1861 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1921 ggattcagcg gcgtgccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat
     1981 aaactgccag cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact
     2041 ccctgaaatg cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc
     2101 cagaggtaat gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg
     2161 ggtaaccctt gccgaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaatgc
     2221 ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc gcatccggca
     2281 attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt ctcgactacg
     2341 ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg
     2401 cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc ggtccagtgc
     2461 agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg gacttctgcg
     2521 tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga
     2581 cctttaatat tgtgcggaat acctactacg gcggcttcgg caatcttcgg atgcgccacc
     2641 agcgccgact caatctctgc cgtccccaga cggtgaccgg agacgttcag cacgtcgtcc
     2701 acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa
     2761 tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac gttcgtgatc gccaaacagc
     2821 gtacgcgcct gacccggcca ggagtcggtg atcaccaggc tgccttcggt agcgccttcc
     2881 agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc
     2941 gaaccggctt tcagctcggt agcgccaggc agcggcgtga tcatgaaacc gccagtttcg
     3001 gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt ccagtaccac
     3061 tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag cgacgaacgg
     3121 tcggtaccct cgatcgcttt atcgccttcc gccatcagcg cgcggatcgc cgtgggtgcg
     3181 gtatagagaa tattgacctg atgcttgtcc accacctgcg ccatacgggc aggcgtcggc
     3241 cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc aggccagcgg gccgtacagc
     3301 aagtaactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat atcacccgga
     3361 tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc gccggtagta
     3421 tgcagcacac cttttggctt accggtagaa ccggaggtgt agagaataaa cagcggatct
     3481 tcggcgttca tcttttccgc ctggtgctga tcgctcgctt gctcaaccag gtcgtgccac
     3541 cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag taccaccaca
     3601 tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat caacgttttt cttcagcgga
     3661 atactgcgcc cggcacgcac accttcgtcg gaagtgatca ccagtcgtga gttggaatca
     3721 ataatgcgcc cggcaacggc ttccggcgag aagccgccga aaatcaccga atgcaccgcg
     3781 ccaatgcggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg catataaatc
     3841 gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa gcggcagacg
     3901 tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc gccttcccag
     3961 atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt tgccgccaga
     4021 ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa ggaggtgttt
     4081 ttcaccttct ggtaaggttt gatccagtca agaatttttc cctgttcgcc ccagaaggta
     4141 tcaggtacgt taatagattg ttgatacatc gcctcgtact gctgagggtt tatcaggcaa
     4201 cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt ggctcatgct tttgttctcc
     4261 ttgtagggtg ttaataatat gtggcataag cgttaaatgt aggggtattg gcagttttgt
     4321 ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg gcagggtaat
     4381 ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt tatgcattgc
     4441 tgttgaaaaa gaggaagata ctaactaact ctaaagtggt attttacatg cacttacaat
     4501 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat
     4561 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg taacaatggc
     4621 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4681 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4741 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4801 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4861 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     4921 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     4981 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5041 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5101 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5161 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5221 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5281 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5341 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5401 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5461 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5521 aaggcaaact ctataccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5581 gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5641 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5701 cgaaagcagc gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata
     5761 tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg
     5821 caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     5881 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     5941 tctcaaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6001 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg
     6061 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6121 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag
     6181 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6241 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6301 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6361 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6421 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6481 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6541 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6601 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6661 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6721 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6781 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6841 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6901 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     6961 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7021 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7081 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7141 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgtgt tgaggcttgc
     7201 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7261 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7321 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7381 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7441 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7501 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7561 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7621 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7681 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7741 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7801 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7861 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     7921 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     7981 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8041 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8101 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8161 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8221 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8281 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8341 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8401 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8461 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8521 ctcatcagcg atttctcggt catttatgtc gcgcaacata gccatagcct gctgtcgtgg
     8581 gaattcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8641 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8701 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8761 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8821 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8881 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     8941 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9001 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9061 gatccggtgg aaaatgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9121 ttatccctga cacgccagcg ggggattttt tgccactggt cgctgttgct ggcgatagtt
     9181 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9241 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9301 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9361 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9421 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct gggctgggga
     9481 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9541 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg
     9601 gtcgctcatg cgggcgtgct gttatttgcc gcagggatcg tggtttccag cgtcagccgt
     9661 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9721 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9781 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9841 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9901 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta tgtacaaagc
     9961 ggtgtgcgct ggatctgggg tggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10021 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10081 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10141 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10201 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10261 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10321 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10381 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10441 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10501 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10561 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10621 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10681 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10741 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10801 cagacttgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10861 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10921 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    10981 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11041 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat
    11101 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11161 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11221 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11281 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11341 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11401 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11461 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11521 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11581 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11641 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11701 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11761 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11821 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11881 cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    11941 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12001 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12061 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc
    12121 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12181 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12241 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12301 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12361 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12421 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12481 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12541 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12601 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12661 cggatcaata attgaagatt gccggggata tccacccggc aatgtgtgaa tgcctgatgc
    12721 gacgcttgcc gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca
    12781 cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg
    12841 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    12901 taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    12961 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    13021 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatagcctt tggataatcc
    13081 ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    13141 cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    13201 ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct
    13261 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    13321 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    13381 gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt
    13441 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    13501 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    13561 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    13621 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    13681 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    13741 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    13801 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    13861 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    13921 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    13981 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    14041 ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac
    14101 tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga
    14161 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    14221 gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct
    14281 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    14341 gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    14401 acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    14461 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    14521 ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg
    14581 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    14641 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    14701 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    14761 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    14821 tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    14881 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    14941 gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc
    15001 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    15061 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    15121 tgattcgcca cgattgggtg ggaatcagcc gggttgtacc cgaaaacgaa cactaaatcg
    15181 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    15241 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    15301 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    15361 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggta
    15421 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    15481 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca
    15541 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    15601 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    15661 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    15721 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    15781 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    15841 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    15901 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    15961 tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct
    16021 gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg
    16081 ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc
    16141 cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca
    16201 tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    16261 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    16321 ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    16381 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    16441 ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta
    16501 atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag
    16561 attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    16621 tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct
    16681 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    16741 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    16801 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc
    16861 gatgcacacc ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat
    16921 agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt
    16981 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    17041 catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg
    17101 tgtgtgaacc acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt
    17161 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    17221 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt gcgtaccaag gcacagccgg
    17281 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga
    17341 tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    17401 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    17461 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    17521 cagaccggca gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    17581 tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg
    17641 gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag
    17701 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    17761 accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg
    17821 cattacttta cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    17881 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    17941 cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt
    18001 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    18061 ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt
    18121 gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc
    18181 cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat
    18241 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta
    18301 acagatcaaa caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa
    18361 agcatcggcg accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat
    18421 ctggcccagt tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac
    18481 cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg
    18541 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    18601 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    18661 gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac
    18721 attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    18781 gctgtctgtc aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg
    18841 cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata
    18901 gaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat
    18961 ggcggggaag gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag
    19021 ccgatgggtg cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg
    19081 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19141 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19201 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag
    19261 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19321 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt
    19381 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19441 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19501 ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    19561 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19621 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19681 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    19741 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    19801 atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19861 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19921 agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca
    19981 aaaccctctt tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg
    20041 gcacgggctt tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg
    20101 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    20161 agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc
    20221 tggttgtcgg tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg
    20281 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20341 aaaacaaggg caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagg
//
LOCUS       DAEEBB010000072        20298 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEEBB010000072 DAEEBB010000000
VERSION     DAEEBB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.072"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..69
                     /locus_tag="KLF07_002429"
     CDS             <1..69
                     /locus_tag="KLF07_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBD9832430.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(70..174)
                     /locus_tag="KLF07_002430"
     CDS             complement(70..174)
                     /locus_tag="KLF07_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832431.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            344..790
                     /locus_tag="KLF07_002431"
     CDS             344..790
                     /locus_tag="KLF07_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBD9832432.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(747..1568)
                     /gene="nimR"
                     /locus_tag="KLF07_002432"
     CDS             complement(747..1568)
                     /gene="nimR"
                     /locus_tag="KLF07_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBD9832433.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1665..2846
                     /gene="nimT"
                     /locus_tag="KLF07_002433"
     CDS             1665..2846
                     /gene="nimT"
                     /locus_tag="KLF07_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBD9832434.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2901..3248
                     /locus_tag="KLF07_002434"
     CDS             2901..3248
                     /locus_tag="KLF07_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBD9832435.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="KLF07_002435"
     CDS             complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="KLF07_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBD9832436.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3707..4732
                     /gene="dgcP"
                     /locus_tag="KLF07_002436"
     CDS             3707..4732
                     /gene="dgcP"
                     /locus_tag="KLF07_002436"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBD9832437.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4765..4863
                     /locus_tag="KLF07_002437"
     CDS             4765..4863
                     /locus_tag="KLF07_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBD9832438.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4866..4940
                     /gene="yoaJ"
                     /locus_tag="KLF07_002438"
     CDS             4866..4940
                     /gene="yoaJ"
                     /locus_tag="KLF07_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBD9832439.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4999..5247)
                     /locus_tag="KLF07_002439"
     CDS             complement(4999..5247)
                     /locus_tag="KLF07_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBD9832440.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5395..5577)
                     /locus_tag="KLF07_002440"
     CDS             complement(5395..5577)
                     /locus_tag="KLF07_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBD9832441.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="KLF07_002441"
     CDS             complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="KLF07_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBD9832442.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="KLF07_002442"
     CDS             complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="KLF07_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBD9832443.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="KLF07_002443"
     CDS             complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="KLF07_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBD9832444.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7904..8989
                     /gene="dmlA"
                     /locus_tag="KLF07_002444"
     CDS             7904..8989
                     /gene="dmlA"
                     /locus_tag="KLF07_002444"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBD9832445.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9240..10850
                     /gene="yeaV"
                     /locus_tag="KLF07_002445"
     CDS             9240..10850
                     /gene="yeaV"
                     /locus_tag="KLF07_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBD9832446.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10882..12006
                     /gene="yeaW"
                     /locus_tag="KLF07_002446"
     CDS             10882..12006
                     /gene="yeaW"
                     /locus_tag="KLF07_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBD9832447.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12062..13027
                     /gene="yeaX"
                     /locus_tag="KLF07_002447"
     CDS             12062..13027
                     /gene="yeaX"
                     /locus_tag="KLF07_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBD9832448.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="KLF07_002448"
     CDS             complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="KLF07_002448"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBD9832449.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="KLF07_002449"
     CDS             complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="KLF07_002449"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBD9832450.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="KLF07_002450"
     CDS             complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="KLF07_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBD9832451.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="KLF07_002451"
     CDS             complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="KLF07_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBD9832452.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17542..19452)
                     /locus_tag="KLF07_002452"
     CDS             complement(17542..19452)
                     /locus_tag="KLF07_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBD9832453.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19584..19928
                     /locus_tag="KLF07_002453"
     CDS             19584..19928
                     /locus_tag="KLF07_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD9832454.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4934 a         5248 c         4949 g         5167 t
ORIGIN      
        1 attttgaaaa ccacggatta tttgcgcatt gcgcaaccag aactgctcaa tttccgccgc
       61 actgcgtaac tagcctgtcc gttcaataag cagaacggac aaaaccagca caacagcaat
      121 ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg
      181 cccccgggag atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac
      241 aattcgcctg attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaaa
      301 cgtaaagtaa gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt
      361 gctgatcctg ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt
      421 ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat
      481 tgaaaaacag gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat
      541 cgccagcggg acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct
      601 ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt gggcgcggcg tgaccttaat
      661 gggcagccag ccgcaactgg tcgccgggct gctggtaggc actgttttag gcgtggcgct
      721 gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg
      781 gaaacagtag ttaatcccgc aatatatcgg cctggcgttt gccccaggcc tttcttaaac
      841 atagtaataa aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta
      901 tcgcctttca ctaatccctg aagtgccata atcagctgta actgctgacg ccactggcga
      961 aaactcaacc ccgtctcttt tactatcagt cgcgcaagat tgcgttcact catcgcaaaa
     1021 aagccagccc attgccctaa tgacccccac tcgacgggcc ctttcgccat catctccacc
     1081 atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga
     1141 gggagttcat caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc
     1201 gtcgtcctgt ttgccagggt taaaatcaat tcccggcata agggcgatat tttcaatgtg
     1261 caacatgttg tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg
     1321 ttagctgtca cttgattgct atgttccacc ccgcccggta tccaaaccgc atattgcggc
     1381 ggcaccatcc ataaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc
     1441 agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata
     1501 caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg
     1561 tgcatcattt tgtccgaatt tagcgataat ttgtcatttt agcttgattc aacataacaa
     1621 taaaaacggt aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt
     1681 cattaagcgg caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat
     1741 tacgcgtcac ctttatcggc gcagcaccgt tactggatac gattcgttcc gcttactcgc
     1801 tgacgacagc gcaaaccggc ttattgacca ccctgccatt attggccttt gcgctaatct
     1861 cacctttggc caccccggta gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt
     1921 tacttttgat ctgtgctggt atcgcaattc gctctctccc ttcgccttac ttattatttg
     1981 gcggtacagc ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa
     2041 ttaaacgcga tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg
     2101 gagctgcagc ggcactggga tcggctatga tagtgccgct ggctttgaac ggttttggct
     2161 ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc
     2221 cacagtggcg aagccaacaa catgcaaatt tgagtacctc gcgcgcctta catactcggg
     2281 gtatctggcg ttcgccgctt gcctggcagg taacattgtt tcttgggatc aactcactga
     2341 tctattacgt gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag
     2401 cacaggcggg ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga
     2461 tccctctttt cttacatcat gtgaaagatc agcgtggtat tgcagcgttc gttgccttga
     2521 tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc gcacgcgatc acctggactc
     2581 tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc
     2641 gggcaagttc tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt
     2701 tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtgactggt
     2761 ccgtaccact gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg
     2821 ccgggagaga caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcatgcg
     2881 tgaaagggag aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac
     2941 agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg
     3001 atttagccct tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct
     3061 ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca atatcttgca gaactggcgc
     3121 aacacgagca agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc
     3181 tctacgcagc aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta
     3241 gcttgtgatt ttagtacagc atccggcggt tatttttcac cagccggatg gtcccgtcgc
     3301 cacaatgccc attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct
     3361 tgcgctgtcg acactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga
     3421 tttgccatac caatctgcgg cggtttaggt acttctggct gagaagaggt tgaacaacca
     3481 gccaggacca gcaagcaagg caaagcaaaa ctgataattt tcatttattg atctcactca
     3541 tttatctacg cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat
     3601 tcactcaaaa catattacgt cttgtttcat ctttgttggt gatgttttat catgcctgca
     3661 aagattaaat aatcagcatt tacccgccgt atcctggagt tgtttcgtgt cagatcagat
     3721 tatcgcccgc gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca
     3781 gcttctggag atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga
     3841 tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc
     3901 ggagaatttt accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg
     3961 ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg
     4021 catctccaca tttctgagca caccaattca cttaccggat ggatcattct atggcacgct
     4081 ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt
     4141 attcgccgga ctgattgcac aatatattca aaaagaggca ctggttgaac agttgcgcga
     4201 agccaatgct gcgctgattg cgcaatcgta taccgactcg ttaaccgggt tgccgaatcc
     4261 gcgggcgatt tttgaaaatc tgacgacgct gttttctctc gcccggcatc ttaaccataa
     4321 gataatgatc gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca
     4381 taatagtggc gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa
     4441 tggcgaagtt attggtcgtc tcggcggtga tgagtttttg gttgtttcac taaacaacga
     4501 gaatgcggat atttcgtcgc tgcgagaacg cattcagcag caaatacgtg gagaatatca
     4561 cttaggtgat gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc
     4621 tgaaacaacc gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa
     4681 aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg ccacattgct gaggcgtatt
     4741 cacatccttt tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttt
     4801 attacagcgg tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggggca
     4861 taaagatgaa aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca
     4921 ctgagctggg atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc
     4981 ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc
     5041 accaataacg gcaaccacga agctgccaaa attgaagcca tcgactttac caaagccaaa
     5101 cagcgtgctg atccatccgc cgactacggc accgactatc cccagcagga tagtcataaa
     5161 gaatccacca ccatctttac ctggcatgat ccacttcgcc agaataccgg caataagccc
     5221 aaaaataatc catgacagaa tgcccattgt ttcctcactt atctgttttg cattagcggg
     5281 ttagtcgctg ataaaaagca tagcacaaca tcgggagggc aagatttgtg acgagcatca
     5341 cggaggattt ttttgcgatg gcgcagaaat tgcgccatca acgatcagtg ataattacca
     5401 accacaaaca tcatgttcgt tttccgtgtc ataagaacgc acggtattca ccagatcttt
     5461 tatcacttca gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc
     5521 gacagaaacg ccattactgt tattggtgac ggtcacggta tacgttgctt tgcccatgat
     5581 tcatttcccg ttatgaatga ctttccgttg ttgcgcacct tccatcagga cttcaggagc
     5641 cacgaagaag tcaatgttga aataagtatc gtcagtcatg gcttcaatgt tgtgccactt
     5701 ttctggaggg aacacctcaa actgccccgc ttcgataagg atcacctgat caggctctgc
     5761 actgtgttca tcagcgtagc cgagatattt gaccgcccca tgcataacgg aaaggcgtgg
     5821 gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg aatattccgg caggcgcagt
     5881 ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga tttgcagcat
     5941 ccttttccct ccttcggtga atgcgctgaa aacggtttat tccagccgtt tcagggtacg
     6001 cctgataatt tgcattttaa ataccattta ttggttactt tttagcacca tatcagagaa
     6061 aaatcaggga ggattacaag tgggaagccc gcgacaacgc gggctgaatg tatcaggatt
     6121 gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt gagttgccaa
     6181 ctttagccag tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca
     6241 ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa
     6301 ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa
     6361 tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg
     6421 gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc
     6481 ccagatagag caaataaaac gcaccaagat aacgtacaat gttgaataat atcggggtgg
     6541 tcttaattaa tgtcgccact ccagcccatg ccagaaacat caatactgca tcgccaataa
     6601 atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta
     6661 caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc
     6721 agtaattcag aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat
     6781 gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg
     6841 ttttaacccg atatcaaccc gataattgaa taattaatca ggcatgctcc agtgaaaaat
     6901 tcgggtagtg ctcggcaaaa tactggcgta aaaattctat cgttatccgc actttcgctg
     6961 acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta
     7021 gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag gagcgcagag
     7081 caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg
     7141 ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag
     7201 gatgatcgcg ctctttaatg accaggcacg gcaatgcgga aagatcggtt aaatgtttcg
     7261 gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttagtcgcca
     7321 gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcaacac
     7381 cttcattgac caaatcgacc aaccggtctt ccacatcaaa acgtaattcc agttgcggat
     7441 acgccttagc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc
     7501 tgataatacg cagcatcccc tgcggcacct gacgcacgtc agaaagttcg tccatcatct
     7561 gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa
     7621 tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg
     7681 cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac
     7741 cagcccggcg agccaccagc ataaagacgc gcaaatcgtt cagcagcggt aaattattca
     7801 tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg
     7861 atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg
     7921 tattgctgcg atcccgggag atgggattgg caaagaagtc cttcctgaag ggattcgcgt
     7981 attacaggct gccgctgagc gctggggctt cgacttgagt tttgagcaaa tggagtgggc
     8041 gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact
     8101 tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggataccg ttccggacca
     8161 tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct
     8221 gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga
     8281 catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag
     8341 agtgaatgaa ggtacagagc atgaagtcgc cattcaggaa tcggtattta cgcgtcgtgg
     8401 tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct
     8461 aacttctgcc actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt
     8521 ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct
     8581 ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttgtt
     8641 tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc
     8701 cgccaacctg aatccggaac gcactttccc gtcgctcttc gagcctgtcc acggttccgc
     8761 gccggatatc tacgggaaaa atattgctaa ccctatcgcc acaatttggg ccggtgcgat
     8821 gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct
     8881 ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca ccggatatga aaggcagtgc
     8941 caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag
     9001 tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt taataacctg
     9061 cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg
     9121 tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc
     9181 tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca
     9241 tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc
     9301 ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacacaa
     9361 tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa
     9421 tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa
     9481 aaccggaata cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt
     9541 ctacgcttta ttgggggggt gctgaatggg cctattatta tcaaacgcct ggattaaata
     9601 tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg
     9661 gcatcagcac ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg
     9721 tgcgtaaaaa caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc
     9781 cgcaaggccc atggggaaaa ctggttgatt tgatgtttct gatcgccact gtcggcgcac
     9841 tgactatttc ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg
     9901 gtttacccga taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt
     9961 gcctaagctc gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct
    10021 ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg tccaaccgaa tttattacca
    10081 acagcatcat caatgccatc ggcctgacca cgcaaaactt cctgcaaatg agcttattca
    10141 ccgatccgct tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt
    10201 ggatctcata cccccctggc gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga
    10261 ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg
    10321 gcgtaatgga aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg
    10381 tgctggaaac actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg
    10441 gtaaattgtt cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg
    10501 atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa tctccaggaa ggtgacgatc
    10561 ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct
    10621 tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct
    10681 tcctcgtcat tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattatg
    10741 ccgacattcc ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg
    10801 aaaaaacacc agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat
    10861 cctaacgata aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat
    10921 ttttgcgcta acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac
    10981 gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc
    11041 agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg
    11101 ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt
    11161 catcagttgt tgagcggtga aggaaaagca aaaaatgtga ttacctgccc atatcacgca
    11221 tgggcattca aactcgatgg caatctggcc catgcacgta actgcgaaaa cgtcgccaat
    11281 ttcgatagcg acaaagcgca actggttccg gtgcgtctgg aggaatatgc cggattcgtc
    11341 ttcatcaaca tggaccccaa cgccaccagc gtagaagatc agttacccgg tctgggcgcg
    11401 aaagtgctgg aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc
    11461 cgcacgcctg ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt
    11521 ccggcgcatc caggtttctc cgactccgta caggttgatc gctactggca caccatgcac
    11581 ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa
    11641 gagggtacgg atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc
    11701 accccgatca aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc
    11761 ctgcaaaact acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg
    11821 attgagtggt atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag
    11881 aaagggctga aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt
    11941 agtggcattt ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag
    12001 gactaatgac atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc
    12061 aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca
    12121 ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg
    12181 aagtcacatc attgtgcaga tgagcgatgg tgataaccag tacagcaatg cgtattcact
    12241 actgagttcg ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc
    12301 gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc
    12361 aacgcctaat aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg
    12421 cggtattggt atcacccctt tcctgtcgca catggcagag ttgcaacaca gcgacgtcga
    12481 ctggcagcta cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt
    12541 ccagcatccg caggctgaga aagtccattt gcatcattcg tcaaccggaa cacgactgga
    12601 attagcgcga ttattggcgg atatcgaacc tggcacacac gtttatacct gtggccccga
    12661 ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgccg ccgatacgct
    12721 gcactttgag caatttgcta tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc
    12781 ccgttccgga aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa
    12841 taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac
    12901 agcaatactg gaaggtgaag ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc
    12961 cagccagcaa agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga
    13021 tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt
    13081 ttactgcgga tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca
    13141 gccggaaatc agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag
    13201 ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac
    13261 gtcagtaatc agcgacttaa tcgctttaaa cgctttacga taacccggca tgtccatcag
    13321 gttaagcatc ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc
    13381 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
    13441 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
    13501 gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc cagtcggcta acagttgcag
    13561 acaggccagt tggcgtgtgc gtaattgcca ggcattggtg atatcacgcc aggcatcttc
    13621 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
    13681 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
    13741 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
    13801 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
    13861 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca ggggttgtgg
    13921 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
    13981 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
    14041 atcaattagc gccagatgct caccatcgaa aagttgaatc aaccccagct gcgggtaata
    14101 agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc ggaaagacac ggacggcttc
    14161 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgtattctct
    14221 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacgctca
    14281 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
    14341 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt
    14401 gagctgacgg cagcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac
    14461 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
    14521 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc
    14581 ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat
    14641 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatcgg tagcatcggg
    14701 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
    14761 cggtggtact tcattatcgt cgtcatccac cagtttggct tccgtcgacg gcaccggcaa
    14821 accgatgcta ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca
    14881 ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc
    14941 caccacttgc tgcactggca tcccaccgcc tgcggaaaga tgcagactgg agaaatccag
    15001 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc cagtgatagc
    15061 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat
    15121 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
    15181 gtgatacagc ggcagtgccg ttactaccag ttctttacct ggatgcagca acggcccata
    15241 ggtggcgttg acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
    15301 cgccacacct gtggtgccgc cggtgtattg caggaaggct aaatcttccg gcaccagttc
    15361 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
    15421 tggcagatgg tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt
    15481 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
    15541 tttatcaacc actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc
    15601 gccgctatcg ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac
    15661 gacgatcatc ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg
    15721 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
    15781 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa
    15841 cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac atatctatca gagattgata
    15901 acggtcaggg ttgatctccg tcggaacatc cgcgggataa cggttaagcc aaaccttctt
    15961 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaagtg ataaacatgt
    16021 tgttaacata acattaactc atcttaccag cttgataatt agacaacgaa aaggttgcga
    16081 agcgcgtcac tatttatttt tatagttgcc gtatgaacgc agaaacagcg gaccagccgc
    16141 tgtttctttt tctttaaaaa caacttgtta ctcggttaca attgtttcaa ctcttgcagg
    16201 gccaggatta taccaacccc atccgccgta accatagggc cagccgcgtc caccataaaa
    16261 ccatggatca attggctgcg gcggcataat cacctgttga gtgatatgcc agcgtttgta
    16321 acctgttacc tgcataacca taaatttata tggtgcatta tttactttgc catccactgt
    16381 gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga aaatcgaccg gatcaagaaa
    16441 accgttcaca tcggcataaa tgcgcccgcg tgaaggttct cccaaggttg gtcttgctcc
    16501 gctgtccagc ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccac
    16561 aaccactttg ccgccaaagc gcgcctcctg accaacatat aaatctggcg cattcatcac
    16621 gcgtaccaga tcttgctgtg gtgttggact gctgccttta atggcgtccg gcacagtgac
    16681 acaaccgctc agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg
    16741 aaccgccatg atgcgactcc tttttttctc agggcatact cttaagattc attctttgcc
    16801 cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc
    16861 caccgtttta ccctcggcaa acatctgaca cgcaatcggc agcatatctt ccgccgctgg
    16921 cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttacggagat ccggccaggc
    16981 ttgccagccc gtccctaccg ttacccattc gccggaaagc tgttgcattc gttcatgaac
    17041 gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc cagataccgt tttcatcacg
    17101 ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt
    17161 tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt agcgtagaga caccaatcat
    17221 cggtaactcc gcgccaagcg ccagcccttg cgcaatacca ataccaatgc gcacgccagt
    17281 aaagctaccg gggccgcgcc cgtaagccag agcgtttatg tcagtcaggg aagttccgct
    17341 ggtggtcagg atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag
    17401 ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg acagagcaga cctctgtcgc
    17461 ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat
    17521 cataccatac tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg
    17581 ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg
    17641 acgcatcacc agccgattgt cacaaattac cagcacgccg cgatcgtcgg cgtcgcgaat
    17701 cagtcgccct actccctgct tgagagtaat gaccgcatcc ggcagctgca cttcatcgaa
    17761 cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct tttaacagta gatcatccgg
    17821 cgaggtaaac ggcaatttgt cgataattac caatgacaat gtatcgccac gcacgtccac
    17881 cccttcccag aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg
    17941 caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg
    18001 gaactgctcg gccagatcgc gcatcatggc atgcgaggta caaagcataa aacaacgacc
    18061 gttgttagct tcgatgatcg gtcgcagcat tgccgccagt tgccgtgcac aacccggttg
    18121 attggtttgc ggcaggttgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg
    18181 gcttggcaat agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag
    18241 atcgtcgttc accgacagcg ttgctgaggt gaagatccag ctaccgggct tttgcgccat
    18301 taactctttg aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga
    18361 agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg
    18421 tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga
    18481 cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg
    18541 aatttgcgga ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg
    18601 cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg cacttttgta actgctgggt
    18661 gtcttttaat tcggtgcggt aggcgatggt gatgtctttt gccaggtcga gcagttgtcg
    18721 actggagagt gactgaccaa aatactggct ggcaatgtcc ggtagctggt gggcttcgtc
    18781 gaagatcatg acgtccgctt cagggatcaa ctcgccaaat ccactctctt taaccaccat
    18841 atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg
    18901 tgctttgacc acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt
    18961 ggtgctggtg accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat
    19021 atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg
    19081 taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgttcgaggc agaggtagtt
    19141 tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg
    19201 caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt
    19261 ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc
    19321 ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg
    19381 ctgtggttct cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa
    19441 atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct
    19501 ttcgccagtt gaagtggtga cggcaacctc acattgtggc agtctttgca gcacgaaaat
    19561 ggaagtataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt
    19621 ctgatgtagt aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg
    19681 ccgatgcctt tgagcaaacc gccaacacgc tggcacagat tgacgccgtg ctggaaaaac
    19741 agggcagcaa taaatcgagc attctggatg ccaccatttt cctggccgat aaaaacgact
    19801 tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccagtgcgct
    19861 gcacggtaca agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg
    19921 cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg
    19981 gttttacata aatcgaccag agaataagat attactgttg atgcttctgc taattcatta
    20041 tttatattta taatttcaat tttatctata aaactaacta tataaataaa cataaccatg
    20101 ccgttgtgtt cgccctttga ttagcaactc tggttttctt tcttagctga acaatctggt
    20161 tatagcgtgc ggactgaccg ttaattttct ccttcttact ggataacaca ccatgagctg
    20221 ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg ccatatcgtt ataatccctt
    20281 tgatattaga tgcaaatt
//
LOCUS       DAEEBB010000073        20230 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEEBB010000073 DAEEBB010000000
VERSION     DAEEBB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.073"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            689..1459
                     /locus_tag="KLF07_002454"
     CDS             689..1459
                     /locus_tag="KLF07_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBD9832455.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="KLF07_002455"
     CDS             1450..1923
                     /locus_tag="KLF07_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBD9832456.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="KLF07_002456"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="KLF07_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBD9832457.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="KLF07_002457"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="KLF07_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBD9832458.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="KLF07_002458"
     CDS             3359..3853
                     /locus_tag="KLF07_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBD9832459.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="KLF07_002459"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="KLF07_002459"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBD9832460.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="KLF07_002460"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="KLF07_002460"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBD9832461.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="KLF07_002461"
     CDS             7698..8612
                     /locus_tag="KLF07_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD9832462.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10178
                     /locus_tag="KLF07_002462"
                     /pseudo
     CDS             8827..10178
                     /locus_tag="KLF07_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="KLF07_002463"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="KLF07_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="KLF07_002464"
     CDS             complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="KLF07_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBD9832463.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12719..14008
                     /locus_tag="KLF07_002465"
     CDS             12719..14008
                     /locus_tag="KLF07_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBD9832464.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14005..15471
                     /gene="hpaE"
                     /locus_tag="KLF07_002466"
     CDS             14005..15471
                     /gene="hpaE"
                     /locus_tag="KLF07_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBD9832465.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15473..16324
                     /gene="hpaD"
                     /locus_tag="KLF07_002467"
     CDS             15473..16324
                     /gene="hpaD"
                     /locus_tag="KLF07_002467"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBD9832466.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16334..16714
                     /locus_tag="KLF07_002468"
     CDS             16334..16714
                     /locus_tag="KLF07_002468"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBD9832467.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16825..17628
                     /gene="hpaH"
                     /locus_tag="KLF07_002469"
     CDS             16825..17628
                     /gene="hpaH"
                     /locus_tag="KLF07_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBD9832468.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17639..18427
                     /gene="hpaI"
                     /locus_tag="KLF07_002470"
     CDS             17639..18427
                     /gene="hpaI"
                     /locus_tag="KLF07_002470"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBD9832469.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18602..19978
                     /gene="hpaX"
                     /locus_tag="KLF07_002471"
     CDS             18602..19978
                     /gene="hpaX"
                     /locus_tag="KLF07_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBD9832470.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19988..>20230
                     /locus_tag="KLF07_002472"
     CDS             19988..>20230
                     /locus_tag="KLF07_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBD9832471.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4763 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12481 atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt cctgatttta
    12541 tcaaaattga tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt
    12601 aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact acgcaatcgg
    12661 gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat
    12721 gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg catggcagga
    12781 agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc
    12841 gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaggtact
    12901 gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac gtgaagaaga
    12961 tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc cggaagagag
    13021 cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac
    13081 cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg ggcgtcctgc
    13141 cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga gcgccctgag
    13201 cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac aggcgcgcgt
    13261 ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc cgctggaaaa
    13321 tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc agccacaccc
    13381 gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt
    13441 taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca ctggcgataa
    13501 ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg agctggtggt
    13561 agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg
    13621 ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact accgccctaa
    13681 cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga
    13741 ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca
    13801 gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagtga
    13861 atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag gcttatctga
    13921 cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg
    13981 aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat
    14041 gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt gctggcggat
    14101 gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc
    14161 ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat
    14221 ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac cggcctgccg
    14281 atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga atttttcgcg
    14341 gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac
    14401 acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt accgtttatg
    14461 accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt actgaaaatg
    14521 tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga agccgatatt
    14581 ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga tgcgctggtt
    14641 cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg gcgcaacatc
    14701 atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc gccggtgctg
    14761 atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac catcttctcg
    14821 atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag catctacccg
    14881 gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat
    14941 ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt ctccggctat
    15001 atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga taaaccgtcc
    15061 gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct ggcagacgtt
    15121 gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg
    15181 tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg cctcgcgtcg
    15241 tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagctggc
    15301 atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg cggcgtaaaa
    15361 gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag
    15421 aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa
    15481 gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg aactgccagg
    15541 gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca gcaagcgttg
    15601 ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg tcaacagtgc
    15661 ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca
    15721 ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc agcttattgc
    15781 cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca gcctgaaact
    15841 ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact tcaaagtggt
    15901 ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc
    15961 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    16021 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    16081 gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc agttcaaaga
    16141 gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16201 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16261 caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgcttcccgc
    16321 gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc gcgaagaagc
    16381 cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg gtatttttcc
    16441 gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg ccgacgggca
    16501 gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca gcctggaaag
    16561 ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg ccgccctgat
    16621 ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga cgctgaattt
    16681 taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg tggcgctgcg
    16741 cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc gcagccgcat
    16801 ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct gatcgcccag
    16861 cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct ggattacccg
    16921 gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct gaaaatcgcc
    16981 gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat gcaggcaagc
    17041 tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt ccacgatggc
    17101 agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct ggctttcgtg
    17161 ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa cgccacggac
    17221 tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga tccggaaacc
    17281 cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc cggggtgatc
    17341 ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc cgccctgatg
    17401 tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa tcatccggca
    17461 aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact ggaagccggg
    17521 caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg cgacaccttc
    17581 cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg agagaacgat
    17641 ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat tatggctggg
    17701 gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact ggttgttgat
    17761 cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac aggcgattgc
    17821 gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc aaatcaaaca
    17881 actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg ctgacgaagc
    17941 ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg tgggcagtgc
    18001 gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag ccaacgatca
    18061 aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac cgcagattct
    18121 ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg ccgatatggg
    18181 ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg cgatcgtgca
    18241 gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac tggcaaaacg
    18301 ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc tgctcgcccg
    18361 cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga agcccggcgt
    18421 gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac cgtaggccgg
    18481 ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc ggataaggcg
    18541 ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta gaggaagaaa
    18601 aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc agcataaagc
    18661 gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga gcgtctttct
    18721 gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg ccggactcac
    18781 gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca ccctgttcta
    18841 tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg tcggtgcgcg
    18901 gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca ccatgtttgc
    18961 cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg aagccggctt
    19021 tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc gcgcccgtgc
    19081 caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga tcgtttccgg
    19141 ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc tgtttttgct
    19201 ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg atgactcacc
    19261 ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga tgatggataa
    19321 cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca tgcaacaacg
    19381 cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg cgtatttctg
    19441 cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc aaagttttaa
    19501 tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga tttgtaccat
    19561 tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa ggcatcacac
    19621 cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa ctgatcacaa
    19681 catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg caatggcgat
    19741 tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg gtattgcggt
    19801 gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg gctggttgaa
    19861 agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc tggtgattgg
    19921 tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga ccctgtaagg
    19981 aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt acgatgaaag
    20041 cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat tttttggccg
    20101 ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca atagcggaca
    20161 gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt ttgtcctgac
    20221 gccgccgtca
//
LOCUS       DAEEBB010000074        20147 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEEBB010000074 DAEEBB010000000
VERSION     DAEEBB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.074"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="KLF07_002473"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="KLF07_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBD9832472.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="KLF07_002474"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="KLF07_002474"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBD9832473.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="KLF07_002475"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="KLF07_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBD9832474.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /locus_tag="KLF07_002476"
     CDS             3608..5233
                     /locus_tag="KLF07_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBD9832475.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="KLF07_002477"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="KLF07_002477"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD9832476.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="KLF07_002478"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="KLF07_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="KLF07_002479"
     CDS             complement(7691..8902)
                     /locus_tag="KLF07_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBD9832477.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="KLF07_002480"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="KLF07_002480"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBD9832478.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="KLF07_002481"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="KLF07_002481"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBD9832479.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="KLF07_002482"
     CDS             11880..13658
                     /locus_tag="KLF07_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBD9832480.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="KLF07_002483"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="KLF07_002483"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBD9832481.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="KLF07_002484"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="KLF07_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBD9832482.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="KLF07_002485"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="KLF07_002485"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBD9832483.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /locus_tag="KLF07_002486"
     CDS             17775..18530
                     /locus_tag="KLF07_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBD9832484.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="KLF07_002487"
     tRNA            18609..18678
                     /locus_tag="KLF07_002487"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18871..>20147
                     /locus_tag="KLF07_002488"
     CDS             18871..>20147
                     /locus_tag="KLF07_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832485.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4981 a         4765 c         5179 g         5222 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcatag ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAEEBB010000075        20077 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEEBB010000075 DAEEBB010000000
VERSION     DAEEBB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.075"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            59..568
                     /gene="radC"
                     /locus_tag="KLF07_002489"
                     /pseudo
     CDS             59..568
                     /gene="radC"
                     /locus_tag="KLF07_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="KLF07_002490"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="KLF07_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBD9832486.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="KLF07_002491"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="KLF07_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBD9832487.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="KLF07_002492"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="KLF07_002492"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBD9832488.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="KLF07_002493"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="KLF07_002493"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBD9832489.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="KLF07_002494"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="KLF07_002494"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBD9832490.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="KLF07_002495"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="KLF07_002495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBD9832491.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="KLF07_002496"
     CDS             5387..6511
                     /locus_tag="KLF07_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBD9832492.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="KLF07_002497"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="KLF07_002497"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBD9832493.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..8333
                     /gene="waaO"
                     /locus_tag="KLF07_002498"
                     /gene_synonym="rfaI"
     CDS             7317..8333
                     /gene="waaO"
                     /locus_tag="KLF07_002498"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBD9832494.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8350..9345
                     /locus_tag="KLF07_002499"
     CDS             8350..9345
                     /locus_tag="KLF07_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HBD9832495.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9355..10047
                     /gene="rfaY"
                     /locus_tag="KLF07_002500"
     CDS             9355..10047
                     /gene="rfaY"
                     /locus_tag="KLF07_002500"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBD9832496.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10073..11098
                     /locus_tag="KLF07_002501"
     CDS             10073..11098
                     /locus_tag="KLF07_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HBD9832497.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11183..12166
                     /locus_tag="KLF07_002502"
     CDS             11183..12166
                     /locus_tag="KLF07_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD9832498.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12212..13465
                     /locus_tag="KLF07_002503"
     CDS             12212..13465
                     /locus_tag="KLF07_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBD9832499.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="KLF07_002504"
     CDS             complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="KLF07_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBD9832500.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="KLF07_002505"
     CDS             complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="KLF07_002505"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBD9832501.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="KLF07_002506"
     CDS             complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="KLF07_002506"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBD9832502.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16712..17908
                     /gene="kbl"
                     /locus_tag="KLF07_002507"
     CDS             16712..17908
                     /gene="kbl"
                     /locus_tag="KLF07_002507"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBD9832503.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17918..18943
                     /gene="tdh"
                     /locus_tag="KLF07_002508"
     CDS             17918..18943
                     /gene="tdh"
                     /locus_tag="KLF07_002508"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBD9832504.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19182..>20077
                     /gene="waaH"
                     /locus_tag="KLF07_002509"
     CDS             19182..>20077
                     /gene="waaH"
                     /locus_tag="KLF07_002509"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBD9832505.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5422 a         4377 c         4738 g         5540 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactatgct cgttattgcg ccaagcataa ttttaaacaa aatgaaccaa
     8281 ttaacgggat aatgaattat atttattatt tttatttaaa gataataaaa tgatattgag
     8341 aaaagttaaa tgaatgaatt tataaaagaa cggttttcgt atttagcaga taataaaaaa
     8401 gaaaacgccc cagaactaaa tgtttcctac ggtatcgata agaatttttt gtatggtgct
     8461 ggcgtttcaa tttcttccgt tttgattaat aattcagata ttaattttgt ctttcatgtt
     8521 ttcactgatt atgtggatga tgattattta aagtcattta atgaaacagc aaaacaattt
     8581 aatacctcaa ttattgtata tttaattgac ccaaaatact ttgctgatct gccgacgtca
     8641 cagttttggt cgtacgcgac atacttcagg gtattgtcct ttgaatatct gagtgaaagt
     8701 atttccacac tgctgtatct ggatgccgat gtcgtttgta aaggaagtct gaagcctctc
     8761 acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatgt tagtactcag
     8821 gcggcatgtg caaaacgcct caacattccc gagatgaatg gacgttattt caatgcaggc
     8881 gttatctatg tcaatattaa aaaatggcat gaagcaaatt tgacaccgta tttactcacg
     8941 cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat
     9001 atcgcattta atatgaataa tatctacctc gcgaaggatt ttgatactat ttataccctg
     9061 aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca
     9121 gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg ttatccgtct
     9181 gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag
     9241 gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca tggtgagtat
     9301 attaaaggca taacttcatt aattaagtac aagcttaaga aataaattac tcttatgatt
     9361 acaagtatac gctatcgcgg cttctcattt tattacaaag ataatgataa taaatataaa
     9421 gaaatctttg atgaaatact ggcctataat tttaaaacag taaaagtatt acgtaatatt
     9481 aatgatacca aagtatcatt gattgatacg aaatattgtc gctatgtttt taaagtcttt
     9541 gccccgaaaa cgaaaaggaa tgaacgtttt ttaaaatctt ttgtaaaagg tgactactat
     9601 cagaatttga tcgttgaaac cgatcgtgtc agaagtgcag gtcttacttt tcctaatgat
     9661 ttttattttc tggctgagcg taaaattttc aactatgcca gtgtttttat catgcttatc
     9721 gagtatgtcg agggcgtaga gctcaatgat atgccgatca ttccagaaga tgttaaagca
     9781 gaaattaagg cttcaatgga aaaattgcat gccttaaata tgctatcggg cgacccacac
     9841 cggggtaact ttatcgttag taaagatggt gtcaggataa ttgacctatc cggtaaaagc
     9901 tgtacggcag agcgtaaagc tcgtgatcgt ctggccatgg aacgtcacct gggtattgcc
     9961 aacgaaataa aagattatgg ctactattca gtaatctaca gaaccaaact acgcaaattt
    10021 attaaaaaat taaaaggcaa agcgtaaacc acacagtcaa aacggaacca acatggattt
    10081 attagctgag agtattactg aagtcgctgt ctctggggaa attgctaaca ccgatcgtgt
    10141 gttaaatatc gcttacggta ttgaccgtaa ctttttattt ggtgcggcag tatctatgca
    10201 atcagttgtt atgcataatc cggaccttgc ggttaagttt catctcttta ccgactacat
    10261 tgatgaagat tatctacaac gtgttaatgc ttttaccagc aaaaatgcta acgttgaagt
    10321 aagaatttat aaagtctcca gtgcctttat tgatatcttc cccagcctga aacagtggtc
    10381 ttatgcaaca ttcttccgtt tagttgcgtt ccagtatctg agtgaaacta ttgaaaatct
    10441 gttatatatc gatgctgatg tcatctgtaa aggctcatta gctggattgc ttgatattaa
    10501 ttttgatggg gataagtttg cagctgttat taaagatgtg ccttttatgc aggaaaaacc
    10561 agcgaagcgt ctggctatag agggacttcc agggaattat ttcaacgccg gtgtagtata
    10621 tctgcagctt gaagcatggg cgaaaaatga ttttatgaat aaagccattg ctatgctggc
    10681 aagtgacccg cagcacacga agtataaatg ccttgatcag gatattttaa atattctgtt
    10741 ctttggtcat tgtattttta ttagcggcga ttatgattgc ttttatggca tcgattatga
    10801 gttaaaaaat aaaagcgatg aagattataa aaagaccatt accgatgata ctaagctgat
    10861 tcattatgtt ggcgtaacga agccctggaa cgactggacg aattatccct gccagaagta
    10921 ttttaatgag gcttatcagg cttcttgctg gaatgatgtg gcgtttattc cagccacgaa
    10981 tgaaaagcag tatcaagtga aatatcaaca tgcaaagaaa aatggtgata cgtttaacgc
    11041 ttttatttac tttattaaat ttaaattaaa taagtataaa agaaaactat ttggataata
    11101 ataaagatca tggatgtctg agttacaata ataattaagg atgagtacta tcatatttta
    11161 ttagttcagg caagagaaca cgatgagcaa tgattaccct ttagtatcca taataatacc
    11221 gacgtataat tcatctgatt acattactga aactctaacg aaattagaaa aacaaactta
    11281 cccaaatttt gaaattgtta ttgttaatga tggttctaaa gataacacat caaacgtttt
    11341 gagagagtat gggttaaccc actctcgatt aattattatc aataaagaaa atggcggtgt
    11401 ttcgtctgcc aggaatacag gtatccgcaa ggcgcaagga cagtttatat gttttatgga
    11461 tgatgatgat gagatagatc ctaactatct gctgaagatg tataccagac aacatgagac
    11521 gggaggggat gcgatttatt gtgggcttta tggccattat ataaaagatg gtgttactta
    11581 ctcacctata aatacagagt ttaatgaagg atctttactt ttcgactttt tttataaaaa
    11641 ggttagattc catatagggt gcttgtttat aagaaaacaa cttctggaag agaataatct
    11701 tttttttgat gaagatttac gactaggaga agatctggat tttatctatc gactgctaat
    11761 tacatgcgat atgtatgcgg ttccatatta tatgtataag cataactata gagaaaattc
    11821 cttaatgaac tcatgtagaa ccatcactca ttatcgacat gagtcatttg cgcacgaaag
    11881 aatctactct tctgtgatgc agttatacaa aggtaacagg aaagaagaaa ttcatacatt
    11941 attgagtcaa aatagagctt atcataaaac tcgttattta tggaatgttc tacttaatgg
    12001 tgattttgaa ctattaaatc aattagttga aagcaatgaa aaagaattga atgattgtaa
    12061 tctttctggc aagagagata aaagacgagc aaaaatatta gcatcaaaaa attatattct
    12121 ctggaggatg gtaagactgg taaataggaa aaagaataaa cgttagatct tgcattaaat
    12181 aatccgtcct cgtagcatag gttgaaacta tatgtcattt tgttggaatg aaattaattc
    12241 tggtataaag tctttaattc tcatattatg tattttttct ttgatgactt tgtctttatg
    12301 ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt atatctgcat tgtattttct
    12361 aataacacca aaaaaaacaa taactaacaa tcctactttg ttaattttca tctcattatg
    12421 tcttttggga atcataaata tcatctggta ttcacattat aaagtttcag gctctgttta
    12481 taccaatgca tatcgtggcc caatggaaac tgggaaaatt gccttgtgta gtgcttttat
    12541 tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg ggaaactaat
    12601 tctgttcgca tccctggcaa cgcagttact tttttttgcg catgccatgt ggcaacattt
    12661 ctatttaaac gttgaccgtg ttgcattatc agcttcccac gctacaacag caggctacat
    12721 catccttttc ccttctttac tggcatcaat tctcatttta aaatccgact ttagacataa
    12781 aacaacatta tatacaatta acttcatgct tagcttatgt gctgtcatag taactgaaac
    12841 gcgtgcagcc atattagtgt ttccattctt tgcgttacta ttaatcgtaa tggatagtta
    12901 tattaataag cgaattaatt ataagttata ttgttttatt gcgattgcat tattagcagg
    12961 tgtattttct tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt
    13021 taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt
    13081 tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca
    13141 tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt
    13201 gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat
    13261 tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt
    13321 aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc
    13381 ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc
    13441 agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat
    13501 tttatttcaa aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa
    13561 tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa
    13621 ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga
    13681 tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa
    13741 atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa
    13801 cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg
    13861 gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt
    13921 ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat
    13981 ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt
    14041 tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt
    14101 ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc
    14161 agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca
    14221 gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt
    14281 tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag
    14341 gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca
    14401 cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat
    14461 ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct
    14521 cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc
    14581 tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc
    14641 ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca
    14701 gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga
    14761 caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca
    14821 ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt
    14881 catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg
    14941 ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc
    15001 aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt
    15061 caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac
    15121 gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt
    15181 ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg
    15241 tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga
    15301 cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga
    15361 tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg
    15421 ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg
    15481 cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga
    15541 tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg
    15601 ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca
    15661 gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt
    15721 cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg
    15781 cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca
    15841 catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca
    15901 gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc
    15961 tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg
    16021 agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt
    16081 agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt
    16141 aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca
    16201 gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg
    16261 aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa
    16321 tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca
    16381 caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat
    16441 tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa
    16501 ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc
    16561 actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc
    16621 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
    16681 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
    16741 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
    16801 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
    16861 taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga
    16921 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
    16981 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
    17041 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
    17101 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
    17161 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
    17221 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat
    17281 tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga
    17341 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
    17401 tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc
    17461 tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc
    17521 gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga
    17581 gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg
    17641 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt
    17701 catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
    17761 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
    17821 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
    17881 tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa
    17941 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
    18001 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
    18061 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga
    18121 agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga
    18181 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
    18241 caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc
    18301 ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga
    18361 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct
    18421 ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc
    18481 acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg
    18541 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
    18601 catggccgaa ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac
    18661 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
    18721 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
    18781 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
    18841 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
    18901 gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt
    18961 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
    19021 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
    19081 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
    19141 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
    19201 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
    19261 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
    19321 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
    19381 atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca
    19441 aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga
    19501 tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc
    19561 gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat
    19621 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
    19681 ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat
    19741 tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat
    19801 ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata
    19861 gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag
    19921 agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa
    19981 taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc
    20041 gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAEEBB010000076        19771 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEEBB010000076 DAEEBB010000000
VERSION     DAEEBB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.076"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(82..2223)
                     /locus_tag="KLF07_002510"
     CDS             complement(82..2223)
                     /locus_tag="KLF07_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBD9832506.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(2433..2951)
                     /locus_tag="KLF07_002511"
     CDS             complement(2433..2951)
                     /locus_tag="KLF07_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBD9832507.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            3648..4148
                     /gene="tssB"
                     /locus_tag="KLF07_002512"
     CDS             3648..4148
                     /gene="tssB"
                     /locus_tag="KLF07_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBD9832508.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            4183..4407
                     /locus_tag="KLF07_002513"
     CDS             4183..4407
                     /locus_tag="KLF07_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832509.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            4458..5933
                     /gene="tssC"
                     /locus_tag="KLF07_002514"
                     /pseudo
     CDS             4458..5933
                     /gene="tssC"
                     /locus_tag="KLF07_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            5940..6353
                     /gene="tssE"
                     /locus_tag="KLF07_002515"
     CDS             5940..6353
                     /gene="tssE"
                     /locus_tag="KLF07_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBD9832510.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            6357..8207
                     /gene="tssF"
                     /locus_tag="KLF07_002516"
     CDS             6357..8207
                     /gene="tssF"
                     /locus_tag="KLF07_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBD9832511.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            8171..9253
                     /gene="tssG"
                     /locus_tag="KLF07_002517"
     CDS             8171..9253
                     /gene="tssG"
                     /locus_tag="KLF07_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBD9832512.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            9278..10558
                     /gene="tagH"
                     /locus_tag="KLF07_002518"
     CDS             9278..10558
                     /gene="tagH"
                     /locus_tag="KLF07_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBD9832513.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            10555..11079
                     /gene="tssJ"
                     /locus_tag="KLF07_002519"
     CDS             10555..11079
                     /gene="tssJ"
                     /locus_tag="KLF07_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBD9832514.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            11082..12411
                     /gene="tssK"
                     /locus_tag="KLF07_002520"
                     /pseudo
     CDS             11082..12411
                     /gene="tssK"
                     /locus_tag="KLF07_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            12416..13177
                     /gene="icmH"
                     /locus_tag="KLF07_002521"
     CDS             12416..13177
                     /gene="icmH"
                     /locus_tag="KLF07_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBD9832515.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            13186..15969
                     /gene="tssH"
                     /locus_tag="KLF07_002522"
                     /pseudo
     CDS             13186..15969
                     /gene="tssH"
                     /locus_tag="KLF07_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            15966..16709
                     /gene="tagO"
                     /locus_tag="KLF07_002523"
     CDS             15966..16709
                     /gene="tagO"
                     /locus_tag="KLF07_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBD9832516.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            16714..18126
                     /gene="tssA"
                     /locus_tag="KLF07_002524"
     CDS             16714..18126
                     /gene="tssA"
                     /locus_tag="KLF07_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBD9832517.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            18145..>19771
                     /gene="tssM"
                     /locus_tag="KLF07_002525"
     CDS             18145..>19771
                     /gene="tssM"
                     /locus_tag="KLF07_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBD9832518.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         4523 a         5474 c         5352 g         4422 t
ORIGIN      
        1 gcccatgaat caattccttc tgcttttaat atggttcatc tggtcactgc accagttccg
       61 ttttaatttt ttgcacctga ctcaaaattg ccgcatatcc ggcattccgg ggggtaaatc
      121 tttcggcaca ggcgcatccg gtacaggttg tgtggcacca cagttccagt taatcactgg
      181 cgcatcaccg ttcacatccg actccccttc cagatgaatg tgcgtaccgt tgagaaatat
      241 gcttccgtcc ttcgtcagca ccagccttgc cttaccacag cagagttcaa gatgctcacc
      301 ggcagaataa actgctgttt gtccggtgtt ttccttcatc gtcttgccca ccgagaaggt
      361 gacgttattc cctacattga cgctgtagcc cattcccacc atcagtgatt tatgcagccc
      421 tacctgtgag gactgcaaca acgccaccga ggtattcata atgccgccta ccgtcgtctg
      481 gtaagccaca cccaccgtca gcgcacgaat gatccccacc ttttcaacct ggtttgatcc
      541 cactttgata atctggttac tgcccaccgt ttccaccagg ttgacaccaa ccgtctgtat
      601 ctgattaacg ccaatgttct ggatctggtt attggtaacg gtgatcgcct ggttgttacc
      661 tattttttcg gcatggttgt ttatcacatc cgtggtgcgg ttattcagca cctcggtgtt
      721 catgttcttc tgcacgtgga tgtagacctg ttctttccct gtcgcatcgt caaacttcag
      781 ttcattaaac ccgctgccct tataggtttt cgaacgaatg gtcatctgcg tcttcgtccc
      841 cggcaggcta ccgggtgtgc ggttttcctg gtggtaggta cgccccataa taatcggctg
      901 gtccggatcg ccgttgagga agtccacaat gacctcctga cccacgcgcg gtatcgcaag
      961 gttaccgaat ccggtgcctg cccacgcctg tgccacacgg atccagcatg aactgtcctg
     1021 gtttgacggg ttatagcggt cccagttaaa tttcacccgc acgcggccat gttcatcaca
     1081 gaagatttcc tcgcctgccg gtcccgtcac gacggcgctc tgcgggccat ccaccagcgg
     1141 tttcagcaac ggctgtggcc gccatgtccg gtctgccggt attaccgcaa agtggttatt
     1201 cagcgtggtt cctgaaccac tgcgtcccgg caccgcctgc ggctgctcgc cgtgcagttc
     1261 acttgccacc acctgccatt cccggttcag gttcgcctgc ggatgccccg tcagcacaat
     1321 tcgccgtccc ggccatatct ccggagaacg gcttgttccg cgcgccactt ctgcgttgtt
     1381 gcgccagcca tccatctgcc agcgggcaaa gttctgcccg tgagcaccct tgaaacgtcc
     1441 ggggtagtca tacacttcat actgtgtgcg ctggtaatcc tggtactggc cttcctgatc
     1501 aaaacgccct gcccagccgg gtcgtttaaa ggtgtagtct ttggtcacca cggaagaagg
     1561 acggatttgt gcgctgtagc ggaactggct gatgcagaag gtacttacct cggtacgggt
     1621 gttcgggttc caggggatct caaaggactc cggcagataa cgcacggtgt cgcacaggac
     1681 caggctctgg tcggtacttt tttgcgcgtg ctcctcataa aagaagatgc cttcctccgc
     1741 cgccatccgg cacaggaaat cgtaatcagt ttcgccgtac tggacacaaa actcacggga
     1801 aggatgcggc tcgctgaaca gcggactcca ctcggtcaca ccgttttcct tcaggatcgt
     1861 cgcgaggatg ctttcgatgt cctcattctg gaagatacgg aagttctggc gcagccctgt
     1921 gcgccacagc ggtgggcata ccttcatgct gtacagcatc tggtttttgt cgttctcccc
     1981 cagttcaaac caggtcacca cacctttcac ccggcgctgt acgtcatcgc cctgccatat
     2041 cgtcaggtag gccattttgt ccagtacctg cggaaattca agggagagaa actgctggct
     2101 gaccagagag agctcgaggg aaaagagcga agagagtgac tggttcagat gaaaggagac
     2161 caccgcaaaa gcatccggtg gcaggccgtc cacttccagc gtgaaacgta atccggttga
     2221 catagcaacc tccggggtta aggaaaaaca gacagttcgc tgttaaagcg gctgtgacag
     2281 tacagtgtta cgcttgcgtt caggcacgct accgccgaag tttccggcat ccgccatctc
     2341 ccgtgaacgt aaccccttac tgacaacctt tctttctacg gcgtaataca aaaacagcat
     2401 ccggcctgat agccggatgc tgttggctta acttatgctt ccagtggtgc gcgccagtcg
     2461 tcggcaccgg aggtgccaga tttgacgtgt tcccattcaa ttttgcggta ggccagtgaa
     2521 acctcgagca actgggtgaa ctcgcgtttc gccgggtcct ggcaatgcgg catatgcagt
     2581 ttcatatcga caatcgtcga atcagtcagg cgggtagtga aataatgctc ctgtttgccc
     2641 tccacagagg tacgccacca gtgaagttca gtggttggca gcatttcgcc ggaggccagt
     2701 gcgttgtaca tcagcggaac cgctttgttc agcgccacgg taaaaatgaa aggcttgtga
     2761 gcacgctgac ccgcaggctg accggactga ggagtggtcg ggacagtaac gttatgcagg
     2821 aattcctgca ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc gacagaatca
     2881 gcggtaaatg cacctgcagt aatatttccc tgggtctgac cagtgatgga gatataacac
     2941 ggtgttggca tttgtaaact ccttgttaac gtggttaatt actacttaca atgacctgcc
     3001 gttaatccca gacaggcaga tgcagctcac ttcacggaac cgcatcattt ttcagtattt
     3061 tttacataca aaccgagcta gaatttacgg ttgtcgtaaa aactattttt aaaatcatta
     3121 aatctcttat tcagcaataa cttaataata tatatctgga taaccatacc caactgttca
     3181 aagtccgtat tgcctccata aaatggccaa agctgaatca tgaataaata ttgtatttat
     3241 cctttctgaa ttatcatttt gataattttt gagatattcc ttaaaaatac ccacttaatc
     3301 aatcacaaaa caaatatata aacaagcaat cattagatta gcttattttt aatgaaatta
     3361 ttatatactt caataagtta cacaaaaact ctatatttaa attattgcca taatacacct
     3421 gaagcgaatt gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg
     3481 atcgataaaa agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag
     3541 tggtgatttt attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa
     3601 tatgccgtgt tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat
     3661 ttgaaggcag cgtcgcgccc cgcgagcgta ttaatatatc gtatgtccca aaaacagatg
     3721 gccagacggc tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata
     3781 cgcaggaaac atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg
     3841 gtgcagtgat ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg
     3901 gaagcacaac agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt
     3961 caccagatag cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag
     4021 cactgactgc actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg
     4081 cattgctgga aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca
     4141 ataaataacc accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat
     4201 atctccggga cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt
     4261 ttcagcccgt ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat
     4321 cgccgtatca gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg
     4381 tcgcactggc agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa
     4441 acagaaggat tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc
     4501 agcctgagca ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg
     4561 gttcggaagg ttatgacgtt gccctccagg gtgtgacagc ctttattgcc agcatcctgc
     4621 aatccactgc atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata
     4681 ttgatgaacg catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg
     4741 tcgagtcgtt ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca
     4801 tcaaggtcaa cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc
     4861 cagaaattat tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg
     4921 gcggtaaacc catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca
     4981 tgaaactgct gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct
     5041 ccgtttcccc ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc
     5101 tttatgccat ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag
     5161 attcgcgcta tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa
     5221 cagataaccc tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt
     5281 atctgtgggg taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat
     5341 accgctgatg tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccga
     5401 tgcatctgtt cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca
     5461 ccgatcgccg cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag
     5521 actctgataa tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg
     5581 gaaaagatgc agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca
     5641 atcggttagc gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa
     5701 ggagcgactt agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa
     5761 acccgcctgc tgacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg
     5821 atgtagaagg cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat
     5881 ttatgggggc aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca
     5941 tgataccgga tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg
     6001 ccagaaaccg gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac
     6061 gaaacattct taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg
     6121 aaccggaagg ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag
     6181 aacgctatga accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca
     6241 gcccgctgga tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg
     6301 tgcttgaatt tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg
     6361 aatttgaaga acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc
     6421 tggcaacgga aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata
     6481 ttgaacgcct gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg
     6541 atgaattccc agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc
     6601 cggttccggc aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg
     6661 tgttaatccc ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag
     6721 tgctgccctc tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg
     6781 atatctggct gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg
     6841 gtgttatcaa catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga
     6901 acaaacttcg cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat
     6961 ggttctgcga atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc
     7021 ctgagtttat gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga
     7081 aaaacgtcca cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc
     7141 tcttttttga tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta
     7201 ccctgcaact gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc
     7261 tgcgcctgta ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc
     7321 tggacaaccg gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg
     7381 atgtattttc cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc
     7441 tggggcgtcc tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc
     7501 atcagataga atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat
     7561 ccctgttcca tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc
     7621 cgtcagacga atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc
     7681 gtgagcttcc gtcacaaatt cgttccggcg atatcaccgg cacaaccgga aaaaatgcgg
     7741 ctgttgcttc atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg
     7801 gcagcctgca ctggccccta ctctccgcca tgaacctgaa ttatctgtca ttactggata
     7861 cggacgcgct gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga
     7921 cggcacggct gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg
     7981 atcgcctgtt tacgggtatt cccgtccggg ggctggcttc cacgctgtat ctgcacccgg
     8041 agccgtttgt ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt
     8101 cgctgtacgc cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg
     8161 agacatggaa atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc
     8221 cccggcagat aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta
     8281 caattttttt gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc
     8341 agaattatcc ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag
     8401 ccttgccttc ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag
     8461 gatgaccacc acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct
     8521 cgctcacctg gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat
     8581 gttcagccac cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat
     8641 cagttttcgt aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct
     8701 gggccaccgc cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc
     8761 cgggatcctg gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg
     8821 ctttgatttg tcagaagtga cactgcaaaa ctggcaacgt cgcaaggtgg atattgaacc
     8881 ggatcagcaa aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag
     8941 atcggtactg ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca
     9001 gctcagtatt accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa
     9061 cttcctgcca ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt
     9121 acatctgggg ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc
     9181 gctgctgggc tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag
     9241 ggtacggtcg taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac
     9301 gctttcattg caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct
     9361 gttcacacag cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg
     9421 tcagcagagc attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct
     9481 gcgcccgcag acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct
     9541 aatacagttg cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct
     9601 gaaccgggga gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac
     9661 caatcgcgat atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg
     9721 aatgactcac cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact
     9781 ccaggctctt cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc
     9841 tgtccggcca tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt
     9901 tatggatctg ccgcccattt atgccagccc cggcgatcgt aatgacgacg tttctgcggc
     9961 agaaatggca caacgccacg ttgcggtcac ccccttactg cgcggtcttg gcggctcgct
    10021 taccatgagc aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca
    10081 ggccgcaata aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca
    10141 tttgcgcccg ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga
    10201 cgtaatgttt gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga
    10261 aagcctgcgc aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct
    10321 tcgtgtcctg ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg
    10381 ccgcagccat gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca
    10441 ttattacgaa gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt
    10501 ctacgcccag gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac
    10561 aggaaggcgt ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg
    10621 aaaaaaatca gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa
    10681 ccttcggaaa taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt
    10741 gaatcagccg ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca
    10801 gccgactatg accagatagc cagcacgccg ctaccggatg tgctgggtaa aaactatatc
    10861 gaccaccagg atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg
    10921 gatgagaaaa cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa
    10981 tggaaacaaa tcgagacggt ggaaggaatc ggccaccatt accgcctgct ggtgcatgta
    11041 cgtcaaagca gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca
    11101 aggtgatgtg gcaggaaggg atgctgatgc gtccacacca tttccagcag cagcagcgtt
    11161 acaacgacta cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta
    11221 ccgaactcac cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt
    11281 caggcacact gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc
    11341 cgctgcaccc gcaaaatttt ccggacgaaa gaagccgcaa tatctacctc gctctgccgg
    11401 tcgccagtga tgtgagaaat gaaatcagcg acggacggcg aatcgggcgt taccggctga
    11461 attatgccga tgtccgggat ttgcattcag aagaaggtga cgcgcgaaca ttgacgctgg
    11521 gtcaactgac gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc
    11581 cgctttgccg tatcagcgat cgccgccgat ggctccctga cgttggatga cgattttatc
    11641 ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag
    11701 ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc
    11761 ggcatcgcgg atgtggcgga atttatgatg ttgcagttac ttaaccgtaa ccagacccgg
    11821 tttacccatc acgcccgtcg atcccagctc cacccggaag atttctacct tgatcttgcc
    11881 gggttgctgg gtgaactgat gacctttacc gagccgtcgc gcctgccctg cccgcttgat
    11941 gtgtatgatc atcatgacct gaccaaaata tttaaaacgc tgttaccgga agtcaaacgg
    12001 gcgctgcata ccgtactgtc gccaagagcc gtcaatctgc cgctgcatct gcgtgacggt
    12061 atctggcagg ccgatatcca tgacacagaa ctgctgcaat ctgccacctt tgtgctggcc
    12121 gtggcggcaa acatgccggt cgatcagatc cagcgccagt ttatccagca gtcgaaaatt
    12181 tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tccccggtat tccattgcgt
    12241 gcccttatgg tggctccccg ccagcttcct taccattccg ggttcagcta tttcgaactc
    12301 gacaagagtg gccaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgtt
    12361 tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga
    12421 tgaggggtca ctgtctctgc caccatttac cggacacgac gaaaaaagcc agcgcaacta
    12481 ccatctcgcg cttcgtggca acagcctgaa tccgatgatc gacgcggcca cgccgctact
    12541 cggtatggta atgcgccttt caaccatgaa cagccagacg atgccggagc atctttttgc
    12601 tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggagcagg gctacgaacc
    12661 gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg
    12721 taacggctgg tcaaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa
    12781 cgaagcctgg ggtggcgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa
    12841 acgttaccag gatctgctgg aatttctctg gatctgtttt tctctgggat ttcgcgggcg
    12901 ctacaaagtg gcagcacagg atcaggggga atttgagcag atctaccgcc gtctgtacca
    12961 cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac
    13021 ccagggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt tttgtggtgg
    13081 cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca
    13141 ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat ccagattgat
    13201 cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc
    13261 tctgaatgta tgagtcagca ggcagcggaa attaccgtca gccatgtgct cattcagatg
    13321 ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat tggcatggaa
    13381 gagttgcgcc aggcgctgac ggtagagaac tacacaaccg cccgttctgc ggacagctac
    13441 ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctagctgct ggcgtcggct
    13501 gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccctgct gcattcgccg
    13561 ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg
    13621 caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac
    13681 ggtaaaggcg caggcacaat gacggacgcc tctgacaccc tgcttgcccg ctatgccaaa
    13741 aacatgaccg cagacgcccg taacggcagg cttgacccgg tactgtgccg cgaccacgaa
    13801 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccagt ggtggtgggc
    13861 gaagcgggcg tgggcaaaag cgcactgatt gaagggctgg cgctgcgcat cgtggcaggc
    13921 caggtgccgg acaagctgaa aaacaccgat atcatgaccc ttgacttggg cgcattgcag
    13981 gccggggcgt cggtgaaggg tgaattcgaa aaacgtttca aagggctgat ggcggaggtc
    14041 atttcctccc cggtgccggt cattctgttt atcgacgaag cacataccct gattggcgcg
    14101 ggcaaccagc agggcgggct ggatatctcc aacctgctca aaccggcgct ggcgcgcggc
    14161 gagctgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgaaaaagat
    14221 gccgccctgt cgcgccgctt ccagttggtg aaggtcagcg aacccaacgc tgccgaagcc
    14281 accattattc tgcgcggtct gtcggcggtc tatgaacagt ctcacggcgt gctgattgat
    14341 gatgacgcct tgcaggccgc tgcgacatta agcgagcgtt atctctccgg gcgtcagtta
    14401 ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtcg
    14461 tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga ggcggaaatt
    14521 cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag
    14581 gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc ggcctggcac
    14641 cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact gggcgtggca
    14701 gaggacgatg cggcgccgtt gccggacgca gataccgtgg aggatacgca gccagagtca
    14761 gagtcagagt cagagtcaga gtcagagcag gataataccg gtgccgaacc ggctgatgag
    14821 gccgacagag aacaaccgga agagaccgct gaaacagttt ccccggtaca gcggctggcc
    14881 cagctcactg ccgaactgga cgccctgcat aacgaccggt tgctggtctc cccgcacgtc
    14941 gataaaaaac agattgcggc ggtgattgcc gaatggaccg gcgtgccgct caaccgcctg
    15001 tcgcagaatg aaatgtcggt catcaccgac ctgacgaaat ggctgggtga caccatcaaa
    15061 ggccaggacc tggcgattgc cagcctgcat aagcatctac tgaccgcacg cgccgacctg
    15121 cgtcgtccgg gacgcccgct cggtgcgttc ctgctggctg gccccagcgg tgtgggtaaa
    15181 accgaaaccg tcctgcaact ggcagagctg ctctacggcg gtcgccagta cctgaccacc
    15241 atcaatatgt ccgaattcca ggagaaacac accgtctcgc ggctgattgg ttcgcctccg
    15301 ggctacgttg gctacggtga aggcggcgta ctgaccgaag cgattcgcca gaaaccctac
    15361 tcggtagtac tgctcgatga agtggaaaaa gcgcacccgg atgtgctcaa cctgttctac
    15421 caggcgttcg ataagggcga aatggcagac ggtgaaggcc gcctgattga ctgcaaaaat
    15481 atcgtcttct tcctgacgtc caacctcggc taccaggtaa tagtcgagca tgccgatgac
    15541 ccggaaacca tgcaggaagt acagtatccg gtgctggccg acttcttcaa acctgccctg
    15601 ctggcgcgta tggaagtggt gccgtatctg ccgctgtcga aagagacgct cgccaccatt
    15661 atcgccggaa aactggcccg tctggataac gtgctgcgca gtcgctttgg tgtagaagtg
    15721 gtcattgaac cggaagtgac ggacgaaatc atgagccgcg tcacccgcgc ggaaaacggc
    15781 gcgaggatgc tggaatcggt catcgatggc gacatgctac cgccgctctc gctgctgctg
    15841 ttgcagaaaa tggcggctaa cacggcgatt gcccggattc ggttgtcggc agtggacggc
    15901 gcatttacgg cagacgtgga agatgctcag aacgacgagt ccgtcacaaa ggatgaaacg
    15961 gttttatgag caggttgcgc gttttccggc agacaggatg gtttatcgcg ggcctgatga
    16021 cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc aatggccggt gtggcggtcg
    16081 ccgtcgccac cacgaccccg ccggatgcga cagccacgtt acaggccatg cagtcctgtc
    16141 ggcgggagtc cgcggcgctg gagcggcttg actgctatga ccgcctgctg gcaccgctgt
    16201 ccccgtcagg gtttgacggg gcgctggtca aagccggttt tgtgggcgag gcctggaccc
    16261 gcgccacaga gcaggaaaaa cgccgcgagg gcaacaccac agaactgctg gtgacgcagg
    16321 tgccggggga gcgcccgacg gtggtgataa ccaccccggc tatcggtcat gttccgccac
    16381 gcccggtgct gatgttcagt tgcgtggaca atatcacccg gatgcaggtg gcgctgatgc
    16441 accctctgga tgtccatgat atcgccgtca ctctgaatgc cgacaaccgg gcattgcgca
    16501 gccactggtt tgtgcgcgag aacggcaccc tgctggagtc cagccggggg ttatccggga
    16561 ttgatgaaat taagcaactg ttcggcgcaa aaacgctgac ggtggatacc ggcgcagaca
    16621 atgccgccgg aaagctgacc tttaacattg acgggctggc aagggcgatt gctcccctgc
    16681 gcgatgcctg ccactgggcg ggagaataaa aatatggcaa tggacttacg cgatccgaat
    16741 gtctggatat cgcacctgct ggaaaacctg ccggaagaga aactggcatc ggcgctgaaa
    16801 gatgacaacc cgaactggga gtatatcgac ggcgaaatcg tcaagctggg atcgctggcc
    16861 cacagtcaac tggatatccc ggaactccag cgccgggggc tggtcattct ggcttcggag
    16921 agtaaggatt ttcgtctgct ggcgcatctg ctaagaaccc tgcaacacgc cggtgatcct
    16981 catctcgcat tgcgcctgct ggcgctgtat gtggagcatt actgggcggt ggctgcgccg
    17041 cagaatatgg cgcataaaaa acgctttgcc agccaggtca ttaaacgctt tgaaacaggt
    17101 attgaagttt tttcacagaa cgccgccaca gcgcagcgcg atgcgctgtc aggggaactg
    17161 gcaaaactgg cgcaatgctg gcagtcacat aacgccccgg aactggcaca ggctactgat
    17221 gatctttttg ccctgtacca gcgtgcgttt aatcgtgcgg caccggctcc tgtccccact
    17281 ccggcggcct ccggtagttc accacagacc accgccacgt ctgaaagcgg cgtgacgcaa
    17341 cccagtgctc cggctcccca aatcgtcatc gacagtcacg acgacaaagc ctggcgcgac
    17401 acgctgttaa aagtggcggc cattttatgt gaacgccagc cggattcacc gcagggctac
    17461 cgtctgcgcc gccatgcgct ttggcagaac atcaccagta cgccccaggc ggaaagcgat
    17521 agccgtactc cactggctgc ggtctccgcc gatatggtgg cagattacca tgctcagctc
    17581 ggtagtgccg atatggcgct gtggcagcag gtggagaaaa gcgtactgct ggctccttac
    17641 tggctggatg gtcactgcct ttctgcacag acggcgctgc gtctgggtta caaacaggtg
    17701 gcagacgcca tccacgatga ggtcatccgc ttccttgagc gtctgcccca gcttaccggg
    17761 ctgctgttta atgaccacac accgtttatc agtgagcaga cgaaacaatg gctggcagct
    17821 tcgcccgacg ccaaagttgc accggttgcg caaatcggtg aggaatcgaa ggcagccaga
    17881 gcctgttttg ctgagcaggg tctggaggcg gcgctgcgat atctggacat gctacccgaa
    17941 ggcgatcccc gcgatcagtt tcaccgccag taccttgccg cacagttgac ggaagaggcg
    18001 gggctggtac agcttgcgca gcagcagtac cggatgttgt tcaggatggg attgcagatg
    18061 atggtggcag actgggagcc gtccctgctt gagcagctag aacaaaaatt cacggcagaa
    18121 caataaaaga gaacaggagt ttttgtgttc agattaccaa caccccgact actcagcgga
    18181 ctcaaatcag ccctgcgacc ggcgatgccc cggtttaaag tctctgcttt ctggctgctg
    18241 atactggcgt ggatttttct gctggtgtgg atctggtgga aaggcccgat gtggacgctg
    18301 tatgaagaac agtggctcaa accactggcg aaccgctggc tggcaacggc ggcgtggggg
    18361 attattgccc tggtgtggct taccgtccgg gtgatgaagc gcctgcaaca gcttgaaaaa
    18421 atgcaaaagc aacagcgcga ggaagccgtt gatccgctca gcgtggaact gaacgcccag
    18481 cagcgttatc ttgaccgctg gctgctgcgc ctgcaacgcc atctcgacaa ccgccgtttc
    18541 ctgtggcagt tgccgtggta tatggtcatc ggcccggcgg gcagtggcaa aacgacactg
    18601 ctgcgcgagg ggtttccgtc cgacattatt tatgccccgg agggcgcacg cggcgcagaa
    18661 caacgcctgt acctcacgcc ccatgtcggt aaacaggcgg tgatctttga tatcgacggc
    18721 acactctgcg ctcccgctga tgcggatatc ctgcatcgcc gtctgtggga acatgccctc
    18781 ggctggctga aagaaaagcg cgcgcgccag ccgctgaacg ggattattct gacactcgat
    18841 ttacccgatc tgctgaccgc agacaaacgc cgccgcgagc atctgttaca gacgctgcgc
    18901 agccgcttac aggatatacg ccagcatctt cactgccagt taccggttta cgtggtactg
    18961 acccggcttg atttattaca gggttttgcc gccctgttcc agtcactcaa cagacaggat
    19021 cgcgatgcga ttctgggcgt cacgttcacc cgccgtgccc atgaaaatga tgactggcga
    19081 acagagttga atgctttctg gcagacatgg gtggatcgaa tgaatctggc gttgccggat
    19141 ctgatggtcg ctcagactca cacccgcgcg tctttattca gtttttcccg ccagatgcag
    19201 ggaagccgtg aaccgctggt gtcactgctt gagggtctgc ttgatggcga aaatatgaac
    19261 gtgatgctgc gtggtgtcta tctcacctct tcgcttcagc gtggacagat ggatgatata
    19321 ttcacccagt ctgccgcccg ccagtaccgg ctgggcaata acccactggc gtcctggccc
    19381 ctggtggaca ccgcgcctta tttcacccgc agcctgttcc cgcaggcatt actcgcagag
    19441 cctaatctgg caacagagag ccgcgcctgg ctgatacgtt cccgtcgccg cctgacggtt
    19501 ttctctgcca caggcggcgt ggcagcactg ctgctcatca ccggctggca tcactattac
    19561 aacggtaact atcagtccgg catcaccgtg cttaagcagg ccaaagcctt tatggacgtg
    19621 ccgcctccgc agggggaaga tgactttggc aacctgcaac tgccgctcct gaacccggta
    19681 cgcgatgcca cactggccta tggcgactgg ggcgaccgca gccgtctggc cgatatggga
    19741 ctgtaccagg gacgacgtat cgggccttat g
//
LOCUS       DAEEBB010000077        19739 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEEBB010000077 DAEEBB010000000
VERSION     DAEEBB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.077"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="KLF07_002526"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="KLF07_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBD9832519.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="KLF07_002527"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="KLF07_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBD9832520.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="KLF07_002528"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="KLF07_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBD9832521.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="KLF07_002529"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="KLF07_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBD9832522.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="KLF07_002530"
     CDS             complement(2270..2761)
                     /locus_tag="KLF07_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBD9832523.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="KLF07_002531"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="KLF07_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBD9832524.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="KLF07_002532"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="KLF07_002532"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBD9832525.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="KLF07_002533"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="KLF07_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBD9832526.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="KLF07_002534"
     tRNA            5405..5492
                     /locus_tag="KLF07_002534"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="KLF07_002535"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="KLF07_002535"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="KLF07_002536"
     CDS             complement(5764..6450)
                     /locus_tag="KLF07_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBD9832527.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="KLF07_002537"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="KLF07_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBD9832528.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="KLF07_002538"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="KLF07_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBD9832529.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="KLF07_002539"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="KLF07_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBD9832530.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="KLF07_002540"
     CDS             complement(10902..11732)
                     /locus_tag="KLF07_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBD9832531.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12398..13906)
                     /gene="putP"
                     /locus_tag="KLF07_002541"
     CDS             complement(12398..13906)
                     /gene="putP"
                     /locus_tag="KLF07_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBD9832532.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14328..18290
                     /gene="putA"
                     /locus_tag="KLF07_002542"
     CDS             14328..18290
                     /gene="putA"
                     /locus_tag="KLF07_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBD9832533.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="KLF07_002543"
     CDS             complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="KLF07_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBD9832534.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            19256..>19739
                     /locus_tag="KLF07_002544"
     CDS             19256..>19739
                     /locus_tag="KLF07_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBD9832535.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         5013 a         5256 c         4806 g         4664 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg cccattcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaagttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
    12241 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgact ggccgccccc
    12301 tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac attaagggag cgcaacccgc
    12361 tgctgaagcc aaacaaaacc gccgcaggct aagtccctta gttttcctgc aaccgtgacg
    12421 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
    12481 gcgctttacc cagcaaacta aacactacaa tcccgatact gccgaagatg aagcccggaa
    12541 taatttcgta cagtcccagc cagccgaact gcttccagac gataactgtc agcgcaccga
    12601 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca
    12661 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
    12721 ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc caccaccagc accatcacac
    12781 gtcctaccca caccagctct ttctggctgg cctgtttacg cagaaacgct ttgtataaat
    12841 cttcggtaat cgcactggag cacaccagca gctggcaact taaggttgac attaccgccg
    12901 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
    12961 cgataaacac gcgttcagcg ttctggttta ccgcacctgc taccgccgga tgctcgttaa
    13021 agtaagcgat accaaagaag ccaacagcca ccgcccctgc caggcaaagg atcatccagg
    13081 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atctgccgcc ataaaacgcg
    13141 ccaggatgtg cggctgcccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
    13201 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
    13261 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
    13321 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
    13381 aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc cgtttcgtaa ctcatgccaa
    13441 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
    13501 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
    13561 cggtgaaata atccggcaat gttaaggcgt tattattgta ttcggtatgc acacgcaacc
    13621 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
    13681 tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc cattaacagc cagccgctca
    13741 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
    13801 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
    13861 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
    13921 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
    13981 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
    14041 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
    14101 ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt aactgccgca gagaaaaagt
    14161 ctgagttatt ttttaatcac tgtcatatcg atttctttta ttaacatttc attcattttt
    14221 aagcttgcta cgcaagtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
    14281 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
    14341 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
    14401 tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
    14461 aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgcggcc aatgagagcg
    14521 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
    14581 tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc ccggaaaccg
    14641 aagcggtttc tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc
    14701 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggtcgtg
    14761 caggaatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
    14821 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc gacgcgttaa
    14881 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
    14941 ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc acccataacg
    15001 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc
    15061 gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcacg ggtgaaacca
    15121 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
    15181 atatgctggg cgaagctgca ctgactgccg cagacgcaca ggcgtatatg gtttcctatc
    15241 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
    15301 gcatttcaat caaactgtcg gcgctgcatc cgcgctacag ccgcgcccag tatgaccggg
    15361 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtatgata
    15421 ttggtattaa catcgatgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
    15481 aaaatctctg tttcgagccg gaactggcag gctggaacgg catcggtttt gttattcagg
    15541 cttatcaaaa acgctgcccg ttggtgatcg attacctggt tgatctcgcc acccgcagcc
    15601 gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg
    15661 cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt
    15721 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    15781 cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc
    15841 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtac gagcaggtca
    15901 ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg tatttatgcc ccggttggca
    15961 cacatgaaac gctgctggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct
    16021 cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg
    16081 tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa
    16141 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    16201 ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct caatagtgca ctgcaaaaat
    16261 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    16321 accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgcca cgtgaagtag
    16381 aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccacg cctccggctg
    16441 aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga
    16501 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg
    16561 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    16621 cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt
    16681 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag
    16741 aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtacccc
    16801 ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg
    16861 atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    16921 gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    16981 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtcgtggatg
    17041 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcactgcgc gtgctgtgcc
    17101 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggtgcaatg gccgaatgcc
    17161 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    17221 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    17281 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    17341 cgctgatcga actggatgac tttgccgaat tgcacaaaga ggtctttggt ccggtgctgc
    17401 atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg
    17461 gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg
    17521 gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttggtg
    17581 tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    17641 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc
    17701 aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg
    17761 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    17821 agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct
    17881 ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga
    17941 ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc
    18001 atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag
    18061 cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc
    18121 ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt
    18181 ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg
    18241 tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa acaggtagcc
    18301 ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc
    18361 cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc
    18421 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa
    18481 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    18541 gcagatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc
    18601 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact
    18661 tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac
    18721 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    18781 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc
    18841 tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    18901 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    18961 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    19021 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    19081 gtttagtcca ctttttatca gatattttag ataacgtaat attaacatat taataacatt
    19141 aactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    19201 gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    19261 aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca
    19321 gtacatgccg acctttgaac tgaataaagc cattgtgcag aaagcggagc attaccattt
    19381 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    19441 tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct cgcgcattca
    19501 gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    19561 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    19621 gcccgtgtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    19681 ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgatt
//
LOCUS       DAEEBB010000078        19407 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEEBB010000078 DAEEBB010000000
VERSION     DAEEBB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.078"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..245
                     /locus_tag="KLF07_002545"
     CDS             <1..245
                     /locus_tag="KLF07_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBD9832536.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="KLF07_002546"
     CDS             complement(296..1063)
                     /locus_tag="KLF07_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD9832537.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="KLF07_002547"
     CDS             1259..2167
                     /locus_tag="KLF07_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD9832538.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="KLF07_002548"
     CDS             2164..3426
                     /locus_tag="KLF07_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBD9832539.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="KLF07_002549"
     CDS             3423..4061
                     /locus_tag="KLF07_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBD9832540.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="KLF07_002550"
     CDS             4066..4842
                     /locus_tag="KLF07_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBD9832541.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="KLF07_002551"
     CDS             4931..6295
                     /locus_tag="KLF07_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBD9832542.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="KLF07_002552"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="KLF07_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBD9832543.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="KLF07_002553"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="KLF07_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBD9832544.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="KLF07_002554"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="KLF07_002554"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBD9832545.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="KLF07_002555"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="KLF07_002555"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBD9832546.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="KLF07_002556"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="KLF07_002556"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBD9832547.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="KLF07_002557"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="KLF07_002557"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBD9832548.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="KLF07_002558"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="KLF07_002558"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBD9832549.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="KLF07_002559"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="KLF07_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBD9832550.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="KLF07_002560"
     CDS             complement(12844..13167)
                     /locus_tag="KLF07_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBD9832551.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="KLF07_002561"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="KLF07_002561"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBD9832552.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="KLF07_002562"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="KLF07_002562"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBD9832553.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="KLF07_002563"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="KLF07_002563"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBD9832554.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="KLF07_002564"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="KLF07_002564"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBD9832555.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="KLF07_002565"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="KLF07_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBD9832556.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="KLF07_002566"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="KLF07_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="KLF07_002567"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="KLF07_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBD9832557.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5230 c         4847 g         4738 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAEEBB010000079        18843 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEEBB010000079 DAEEBB010000000
VERSION     DAEEBB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.079"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(253..2124)
                     /gene="ppiD"
                     /locus_tag="KLF07_002568"
     CDS             complement(253..2124)
                     /gene="ppiD"
                     /locus_tag="KLF07_002568"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD9832558.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2316..2588)
                     /gene="hupB"
                     /locus_tag="KLF07_002569"
     CDS             complement(2316..2588)
                     /gene="hupB"
                     /locus_tag="KLF07_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBD9832559.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2797..5151)
                     /gene="lon"
                     /locus_tag="KLF07_002570"
     CDS             complement(2797..5151)
                     /gene="lon"
                     /locus_tag="KLF07_002570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBD9832560.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5339..6613)
                     /gene="clpX"
                     /locus_tag="KLF07_002571"
     CDS             complement(5339..6613)
                     /gene="clpX"
                     /locus_tag="KLF07_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBD9832561.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6739..7362)
                     /gene="clpP"
                     /locus_tag="KLF07_002572"
     CDS             complement(6739..7362)
                     /gene="clpP"
                     /locus_tag="KLF07_002572"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBD9832562.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7608..8906)
                     /gene="tig"
                     /locus_tag="KLF07_002573"
     CDS             complement(7608..8906)
                     /gene="tig"
                     /locus_tag="KLF07_002573"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBD9832563.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9250..9567)
                     /gene="bolA"
                     /locus_tag="KLF07_002574"
     CDS             complement(9250..9567)
                     /gene="bolA"
                     /locus_tag="KLF07_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBD9832564.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9872..10450
                     /locus_tag="KLF07_002575"
     CDS             9872..10450
                     /locus_tag="KLF07_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD9832565.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10494..11969
                     /gene="ampG"
                     /locus_tag="KLF07_002576"
     CDS             10494..11969
                     /gene="ampG"
                     /locus_tag="KLF07_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBD9832566.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12430..13377
                     /gene="cyoA"
                     /locus_tag="KLF07_002577"
     CDS             12430..13377
                     /gene="cyoA"
                     /locus_tag="KLF07_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBD9832567.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13399..15390
                     /gene="cyoB"
                     /locus_tag="KLF07_002578"
     CDS             13399..15390
                     /gene="cyoB"
                     /locus_tag="KLF07_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBD9832568.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15380..15994
                     /locus_tag="KLF07_002579"
     CDS             15380..15994
                     /locus_tag="KLF07_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBD9832569.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15994..16323
                     /locus_tag="KLF07_002580"
     CDS             15994..16323
                     /locus_tag="KLF07_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBD9832570.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16335..17225
                     /gene="cyoE"
                     /locus_tag="KLF07_002581"
     CDS             16335..17225
                     /gene="cyoE"
                     /locus_tag="KLF07_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBD9832571.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17410..17721
                     /locus_tag="KLF07_002582"
     CDS             17410..17721
                     /locus_tag="KLF07_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD9832572.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17705..18247
                     /locus_tag="KLF07_002583"
     CDS             17705..18247
                     /locus_tag="KLF07_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD9832573.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18303..>18843)
                     /locus_tag="KLF07_002584"
     CDS             complement(18303..>18843)
                     /locus_tag="KLF07_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD9832574.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4217 a         4954 c         4579 g         5093 t
ORIGIN      
        1 cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc
       61 cagggacagg gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt
      121 gttaacagtg gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa
      181 atggaaaagg ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag
      241 gcggcttcag gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg
      301 caggttactc atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac
      361 catcgctttt ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag
      421 aaccacatta ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag
      481 tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg
      541 ctcgccaaat ttcagaccgg cagcctgcat agcttccgcc cctttgccgg ctttcaaatc
      601 aaccagcagt ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag
      661 tgccttaact tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct
      721 gatgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccgg
      781 cgcgccgttt tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt
      841 caactcttcc ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc
      901 agcttgctct gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg
      961 ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc
     1021 acgtacttcg tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag
     1081 gaaaccgacc gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt
     1141 tttcagttcg tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc
     1201 agagataata tcagcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag
     1261 ctcatcaagt accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt
     1321 acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc
     1381 gctaaccggt tgctgcatcg ttgcggcatc cagcttgatg taactcacgc ggaattgttc
     1441 cggcgtcatg aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac
     1501 aggctgcttc gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg
     1561 cgcgaccagt gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac
     1621 accgttaatc agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc
     1681 ggtcatcccc atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac
     1741 ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt
     1801 cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg
     1861 acgcagggtt ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg
     1921 ttgctgcatg cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc
     1981 ctggtcattc acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc
     2041 ggtcaatatg aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc
     2101 agccgtgcgt aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc
     2161 tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca
     2221 gcccacaaga cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc
     2281 cttgaacttc gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct
     2341 ttacctgcac ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc
     2401 gggttgcggc cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt
     2461 gctacatcat ccccttcttt cagagattca gttacggaag caataatagc atctaacgca
     2521 cggccagccg cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat
     2581 ttattcactc ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga
     2641 acgcgcagca gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc
     2701 ccgatccgcc atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc
     2761 agccctgttt ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat
     2821 gccagacggt tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg
     2881 aatgtccaga tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa
     2941 cggaattagc actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc
     3001 accgatcggc agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg
     3061 aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg
     3121 accatctttc ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa
     3181 atcagggttg atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat
     3241 agactcctgc atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac
     3301 acatgcggtt tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccggttac
     3361 ctgaccgaca cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta
     3421 gtcatgcagg ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg
     3481 cttaaccgct ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc
     3541 acgggtgtag taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt
     3601 tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt
     3661 ttcatcttcg gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg
     3721 aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc
     3781 caggtagtgg tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga
     3841 ggccggatcg cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg
     3901 gtttttcacg cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta
     3961 agtacggcgg tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac
     4021 atatttacgc ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg
     4081 cggccctacc aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag
     4141 gtactcaagg atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc
     4201 ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat
     4261 ccagtcgata taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag
     4321 cttctgcaac tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat
     4381 tttgcgcttc agggcttcgt tttcgtccgg cgcgtcgtcc atttcaccga gttctttctg
     4441 aatagctttc atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt
     4501 aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc
     4561 catcagatat tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc
     4621 cagtttcagc ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct
     4681 attcagcgac gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa
     4741 ctggctgatt gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc
     4801 cagatactcc gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc
     4861 gcgctgtaac ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa
     4921 tatagaggcc acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc
     4981 ttctttctgc gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg
     5041 gatagatttt tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc
     5101 gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc
     5161 tcttagttta atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta
     5221 acatgtacgt cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa
     5281 attaaagggg agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt
     5341 attcaccaga tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac
     5401 catcaattac cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt
     5461 acatggtatc gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac
     5521 gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc
     5581 cttccagatt aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga
     5641 tctgaatcag agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa
     5701 tgaactcagg gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct
     5761 cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc
     5821 cggtttctac acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa
     5881 acaggatctt agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt
     5941 gcggtggaac agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtacgcctt
     6001 caccggaaac gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt
     6061 catcgatgta gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca
     6121 gcttctgaat gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg
     6181 cgtcggccat ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt
     6241 taccggaacc ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc
     6301 tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt
     6361 tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg
     6421 gtagcgcact gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt
     6481 tacataaatc aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca
     6541 cttcatgctg gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc
     6601 gtttatctgt catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa
     6661 atgccattct caggcgcaag ccgcttacag cgttgtgccg ccctggataa gtatagcggc
     6721 acagttgcgc ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc
     6781 caccgcttca ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa
     6841 tgattgaccc gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc
     6901 acgggcatga atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat
     6961 catcacgcgc gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcagcaagaa
     7021 agcgcccatc gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg
     7081 catggtgtca tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag
     7141 atagatatct ttttctgggt tttccgcttc taggaacagc atctgcgcca caatcaggtt
     7201 cgccatgtgg tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga
     7261 atagatatca aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc
     7321 catatggggt gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa
     7381 attgaaaaaa cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt
     7441 gttatgggta cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac
     7501 cgaaagaggc ttttcgcacg gttccatgca aaattcatga caaaaaaaag cccaccacct
     7561 ggcggtgacg ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg
     7621 ttcatcagct cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct
     7681 tcaacagcct gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg
     7741 ctgtagaact cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg
     7801 cctttcacgc gctcttcgtc agctttcagt tcgttggtgc ggataacttc gcccagcagc
     7861 aggccaacaa ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga
     7921 gcttgttttt cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg
     7981 ctgtcgatca gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc
     8041 tgagacttaa cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact
     8101 tcagcgcgca gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca
     8161 gtcagttccg gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct
     8221 ttacctttca gattttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct
     8281 tcgccagctt tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc
     8341 atcgccagta cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg
     8401 gtgaagtcga tggttacgcg gtcttctgct tcaacggcgc cgtctttttc tttccaggtc
     8461 gcctgctgtt tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg
     8521 atcggttttt caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca
     8581 aactctacag agtaggtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt
     8641 gcgccagccg gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg
     8701 tcacccagaa cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc
     8761 actttgcctt tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt
     8821 ttaacagcgg tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct
     8881 tgagtggttt caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa
     8941 ctcttacccc gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat
     9001 cacattccct gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg
     9061 gcatcacttt tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc
     9121 ccgggcaatt ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg
     9181 cccgcaggcc gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca
     9241 aatgcgtttt tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc
     9301 ctgcaaccct tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt
     9361 agagagttcc tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc
     9421 cgtaaaacga tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt
     9481 gtgacgatag ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt
     9541 ttcttctatc cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat
     9601 ccatcccttt aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt
     9661 aggtttcgtc ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca
     9721 caggcctcgc atcagcgttt cttttaccag ggatgattat ttcgccaaca tgatgaattt
     9781 acatgcctta cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca
     9841 aacacgctta agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt
     9901 tgctctgttt atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac
     9961 gattacactg ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc
    10021 cgatcagcgt accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct
    10081 gaccgcctcc cgagatctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc
    10141 gcgtggttac atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact
    10201 gtatgctgac gtgtcccagg gcaacgtgcg ctacaacatc gcgaccaaag cggatatcgc
    10261 catcatcgct accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa
    10321 cgttgaaggt gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct
    10381 gaccgatacc atcgctgata tgtctcagga caccagcatc cacgaattca tcaagcagaa
    10441 cgcgcgttaa tttctgccct ctggcccggt gcaaaccggg cctgtagacg cccatgtcca
    10501 gtcaatattt acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg
    10561 cttccgggct accgctcgcc ctgacatccg gtaccttaca ggcctggatg acggtcgaga
    10621 atattgatct caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat
    10681 tcctctggtc accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt
    10741 ggctgctcgc cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac
    10801 ctggcaccca actccgctgg atggcggcac tggcggtggt gatcgctttt tgctcggcct
    10861 cccaggatat tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg
    10921 cgggcgcggc aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcttag
    10981 ccctgtggct ggcagataaa tggctgggct ggcagggcat gtactggttg atggcggcgc
    11041 tgttgatccc ttgtattatc gcgacgttgc ttgcaccaga accgaccgac accattcctg
    11101 tgccaaaaac gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata
    11161 atgcctggct tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga
    11221 gcctgacaac cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg
    11281 ttaacaaaac gcttggctta ctggcgacca ttgtgggcgc attgtacggt gggattttga
    11341 tgcagcgcct gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta
    11401 acgctggtta ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg
    11461 tctttttcga aaacctctgt ggcgggatgg gcacatcagc ctttgtcgca ctgttaatga
    11521 cgctgtgtaa taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg
    11581 taggacgagt ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga
    11641 cattctatct attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc
    11701 gccagacgct tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccga
    11761 caggttatgc ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt
    11821 tactgctgtt gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc
    11881 tggaagtcgg ggttttagtc gccctttctg gcgtcgtgct tggtggtttg ctggattatc
    11941 tggctctacg aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac
    12001 tcgccgtaat tattacggct aaataaacat cagtatcgct taataatatt tgttcttttg
    12061 tgcggcttag cgtttggaaa ttattgacgc aatttatgaa ttctattttt tcttacacga
    12121 ttcagctaat gagtctttat tttcccccca cccaattgtc accataatga taattatttg
    12181 ttaaacaatt gttttattca agattggtta taccaattgc ccgtccaaac tccttctgca
    12241 ctcccctttc gttataacgc ccttttgcaa cagcttctta aaatcaactt gatatgtttt
    12301 gcaacatatg taacctgaca gccaaatcca agtaacagga atttaatcat gtttacagta
    12361 atgtaacctc cccgtaaaat gcccacacac tttaaacgcc accagatccc gtggaattga
    12421 ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg gatggttgtc attatttgca
    12481 ggcactgtat tgctcagtgg ctgtaattct gcgctgttag atcccaaagg acagattggt
    12541 ctggagcaac gttcactgat actgacggca tttggcctga tgttgattgt cgttattccc
    12601 gcaatcttga tggctgttgg tttcgcctgg aagtatcgtg cgagcaataa agatgctaag
    12661 tacagcccga actggtcaca ctccaataaa gtggaagctg tggtctggac ggtacctatc
    12721 ttaatcatca tcttccttgc ggtactgacc tggaaaacca ctcacgctct tgagcctagc
    12781 aagccgctgg cacacgacga gaagcccatt accatcgaag tggtttccat ggactggaaa
    12841 tggttcttca tctacccgga acagggcatt gcaaccgtga atgaaatcgc tttcccggcg
    12901 aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt cttcattccg
    12961 cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc
    13021 aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg cttctcaggc
    13081 atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg ggtcgcaaaa
    13141 gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact ggccgcgcct
    13201 agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt tgccgatgtg
    13261 attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga aggtgagcac
    13321 agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc ccattaaagg
    13381 ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc gttccatgaa
    13441 cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct cgttggcctg
    13501 atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc cgtcgaccat
    13561 aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg tggttttgct
    13621 gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggcgaagc gggcttcctg
    13681 ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat cttcttcgtg
    13741 gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt
    13801 gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt tggtgtgatt
    13861 ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca
    13921 ccgctatcgg gaatagagta cagtccggga gtcggtgtcg attactggat atggagtctc
    13981 cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctgaag
    14041 atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc
    14101 gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc
    14161 ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg
    14221 tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat cctgcctgtt
    14281 ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc
    14341 tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtttg gctgcaccac
    14401 ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacaatgatt
    14461 atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc
    14521 atcgtgttcc attctgcgat gctgtggacc atcggtttta tcgtcacttt ctcggtgggc
    14581 gggatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg
    14641 ttcctgattg cgcacttcca taacgttatc atcggcggcg tggtcttcgg ctgcttcgca
    14701 gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaatgaaac ctggggtaaa
    14761 cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct gtatgcgctg
    14821 ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacaccatg
    14881 ctgatgattg cagctagcgg tgcagtactg attgcgctgg gtattctctg cctcgttatt
    14941 cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg cgacccgtgg
    15001 ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt
    15061 gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga aagagaaagg cgaagcgtat
    15121 aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc aggtatcgtc
    15181 attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg
    15241 attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg
    15301 gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt
    15361 actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg cccacgcgca
    15421 cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat
    15481 gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg tgaacggcac
    15541 cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt
    15601 cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa
    15661 caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg gatttatcgg
    15721 gatggaaatc tacgaattcc atcacctgat tgttaacggc atgggtccgg atcgcagcgg
    15781 cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct
    15841 tatctggatg gcggtgctga tggtacaaat cgctcgtcgc ggcctgacca gcactaaccg
    15901 tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt
    15961 gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga tcacagcggc
    16021 gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat cattctgacg
    16081 gtgattccgt tctggatggt gatgacagga gctgcctctc cggccgtaat tctgggaaca
    16141 atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct gcacatgaat
    16201 accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct
    16261 atcctggttg taggctccat ctggattatg tggaacctca actacaacat gatgatgcac
    16321 taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca ggcatcatct
    16381 ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc agcattgatt
    16441 atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt
    16501 ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc
    16561 tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg ctaggctttg
    16621 ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg
    16681 gctttgtggt ttatgtcggc gtttatagcc tgtacatgaa acgccactcg gtctacggca
    16741 cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat tggctactgt gcagtaaccg
    16801 gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc
    16861 actcctatgc catcgccatt ttccgcttta aggattatca ggcggcaaac attccggtat
    16921 tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct
    16981 ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa tatctggtgg
    17041 tcgccgcggc ggtgagcgtc tggtggttag gtatggccct gcgcggttat aaagttgctg
    17101 atgacaggat ctgggcgcgc aagctgttcg gcttctctat catcgccatc acagccctct
    17161 cggtgatgat gtccgttgat tttatggtgc cggactcgca tacgctgctg gctgctgttt
    17221 ggtaacacaa cctctctatt aaaaaggtgc tacggcacct tttttattgt ctgagatcag
    17281 agatacggct aataaccgta tgtcttgaca gagtgagcag atgaacataa attaatcata
    17341 cggttgatag ccgtattatg tatgagatcg actccctacg atttcattca gttaatcaaa
    17401 gaggtcataa tgcctggaaa aacagccact ctacccgatg tcgataaaac gctgaaaaat
    17461 gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca aacttcgtga agctgcgcaa
    17521 gccgttgggg tagatttaag cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt
    17581 gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa
    17641 ctcatcgctg aacctgctca accgacgctc gccctcaagg cgttaatgaa aaggaaaaac
    17701 agcgatggtc gacaagcatg aagagattac tctgcccata gtcctctcct gtaattatca
    17761 gtctgatatt acttatcctg ggcaaaaaca gtttgattgc ggtaaccctg ttatcgataa
    17821 atttgtacgc gcatcgctaa agaaaagtgt gcgtaatagc gactgtgcgg ctaaagcact
    17881 tattgacaga caaagtggtg aactgatcgg catctgtact tttacggcat attcgctgga
    17941 aaaacaacgc gtttctggcg tccttcaggg ttcacaacct tcagaaattg gtgttgtcag
    18001 attagtcatg ttgggggtag cacggaagta tcaaaagcgg ggctttggtc aggacctact
    18061 atgtgatttt tttgaacatg taaaaataat tcaccaggca ttaccaatta aaggggttta
    18121 tcttgatgct gaccctgccg ccattaattt ttatgctcgt ctcggctttg ttcagctttc
    18181 agcgacacca aatgcttttg gtgctgtacc tatgtttttg gcgattcagc atattctcgc
    18241 ggcttagatt attatcgcca cccgccaggg caagttttgc cttagccggg ggctgagcag
    18301 cgttaccact tttgttcaat tgcccccgct ttcagcaact ccacatcctt gccgttaatg
    18361 gttgccgttg cggcaacgat tgtggtcctt acagttgcac aagattttcg ctcaaattcc
    18421 ggtttataaa gaataagaga cgaattactg gtaaatgaac caaataggta atcagtaaag
    18481 ctaatattca gccggtttaa accgaaaggc gcgaagccaa ccgtttggtt aatctcctta
    18541 ccggatgggc gacgaatgct gaatgtcacg cgaagattgg ccacttcgcc taatttattg
    18601 gtggtggcga agaggctatc catgcaggaa aaacctttat ctaaaaaatt atctcgcatc
    18661 ccagaacggg taaccgtaat cacgggtaac gtatcgtcaa ccgtttttct gtcaatcatg
    18721 tcttttcgac aagtagccag aaagtcttcc gggccaccat aaatgtggac acctttatca
    18781 agatattcct tcgctttctt acaatctacc tcaacatact gaccattggc gtacataatg
    18841 ccc
//
LOCUS       DAEEBB010000080        18663 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEEBB010000080 DAEEBB010000000
VERSION     DAEEBB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.080"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            33..395
                     /locus_tag="KLF07_002585"
     CDS             33..395
                     /locus_tag="KLF07_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBD9832575.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            938..1616
                     /locus_tag="KLF07_002586"
                     /pseudo
     CDS             938..1616
                     /locus_tag="KLF07_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            1660..6510
                     /locus_tag="KLF07_002587"
     CDS             1660..6510
                     /locus_tag="KLF07_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HBD9832576.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6878..8533
                     /gene="lldP"
                     /locus_tag="KLF07_002588"
     CDS             6878..8533
                     /gene="lldP"
                     /locus_tag="KLF07_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBD9832577.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            8533..9309
                     /gene="lldR"
                     /locus_tag="KLF07_002589"
     CDS             8533..9309
                     /gene="lldR"
                     /locus_tag="KLF07_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBD9832578.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            9306..10496
                     /gene="lldD"
                     /locus_tag="KLF07_002590"
     CDS             9306..10496
                     /gene="lldD"
                     /locus_tag="KLF07_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBD9832579.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10692..11165
                     /gene="trmL"
                     /locus_tag="KLF07_002591"
     CDS             10692..11165
                     /gene="trmL"
                     /locus_tag="KLF07_002591"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBD9832580.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(11218..12039)
                     /gene="cysE"
                     /locus_tag="KLF07_002592"
     CDS             complement(11218..12039)
                     /gene="cysE"
                     /locus_tag="KLF07_002592"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBD9832581.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(12119..13138)
                     /gene="gpsA"
                     /locus_tag="KLF07_002593"
     CDS             complement(12119..13138)
                     /gene="gpsA"
                     /locus_tag="KLF07_002593"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBD9832582.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(13138..13605)
                     /gene="secB"
                     /locus_tag="KLF07_002594"
     CDS             complement(13138..13605)
                     /gene="secB"
                     /locus_tag="KLF07_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBD9832583.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13668..13919)
                     /gene="grxC"
                     /locus_tag="KLF07_002595"
     CDS             complement(13668..13919)
                     /gene="grxC"
                     /locus_tag="KLF07_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBD9832584.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(14061..14492)
                     /locus_tag="KLF07_002596"
     CDS             complement(14061..14492)
                     /locus_tag="KLF07_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBD9832585.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14737..16281
                     /gene="gpmM"
                     /locus_tag="KLF07_002597"
                     /gene_synonym="pgmI"
     CDS             14737..16281
                     /gene="gpmM"
                     /locus_tag="KLF07_002597"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBD9832586.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            16291..17574
                     /gene="envC"
                     /locus_tag="KLF07_002598"
     CDS             16291..17574
                     /gene="envC"
                     /locus_tag="KLF07_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBD9832587.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            17578..18537
                     /locus_tag="KLF07_002599"
     CDS             17578..18537
                     /locus_tag="KLF07_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBD9832588.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         4736 a         4796 c         4830 g         4301 t
ORIGIN      
        1 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
       61 aacgtctcag cgatcgtctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
      121 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
      181 ctcgcctgcg tgaagttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
      241 tgccgtttca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
      301 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct gggccgtgaa atgggcctgc
      361 aggagatgac tggattctct aagactgcgt tttaagcgcc tcagacgact ggttcgatca
      421 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
      481 ctaattacca aacaattcac tccctttacg tcgatttacc gctggttgag attctgaaaa
      541 tgcattttta ccgaaatagc cacctataaa caataaaaat atatagaaga tatcatttat
      601 taattaacaa gcagttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
      661 attcatacaa taaattatct gtaaattttt tcccagacat ggtttctttc atatttgaca
      721 tttattaata atcatcatta gaatccacga aaataatata ttcatacaaa cacagacatc
      781 ttttgaaacg aatgatacat ttttatgatg aatgcttaaa acaatattta atcgacacac
      841 atcactgtca ttaatgtaaa gcaacataga tcaccaataa tacaatggac aactctaccg
      901 gcattgatcg ctggtcggag gaaataagga tttttacatg aacttgaaga agattttttt
      961 cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca ttgaagaagg
     1021 caagccagaa agtagtttat tgaaacaaca agaagaacat taaaattgat ctacttgaaa
     1081 aacaacaagc acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa
     1141 taagcagtac aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt
     1201 ttatttttac cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg
     1261 tggctttagc gggtcgagtg ttatccattg acgccacaga gaatggcagt gttattcata
     1321 tttcactggt caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg
     1381 gtggtgaaaa acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca
     1441 catcaatgaa atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc
     1501 tgaagggtgt atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata
     1561 ttcagttcga caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc
     1621 agttagtacg cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt
     1681 atctggaacc ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc
     1741 aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg
     1801 tcgtttgggg cattggcgaa tgccgggaat gacaacggcc agggtgttga ttacggtagt
     1861 ggatcagctg gcgacagctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg
     1921 aacaccagtg gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc
     1981 tctgccatcg ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt
     2041 gcaatatcag aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa
     2101 tactcaatgg ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg
     2161 gactcttcca aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact
     2221 gagattatga gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg
     2281 ctgggagcaa gtagcgtcgc atctgaagaa aacgcaattg ccctggggcg tagcagtgta
     2341 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
     2401 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
     2461 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
     2521 actatcgcat taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggccag
     2581 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
     2641 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
     2701 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
     2761 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
     2821 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
     2881 attagcgact cggtagcaaa aaggcttgga ggaggggctg cagtagatgt tgatgacggt
     2941 actgttacag caccaaccta caatttaaaa aatggtagca aaaataacgt aggggctgcg
     3001 ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct
     3061 gctcatggta ctagtagccc aactgccagc gtaatcaccg atgttgcgga tggcacgatt
     3121 tcagcctcca gtaaggatgc ggttaacggt tcccaactga aagctaccaa tgacgatgtc
     3181 gaagccaaca ccgccaatat cgctactaat accagcaaca ttgccacgaa tacagcaagt
     3241 attgccacca ataccaccaa tatcaccaac ctgacggatt ccgttggtga ccttcaggct
     3301 gatgccctgc tctggaacga aactaaaaag gcattcagtg cagctcacgg ccaggatacc
     3361 accagcaaaa tcaccaacgt taaagatgcc gacctgacgg ctgacagcac tgatgctgtt
     3421 aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc
     3481 aataacactt ccaatattgc cactaacacc accaacatct ctaatctgac tgagacggtg
     3541 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct
     3601 catggcaccg agaccaccag caaaatcacc aacgttaaag atggcgacct gacgactggc
     3661 agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat
     3721 accaccaata tcgccactaa caccaccaac atctctaatc tgactgagac ggtgactaat
     3781 cttggtgagg atgcgctgaa atgggataag gacaatggtg tcttcactgc agctcatggc
     3841 aacaataccg ccagcaaaat caccaatatc ctggacggca cagtcactgc aaccagttcc
     3901 gatgccatta acggtagcca gctttatgac ttaagcagca atatcgccac ctacttcggc
     3961 ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc caacctacaa aatcggtgaa
     4021 acaaattatt ataacgtcgg cgatgcactg gctgcgatta actcctcatt tagcacgtct
     4081 ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa acacggtact
     4141 aatggtgacg caagcgtgat cactgatgtc gcagatggtg aaatttcaga ctccagttct
     4201 gacgcagtaa acggctcaca actccacggc gtgagcagtt atgttgttga tgcgctgggg
     4261 ggtggtgccg aagtcaatgc agacggcacc atcactgcgc cgacgtacac cattgctaat
     4321 gctgattacg ataatgtcgg tgatgccctg aatgctatcg ataccactct tgacgacgct
     4381 ctgctctggg atgcggacgc cggtgaaaat ggtgcattta gcgccgctca cggaaaagat
     4441 aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc cagcagcgac
     4501 gcgattaacg gctcacaact ctataccacc aataagtaca tcgctgatgc gctgggtggt
     4561 gacgcagaag tcaacgctga cggcaccatc accgcaccga cttacaccat tgcgaacgcc
     4621 gagtacaaca acgtcggtga cgccctggat gcgcttgatg ataacgccct gctgtgggat
     4681 gagactgcca atggcggtgc tggagcctac aatgccagcc atgacggtaa agccagcatc
     4741 atcactaatg tcgctaatgg cagtattagt gaggacagta ccgatgcagt gaacggttct
     4801 cagttgaatg cgacgaatat gatgattgag cagaacaccc aaattatcaa tcagctcgct
     4861 ggtaacaccg acgcaaccta tatcgaagaa aatggcgctg gtatcaacta cgttcgtact
     4921 aacgacaacg atttagcctt taacgatgca agcgcctctg gtgtcggcgc tacagctgta
     4981 ggttataacg ctgtcgcgtc tggtgccagc agcgtagcca ttggtcagaa cagcagcagc
     5041 accgttgata ccggtattgc gctgggtagc agctccgttt ccagccgtgt gattgccaaa
     5101 ggttctcgtg acactagcgt aacggaaaat ggcgtggtga ttggttatga caccactgac
     5161 ggcgaactgc taggtgcatt gtcaattggt gatgacggta aataccgcca aatcatcaac
     5221 gtagctgatg gttcagaagc ccatgacgcc gttacggttc gccagttgca gaacgctatt
     5281 ggagcggtcg ccactacgcc aaccaagtac tttcacgcca actcaacggc agaagactca
     5341 ctggccgttg gtgaagactc gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca
     5401 ggtattggta tcggtttgaa cactctggtt ctagctgatg cgatcaacgg cattgctatc
     5461 ggcagtaacg cacgcgcaaa ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc
     5521 acccgtggtg cacagactgg ctacaccgcc tacaacatgg acgcaccgca gaactctgta
     5581 ggtgagtttt ctgtcggtag tgaagacggt caacgtcaga tcacaaacgt cgcagctggt
     5641 tcagcggata ccgatgcggt taacgtgggt cagttgaaag tcactgatga gcgcgtagcg
     5701 caaaataccc agagcattac taacctgaac aatcaggtca ctaatctgga tactcgcgtt
     5761 actaatatcg aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag
     5821 accaacaccg atggcgtaga tgccaacgcc cagggtaaag atagcgttgc tattggttct
     5881 ggttccattg ctgccgctga caacagcgtc gcactgggta ccggttccgt tgcagaggaa
     5941 gaaaatacaa tctctgtagg ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc
     6001 ggtgttaatg ccaccgatgc ggttaacgtg tcgcagctga aatcttctga agcaggcggc
     6061 gtgcgctacg acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggt
     6121 ggcaacggca gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg
     6181 gtgaactacg cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcgg
     6241 atggttgaga tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct
     6301 tctgcaatgg caatgaccgg tctgccgcag gcttatacac cgggtgccag catggcttct
     6361 attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc
     6421 gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc
     6481 gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca
     6541 aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga taaggcgccc cgggtcaaca
     6601 atctaagggc aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca
     6661 agaattggcc ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa
     6721 actcacatca atattgccaa tacataacat ttagttaacc attcattgtc attatcccta
     6781 cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac
     6841 tcattacacg atgtgcgtgg actccaggag acctgcaatg aatctctggc aacaaaacta
     6901 cgatcccgcc gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt
     6961 cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc
     7021 aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc
     7081 ggtggtttat ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt
     7141 gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct
     7201 ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt
     7261 ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg
     7321 cctgggtttt aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt
     7381 ggcatttggc gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag
     7441 ctttgagatt ggccagatgg tggggcggca gctaccgttt atgaccatta tcgtgctgtt
     7501 ctggatcatg gcgattatgg acggctggcg cggtatcaag gagacgtggc ctgcggtcgt
     7561 ggttgcgggc ggctcgtttg ccatcgctca gtaccttagc tctaacttca ttgggccgga
     7621 gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg
     7681 ctggcagcca gtgcgcgtat tccgttttgg tgatttgggg gcgtcaaaga ttgatatgac
     7741 gctggcccac accggttaca ctgcgggtca ggtgttacgt gcctggacac cgttcctgtt
     7801 cctgacagct accgtaacac tgtggagcat cccgccgttt aaagccctgt tcgcatcggg
     7861 tggtgcgctg tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc
     7921 ccgtatgcca ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg
     7981 gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa
     8041 gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc
     8101 catctactcc atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc
     8161 atcaacactg gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt
     8221 cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc
     8281 cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc
     8341 caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg
     8401 tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag
     8461 cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg
     8521 gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg
     8581 cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa
     8641 ctggcgatgc aactcggcgt atcacgtaat tcactgcgcg aggcgctggc aaaactggtg
     8701 agcgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac
     8761 acatggtcgg agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat
     8821 tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg
     8881 gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta
     8941 agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa
     9001 gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc
     9061 tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc
     9121 gaacaacatc aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa
     9181 gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag
     9241 gctcgccacg cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa
     9301 aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc
     9361 cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg
     9421 tggaagattt gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tccgacttaa
     9481 gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg
     9541 gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc
     9601 atggcattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag
     9661 ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta
     9721 acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc
     9781 cgacaccggg cgcacgctac cgtgatgcgc attcaggtat gagcggccca aatgcggcaa
     9841 tgcgccgcta cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg
     9901 gtcgtccaca tgatttaggt aatatctcag cttatctcgg caaaccgacc ggactggaag
     9961 attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt
    10021 ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg
    10081 cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc
    10141 agctggacgg tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag
    10201 gtgatatcgc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga
    10261 ttgcgctcgg tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag
    10321 cgggccaggc gggtgtagct aacctgctaa atctgatcga aaaagagatg aaagtggcga
    10381 tgacgctgac tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaggggc
    10441 tgggtaaaga gttgcctacg gcactggctc cgatggcgaa agggaatgcg gcatagtcgt
    10501 ttgcgccccc tcaccctaac cctctccctc agggagaggg gaccgttcgg cgctgtatgt
    10561 actccctcac tctgaaacga caccgcactc tttttttctc cctcgcccct ccggggaggg
    10621 ccggggtgag gggaaaaggc cgcacggttc accaattttc tgctatcctg cccccgcact
    10681 aagggggcag tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca
    10741 acatcatccg tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccgatgggat
    10801 ttgcctggga cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg
    10861 ttacgcgtca tcatgactat cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg
    10921 ccctcaccac gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat ggcgattatc
    10981 tgatgttcgg cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg
    11041 aacaaaaaat tcgcattccg atggtgccgg acagccgcag catgaatctg tccaatgcgg
    11101 tgtcggtagt ggtgtatgaa gcctggcggc agctgggata tccgggagcg ttgttgagag
    11161 attagttact gtatgccgga tagatgatta catcgcatcc ggcacgatca caggacatta
    11221 gatcccatcc ccatactcaa atgtatggtt aataccgttg aaatgctggt ccatatccat
    11281 tgatggctta tcgctgtctg gtttaccgac aatacgagcc ggaacgccag cggcggttgt
    11341 atgcggcggc accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac
    11401 ttcaatattg ccgaggattt tcgcgcccgc gccaatcatc acaccttcac gaattttcgg
    11461 gtgacggtca ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac
    11521 gtcgttttca attaccgccg tttcacccac aacgatgccc gtggcgtggt cgagcataat
    11581 gccacggcca atttttgctg ccgggtgaat atcgacctgg aacgtcacag aaacctggtt
    11641 ttgcagaaag attgccaatg cgcgacgccc ctgattccac aaccagtgac cgatgcgata
    11701 ggcctgcaag gcatgaaaac ccttcaggta taacaacggg gttgagtatt tatcgactgc
    11761 cgggtcgcgg gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc
    11821 agcggcgtag gcttcttcca ccacttcacg gatagcaata gcaggcataa ttggcgatga
    11881 cagcttgttc gccagcatgt agctcagtgc actaccaagg ttttcgtgct tgagtagcgt
    11941 cgcgtggtaa aaactggcca gcattggctc acagtccgcc agcgttctgg cttcggcttt
    12001 aatattgttc cagacaattt ccagttcttc acacgacatt gcttactcca cacgatgaga
    12061 taatgaccgg cccgttctgc gcgggccggg tcatagcggt aacaaaggtt ccctggggtt
    12121 agtggctgct gcgctcgtcc ttgcgtgcac gacctaataa cgtcaatgct gcctcgcgcg
    12181 cgttttttcc gcaatataat acttgataaa tttcctcggt tattggcatt tctacgccga
    12241 agcgatgcgc cagttcgcgg acttctttcg tattgcggta gccttccacc acctgaccaa
    12301 tcttctcctg cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt
    12361 tacgcgactg gttgtcggta caggtaagca ccagatcgcc cagccccgcc atgcccataa
    12421 aggtggcagg atcggcaccc agcgccgcgc caagacgcga catttcagcc agcccacggg
    12481 tgatcagtgc cgtacgcgca ttcgcaccaa aaccgatacc gtcggacatc cccgcgccaa
    12541 tggcaatgac gtttttcacc gcaccgccaa gctgcacacc gatgaaatcc gggttgctgt
    12601 aaacgcggaa acttttgcca cagtgcagca attgttggag atcatcggca aaggtctggt
    12661 cggtagaggc caacgaaata gccgtcggta aacctgccgc cagttctttc gcaaacgttg
    12721 ggccagagat aaccgccagc ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta
    12781 acagacgtcc ggtttccgct tccagccctt tggtcgccca caccagacgc gcatcaggac
    12841 gcatcagcgg tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga
    12901 gaatattacg gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg
    12961 gaaaaggcac atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat
    13021 gttcagggtc atggccccag aggacaacct cgtggccatt tcttgccagg gtaatggcaa
    13081 gagcggtgcc gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca
    13141 ggcatcctga tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag
    13201 cgcatcgaag ttaatcggcg caaggttcag ttgcgggaat gtaccgcggg ataccacgct
    13261 ggtgatgcac tcacgagcat acgggaacag aatgttcggg cagtatgcgc ccaggcaatg
    13321 tgccatctgg gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca
    13381 caggaacgcg gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta
    13441 tacgtcatct gccagttggg aagatgccgt atccagatca agtttaactt ctggttgcca
    13501 atctttctgg aaaacgtgcg gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat
    13561 acgttggatc tggaaagtca tttcagtgtt gttttgttct gacatgtgta gaaaaccctt
    13621 aagtgttgtc cttaaatact gcgcaatgta atgccaacgt tcacacgtta tttcagcagg
    13681 ggatccagtc caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt
    13741 gcgtcaataa aaatctgggg aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc
    13801 ttggcggcat tgccatcgat cggcaactcc tgaaaactca cgcccttgct gctcagcagt
    13861 gcttttgcac gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata
    13921 atttagctcc tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt
    13981 gtccccagaa gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat
    14041 catgaaagac gacaggtaaa ttatttgccg cgcaccagag gcaagttttc gccagcccag
    14101 ccagcaacgc cctctttcag cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt
    14161 gcaggctcct ggcactgcat gccagaaccg tctaccacga taaccggttt gtctttgtgc
    14221 ttctcaagct caccaacatt gttggctttg atttcgctcg gcaacagatt gatagaacct
    14281 gcgatatggc ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct
    14341 ttgttgatca gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc
    14401 ttaaacgtag tcacaagaac cgccaccagt aacgcgatcc aggcgatact cagtatggga
    14461 tgacggccaa caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat
    14521 taaaaaaacc aggaaaggag tatacctgcg cggtgcggca aatacagcca gcgcgttaac
    14581 tggaatgcaa ttttgcgggg cacgacgaaa aaaaagcgca aatctggcat cggcttgccg
    14641 cgagcggtcg tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt
    14701 acgcaaattt tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg
    14761 gtactggtga ttctggatgg atatggctat cgtgaagaac agcaggataa cgccattttt
    14821 agtgctaaaa ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac
    14881 gcttccggtc tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggccac
    14941 gttaacctgg gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc
    15001 aaagatcgcg ctttctttgc taatccagtg ctgactggcg cggtagataa agcgaaaaac
    15061 gcaggtaaag cagtacacat tatgggtctg ctctccgcag gcggcgtaca cagccacgaa
    15121 gatcacatca tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg
    15181 cacgcattcc ttgacggtcg cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa
    15241 tttgaagaga aatttgctgc gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac
    15301 tacgcgatgg accgcgataa ccgttgggat cgcgttgaaa aagcctatga cctgctgact
    15361 ctggcgcagg gtgagtttca ggccgatact gccgttgctg gtttgcaggc agcttatgct
    15421 cgcgacgaaa acgacgagtt cgtgaaagcg accgttattc gtgcagaagg tcagccagat
    15481 gcggcaatgg aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt
    15541 gaaatcactc gtgcattcgt gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt
    15601 aacgtcgatt tcgtgatgct gaccgaatac gccgctgaca tcaaaactgc ggttgcttac
    15661 ccacccgctt ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag
    15721 ttgcgtattt ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta
    15781 gaagagtcgt tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac
    15841 gatctgcaac cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggccatcaag
    15901 agcggcaaat acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc
    15961 ggggtgatgg aagcggcggt taaagcggtt gaagcactgg atcactgcgt ggaagaagtc
    16021 gcgaaagcgg ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct
    16081 gaacagatgc gcgatccggc aacgggtcag gcacacacgg cacacaccaa cctgccagtt
    16141 ccgctgattt acgttggtga taagaacgtg aaagcggttg caggcggcaa actttctgac
    16201 atcgcgccga ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag
    16261 ccgctgttca tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca
    16321 cgggccgtga aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc
    16381 gctggcgtat tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct
    16441 attcaggccg atatcgccac aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca
    16501 agcctgctcg cacaattgaa aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg
    16561 cgcgaaacgc aaaacacgct caatcaactc aataaacaga ttgatgagat gaacgcgtcg
    16621 attgccaaac tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactggat
    16681 gccgcattcc gtcagggcga gcataccggt attcagctaa ttctcagcgg tgaagaaagc
    16741 cagcgtggac agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc
    16801 attgcccagt tgaagcaaac gcgtgaagaa gtcgccatgc agcgtgctga actggaagag
    16861 aaacaaagcg agcaacaaac gctgttatat gaacagcgcg cccaacaggc gaaactgact
    16921 caggctctga acgagcgtaa aaagacgctg gcggggctgg aatcttccat ccagcaaggt
    16981 cagcagcagc taagtgaact gcgtgccaac gaatcccgtc tgcgtaacag tattgcccgt
    17041 gcggaagccg cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggctca ggcagttcgc
    17101 gacaggcaga aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa
    17161 tcgctgatgt cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccagtt
    17221 cgtggcccta cgctgcatcg ctatggcgag cagctacagg gtgaattacg ctggaaagga
    17281 atggttatcg gtgcctctga aggtactgaa gttaaagcaa ttgccgacgg tcgggtgatt
    17341 ctggctgact ggctgcaagg ctacggtctg gtggtggtgg ttgagcacgg taaaggcgat
    17401 atgagtcttt acggctataa tcaaagcgcg ctggtgagcg ttggttcgca ggttcgtgcg
    17461 ggccagccaa ttgcactggt gggcagcagt ggcggtcagg gcagaccttc actctatttc
    17521 gaaattcgcc gccagggtca ggccgtcaat ccacagccgt ggttgggaag ataagttttg
    17581 tttccatttc gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccctgta
    17641 attgctggca aacttgccat cgtcattgat gattttgggt atcgcccgaa caacgaaaac
    17701 caggtgctgg cgatgccttc cgctatctcc gtcgctgtat tacccgattc accgcacgcc
    17761 agagagatgg cgaccaaagc gcataacagc ggtcacgaag tgttgattca tctcccgatg
    17821 gcaccgttga gtaaacagcc gctggagaaa aatacgctgc gcccggagat gagcagcgac
    17881 gaaattgagc gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaat
    17941 aaccacatgg gcagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggcg
    18001 ctggagcgtt ataatcttta cttcctcgac agcgtaacca ttggtaatac ccaggcgatg
    18061 cgcgccgcgc aaggcactgg cgtgaaggtg atcaaacgga aggtgttcct cgacgattca
    18121 cagaacgaag cggacatccg tgtgcaattc aatcgcgcaa ttgacctagc gcgtcgcaac
    18181 ggatcaacca ttgccattgg gcatcctcac ccttcaacgg tacgcgtgtt gcaacagatg
    18241 gtttataacc tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg
    18301 caggttgata cgtctacacc gccgaaaaac actgtgcccg acacaccgcg taatccgttc
    18361 cgtggcgtga agctgtgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc
    18421 tttgaagtat taagcgaaag catcagccag agcacgctga tcgtttactt ccagcatcag
    18481 tggcaaggct ggggcaaaca gcctgaagcg gcgaagctta acgctagcgt aaattaaagg
    18541 cgctacgcgt aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag
    18601 actacagtaa cgcctgataa cccaccttta cgctgcgcac attggtaatc aggcgcttat
    18661 aca
//
LOCUS       DAEEBB010000081        18523 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEEBB010000081 DAEEBB010000000
VERSION     DAEEBB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.081"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1710
                     /locus_tag="KLF07_002600"
     CDS             <1..1710
                     /locus_tag="KLF07_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD9832589.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="KLF07_002601"
     CDS             1711..3360
                     /locus_tag="KLF07_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBD9832590.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="KLF07_002602"
     CDS             3365..4141
                     /locus_tag="KLF07_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBD9832591.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="KLF07_002603"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="KLF07_002603"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HBD9832592.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="KLF07_002604"
     CDS             complement(5430..6752)
                     /locus_tag="KLF07_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBD9832593.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="KLF07_002605"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="KLF07_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HBD9832594.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGTVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="KLF07_002606"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="KLF07_002606"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBD9832595.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="KLF07_002607"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="KLF07_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBD9832596.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="KLF07_002608"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="KLF07_002608"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBD9832597.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="KLF07_002609"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="KLF07_002609"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBD9832598.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="KLF07_002610"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="KLF07_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBD9832599.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="KLF07_002611"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="KLF07_002611"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBD9832600.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4525 a         4536 c         4866 g         4596 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaacccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgt gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAEEBB010000082        18513 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEEBB010000082 DAEEBB010000000
VERSION     DAEEBB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.082"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="KLF07_002612"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="KLF07_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBD9832601.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="KLF07_002613"
     CDS             735..1040
                     /locus_tag="KLF07_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832602.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="KLF07_002614"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="KLF07_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBD9832603.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="KLF07_002615"
     CDS             1788..2534
                     /locus_tag="KLF07_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD9832604.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="KLF07_002616"
     CDS             2534..4630
                     /locus_tag="KLF07_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD9832605.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="KLF07_002617"
     CDS             4676..5815
                     /locus_tag="KLF07_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBD9832606.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="KLF07_002618"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="KLF07_002618"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBD9832607.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="KLF07_002619"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="KLF07_002619"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBD9832608.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="KLF07_002620"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="KLF07_002620"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBD9832609.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="KLF07_002621"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="KLF07_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBD9832610.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="KLF07_002622"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="KLF07_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD9832611.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="KLF07_002623"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="KLF07_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBD9832612.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="KLF07_002624"
     CDS             complement(12873..13730)
                     /locus_tag="KLF07_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBD9832613.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="KLF07_002625"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="KLF07_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBD9832614.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="KLF07_002626"
     CDS             complement(14122..14709)
                     /locus_tag="KLF07_002626"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBD9832615.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="KLF07_002627"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="KLF07_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="KLF07_002628"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="KLF07_002628"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBD9832616.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="KLF07_002629"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="KLF07_002629"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBD9832617.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4545 a         4912 c         4781 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gaatcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAEEBB010000083        18214 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEEBB010000083 DAEEBB010000000
VERSION     DAEEBB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.083"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            15..1091
                     /locus_tag="KLF07_002630"
     CDS             15..1091
                     /locus_tag="KLF07_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBD9832618.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="KLF07_002631"
     CDS             complement(1484..1825)
                     /locus_tag="KLF07_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBD9832619.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="KLF07_002632"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="KLF07_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBD9832620.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="KLF07_002633"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="KLF07_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBD9832621.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="KLF07_002634"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="KLF07_002634"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBD9832622.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="KLF07_002635"
     CDS             3559..4215
                     /locus_tag="KLF07_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBD9832623.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="KLF07_002636"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="KLF07_002636"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBD9832624.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="KLF07_002637"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="KLF07_002637"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBD9832625.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="KLF07_002638"
     CDS             complement(7167..7826)
                     /locus_tag="KLF07_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBD9832626.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="KLF07_002639"
     CDS             complement(8153..8509)
                     /locus_tag="KLF07_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBD9832627.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="KLF07_002640"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="KLF07_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBD9832628.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="KLF07_002641"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="KLF07_002641"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBD9832629.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="KLF07_002642"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="KLF07_002642"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBD9832630.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="KLF07_002643"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="KLF07_002643"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBD9832631.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="KLF07_002644"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="KLF07_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBD9832632.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="KLF07_002645"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="KLF07_002645"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBD9832633.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="KLF07_002646"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="KLF07_002646"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBD9832634.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="KLF07_002647"
     CDS             complement(17270..18112)
                     /locus_tag="KLF07_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBD9832635.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4576 c         4391 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgca
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAEEBB010000084        18159 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEEBB010000084 DAEEBB010000000
VERSION     DAEEBB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.084"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="KLF07_002648"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="KLF07_002648"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBD9832636.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="KLF07_002649"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="KLF07_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBD9832637.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="KLF07_002650"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="KLF07_002650"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBD9832638.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="KLF07_002651"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="KLF07_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBD9832639.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3342..3545)
                     /locus_tag="KLF07_002652"
     CDS             complement(3342..3545)
                     /locus_tag="KLF07_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832640.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3623..4717
                     /gene="ddlA"
                     /locus_tag="KLF07_002653"
     CDS             3623..4717
                     /gene="ddlA"
                     /locus_tag="KLF07_002653"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD9832641.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4741..4953)
                     /locus_tag="KLF07_002654"
     CDS             complement(4741..4953)
                     /locus_tag="KLF07_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBD9832642.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5213..5521
                     /locus_tag="KLF07_002655"
     CDS             5213..5521
                     /locus_tag="KLF07_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBD9832643.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="KLF07_002656"
     CDS             complement(5580..6674)
                     /gene="yaiW"
                     /locus_tag="KLF07_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBD9832644.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="KLF07_002657"
     CDS             complement(6687..7907)
                     /gene="sbmA"
                     /locus_tag="KLF07_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBD9832645.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8259..9416
                     /gene="ampH"
                     /locus_tag="KLF07_002658"
     CDS             8259..9416
                     /gene="ampH"
                     /locus_tag="KLF07_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBD9832646.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="KLF07_002659"
     CDS             complement(9417..10040)
                     /gene="iprA"
                     /locus_tag="KLF07_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBD9832647.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10128..13016)
                     /locus_tag="KLF07_002660"
     CDS             complement(10128..13016)
                     /locus_tag="KLF07_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD9832648.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13542..14516
                     /gene="hemB"
                     /locus_tag="KLF07_002661"
     CDS             13542..14516
                     /gene="hemB"
                     /locus_tag="KLF07_002661"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBD9832649.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14622..15473)
                     /gene="tauD"
                     /locus_tag="KLF07_002662"
     CDS             complement(14622..15473)
                     /gene="tauD"
                     /locus_tag="KLF07_002662"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBD9832650.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15470..16297)
                     /gene="tauC"
                     /locus_tag="KLF07_002663"
     CDS             complement(15470..16297)
                     /gene="tauC"
                     /locus_tag="KLF07_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBD9832651.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16294..17061)
                     /gene="tauB"
                     /locus_tag="KLF07_002664"
     CDS             complement(16294..17061)
                     /gene="tauB"
                     /locus_tag="KLF07_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBD9832652.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17074..18036)
                     /gene="tauA"
                     /locus_tag="KLF07_002665"
     CDS             complement(17074..18036)
                     /gene="tauA"
                     /locus_tag="KLF07_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9832653.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4526 a         4722 c         4390 g         4521 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt attataaata
     3361 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
     3421 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
     3481 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
     3541 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
     3601 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
     3661 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
     3721 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
     3781 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
     3841 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
     3901 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
     3961 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
     4021 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
     4081 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
     4141 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
     4201 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
     4261 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
     4321 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     4381 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     4441 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     4501 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     4561 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     4621 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctgaagcg
     4681 tcacgctgcg gataacgcat tgaaagccac aatgtaatat tttaacgccc gataatattc
     4741 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     4801 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     4861 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     4921 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     4981 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     5041 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     5101 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     5161 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     5221 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     5281 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     5341 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     5401 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     5461 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     5521 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     5581 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     5641 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     5701 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     5761 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     5821 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     5881 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     5941 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     6001 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     6061 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     6121 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     6181 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     6241 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     6301 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     6361 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     6421 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     6481 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     6541 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     6601 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     6661 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     6721 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     6781 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     6841 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     6901 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     6961 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     7021 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     7081 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     7141 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     7201 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     7261 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     7321 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     7381 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     7441 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     7501 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcagatca
     7561 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     7621 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     7681 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     7741 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     7801 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     7861 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     7921 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     7981 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     8041 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     8101 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     8161 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     8221 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     8281 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     8341 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     8401 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     8461 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     8521 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     8581 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     8641 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     8701 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     8761 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     8821 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     8881 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     8941 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     9001 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     9061 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     9121 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     9181 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     9241 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     9301 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     9361 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     9421 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     9481 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     9541 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     9601 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     9661 tccagaattc tattttgcca agctaaccag taaaaagcgt ctcgccagag ttgattttgt
     9721 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     9781 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     9841 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     9901 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     9961 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
    10021 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
    10081 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
    10141 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
    10201 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
    10261 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
    10321 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
    10381 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
    10441 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
    10501 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
    10561 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
    10621 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
    10681 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
    10741 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
    10801 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
    10861 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
    10921 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
    10981 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
    11041 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
    11101 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
    11161 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
    11221 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
    11281 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
    11341 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
    11401 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
    11461 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
    11521 gtgtttacat ccagcacacc accgttagta acggtaatgg tattggcata cagcggtgca
    11581 tatgcccaag ctgaagcagt atcttcaccg aagttaacgg ttgagaaggt atcaatggtc
    11641 aggtgatcgg tgttaacttc accgttaact tccattgaag aaccaccgtt cagagtgatg
    11701 gtgtcagaag tcagagaaga atctgcactc acgttaacct gagaaccgct gttgatcgcc
    11761 agggtatcga tcagggaagt tttcgtggtt tcccaatcag aaccgttgtt cagaacaaca
    11821 tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac gattccagcc atcttcagag
    11881 taagaagcgt acgggttcag gctgtttggt gcaacgtcgt agaaggcttc gtcagcaaca
    11941 acaacgtcat aatcaatatt cgctgaacca acccatttgg tgttgttcag agtcaggttc
    12001 aggtgatcga cgttctgatc gtcagttgcc caaccgccat gagtgtaagc agtggtagag
    12061 ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc aggtactgtc aaatacaacg
    12121 tcaccaagca actgagagtt gttgaacgta acgttgttga ccatacgata atcagaagtg
    12181 tcatcatcga tgaaggctaa cgcaacatca tcagcaggag atgcgccatt gccgtagtca
    12241 cttggttcaa cagaattgcc gaaatgacca cgatctgatt gttcgtcttc agtccatgaa
    12301 ccagaagtta cagtagagtc attaacaacg atgttgttgt cccactgatg accttcggta
    12361 ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa tacctgcaac atgggagttg
    12421 ttgctgatgt tgatgttact ttcaacatcc agagtcacag cggtaccaag accagcggtt
    12481 accaaacgat cataatctgt cttagatgtt ttaccgtctg catccagata agtatcggtg
    12541 aagtagaagg cgtcaaaatc atcgtcaata gtagaattag cgatgttcag agtgaagatg
    12601 tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt tggtccagac gtagtcgcca
    12661 taaccaacct ggtaagatgt aaccatacca tgaacaattg agttggagat gttcagtgta
    12721 ttaccggtat ctgtgccata atcgtggttc agataataag tagaaattac accatttaca
    12781 gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg cgtggtcata tacataacca
    12841 ccgtaagtca gataatcgtg atcaatttta gtgctgtcac cccaattgta gatggtggct
    12901 gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat tagcctgaga ggtgatagcc
    12961 agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga gtgcatttgt
    13021 catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata gaaaacatat
    13081 tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta ttctttcccc
    13141 taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag
    13201 gacgatattt caacatgttg cagataatgt tatttttgat acatacgcgt tataaatatc
    13261 aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat tattcaattt
    13321 atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat aaagattaaa
    13381 aatattttca gtgaatatga aattattgct atttctatta caaagatgtc ctggtctctc
    13441 tttcattctc ttcttgtcaa catcacgaca actttcgtaa aacactcctg ccccgcttca
    13501 ggtatactgt gcaccttaat tccacaaaca tcaggcagac catgacagac ttaatccaac
    13561 gccctcgtcg cctacgcaaa tctcctgcgc tgcgcgctat gtttgaagag acaacactta
    13621 gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac tacaaagccg
    13681 ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc gaaattgaac
    13741 gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac cataccgatg
    13801 aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg cgcatctgca
    13861 agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa tacacttctc
    13921 acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact ctggaaaatt
    13981 taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc ccttccgccg
    14041 cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc tttaaagata
    14101 cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg ttccgtgaag
    14161 ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca atgaaccgtc
    14221 gcgaggcgat tcgtgaatca ctgctggatg aagcccaggg cgcagactgc ctgatggtta
    14281 aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact gaattgccga
    14341 ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg ctggcgggtg
    14401 ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt gcgggtgcgg
    14461 atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg cgttaatttt
    14521 tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta
    14581 accctctccc caagggggcg agggaaccgt ccgcactcgt atcaccccgc ccgataaaac
    14641 ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag gtaatcggca
    14701 ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg ttgccagcgc
    14761 caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa caaggcttcg
    14821 ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac aaacagcgcc
    14881 tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg atttttcgcg
    14941 accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta ttccgggaac
    15001 gatttacgga aatcatgctc cgcacgcagc ccgctcagca gctggcggaa gggaacagag
    15061 agcgcctcat aggccgcaat accgctggtc cagagcgtat caccgccggt cgaaggtaac
    15121 tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc
    15181 cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat ctcgtcaacc
    15241 ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg ggccagcgcg
    15301 cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgata gcgcagcacc
    15361 gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc ggcacccgaa
    15421 atttgtgcgc cgatatacgg ccccagcggg gtaatactca gacgttcact cattgtactt
    15481 ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct aaaagaaagg
    15541 cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga aattcacccg
    15601 ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc gctaccagcg
    15661 tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc agcgcgccgg
    15721 gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg gcggcacgaa
    15781 tgcgaacctg ctgcgcgctt ttcacccccg ccagcaccga catcgccacc ggtgcaaaaa
    15841 tcgctaaata gatcaataag atcttcgagg tttcaccaat accaaaccag atcaccatca
    15901 gcggcaaata agccagcggc ggcaccggac gataaagttc gattaccgga tccagaatgc
    15961 cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc accaccgccg
    16021 cgagtagcgc cagcacaatg cgcgtcaggc tggctgccag atgctgccac agcgtggcgt
    16081 ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt tgcggcggcg
    16141 gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca gtgagtaaaa
    16201 ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc catttcagcc
    16261 gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc gttgctcaaa
    16321 tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact tgatgctgcg
    16381 gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct ccagcacacg
    16441 gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt cttctatatc
    16501 gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca gcagagtttg
    16561 catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta ataacagctg
    16621 gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccac cggaaagctg
    16681 ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct ggtgcgcgat
    16741 ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca cgttgtcctg
    16801 tacattgcgc cacggtagta gcccttcatt ctgaaaaacc accccgcgct ctgctcccgg
    16861 tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca caaaaccggc
    16921 aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca ccagtagctc
    16981 gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat aatcggcata
    17041 gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg aggtaacgta
    17101 ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa actgcgcggt
    17161 gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct gcggcgtcag
    17221 ataggtattc cccttcacca gccccggaac gtcaccttcc ggcacgccgc ttaaacgcgc
    17281 cagtttgctg atgttttccg gctgtttcag ccacgcgtct gggttagcaa tgtacggttg
    17341 ctgagcatcg atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt tctcggcaaa
    17401 atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga cctgttcaga
    17461 atcggtcaac accttgccgt ctttttccag ggcgttaacc gccggtgccc agacataagc
    17521 accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca ggttcacaat
    17581 ctccacttgc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc tgtagtgggt
    17641 ggtggagata aacggtacgg caatgcgttt gccaatcaaa tcttcgggtt tgctgatagt
    17701 tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat
    17761 cggcacctgt tggctggctg caaccgctaa tgggctggaa ccgaggttgc cgatttgcac
    17821 gtcgccagaa gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac
    17881 gctcgccccg ctctctttag caaaggtgtt gtcggcctga gccactttcg ccggttcggc
    17941 tgaggtttga tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag
    18001 tgcggcaaga agtgtgttac gcgatgaaat tgccatgatt gtctgctccc ctgtcttgtt
    18061 atgggagcag tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgcacaga
    18121 tgattttatt cttagcaaaa aaacggcaaa ggaaagatt
//
LOCUS       DAEEBB010000085        17966 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEEBB010000085 DAEEBB010000000
VERSION     DAEEBB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.085"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="KLF07_002666"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="KLF07_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBD9832654.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="KLF07_002667"
     CDS             1598..4654
                     /locus_tag="KLF07_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD9832655.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="KLF07_002668"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="KLF07_002668"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBD9832656.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="KLF07_002669"
     CDS             5161..5349
                     /locus_tag="KLF07_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBD9832657.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="KLF07_002670"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="KLF07_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBD9832658.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="KLF07_002671"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="KLF07_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBD9832659.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="KLF07_002672"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="KLF07_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBD9832660.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="KLF07_002673"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="KLF07_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBD9832661.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="KLF07_002674"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="KLF07_002674"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBD9832662.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="KLF07_002675"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="KLF07_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBD9832663.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="KLF07_002676"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="KLF07_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBD9832664.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="KLF07_002677"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="KLF07_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBD9832665.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="KLF07_002678"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="KLF07_002678"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBD9832666.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="KLF07_002679"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="KLF07_002679"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBD9832667.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            15805..16431
                     /locus_tag="KLF07_002680"
     CDS             15805..16431
                     /locus_tag="KLF07_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBD9832668.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            16452..>17966
                     /locus_tag="KLF07_002681"
     CDS             16452..>17966
                     /locus_tag="KLF07_002681"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBD9832669.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIG"
BASE COUNT         4588 a         4256 c         4726 g         4396 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 aatcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaattgcg gtatgtattt taatctataa cgataatatt
    15481 atttttattt tctgcaatga acgtcacaaa taattgtcgc ttaagttaaa tttatttcaa
    15541 ttgataataa atatcagttc acttataatt tcttgctttg taattttaat tgataacgat
    15601 caatgttaag ggctcagaat aatcacatta ttttttctcc taaccataaa ggattggttt
    15661 atcggcgtta aatcttttct catacactat cgggttaggg aaaatatctg gtgttgggga
    15721 cagagtcagc agcgtattag ttgaatgctt aatatcaggc tgctgaattg tcagcactgt
    15781 gtttgcgaag aggatgcgaa ataaatgaac ccggttgatc gtccactatt agatattggc
    15841 ttaacgcgat tagaattctt gcgtatatca ggaaaaggcc ttgctggtct aacaattgcc
    15901 cctgcgttac tgtcactttt gggttgcaaa caggaagata ttgatagcgg cacagtaggg
    15961 ttgataaata cacccaaagg ggtgctggtg acccagcgag cgcgatgcac gggttgccac
    16021 cgctgtgaaa tctcttgtac caacttcaac gatggctcag tagggacatt cttctcccgt
    16081 atcaaaatcc atcgcaatta tttctttggc gacaacgggg ttggctctgg cggcggcctg
    16141 tatggcgatc tcaactatac cgcggacacc tgccgtcaat gcaaagagcc gcaatgcatg
    16201 aacgtctgcc cgattggtgc gattacctgg cagcagaaag aaggctgtat taccgtcgat
    16261 cataaacgct gtattggctg tagcgcctgt actacggcat gcccgtggat gatggccacc
    16321 gtaaataccg aaagtaaaaa atcctcgaaa tgtgtgttat gcggtgaatg cgcaaacgcc
    16381 tgcccaacag gggcgttaaa aattatcgag tggaaagata ttactgtgtg aatcttgcaa
    16441 aggaaaaaat catggctaac ggttggacag gtaatatatt aagagtcaat ctcacgacag
    16501 gaaatattac cctcgaagat tccagtaagt ttaaaagttt tgtcggtggc atgggcttcg
    16561 gctacaaaat tatgtatgac gaagtaccgc caggcacgaa acctttcgat gaagcgaata
    16621 aattagtctt tgctaccggc ccattaactg gatctggtgc cccctgtagt tctcgcgtaa
    16681 atatcacctc actttctact tttaccaaag gaaatttagt cgtcgatgcc catatgggtg
    16741 gcttttttgc agcgcaaatg aaattcgctg gatacgacgt cattattatc gaagggaagg
    16801 cgaaatcacc ggtatggctg aagattaaag atgacaaagt tagcctggaa aaagccgatt
    16861 tcttatgggg aaaagggacg cgcgcaacga cggaagaaat ttgtcgattg accagtccgg
    16921 aaacctgtgt ggcggctatt ggtcaggctg gggaaaacct tgttcctctc tctggcatgt
    16981 tgaatagccg taaccacagc ggcggtgcgg gaactggcgc aataatgggt tcgaaaaacc
    17041 tgaaagcgat tgcggttgaa gggacgaaag gggtcaacat tgccgatcgt caggagatga
    17101 agcgtctcaa cgattacatg atgactgaac ttattggtgc gaataacaac catgtcgtgc
    17161 caagtacgcc gcaatcgtgg gcagagtatt cagatcccaa gtcacgttgg acagcacgta
    17221 aagggctgtt ttggggcgcg gctgaaggtg gtccgattga aacgggggaa attccgccag
    17281 gcaatcagaa cacggtcggc tttcgtacct ataaatccgt ttttgactta ggtccggcgg
    17341 cagagaaata cacagtaaaa atgagtggct gccactcttg cccgatccgt tgtatgaccc
    17401 aaatgaatat tcctcgggtg aaagagtttg gcgtgcccag cacaggtggt aacacttgtg
    17461 tagccaactt tgtccatacc accatcttcc cgaacggtcc gaaagatttt gaagataaag
    17521 acgatggtcg tgtgattggt aacctggtgg gtctgaatct gttcgatgac tacggcctat
    17581 ggtgtaacta cgggcagttg catcgcgact ttacctattg ttacagcaaa ggtgtgttca
    17641 agcgtgttct gcctgctgaa gagtatgcgg aaattcgctg ggatcaactg gaagcgggtg
    17701 acgttaactt cattaaaatt aaagattttt actaccgtct ggcgcatcgt gtgggtgagc
    17761 tgagtcacct ggctgatggt tcatatgcca tcgcagaacg ctggaatttg ggtgaagagt
    17821 actggggcta cgcgaaaaat aaactctggt cgccgtttgg ctatccggtt caccatgcca
    17881 atgaagcgtc agcgcaggtc ggttccattg ttaactgtat gttcaaccgt gactgcatga
    17941 cgcataccca tatcaacttt attggt
//
LOCUS       DAEEBB010000086        17448 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEEBB010000086 DAEEBB010000000
VERSION     DAEEBB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.086"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..502)
                     /locus_tag="KLF07_002682"
     CDS             complement(<1..502)
                     /locus_tag="KLF07_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD9832670.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="KLF07_002683"
     tRNA            complement(688..772)
                     /locus_tag="KLF07_002683"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="KLF07_002684"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="KLF07_002684"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBD9832671.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="KLF07_002685"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="KLF07_002685"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBD9832672.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="KLF07_002686"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="KLF07_002686"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBD9832673.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="KLF07_002687"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="KLF07_002687"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBD9832674.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="KLF07_002688"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="KLF07_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBD9832675.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="KLF07_002689"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="KLF07_002689"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBD9832676.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="KLF07_002690"
     CDS             7117..8619
                     /locus_tag="KLF07_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBD9832677.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="KLF07_002691"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="KLF07_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD9832678.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="KLF07_002692"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="KLF07_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD9832679.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="KLF07_002693"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="KLF07_002693"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBD9832680.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="KLF07_002694"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="KLF07_002694"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBD9832681.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="KLF07_002695"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="KLF07_002695"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBD9832682.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="KLF07_002696"
     CDS             complement(16186..17382)
                     /locus_tag="KLF07_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBD9832683.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4337 c         4453 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagcatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAEEBB010000087        17293 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEEBB010000087 DAEEBB010000000
VERSION     DAEEBB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.087"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            92..1132
                     /locus_tag="KLF07_002697"
     CDS             92..1132
                     /locus_tag="KLF07_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBD9832684.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="KLF07_002698"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="KLF07_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBD9832685.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="KLF07_002699"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="KLF07_002699"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBD9832686.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="KLF07_002700"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="KLF07_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBD9832687.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="KLF07_002701"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="KLF07_002701"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBD9832688.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="KLF07_002702"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="KLF07_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBD9832689.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="KLF07_002703"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="KLF07_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="KLF07_002704"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="KLF07_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBD9832690.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="KLF07_002705"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="KLF07_002705"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBD9832691.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="KLF07_002706"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="KLF07_002706"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBD9832692.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="KLF07_002707"
     CDS             complement(12899..14080)
                     /locus_tag="KLF07_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBD9832693.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="KLF07_002708"
     CDS             14335..15564
                     /locus_tag="KLF07_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBD9832694.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="KLF07_002709"
     CDS             16024..16656
                     /locus_tag="KLF07_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9832695.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16990..>17293
                     /locus_tag="KLF07_002710"
     CDS             16990..>17293
                     /locus_tag="KLF07_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9832696.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3975 g         4226 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagttttacg
    16861 ttgcaacatt aaagcctcat ttcaatcatc atgataaata taaaattaat atatatttat
    16921 gccgtaaata ccaatatact tagcaaacta tgtgatctcc atttcgattg atttagtgtt
    16981 tattgacgta tgtactggat tattaacgat aatatcgagt tctggcctga gcaccgaaaa
    17041 ttaatatcgg tacataacgc cgatcttaac gtcgttctga caacgccagc cagtcgatgt
    17101 ttatcacttc tacttgaagc ttttcctgat gtggttgcac aacaagattt tttcaccaga
    17161 gtctgggaag aagaaggtat gcgtgtgcct actaacacgt tatatcagaa catatccatt
    17221 atcagacgcg gatttcgcgc tgttggtgat actacccact cgctaattgc aaccgtgccg
    17281 agagaggatt caa
//
LOCUS       DAEEBB010000088        17144 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEEBB010000088 DAEEBB010000000
VERSION     DAEEBB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.088"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KLF07_002711"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KLF07_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD9832697.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KLF07_002712"
     CDS             2219..2767
                     /locus_tag="KLF07_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBD9832698.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KLF07_002713"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KLF07_002713"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBD9832699.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="KLF07_002714"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="KLF07_002714"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBD9832700.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="KLF07_002715"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="KLF07_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBD9832701.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLF07_002716"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLF07_002716"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBD9832702.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="KLF07_002717"
     CDS             complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="KLF07_002717"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBD9832703.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9769..12381
                     /gene="pepN"
                     /locus_tag="KLF07_002718"
     CDS             9769..12381
                     /gene="pepN"
                     /locus_tag="KLF07_002718"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBD9832704.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="KLF07_002719"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="KLF07_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD9832705.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="KLF07_002720"
     CDS             complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="KLF07_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBD9832706.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="KLF07_002721"
                     /pseudo
     CDS             complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="KLF07_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="KLF07_002722"
     CDS             complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="KLF07_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBD9832707.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="KLF07_002723"
     CDS             complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="KLF07_002723"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBD9832708.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4299 a         4421 c         4350 g         4074 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
     8281 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8341 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8401 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8461 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8521 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8581 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8641 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8701 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8761 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8821 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8881 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8941 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     9001 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     9061 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     9121 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     9181 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9241 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9301 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9361 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9421 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9481 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9541 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9601 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9661 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9721 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9781 ccacaagcca aataccgtca cgattatcgt gcgccagatt accagattac tgatattgac
     9841 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9901 catggtgcat cagatgctcc acttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9961 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcattttac gcttaagatc gttaatgaaa taagcccggc ggcgaatacc
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    10261 ggtgaactgg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgacacctt taccacgcgt
    10381 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactcaatg aaatgggatg aagaacgctt cggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccgattg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagtg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catcgacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg aaatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggaggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct gtacgagaag
    12361 atcaccaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ccaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctactg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatctcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac ctgccagcag gcgcagcagg
    13201 gtacttttgc caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcataccgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc cgtttgcaaa aactcccgcg
    13501 cattcatcgc cagataacca atgcctgaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcaccag
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatttcca
    13681 ccagaccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaatagcg
    13741 tgccgagcgc caccagaaag atttttgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggtacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 gttctcccca gcgtgacagc ccgctaatca gtcccagaat caagcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgctgccac aattcgccgc
    13981 tggcggagag cgtccagaac gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg
    14041 acagccagcc aaccgaggag gccagttgcc acaccgccac gatgcccacc ggtaaaaacc
    14101 agggggcaac gcgcaataac cacttcttca ctggcgttgc catcatcgtg ttccttagct
    14161 ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg gattcagcgg
    14221 ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca actcgccaac
    14281 ccgatacgct tcttccagat gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc
    14341 ggcgtattcg ttgattcgcg cggcgaccgt aggaccatcg cccaccagcg ccgttccggc
    14401 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
    14461 cttgccgtta tgtagcgccg ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc
    14521 ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct cggcggcctg
    14581 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga aacgaatttt
    14641 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta ccagctctgg
    14701 cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctcct gggcgacatc
    14761 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
    14821 tgctccccgc acatgaatat gtttaccgtt gaaatcgacg ctttccccca gcaataagcg
    14881 ccccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
    14941 acgccgtcgc ctgccaactc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
    15001 ccatttgaga gacggtcaag cgtggcggcc tggcgagcgg caacggtagg tgaggttacg
    15061 ctgggacgca gggcgacaag aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc
    15121 accagccacg catcttcaca cgatcgcccc gttgggatca gcacaccggt atagcccaga
    15181 cgatccgccg cttgcgcaat ttgttgcaga tagccgtgat caaccgggcg tgaaccttct
    15241 tccgttccaa gataatgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
    15301 ataattgttt tccttccagg tgagtgggct gccagatgcg ctggcgaata tcaacttttt
    15361 tcgacaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
    15421 cagcgctcac cggtttgatg gtggtgggag gacgatgatc cagatacgag gcaatcaccg
    15481 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgctcgcgc tggctgcggg
    15541 ttaacgcatc ggcctcacta aaggttgcca gtacgccctg aataaaagcg ccgttttttt
    15601 ctgcataggg gcgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
    15661 tcagcacccg cacgccgccc tgtaataacg cagcggagta gtagggatcc cagatagccc
    15721 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc agctggcgtc agataagtgg
    15781 gctggatgtc agtaaactta agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg
    15841 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
    15901 ggctgttttc tgccaccaga atcacttcgg ctttgggctt cggtggctcg acgcccacat
    15961 atatcaaatc agccccggca gcctgggcaa agattggcgg aatatccccg gtactgccga
    16021 gatcaatact gccaacgttt aacgcttcca acatttgcgg acccgcgggg aactccaccc
    16081 aggagatttt tgattgcgga tagcgttttt ccagtaactg gtggcttttt gccaatacca
    16141 taccaatact gcctttctga tagcctatac gtaacgcttc aggcgaggat tctgcagcaa
    16201 ccgcaaaagt agagacgcta agcaatcccg ccagcgccag tttaatgatg ttacgcatgg
    16261 acattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caatgcctgc
    16321 cagaaagttt ctagcgcggt atcaagacgg gtttgcagat ttggcgtgaa ctggggtttg
    16381 tgatggtaat caataacttg tgagtcatcg gcaaacacgc cgtgcaggag ctcctgggct
    16441 ttcatcgcac ttaaaactgg cttaagggca taatcgaccg ccagcagatg agccaccgta
    16501 ccgcccgtcg ccagcggtag caccactttg ccttgcaaag cgcgctctgg cagcaggtcg
    16561 agcagggttt tcagcgcgcc ggaataggcg gctttataca caggtgtagc gactatcagt
    16621 ccatcagcct gttgcagctg ttcggtgaag gtcttgagtg ccggactatc gaaacgagcg
    16681 taaagcagat cttccggggc gaagttttgc agattccagt gataaacctc tacatccagg
    16741 ccatttagtt tttcccgcgc atattccagc aaggagctgg agcgagaagg aaagcgagga
    16801 ctacccgcca gggtgatgac acgcatactc tctccttata accaattgtt ctttttttgt
    16861 taacattgat aacaattcgg tcagtctgtc ggagagacaa gaaaatgcca aatataaatt
    16921 ttgtgtatct ttttctgaaa ataaactaaa gaaagggctt tatatatcac gcatatttat
    16981 ttgttggata gtcattagat gttgaatcaa tgaattactc gttcagttaa tatttaaata
    17041 ttcatgaaat ctataaatta aagatttgtc acttattgga tttagtatgc ttgatttaaa
    17101 tggcggtcaa ttattgttat tattaagtct aatgtcattt aaag
//
LOCUS       DAEEBB010000089        16742 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEEBB010000089 DAEEBB010000000
VERSION     DAEEBB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.089"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="KLF07_002724"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="KLF07_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBD9832709.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="KLF07_002725"
     CDS             complement(2362..2802)
                     /locus_tag="KLF07_002725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD9832710.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="KLF07_002726"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="KLF07_002726"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBD9832711.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="KLF07_002727"
     CDS             3520..4218
                     /locus_tag="KLF07_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD9832712.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="KLF07_002728"
     CDS             4447..5655
                     /locus_tag="KLF07_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9832713.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="KLF07_002729"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="KLF07_002729"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBD9832714.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="KLF07_002730"
     tRNA            7762..7838
                     /locus_tag="KLF07_002730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="KLF07_002731"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="KLF07_002731"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="KLF07_002732"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="KLF07_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBD9832715.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="KLF07_002733"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="KLF07_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBD9832716.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="KLF07_002734"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="KLF07_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBD9832717.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="KLF07_002735"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="KLF07_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBD9832718.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="KLF07_002736"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="KLF07_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBD9832719.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="KLF07_002737"
     CDS             complement(14622..15893)
                     /locus_tag="KLF07_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD9832720.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16369..16476
                     /locus_tag="KLF07_002738"
     CDS             16369..16476
                     /locus_tag="KLF07_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD9832721.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         3986 a         4161 c         4520 g         4075 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg
     7861 agcaggcttt tttgcatttg tagctcatga ggatgagaac ctccgggggc agggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    16201 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    16261 cccccgcacg ttgcaggtat gtacctgaca acataacggg ggctaatctt gaacctagac
    16321 cactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
    16381 gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
    16441 agtatgatcg tgaactggct gaacaagcgg aagtagcgtg tcatgtgggc gtcaggctgc
    16501 cgtaatggca atttgcgccc ggaccaggcc gcaggagtga aactctgcgg cctttttcgt
    16561 aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa
    16621 gatgagacgg attccggaac agaaaaacag tcgtagaatc agcgtcaagc cggaaggatt
    16681 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga
    16741 aa
//
LOCUS       DAEEBB010000090        16592 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEEBB010000090 DAEEBB010000000
VERSION     DAEEBB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.090"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..586)
                     /gene="araH"
                     /locus_tag="KLF07_002739"
     CDS             complement(<1..586)
                     /gene="araH"
                     /locus_tag="KLF07_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBD9832722.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
     gene            complement(601..2115)
                     /gene="araG"
                     /locus_tag="KLF07_002740"
     CDS             complement(601..2115)
                     /gene="araG"
                     /locus_tag="KLF07_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBD9832723.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2185..3174)
                     /gene="araF"
                     /locus_tag="KLF07_002741"
     CDS             complement(2185..3174)
                     /gene="araF"
                     /locus_tag="KLF07_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBD9832724.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3971..4474
                     /locus_tag="KLF07_002742"
     CDS             3971..4474
                     /locus_tag="KLF07_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBD9832725.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4553..4804)
                     /locus_tag="KLF07_002743"
     CDS             complement(4553..4804)
                     /locus_tag="KLF07_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBD9832726.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="KLF07_002744"
     CDS             complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="KLF07_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBD9832727.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5268..5591
                     /locus_tag="KLF07_002745"
     CDS             5268..5591
                     /locus_tag="KLF07_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBD9832728.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5762..6259
                     /gene="ftnA"
                     /locus_tag="KLF07_002746"
     CDS             5762..6259
                     /gene="ftnA"
                     /locus_tag="KLF07_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBD9832729.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6297..6536)
                     /locus_tag="KLF07_002747"
     CDS             complement(6297..6536)
                     /locus_tag="KLF07_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBD9832730.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6693..7937
                     /gene="tyrP"
                     /locus_tag="KLF07_002748"
     CDS             6693..7937
                     /gene="tyrP"
                     /locus_tag="KLF07_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBD9832731.1"
                     /translation="MTHKDRQDRRKRENRTLGSVFIVAGTTIGAGMLAMPLAAAGVGF
                     SVTLILLIGLWALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLM
                     YALTAAYISGVGELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFL
                     FSAKIIFLVVMLVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYM
                     DGNVRKLRWVFITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREM
                     VASPHVELAVHLFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPL
                     AFALFYPRGFVMALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVF
                     LCGIAVIGVQFLIAAGLLPEVG"
     gene            complement(7999..8664)
                     /gene="yecA"
                     /locus_tag="KLF07_002749"
     CDS             complement(7999..8664)
                     /gene="yecA"
                     /locus_tag="KLF07_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBD9832732.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8860..8946)
                     /locus_tag="KLF07_002750"
     tRNA            complement(8860..8946)
                     /locus_tag="KLF07_002750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8910..8912),aa:Leu,seq:taa)
     gene            complement(8959..9032)
                     /locus_tag="KLF07_002751"
     tRNA            complement(8959..9032)
                     /locus_tag="KLF07_002751"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8998..9000),aa:Cys,seq:gca)
     gene            complement(9087..9162)
                     /locus_tag="KLF07_002752"
     tRNA            complement(9087..9162)
                     /locus_tag="KLF07_002752"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9127..9129),aa:Gly,seq:gcc)
     gene            complement(9314..9862)
                     /gene="pgsA"
                     /locus_tag="KLF07_002753"
     CDS             complement(9314..9862)
                     /gene="pgsA"
                     /locus_tag="KLF07_002753"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBD9832733.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9919..11751)
                     /gene="uvrC"
                     /locus_tag="KLF07_002754"
     CDS             complement(9919..11751)
                     /gene="uvrC"
                     /locus_tag="KLF07_002754"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBD9832734.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11748..12404)
                     /gene="uvrY"
                     /locus_tag="KLF07_002755"
     CDS             complement(11748..12404)
                     /gene="uvrY"
                     /locus_tag="KLF07_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBD9832735.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12700..12876
                     /locus_tag="KLF07_002756"
     CDS             12700..12876
                     /locus_tag="KLF07_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832736.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12863..13087
                     /locus_tag="KLF07_002757"
     CDS             12863..13087
                     /locus_tag="KLF07_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBD9832737.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13154..13876)
                     /gene="sdiA"
                     /locus_tag="KLF07_002758"
     CDS             complement(13154..13876)
                     /gene="sdiA"
                     /locus_tag="KLF07_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBD9832738.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(14106..14858)
                     /gene="yecC"
                     /locus_tag="KLF07_002759"
     CDS             complement(14106..14858)
                     /gene="yecC"
                     /locus_tag="KLF07_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBD9832739.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(14855..15523)
                     /gene="tcyL"
                     /locus_tag="KLF07_002760"
     CDS             complement(14855..15523)
                     /gene="tcyL"
                     /locus_tag="KLF07_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBD9832740.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15538..16524)
                     /gene="dcyD"
                     /locus_tag="KLF07_002761"
     CDS             complement(15538..16524)
                     /gene="dcyD"
                     /locus_tag="KLF07_002761"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBD9832741.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         4188 a         4277 c         3843 g         4284 t
ORIGIN      
        1 aggtggtttt attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg
       61 gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac
      121 cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca
      181 gtgttgtgat cagagcattt attttcagtt tggcgataac aaagccattg accaggccac
      241 agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga
      301 taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt
      361 caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca
      421 ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga
      481 taaagagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga
      541 atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac
      601 tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc
      661 ctgctcgtgt aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc
      721 aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc
      781 cagcgcataa attacgttat atatttcgtg cttagcacca acatcaatgc tgcgcgtagg
      841 ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg
      901 ctgatttccg cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag
      961 cgaacgaatg tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc
     1021 gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat
     1081 aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct
     1141 cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt
     1201 cccgccaaac aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc
     1261 aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac
     1321 agcatcaaga cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc
     1381 aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt
     1441 aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat
     1501 ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat
     1561 aacgcggaaa agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc
     1621 gataatttta gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat
     1681 ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa
     1741 acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc
     1801 gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat
     1861 cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat
     1921 cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt
     1981 tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat
     2041 atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg
     2101 ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat
     2161 gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc
     2221 tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc
     2281 aacgtctttt gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg
     2341 gcttggcagc agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgctcaacgc
     2401 atccacaccg ttaatgccaa tgccgatgat atcggcagct ttaaagccct gaccttccgt
     2461 cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac
     2521 ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga
     2581 tttggtaggt acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat
     2641 agaacccgtt gtacggcgac gtgccgtatc cagttcgttg gcggtaatcg ccatcaccgc
     2701 gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg
     2761 ttcgccaatt ttagtcgccg ccatcatcac cagcggaacg gtatccattg gcttaccttt
     2821 ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac gcgctttcgc
     2881 gacgatggca gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact
     2941 ggcagccagg ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat
     3001 aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca
     3061 cggctcttcc ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg
     3121 tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct
     3181 ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gactttcgtt aaattaatcg
     3241 gaaaacaaag cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg
     3301 agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca
     3361 taaatttagc gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca
     3421 cagcagatta atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg
     3481 atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt
     3541 gtaataaccg caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc
     3601 actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa
     3661 cgttttataa aatcatcact tcatcatgaa ttcaaattca ttgattaata tcaacaagat
     3721 acaaaaagca ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa
     3781 aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt
     3841 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
     3901 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
     3961 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
     4021 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
     4081 cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
     4141 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
     4201 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
     4261 ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
     4321 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat
     4381 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
     4441 tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc
     4501 cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg
     4561 attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca
     4621 tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg
     4681 acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga
     4741 taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg
     4801 acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag
     4861 cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt
     4921 atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac
     4981 ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat
     5041 tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc
     5101 acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg
     5161 acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
     5221 gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga
     5281 ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga
     5341 gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc
     5401 atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
     5461 gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
     5521 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
     5581 tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa
     5641 tgcgttttat tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat
     5701 aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat
     5761 catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc
     5821 ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc
     5881 cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta
     5941 cctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata
     6001 ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa
     6061 aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct
     6121 gcagtggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa
     6181 attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac
     6241 cctcgacaca caaaactaat gctctccggc ggcaggttta ctgccgccgt ttcaaatcag
     6301 tgacggcaaa tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct
     6361 tttgctgcca gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga
     6421 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
     6481 tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
     6541 cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagcgcag
     6601 gactgaatgc tttttattgt acatttatat ttacactata tgtaacgtcg gtttgacgaa
     6661 gcagccgtta tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa
     6721 gaaagcgtga aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg
     6781 caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct
     6841 tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc
     6901 agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg
     6961 gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca
     7021 tcagcggtgt cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt
     7081 cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgttggaa
     7141 catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg
     7201 tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc
     7261 aacaggggct ggctctgtca gcaatcccgg tgatttttac ctcatttggt tttcacggta
     7321 gcgtgccgag tattgtcagc tatatggatg gcaacgttcg taagctacgc tgggtgttta
     7381 taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggca acgcttggca
     7441 gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta aacgggctgt
     7501 tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg
     7561 ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg
     7621 ctgatttatt tcagcgttca aataccgttg gtggacggtt gcaaactggt gcaattacct
     7681 ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt
     7741 acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa
     7801 gcagaaagca caatcctcag gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg
     7861 tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt
     7921 taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag aattaacgct
     7981 gacacccaat ttggcctctt aatgcaggca gcactgctta aatttcttac cactaccgca
     8041 cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt
     8101 ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc
     8161 tacgctctct tcaaacgctt ctggcgacat cttttctacc cgctcgaagt tttcctcagt
     8221 accgtgcagc gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc
     8281 agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct
     8341 gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg
     8401 ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc
     8461 tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc actgttccgg
     8521 ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc
     8581 aaggatggcg tggttagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact
     8641 ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct
     8701 atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa
     8761 ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg
     8821 acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg
     8881 aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat
     8941 ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc
     9001 aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat
     9061 ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc
     9121 gaccttggca aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt
     9181 taatcacttg attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct
     9241 acttacctgg cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat
     9301 tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca
     9361 ttgaccacag agtcagtacc gcagccacaa agaaaagtgc aataccggcg tactcaaccc
     9421 aaatgttcgg acgccacagc agccatgcca acgccaccat ctgggcagtg gttttcactt
     9481 tcccaatcca ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta
     9541 gcgcagaaat aataatttca cgggcgatca tcgttgctgc cggtaaggtc acccaccagc
     9601 tgtggtaatg ctcggttacc agcaccatgg cgatagccac gagaacttta tctgctacag
     9661 ggtcaaggaa agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga
     9721 accagtcagt caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga
     9781 caggcagata aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca
     9841 acgtagggat attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc
     9901 tatgttgcta cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg
     9961 cgaaataccc ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc
    10021 gcccatatat ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag
    10081 ggaactggta tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg
    10141 tgattcatcg cgaatatgct ggataacatg cagcgcggga gaatctggcg gcaaactgaa
    10201 tccctcacct tccggctcaa agaacagcgt ttccagccca gccttacgat ctgctccttt
    10261 ggcaacgcca agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac
    10321 atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt
    10381 actgtcgtca atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc
    10441 atcgcccggc gtgatgcctg taatgttata gcgccgatac tccgcacgca gcgggccgtt
    10501 agcatcaaac accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa
    10561 gcactccatc cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg
    10621 gtgaacggta gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc
    10681 gagtttcaga taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc
    10741 cgccagttct gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag
    10801 gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa tagaactggc ctacgaaggt
    10861 ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc
    10921 gagcactttg ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc
    10981 cacaccaata acgtcgaggt cgtcgccggt attggaaacg aattgttttt cggtgacgcg
    11041 tcgcaccgcc tgaatttggt cgcgaatacg tgcagcttct tcaaactcca gattctggct
    11101 ggcagtttcc atacgactaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa
    11161 caggcgcaca tactcaacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca
    11221 cggtcccaga cagcgcccta tctgatattg cagacacgga cgcgagcgat tgcgataaac
    11281 actattttcg cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac
    11341 ggcatagcca ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg caccacgatg
    11401 catcgccaga cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc
    11461 gcgtagcaaa acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag
    11521 cgcttcggtt tctgtatgcg taaccgttac atcaatttgc tggatctggg cgaccagcgc
    11581 ttcggttttg cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt ttttcaggtc
    11641 tttcgctttg ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg
    11701 ctggcaggtt acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat
    11761 aatgtctccg cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca
    11821 tgaatgttta gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga
    11881 ttgagctgtt ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc
    11941 aattcacgtt cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt
    12001 aacgccattt gttgagcgat atcagaagca atgtaacgct gccctgaata gacagaacga
    12061 atcgcactca cgacttcctg cggagccgcg cctttgctga ggtagcccgc agcaccggcc
    12121 tgcatgactt tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca
    12181 tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc
    12241 atgtccatta gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg
    12301 cacgatgcct caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct
    12361 gcgcgcacca gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa
    12421 atagggataa cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt
    12481 gaaacgttaa aaatggtggg tattcgattt ttctctcgtt tttgtaaatt cgattgtcca
    12541 cagttatggt cacgcccgtc accaacggct tccgggatcg tttcacagta agcccggcga
    12601 tgaaatgtga caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt
    12661 aacattaatt aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt
    12721 cattatatta acgcatttaa tagcattgtt gacgtcacgc tcaaaaaaca accactgcta
    12781 tttttagcaa agcttatggt atactccgcc gccttaacat ttttcaccgc aaattttcat
    12841 tgcaacatga cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca
    12901 agaactggca aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tacaggcgat
    12961 gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga
    13021 agacgaaacc caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg
    13081 tcagtaatat aaaaccggct gagagcgtgc ctttcagccg atttttgcat ctggcacgca
    13141 gaaagaaaag cgatcaaatt aagccagtag cggccgcgta acaggccacc tgggtcttat
    13201 ttggtgcatt gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga
    13261 ttgacaaaat catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcagaattt
    13321 ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca
    13381 tcagagccat cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa
    13441 gaatgggtat ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccagc gcccgatttg
    13501 gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgtgcgcg gcttcccata
    13561 atggctgtgc ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt
    13621 cagggttcag cacaggatca attgcgagaa aattttttgc ctgataataa ctaacccacg
    13681 actcagggta attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa
    13741 cacataacga atagtaatcg tactccagct gctgagcctg aagctctatt tcatggtaga
    13801 cctcttctgc ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa
    13861 aatccgtatc ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat
    13921 aattcaaact atcacataaa taagatttat atataattta tattattcag gcaatgaatt
    13981 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca
    14041 gttttatcac gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt
    14101 tcttattatt gcagtagaaa cttctcgagg aactggcggg tgcgaggctg ctggggatca
    14161 gcaaataacg cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc
    14221 cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt
    14281 tcctgcgcca gctggcggat tgtgttcagg acttcgccca ccagctctgg atccagcgct
    14341 gacgttggct cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg cgcaatcgct
    14401 acacgctgct gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc
    14461 ccaactttcg ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
    14521 acgatcaccg gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag
    14581 ttctggaaga caaacccgac gtgctgacgc aactggcgga tcagcgattt ttgctgactt
    14641 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt
    14701 tccagcagat ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt
    14761 gccaccactt cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga
    14821 aattttttca ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat
    14881 taagttgatt ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga
    14941 tgagcgaagc cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg
    15001 cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatcc tttaccaggc
    15061 tgataaagct gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc
    15121 gacgcatagt ctgccatggc gtcataccaa tactggcagc tgcttcccac tgacctttat
    15181 caatagaaga aatggcggcg cgcagtgttt cggcggcata ggcggcagtg ttcagcgaca
    15241 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat
    15301 agatcataaa cagttgtgcg atgagcggtg taccacgaaa aatagagata taaaaacgcg
    15361 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca
    15421 gtaagccaaa aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca
    15481 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
    15541 aacgtgggga tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg
    15601 cccttcatct ttgaagcgtt tctgactgat acagccaatc agccccgcca tcgcttttcc
    15661 ggtatacaca ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat
    15721 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtataatttc
    15781 cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg
    15841 caactgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag
    15901 gtgttccagc ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac
    15961 cgacgaaata ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata
    16021 acctagcgcg ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
    16081 tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcttcgca
    16141 catttcaatc tgggtattga acagatccag caacaagcga ttgccgttgg ttaaatagtt
    16201 ttctgcggtt gtgccaatag gattttccag cagcgccaca cagtgcagac cgagtttcgc
    16261 cgcaactgcg gcagtctgcc gcacatggtt agactggatc accccggcag taatcagcgt
    16321 atcggcacct tcgcgcagag catctgccgc gagaaattct agcttacgta atttattgcc
    16381 gcccattgcc atgggggtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc
    16441 agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa
    16501 acgggttaaa ttatgcagtg gcataacagc ctccgatgta tgttgttgtg attttcttat
    16561 tatgcacgct gaaaacgcgt aaataaaaaa gg
//
LOCUS       DAEEBB010000091        16555 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEEBB010000091 DAEEBB010000000
VERSION     DAEEBB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.091"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="KLF07_002762"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="KLF07_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBD9832742.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="KLF07_002763"
     CDS             433..621
                     /locus_tag="KLF07_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBD9832743.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="KLF07_002764"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="KLF07_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBD9832744.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="KLF07_002765"
     CDS             816..989
                     /locus_tag="KLF07_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBD9832745.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="KLF07_002766"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="KLF07_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="KLF07_002767"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="KLF07_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBD9832746.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="KLF07_002768"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="KLF07_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBD9832747.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="KLF07_002769"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="KLF07_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBD9832748.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="KLF07_002770"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="KLF07_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBD9832749.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="KLF07_002771"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="KLF07_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="KLF07_002772"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="KLF07_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBD9832750.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="KLF07_002773"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="KLF07_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBD9832751.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="KLF07_002774"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="KLF07_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="KLF07_002775"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="KLF07_002775"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBD9832752.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="KLF07_002776"
     CDS             complement(15250..15477)
                     /locus_tag="KLF07_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBD9832753.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="KLF07_002777"
     CDS             complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="KLF07_002777"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD9832754.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16155..>16555
                     /locus_tag="KLF07_002778"
     CDS             16155..>16555
                     /locus_tag="KLF07_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9832755.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         4159 a         4071 c         4226 g         4099 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttatcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcact
    16081 agtattcttg ccttaacact catatcattg ttctcctgtt caatgaacca cttagataag
    16141 catagtccga agatatgaag ctgctagtga catacatcag gtggagtact aaagagcaag
    16201 actcaggaga ctccttaaga cggcaaacaa acctcattga tgctttctac tctaagcaca
    16261 aaaacgatta ttaccttctt cctgctcaca gatatgttga taagggtaaa tctggttttc
    16321 accagcaaca taagaatcaa ggttctgact tcagacgtat gtttgaaaat gttatgtctg
    16381 gggctattcc agaaggttcc ttgattgttg tagagaactt tgaccgcttc agtagggctg
    16441 atatcgacac agctatagat gatgtcaggc agatactgag gaagggggtc tctatactga
    16501 ctctgggtga tggtgaactc tacgacaagt cagccttaac agaccctgtg aagtt
//
LOCUS       DAEEBB010000092        16550 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEEBB010000092 DAEEBB010000000
VERSION     DAEEBB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.092"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(119..271)
                     /locus_tag="KLF07_002779"
     CDS             complement(119..271)
                     /locus_tag="KLF07_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD9832756.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(536..1270)
                     /gene="cysH"
                     /locus_tag="KLF07_002780"
     CDS             complement(536..1270)
                     /gene="cysH"
                     /locus_tag="KLF07_002780"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBD9832757.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1345..3057)
                     /gene="cysI"
                     /locus_tag="KLF07_002781"
     CDS             complement(1345..3057)
                     /gene="cysI"
                     /locus_tag="KLF07_002781"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBD9832758.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3057..4856)
                     /gene="cysJ"
                     /locus_tag="KLF07_002782"
     CDS             complement(3057..4856)
                     /gene="cysJ"
                     /locus_tag="KLF07_002782"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBD9832759.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5175..5537
                     /gene="queD"
                     /locus_tag="KLF07_002783"
     CDS             5175..5537
                     /gene="queD"
                     /locus_tag="KLF07_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBD9832760.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5615..6886
                     /locus_tag="KLF07_002784"
     CDS             5615..6886
                     /locus_tag="KLF07_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD9832761.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6877..7137
                     /locus_tag="KLF07_002785"
     CDS             6877..7137
                     /locus_tag="KLF07_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD9832762.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7154..7729
                     /locus_tag="KLF07_002786"
     CDS             7154..7729
                     /locus_tag="KLF07_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBD9832763.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7877..8737)
                     /locus_tag="KLF07_002787"
     CDS             complement(7877..8737)
                     /locus_tag="KLF07_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBD9832764.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8734..9513)
                     /locus_tag="KLF07_002788"
     CDS             complement(8734..9513)
                     /locus_tag="KLF07_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBD9832765.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9491..10900)
                     /locus_tag="KLF07_002789"
     CDS             complement(9491..10900)
                     /locus_tag="KLF07_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9832766.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10922..12376)
                     /locus_tag="KLF07_002790"
     CDS             complement(10922..12376)
                     /locus_tag="KLF07_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD9832767.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12446..13262)
                     /locus_tag="KLF07_002791"
                     /pseudo
     CDS             complement(12446..13262)
                     /locus_tag="KLF07_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13580..14857
                     /locus_tag="KLF07_002792"
     CDS             13580..14857
                     /locus_tag="KLF07_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9832768.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14884..16369
                     /locus_tag="KLF07_002793"
                     /pseudo
     CDS             14884..16369
                     /locus_tag="KLF07_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3937 a         4356 c         4165 g         4092 t
ORIGIN      
        1 aatatatatg aataataaac ataggcataa tattcacata aataatataa attaaaaaag
       61 ggtgcaggag accaaaccat cacaattcag tacgcggggt tttacccccg cgtggtggct
      121 acttcttcga ttcgtaagcg agaactgcct taaactccat accacgttct ctgatactca
      181 actcacacaa cgagtcgcct accatcagcg tgaatcccag tactgtaaaa cacagcacta
      241 tgattgccac aagggcatat tttgtcagca tgtcttgcct ccctgcaaag aagagactaa
      301 cattcgaatt gtttgggttc gaacgctggc ctcaggttga tagaaatatc gcctggggct
      361 tttgtccatc tggaacctcg cgaatgctta acgccagaca gcctcaagca cccgacgcca
      421 ttctatacct gataattctt ccagcggcgt tcacgtttaa aatcaggaat actgctcgca
      481 aaataaaaac gcccccatca tttctgacag aggcgtttaa tttatccggc aatatttacc
      541 cttcgtgcaa tccacattcc ctttttaagc caaagaaacg tgtttcttct tccgacatgc
      601 caggttccca tttacgggtt gtatgggtat caccgaccga taaatatcct tcatcccata
      661 atgggtgata tttcaggcca tgtttttgca ggtactgata aatagttcgg ttatcccagt
      721 cgataatcgg cagcacttta aatacgccac gctgaattgc cagcaccggt aaattggcgc
      781 gactgccgga ttgttcacgg cgcaggccag caaaccaggt ttgcgcgtta agttctttca
      841 gagcccggtt catcggttcg actttgttga tgtcattgta cttttcaatg ccttcaacgc
      901 cctgctccca cagttttccg tagcgtgctt cctgccaggc tgcgctttcg gtagcacggt
      961 acactttcag gttgagcttg agtttgtccg ttaactcgtc aataaagcgg taggtttccg
     1021 ggaacaagta acccgtatcg gtgaggatca ccggaatatc cgggcgaatt tgattcacca
     1081 gatgcaggct caccgccgcc tgaatgccaa agctggaaga aagtacatat tcaccgggca
     1141 gattatccag cgcccaggct acgcggcctt cagcgtccag tttttccagt tcggcgttag
     1201 tttccgccag cgccagaata cgatctacct tcggcagttc gttcagggcg tttagatcga
     1261 gtttggacat agattcctca ctgtttgcct tgcctgatgc gacgctcacg cgttcttatc
     1321 aggcctacaa gaccgggctg atggttaatc ccacaaatcg cgcgccggat cgagcaccgg
     1381 gcgaatgatg cccgcacgca ccgtaaagtc gccgaagcct tcacccgctt cgcgctcttt
     1441 cgcccagcgc cctatcagtt catcaagcga cgccaggatt tccggctcgg tgatgttttc
     1501 tttatacatc cgtgggatac gtgtcccaat gcggttgccg ccaagatgca ggttgtagcg
     1561 acccggcgct ttacccacca ggcccacttc cgccagcatc gcgcgaccac aaccgttcgg
     1621 gcagcctgtt acacgcatca cgatatgctc atcgctgaca ccatgtttcg ccattaaatt
     1681 atcgatgttg tcgataaaag acggcaggaa acgctctgct tccgccatcg ccagcgggca
     1741 agtcgggaat gacacgcagg ccatcgagtt ttcacgctgc ggcgtgacgg cattcattaa
     1801 cccgctctct ttggcgatct tctcgatctt cgctttctcg ctttccggta caccggtgat
     1861 gatcagattc tggttcgccg taatgcggaa atcgcctttg tggatcttcg cgatctccag
     1921 caggccggtt ttcagcggac gccccggata atcaaggatg cgaccatttt cgataaacag
     1981 cgtcaggtgc cagttatcat caatgccctt aacccagcca atacgatcgc ctcgtccggt
     2041 gaactcatat ggacggatcg gttcaaattt gatccccgcg cgacgctcca cttccgcttt
     2101 aaacgtctca acccccacgc gctccagcgt gtatttggtt ttggcatttt tacgatcggt
     2161 tcggttaccc cagtcacgct gagttgtcac gacggcttcg gccaccgcca gcgtatgctc
     2221 cagcggcaga tagccaaact cgctcgccgt gcgggcgtag gttttcttgt tgccgtgttc
     2281 gatagaaagc ccaccgccca ccagcaggtt gaagcctacc agcttgccgt tttcggcgat
     2341 tgccacgaag ttcatgtcgt tggcatgcag atcgatatcg ttctgcggcg ggatcactac
     2401 cgtggttttg aacttacgcg gcaggtaggt ctgaccaagg atcggttctt catcagtagt
     2461 ggcgactttt tcctggtcga gccagatctc cgcatacgcg cgggtacgag gcaacagatg
     2521 ctcagaaatc ttcttcgccc actcgtacgc ttccgcgtgc agctgcgact cgtaagggtt
     2581 cgaggtgcag agtacgttac ggttcatgtc gttagctgtc gccagcgcat caagaccgac
     2641 cgagtgcagc atctggtgca ccggtttgac gttctttttc agaatgccgt ggaactgaaa
     2701 cgtctggcgg ttggtcaggc gaatgctgcc atagatggtg ttttcaccgg caaatttgtc
     2761 gatcgcctgc cactgtttag tggtaatcac cccacccggc agacgacagc gaagcagcat
     2821 cgcgtggcgc ggctccagct tctgttcagc acgttcggca cggatgtcgc ggtcatcctg
     2881 ctgatacatg ccgtggaagc ggatcagcag gaagttgtcg cccttaaagc cgccggtcag
     2941 accgtcgttt aaatcttccg caatggtgcc gcgcaggtag ttgctttcaa gcttcatgcg
     3001 ctcggcgtct gtcagttttc cttcgaccac taaaggccct ggatgttttt cgctcattag
     3061 tagacatctc gctgataacg gcgctctacg cgcagctcac ttaaaaattc atccgccgct
     3121 tcggtgtcca tgccaccaaa ttcggcaatc acttccagaa gtgcctgctc aacgtctttc
     3181 gccatgcgat tagcgtcgcc gcagacataa atgtgggcac cgtcattgat ccagcgccac
     3241 agctccgcgc cctgttcgcg cagtttgtct tgtacataaa ctttttcttt ttggtcgcgc
     3301 gaccaggcaa gatcgatacg cgtcagcacg ccctctttga cgtagcgctg ccactccacc
     3361 tggtagagga aatcttccgt aaagtgcggg ttgccaaaga acagccagtt tttacccggc
     3421 gcttcgtcgg cggcgcgttg ctgcataaag gcacggaacg gcgcgatgcc ggtaccgggg
     3481 ccaatcataa tcactggcgt ttgcgggtta gcgggcaggc ggaaattgtc gttatgttcg
     3541 ataaatacgc ggacttcgcc ctcttcttcc acgcgatccg cgaggaagct ggaggcacca
     3601 ccggcacggg cgcggccttc cacgtcgtaa cgcaccacac caacggtgac gtgtacttcg
     3661 ttctcgactt ccgcctgcga ggaggcgatg gaatacaggc gcggggtcag cgggcgcagc
     3721 aggttaatca gcgcttcggc atccagttgc gccggagaga aacgcaccat gtcgacaatc
     3781 ggcgtggtcg cggcgtaatg ctgtaacttc gctttatcgc ccaccagcgg cagcagcgtt
     3841 tcgctgcggg taagcgtggc atagttctca acaatgttgg cggtgttgac ggtcagttcg
     3901 aagtgccact gtagcgcttc gttcagaggc aacgttttgc cctcgacggt gacaggttca
     3961 tcgcctttca gccacagcag ttcgacaagt tctttcacca gtgccgggtc gttctgatac
     4021 cagacaccca gcgcgtcacc cggctggtaa cgcaggcctg aatcgcctaa gtcaatttcg
     4081 atatgacgaa cgtctttttc agagttacgc ccggtaattt tctggttaac cgaaaggctc
     4141 gccaccagcg gcgcgtcttt gctgtacggg ctggtgtgga tttcatttac cgcgccagta
     4201 gcgacggatt gcgaaggtgc cgcgacaggc gcacgcgatt taagcgcatc aaccacgcgg
     4261 gcgcgccact cgctggcagc agcctggtat tcaacatcgg catcgacacg gtcgagcagg
     4321 cgttcaccac ccagttccgc cagcttgctg tcgaaatctt tcccggactg gcagaaaaat
     4381 tcataagagc tatcgccgag gctaaacacg gcaaacgcgg tgttttccag ctttggcgct
     4441 tttttggaga acaggaactt atgcagcgcg acggcttctt ccggcggttc cccttcccct
     4501 tgcgttgacg tcactacgat gagcagtttt tcgctggcga tttgtttgaa tttatagtcg
     4561 cccgcgttca ccagcttaac gttcagtttt gctgctaata aatcatcgcg taatgcttca
     4621 gcaacccggc gcgcattgcc ggtttgcgag gcggagataa tagttatacc cggcatttct
     4681 gcggctggcg ctggcgtcgc tgcaagcgca gcaggctgct gattgagtac gccccagaaa
     4741 tagccagaaa cccaggcaag ctgggtaggc gttaaatcgg tcgtggccgc ctgaaggcgt
     4801 gccagttgct ccgggttcaa cggaagcaac gcggaaggtg ggacctgtgt cgtcatgcgt
     4861 cgttatgttc cagtaagcaa agctgtttct gcgccctgtc agcgcccata aaacagaaga
     4921 gaagataagg ttaacggggt gaacggtgtg gattaaagac gggatagcga taactaataa
     4981 ccaaatcgac taacctgttt tagcaatagt ctttaacaac aaaatagatt aaccaaccca
     5041 atgaaaaaca aatgaattta gccaatcatt aagataaatc agcgattttg cgcaacaaag
     5101 tcgttttaga taatgcgaaa aaacagcctt tccggtactc tacggcggtt ttatcgtcct
     5161 gtagagaaat tatgatgtcc accacgttat ttaaagattt caccttcgaa gccgctcacc
     5221 gcttaccaca cgtcccggaa gggcataaat gtggtcgcct gcacgggcat tcctttatgg
     5281 tgcgactgga aattaccggg gaagtcgatc cgcatacggg ctggattatc gatttcgctg
     5341 aactgaaagc ggcgtttaag ccaacttatg agcgcctcga tcaccattat ctcaatgata
     5401 ttccaggtct ggaaaaccca accagcgaag ttttagcaaa atggatttgg gatcaggtta
     5461 aacccgttgt gccgctgtta agtgcggtga tggtaaaaga aacctgcacc gcagggtgta
     5521 tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt tcccttaagt aacgctatgt
     5581 tagggtgttg tgttctggat atctggggca tgacatggaa gacgactgcg acattattat
     5641 tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc tgcgcgcgag cgggtttatc
     5701 cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa aatctttccg gcgggcggtt
     5761 atatacccat gcactcgcgg aactcctccc gcaatttcat ctgaccgcgc ctcttgaacg
     5821 acgcatcact cacgaaagcc tttccctgtt aacgcccgat ggcgtaacga cgttttccag
     5881 cttacagccc ggcggtgaat cctggagtgt attacgtgca cggttcgatc cgtggctggt
     5941 tgccgaagcc gaaaaagaag gtgtcgaatg catccccgga gcgacggtgg atgcactgta
     6001 tgaagaaaac ggcagagtct gtggcgttat ttgtggtgac gatattctcc gcgcccgtta
     6061 tgtggtgctg gcagaaggtg ccaacagcgt cctggctgaa cgtcacgggt tagtgactcg
     6121 tcctgctggc gaagcgatgg cgttggggat caaagaagtg ctgtcgctgg aaacatccgc
     6181 tattgaagaa cgttttcatc tggagaataa cgaaggcgca gcgttgctgt tcagcggcgg
     6241 aatctgtgat gacttacccg gcggcgcatt tctttatact aatcaacaaa cgctctcgtt
     6301 agggattgtt tgcccgctct cttcccttac gcaaagtcgt gttccggcaa gcgagctgct
     6361 gactcgcttt aaagcgcatc cggcagtgcg cccgcttatc aaaaacacgg aatcactgga
     6421 gtatggtgcg catctggagc cagaaggtgg cttgcacagt atgccggtgc aatacgccgg
     6481 taacggctgg ctgctggtgg gcgatgcgtt gcgcagttgc gtcaataccg gaatttccgt
     6541 gcgcggcatg gatatggcgc tgactggcgc acaggcggcg gcacaaacgc taataagcgc
     6601 ctgccagcac cgcgagccgc aaaatctgtt tgcgctttat catcacaacg tagagcgcag
     6661 cctgctatgg gatgttctac aacgttatca gcatgttccg gcgcttttgc aacgccctgg
     6721 ctggtatcgg gcgtggcctg cgttaatgca ggatatttcc cgcgatttat gggatcaggg
     6781 tgataaacct gttccaccgc tgcgccagtt attctggcgt catttacgtc gtcatggcct
     6841 gtggcatctg gcgggcgatg ttatcaggag tctgcgatgt ctgtagccca taatctctgg
     6901 cgcgttgctg atgcgccgca cattgttccg gctgactccg ttgagcgtca gatggtacaa
     6961 cggttgatta acgcctgtcc ggcaggtctt ttttcgctca caccggaagg tgacttacgc
     7021 gttgattatc acggctgcct ggagtgtggc acctgccgtt tgctgtgcga cgaatccaca
     7081 ctacaacagt ggcgctatcc gccttccgga ttcggcatca cctaccgctt tggataaata
     7141 ataaggataa tttatgcccc tcttacacct actccgccag aatccggtga ttgctgccgt
     7201 taaagataat gccagcctgc aactggctat cgattctgaa tgccagttta tttccgtgtt
     7261 gtacggcaat atctgcacca tcagtaatat cgttaaaaag attaagaacg ccgggaaata
     7321 tgcttttatt catgttgatt tgctggaagg cgcgtcaaat aaagaagtcg ttattcagtt
     7381 tttgaaactg gtgaccgagg cggacggcat tatcagtacc aaagcctcaa tgttgaaagc
     7441 ggcaagagcg gaaggttttt tctgtattca tcgcctgttt attgttgatt caatttcgtt
     7501 tcacaacatt gataagcaag ttgcgcaatc gaatccggat tgtattgaga tcctgccagg
     7561 cggtatgccc aaagtgctgg gctgggtgac agagaaaatc cgccaaccgc tgattgccgg
     7621 tgggctggtg tgcgatgaag aagatgcgcg taatgcgatt aacgcgggtg tcgtggcgct
     7681 ttccaccacg aataccgggg tctggacgtt agcgaaaaaa ttactttgac gggataatcg
     7741 taaccaattg aatttggttt gatttgtagg ccgcacgcca catccgacat tcagcgcctg
     7801 atgcgacgct tgacgcgtct tatcaggcct acaagtcccc aggccagaac cgtaggcagg
     7861 atcatccggc aacacgttat tggcaatctg cgtgaatgtt tgtcaccagt gcctccagca
     7921 ccactttcca gtcatcaacc acgccaacat ctgcctgtga aaataccgca gcgctggcgt
     7981 catgattaat cgccaccaca aacttgctat tgcgtacgcc cgccattaac gctgctgcgc
     8041 cagaagcgcc aacaacaata cacacctcag gtgccagcag atgtccggaa ataccaatca
     8101 ctttttcagc atcaacgcca ccgttcatca ctcgcgcccg actgtagccc acttcggctc
     8161 ccagcttttc tgccagcata gcgatttcct ggttatctgc ctctccgccc tgccccacca
     8221 ccagcacccg tctggcctca gctaatgggt cgcgagtaac atttttcaga tcctctgtgc
     8281 taaccagcca gtccggaagc gcacccggga caatgttcag ttgttgcatt ccagagggca
     8341 acgtggcgtt tttcgctgct cccgcctgac gcgccaggga aaggcatagc gggcgttttt
     8401 ctgtttgcag cgtcgccgtt agcgcatttc cccagtggga tttgcgcacg cttacggttg
     8461 gaatatctaa cgaagtcacc tggcagatgc ttgcaccatg taaacgccag gccagccggg
     8521 tcgacagttc atcgccaaac gttcccggcg gaaagagaac aacgtccgca ggcgttcgct
     8581 gccactgttc caccaacgca tccagcacct gttccgccac tacaggctga ggttcaatct
     8641 gccagtgcgc cagggtacaa ccgctgaaat cctgcgccgc cagccagctt gctattgcgg
     8701 cattctcctg atttattgtc acaattgcga tattcatggc tgcatccttt ggcgtaaata
     8761 gtcctgccat agcttttgag ctttttctgc taccgtttgc ccatcaatca gcgtcgctcc
     8821 gcgtcgttgc tctgctcttg ccagttgcag acactgcatc gctggcattt cggcagcaac
     8881 agttttacgg ataatttctg ctttccccgc agccattcgc tggcgcattc ccggtactgg
     8941 caaggccact tcaccgcact gacgcacggc aatcaccgca ggcaagcgaa cccggcaaca
     9001 acgcaaccca tgttcagtac gctgttcgag ggtgataaac agcgcgtcga gagtgaaacg
     9061 ctccacctgg gtgaagcagg gccagcccag catttccgcc agcaaaaatg gcgtttgccc
     9121 attctgccct tcgctgcttt ggcagccggt aattatcaga tccagcggat tctgatgctg
     9181 ccattcggca atatggcgag cgacaaattc cggcgcaaag cgcagatctg ccgccgtctc
     9241 cagcaataca gcttcctcaa acccaagagc catgagatag cgcagccagt gcaatgcccg
     9301 ttcatccccc atgctcaacg cggttaaaga catcggcgta ccgttttttc tctgcgccaa
     9361 cagcagcgcg gcagcggcct gttcatcagc accgagtaaa cttcgcagta gcgaaatatc
     9421 cggtccgctt ttaccctgag ccgccgcctg ccactctttt tccgccagca ttccggcatc
     9481 cggttcggct ttaaacgcta acagaatgtt cattcgcacc tcctacattt cccgccgcca
     9541 ccagcgggag ggctttagtt tctggtgccc atagccaggt cacaacaaaa tcaaccaaca
     9601 acactgtcgc caggagcaat aaggtgactt gcattcccca ctgcgccagc acccacggca
     9661 gcaggccagt acttaccgcc gcgccaagtc gactcatggc ggtggcaaaa ccgacgccca
     9721 gcgagcgaat gtcagtagga aaactttccg caggcaaaat gcccaccaga ttactgactg
     9781 ccgaaatggt ggtgctgaag agaacaaaaa gcagcagcgt taatgaactg ccggaaggca
     9841 aacaggccat cactaccagc gttgccgcca gcagcaaaaa acttcccagc aaaaatttgc
     9901 gatgtgccag caggtgcgtc agaactaatc ccagcagcgc gcccacaatt aacaacgcat
     9961 taagcatcag gctggcagtc agcgcatctt ccagaccaat agtctgggcg atagttggca
    10021 gccaggtata aatcacaaac catgggatta cgaggcagac aaagaagacg ctgttaaacg
    10081 ccgtgcgccg ccagtaacgc gaagagaaca aggttttgat gtgtttatgg gtcgccgtta
    10141 ccacttcatc gcccagtaaa acatggggac caaaatagcg atgcacgata gcgtgagctt
    10201 ctgcaaaacg cccctggcgc agtagccagc gtggtgattc tggcgttccc cagcgtaata
    10261 acgtaatcaa caacgcgggc agagctgccg atgccagtag ccagcgccag gcctccgggt
    10321 tttcggaaat aaagtgatgt ccggcaatac ttgccagcac atagcctacg gtccacacca
    10381 cgctgaatgc gcccagcaaa ataccgcgat ggcggcgcgg ggaaaattca gccagcaagg
    10441 tgtgacctac tgaataatcg cctcccagac caatgccaat caaaatgcgc agtccaataa
    10501 gatgctctgg cgtggtggca aaaaattgta aaaacgaagc aagcgtaatc agcaaaaagc
    10561 tgaaggtgaa gattttttgc cgaccaatat ggtcggagat ccacccaaga accaggctgc
    10621 caaggaacaa accaaggagc gccgagccgc cgatcattcc cgccataaac ggcgtcagtt
    10681 gcatggcggg cgtaagctga ataatggcgt aaccaatgac gccgagaaca taaccgtcgg
    10741 tcaggtgtgc gccgaaagtg agcgcagcaa tgcggcagtg aaaacggtta agcggtaaat
    10801 catccattcg caccggtgaa gtgttcatct ttaccccttc attgtccggt aataagaacg
    10861 gcttctcgcc tgagaagccg aaaaggcaac gccccgtcat cgcggaacgc tgcctgacac
    10921 attatttttc aatcggatag atagtacccg tattcataat gccattagga tcgaactgct
    10981 ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc cagtttgctc cagtgaacgc
    11041 gatgtttacc gataccgtgg tggtgcacca tcgaaccgcc gaggcgaatg gtttcttcac
    11101 agatgatctt gttgagcgga ttgtggtact tgtcgatttc ctcttccggc ttacagtcaa
    11161 cgacgttgta atcgtagacg aagtacatgt tggtgccgtt ctgatagcta tgagaggaat
    11221 gaccgcccag catggtgatg tcgtcggcgt gcgggaactc agtacgaata cggttaataa
    11281 cgctttcgta gatttcgtgg atgcagctcc agcagccgga cacttcagtg gtaaagccca
    11341 tgttgccggt tttgaggatc tgcacacgtt cggcagccac tttatccggt ccccagttca
    11401 ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg ctggcattgc gggtagcggg
    11461 caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat gcgagggtta ccttcagcca
    11521 taaagatcag cacgcatttt ccgtctgcaa aatgggtgaa gtgttgggtg ccatcttcag
    11581 cgtcatacaa acgagcgatc gacggacgat acccttccac catgacttca cgcaggatgt
    11641 tgaagtcggt tttcatgtct tccaggatat agccgtagaa gaggttgttt tccggggtga
    11701 atttaaagat tttcactgtt acttcagtga tatagcaaag tgcaccttcg ttaccgatga
    11761 tgatgtggcg aatgtccggg ccagccgcgc ggcgtggcac gtttttaatg cgtgtgacgg
    11821 tgccatcagc caatactgct tccagaccaa cgaccatatc ttcgattgcg ccgtagagtg
    11881 tggagaactg cccgatactg cgggttgcta ccaagccgcc catctgcgcc agcggctttg
    11941 actgcggaga atgccccgtg gtgtaacctt tttcacgcaa cgcattttcc agcacctcca
    12001 gcggaacccc acattgcgcc gtcgcctgca tattctcaat atcaatatta atgatttgat
    12061 tcatggcgga gccgtcaagc accaccgagt tttccacaac ggtttccagc ccaccttcag
    12121 tggcggaagc gccggtacgc ggcacaccgt taattttgtg cgcattcata aaattcagca
    12181 cacgggatac ttgctctgtg gaaccgagtt ttacgaccgc tgccggaatc ggcaaagtat
    12241 aaatgccatg aatatccgga aatttacgaa aacggtcaat actgttcttc tttaatactg
    12301 tttcatcggt aattacgcga tctgcaccaa caatttcctt tagttggtcg acaatcgctg
    12361 cgcgagataa agacatagta attccttcct gataataaaa atggaataat cattaataag
    12421 agaaaacaga aaccaccgtt atggattagc gcactaaata accaccattg accactaata
    12481 aatgcccatt gacataattt gaagccggac ttgcgaggaa tacagctgcg cccattaaat
    12541 cctgagtatc gccccaacgg tttgccggaa tatgatcaag aacgcgctga ttggtttctg
    12601 gattactgcg tgtcgccagc gtaatatctg ttgcataata gccaggagca ataccattta
    12661 cctgaatatt atattgacct agttcatcac aataagcttt ggtgaatccg gcaagagcat
    12721 gtttagtggc agaatatgca ggtgaccatt gtccacctaa gtaagagaac aatgaacaga
    12781 tattaataat tttgccgctt ttctgcggga tcataatttt tgcagcttca tagcttaact
    12841 cgaatgcggc ggtcaggttc acatcaatca tcggatccca gtcggcacga ccgaagtcca
    12901 gcaccttatt cagcttacaa ataccggcat tgtttaccag aatatcaact gtaccgaaac
    12961 gctcacagca agcagcgata atcttctgcg gcgcgccttc tgcggtgata tccacctgca
    13021 tgaagtccac ctcaacaccc tgtttttcaa tcatttcctt tgtttcgccg ttatctttca
    13081 atcatttcct ttgtttcgcc gttatctttg acgaaactag gaataaagat atttgcgcca
    13141 gctttggcca acgccatggc aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt
    13201 gcggttttac ctttcagtga gaaaaaatcc attgagaacg cattgagaga ttcgattgac
    13261 atagttagct ccaatttccg taaggcaaaa aaaagaaagg taacttcccc cgcgagaggg
    13321 aagttacctt tctcattatc tctggtaact ggagataatt tttagcataa cggcataact
    13381 gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga
    13441 agcgtgatta ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta
    13501 gagacctgag aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta
    13561 ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat
    13621 aattagtttt agcggcggcg ttagttttga cctggcttat ttacgttata tttttcaaat
    13681 tcccatggcg aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt
    13741 tggtattgcg gctattattc tttatgcccc cagcggcgtt attgccgata aattttcaca
    13801 ccgcaaaatg attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac
    13861 gtatccaccg ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt
    13921 aatgctgtgg tcggtgtcga ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg
    13981 gaaaattatg ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt
    14041 taccatgtgg gtcttttctc gctttgcacc ggatgacagc gccagcctga aaacagtcat
    14101 tatcatctac agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga
    14161 taacaacaac ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat
    14221 ccttgccgtt ttgcgtatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt
    14281 cacaatctac gccattctga gttactccac caactatctg accgaaatgt acggcatgtc
    14341 gctggtggca gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg
    14401 cccacttgga ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtgattca
    14461 aatcctttcc atcatcggtt tgctggcgtt gacagccctg ctcgtcacga actctaaccc
    14521 ccaatcggtc gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc
    14581 acgcgggctg tactgggcct gccccggcga agcgagaaca ccgtcttaca ttatgggcac
    14641 cacggtaggt atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat
    14701 cggccactgg caagacaccc tgcccgctgc agaagcctac cgcaatatgt ggctgatggg
    14761 catggcggcg ctcggcatgg tgattgtctt tacctttttg ctgttccaaa aaattcgtac
    14821 tgctgatagc gcccccgcaa tagctagcag caagtaagcc aggaaaatta caggagaaaa
    14881 atcatgtcga aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg
    14941 atgtacgatc tggaaggtaa tgtggtttgc gaaggtaaag gcttattaca gccgatgcac
    15001 acgcctgatg ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt
    15061 gcaggtcacg atttgatgag ccagtttgcc gggaataaag aagatattgt tggcatcggc
    15121 ctgggctcca tccgttgctg ccgtgcgtta ttgaaagccg acggcacgcc agctgcgccg
    15181 ttgattagct ggcaggatgc acgcgttaca cgcccttacg aacatacgaa tcctgacgtg
    15241 gcgtatgtaa cctctttttc gggttatctg acgcatcgct taaccggcga atttaaagat
    15301 aatatcgcca actattttgg tcagtggccg gtggattata agacctgggc atggagcgaa
    15361 gatgccgcag tcatggagaa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg
    15421 ccaggcactg ttcttgggca tatcacacct caagccgcgc tggcgacgca tttcccggca
    15481 ggactgccgg ttgtttgtac caccagtgat aaaccggtag aagctctggg agccggatta
    15541 ctggatgatg aaactgcggt aatttctctt ggcacttaca tcgcattgat gatgaacggc
    15601 aaagccttgc cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca
    15661 ttgctgtatg aaggttacgg tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac
    15721 atgttaggcg agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta
    15781 ctcaacaaga aagcctctag tgtgccacct ggctgtaatg ggctgatgac ggtgctggac
    15841 tggctgacca atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc
    15901 atggattacg catggatata tcgttcgata ttggaaagcg tggcgctgac gctgaagaac
    15961 aattacgaca atatgtgtaa tgaaatgaat cactttgcga aacatgtaat cattactggc
    16021 ggcggttcga acaggatctg tttatgcaga tttttgccga cgtgttcaac cttccggcac
    16081 gccgtaacgc cattaacggt tgtgcaagcc tgggggcagc gattaataca ggggtaggtc
    16141 tggggctata cccggattac gcaacggctg tcgataaaat ggttcgcgtg aaagatatct
    16201 ttataccgat tgagagcaat gccaaacgct acgacgcgat gaataaaggc attttcaaag
    16261 acctaaccaa acatactgat gtgatcctga aaaaatcgta tgaagtgatg catggggaat
    16321 tggggaattg gggaatgcgg attcgatcca gagctggtcg aatgcgtgaa taaggtaggc
    16381 cgggcggaaa actgcccggc ctgaacatac ctgaatggtt atccccgctg gcgcggggaa
    16441 cataagttcg acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga
    16501 acataataaa aactttaact agttcggttt atccctgttt ttacaaggaa
//
LOCUS       DAEEBB010000093        16296 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEEBB010000093 DAEEBB010000000
VERSION     DAEEBB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.093"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..578
                     /locus_tag="KLF07_002794"
     CDS             <1..578
                     /locus_tag="KLF07_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBD9832769.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="KLF07_002795"
     CDS             581..1744
                     /locus_tag="KLF07_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD9832770.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="KLF07_002796"
     CDS             1828..3453
                     /locus_tag="KLF07_002796"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBD9832771.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="KLF07_002797"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="KLF07_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBD9832772.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="KLF07_002798"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="KLF07_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBD9832773.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="KLF07_002799"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="KLF07_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD9832774.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="KLF07_002800"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="KLF07_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBD9832775.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="KLF07_002801"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="KLF07_002801"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBD9832776.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="KLF07_002802"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="KLF07_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="KLF07_002803"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="KLF07_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBD9832777.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="KLF07_002804"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="KLF07_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBD9832778.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="KLF07_002805"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="KLF07_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBD9832779.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="KLF07_002806"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="KLF07_002806"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBD9832780.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="KLF07_002807"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="KLF07_002807"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBD9832781.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="KLF07_002808"
     CDS             12037..12588
                     /locus_tag="KLF07_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HBD9832782.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="KLF07_002809"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="KLF07_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="KLF07_002810"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="KLF07_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBD9832783.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="KLF07_002811"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="KLF07_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBD9832784.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="KLF07_002812"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="KLF07_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBD9832785.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="KLF07_002813"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="KLF07_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBD9832786.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="KLF07_002814"
     CDS             complement(14856..15827)
                     /locus_tag="KLF07_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBD9832787.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..>16296)
                     /locus_tag="KLF07_002815"
     CDS             complement(15880..>16296)
                     /locus_tag="KLF07_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBD9832788.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4155 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatga
//
LOCUS       DAEEBB010000094        15961 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEEBB010000094 DAEEBB010000000
VERSION     DAEEBB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.094"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="KLF07_002816"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="KLF07_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBD9832789.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="KLF07_002817"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="KLF07_002817"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBD9832790.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="KLF07_002818"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="KLF07_002818"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBD9832791.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="KLF07_002819"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="KLF07_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="KLF07_002820"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="KLF07_002820"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBD9832792.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="KLF07_002821"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="KLF07_002821"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBD9832793.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="KLF07_002822"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="KLF07_002822"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBD9832794.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="KLF07_002823"
     CDS             8229..8939
                     /locus_tag="KLF07_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD9832795.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="KLF07_002824"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="KLF07_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBD9832796.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="KLF07_002825"
     CDS             9636..10343
                     /locus_tag="KLF07_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD9832797.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="KLF07_002826"
     CDS             10343..10891
                     /locus_tag="KLF07_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBD9832798.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="KLF07_002827"
     CDS             10901..12067
                     /locus_tag="KLF07_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD9832799.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="KLF07_002828"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="KLF07_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="KLF07_002829"
     CDS             13576..14229
                     /locus_tag="KLF07_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD9832800.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="KLF07_002830"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="KLF07_002830"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBD9832801.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="KLF07_002831"
     CDS             complement(15612..>15961)
                     /locus_tag="KLF07_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD9832802.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3700 a         4345 c         4162 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAEEBB010000095        15926 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEEBB010000095 DAEEBB010000000
VERSION     DAEEBB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.095"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..168
                     /locus_tag="KLF07_002832"
     CDS             <1..168
                     /locus_tag="KLF07_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD9832803.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="KLF07_002833"
     CDS             complement(340..669)
                     /locus_tag="KLF07_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBD9832804.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="KLF07_002834"
     CDS             complement(674..859)
                     /locus_tag="KLF07_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBD9832805.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="KLF07_002835"
     CDS             complement(856..3495)
                     /locus_tag="KLF07_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBD9832806.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="KLF07_002836"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="KLF07_002836"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD9832807.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="KLF07_002837"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="KLF07_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBD9832808.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="KLF07_002838"
     CDS             complement(5222..5488)
                     /locus_tag="KLF07_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBD9832809.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="KLF07_002839"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="KLF07_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBD9832810.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9652..10785
                     /gene="ompN"
                     /locus_tag="KLF07_002840"
     CDS             9652..10785
                     /gene="ompN"
                     /locus_tag="KLF07_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBD9832811.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10926..11360
                     /gene="uspF"
                     /locus_tag="KLF07_002841"
     CDS             10926..11360
                     /gene="uspF"
                     /locus_tag="KLF07_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBD9832812.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11946..12860
                     /gene="sitA"
                     /locus_tag="KLF07_002842"
     CDS             11946..12860
                     /gene="sitA"
                     /locus_tag="KLF07_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBD9832813.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12860..13687
                     /gene="sitB"
                     /locus_tag="KLF07_002843"
     CDS             12860..13687
                     /gene="sitB"
                     /locus_tag="KLF07_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBD9832814.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13684..14541
                     /gene="sitC"
                     /locus_tag="KLF07_002844"
     CDS             13684..14541
                     /gene="sitC"
                     /locus_tag="KLF07_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBD9832815.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14538..15395
                     /gene="sitD"
                     /locus_tag="KLF07_002845"
     CDS             14538..15395
                     /gene="sitD"
                     /locus_tag="KLF07_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBD9832816.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15596..>15926)
                     /locus_tag="KLF07_002846"
     CDS             complement(15596..>15926)
                     /locus_tag="KLF07_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD9832817.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3984 a         4022 c         3829 g         4091 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata
     9421 taacccgcgc agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta
     9481 tcttcgatac attcaccaga cttattatca tttcagaaga attatttgat taaggtttta
     9541 cttaaggcgt aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca
     9601 ctccccggaa tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc
     9661 aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa
     9721 gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat
     9781 ttttctgata attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc
     9841 gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca
     9901 aacaacactg aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa
     9961 tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa
    10021 ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt
    10081 atgactggtc gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta
    10141 aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag
    10201 gaaggcacca acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc
    10261 acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc
    10321 accaatgacc aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct
    10381 gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat
    10441 atgaccccgt ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc
    10501 actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa
    10561 ggccgtgacc tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat
    10621 cttgttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat
    10681 gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc
    10741 tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct
    10801 gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact
    10861 ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag
    10921 gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac
    10981 gcgtgattag ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga
    11041 cggtaatacc ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac
    11101 cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat
    11161 ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca
    11221 ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg
    11281 atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct
    11341 ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt
    11401 tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc
    11461 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
    11521 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
    11581 acaagtaagg gcaaaaatca caactatctg acccataagt attttattta tttttcaata
    11641 tgttaaacat tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt
    11701 tttgcccctt ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc
    11761 atttcaaagc ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac
    11821 aacctaatcc ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg
    11881 gttatgctat aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact
    11941 aaattatgca ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct
    12001 cgatcgcatt tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca
    12061 tcgcggatat ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc
    12121 ctggtgcaga aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg
    12181 cacaactgat tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc
    12241 atctcaatgg ggttccagaa gtaattgtct cttcgggtgt gacgccagta gggatcaccg
    12301 aaggacccta tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga
    12361 tttacgtcga taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct
    12421 accaacgtaa tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta
    12481 agcagattac ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt
    12541 cttatctcgc acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc
    12601 aacaaggaac accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc
    12661 cggcagtctt tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa
    12721 ccggcgcaca ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg
    12781 tacctacgta tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag
    12841 ccgggaaaag ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt
    12901 cacctggcgt aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc
    12961 tattgccgct ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat
    13021 gggatttgtg cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc
    13081 gctacagaaa aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc
    13141 tgtactggtg gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat
    13201 agccaaaaag agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga
    13261 ttttcgccat cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc
    13321 gagagcgatt gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga
    13381 tgtgaaaacc gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac
    13441 gatgcttgtc tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat
    13501 ggtcaaaggc accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct
    13561 ggagctggct tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat
    13621 tattactgac gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc
    13681 aagatgaacg tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc
    13741 tcggcaatgg tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg
    13801 tcgctgattg gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg
    13861 ctggggcttc cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg
    13921 ctgtttctta accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg
    13981 tccttttttg gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag
    14041 accatcgtcc tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc
    14101 attggcattc tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt
    14161 ttcgacgaga atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc
    14221 ttcaccctgc tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg
    14281 atctgtctgg tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc
    14341 ctgctcataa ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt
    14401 tactttctgg acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt
    14461 ttagcatttg tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa
    14521 gcgctggagg atcgctcatg atggcattat tactggagcc actgcagttc acctttatga
    14581 gtcacgcact gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc
    14641 tggtcctgaa aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca
    14701 tcgtgcttac ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat
    14761 tctgcgcggt ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta
    14821 tggggattgt tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac
    14881 cagacgtaca tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata
    14941 taatccagac ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg
    15001 attttttgct gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat
    15061 ggctgcatta tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag
    15121 taggtatcat tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga
    15181 cacaacgctt tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga
    15241 ctggtgtctg gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat
    15301 tcgcggtcgt gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa
    15361 acgcccatac acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc
    15421 cgccagccta cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta
    15481 tcatgcaaaa aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg
    15541 aaagtttacc agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt
    15601 ccacgataaa attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct
    15661 cccgctgacg gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg
    15721 ccagttcttc cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg
    15781 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
    15841 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
    15901 cctgttctgt gcgccatgct tcaatt
//
LOCUS       DAEEBB010000096        15694 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEEBB010000096 DAEEBB010000000
VERSION     DAEEBB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.096"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..1453
                     /locus_tag="KLF07_002847"
                     /pseudo
     CDS             1..1453
                     /locus_tag="KLF07_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="KLF07_002848"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="KLF07_002848"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD9832818.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="KLF07_002849"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="KLF07_002849"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBD9832819.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="KLF07_002850"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="KLF07_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBD9832820.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="KLF07_002851"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="KLF07_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBD9832821.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="KLF07_002852"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="KLF07_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBD9832822.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="KLF07_002853"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="KLF07_002853"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBD9832823.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="KLF07_002854"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="KLF07_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBD9832824.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="KLF07_002855"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="KLF07_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBD9832825.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="KLF07_002856"
     CDS             8572..9174
                     /locus_tag="KLF07_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBD9832826.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="KLF07_002857"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="KLF07_002857"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBD9832827.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="KLF07_002858"
     CDS             complement(9732..10472)
                     /locus_tag="KLF07_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD9832828.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="KLF07_002859"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="KLF07_002859"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBD9832829.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="KLF07_002860"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="KLF07_002860"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBD9832830.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="KLF07_002861"
     CDS             13153..13719
                     /locus_tag="KLF07_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD9832831.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="KLF07_002862"
     CDS             14074..14322
                     /locus_tag="KLF07_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD9832832.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15694
                     /locus_tag="KLF07_002863"
     CDS             14923..>15694
                     /locus_tag="KLF07_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD9832833.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT         3985 a         3974 c         3603 g         4132 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catg
//
LOCUS       DAEEBB010000097        15582 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEEBB010000097 DAEEBB010000000
VERSION     DAEEBB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.097"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..2065
                     /locus_tag="KLF07_002864"
     CDS             <1..2065
                     /locus_tag="KLF07_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD9832834.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="KLF07_002865"
     CDS             2081..3115
                     /locus_tag="KLF07_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBD9832835.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="KLF07_002866"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="KLF07_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBD9832836.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="KLF07_002867"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="KLF07_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBD9832837.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="KLF07_002868"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="KLF07_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="KLF07_002869"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="KLF07_002869"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBD9832838.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="KLF07_002870"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="KLF07_002870"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBD9832839.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="KLF07_002871"
     CDS             6844..7662
                     /locus_tag="KLF07_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBD9832840.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="KLF07_002872"
     CDS             7714..8460
                     /locus_tag="KLF07_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD9832841.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="KLF07_002873"
     CDS             complement(8434..9399)
                     /locus_tag="KLF07_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBD9832842.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="KLF07_002874"
     CDS             complement(9396..10400)
                     /locus_tag="KLF07_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBD9832843.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="KLF07_002875"
     CDS             complement(10397..11674)
                     /locus_tag="KLF07_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9832844.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="KLF07_002876"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="KLF07_002876"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBD9832845.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="KLF07_002877"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="KLF07_002877"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBD9832846.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="KLF07_002878"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="KLF07_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="KLF07_002879"
     CDS             15452..>15582
                     /locus_tag="KLF07_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBD9832847.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAEEBB010000098        15406 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEEBB010000098 DAEEBB010000000
VERSION     DAEEBB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.098"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            10..453
                     /locus_tag="KLF07_002880"
     CDS             10..453
                     /locus_tag="KLF07_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD9832848.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="KLF07_002881"
                     /pseudo
     CDS             669..758
                     /locus_tag="KLF07_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="KLF07_002882"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="KLF07_002882"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBD9832849.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="KLF07_002883"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="KLF07_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBD9832850.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="KLF07_002884"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="KLF07_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9832851.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="KLF07_002885"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="KLF07_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBD9832852.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="KLF07_002886"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="KLF07_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBD9832853.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="KLF07_002887"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="KLF07_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBD9832854.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="KLF07_002888"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="KLF07_002888"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBD9832855.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="KLF07_002889"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="KLF07_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBD9832856.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="KLF07_002890"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="KLF07_002890"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBD9832857.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="KLF07_002891"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="KLF07_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBD9832858.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="KLF07_002892"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="KLF07_002892"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBD9832859.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="KLF07_002893"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="KLF07_002893"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBD9832860.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="KLF07_002894"
     CDS             13055..13435
                     /locus_tag="KLF07_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBD9832861.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="KLF07_002895"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="KLF07_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBD9832862.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="KLF07_002896"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="KLF07_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBD9832863.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="KLF07_002897"
     CDS             complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="KLF07_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBD9832864.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4043 c         3851 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt taaaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaatg taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAEEBB010000099        15238 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEEBB010000099 DAEEBB010000000
VERSION     DAEEBB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.099"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..752
                     /locus_tag="KLF07_002898"
     CDS             <1..752
                     /locus_tag="KLF07_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HBD9832865.1"
                     /translation="ESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYE
                     LDKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQE
                     VNAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLL
                     NFDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTAS
                     LQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(793..1725)
                     /locus_tag="KLF07_002899"
     CDS             complement(793..1725)
                     /locus_tag="KLF07_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD9832866.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2089..4875
                     /gene="polA"
                     /locus_tag="KLF07_002900"
     CDS             2089..4875
                     /gene="polA"
                     /locus_tag="KLF07_002900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBD9832867.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5257..5889)
                     /locus_tag="KLF07_002901"
     CDS             complement(5257..5889)
                     /locus_tag="KLF07_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBD9832868.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6471..6980
                     /gene="yihI"
                     /locus_tag="KLF07_002902"
     CDS             6471..6980
                     /gene="yihI"
                     /locus_tag="KLF07_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBD9832869.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7169..8542
                     /gene="hemN"
                     /locus_tag="KLF07_002903"
     CDS             7169..8542
                     /gene="hemN"
                     /locus_tag="KLF07_002903"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBD9832870.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8732..8842)
                     /locus_tag="KLF07_002904"
     CDS             complement(8732..8842)
                     /locus_tag="KLF07_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBD9832871.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8954..10363)
                     /gene="glnG"
                     /locus_tag="KLF07_002905"
     CDS             complement(8954..10363)
                     /gene="glnG"
                     /locus_tag="KLF07_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBD9832872.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10375..11424)
                     /gene="glnL"
                     /locus_tag="KLF07_002906"
     CDS             complement(10375..11424)
                     /gene="glnL"
                     /locus_tag="KLF07_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBD9832873.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11598..13007)
                     /gene="glnA"
                     /locus_tag="KLF07_002907"
     CDS             complement(11598..13007)
                     /gene="glnA"
                     /locus_tag="KLF07_002907"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBD9832874.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13380..15203
                     /gene="typA"
                     /locus_tag="KLF07_002908"
     CDS             13380..15203
                     /gene="typA"
                     /locus_tag="KLF07_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBD9832875.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3804 c         3916 g         3553 t
ORIGIN      
        1 gcgagtcgaa atttaatcag attaacatta tcaatcacgg ggaagacgtt gcccaaatcg
       61 atgctttcgt tgcaaaaacc atgctggatc gcgttaaaga caaagattat atcaatgtca
      121 atctgaccta cgaacttgat aagttaacaa aagggaatca gcaactcggt agtggtgagt
      181 ggtcattgat tgctgaatct attgatccct cagcagtgcg ccaatttatc atccagtata
      241 acattgctat gcagaagcag cttgctgtac accctgagtt agcaaacgat gaagttgctc
      301 tgcaagaagt gaatgctgca ttgttcaaag agtatttacc gttattacaa aaaagtgagc
      361 cgaccattaa acaaccggta agatggaaga acgcactcgg cgaactaaat gccaatctgg
      421 atatcagtat tgccgaccca gccaaatctt catcatccac aaacaaagat atcaaattgc
      481 tcaattttga tgtgaagtta ccgcttaatg tcgtcacaga aaccgcaaaa cagcttaatt
      541 tatctgaagg aatggatgcg gaaaaagcgc aaaagcaggc tgataaacaa atcagcggga
      601 tgatgacatt gggtcagatg tttcagttaa tcacgattga caacaatacc gcctcgctgc
      661 agctgcgtta tacaccgggt aaagttgtgt ttaacggaca ggagatgagc gaagaagaat
      721 ttatgtctcg tgccggacgt tttgttcatt aaaatactca ctcgcctctg ctcattcaga
      781 ggcgagtaaa acttacttat tctgacgctg tgatgacatg agagacgtca gaagccggtc
      841 tttttcctgc cacaatgagt tcagccattg ctgaaaatgg cgcttaaagc ttttgtcatt
      901 gatgtagtcg ccatgtaact catcggcaat aggctgtaaa tccacatgta cgacaatccg
      961 cgtcagttta ccgcttaaca tatcgaagaa cggctgacgg ttattgtccg gataacacag
     1021 cgtaacatta agcagtttat cgaattgttt acccagtaca tttagcgcca tcgcaatgcc
     1081 tgccgccttt ggtggcaaca agttttgaaa agtggagtgg gtttgctgat gtttttcttg
     1141 cgtgaaacgg gagccttcaa cgaaattgac aatggtggtg ggatgcaggc gaaacttttc
     1201 acaagaacgc cgagtggttt caacatcttt accacgtcgc tccggatgac gtaataaata
     1261 agcgcgggaa taacgcttca taaacggcat atccagcgcc cagcacgcca ggccaagaaa
     1321 tggcacccag gccagctgtt gcttgaggaa atatttattc atcggaatgt gcttacgaaa
     1381 cagcacgcac agtacgacaa tatctgccca actacggtgg ttacaaataa gcagatacca
     1441 gttcttctta cttaaccctt ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg
     1501 cagtaatacc gccagacctt cacaccagca atacatcata aaatcacaaa aacgcgaaac
     1561 ctttcgccaa atgactggca caggtagcaa tagttttaca atcccggcaa tgatgatcgg
     1621 gacggaacaa aaaatagtga ccaaaatggt caatacgata ctcagcaaaa gggttatcgc
     1681 agcgagtatt ctcgtcataa tgaatttatt caaaaggtta gccatagaca gtgcgctaaa
     1741 gaagcgcaag gcgcagattt tagcagaaaa cgacccaaat aacggatgat ccttaaggag
     1801 aaaaataatc catatctatc cacattagaa aaaatcccat tatctcaatt attagggatg
     1861 gctttatttt taactgcatg aaaaacaaag acaaacatca tgctgtaaaa agcatgataa
     1921 taaattaaaa gcgatgtaaa taatttatgc acaaagttat ccacatgacg atttgcgagc
     1981 gatccagaag atctacaaaa gattttcacg aaaagcggtg aaaaactcat gttttcatcc
     2041 tgtctgtggc atcctttacc cataatctga taaacaggca cggacattat ggttcagatc
     2101 ccccaaaatc cacttatcct tgtagatggt tcatcttatc tttatcgcgc atatcacgcg
     2161 tttcccccgc tgactaacag cgcaggcgag ccgaccggtg cgatgtatgg tgtcctcaac
     2221 atgctgcgca gtctgatcat gcaatataaa ccgacgcatg cagcggtggt ctttgacgcc
     2281 aagggaaaaa cctttcgtga tgaactgttt gaacattaca aatcacatcg cccgccaatg
     2341 ccggacgatc tgcgagcaca aatcgaaccc ctgcacgcga tggttaaagc gatgggactg
     2401 ccgctgctgg cggtttctgg cgtagaagcg gacgacgtta tcggtactct ggcgcgcgaa
     2461 gccgaaaaag ccgggcgtcc ggtgctgatc agcactggcg ataaagatat ggcgcagctg
     2521 gtgacgccaa atattacgct tatcaacacc atgacgaata ccatcctcgg accggaagag
     2581 gtggtgaata agtacggcgt gccgccagaa ctgatcatcg atttcctggc gctgatgggt
     2641 gactcctccg ataacattcc tggcgtaccg ggcgtcggtg aaaaaaccgc gcaggcattg
     2701 ctgcaaggtc ttggcgggct ggatacgctg tatgccgagc cagaaaaaat tgctgggttg
     2761 agcttccgtg gcgcgaaaac aatggcagcg aagctcgagc aaaacaaaga agttgcttat
     2821 ctctcatacc agctggcgac gattaaaacc gacgttgaac tggagctgac ctgtgaacaa
     2881 ctggaagtgc agcaaccggc agcggaagag ttgttggggc tgttcaaaaa gtatgagttc
     2941 aaacgctgga ctgctgatgt cgaagcgggc aaatggttac aggccaaagg ggcaaaacca
     3001 gccgcgaagc cgcaggaaac cagtgttgca gacgaagcgc cagaagtgac ggcaacggtg
     3061 atttcttatg acaactacgt caccatcctt gatgaagaaa cactgaaagc gtggattgcg
     3121 aagctggaaa aagcgccggt atttgcattt gataccgaaa ccgacagcct tgataacatc
     3181 tctgctaacc tggtcgggct ttcttttgct atcgagccag gcgtagcggc atatattccg
     3241 gttgctcatg attatcttga tgcgcccgat caaatctctc gcgagcgtgc actcgagttg
     3301 ctaaaaccgc tgctggaaga tgaaaaggcg ctgaaggtcg ggcaaaacct gaaatacgat
     3361 cgcggtattc tggcgaatta cggcattgag ctgcgtggga ttgcgtttga taccatgctg
     3421 gagtcctaca ttctcaatag cgttgccggg cgtcacgata tggacagcct cgcggaacgt
     3481 tggttgaagc acaaaactat cacttttgaa gagattgcgg gtaaaggcaa aaatcaactg
     3541 acctttaacc agattgccct cgaagaggcc ggacgttacg ccgccgaaga tgcggatgtc
     3601 accttgcagt tgcatctgaa aatgtggcca gatctgcaaa aacacaaagg gccgttgaac
     3661 gtcttcgaga atatcgaaat gccgctggtg ccggtgcttt cacgcattga acgtaacggt
     3721 gtgaagatcg atccgaaagt gctgcacaat cattctgaag agctcaccct tcgtctggct
     3781 gagctggaaa agaaagcgca tgaaattgca ggtgaggaat ttaacctttc ttccaccaag
     3841 cagttacaaa ccattctgtt tgaaaaacag ggcattaaac cgctgaagaa aacgccgggt
     3901 ggcgcgccgt caacgtcgga agaggtactg gaagaactgg cgctggacta tccgttgcca
     3961 aaagtgattc tggagtatcg tggtctggcg aagctgaaat cgacctacac cgacaagctg
     4021 ccgctgatga tcaacccgaa aaccgggcgt gtgcatacct cttatcacca ggcagtaact
     4081 gcaacgggac gtttatcgtc aaccgatcct aacctgcaaa acattccggt gcgtaacgaa
     4141 gaaggtcgtc gtatccgcca ggcgtttatt gcgccagagg attatgtgat tgtctcagcg
     4201 gactactcgc agattgaact gcgcattatg gcgcatcttt cgcgtgacaa aggcttgctg
     4261 accgcattcg cggaaggaaa agatatccac cgggcaacgg cggcagaagt gtttggtttg
     4321 ccactggaaa ccgtcaccag cgagcaacgc cgtagcgcga aagcgatcaa ctttggtctg
     4381 atttatggca tgagtgcttt cggtctggcg cggcaattga acattccacg taaagaagcg
     4441 cagaagtaca tggaccttta cttcgaacgc taccctggcg tgctggagta tatggaacgc
     4501 acccgtgctc aggcgaaaga gcagggctac gttgaaacgc tggacggacg ccgtctgtat
     4561 ctgccggata tcaaatccag caatggtgct cgtcgtgcag cggctgaacg tgcagccatt
     4621 aacgcgccaa tgcagggaac cgccgccgac attatcaaac gggcgatgat tgccgttgat
     4681 gcgtggttac aggctgagca accgcgtgta cgtatgatca tgcaggtaca cgatgaactg
     4741 gtatttgaag ttcataaaga tgatgttgat gccgtcgcga agcagattca tcaactgatg
     4801 gaaaactgta cccgtctgga tgtgccgttg ctggtggaag tggggagtgg cgaaaactgg
     4861 gatcaggcgc actaagattc gcctgaacat gccctttttt cgtaagtaag caacataagc
     4921 tgtcacgttt tgtgatggct attagaaatt cctatgcaac aactgaaaaa aaattacaaa
     4981 aagtgctttc tgaactgaac aaaaaagagt aaagttagtc gcgtagggta cagaggtaag
     5041 atgttctatc tttcagacct tttacttcac gtaatcggat ttggctgaat attttagccg
     5101 ccccagtcag taatgactgg ggcgtttttt attgggcgaa agaaaagatc cgtaatgcct
     5161 gatgcgctat gtttatcagg ccaacggtag aattgtaatc tattgaattt acgggccgga
     5221 tacgccacat ccggcacaag cattaaggca agaaaattat tcgccgtcct gcgtttcttc
     5281 tacaggctgc atctcgctaa accaggtatc cagtttctgc cgcagcttgt ccacgccttg
     5341 tttcttcaac gaagaaaacg tttcaacctg cacatcaccg ttaaacgcca gtacagcttc
     5401 acgcaccata ttcaattgcg ctttacgtgc gccgcttgcc agtttgtccg ctttggtcag
     5461 cagcaccaga acggcgatat tgctgtctac cgcccactca atcatctgct gatccaaatc
     5521 tttcagcgga tggcgaatat ccattagcac caccagacct tgcaggctct gacgtttttc
     5581 gaggtattcg ccgagcgcac gctgccattt gcgcttcatc tcttccggga cttccgcata
     5641 accgtaccca ggcaagtcaa ccagacgctt gccgtcagcc acttcaaaca ggttgataag
     5701 ctgggtgcgc cctggggttt ttgaggtacg agccaggctt ttctggttag tcagcgtgtt
     5761 cagcgcgctg gatttacctg cgttggaacg gcctgcaaaa gccacttcaa ttccggtatc
     5821 ggaaggtagg tggcgaatat caggcgcact catcacaaaa tgcgtctgtt gataattcaa
     5881 attagtcaaa gcggtcgtct ccgtcagtca aagctttgtg gcgattatac ctgaaccata
     5941 ataaaaggcg gatttttcgg cgtgagcgtt gtaagtaaaa gccatacgct ttgtgagaca
     6001 ttgccgatag tctttatgcg aaatagcaga gaaaattctg cgatgcatgt caaataagct
     6061 atataaatca gtgaattgct tttatataaa actctgaaaa tgtgcaaata ctgatggcgg
     6121 ttgattgttt gtttaaagca aaggcgtaaa gtagcaccca tagagcgagg acgctaacag
     6181 gaacaatgac tcaggatgag ggtcaggagc gccaggaggc gaagacagag gattgtcagg
     6241 aagacaaacg tccggagacg taattaaacg gaaatggaat caacacggat tgttccggct
     6301 aaaggaaaaa cagggtgtgt tggcggcctg caaggattgt aagacccgtt aagggttatg
     6361 agtcaggaaa aaaggcgaca gagtaatctg tcgccttttt tctttgcttg ctttctgtta
     6421 gattccgccg caaatctata ctgaataaaa cggctaaaag acgaaccatt atgaaaccat
     6481 catcttcaaa ctcacgcagc aaaggtcatg caaaagcgcg tcgaaaaaca cgcgaggagc
     6541 tggatcagga agctcgtgat cgcaagcgtc agaaaaaacg tcgtggtcat gcgccgggca
     6601 gccgcgcagc gggcggaaac accacgtcag gcagcaaagg ccagaacgca ccaaaagatc
     6661 cacgtattgg cagtaaaacc cctattccat tgggcgtggc tgaaaaagtc accaaacagc
     6721 acaaaccgaa gagtgagaaa cctatgcttt caccgcaggc ggagttggaa ttactggaaa
     6781 cggatgagcg tctggatgcg ctgctggaac gtctggaagc aggcgaaacc ctgagcgccg
     6841 aagagcaatc ctgggtggat gctaaactgg atcgtattga tgagttgatg cagaaactcg
     6901 gcctctctta tgacgatgac gaagaagagg aagaagacga gaagcaagaa gacatgatgc
     6961 gtctgctgcg gggtaactaa cggattgcca ccgtgggcct tcccgttctg ctgataaccc
     7021 ttccggttat atgttatctg gtgtggttat tcgttaaact acagcggttg tcgcggcgac
     7081 aaaagtggct gcgcaaccgg ctgatgaccc gaaacgggca tccgccggta cgccgtagcc
     7141 gccagagacg ccatcggaag gagtgagcat gtctgtacag caaatcgact gggatctggc
     7201 cctgatccag aaatataact attccgggcc acgatacacc tcgtacccga ccgcgctgga
     7261 gttttcagaa gacttcggcg aacaggcgtt tttacaagcc gtggcgcgct atcctgagcg
     7321 tccattatct ctctacgtac atatcccgtt ctgccataag ctttgttact tctgcggttg
     7381 caataagatt gttactcgcc agcagcacaa ggccgatcag tatctggacg cgctggagca
     7441 agaaatcgtc catcgtgcac cgctgttcgc cgggcgtcac gtcagccaat tgcactgggg
     7501 cggcggaacg ccgacgtatc tgaataaagc gcaaatcagc cgcctgatga agctgctgcg
     7561 cgaaaacttc cagttcaatg ccgatgcgga gatttcgatc gaagtcgatc cgcgggaaat
     7621 cgaactggat gtactcgatc atttacgcgc cgaaggcttt aatcgcctga gcatgggcgt
     7681 gcaggacttc aacaaagaag tgcaacgtct ggttaaccgc gagcaggatg aagagttcat
     7741 ctttgcactg cttaaccatg cgcgtgagat tggttttacc tccaccaaca tcgacctgat
     7801 ttacggcctg ccgaaacaga cgccggagag tttcgccttt accctgaaac gtgtggcgga
     7861 gctgaacccc gatcgtctga gtgtctttaa ctacgcgcat ctgccgacca tttttgctgc
     7921 tcagcgcaaa atcaaagatg ctgacctgcc gagtccgcag caaaaactcg atatcctgca
     7981 ggaaaccatc gccttcctga cgcaatcggg ctatcagttt atcggtatgg atcactttgc
     8041 ccgtccggat gacgagctgg cggtggccca gcgtgaaggc gtgctgcatc gtaacttcca
     8101 gggctacacc actcagggcg ataccgatct gctggggatg ggcgtttccg ccatcagcat
     8161 gattggcgac tgctacgcgc agaaccagaa agagttgaag cagtactatc agcaagtgga
     8221 tgaacaaggc aatgctctgt ggcgtggtat tgcgctaacg cgtgatgact gtattcgccg
     8281 cgatgtgatt aagtcgctca tctgcaactt ccgtctggat tacgccccta ttgagaaaca
     8341 gtgggatttg cacttcgctg attactttgc ggaagatctc aagctgctcg ccccgttagc
     8401 aaaagatggg ctggtggatg tggatgagaa gggaatacag gtgacggcga aaggtcgctt
     8461 gctgatccgc aacatttgca tgtgctttga tacctatctg cgccagaaag cgcggatgca
     8521 gcagttctca cgggtgattt aaatagtgaa tggcgcttcg tttacaaagt agaaaacgaa
     8581 agcgccatca atgtgctgag aacaagattg cctgatgcgc tacgcttatc aggcctacaa
     8641 aacctgttga aaatattgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
     8701 aacaaagagc acttcatcag caatctgaaa ctcaactaaa cagcccaatc attgcggcac
     8761 acagcacggc agcaacggca gtttgcggtg tgtggctgtc aactgcgcca ctcgatacca
     8821 gattaattat tgattccagc ataatgactc tccccgtttt ccgggcaaga tcatactgaa
     8881 cttatcggaa cagtaaagcg taaaatacca gcaatttgcg ctcaataatc aatctttaca
     8941 cacaagctgt gaatcactcc atccccagct cttttaactt acgcgtcagg gtgttgcggc
     9001 cccagccgag tagccgcgcc gcttcctgtt tatgcccctg cgtatgtcgt aacgcggtcg
     9061 tcagtaacgt ccgctccagc tctggctgcg cttcggaaag cagattttga tgaccggaac
     9121 gcagcgctct gtctgcccac tgcgcaagaa gcgttgccca gctgtccggt tgcatttgcg
     9181 aagtactctc cgcaaccgtt gattcaaaca gttcgccggg caaatcctga atcaacactt
     9241 cctgcccggc ggccatcacc gttagccagc ggcaggtgtt ttccagctgg cgcacgttgc
     9301 ctggccacgc cagacgcgtc agagcagctt cggtttccgg atgcagtaac ttcgcttcta
     9361 cgcccagttc gcgcgcggca acctgtaaaa aatggcgcgc cagacgggga atatcttccc
     9421 gacgttcgcg cagcggcggc agatgaacgc ggataacgtt caggcggtgg aacagatcct
     9481 cacggaactt accttcctgc actcgctgtt cgagattctg gtgagtggca gcgataatac
     9541 gcacatccac tttcaccggc gcatagccgc caacgcggta aaactgaccg tctgccagca
     9601 cgcgcagcaa acgcgtctgc acatccagcg gcatatcacc aatttcgtcg aggaataatg
     9661 taccgccatc ggcctgttca aaacgcccct gacgaatggt attcgcgcca gtaaacgcgc
     9721 ctttctcgtg gccaaacagt tctgattcga tcaaatcttt tgggatagct gccatattca
     9781 gcgcgataaa cggcgctttg gcgcgcggac tgtggcgatg cagggcatga gcgaccagtt
     9841 ctttaccggt gccggattcg ccgttaatca gcacgctaat agaagaacgc gaaagccgac
     9901 cgataatacg gaacacgtcc tgcatggctg gcgcttcgcc gatgatatcg gtcgttgggc
     9961 cgttaagctg aatattacgc ggctgctgct gttcctggta atgactgata gcgcgctcaa
    10021 ccagcgccac tgcttcgtcg atatcaaacg gtttgggcag ataatcaaac gccccttgtt
    10081 gataggcgct gacggcagca tccagatcgg aatgtgcggt cataatgatg accggaagca
    10141 ttggatggcg ctgtttaatc tgcttgagca gcgccagccc gtccattccc ggcatacgga
    10201 tatctgaaag cagcacatcc ggcgttttgc tcgccagcgc ctccagtact tccgcgccgt
    10261 tctcaaatgt cgtacaggtt aaacccgctc cagcgagcgc acgttcaagc acccaacgga
    10321 tggaactatc gtcatcgact acccagacta tccctcgttg cataaacgtc acctttattt
    10381 cctgataggc aggtaaaccg agaactcggt atgacctggc caactggtaa attcaatttt
    10441 gcctgaatgc tgatcaatca aattacgggc gatggataag ccaagcccgg tgccaccttc
    10501 gcggccgctg accatcgggt aaaacagcgt atcctgcaaa tgaggcggaa tgcccggccc
    10561 gttatcttcc acatcaatcc gcgccgccag ccggtagcgc tcgccgtgta aggtcagttg
    10621 aaacgcggtg cgggtacgca gaatgatttc accgccttcc ggccccagcg cctgtagcgc
    10681 attgcgcaca atattcagca agacctgttc aatttgatcc gggtcgtgcg ccagttccgg
    10741 caggctgggg tcgtaatcac gaatcaaccg cacgttgtcc ggcagttcca tcgacaccag
    10801 cgtcaccacg cgttcagcta ctttgtgaat actttcggta acgcgcgtac cgggcagctg
    10861 cggccccaac agacggtcga ccagatttcg cagccggtcc gcctgttcga taatcacttt
    10921 ggtatattcg agtagtgatg ggtcaggtaa cgctttgctg agcagctgcg ccgcgccacg
    10981 taaacctcca agcggatttt taatctcatg tgccaggccg cgcactaaat cacgggcagc
    11041 aacctgctgg gcgtgctgta gctgttcctg acttaagcgg cgctggttat ccatcggagc
    11101 catctccagc aggatcatgc cgtccggcat acgctgggcc gtcacagaaa ggatatgcga
    11161 gcgcccgtcg atgaccagcg tcacttcgtt atcggtaaaa ccttgccccg cctccagact
    11221 ttcttgcatc agctcgatat ttaatgagaa gtagctcaac agttccggta acggtgtacc
    11281 aaacaatttg cgggagcttt gggcgagcag ttgttgcgcg gcggggttgg cgtaatggat
    11341 cgccagattg tcatcaatta acaaaatact gttaatcagc gagttgagga tctgcccagc
    11401 atcgggctgc gtgcctgttg ccataaagca gtctcctgaa caggttgcac cattttagtg
    11461 cattatagct ttttacggat aaaaagcgcg aagcatcaga gaattgacgg agaaaaaagc
    11521 ccatgcagag atgggctgaa agtttccacg gcaactaaat cccggcgttg ttgcgccggg
    11581 tagtacaact tcaactatta gacgctgtag tacagctcaa actctaccgg atgcggagtc
    11641 atacgcacgc ggtcatcttc ttcgcgacgt agagcgatgt acgcatcaat tgcttcgtca
    11701 gtgaacacgc caccggcttt caggaactcg cggtccagat ccagttcgtt cagtgcttct
    11761 tccagagagc ctgcaacctg tgggatctct ttcgcttctt ctggcggcag gtcatacagg
    11821 tttttgtcca tggcttcgcc cggatggatc ttgttcttga taccatcaag accggccatc
    11881 agcagggcag caaagcacag gtacgggtta gctgccggat ccgggaaacg tacttcgata
    11941 cgacgtgctt tcggagaaga aaccaccgga atacggatag acgcagaacg gttacgcgca
    12001 gagtaagcca gcattaccgg tgcttcatag cccgggacca gacgcttata agagttggtg
    12061 gtcgggtttg ccagggcgtt aatcgcttta gcgtgtttga ttacgccgcc aatgtagtac
    12121 agcgcctgct cagacagacc tgcgtatttg tcgcctgcga acaggttaac gccgttttta
    12181 gacagagaca tgtggcagtg cataccggag ccgttatcac cgaacatcgg ttttggcata
    12241 aaggtcgcgg ttttaccgaa gcggtgcgct acgttgtgca caacatattt gtagatctga
    12301 atttcgtcag cttttttggt catagtattg aagcgggtag ccacttcgtt ctgaccagca
    12361 gtcgctactt cgtggtgatg ggcttcaacc accagaccca tctgttccat caccagacac
    12421 atttcagaac gaatatcctg agccgagtct accggtggaa ccgggaagta accgcctttc
    12481 actgccggac ggtgaccttt gttaccacct tcgtattggg tggaggagtt ccatgcgcct
    12541 tcgatatcgt cgatagcaac gtgggaaccg gagatagatg atccgaaacg gatgtcatcg
    12601 aacaggaaga attcaggttc tggcccgaac agtacggtgt cggcaatgcc agtggaacgc
    12661 aggtaatctt cggcgcgctt cgcaatggag cgcgggtcac ggtcatagcc ttgcagggtg
    12721 ccaggttcaa ggatgtcgca acggataatc agggtggagt cggcgaagaa cgggtcaatc
    12781 actgcggtgg atgcgtctgg catcagcacc atgtcggact cgttaatgcc tttccagccg
    12841 ccaatcgagg agccgtcaaa cattttgcct tcttcgaaga attcagcatt cacctgatga
    12901 gcagggatag tgacgtgctg ttctttacct ttagtatcgg tgaagcgcaa atcaacaaac
    12961 ttcacttcgt gctcgttcag catcgtcagt acgtgttcag cggacatact ttaactctcc
    13021 tggattggtc atggtcgtcg tggtaacgaa atctgcaatt attttggccg tgtcgccgta
    13081 aaaaagataa agcgaaatct gtgccaactt ttaaattgcc cctaaaaggc gttatcatgc
    13141 gcaccatcgt gcaaaagggc tgcaccacga tgtgaatgtt gcaccaatat agtgcttcaa
    13201 tggaaacatt aagcaccatg ttggtgcaat gacctttgga taaccctttt tatgctccgt
    13261 gaaagcgatc acaaagggac tctgcaatac ttgtttgcgg aggatgtttg tgatcctgtt
    13321 ttgtagtgcg attaatccgt gtacaataac gcgctatttc taatgcctga ggcaaagttg
    13381 tgatcgaaaa attgcgtaat atcgccatca tcgcgcacgt agaccatggt aaaaccaccc
    13441 tggtagacaa gctgctccaa caatccggta cgttcgactc tcgtgccgaa acccaagagc
    13501 gcgtgatgga ctccaacgat ttggagaaag agcgtgggat taccatcctc gcgaaaaaca
    13561 ccgctatcaa atggaatgat taccgtatca acatcgttga taccccgggg cacgccgact
    13621 tcggtggtga agttgaacgt gtaatgtcca tggtagactc agtgctgctg gtggttgacg
    13681 catttgacgg cccgatgccg caaacgcgct tcgtaaccaa aaaagcgttt gcttacggcc
    13741 tgaagccgat tgttgttatc aacaaagttg accgccctgg cgcgcgtcct gattgggttg
    13801 tggatcaggt attcgatctg ttcgttaacc tcgacgcgac cgacgagcag ctggacttcc
    13861 cgatcgttta cgcttctgcg ctgaacggta tcgcgggtct ggaccacgaa gatatggcgg
    13921 aagacatgac cccgctgtac caggcgattg ttgaccacgt tcctgcgccg gacgttgacc
    13981 ttgacggtcc gttccagatg cagatttctc agctcgatta caacagctat gttggcgtta
    14041 tcggcattgg ccgcatcaag cgcggtaaag tgaagccgaa ccagcaggtc actatcatcg
    14101 atagcgaagg caaaacccgc aacgcgaaag tcggtaaagt gctgggccac ctcggtctgg
    14161 aacgtatcga aaccgatctg gcggaagctg gcgatatcgt ggcgatcacg ggccttggcg
    14221 aactgaacat ttctgacacc gtttgcgaca cgcaaaacgt tgaagcgctg ccggcactct
    14281 ccgttgatga gccgaccgtt tctatgttct tctgcgttaa cacctcgccg ttctgcggta
    14341 aagaaggtaa gttcgtaacg tctcgtcaga tcctggatcg tctgaacaaa gaactggtac
    14401 acaacgttgc gctgcgcgta gaagaaaccg aagacgccga tgcgttccgc gtttctggtc
    14461 gtggcgaact gcacctgtct gttctgatcg aaaacatgcg tcgtgaaggt ttcgaactgg
    14521 cggtatcccg tccaaaagta atcttccgtg aaatcgacgg tcgtaaacaa gagccgtatg
    14581 aaaacgtgac tctggacgtt gaagaacagc atcagggttc tgtaatgcag gcgctgggcg
    14641 aacgtaaagg cgacctgaaa aacatgaatc cagacggtaa aggccgcgta cgtctcgact
    14701 acgtgatccc aagccgtggt ctgattggct tccgttctga gttcatgacc atgacttccg
    14761 gtactggtct gctgtactcc accttcagcc actacgacga cgtacgtccg ggtgaagtgg
    14821 gtcagcgtca gaacggcgta ctgatctcta acggtcaggg taaagcggtc gcgttcgcac
    14881 tgttcggtct gcaggaccgc ggtaagctgt tcctcggtca cggtgcagaa gtttacgaag
    14941 gtcagattat cggtattcac agccgctcta acgacctgac tgtaaactgc ctgaccggta
    15001 agaaactgac caacatgcgt gcttccggta ctgacgaagc tgttgttctg gttccgccta
    15061 tccgcatgac tctggaacaa gctctggagt tcatcgatga tgacgaactg gtagaagtga
    15121 ctccgacctc tatccgtatt cgtaaacgtc acctgacgga aaacgatcgt cgtcgcgcca
    15181 accgcgcacc gaaagacgat taatttcgtt ttttagttat aaaaaacctg ccatcgct
//
LOCUS       DAEEBB010000100        15084 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEEBB010000100 DAEEBB010000000
VERSION     DAEEBB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.100"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..781
                     /locus_tag="KLF07_002909"
     CDS             <1..781
                     /locus_tag="KLF07_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832876.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="KLF07_002910"
     CDS             complement(984..1337)
                     /locus_tag="KLF07_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBD9832877.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="KLF07_002911"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="KLF07_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBD9832878.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="KLF07_002912"
     CDS             complement(3165..3458)
                     /locus_tag="KLF07_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBD9832879.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="KLF07_002913"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="KLF07_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBD9832880.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="KLF07_002914"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="KLF07_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBD9832881.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="KLF07_002915"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="KLF07_002915"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBD9832882.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="KLF07_002916"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="KLF07_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBD9832883.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="KLF07_002917"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="KLF07_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBD9832884.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="KLF07_002918"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="KLF07_002918"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBD9832885.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="KLF07_002919"
     CDS             10838..11413
                     /locus_tag="KLF07_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9832886.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="KLF07_002920"
     tRNA            11520..11595
                     /locus_tag="KLF07_002920"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="KLF07_002921"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="KLF07_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="KLF07_002922"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="KLF07_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBD9832887.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAEEBB010000101        14965 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEEBB010000101 DAEEBB010000000
VERSION     DAEEBB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.101"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="KLF07_002923"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="KLF07_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBD9832888.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="KLF07_002924"
     CDS             complement(1608..1931)
                     /locus_tag="KLF07_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBD9832889.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="KLF07_002925"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="KLF07_002925"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBD9832890.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="KLF07_002926"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="KLF07_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBD9832891.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="KLF07_002927"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="KLF07_002927"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBD9832892.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="KLF07_002928"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="KLF07_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBD9832893.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="KLF07_002929"
     CDS             7531..8568
                     /locus_tag="KLF07_002929"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD9832894.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="KLF07_002930"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="KLF07_002930"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBD9832895.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="KLF07_002931"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="KLF07_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBD9832896.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="KLF07_002932"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="KLF07_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBD9832897.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="KLF07_002933"
     CDS             10739..11620
                     /locus_tag="KLF07_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9832898.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="KLF07_002934"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="KLF07_002934"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBD9832899.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="KLF07_002935"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="KLF07_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBD9832900.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="KLF07_002936"
     CDS             complement(14017..>14965)
                     /locus_tag="KLF07_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBD9832901.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3784 c         3922 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAEEBB010000102        14735 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEEBB010000102 DAEEBB010000000
VERSION     DAEEBB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.102"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            233..1897
                     /gene="tssM"
                     /locus_tag="KLF07_002937"
                     /pseudo
     CDS             233..1897
                     /gene="tssM"
                     /locus_tag="KLF07_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1908..3260
                     /locus_tag="KLF07_002938"
     CDS             1908..3260
                     /locus_tag="KLF07_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBD9832902.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3284..3766
                     /locus_tag="KLF07_002939"
     CDS             3284..3766
                     /locus_tag="KLF07_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBD9832903.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3810..4745
                     /locus_tag="KLF07_002940"
     CDS             3810..4745
                     /locus_tag="KLF07_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832904.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKKKKKKKKILSPITSFDYIHFITTHPSGIKDTLAWLV
                     NASKLMTEFDDNGKIIFNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSS
                     RAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVA
                     KAVIAGGVATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKL
                     KYAIRDGLKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            4755..5234
                     /locus_tag="KLF07_002941"
     CDS             4755..5234
                     /locus_tag="KLF07_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832905.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5371..6156)
                     /locus_tag="KLF07_002942"
     CDS             complement(5371..6156)
                     /locus_tag="KLF07_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832906.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6486..6562)
                     /locus_tag="KLF07_002943"
     tRNA            complement(6486..6562)
                     /locus_tag="KLF07_002943"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6526..6528),aa:Asp,seq:gtc)
     gene            complement(6695..7426)
                     /gene="dnaQ"
                     /locus_tag="KLF07_002944"
     CDS             complement(6695..7426)
                     /gene="dnaQ"
                     /locus_tag="KLF07_002944"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBD9832907.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7491..7958
                     /gene="rnhA"
                     /locus_tag="KLF07_002945"
     CDS             7491..7958
                     /gene="rnhA"
                     /locus_tag="KLF07_002945"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBD9832908.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7955..8677)
                     /locus_tag="KLF07_002946"
     CDS             complement(7955..8677)
                     /locus_tag="KLF07_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD9832909.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8711..9466
                     /gene="gloB"
                     /locus_tag="KLF07_002947"
     CDS             8711..9466
                     /gene="gloB"
                     /locus_tag="KLF07_002947"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBD9832910.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9538..10896
                     /gene="mltD"
                     /locus_tag="KLF07_002948"
     CDS             9538..10896
                     /gene="mltD"
                     /locus_tag="KLF07_002948"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBD9832911.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10945..11541)
                     /locus_tag="KLF07_002949"
                     /pseudo
     CDS             complement(10945..11541)
                     /locus_tag="KLF07_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11572..12372)
                     /locus_tag="KLF07_002950"
     CDS             complement(11572..12372)
                     /locus_tag="KLF07_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD9832912.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12613..13527
                     /locus_tag="KLF07_002951"
     CDS             12613..13527
                     /locus_tag="KLF07_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9832913.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13524..14327)
                     /gene="dkgB"
                     /locus_tag="KLF07_002952"
     CDS             complement(13524..14327)
                     /gene="dkgB"
                     /locus_tag="KLF07_002952"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBD9832914.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(14490..14566)
                     /locus_tag="KLF07_002953"
     tRNA            complement(14490..14566)
                     /locus_tag="KLF07_002953"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14530..14532),aa:Asp,seq:gtc)
BASE COUNT         4054 a         3480 c         3498 g         3703 t
ORIGIN      
        1 ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt ttaacgggct
       61 ggtcaaagcg atgaacgccg cgccgccgga gagtgaagaa aaactcgcgg tgctgcgcgt
      121 gatgcgaatg ctggaggaca aaagcggacg taacaatcag gtggtgaagc agtatatggc
      181 aaaacgctgg agcgaaaagt tccacggtca gcgcgatatc caggcacaac tgatgtccca
      241 tcttgactac gcgctggctc atactgactg gcacgcagag cgtcaggcgg gcgacggtga
      301 cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga aagaactgag
      361 caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc tgggcgttct
      421 tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc
      481 tgccgatgac aacaaactgg ttgttccaca gtttatcacc cgctacggcc tgcaaagcta
      541 ttttgtaaaa cagcgcgatg aactggttga actgacagcg atggattcct gggtacttaa
      601 cctcacccgc aatgtgaaat acagtgacgc cgaccgtgcg gaaatccagc accagttgac
      661 cgagcagtat atcagcgact acaccgccac ctggcgggct gggatggaca atctgaatat
      721 ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggcga
      781 ccagcctttg cagcgggcgc tgaccgtgct gcgtgacagc acacagccag gcgtcttttc
      841 tgaaaaactc tctgccaaag aacgggagga agccctggca gagccggatt accagttact
      901 cacccgcctc gggcatgaat tcgccccgga aaacagtacc ctggcagtac agaaagacaa
      961 agaaagcacg atgcaggccg tgtatcagca actcaccgag ttgcaccgct acctgctggc
     1021 aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt tacggcttga
     1081 tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc tgcctgctcc
     1141 gctcaaccgc tgggttggca gactgactga ctaggcctgg catgtggtga tggtggaggc
     1201 tgttcattat atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact
     1261 ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg atgccttcga
     1321 acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc tgaagctgtt
     1381 tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat
     1441 tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca aacagaacgg
     1501 tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac gccgcagtgt
     1561 actgaacctt gacagtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct
     1621 ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca tcggcaccag
     1681 cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc gcctgttctg
     1741 ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta gcgtggacgg
     1801 tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca gcggtgggtt
     1861 gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg aatagtaacg
     1921 tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca
     1981 tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac tggaaactgg
     2041 tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac accgccacct
     2101 actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa ggtgcggaac
     2161 tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc ggcccggcgc
     2221 gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt cagcaaatct
     2281 cctttgcgga atccgaccta ccactgatct accgcacaga gcgggcattg cagcttatct
     2341 gcgataagct ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat ctgctctatt
     2401 ttatgcagca cacgcgtaaa cggcttgaac cccagccgaa gagtaacgct gagaacgccg
     2461 cacagaccac ggtcagaacg ctgatttatg ccccggaaac actggcatct tccacaccag
     2521 aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtg cgcagtctga
     2581 cagagaatcc cccacaggcc agtgtgataa agcaaggcag tacggtaaga gggtttatcg
     2641 cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag gtctatccgg
     2701 tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg gtgtggatgg
     2761 cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat acctccccgg
     2821 tacagccgct ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa
     2881 gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc cgcgcacaga
     2941 gtagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct tttgcagatc
     3001 tagtgatgca gcgcgagaaa gagggactaa ccctttccta tatcaaaaat gtcatctggc
     3061 aggcggagcg gggactgggg caggaaaccc ccattgagtc tctgttgacg cagtaccatg
     3121 atgcccgtgc gcagaagcag aatacagatg cgctggaaaa acaaattaat gagcgactcg
     3181 aaggcgtgtt aagccgctgg ctgctgctga agaataacgt catgccagag gcggcaaccg
     3241 gtaccacggc cgaaaaataa ttatttaatt aaaggagatt atcatggcaa atataagtta
     3301 tttatctttg agtggagaga cacaagggct aatttcagca ggttgctcaa cgcttgattc
     3361 tgtcggaaac aaagcgcagc cagaacataa agatcagatc atggtttatg cgttaatgca
     3421 ttccatctca agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga
     3481 taaatcctca ccattgctgg caaaagcact tagtgacaat gaaaaaatgg caatatgtga
     3541 atttatactc taccgaacca gtaaagcggg tatttaccag ccttattata aaataaatct
     3601 gtcaaaagcc cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga
     3661 actggaacca caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt
     3721 agccggtacg aatgcaatga gtaaatggca ggatcgtgta cagtaactta ctgtaataac
     3781 ataataaatt actattgcgg agagataaaa tggctggttt aaggaataac aacaacacac
     3841 agaatgcaca gtgggctgat tatgtgggtg acattcttcg gggggctcag ccgataaacc
     3901 agctagtacc tcagcaccct tatcttaacg acgtcccact cattgatgag ttaaggcatc
     3961 agaacactca tcatgttatt ctcttgacac ttgatgttgc aaaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaat actcagccca ataacatcat ttgattacat tcattttatc actacacatc
     4081 cttcaggtat aaaagataca ctcgcctggt tagttaacgc gagtaaatta atgactgagt
     4141 ttgatgacaa tgggaaaatt atatttaaca ttaatgcctt aaagtataca aaagcaagtt
     4201 actttgaaat acttggagag aaatatataa aaatcacaac atccagcccc tggcttttag
     4261 aaaagctcgg taaatatatt ttctcttcac gagcgcctca agttctggaa ctcgctattg
     4321 gctggcgagg agctttatct gaatcaataa aaggagttaa gttctgtatc tggttttcag
     4381 tagcctggag aaccatagaa tttattatga gttcagagcg agatctggtt aattttcttg
     4441 gcgatttttc aatggatgtg gccaaagcag ttatagccgg tggtgttgca accgctattg
     4501 gaagcctggc ttcttttgcc tgtgttagct ttggctttcc agtaattctt gtcggaggag
     4561 caattttact gacagggata gtgtgtacag ttgttttaaa tgaaatcgat gctcaatgcc
     4621 atttatcaga aaaattaaaa tatgcaatta gagatggact aaaacggcaa caggaacttg
     4681 ataaatggaa aagggaaaac atgactccat ttatgtatgt tcttaacact ccacccgtga
     4741 tatgaggatt aaccatgcgt ttacatgtta aattaaaaga gtttctttcg atgtttttta
     4801 tggccatatt atttttccct gcattcaatg cttctttgtt ttttaccgga gttaaacctc
     4861 tatattcaat aataaaatgc tccacggaaa tattttatga ttggagaatg ttgattctgt
     4921 gctttggttt catgtcgttt tcctttttaa atattcatgt aattttactt acgataataa
     4981 aattattctt aatcaaaaaa acaaaggtag tcaatttcgc cacagacata acaatacagc
     5041 taactcttat atttttacta atagctatcg taatagcacc tttaatagct ccttttgtca
     5101 caggatatgt caatgctaat tatcacccct gcggtaataa tacgggaatt ttcccaggag
     5161 caatctatat taaaaatggc atgaaatgta ataatgaata tatttctcgc aaggaagatt
     5221 cagcagttaa ataattaaac attctaaaac aaacagtttt taactccgga ttccatgtcc
     5281 tttcttgcac tgtctttgtc aagaaaaatt tcttatgcaa agaattttgt attatttccg
     5341 ccagaaattc ctggcggcag atataattta ttattgatag cggttaacca aatcttttac
     5401 catcgcttta taggtctttt gcttaagttg catgatttgc tgctctgtca tatcagtcgt
     5461 actggcttca ttcttgtcgg gcaatggtgg aagcacttta tattcataat tcaccggcga
     5521 ccaggcagcc cactcacctg tagctacatc caccagcgtg aatcgtacca gatagcgcag
     5581 tgacatcgtg tcagtcaatt tttgattttt ataatctgac caaactattg atttcaatcc
     5641 agagtcatat tttcctgtct gcaacgtatc ctggaaaaca ataatcgctt tctgatgccc
     5701 ttttgcagcc acaaaacgca gtgcctgcat ccagttcata ttctcagcac tggcaacatc
     5761 ctcgttctca tttttatctt tgtttttgtt acaagtcaga ggcttttgcc tgtccgggat
     5821 accagagaat gtggaaacag tataatattt tcgcatctcc tcctgcataa tggtttccgg
     5881 tgcgcggttg ccggattgaa caagtattac aggagaatta aggggaacgc ggaactcatc
     5941 tccatcaagc gcagcttgaa tatcctcttc ggatacggcc agagtggttt cattaccgaa
     6001 taaatctttg tctgtcagtt gctggggagt gtttttatta atattacttt gtgtgacttg
     6061 agcttttttc ttcttttcat caatagaaga ttcagagaca catcctgcaa gcagcgagaa
     6121 taatacacat atacaacaaa cccttcttaa attcataatc ggacactccc tcgcctgtca
     6181 taatcatggt aatgtttagc taacgatttt taattatata ctccacttga atcagaacaa
     6241 acaaaaataa cagtatatat aaattcatta aatcaataga aaaatgaaaa tcataacatt
     6301 caaatatatt taaattgaat gatttgatag tatgattaaa tattaatgat atggtctacc
     6361 atatgtgatt aacacattta cagcataaca aaaccataga ccgaagaaat agcgggattt
     6421 aaatgcaaaa aaaccgcaaa attaaaaatc ttgcggttct ctgaactcat tttcatgagt
     6481 gaatttggcg gaacggacgg gactcgaacc cgcgcccccc tgcgtgacag gcaggtattc
     6541 taaccgactg aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga
     6601 ttgcctcacc cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc
     6661 ccaagccgct atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc
     6721 ccttcttctc caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa
     6781 aaacaacgcg taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt
     6841 gctgctgtgt ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca
     6901 gataaacttc cgcaaggatc tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt
     6961 tatctatttc gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt
     7021 tcctcgccac cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc
     7081 gcttaagcaa cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca
     7141 ccaactccgc gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct
     7201 tatcgagcaa aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc
     7261 gatcgggttt gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa
     7321 cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg
     7381 tggtttcggt atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg
     7441 tcagacttat cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac
     7501 aggtagaaat tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg
     7561 ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca
     7621 acaaccgtat ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg
     7681 aagtcatttt gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata
     7741 actggaaaaa acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct
     7801 ggcaacgtct tgatgctgcc ctagggcaac atcaaatcaa atgggaatgg gttaaaggcc
     7861 atgccggaca cccggaaaac gaacgctgtg atgaactggc tcgtgctgcg gcaatgaatc
     7921 ccacactgga agatacgggc taccaagtcg aagtttaagc ttgtggttta cgacattgcc
     7981 gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcgggttta
     8041 gcgttaaagg aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa
     8101 tatgcgcatt caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg
     8161 cgtgcagcac ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca
     8221 tccggctgtt atagggcgag gtttttcgca ataccggcac aagtttgcgt aatcccatta
     8281 aactaatggg attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg
     8341 tttcacgcaa taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa
     8401 catcaacgga tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca
     8461 ttccttgcgc agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg
     8521 cgcttaaatt gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga
     8581 gctggcgctc cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag
     8641 gagccacgac agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactaa
     8701 gaggtaatct atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt
     8761 gaatgatgaa gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa
     8821 cgccattgcc gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga
     8881 tcacgttggc ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc
     8941 acaagagaca caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt
     9001 tttggggcat gaatttagtg taattgccac accgggtcac actttaggac atatctgtta
     9061 cttcagtaaa ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtaggtt
     9121 gtttgaaggg acagcatcac aaatgtatca atcacttaat aagttaagtg cgttacctga
     9181 cgatacattg gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag
     9241 tattcttccg cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc
     9301 aaaaaatcaa ataacactac ccgtaattct gaaaaatgag cggcaaatta atgttttttt
     9361 aagaacggaa gatattgatt taattaatgt aattaatgaa gaaacattat tgcaacaacc
     9421 tgaagagcgt tttgcatggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt
     9481 catcggctcc gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg
     9541 aaggcaaaag cgatattact cgcctctgtc ctgctcgtgg gttgccagag taccggcaac
     9601 gttcaacagc acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca
     9661 agtcaggcac gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct
     9721 ttcattggcg acgagctaaa gatgggaatt ccggaaaatg accggattcg cgaacagaaa
     9781 cagaaatatt tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat
     9841 atgtactgga tagcagggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta
     9901 cccatagtgg agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcaggcatc
     9961 tggcagatca ttccgagcac ggggcgcaat tatggtttga aacagacccg caattatgac
    10021 gcgcgtcgcg atgttgttgc ttcaacaact gccgcgctga atatgatgca gcgtctgaac
    10081 aaaatgtttg acggcgactg gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg
    10141 gtcatgaagg caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta
    10201 ccgttgccgc aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag tgatattctc
    10261 aaaaacagca agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg
    10321 cgtgtgcacc tgagtagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc
    10381 gtcagcaagc tgaagacatt caacgctggc gtgaaaggct ccacgctggg cgcaagtggg
    10441 ccgcagtacg tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctcgcttca
    10501 ggcgaaattg ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt
    10561 gtttacaccg tacgctctgg cgacacgctt tcaagcatcg cttcacgtct cggcgtaagc
    10621 accaaagatt tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagt
    10681 ttgacgattg gtgcaggcag tagcgcacag cggttggcaa acaacagcga tagcattacg
    10741 tatcgtgtgc gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc
    10801 aaagatgtga tgcgctggaa cagcgacact gcgaatctgc aaccaggcga taagctgacg
    10861 ttgtttgtga aaaacaacaa catgccagat tcctgacaaa ccagataata aaaaaggcac
    10921 cgattccccc ggtgcctttt ttatttatgc cgctttatgt gcttctacca tgatgatatc
    10981 actggtgaaa gagccatcag tctgcaagga aaaatacgtt ctcacctctg tcgatgcgct
    11041 ctgttggtaa atgcgaatag cgtctactaa cgcttctggc gtacgcattc tcacgaccca
    11101 tgaagaaaat tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc
    11161 attgattaac gtcaaccacg caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa
    11221 tgcttctacc gcctgtaacc agatgtcgcg cactgggtga cccggagaca ttacatccat
    11281 cacaatcagc ctaccgccag gtttcaatat cctattcact tctcgcagtg ctgcaccaac
    11341 atcatgccag tgatgggcag aataacggct gataacaata tcaaatgcgt tatcggcaaa
    11401 tggcagactt gcggcatatc cctggcgggt ggtgatattt ttcagttgcc gggcttcggc
    11461 agcttgtgcc acgacatcca gcatgtgggc agataagtca tacgccacca ccgcgctcac
    11521 gttttgcgca gcgccaaacg ttggtctgcc ctgcggcaga cctgacatac cttatttatc
    11581 aggcttgccg ggactgaatt caacgagtag cggattgtga tcggaagcgc gcgtaaccag
    11641 tacagaagct tcactgacgt tcagaccacg gtagaaaaca aaatcgagcg ggcgaccaaa
    11701 cgcccgacgg cgctgatcat cggtaaaacg cacctggcgc agcgacattt cccgcgcaaa
    11761 gcgatataac gcgttcatcc ttctacggct ccaggcattg aaatctcccg ccataatgac
    11821 cgggccgctg tggtgagcta tctgatcacc aataggaagt aactgcttac tatagacatc
    11881 cacgcccaga ctgaagttga cggcgtgtat attaaccacc atcaacaggc gggtgtcagg
    11941 taatggatag accgtcacca gtgccgactt cgccagacgc aaaatgggtt ctcgttcacg
    12001 taacgggcag caatacactg gatgtgccgc cgaaagggtc attacgccag aaggatgttg
    12061 tggcagcacg aaagcgggta cctgatcggc ggcaagatag ttagcggtcg caaactgtac
    12121 caactctggc gttgtttgcg cttcctgcaa taacaccaga tgtgcgtctt tgccgtaatt
    12181 ctttaatacc gacaaccatt cagcgcgttg ctgtttgtat atgttccaca ccaggatccg
    12241 aatacgctct tcggtactta acggttctcc gggtggtaat gcctggccga tgctcgcaaa
    12301 agaccccggc ggtaagatcc tctccgcagg ttgtccggca acatagcgca tggcataggt
    12361 gttttttcgc acttttgact ttgaaacctc tattacgtca acccttccgc actcgggagg
    12421 gttctactgt tatagggatt ttagctcact ctttcaacga gcaattgtat attgttatgt
    12481 aagcaagtgc ttacaccggt cagaaatttg ctcattattg agaacattaa atcggatgac
    12541 atatcacgca tcctgatggt ttaatgacaa ataaattcaa aatatctcat ctattttgca
    12601 acaggagcaa aaatgaaagc cacgtcggaa gaactcgcca tttttgtttc ggtcgtcgaa
    12661 agcggcagct ttagccgggc agcggaacaa ttagggcaag caaactcagc ggtaagccgg
    12721 gcggtgaaaa agctggaaat gaaacttggc gttagcctgc ttaatcggac cacgcggcaa
    12781 cttagcctga cggaagaagg cgagcgttat tttcgtcgcg tacagtcaat tttgcaggag
    12841 atggcagcgg cagaatcaga aattatggag acgcgtaata caccgcgtgg actgttacgg
    12901 atcgatgccg caactccagt ggtgctgcac tttctgatgc cgttaattaa gcctttccgt
    12961 gaacgctatc cggaagtcac tttatcgcta gtctcctccg aaacgattat taatttgatc
    13021 gaaagaaaag tggatgtcgc gatacgcgct ggtacgttaa cggactccag cttacgtgcc
    13081 aggccgttat ttaacagtta tcgaaaaatt atcgcctccc ccgattatat ttcccgctac
    13141 gggaagccag aaacgatcga cgatttaaag caacatgttt gcctgggatt cactgaaccc
    13201 gcttccctca atacctggcc gatagcctgt agtgatggac aattacatga ggtgaagtac
    13261 ggtttgtcat ccaatagtgg ggaaacactg aaacagcttt gcctgagtgg caacgggatt
    13321 gcgtgtttgt ccgactacat gatcgacaaa gaaatcgctc gcggagaatt ggtggagtta
    13381 atggcagata aagtgttgcc agtggaaatg ccattcagtg ccgtctatta cagcgaccgt
    13441 gcggtaagta cgcgcatccg ggcttttatc gatttcctta gcgagcatgt aaaaacagct
    13501 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
    13561 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
    13621 ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg aattactgag
    13681 taaccttccc ccatagccca cgccagaatc acttgtgccg gagtcgcatt gtgtttagct
    13741 gcaatacgag caataacctc atctttcagt gccttaccat aagccagcgt catataggaa
    13801 gtgatatgaa taccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
    13861 gagagttcaa tctggttggt agcgatgttt tcagcgccaa cagcagcaat cgctttttcc
    13921 atcaacggga tcgtgaagtt ggaaataccg atctcacgtg tcagaccttg ttttttggcc
    13981 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
    14041 attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt tagactcggg
    14101 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
    14161 cgtggcacgc cactttctgc aatcgcctga cctactgcgg cttcgttatc atagatttgt
    14221 gcagtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
    14281 tcgtctttga gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
    14341 taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgcgg aaaaagagca
    14401 taaaatgcag aggatttttg cgattctggc aataatagat atacagacga aaaaaaacct
    14461 cgagcgttaa ctcgaggctt cttaataagt ggcggaacgg acgggactcg aacccgcgac
    14521 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
    14581 caaccaccgg ttttatgacc ggcttactgc ttaatttgat gcctggcagt ttatgaatca
    14641 cttcgggctg ttgcttcgca acgttcaaaa gctttcgctt ttgtcctact ctcttatcga
    14701 gtagtgaact gcctcaaagg tcagtgccat tcttc
//
LOCUS       DAEEBB010000103        14569 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEEBB010000103 DAEEBB010000000
VERSION     DAEEBB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.103"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="KLF07_002954"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="KLF07_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBD9832915.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="KLF07_002955"
     CDS             2273..2662
                     /locus_tag="KLF07_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD9832916.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="KLF07_002956"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="KLF07_002956"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBD9832917.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="KLF07_002957"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="KLF07_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBD9832918.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="KLF07_002958"
     CDS             complement(4321..4521)
                     /locus_tag="KLF07_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBD9832919.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="KLF07_002959"
     CDS             4687..5232
                     /locus_tag="KLF07_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBD9832920.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="KLF07_002960"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="KLF07_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="KLF07_002961"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="KLF07_002961"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBD9832921.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="KLF07_002962"
     CDS             complement(6529..7353)
                     /locus_tag="KLF07_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBD9832922.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="KLF07_002963"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="KLF07_002963"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBD9832923.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="KLF07_002964"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="KLF07_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBD9832924.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="KLF07_002965"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="KLF07_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBD9832925.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="KLF07_002966"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="KLF07_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBD9832926.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="KLF07_002967"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="KLF07_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBD9832927.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="KLF07_002968"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="KLF07_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBD9832928.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="KLF07_002969"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="KLF07_002969"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBD9832929.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            14120..>14569
                     /locus_tag="KLF07_002970"
     rRNA            14120..>14569
                     /locus_tag="KLF07_002970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3556 a         3663 c         3889 g         3461 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aggtttaatt
    14101 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    14161 gcaggcctaa cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga
    14221 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
    14281 acggtagcta ataccgcata atgtcgcaag actaaagagg gggaccttcg ggcctcttgc
    14341 catcggatgt gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg
    14401 atccctagct ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc
    14461 ctacgggagg cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc
    14521 gcgtgtatga agaaggcctt cgggttgtaa agtactttca gcggggagg
//
LOCUS       DAEEBB010000104        14335 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEEBB010000104 DAEEBB010000000
VERSION     DAEEBB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.104"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="KLF07_002971"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="KLF07_002971"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBD9832930.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="KLF07_002972"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="KLF07_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBD9832931.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="KLF07_002973"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="KLF07_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBD9832932.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="KLF07_002974"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="KLF07_002974"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBD9832933.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="KLF07_002975"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="KLF07_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBD9832934.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="KLF07_002976"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="KLF07_002976"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD9832935.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="KLF07_002977"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="KLF07_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBD9832936.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="KLF07_002978"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="KLF07_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBD9832937.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="KLF07_002979"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="KLF07_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBD9832938.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="KLF07_002980"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="KLF07_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBD9832939.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="KLF07_002981"
     CDS             complement(11402..11899)
                     /locus_tag="KLF07_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBD9832940.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="KLF07_002982"
     CDS             complement(11896..12258)
                     /locus_tag="KLF07_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD9832941.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="KLF07_002983"
     CDS             complement(12248..12595)
                     /locus_tag="KLF07_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBD9832942.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="KLF07_002984"
     CDS             12704..13153
                     /locus_tag="KLF07_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832943.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>14335)
                     /locus_tag="KLF07_002985"
     CDS             complement(13402..>14335)
                     /locus_tag="KLF07_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9832944.1"
                     /translation="FFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMR
                     YRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSD
                     GTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHK
                     VTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQ
                     LAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGAR
                     NILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3387 a         3961 c         3714 g         3273 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggg ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggac ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggca ggctgaccgt acccgctgct gactttgaca tgttgctgac
    13801 cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttgtg
    13861 aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg
    13921 cttctgccag ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag
    13981 cttcttctgg tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt
    14041 ggcgagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
    14101 tgatacttca ctttctgtct gaatactgat gtaccactta ccgcaggact ggctgacagt
    14161 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agcccagttt
    14221 cggcagaaaa atacggctgt tttcctgatc gagcttaacg ccctgcgggt agcggaatac
    14281 atcattctgt ccccgctttt tgaatcgggg aaaagcagcc cgattctgga agaag
//
LOCUS       DAEEBB010000105        14306 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEEBB010000105 DAEEBB010000000
VERSION     DAEEBB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.105"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="KLF07_002986"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="KLF07_002986"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBD9832945.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="KLF07_002987"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="KLF07_002987"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBD9832946.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="KLF07_002988"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="KLF07_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBD9832947.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="KLF07_002989"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="KLF07_002989"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBD9832948.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="KLF07_002990"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="KLF07_002990"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBD9832949.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="KLF07_002991"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="KLF07_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD9832950.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="KLF07_002992"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="KLF07_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBD9832951.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="KLF07_002993"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="KLF07_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBD9832952.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="KLF07_002994"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="KLF07_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBD9832953.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="KLF07_002995"
     CDS             complement(13987..>14306)
                     /locus_tag="KLF07_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBD9832954.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAEEBB010000106        14158 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEEBB010000106 DAEEBB010000000
VERSION     DAEEBB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.106"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..228)
                     /locus_tag="KLF07_002996"
     CDS             complement(<1..228)
                     /locus_tag="KLF07_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBD9832955.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="KLF07_002997"
     CDS             385..561
                     /locus_tag="KLF07_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBD9832956.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="KLF07_002998"
     CDS             1187..1486
                     /locus_tag="KLF07_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832957.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="KLF07_002999"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="KLF07_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBD9832958.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="KLF07_003000"
     CDS             complement(2472..2939)
                     /locus_tag="KLF07_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBD9832959.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="KLF07_003001"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="KLF07_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBD9832960.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="KLF07_003002"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="KLF07_003002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBD9832961.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="KLF07_003003"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="KLF07_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBD9832962.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="KLF07_003004"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="KLF07_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBD9832963.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="KLF07_003005"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="KLF07_003005"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBD9832964.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="KLF07_003006"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="KLF07_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBD9832965.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="KLF07_003007"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="KLF07_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBD9832966.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="KLF07_003008"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="KLF07_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBD9832967.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="KLF07_003009"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="KLF07_003009"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBD9832968.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="KLF07_003010"
     CDS             complement(13837..14082)
                     /locus_tag="KLF07_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBD9832969.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3445 a         3424 c         3690 g         3599 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgt
//
LOCUS       DAEEBB010000107        14107 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEEBB010000107 DAEEBB010000000
VERSION     DAEEBB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.107"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="KLF07_003011"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="KLF07_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBD9832970.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="KLF07_003012"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="KLF07_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBD9832971.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="KLF07_003013"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="KLF07_003013"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBD9832972.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="KLF07_003014"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="KLF07_003014"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBD9832973.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="KLF07_003015"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="KLF07_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBD9832974.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="KLF07_003016"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="KLF07_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBD9832975.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="KLF07_003017"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="KLF07_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="KLF07_003018"
     tRNA            complement(6687..6776)
                     /locus_tag="KLF07_003018"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="KLF07_003019"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="KLF07_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBD9832976.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="KLF07_003020"
     tRNA            7768..7843
                     /locus_tag="KLF07_003020"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="KLF07_003021"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="KLF07_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3353 c         3171 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta atagcagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAEEBB010000108        14012 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEEBB010000108 DAEEBB010000000
VERSION     DAEEBB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.108"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..592)
                     /locus_tag="KLF07_003022"
                     /pseudo
     CDS             complement(1..592)
                     /locus_tag="KLF07_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(749..2929)
                     /locus_tag="KLF07_003023"
     CDS             complement(749..2929)
                     /locus_tag="KLF07_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBD9832977.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(3028..3234)
                     /locus_tag="KLF07_003024"
     CDS             complement(3028..3234)
                     /locus_tag="KLF07_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832978.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            complement(3259..3969)
                     /locus_tag="KLF07_003025"
     CDS             complement(3259..3969)
                     /locus_tag="KLF07_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9832979.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            complement(4445..4750)
                     /locus_tag="KLF07_003026"
     CDS             complement(4445..4750)
                     /locus_tag="KLF07_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832980.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(4897..5112)
                     /locus_tag="KLF07_003027"
     CDS             complement(4897..5112)
                     /locus_tag="KLF07_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832981.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(5333..5623)
                     /locus_tag="KLF07_003028"
     CDS             complement(5333..5623)
                     /locus_tag="KLF07_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832982.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            complement(5947..6243)
                     /locus_tag="KLF07_003029"
     CDS             complement(5947..6243)
                     /locus_tag="KLF07_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832983.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            complement(6243..7196)
                     /locus_tag="KLF07_003030"
     CDS             complement(6243..7196)
                     /locus_tag="KLF07_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBD9832984.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            complement(7579..8241)
                     /locus_tag="KLF07_003031"
     CDS             complement(7579..8241)
                     /locus_tag="KLF07_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD9832985.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            8757..9326
                     /locus_tag="KLF07_003032"
     CDS             8757..9326
                     /locus_tag="KLF07_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9832986.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10054..10227
                     /locus_tag="KLF07_003033"
     CDS             10054..10227
                     /locus_tag="KLF07_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBD9832987.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(10485..11813)
                     /gene="rutG"
                     /locus_tag="KLF07_003034"
     CDS             complement(10485..11813)
                     /gene="rutG"
                     /locus_tag="KLF07_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBD9832988.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(11834..12328)
                     /gene="rutF"
                     /locus_tag="KLF07_003035"
     CDS             complement(11834..12328)
                     /gene="rutF"
                     /locus_tag="KLF07_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBD9832989.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(12339..12929)
                     /locus_tag="KLF07_003036"
     CDS             complement(12339..12929)
                     /locus_tag="KLF07_003036"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBD9832990.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(12939..13739)
                     /gene="rutD"
                     /locus_tag="KLF07_003037"
     CDS             complement(12939..13739)
                     /gene="rutD"
                     /locus_tag="KLF07_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBD9832991.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(13747..>14012)
                     /locus_tag="KLF07_003038"
     CDS             complement(13747..>14012)
                     /locus_tag="KLF07_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBD9832992.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         3844 a         3255 c         2896 g         4017 t
ORIGIN      
        1 ccaagcctta ttacatttaa tctgagcatc gagaatatca tttctgatag ctggagcgta
       61 accggataca tcgctaatat tcattaactc tttgcacgat agcataggcg cgttattgcg
      121 ttcagtggtc acgttacctg cattcttctt actggtcaca ccaccgtcat ttgtggtagc
      181 cgttttgcca gtggctgctg aaatatcagt ttgacctacg attgctttaa agttaatttc
      241 ctgaaaagtt agctggatac gaagaccgtt agagatagta acatctttag aggctttaaa
      301 gttggtaata atggaatcgt caattttaat tccatcttta cagataacag aaataatctg
      361 cttctggtca cgccagtttt ccagagtgtc aatgaagttt tctactaatt gaccctgacg
      421 agttaataat aagcttcctt cataattgca tgttctgtgt agtcacctgc atagggctat
      481 caaattccat gttttcagtt gctgataagg ttaagaaggc atctacattg tctcttaagt
      541 ggaaatagat gccatctttg ccactgtaac gaagctgatt agtgtaaatc attaaattct
      601 cctttgattt caaaggctta gtgtatttta atcattttta accaagcctt tgatatatat
      661 gtattttctc tgttaagcgt gagctacgtg aaaaataatt tttggtgtgg gtattaactt
      721 tgctttattc acgtagaaca ccgttaaatc attcttcctc tggatagctt gaacttaaga
      781 gcatatcctg cgtgaactgg tcattagctg attgattgaa agtaaagaaa gatggttcat
      841 ctggtcgaac gacaacttga ccaccaagtg aaactttcac tggctcctga atacgcttac
      901 tcaaagcact aatttcatcc ctcagaccct gaatatctgc actcgttaat gctgaatctt
      961 gcttaatgtt gtttaactct ggatgattga atcttgcaat attcattgtg gcattgttaa
     1021 acaattcacc cattgaacta aaaattctac tccatccttc gttaatcata ttcggtttat
     1081 tagggtcacc agaatacacg ggattaaagt cactacccac acctacccca gacggatgca
     1141 tattagcgtt tttgactact tcctgattca taaaatccag tgctttcttc aatgcatata
     1201 aaccaagagt gacgcctctg atttgtggaa tcattgaagc taatgctaac gctgtttcat
     1261 aaccgtcatc agcataacca attgcaagag cactacctac accaccagct acacctttca
     1321 gttttccacc acctttacca gtagctacct gaccagccac ctgattagct cttgtaccta
     1381 acgttgtgat tgctcttgtc aggttcataa cccctgtagt agcactgaat agcgtctttg
     1441 cagataacgc tatagcaaga cctttgacga agttgataaa accatcaccg acttcatcaa
     1501 gtagttttcg tttagtgtca tcaaggctgt aatagaactt cttagtctgt aaaatccagc
     1561 cttcaatggt gttattgaat acgcgaatat catctacacc agctttaagc atgtcgataa
     1621 gtccaccgat agcatcacca gcgatagaag aactcatccc catttcacta agcaggttac
     1681 tcaggctgga taagaagccg tttagctcat cctgtgaacc tgagaagatt ttcaccatcg
     1741 tatcatccca attgtttgct aacttaccta aagctgtctg agtgctctta gtgacttcag
     1801 cccagccacc cgctttatca atcatcaagc ccatctgttt agcgacttta gggagagtat
     1861 cagcggaaag taccttcccg tttttcatgg ctgcatcaag ctctgtagca gtcataccca
     1921 atgcttcagc gaatagctta aatgcaccag ccatacggtc acctaactgc ccacgtaatt
     1981 cctcagcctg aatcttgccc ttgctcgcca tctgctcaaa tgctctcaaa atgccgttta
     2041 gctcatcgtt agaaagcccc attaagcgac tcatagccgt tgcattacca aatagctcct
     2101 gtgtctgttc ctgtgacata gaaccctgag cacctgctgc aaacttagcg tagcttgata
     2161 gcccttcaga gatattaaga cctgtcttgt ctgctaaatt tcgaataaat acctcagctt
     2221 cagatgcttt atcaccaagt acaaacttag cggctctctg tgcggaatct cgtttaagac
     2281 ctgcattcat aatggccttg taagcgttaa gagcacccat cacagagtaa aatgcaccag
     2341 ataatttaat cgcattgctt gcaagattat taaatgcgtc gcttgtattg ttaatgctgg
     2401 cggtctttgt ctgtgacctg agtttattca acgtttcacg aagagattga tttaattttg
     2461 caagttcagc gttaaacttt tcacttccaa tattaccagc cttaccgctt aagctgtaat
     2521 actgtgcttt aaacatttta gccatcagcg gattaatatc accaagctta gacatattca
     2581 cgctatgcgc tgctgtgatt gctctgtttc cgctaattcg tggttgcctt gattgttttg
     2641 gaggattaac agtgtcatta aatctggtgg caaactgaaa tgcttttgtg cttcttgctc
     2701 tctgctcaat cttgtcaaag gtcttattaa acttcctttc aagctgccct aaagaacttg
     2761 tcagattgct gtcaagttgc ttggtatcga ccttaaacgc tctattcaaa ttgcgttcaa
     2821 ttctttgtgc tgactgcaaa gctgcctttt caactctttg caagccctta ataacgtttt
     2881 cactgaaact aagttcaacg ataaaactat caactgtgaa tttgctcatt catgtttcac
     2941 ctctttaagt gttcatatac gaaaaaggga aagactaccg atatcactcg ataatctctc
     3001 ccgtagattt tcacccaacc aatcttgtta cctaaccaga taataattac catcttcacc
     3061 aacaatctga caatcacttg gtaatttatc aagacaccac ttagctattt tcctgattct
     3121 attcttttgc ttagaggagg tgaatgattt gattcgtttt ggtttgttgg gttgttcttt
     3181 caatcgttca ctaattgctt taataagctc atagcgttct gttaaatcaa tcattcacca
     3241 ccacctttta attattaatt aaatagcctt tgcatatttg ctagaaatct tcgtaagctg
     3301 ctcaatatca gcctgcatag caatcttctt agcatcttca tattctttca caagctcttt
     3361 catagcctga agctctcttg agagatacaa cttaacatta tcaagttgct tgttacgtga
     3421 tgccaagatg ttctgtctag caatttcagc gagttcttca tcgctcttat tagtcatgta
     3481 aataaactga ccagcacctc ttgatgcctt aaccatcttc acaattcgat gtccagcagg
     3541 aagactacta atcacggctg cgacgttttc ttgaaaagta ccaaaaccat cattatcttc
     3601 gtattggtaa tagacaaatg gtttctgtga caattcatcc tgaatctcaa attctgacgg
     3661 ctcaatagct gattgcatca aggtaacttt gctgtaagcg tcatctaatc taaggcgctg
     3721 ttctttcagc agttggtaat atttcttttc aagctcaagt aatgcttcac tcttgcaaat
     3781 atcaggcatc tcttgaggct caggagacac aaagatggtt gtttttggtg acatctttag
     3841 cgattgctca agctcaatac gcttgtcatg ctctttacgc tgaatagtga tagctggatg
     3901 ttcagtctgt ggcgaccagt cactgaagct atcaatcact ttgccattaa tgtaaatatc
     3961 atttgtcatt gcttttcctc ttctaagcgg aagtgtttca gtttttaaaa agcacacctg
     4021 acagcctatc taaacttaga agagacttaa aaggcggttt gccaagtatg ctttttaaaa
     4081 gctgaatagc cctagataat ttcttataaa gggccattca taaggtgaag aaattaaaac
     4141 atctaaagga ttcaaaaagg attcaaacga atgaacaaga gtacttttcg agatgacctc
     4201 tcagactaac ctgaaaggtc atgtagcgaa aagcattgca cctttcagaa taactatcat
     4261 tgatgtagaa tgattaatag tttaattcag gaagagttgc tcttccctat taccatcatc
     4321 tcatgcttaa ggttatttgt caacagcttt ttaacagatt ggtaacatca attcttattt
     4381 accgtattac ctacccagca accaacaaat aaccctaaag tcatccagag aataacgagt
     4441 gtcattacat cacctcgctg taagggttta attcattctg ataggtcaag aatacattca
     4501 gcttcaataa ttgctcaggg cttaaatcaa gtcgattgca ctcttcaatg ataaagcgct
     4561 tattgtatgg gtctgttact tccagcttgt gaacatagtc accaagttta atttcgttga
     4621 ttatatgctt gtcaatatac gttgacgctt caaaacaatg catgaaatag atattaaagc
     4681 ccgacctgtt caggtcaatc aaccaattca tggtttcttg gtcaaccata atttgcattg
     4741 ggtaattcat ttttaaaatc cttcctgtat cgcattctaa gacgttttaa cccctttttt
     4801 agaccactta aggcgattcc ctaagttacc ctgattaggg gcttaaaaac gtttctggtg
     4861 cgtctggtaa agcctgagag atatgttttg ctgtttttag ctcaagtctg agactaatag
     4921 ccccatctgg tgaccgtagt cttttaacca gctttgctta accggagtct tcagagaata
     4981 gttaggggct gacaagtgaa gatagatatc atctttgccg tggatacttc cccatccttc
     5041 gtataactca agcaagtttt caaaataacg aaatgcttta ttagtctgaa gttgtactgt
     5101 gatagttttc atcaagcacc ccattcagaa ataagcttaa ttccctgctc aagctgttca
     5161 gggtcgttat agtcgagtat taccagtggc tttaattcgt gtttaagctg gtggatttga
     5221 gagggtttta atcgtttgtt tgctttgagg gtcacatctt caaattcagc atctgctaaa
     5281 ttctgtctga ttaggtggcg gccaagtgtg gaatattgga acctataaca cattaaacct
     5341 ccaatctatc cgccagttga tggaccttag cgcttaacac ctgaatcatt gagcaacctg
     5401 ctactgcgga ctgtgctttg agtagttcca gttggcccgt tgtgattctg atggccttag
     5461 aaccctctgc gacgccctta acgttatcga aagggctaag gtttaccgac tcttttaaaa
     5521 tctccccaac aagcgctgtg tgcttcatat cgggatattt ggctttgatt gctgccagtc
     5581 gttcaaatgc ctctggcaaa aggcgcgtaa ctgtgtgagt cattgtaaaa atctccttaa
     5641 atctcttact catactacaa tcttaacatc tatttaacat attgtcagta cctcatttac
     5701 atgatactat ggttgtaggg gctgatacag gtgctgtttc ctttcacaaa aggaaaatta
     5761 atgcatgatt ttttcacagc aattattgaa tctcatcgtt ttagctcgtt gggctttgcg
     5821 gtatttattg tgctattggt gttttgcgtt atgaatcgag tcattacaaa aaagacaaag
     5881 cttacaattc gacttaaact tcttcgtgtt gagatagaga tttcagtaaa taaatagctg
     5941 gtggccttac cggcactttc ccttgataat attggtggcc ctcagattac cggggtcacc
     6001 gtcttttaat cgtagctggt ggactgaatc ccataaatcg taatgataca gcaccgcaaa
     6061 cttcaaagcg tcaatcttta aatctgtcac atcatcgacg aaataataat ccctcccttc
     6121 tttcttcggt cgaatccggt aaacttcccc gtgagtatct ttcatcaagt aaccttcacg
     6181 agaaatgctg taacgtctga gtaaatagtc acggtatttt ctctcgccat tataagtttt
     6241 cattaaaaca ttcctccacc ataaacacgt ctgactttaa ctttttcttt ctttggctcg
     6301 gtgtgagttt tcttttttgg tgtaaaagta aaatcatcgt cactatcatc aaagtcatcg
     6361 aggtcactaa ccttgttctt tagcttcgca tttctgaatc tttctttaat ttctgcttta
     6421 cgctgttcac gtttcaattc ctcttcaggg tcttcttcat catcaaggtc aacatccggt
     6481 aattcatcat cagtggcggg gcactgtgtg tttttatggt cactactagc aatcacatca
     6541 gagattttat cttccttttt aacgacctct ttagcagctt cactatcaag gtcatctaag
     6601 tcagatgttt taacagtctc tttaatagcc tcttcaatac caagagaatc acattcatgt
     6661 gaatcacaga gcgaagctcc cttcttaaaa tattcttctt caaagtcatc ttctttattg
     6721 ttattaataa gttctttctt gttattactg ttattatacg tgtcaaagat tttgactgac
     6781 tgagtaaaaa attttgactc agtgggtaaa gaattttgac tcagtgggtc atctttcttg
     6841 actgactgag taactttaga ctccagcttt gcctttctgg cttccttagc tgtaagctct
     6901 gttaaaacct ccggtacttc atcaagtgaa taaactttaa taacacactg attgcttgca
     6961 acgttagttt tcttgattaa accatgtgcc tcaagttgtt tcatacatgc aatcacggat
     7021 gttcttgtga ttccagcata ctgaccaaga tgagcatatg aaggggtata atattgcccc
     7081 ttatcgtgat aaaacttttg ttcattcaac atgacagtgt aaataatacg gtcggcagct
     7141 ttaacgccat tagtcatctg ataaatacgg tcatcaaaca tgtggaattt ttccattata
     7201 ttttcctttt taaaaataaa acaaagaaag ggctgaccac gtatagtgac caaccccaga
     7261 gggagtgaat taattagaag atggactgat aaaggaactc agaagcctga gcgattacgc
     7321 gagtattaac agcaaaagga aaagccttga ttgaataaga ggccgtgaaa atctgtcaat
     7381 accctgacgc ctcccaacgt tgtaacctct taatgttctg agttctttta tcaccccata
     7441 taaaaactat aacagatgtg attataaatt gcaacaatta tttcacagaa taattactga
     7501 ttggtcattt attgttcaca gggatacttc ttaatatctg aatcacctat cttgtaaatg
     7561 accatattta tgagaatttt agctcaacct ttgagcaatc ctgtatgacg tgctcagagg
     7621 cattctgaga gcatctgcaa tgcacttaat gggttttcct tgcctgtata gttttatgaa
     7681 gctgtcgagc gtctcaggtg ggtaaatggt gggtcttcct ctcaagcctc ctctgttaat
     7741 gatggttaac ggtaatagcg tgtcaccaca gcaaaccgtc tgtgtaattt cgtgcttacg
     7801 tggttctttt ttaaaaacaa cgactgcact aggaaacggt gaacgaatag taagcccgtt
     7861 atggcctgct ggtggaaggt atttaactgc acgatgaaga atacggattt catgacagtg
     7921 gttattgctg taaatgtgcc taagccagta gctggtgttg gctctatgcg gtatcaggaa
     7981 agctactaaa tccgcttcag acgcttttaa gaggaacgac ggaatatcgc tgtacggcag
     8041 gttacagaag accttttcac taacccatga ttgacggctt atatcattgg tgaatcgtgg
     8101 taacagcgtg tcatgctcag ttgcagcacc atctagcgta aaaccaaatt cacggtgtaa
     8161 gttcttaaat aacaacaatg gagttctcca gtaatcagaa cccccatgat gtttcccgat
     8221 taaaatatgc tcaattgaca taagaaataa aaaattttat tgcaatcact cttgacaagg
     8281 tttactctct tttaagataa gagatactta acaggctttc taaacggata tcctttctgg
     8341 aatatctatc tcttatctta aaagtcttta cagatacttt aaagatatct gtattaaatc
     8401 tgtacagtaa cttttcagaa tctgaaaatt tttataaaat cgtagtagcc cctcttcaaa
     8461 ttccccttct aaaaatcttc gaaaatatct gacagatgtc tgtagctgct aagtacccgt
     8521 cacaccagct atgtgacccc ctttgcaaaa aagccaaata tcattgactt tctcggtgaa
     8581 ttgctataaa ggcatctatc taggtcttta actgtataaa tacacatgac aacaaacgcc
     8641 cgttaagtgt ctatataaaa aggtagctgt agctatagac aacaattgtg ataattgatt
     8701 taatggctat agacaagcaa gacagacctt tgttgactat atagagagat aagaacatgc
     8761 gtacattcct ttatgctcgt gtatcagttg atgacctcac cacacagaac caagtagaag
     8821 ctgttaaacg tgctggttat gaagtcagac cttcacgagt aattgaggaa gttattagtg
     8881 gggctacacc agctatggac agaccacaat ttgtaaagct ggtggataag ctggaagaag
     8941 gtgattgtct tgtagtgacc aagattgacc gcttaggaag ggataacatt gacgtacaga
     9001 agaccgtcac aatgttactt ggtatgggtg ttaagctggt ctgtctagat ttgcctgtcc
     9061 aagacctttc aagtgctgaa ggtaaactta ttcttcaact attcagtacc tttgcagagt
     9121 ttgaacttaa ccgcatcaga gagcgaacca gagagggctt agaacgagct aaagcacaag
     9181 gtaaggtgtt gggtcgtcct gtagctaaaa acactacaga ggccgtttta gagcataagg
     9241 ctaagggatt gtcacaaagt caggttgcaa agcttctgaa tctctctgtg agaacggtta
     9301 gccgtcactg gacaaagaca ctgtaaaaaa aacttagtag catcttcata gcctccttca
     9361 tgggggcttt tttgtgtctg taacatatgg tgaccaaaca tctatatatc gttatatagc
     9421 cctgtatata gcactatata tagtgtctgg agagggacga ctaacaccct gcatagttcc
     9481 ctgaaaaggg gcttcacaga ttctctatat atttcaataa gttaggataa aaagggacta
     9541 taccgatact gcatagaatc tgtgtaggat tgcatagtca taggggttgg aacaagccca
     9601 actacacctg ataaagcctg ttattaacag gtagttgaat gtacgtgttt agctatgtaa
     9661 aagcaccaga actttagcca tttctaacca ctcctcgtgt tatctctatt ccgtagcgat
     9721 tcgctaccac ttatttaaag ataagacgtc cttttcagag tgcaaatttc acaaccactt
     9781 atttgattta taacaacttt cacaagcacg taattttgtc gcaaaatgac acatttttat
     9841 ctcatcgcgt ttttttaatc ataagagcgg cttatggata attattggag atgatatcta
     9901 ttctcgctaa gaagctgttg caggatatta ccaaacgcgg gtctacccgc gtcagttcac
     9961 taagcttagt cccacgtagc gaaaatatgg cagccgccat acgccgcgtt aattctatgc
    10021 aatatgatgt ctatacccag acggaggtca gtaatggcaa accatcgagg cggttccggc
    10081 aattttgcag aagaccgcga aagagcatca gaagcaggta aaaaaggtgg acagcacagc
    10141 gggggtaatt tcaagaatga cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag
    10201 agcagtcacg gcaaaagcga caactagccg ggctaatcaa tgacgaatgc atttttgtct
    10261 gtagctcgtc aaaaagccat caccgccggt tacccggtgg ttgatactga tgacaaatgt
    10321 aagcttgcct gatgcgcgat gcttatcagg cctaccagaa gattgcaata tattgaattt
    10381 gcactgtttt gtaggccgga taaggcgttt acgccgcatc cggcatgaac aatgcgtacg
    10441 ttgtcaacaa tctgcaccgc cgggtaaccc cggcggtttt ctgtttatgg ctcctgatga
    10501 acaacttctg gcggtggaac gtcaaccaac cttctactta acaacgcatt gagtaaaatc
    10561 gcgccaaagg tggctgtacc gatccctccc aacgtaaaac cgcccagcgt gagagcaaaa
    10621 tcacccgcgc ccagcactaa ggttactgcg accataatca aattaccgtt ctggcttaaa
    10681 tcgacacggt tttgtaccca gatccttgcg cctgcgacgg caatcagccc gaacacaaca
    10741 attgatgcac caccaatcac cgcggccgga atggtatgga tcagcgcgcc aaatttcggc
    10801 gaaaagccca acagcatggc gatgacggca gcagcaacaa acaccagcgt cgagtagact
    10861 tttgtcacgg ccatcacacc gatattttca gcataggtgg tgacgccgct accgccgaca
    10921 gagccggaaa gcatcgttgc cagaccatca ccgacgaatg ctcgccccat atacgggtcc
    10981 atattgcgtc cggtcatccc ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga
    11041 atcaccgcca cgggcgcaat cagcatcatt gcctgactat taaaagcagg agtggaaaaa
    11101 tgtggcaaac cgaaccaggc agcatggctg acgagagtaa aatcgacggc ttttcccagc
    11161 cctaaaacgt tggtcatcac gccatacagc agacaggcga caattaaacc tacgagaatc
    11221 aataaccgct ggatcatgcc acgggtaaac accgccacca gcccaataca cagcaccgtc
    11281 attaccgcca tccagctatc aaaggctgaa gccgatacac ttttcactgc gataggcgct
    11341 aagttcaggc caatcgccat caccaccgca cccgtcacca ccggcggcat tagtcgttca
    11401 atccagcgtg taccaatttt catcaccacc aggccaatga cggtataaac cagcccacag
    11461 gcgataatcc cgcccagtgc gatgctgata ttcgggttaa tgccctgacc gttaaagccc
    11521 gtcgcggcaa tcaccacgcc gacaaaagcc gcgctggagc cgagataact ggggacgcgc
    11581 ccgccggtaa taaagaaaaa cagtaacgtg ccgatccccg acattaaaat ggaaagattg
    11641 ggatccagcc ccatcagaat cggcattaac accgtcgcgc caaacatcgc caccgcgtgt
    11701 tgaacgccca ttactgccgt ctgagcaaac ggcaatcgtt catccggcgc gaccacgccg
    11761 ctctctgtag aggtcgattt taactgccag tgaggaaaac cgaacattgc catcagctgt
    11821 ctccttaagg aggttaacaa gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca
    11881 ccagcccgta gggtgtggtg tgacgatgaa tcgcttcgat ggcgcaaaac agaatgtcgt
    11941 gggtgccgac gctcaccacc tggctgatgc ggcagtcaaa cgaaaccaga gcctcttcca
    12001 gttgcgggca tccggtcaca cccgtctgcc agcgggcggc ggcaaagcgg tgttccatgg
    12061 gcgttttgcc gccaaaaaga tttgaaagcg gctcctgccc ggcgctcagt gtatttacac
    12121 acagcgttcg attttcattg aataccggcc agacggacgc cccacgattc aggcacacca
    12181 gtaatgtggg cggcgtgtcg gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc
    12241 cggatggacc gtccgtggtg atgatattga ccgccgcgcc catacaggac atcgcatcgc
    12301 gaaaggtttg ttgatcaact atgttcatag tttgctcctt acaacagccc gcaggcttct
    12361 tcaaaggaca gacgtggcag gcgcgcaaaa agtttgctgc tatcgccata gccgatatta
    12421 atcagcagat tgctcttcag cgtgctgccc gcaaaaaagg cgtcgtcaac gtgttgacgg
    12481 tcaaaacccg acatcgggcc ggtatccagt cccagcgcac ggcaagcgac gatcagatag
    12541 gccgcctgca tggagctgtt gcgaaacgct gtttcttcgg caagttgtgg gctggaggta
    12601 aaccaactgc gggcatcacc gtggggaaac agtagtggta accgttcata aaattcactg
    12661 tcccaggcaa cgatagcggt gacgggcgcg gtcagggttt tttgtagatt gccgctggaa
    12721 agcgccgggt gcagacgttc ttttccttct acagtgcgga taaacacgat ccgtgccgga
    12781 gaacagttag ctgatgtcgg cccccatttt atcggggcat aaagctcccg taacgtctca
    12841 tcgctgacgg gtgtctcccg ccagccgttg tgagtgcggg catcggtgaa cagggtgcta
    12901 agcgcacctg ggctaacggc ttcgttcata gcaattcctt acagggcggc ttcacggtga
    12961 tgtaacaggc tggcaagccc gttgagtaac agagcattaa acgtttcggg gtcggtcacg
    13021 ttgcaggcgt gtccgccata gcgcatcacc attttctggc tatcgggcag ggcggcatga
    13081 agctcactgg aacatgctga tggcaccagc agatcatcac tggcgcagat gatttgcacc
    13141 gggcagcgga tgcgatccgc atggtgacta aagtcagcgc gtttgagggc gttaagtcga
    13201 cgcagtaaat tatttttgcc ctgaaaatgc gccagtgcca gcgcgtcttc tgcctcaagg
    13261 cgaggtgcgc gggccgccat ccagtcggcg ggatagagga acaacggctg cgcttccacc
    13321 catgcctgcg cgccgccgct atacagtaat cgttcgcgaa cctgaaaaca gcggcgcgta
    13381 tgggcgttta ttcgtagcca gccgttaacg ctgatcagca cagttaccga cgcgggataa
    13441 tccagcgcca gctgcattcc caccagcgca ccgagcgcat ggccgaccac cgcgtaatgc
    13501 tcaatccctg cggctaccag cgcctgatgc agttccgctg ccatctgggc gatactgtaa
    13561 tcttctgcca gcgtgtcggg attattgccg gtgccgcgct ggtcgtaaca gactacctga
    13621 tactcctgct ccagcaccgc cagttgcggt aaccagtaac tgccgctacc gccaagaccc
    13681 gaaatcaaca ccactacggg cgcatcagca taagggggag gtgagagtga aagtttcatc
    13741 gcggcctcac ttggcgatat gcgcaattgt ggcgatttcc accagcgcgt caggttttac
    13801 cagtccgcac tgaatgcaga atcgcgccgg tttatcaccc ggaaaaaact cggcgtagat
    13861 ttcgttaatc gcggcgtaat ttttccagtc ggtaataaag atgctgttga aggtcacatc
    13921 cgccatcgtg ccacccgccg tctcgatcac cttgcggata gtttccagaa cgtggcgggt
    13981 ttgcgccttt gggtcatcgg caaacagcac gt
//
LOCUS       DAEEBB010000109        13957 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEEBB010000109 DAEEBB010000000
VERSION     DAEEBB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.109"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(29..1300)
                     /locus_tag="KLF07_003039"
     CDS             complement(29..1300)
                     /locus_tag="KLF07_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBD9832993.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(1300..1737)
                     /gene="umuD"
                     /locus_tag="KLF07_003040"
     CDS             complement(1300..1737)
                     /gene="umuD"
                     /locus_tag="KLF07_003040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD9832994.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(1734..1982)
                     /locus_tag="KLF07_003041"
     CDS             complement(1734..1982)
                     /locus_tag="KLF07_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD9832995.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            2377..3303
                     /locus_tag="KLF07_003042"
     CDS             2377..3303
                     /locus_tag="KLF07_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBD9832996.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLF
                     LAGSAGLLQTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRLFGCTLEHWYAEQGCDFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            3300..3611
                     /locus_tag="KLF07_003043"
     CDS             3300..3611
                     /locus_tag="KLF07_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832997.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            3688..4371
                     /locus_tag="KLF07_003044"
     CDS             3688..4371
                     /locus_tag="KLF07_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD9832998.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            4372..4593
                     /locus_tag="KLF07_003045"
     CDS             4372..4593
                     /locus_tag="KLF07_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9832999.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            4607..5041
                     /locus_tag="KLF07_003046"
     CDS             4607..5041
                     /locus_tag="KLF07_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBD9833000.1"
                     /translation="MYGTCETLCRALAAKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            5087..5863
                     /locus_tag="KLF07_003047"
     CDS             5087..5863
                     /locus_tag="KLF07_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833001.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLAMLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRSVSCRVAEDAQRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREGRAEIRSMADALAKIRG"
     gene            6277..6702
                     /locus_tag="KLF07_003048"
     CDS             6277..6702
                     /locus_tag="KLF07_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD9833002.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            6749..7171
                     /locus_tag="KLF07_003049"
     CDS             6749..7171
                     /locus_tag="KLF07_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBD9833003.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSDNGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            7168..7359
                     /locus_tag="KLF07_003050"
     CDS             7168..7359
                     /locus_tag="KLF07_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833004.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            7430..7744
                     /locus_tag="KLF07_003051"
     CDS             7430..7744
                     /locus_tag="KLF07_003051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833005.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDGQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPV
                     AP"
     gene            8128..8655
                     /locus_tag="KLF07_003052"
     CDS             8128..8655
                     /locus_tag="KLF07_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBD9833006.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            8713..8946
                     /locus_tag="KLF07_003053"
     CDS             8713..8946
                     /locus_tag="KLF07_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBD9833007.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            9005..10963
                     /locus_tag="KLF07_003054"
     CDS             9005..10963
                     /locus_tag="KLF07_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD9833008.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTTRQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            11018..11452
                     /gene="psiB"
                     /locus_tag="KLF07_003055"
     CDS             11018..11452
                     /gene="psiB"
                     /locus_tag="KLF07_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBD9833009.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            11449..12168
                     /locus_tag="KLF07_003056"
     CDS             11449..12168
                     /locus_tag="KLF07_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBD9833010.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            12165..12761
                     /locus_tag="KLF07_003057"
     CDS             12165..12761
                     /locus_tag="KLF07_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833011.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            13223..13723
                     /locus_tag="KLF07_003058"
     CDS             13223..13723
                     /locus_tag="KLF07_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBD9833012.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRLARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         2876 a         3816 c         4211 g         3054 t
ORIGIN      
        1 ataacaatat tatgggtgga ttacatattc ataactgggc tactggcaaa tctgtccatc
       61 tggtggtata cgcaggagaa agcatttccc gtttcatttg ccattcaggg gcggttcctc
      121 gccccgcaaa ccacactttc ccttttcccg actggttcag ttcatcgagt gttttcatta
      181 acttttcact gtttttacgg ggctggattt catcaaataa tcccggctgc gctatacccg
      241 atggtgtgaa atcagccagc atgactcctg ccttcatata gcggtaccct tcacgccaga
      301 catggtttaa ggctctgcat gcggcggcaa taatgtcccg gctgtcctgt gtgggcaatg
      361 gaagcttttc cacagcggca ttgctgtaac agggttcttt tactgcaaag ggggatgtcc
      421 gtacaaatgt cgtcacctgc cggcaatact gacgctcccc acgtagtttc tctgcggccc
      481 gctctgcata ctgaacaaca gcctggtgca tggcatcttt gtctgtgatt cgttcaccaa
      541 aactgcgact acagacaatc tgctgttttg ccggtggtgc ttcttccagg gatatgcagg
      601 actcgccgtt gagttcgcgt accgtacgct caagaatgac gctgaagttt ttccggatga
      661 atgccgtgtt agcctgcgcc agctgcagtg ctgtgttaat acccagcgca ttcagctttt
      721 ccgtcagtct gcgtcctact ccccagacct caccaactgg ctgcagcccc agtagcttca
      781 aggtccgatt acggttttct gccgtcagcg cgaccacacc ggaaaactgt ggccattgct
      841 ttgttgccca ctgggcactt ttagccagcg tttttgtagg cgcaatgccc acccccatgg
      901 tgagtcctgt ccagctcttt acctgttccc tgagctgatg accaaaaatc tccggagaga
      961 tgcaatgatt tatcccccgc aaatcaatga acatttcatc aattgagtag ggctcaactg
     1021 cgggagaaag cgactccaga acagccataa cccgttggct catgctgtgg tacagcgcat
     1081 aattgctgga aaatacatgt attttcttct ccaggcgcat ttgtctcacc tgaaaccagg
     1141 gctgccccat tctgatgcca agggcttttg cctccgggct gcgcgcgatc acacagccat
     1201 cgttattgct gagtacgatg accggttcgt tgcgaaggtc cgggcggaaa actttttcac
     1261 atgaggcgta gaaactgttg atatcagcca gtgcaaacat cagcgtaact cccgcgtcct
     1321 gtgtatcacg tgagtgacaa caccaaaaat acagatgttt tccggataca gtgtgcggaa
     1381 ctccgggctg tctgaaaccg gctccagtgc cgggcgtggg cgcaacagca gtcgtttgac
     1441 ggtgaactca ccgtcgatct cagcgataac gatgtccccg tgttgtggtt tttcggccct
     1501 gtccactacc agcagatcac cattctgcac gccagcctgg ttcatcgatt caccgctggc
     1561 gcgcagaaag aaggtggctg caggtctgct gatgcaatag ctgttcagat ccagttcctg
     1621 ctcagcataa tcagtggcag gcgaaggaaa accggcctgg caacgatcgg caaacaacgg
     1681 tcgcacgtaa ctgtcatcgc ctgaagggtc agccggacgg tgataaacgg tactcatgct
     1741 gcaggcagcc agctgtcgtc ctgccagatt tcttccagca gagacaggat acgttcttta
     1801 tccttgtcgt tgcgggctcc tgtgacagat acagacgggg cgctgccctg gtctatacgc
     1861 agccagattt ccgggtagtg tggtttcaga cgttgttcga tttcattctg tagcgcctga
     1921 agtgtgcctg atttgaggtt ttttgtgctc tggcggtcga aaagaatttc aatgcggatc
     1981 atgttctcat ctcccttttc tttatgctgt atgtttatac agtattctaa taactgaatc
     2041 cgcacacagt caaatgtcat tgttgtgact aaaaaacgct caattctctg agtgtgtggc
     2101 tgtatgcctg ctgtgggctg ctgtgtccag cttatccaca gcattttgcg cacggttctg
     2161 tgaataaaat acctggttac ccaggccgtg ccggcacgtt aaccgggctg catccgatgc
     2221 aagtgtgtcg ctgtcgccgg cctcctcacc cggtcacgtt tcgtcgttcc tcctccacgc
     2281 gctgcggctt cggggccgca cctgcattcg tatgcggtcg cccggttaca ggtgcggcac
     2341 ggcctgatgg aggccgcatg tgagaggaga attcccatgc caaactggtg ctcgaatcgt
     2401 atgtattttt ctggtgaacc ggcacagatc gctgagatta aacgactggc cagcggcgca
     2461 gtcacaccgt tttatcgccg cgccacaaat gaaggtattc agctgtttct ggccggaagt
     2521 gccggacttc tgcagaccac tgaagatgtg cagtttgaac cgtgccccgg actgacggct
     2581 gccggacgtg gtgttgtatc gccggagaat atcgcgttca cacgctggct gacacacctg
     2641 cagaacggtg tgctactgga tgaacaaaac tgcctgatgc tgcatgaact ctggctgcag
     2701 agcggtactg gacagcgtcg ctgggaagga ttaccggacg atgtcaggga taccatcacc
     2761 gcacttttca ccgcaaaaag aggtgactgg tgtggcttct ggagtaacga ggatgtatcg
     2821 gtgtggtgga accgtctgtg tgacaacgta ctgccggaaa aaaccatgcc gtttgacctg
     2881 ctgacggttt tgccgacccg cctggatatt gaagtgaatg gctttaacgg cggtgttctg
     2941 aacggtgttc cttctgcata tcactggtat acagaacggt atggcgtgaa gtggccttgt
     3001 gggtatgacc tgaatatcag cagtcagggg gaaaacttca ttcaggtaga tttcgacacg
     3061 ccgtggtgtc agccggaaag cgacgttgtt gcagaattaa gccgcctttt cggctgcacg
     3121 ctggagcact ggtacgccga acagggctgt gatttctgtg gctggcagtt gtatgagcgc
     3181 ggagagcttg ttgatgtgct gtggggggaa ctggaatggt cttccccgac agatgacgat
     3241 gagctgccgg aagtcaccgg gcctgcgtgg atagtcgaca atgtggcgca ttatggcgga
     3301 tgaagtatga cggagacggg cgggcaaccg cccgtttctt ttccggtaaa ggatgtcgcc
     3361 gtgctcctct ttttactgcg ccggctgacg cggcgcggca taaacgctgc ctgtggtcag
     3421 tgtcccgcgt ccggtgggca cgggacgggc gcttttaccg ctcccggctg gcgtcggtga
     3481 cagtgtacgc cagcctgtcg cccttttctg atgaacgccc ttcaagccgc tttcgcggca
     3541 tagccttgcc gtcagaaaga cgacggctgc ggtattccac ggtcggcctg acccgttacc
     3601 ggacgcggtg aacagcccac aggcagcggg gaacgggcac cacaggggtg ccctcccggt
     3661 gtcctctgta agagaaggag cgttgttatg tcccgttttg tcctcggtaa ctgcatcgat
     3721 gttatggccc gtattcctga taacgccatt gatttcatac tgactgaccc gccgtacctc
     3781 gtcggttttc gtgaccgttc cgggcgcacc atcgctggcg ataaaaccga tgaatggctg
     3841 caaccggcct gtaatgaaat gtaccgcgta ctcaaaaaag acgcgctgat ggtcagcttc
     3901 tacggctggc accgtattga ccgctttatg gcggcctgga aacgcgccgg attcagcgtg
     3961 gcagggcacc tcgtgttcac caaaaactac acatccaaat ctgcgtatgt cgcgtaccgc
     4021 cacgaatgcg cttacatcct ggcaaagggc agtccggccc ggccccgtaa ccctctgccg
     4081 gatgtgctgg gctggaaata ttcaggcaat cgtcatcacc cgacggaaaa gcccgttacc
     4141 agcctgcaac cgctgattga gagcttcaca cacccgaacg caattgtgct ggacccgttt
     4201 gcaggcagcg gctcaacctg cgtcgccgcc ctccagtccg gacgccggta tatcggtatc
     4261 gagctgcttg agcagtatca ccgtgccggg cagcaacgcc ttgccgccgt gcaacgggcc
     4321 atgcagcagg gggccgcgaa tgatgactgg tttatgccgg aggctgcgta aatgaactat
     4381 gcaggacacg aaaaactgcg cgccgaagtg gcggaggtgg ccaatgccat gtgcgacctg
     4441 cgtacaacca tgaatgagat ggagcagcgg tacagcttta atgccgacac cctgccggaa
     4501 cgtctggtgc gtcagacgtt gtttcgcgca aaccgcctcc tgatggaggc atataccgaa
     4561 attctggaac tggatgcgtg ctttaaagac tgagaaggag atgagaatgt acggaacatg
     4621 cgaaacgctc tgccgggcgc tggcagcaaa gtatccggga gacatgccgc tgatgctggt
     4681 tatctggtcc ccggaagaga ttcaggccct cgctgacgga atggatattt ccctgtccga
     4741 tcatgaaatc agaaccgtcc tggcgcgcct ggaggacatc ccggaagacc agcggactga
     4801 atccggtatt tcttccggcg tggcgatgga gatcatcaat aacgtgagcg aaaaccgcca
     4861 ggtgaccgtc cctgctgaac tgctggcgtc cctgattcag accgctgaac aggcattgtg
     4921 gaaacgtgaa tgggccgccc gggatcatgg cctcgccgtc ccggaatgcg tcacccgccg
     4981 tcaggcggtg attaatcagg cccgcaccct gctgaaaaac aacagacacg aaaacgactg
     5041 atgttatcgc cgcctccggg cggcgattca gggagaacga ttaatcatga acgaaaacac
     5101 aacactgaac gcactgattt gtcgtcacgc ccgcaacctg ctgctggcgc agggctggcc
     5161 ggaagagacg gatgttgacc agcggaaccc gaagtatccg ggctggatca gcatttatgt
     5221 tctgctggat gcgccccggc tggcgatgtt actcatcaac cgtcacggcg gcgtactgcc
     5281 gccgctcctg gcctccgcca ttcagaaact gaccggaacc ggggcggaac tggtactgtc
     5341 tggcagtcag tggcagtcgc tgccggtact tccggcagac ggaacacagg tgtttttccc
     5401 gtatgccgga gaatggctga ccgaagacga aatcagggca gttcttgatg cagtgcgcga
     5461 tgcggtacgc agtgtcagtt gccgggtggc agaagatgcg cagcgtatca gggcggcgct
     5521 gaccacaacc ggtcagacgt tgctgacccg ccagacccgc cgctttcgcc tggtggtgaa
     5581 ggaaagcgat cacccctgct ggctcgatga ggacgacgaa aacctgcccg tggtgctcga
     5641 tgccatcctg aaccggggcg cacgtttttc ggcggtggaa atgtatctgg tcagcgattg
     5701 tattgagcat atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac cggatgaacc
     5761 gccccgccgg tggtttgacc gtggtgttct gcgggaggtg gtccgggaag gccgggctga
     5821 aatccgcagc atggcggatg ccctcgcaaa aatccggggc tgatgacgat ggcggggaaa
     5881 tccccgcctt tttcctgccg gtcccggaca gagcacaaag gacgtcgccg ccggctccgc
     5941 tttacccggc catgcgggca tggccttatg ggttttcagt tatgtggcct cagcgccgtg
     6001 tgcgggctgt gccgtgcctc catcttagcc gggctggcgt tgatgcaagg gtacgcttcg
     6061 ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc gttcgcccgc
     6121 tcaggtttcg cggcctcacc ctgcaactcc cgccagccgt gtgcgtcagg ctgcggcttc
     6181 ccttgcgccc ctgcatcccc gccttatcgc ccggctttta tggaggcacg gcaccgcccg
     6241 gtgtcgcgga atatgtgaac aacggaggaa aacatcatgc aatatgcgaa acctgtcact
     6301 ctgaacgttg aagagtgcga ccgtctctcc tttctgcctt acctgtttgg caacgatttt
     6361 ctgtatgccg aggcgtatgt gtacgcgctg gcgcaaaaaa tgatgccgga atatcagggc
     6421 ggcttctggc acttcatccg cctgccggac ggtggcggct acatgatgcc ggacggtgac
     6481 cgtttccaca tggtgaacgg tgcaaactgg tttgaccgta ccgtgagtgc tgatgccgca
     6541 ggcatcatcc tgacctccct tgtgattaac cgccagttgt ggctgtacca cgacagcggg
     6601 gatgcaggac tgacccagct ttaccggatg cgcgatgcgc agttgtggcg tcacatcgaa
     6661 tttcaccctg aatgcaacgc gatttacgcg gcgctggact gattaacgga cggggcggca
     6721 atgccgcccc tgtaaaggag acgagaacat gtactgtact gttaaagaaa ttatccgcga
     6781 agtgctggat acagacgtgc cggacagtga atgcgttttt gccgtggtgc tgacccgtgg
     6841 ggatgtgcgt cacatagccc aggactggag tctgacagac gatgagctgg aaaccgtcat
     6901 gcagcgactg gacgatgcct ttgagcatgg tgcggatgtc agcgttgttc acgacgttgt
     6961 tcgtgagctg atggaagaaa agcgcgccag ccgtcaggtg acagttccgg cggtgatgct
     7021 ggaaaaagtg atggcgctgg caggcagtga aatgaagcgc ctgtatgccg tcgggagtga
     7081 taacgggggc gacggtgacg cattcgtcag ggaggaacgc gaagcaatgg acgttgtgtt
     7141 acaggcgctg gacggggaga cgatgtcatg aatatcagca cagaaacccg cgaaattctg
     7201 cgcaattaca aagccgtgat taatgcgcgg cgtcgtgaaa tggggcagaa accgctcacc
     7261 actgcgcaga ttgttgatga aatctgcgat tttgtggcga atcagcaggc ggttttcctg
     7321 ggcggtcact acatccttca gggcagcaga aacaggtgat acagggcagg cggtggaggc
     7381 cgcctgtcag cccgcgcgaa aacccctggc aggctggcgc ctgccggaaa tgcggggtgc
     7441 agcgcctgcg gcgctgccgg cccggctttc cggtcagaag gtagtgtatg gctgtatgca
     7501 gggaacaggt gtgatattcc gtgacactgt caccgtttct ggctgtcttt tattgcgccc
     7561 acttcggctc ccggctgcct ggcggcatcc ggtcgtcagg cccgactccg acgggcagct
     7621 gctggcgcac ccgccagtct ccgccgaacc cgctggcgcg gtttcgggct gccgctacgg
     7681 tccgatgcgt gaaaacccgg cttgcagcga ccgttccggt cgcgccgccc ctgtcgcgcc
     7741 gtgacctgcg ctccctgcac acctgacggt gtgtgaggaa actgcgcctg atggcgcagc
     7801 agtcctccct cctgccgcca cccctgcggc ctgtttcccg ctgtggactt tccggggggc
     7861 agacgtaacc ccctcccgtt ccatctgtcc agggtgagat gcaccggcca caaaattttt
     7921 agccatgaac ccggctaaaa attttgcgtc cgcccccgga cagctgtccc gtgaggcggt
     7981 aaacgggccc ccatcggaaa ggtccactgc ggggagaaac ccctgtgaag tggacggcag
     8041 gaggagcttt cgctcctctg aaggggcaca tgaacgtccc gtcgccggta tccggtttat
     8101 ctcacatctt tcagtcagga ggttcttatg agtgcacgtg gtatcaacaa ggtcatcctc
     8161 gtcgggcgtc tgggcaatga tccggaagtc cgttacatcc ccaacggggg cgcagtggca
     8221 aacctgcagg tggccacgtc agaaagctgg cgcgacaaac agacggggga gatgcgggag
     8281 cagacggaat ggcaccgcgt ggtgctgttc ggcaagctcg cggaagtggc tggtgaatat
     8341 ctgcgcaagg gcgcgcaggt ctacatcgag gggcaactcc gtacccgtag ctgggaagat
     8401 aacggtatca cccgttacgt cactgaaatt cttgttaaga ccacgggcac cgtgcagatg
     8461 ctgggacgtg caccacagca gaacgctcag gcgcaaccga agcctcagca gaatgggcag
     8521 ccacagagtg ctgacgcgac gaaaaaaggt ggcgcgaaaa cgaaaggccg tggacgtaag
     8581 gccgcgcagc cagagcctca gccgcaaccg ccggaggggg aggattacgg gttttcagac
     8641 gacatcccgt tctgatcggg ctgactgtga caaccgcccc gccctgtgcg gggcatcacc
     8701 ggagatatga ggatgagcga atatttcaga atacttcagg gactgccgga cggctccttt
     8761 acccgcgaac aggcggaagc cgttgccgca cagtaccgga acgtctttat cgaggatgat
     8821 cagggaacgc attttcgcct ggttgtccgt caggatggca cgttgatctg gcgctcctgg
     8881 aattttgagg actgtgccgg gtactggatg aaccagtaca tcagggattt cgggattctt
     8941 aagtaagaga ggtgccggac gcgcgaacgt ccggcaggac ataagcaatt ataaggggat
     9001 gattatgcct gtaacgaagt gtgaaccaga aaccacccgc aaagcaagcc gtaaatctgt
     9061 aaaaacgcag gaaactgtcc tgtctgccct gctggcgcag acggaggaag tgagcgtgcc
     9121 gctggcctcg ctgattaaat caccgctgaa tgtgcgcacg gtgccgtatt ctgcggagtc
     9181 cgtcagcgaa ctggcggagt ccattaaggg cgtcggactg ctgcagaatc tggtcgtgca
     9241 taccctgcct ggtgaccgtt acggtgtcgc cgcaggtggt cgccgactgg ctgcactcaa
     9301 catgctggca gagcgtggca tcattccggc tgactggcct gtacgcgtga aggttattcc
     9361 gcaggagctg gcgacggctg catcaatgac tgaaaacggt catcgtcggg atatgcaccc
     9421 tgccgaacag attgccggat tccgtgcgat ggcgcaggaa ggcaaaacgc ctgcacaaat
     9481 cggtgatttg ctgggctatt cgccccgcca cgttcagcga atgctgaaac tggcagacct
     9541 tgcgcctgtc atcctcgatg cgctggcaga agaccgcatc accacagagc actgtcaggc
     9601 gctggcgctg gagaacgaca ccacgcgtca ggtgcaggtg tttgaagccg cctgccagtc
     9661 gggatggggc ggtaaaccgg aagtacagac cattcgtcgt ctggtgaccg aaagtgaagt
     9721 ggcggtggca ggtaacagta aattccgctt cgtgggggct gatgctttct cacctgacga
     9781 actgcgtact gatttgttca gcgatgacgg ggacggttat gtcgaccgcg tggcgctcga
     9841 tgctgccctg ctggaaaaac tccaggctgt cgctgaacac cttcgggaag ccgaaggctg
     9901 ggaatggtgc gccggacgca tggagcctgt cggtgagtgc cgtgaggatg ccggaacata
     9961 ccgctgtctg ccggagccgg aagcggtgct gacggaggca gaagaagaac gcctgaacga
    10021 actgatgacg cgttacgacg cgctggaaaa ccagtgtgag gaatccgatc tgctggaagc
    10081 agaaatgaag ctgattgact gcatggcgaa ggtcagggcg tggacgccgg agatgcgtgc
    10141 cggaagtggc gtggtggtgt cctggcgtta tggaaacgta tgtgtccagc gtggtgtgca
    10201 gttgcgcagt gaagatgacg cgactgatga cgctgaccgc acggaacagg tgcaggagaa
    10261 agcgtcagtg gaggaaatca gtctgccgtt gctgacgaaa atgtcttccg aacgcacgct
    10321 ggcagtccag gcagcactga tgcagcaacc ggacaaatct ctggcactgc tggcatggac
    10381 gctctgcctg aatgtgtttg acagcggagc gtacagtaaa ccagcacaaa tcagcctgga
    10441 atgtaaacat tattcgctga ccagcgatgc gccgtcaggg aaggaaggtg ccgcattcct
    10501 ggcgctgatg gcagaaaaag cccgtcttgc ggccctgtta ccggagggat ggtcacggga
    10561 catgacgacg ttcctgtcac tcagtcagga ggtgctgtta tccctgctca gtttctgcac
    10621 cgcatgcagc cttaacggtg tccagacccg cgagtgtggt cgcacgtcac gcagtccgct
    10681 tgactcactg gagagcgcca tcggttttca catgcgcgac tggtggcagc cgacgaaagc
    10741 aaacttcttc ggacacctga aaaagccgca gattatcgca gccctgaatg atgccggact
    10801 gtccggtgcc gcacgggacg cagagaagat gaagaaaggc gatgccgctg aacatgcaga
    10861 gttccatatg aaagacaacc gctgggtgcc tggctggatg tgttcaccac gtccacagac
    10921 ggatgccact gaacgcgccg ataacctggc tgatgccgcc tgatgaacaa ccacaccgcc
    10981 ccgccggaga cggggcggca gagggagaaa tacgatgatg aataccgaac tgacactgaa
    11041 tgccctgcag tccatgaatg cccaggaata tgaagaaatc cgtgctgcgg gaagcgatat
    11101 gcgccgtaat ctcactcacg aggtgatgcg taaagtggac gcaccggcta actggatgat
    11161 gaacggagag tatggcagcg agttcggggg ctttttcccc gtccaggttc gtttcacgcc
    11221 agcccacgaa cgtttccacc tggcattatg ttcgccggga gacgtctctc agctctggat
    11281 gctggttctg gtgaatggtg gtggacagcc tttcgccgtc gttcaggtgc aacatatctt
    11341 cacgcctgcc gcgatcagtc acacgctggc gcttgccgcg acactggatg cgcagggata
    11401 cagtgttaac gacatcatcc atatcctgat ggcagaagga ggtcaggcat gagcgcacgt
    11461 tcacgggcac tgatccccct cagcgcagaa caacaggccg ccatgcaggc ggtggctgtc
    11521 acagaacaac gtcgtcgtca gggacgcaca ctttcagcat ggccttatgc caccgctttc
    11581 tttcgctgcc tgaatggcag tcgccggatt tcgctgaccg atctccgctt ttttgcccct
    11641 gcgctgacga aggaggaatt tcatggcaac cgtctcctgt ggctggctgc cgtggataaa
    11701 ctgattgaaa gttttgggga agtctgtgtt cttcccctgc catccgatgc ggggcaccgt
    11761 ctgttcccgt ccgtgccttt tcgtgaaggt gagcgtcgtc gtcagaaaac cacgctgaca
    11821 gagcagaaat acagccgcca gcgggaacgt gaggcagaac gacgggaact ggaataccag
    11881 acatgttttg ctcaggcgca gattgacctt gcgtttcata ctcccgccac ggtcggaagc
    11941 tggttgtccc gctggtctgg tgttgttgag gagcatgatc tggaaacgat tttctggggg
    12001 tggtgcgggc gttttccatc actgtcatca tttgaccggt ttttctggca ggaggaacca
    12061 ctctggcggc tgatttttga agccggtgag gccggtcgtg gtgcaccggt acaggtacgt
    12121 gcacttgagc agtggatgat cccgaacaag ctggagaacg taatatgatg aaatcagacg
    12181 aaaaatacca ggttcccgcg tggatgcgac ctctgttgcc gttgctctgc aacaccgggg
    12241 ggaacgatcc ggaagaactg ctgaatgata cagaaaccac tgccagtgcg aatgttgtcc
    12301 gttatgtact gatagttgct gtgcggtcgc aggttgatct gctgcagctt ctgtacagga
    12361 aagggctgtt gcgcacagag ataccaggtg gcttttcacc ggaagaagcg caggcactcc
    12421 tggataatct ggtgcgcagc catatcagca aggcgctgtc gggcgagcga atggcagccc
    12481 gtgacagaaa tgccgatctg gcctggattg gacagcaact ggtcgatgcc gcctggtttg
    12541 tccgtgccac actggaagcg catgggatga gcgtcggaaa tgagagtccc cctgctccgc
    12601 cggagacaat gccggacata cagacacggg aactggtgat gttgatcaag cgactggcat
    12661 catcgctgaa agcggtgaaa ccggacagtt gtgtggtgcg tgaagcgcag gactggctgc
    12721 gcgacagaaa acttgtggat atcacagata ttctccggtg aaccaggcat gtatatttta
    12781 cgcagggaca cgccgatcat gatcgtttca tatcaggggt ggtctggagg tcatgcggcg
    12841 tgtcctctgc actcgccgga ataaggaagt cgccggcggc tccgctttta cccggccatg
    12901 cggggcatgg ccttgtgggt tttcagctct gtggcctcag cgtcgtgtgc gggctgtgcc
    12961 gtgcctccat cttagccggg ctggcgttga tgcaagggta cgcttcgccg ctgcggtcac
    13021 ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cgtccgctca ggtttcgcgg
    13081 cctcaccctg caactcccgt cagccgtgtg cgtcaggctg cggcttccct tgcgcccctg
    13141 catccccgcc ttatcgcccg gcttttatgg aggcacggca ccgcccggtg tcgcagaaca
    13201 tgtgactatg gaggattcgg gaatgtctgt tgttgcacct gctgtatacg ttggaacctg
    13261 gcacaaatac aactgtggaa gcatcgccgg acgctggttt gacctgacca cgtttgatga
    13321 tgagcgcgac tttttcgccg cctgccgtgc tcttcaccag gatgaagccg atcctgaact
    13381 gatgtttcag gattatgagg gattcccggg gaatatggcc tctgaatgcc atatcaactg
    13441 ggcctgggtt gaaggcttcc gcctggcacg ggatgaaggc tgcgaagagg cttatcgtct
    13501 ctgggtggag gataccggtg agacggattt tgacaccttc cgcgatgcct ggtggggcga
    13561 ggctgacagt gaggaggctt ttgcggttga gttcgccagt gataccggcc tgctggctga
    13621 cgtgccggag acggtggcgc tctattttga ctatgaggcg tatgcgcggg atttattcct
    13681 ggactccttc acctttattg acggtcatgt gttccgtcgg tgatttactc ccccgctccg
    13741 gcgggggtat ggcctgccag gccgccggaa aaccctggca ggcttacgcc tgcctgaaat
    13801 gcaggagggc cgcgcctgcg gcgctgccct tcctgcccgt catggtgtga ggcatttttc
    13861 tgttatgtcg tttctgttgt tctgatttcc ggaggcgggg acaccttcgg ctcccggctg
    13921 cctggcggca tccggtcgtc aggcccgact ccgacag
//
LOCUS       DAEEBB010000110        13903 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEEBB010000110 DAEEBB010000000
VERSION     DAEEBB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.110"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="KLF07_003059"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="KLF07_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBD9833013.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="KLF07_003060"
     CDS             complement(1550..1723)
                     /locus_tag="KLF07_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBD9833014.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="KLF07_003061"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="KLF07_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBD9833015.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="KLF07_003062"
     CDS             3361..3690
                     /locus_tag="KLF07_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBD9833016.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="KLF07_003063"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="KLF07_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBD9833017.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="KLF07_003064"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="KLF07_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBD9833018.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="KLF07_003065"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="KLF07_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBD9833019.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="KLF07_003066"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="KLF07_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBD9833020.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="KLF07_003067"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="KLF07_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBD9833021.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="KLF07_003068"
     CDS             9613..9909
                     /locus_tag="KLF07_003068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833022.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="KLF07_003069"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="KLF07_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBD9833023.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="KLF07_003070"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="KLF07_003070"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBD9833024.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3230 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAEEBB010000111        13855 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEEBB010000111 DAEEBB010000000
VERSION     DAEEBB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.111"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(487..750)
                     /gene="rpsT"
                     /locus_tag="KLF07_003071"
     CDS             complement(487..750)
                     /gene="rpsT"
                     /locus_tag="KLF07_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBD9833025.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            853..1071
                     /locus_tag="KLF07_003072"
     CDS             853..1071
                     /locus_tag="KLF07_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBD9833026.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            1079..2020
                     /gene="ribF"
                     /locus_tag="KLF07_003073"
     CDS             1079..2020
                     /gene="ribF"
                     /locus_tag="KLF07_003073"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBD9833027.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            2063..4879
                     /gene="ileS"
                     /locus_tag="KLF07_003074"
     CDS             2063..4879
                     /gene="ileS"
                     /locus_tag="KLF07_003074"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBD9833028.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4879..5373
                     /gene="lspA"
                     /locus_tag="KLF07_003075"
     CDS             4879..5373
                     /gene="lspA"
                     /locus_tag="KLF07_003075"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBD9833029.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5498..5947
                     /gene="fkpB"
                     /locus_tag="KLF07_003076"
     CDS             5498..5947
                     /gene="fkpB"
                     /locus_tag="KLF07_003076"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD9833030.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5949..6899
                     /gene="ispH"
                     /locus_tag="KLF07_003077"
                     /gene_synonym="lytB"
     CDS             5949..6899
                     /gene="ispH"
                     /locus_tag="KLF07_003077"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBD9833031.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6965..7879
                     /gene="rihC"
                     /locus_tag="KLF07_003078"
     CDS             6965..7879
                     /gene="rihC"
                     /locus_tag="KLF07_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBD9833032.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            8046..8867
                     /gene="dapB"
                     /locus_tag="KLF07_003079"
     CDS             8046..8867
                     /gene="dapB"
                     /locus_tag="KLF07_003079"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBD9833033.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            9323..10471
                     /gene="carA"
                     /locus_tag="KLF07_003080"
     CDS             9323..10471
                     /gene="carA"
                     /locus_tag="KLF07_003080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBD9833034.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            10489..13710
                     /gene="carB"
                     /locus_tag="KLF07_003081"
     CDS             10489..13710
                     /gene="carB"
                     /locus_tag="KLF07_003081"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBD9833035.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3260 a         3436 c         3866 g         3293 t
ORIGIN      
        1 attatcagtc cttgaagttg atgaattttt attaatttaa tttatgagtg aatgtgacta
       61 ctatcaaaaa tagctcatta attttatgaa gtagaatata aataggtatt taacctgtgt
      121 tgattgttgt taattattta caagatctaa taaggagata tttatgaatg gttaaagtgt
      181 ataggaattt aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa
      241 tatccacatt ctttgtaggg gagtctggag attgagtaga tattcttgtt cagaatgtat
      301 cagccgatgg ttctacgatt cttaagccac gaagagttca gaaagtgtaa cggcatgtct
      361 gcaaaagcca gcatcttttg actatctggc aaccggtagt gtgttctctc acgcatcata
      421 aaagcagcag gcataaaaaa aacccgcttg cgcgggcttt ttcacaaagc ttcagcaaat
      481 tggcgattaa gccagtttgt tgatctgtgc agtcaggtta gccttatgac gtgcagcttt
      541 gtttttgtgg atcagacctt tagcagcctg acggtccacg atcggttgca tttcgttaaa
      601 tgctttctgt gcagcagctt tgtcgccagc ttcgatagct gcgtatactt tcttgatgaa
      661 agtacgcatc atagagcgac ggcttgcgtt gtgcttacga gccttttcag actgaatggc
      721 gcgcttctta gctgatttga tattagccaa ggtccaactc ccaaatgtgt tctatatgga
      781 caattcaaag gccgaggaat atgccctttt agccttcttt tgtcaatgga tttgtgcaaa
      841 taagcgccgt taatgtgccg gcactcgtta cgtagtgatg gcgcaggatt ctaccagctt
      901 gcggggtgtg aatacagctt ttccgcgata aaaattgcag caggcggtca ctttcttccc
      961 gtgatttgcg ccatggcaat gaaaagccac ttctttctga tttcggtact caatcgccgg
     1021 ttaaccttga ccgctgtaca aggtatactc ggacgatttt cactgttttg agccagacat
     1081 gaagctgata cgcggcatac ataatctcag ccaggccccg caagaagggt gtgtgctgac
     1141 tattggtaat ttcgacggcg tgcatcgcgg tcatcgcgcg ctgttacagg gcttgcagaa
     1201 agaagggcgc aagcgcaact taccggtgat ggtgatgctt tttgaacctc aaccactgga
     1261 actgtttgct accgataaag ccccggcaag actgacccgg ctgcgggaaa aactgcgtta
     1321 ccttgcagag tgtggcgttg attacgtgct gtgcgtgcgt ttcgacaggc gtttcgcggc
     1381 gttaaccgcg caaaatttca tcagcgatct tctggtgaag catttgcgcg taaaatttct
     1441 tgccgtaggg gatgatttcc gctttggcgc tggtcgtgaa ggcgatttct tgttattaca
     1501 gaaagctagc atggaatacg gcttcgatat cactagtacg caaacttttt gcgaaggtgg
     1561 cgtgcgtatt agcagcaccg ctgtgcgtca ggcgctggcg gatgacaatc tggctctggc
     1621 agaaagttta ctggggcacc cgtttgccat ctccgggcgt gtagtccacg gtgatgaatt
     1681 agggcgcact ataggtttcc cgacggcgaa tgtaccgcta cgccgtcagg tttccccggt
     1741 gaaaggggtt tatgcggtag aagtgctggg cctcggtgaa aagccgttac ccggcgtggc
     1801 aaacatcgga acacgcccaa cggttgccgg tattcgccag cagctggaag tgcatttgtt
     1861 agatgttgca atggaccttt acggtcgcca tatacaagta gtgctgcgta aaaaaatacg
     1921 caatgagcag cgatttgcgt cgctggacga actgaaagcg cagattgcgc gtgatgaatt
     1981 aaccgcccgc gaattttttg ggctaacaaa accggcttaa gcctgttatg taatcaaacc
     2041 gaaacacgga accgagaatc tgatgagtga ctataaatca accctgaatt tgccggaaac
     2101 agggttcccg atgcgtggcg atctcgccaa gcgcgaaccg ggaatgctgg cgcgttggac
     2161 tgatgatgat ctgtacggca tcatccgtgc ggctaaaaaa ggcaaaaaaa ccttcattct
     2221 gcatgatggc cctccttatg cgaatggcag cattcatatt ggtcactcgg ttaacaagat
     2281 tctgaaagac attatcgtga agtccaaagg gctttccggt tatgactcgc cgtatgtgcc
     2341 tggctgggac tgccacggtc tgccgatcga gctgaaagta gagcaagaat acggtaagcc
     2401 gggtgagaaa ttcaccgccg ccgagttccg cgccaagtgc cgcgaatacg cggcgaccca
     2461 ggttgacggt caacgcaaag actttatccg tctgggcgtg ctgggcgact ggtcgcaccc
     2521 gtacctgacc atggacttca aaactgaagc caacatcatc cgcgcgctgg gcaaaatcat
     2581 cggcaacggt cacctgcaca aaggcgcgaa gccagttcac tggtgcgttg actgccgttc
     2641 tgcgctggcg gaagcggaag ttgagtatta cgacaaaact tctccgtcca tcgacgttgc
     2701 ttttcaggcg gtcgatcagg atgcactgaa agcaaaattt gccgtaagca acgttaacgg
     2761 cccaatctcg ctggtgatct ggaccactac gccgtggact ctgcctgcga accgcgcaat
     2821 ctctattgca cctgatttcg actatgcgct ggtgcagatc gacggtcagg ccgtgattct
     2881 ggcgaaagat ctggttgaaa gcgtaatgca gcgtatcggc gtgaccgatt acaccattct
     2941 cggcacggta aaaggtgcgg agcttgagtt gctgcgcttt acccatccgt ttatgggctt
     3001 cgacgttccg gcaatcctcg gcgatcacgt taccctagat gccggtaccg gtgccgttca
     3061 caccgcgcct ggccacggcc cggacgacta tgtgatcggt cagaaatacg gcctggaaac
     3121 cgctaacccg gttggcccgg acggcactta tctgccgggc acttatccga cgctggatgg
     3181 cgtgaacgtc ttcaaagcga acgacatcgt cgttgcgctg ctgcaggaaa aaggcgcgct
     3241 gctgcacgtt gagaaaatgc agcacagcta tccgtgctgc tggcgtcaca aaacgccgat
     3301 catcttccgc gcgacgccgc agtggttcgt cagcatggat cagaaaggtc tgcgtgcgca
     3361 gtcactgaaa gagatcaaag gcgtgcagtg gatcccggac tggggccagg cgcgtatcga
     3421 gtcgatggtt gctaaccgtc ctgactggtg tatctcccgt cagcgcacct ggggcgtacc
     3481 gatgtcactg ttcgtgcaca aagacacgga agagctgcat ccgcgtactc tcgaactaat
     3541 ggaagaagtg gcaaaacgcg ttgaagttga cggcatccag gcgtggtggg atcttgatgc
     3601 gaaagagatc ctcggcgatg aagctgatca gtacgtgaaa gtgccggaca cattggatgt
     3661 atggtttgac tccggatcta cccactcttc tgttgttgac gtgcgtccgg aatttgccgg
     3721 tcacgcagcg gacatgtatc tggaaggttc tgaccagcac cgtggttggt tcatgtcttc
     3781 cctaatgatc tccaccgcga tgaaaggcaa agcgccgtat cgtcaggtac tgacccacgg
     3841 ctttaccgtg gatggtcagg gtcgcaagat gtctaaatcc atcggcaata ccgtttcgcc
     3901 gcaggatgtg atgaacaaac tgggcgcgga tattctgcgt ctgtgggtgg catcaaccga
     3961 ctacaccggt gaaatggccg tttctgacga gatcctgaaa cgtgctgccg atagctatcg
     4021 tcgtatccgt aacaccgcgc gcttcctgct ggcaaacctg aacggttttg atccagcaaa
     4081 agatatggtg aaaccggaag agatggtggt actggatcgc tgggccgtag gttgtgcgaa
     4141 agcggcacag gaagacatcc tcaaggcgta cgaagcatac gatttccacg aagtggtaca
     4201 gcgtctgatg cgcttctgct ccgttgagat gggttccttc tacctcgaca tcatcaaaga
     4261 ccgtcagtac accgccaaag cggacagtgt ggcgcgtcgt agctgccaga ctgcgctgta
     4321 tcacatcgca gaagcgctgg tgcgctggat ggcaccaatc ctctccttca ccgctgatga
     4381 agtgtggggc tacctgccgg gcgaacgtga aaaatacgtc ttcaccggtg agtggtacga
     4441 aggcctgttt ggcctggcag acagtgaagc gatgaacgat gcgttctggg acgagctgtt
     4501 gaaagtgcgt ggcgaagtga acaaagtcat tgagcaagcg cgtgccgaca agaaagtggg
     4561 tggctcgctg gaagcggcag taaccttgta tgcagaaccg gaactgtcgg cgaaactgac
     4621 cgcgctgggc gatgaattac gatttgtcct gttgacctcc ggcgctaccg ttgcagacta
     4681 taacgacgca cctgctgatg ctcagcagag cgaagtactc aaagggctga aagtcgcgtt
     4741 gagtaaagcc gaaggtgaga agtgcccacg ctgctggcac tacacccagg atgtcggcaa
     4801 ggtggcggaa cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtga
     4861 aaaacgtaag tttgcctgat gagtcaatcg atctgttcaa cagggctacg ctggctgtgg
     4921 ctggtggtag tcgtgctgat tatcgatctg ggcagcaaat acctgatcct ccagaacttt
     4981 gctctggggg atacggtccc gctgttcccg tcgcttaatc tgcattatgc gcgtaactat
     5041 ggcgcggcgt ttagtttcct tgccgatagc ggcggctggc agcgttggtt ctttgccggt
     5101 attgcgattg gtattagcgt gatcctggca gtgatgatgt atcgctcgaa ggccacgcag
     5161 aagctaaaca atatcgctta cgcgctgatt attggcggcg cgctgggcaa cctgttcgac
     5221 cgcctgtggc acggcttcgt tgtcgatatg atcgacttct acgtcggcga ctggcacttc
     5281 gccaccttca accttgccga tactgccatc tgtgtcggtg cggcactgat tgtgctggaa
     5341 ggttttttgc cttctagagc gaaaaaacaa taataaaccc tgccggatgc gatgctgacg
     5401 catcttatcc ggcctacaga ttgctgcgaa atcgtaggcc ggataaggcg tttacgccgc
     5461 atccggcaaa aatcctcaaa tataagagca aacctgcatg tctgaatctg tacagagcaa
     5521 tagcgccgtc ctggtgcact tcacgctaaa actcgacgat ggcaccaccg ccgagtctac
     5581 ccgcaacaac ggtaaaccgg cgctgttccg cctgggtgat gcttctcttt ctgaagggct
     5641 ggagcaacac ctgttggggc tgaaagtggg cgataaaacc accttctcgt tggagccaga
     5701 tgcggcgttt ggcgtgccgt caccggacct gattcagtac ttttcccgcc gtgaatttat
     5761 ggatgcaggc gagccagaaa ttggcgcaat catgcttttt accgcaatgg atggcagtga
     5821 gatgcctggc gtgatccgcg aaattaacgg cgactccatt accgttgatt tcaaccatcc
     5881 gctggccggg cagaccgttc attttgatat tgaagtgctg gaaatcgatc cggcattgga
     5941 ggcgtaacat gcagatcctg ttggccaacc cgcgtggttt ttgtgccggg gtagaccgcg
     6001 ctatcagcat tgttgaaaac gcgctggcca tttacggcgc accgatatat gtccgtcacg
     6061 aagtggtaca taaccgctat gtggtcgata gcttgcgtga gcgtggggct atctttattg
     6121 agcagattag cgaagtaccg gacggcgcga tcctgatttt ctccgcacac ggtgtttctc
     6181 aggcggtacg taacgaagca aaaagccgcg atttgacggt gtttgatgcc acctgtccgc
     6241 tggtgaccaa agtgcatatg gaagtcgccc gcgccagccg tcgtggcgaa gagtcgattc
     6301 tcatcggcca cgccgggcat ccggaagtgg aaggtacaat gggccagtac agtaacccgg
     6361 aagggggaat gtatctggtc gaatcgccgg acgatgtgtg gaaactgacg gtcaaaaacg
     6421 aagagaagct ctcctttatg acccagacca cgctgtcggt ggatgacacg tctgatgtga
     6481 tcgacgcgct gcgtaaacgc ttcccgaaaa ttgtcggtcc gcgcaaagat gacatctgct
     6541 acgccacgac taaccgtcag gaagcggtac gcgccctggc agaacaggcg gaagttgtgt
     6601 tggtggtcgg ttcgaaaaac tcctccaact ccaaccgtct ggcggagctg gcccagcgta
     6661 tgggcaaacg cgcgtttttg attgacgatg cgaaagatat ccaggaagag tgggtgaaag
     6721 aggttaaatg cgtcggcgtg actgcgggcg catcggctcc ggatattctg gtgcaaaatg
     6781 tggtggcacg tttgcagcag ctgggcggtg gtgaagccat tccgctggaa ggccgtgaag
     6841 aaaacattgt tttcgaagtg ccgaaagagc tgcgtgtcga tattcgtgaa gtcgattaag
     6901 tcattagcag cctaagttat gcgaaaatgc cggtcttgtt accggcattt tttatggaga
     6961 aaacatgcgt ttacctatct tcctcgatac tgaccccggc attgacgatg ccgtcgccat
     7021 tgccgccgcg atttttgccc ccgaactcga cctgcaactg atgaccaccg tcgcgggtaa
     7081 tgtctcggtt gagaaaacca cccgcaacgc cctgcaactg ctgcatttct ggaatgcgga
     7141 gattccgctc gcccagggag ccgctgtgcc gctggtacgc gcaccgcgtg atgcagcatc
     7201 tgtgcacggc gaatcgggaa tggctggcta cgactttgtt gagcacaacc gaaagccaat
     7261 cgggatacca gcgtttctgg cgattcgtga tgccctgatg cgtgcaccag agcctgttac
     7321 cctggtggcc attggcccgt taaccaatat tgcgctgtta ctttcacaat gcccggaatg
     7381 taaaccgtat attcgtcgtc tggtgatcat gggcggttct tctggtcgcg gcaactgtac
     7441 accaaatgcc gagtttaata tcgctgccga tccagaagcg gctgcctgtg tcttccgtag
     7501 cggaatcgaa atcgtcatgt gcggtctgga tgtcaccaat caggcaatat taacccctga
     7561 ctatctcgct actttgccgg agttgaatcg caccggaaaa atgcttcatg cactgtttag
     7621 ccactaccgt agcggcagta tgcaaagcgg tctgcgaatg cacgatctct gcgccatcgc
     7681 ctggctggtg cgcccggact tattcaccct caagccgtgt tttgtcgcgg tagaaactca
     7741 aggcgaattt acctcaggca cgacggtggt tgatatcgac ggttgcctgg gcaaaccagc
     7801 caatgtaaag gtggcattgg atctggatgt gaaaggcttc cagcagtggg tggctgaggt
     7861 gctggctctg gcgtcgtaac ctgtcacatg ttatcggcat gcagtcattc atcgactcat
     7921 gcctttcact gatatccctc cctgtttatc attaatttct aattatcagc gtttttggct
     7981 ggcggcgtag cgatgcgctg gttactctga aaacggtcta tgcaaattaa caaaagagaa
     8041 tagctatgca tgatgcaaac atccgcgttg ccatcgcggg agccgggggg cgtatgggcc
     8101 gccagttgat tcaggcggcg ctggcattag aaggcgtgca gctgggcgcg gcgctggagc
     8161 gcgaaggatc ttctttactg ggcagcgacg ccggtgagct ggctggtgca gggaaaacag
     8221 gcgttaccgt gcaaagcagc ctcgatgcgg taaaagatga ttttgatgtg tttatcgatt
     8281 ttacccgtcc ggaaggtacg ctgaaccatc tcgctttttg tcgccagcat ggcaaaggga
     8341 tggtgatcgg cactacgggg tttgacgaag ccggtaaaca ggcgattcgt gacgccgccg
     8401 ccgatattgc gattgtcttt gccgccaatt ttagcgttgg cgttaacgtc atgcttaagc
     8461 tgctggagaa agcagccaaa gtgatgggtg actacaccga tatcgaaatt attgaagcac
     8521 atcatagaca taaagttgat gcgccgtcag gcaccgcact ggcaatggga gaggcgatcg
     8581 cccacgccct tgataaagat ctgaaagatt gcgcggtcta cagtcgtgaa ggccacaccg
     8641 gtgaacgtgt gcctggcacc attggttttg ccaccgtgcg tgcaggtgac atcgttggtg
     8701 aacataccgc gatgtttgcc gatattggcg agcgtctgga gatcacccat aaggcgtcca
     8761 gccgtatgac atttgctaac ggcgcggtaa gatcggcttt gtggttgagt ggtaaggaaa
     8821 atggtctttt tgatatgcga gatgtactta atctcaataa tttgtaacca caaaatattt
     8881 gttatggtgc aaaaataaca catttaattt attgattata aagggcttta atttttggcc
     8941 cttttatttt tagtgttatg tttttaaata ttctataagt gccaaaaatt acatgttttg
     9001 tcttctattt ttgttgtttt aatgtaaatt ttgaccattt ggtccacttt ttcctgctcg
     9061 tttttatttc atgcaatctt cttgctgcgc aagcgttttc cagaacaggt tatatgatct
     9121 ttttgtcgct taatgcctgt aaaatatgca tgagccacaa aataatataa aaaataccgc
     9181 cattaagttg acttttagcg cccatatctc cagaatgccg ccgtttgcca gaaattcgtc
     9241 ggtaagcaga tttgcattga tttacgtcat cattgtgaat taatatgcaa ataaagtgag
     9301 tgaatattct ctggagggtg ttttgattaa gtcagcgcta ttggttctgg aagacggaac
     9361 ccagtttcac ggtcgggcca taggggcaac aggttcggcg gttggggaag tcgttttcaa
     9421 tacttcaatg accggttatc aagaaatcct cactgatcct tcctattctc gtcaaatcgt
     9481 tactcttact tatccccata ttggcaatgt cggcactaat gacgccgatg aagaatcttc
     9541 tcaggtacat gcacaaggtc tggtgattcg cgatctgccg ctgattgcca gcaacttccg
     9601 taataccgaa gacctctctt cttacctgaa gcgccataac atcgtggcga ttgccgatat
     9661 cgatacccgt aagctgacgc gtttactgcg cgagaaaggc gcacagaatg gctgcattat
     9721 cgcaggcgat aacccggatg cggcgttggc gttagaaaaa gcccgcgcgt tcccaggtct
     9781 gaatggcatg gatctggcaa aagaagtgac caccgcagaa gcctatagct ggacacaagg
     9841 gagctggacg ttgaccggcg gcctgccaga agcgaaaaaa gaagacgagc tgccgttcca
     9901 cgtcgtggct tatgattttg gtgccaagcg caacatcctg cggatgctgg tggatagagg
     9961 ctgtcgcctg accatcgttc cggcgcaaac ttctgcggaa gatgtgctga aaatgaatcc
    10021 agacggcatc ttcctctcca acggtcctgg cgacccggcc ccgtgcgatt acgccattac
    10081 cgccatccag aaattcctcg aaaccgatat tccggtattc ggcatctgcc tcggtcatca
    10141 gctgctggcg ctggcgagcg gtgcgaagac tgtcaaaatg aaatttggtc accacggcgg
    10201 caaccatccg gttaaagatg tggagaaaaa cgtggtgatg atcaccgccc agaaccacgg
    10261 ttttgcggtg gatgaagcaa cattacctgc aaacctgcgt gtcacgcata aatccctgtt
    10321 cgacggtacg ttacagggca ttcatcgcac tgataaaccg gcgttcagct tccagggtca
    10381 cccggaagcc agccctgggc cacacgacgc agcgccgttg ttcgaccact ttatcgagtt
    10441 aattgagcag taccgtaaaa ccgctaagta atcaggagta aaagagccat gccaaaacgt
    10501 acagatataa aaagtatcct gattctgggt gcgggcccga ttgttatcgg tcaggcgtgt
    10561 gagtttgact actctggcgc gcaagcatgt aaagccctgc gcgaagaggg ttaccgcgtc
    10621 attctggtga actccaaccc ggcgaccatc atgaccgacc cggaaatggc cgatgcgacc
    10681 tacatcgagc cgattcactg ggaagtagta cgcaagatta ttgaaaaaga gcgcccggac
    10741 gcggtgctgc caacgatggg cggtcagacg gcgctgaact gcgcgctgga gctggagcgt
    10801 cagggcgtgt tggaagagtt cggcgtgact atgattggtg cgaccgccga tgcgattgat
    10861 aaagcagaag accgccgtcg tttcgacgta gcgatgaaga aaattggtct ggaaaccgcg
    10921 cgttccggta tcgcacacac gatggaagaa gcgctggcgg ttgccgctga cgtgggcttc
    10981 ccgtgcatta ttcgcccatc ctttaccatg ggcggtagcg gcggcggtat cgcttataac
    11041 cgcgaagagt ttgaagaaat ttgcgcccgc ggtctggatc tctccccaac caaagagctg
    11101 ctgattgatg agtcgctgat cggctggaaa gagtacgaga tggaagtggt gcgtgataaa
    11161 aacgacaact gcatcatcgt ctgctctatc gaaaactttg atgcgatggg catccatacc
    11221 ggtgactcca tcactgtcgc gccagcccaa acgctgaccg acaaagaata tcaaatcatg
    11281 cgtaacgcct cgatggcggt gctgcgtgaa atcggcgttg aaaccggtgg ttccaatgtc
    11341 cagtttgcgg tgaacccgaa aaacggtcgc ctgattgtta tcgaaatgaa cccacgcgtg
    11401 tcccgttctt cggcgctggc gtcgaaagcg accggtttcc cgattgctaa agtggcggcg
    11461 aaactggcgg tgggttacac cctcgacgaa ctgatgaacg acatcactgg cggacgtact
    11521 ccggcctcct tcgagccgtc catcgactat gtggttacta aaattcctcg cttcaacttc
    11581 gaaaaattcg ctggtgctaa cgaccgtctg accactcaga tgaaatcggt tggcgaagtg
    11641 atggcgattg gtcgcacgca gcaggaatcc ctgcaaaaag cgctgcgcgg cctggaagtc
    11701 ggtgcgactg gattcgaccc gaaagtgagc ctcgatgacc cggaagcgtt aaccaaaatc
    11761 cgtcgcgaac tgaaagatgc tggcgcagag cgtatctggt acatcgccga tgccttccgt
    11821 gcgggcctgt ctgtggacgg cgtgttcaac ctgaccaata ttgaccgctg gttcctggta
    11881 cagattgaag aactggtgcg tctggaagag aaagtagcgg aagtgggcat cactggcctg
    11941 aacgctgaat tcctgcgcca gctgaaacgc aaaggctttg ccgatgcgcg tctggcaaaa
    12001 ctggcgggcg tgcgcgaagc ggaaatccgc aagctgcgcg accagtatga cctgcacccg
    12061 gtttataagc gcgtggatac ctgtgcggca gagttcgcca ccgacaccgc ttacatgtac
    12121 tccacttatg aagaagagtg cgaagcgaat ccgtctaccg accgtgaaaa aatcatggtt
    12181 ctcggcggcg gcccgaaccg tatcggtcag ggtatcgaat tcgactactg ctgtgtacac
    12241 gcctcgctgg cgctgcgcga agacggttac gaaaccatta tggttaactg taacccggaa
    12301 accgtctcca ccgactacga cacttccgat cgcctctact tcgagccggt aactctggaa
    12361 gatgtgctgg aaatcgtgcg tattgagaag ccgaaaggcg ttatcgttca gtacggcggt
    12421 cagaccccgc tgaaactggc gcgcgcgctg gaagctgctg gcgtaccggt tatcggcacc
    12481 agcccggatg ctatcgaccg tgcagaagac cgtgaacgct tccagcatgc ggttgagcgt
    12541 ctgaaactga aacaaccggc gaacgccacc gttaccgcta ttgaaatggc ggtagagaag
    12601 gcgaaagaga ttggctaccc actggtggta cgtccgtctt acgttctcgg cggtcgggcg
    12661 atggaaatcg tctatgacga agctgacctg cgtcgctact tccagacggc ggtcagcgtg
    12721 tctaacgatg cgccagtgtt gctggatcac ttccttgatg acgcggtaga agttgacgtg
    12781 gatgccatct gcgacggcga aatggtgctg attggcggca tcatggagca catcgagcag
    12841 gcgggcgtgc actccggtga ctccgcatgt tctctgccag cctacacctt aagtcaggaa
    12901 attcaggatg tgatgcgcca gcaggtgcag aaactggcct tcgaattgca ggtgcgcggt
    12961 ctgatgaacg tgcagtttgc ggtgaaaaac aacgaagtct acctgattga agttaacccg
    13021 cgtgcggcgc gtaccgttcc gttcgtctcc aaagccaccg gcgtaccgct ggcaaaagtg
    13081 gcggcgcgtg tgatggctgg caaatcgctg gctgagcagg gcgtaaccaa agaagttatc
    13141 ccgccgtact actcggtgaa agaagtggtg ctgccgttca ataaattccc gggtgttgac
    13201 ccgctgttag ggccagaaat gcgctccacc ggggaagtga tgggcgtggg ccgcaccttc
    13261 gctgaagcgt ttgccaaagc gcagctgggc agcaactcca ccatgaagaa acacggtcgt
    13321 gcgttgcttt ccgtgcgcga aggcgataaa gaacgcgtgg tggacctggc ggcaaaactg
    13381 ctgaaacagg gcttcgagct ggatgcgacc cacggcacgg cgattgtgct gggcgaagcg
    13441 ggcattaatc cgcgtctggt gaacaaggtg catgaaggcc gtccgcacat tcaggaccgc
    13501 atcaagaatg gcgaatatac ctacatcatc aacaccactt caggccgtcg cgcgattgaa
    13561 gactcccgcg tgatccgtcg cagtgcgctg caatataaag tgcattatga caccaccctg
    13621 aacggtggtt tcgctaccgc gatggcgctg aatgccgacg cgaccgaaaa agtaatttcg
    13681 gtgcaggaaa tgcacgcgca gatcaaataa tagcgtgtca tggcggatat ttttcatccg
    13741 ctaatttgat cgaataagta atacggttct ctgatgagga ccgttttttt tgcccattaa
    13801 ttaaatcttt tggggaatcg atatttttga tgacataagc aggatttagc tcaca
//
LOCUS       DAEEBB010000112        13807 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEEBB010000112 DAEEBB010000000
VERSION     DAEEBB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.112"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="KLF07_003082"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="KLF07_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBD9833036.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="KLF07_003083"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="KLF07_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBD9833037.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="KLF07_003084"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="KLF07_003084"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBD9833038.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="KLF07_003085"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="KLF07_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBD9833039.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="KLF07_003086"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="KLF07_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="KLF07_003087"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="KLF07_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBD9833040.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="KLF07_003088"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="KLF07_003088"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBD9833041.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="KLF07_003089"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="KLF07_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBD9833042.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="KLF07_003090"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="KLF07_003090"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBD9833043.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="KLF07_003091"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="KLF07_003091"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBD9833044.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="KLF07_003092"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="KLF07_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="KLF07_003093"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="KLF07_003093"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBD9833045.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="KLF07_003094"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="KLF07_003094"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBD9833046.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="KLF07_003095"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="KLF07_003095"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBD9833047.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAEEBB010000113        13796 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEEBB010000113 DAEEBB010000000
VERSION     DAEEBB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.113"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..147)
                     /locus_tag="KLF07_003096"
     CDS             complement(<1..147)
                     /locus_tag="KLF07_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBD9833048.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="KLF07_003097"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="KLF07_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="KLF07_003098"
                     /pseudo
     CDS             476..1021
                     /locus_tag="KLF07_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="KLF07_003099"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="KLF07_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD9833049.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="KLF07_003100"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="KLF07_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBD9833050.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="KLF07_003101"
     CDS             3028..3201
                     /locus_tag="KLF07_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833051.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="KLF07_003102"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="KLF07_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBD9833052.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="KLF07_003103"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="KLF07_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBD9833053.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="KLF07_003104"
     CDS             complement(5499..6422)
                     /locus_tag="KLF07_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9833054.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="KLF07_003105"
     CDS             6639..7982
                     /locus_tag="KLF07_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD9833055.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="KLF07_003106"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="KLF07_003106"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBD9833056.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="KLF07_003107"
     CDS             10002..10226
                     /locus_tag="KLF07_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBD9833057.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="KLF07_003108"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="KLF07_003108"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBD9833058.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="KLF07_003109"
     CDS             complement(10820..11800)
                     /locus_tag="KLF07_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBD9833059.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="KLF07_003110"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="KLF07_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBD9833060.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="KLF07_003111"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="KLF07_003111"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBD9833061.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3576 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgttggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catctc
//
LOCUS       DAEEBB010000114        13320 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEEBB010000114 DAEEBB010000000
VERSION     DAEEBB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.114"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="KLF07_003112"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="KLF07_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBD9833062.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="KLF07_003113"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="KLF07_003113"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD9833063.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="KLF07_003114"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="KLF07_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBD9833064.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="KLF07_003115"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="KLF07_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBD9833065.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="KLF07_003116"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="KLF07_003116"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBD9833066.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="KLF07_003117"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="KLF07_003117"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBD9833067.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="KLF07_003118"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="KLF07_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBD9833068.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="KLF07_003119"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="KLF07_003119"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBD9833069.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="KLF07_003120"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="KLF07_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBD9833070.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="KLF07_003121"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="KLF07_003121"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD9833071.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /locus_tag="KLF07_003122"
     CDS             11095..11487
                     /locus_tag="KLF07_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBD9833072.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12299
                     /locus_tag="KLF07_003123"
                     /pseudo
     CDS             11491..12299
                     /locus_tag="KLF07_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12335..13189
                     /gene="kdsA"
                     /locus_tag="KLF07_003124"
     CDS             12335..13189
                     /gene="kdsA"
                     /locus_tag="KLF07_003124"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBD9833073.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3353 a         3307 c         3413 g         3247 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggctggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacaggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc
    11821 gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat
    11881 gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata
    11941 tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc
    12001 ttgatgaagc tgataacatt gaggtaatcc gcaaattgtt ggatacactc aaagcctcgt
    12061 tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca
    12121 acccggaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg
    12181 aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca
    12241 gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat
    12301 taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg
    12361 cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggtggtatga acgtgctgga
    12421 atctcgcgat ctggcgatgc gcatttgcga gcactacgta accgtgaccc agaaactggg
    12481 tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta
    12541 tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg
    12601 cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt
    12661 ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc
    12721 gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg
    12781 taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg
    12841 tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa
    12901 agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc
    12961 gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat
    13021 ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg
    13081 tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc
    13141 gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct
    13201 tgaaaaataa agccttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa
    13261 aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc
//
LOCUS       DAEEBB010000115        13260 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEEBB010000115 DAEEBB010000000
VERSION     DAEEBB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.115"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="KLF07_003125"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="KLF07_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBD9833074.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="KLF07_003126"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="KLF07_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBD9833075.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="KLF07_003127"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="KLF07_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBD9833076.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="KLF07_003128"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="KLF07_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBD9833077.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="KLF07_003129"
     CDS             complement(4036..5568)
                     /locus_tag="KLF07_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBD9833078.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="KLF07_003130"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="KLF07_003130"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBD9833079.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="KLF07_003131"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="KLF07_003131"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBD9833080.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="KLF07_003132"
     CDS             complement(8663..10399)
                     /locus_tag="KLF07_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBD9833081.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="KLF07_003133"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="KLF07_003133"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBD9833082.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="KLF07_003134"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="KLF07_003134"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBD9833083.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="KLF07_003135"
     CDS             complement(12121..12855)
                     /locus_tag="KLF07_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBD9833084.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="KLF07_003136"
     CDS             13056..>13260
                     /locus_tag="KLF07_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBD9833085.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3366 a         3370 c         3488 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAEEBB010000116        13210 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEEBB010000116 DAEEBB010000000
VERSION     DAEEBB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.116"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..807
                     /locus_tag="KLF07_003137"
     CDS             <1..807
                     /locus_tag="KLF07_003137"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD9833086.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="KLF07_003138"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="KLF07_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBD9833087.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="KLF07_003139"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="KLF07_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBD9833088.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /locus_tag="KLF07_003140"
     CDS             complement(3460..6573)
                     /locus_tag="KLF07_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBD9833089.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="KLF07_003141"
     CDS             complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="KLF07_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBD9833090.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="KLF07_003142"
     CDS             7815..8093
                     /locus_tag="KLF07_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833091.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="KLF07_003143"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="KLF07_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBD9833092.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="KLF07_003144"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="KLF07_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBD9833093.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="KLF07_003145"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="KLF07_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBD9833094.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="KLF07_003146"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="KLF07_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBD9833095.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="KLF07_003147"
     CDS             11085..11732
                     /locus_tag="KLF07_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBD9833096.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="KLF07_003148"
     CDS             complement(11774..12304)
                     /locus_tag="KLF07_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9833097.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="KLF07_003149"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="KLF07_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBD9833098.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2982 c         3030 g         3599 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc
//
LOCUS       DAEEBB010000117        12998 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEEBB010000117 DAEEBB010000000
VERSION     DAEEBB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.117"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            98..1393
                     /locus_tag="KLF07_003150"
     CDS             98..1393
                     /locus_tag="KLF07_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBD9833099.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="KLF07_003151"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="KLF07_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBD9833100.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="KLF07_003152"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="KLF07_003152"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD9833101.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="KLF07_003153"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="KLF07_003153"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBD9833102.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="KLF07_003154"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="KLF07_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBD9833103.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="KLF07_003155"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="KLF07_003155"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBD9833104.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="KLF07_003156"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="KLF07_003156"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBD9833105.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="KLF07_003157"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="KLF07_003157"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBD9833106.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAEEBB010000118        12872 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEEBB010000118 DAEEBB010000000
VERSION     DAEEBB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.118"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..696)
                     /locus_tag="KLF07_003158"
     CDS             complement(<1..696)
                     /locus_tag="KLF07_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD9833107.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="KLF07_003159"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="KLF07_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBD9833108.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="KLF07_003160"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="KLF07_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBD9833109.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="KLF07_003161"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="KLF07_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBD9833110.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="KLF07_003162"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="KLF07_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBD9833111.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="KLF07_003163"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="KLF07_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBD9833112.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="KLF07_003164"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="KLF07_003164"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBD9833113.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="KLF07_003165"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="KLF07_003165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBD9833114.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="KLF07_003166"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="KLF07_003166"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBD9833115.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="KLF07_003167"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="KLF07_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBD9833116.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="KLF07_003168"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="KLF07_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBD9833117.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="KLF07_003169"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="KLF07_003169"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBD9833118.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="KLF07_003170"
     CDS             10119..10499
                     /locus_tag="KLF07_003170"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBD9833119.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="KLF07_003171"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="KLF07_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBD9833120.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="KLF07_003172"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="KLF07_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBD9833121.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="KLF07_003173"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="KLF07_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         3279 a         3147 c         3353 g         3093 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggaactc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gc
//
LOCUS       DAEEBB010000119        12359 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEEBB010000119 DAEEBB010000000
VERSION     DAEEBB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.119"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(121..330)
                     /locus_tag="KLF07_003174"
                     /pseudo
     CDS             complement(121..330)
                     /locus_tag="KLF07_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="KLF07_003175"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="KLF07_003175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBD9833122.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="KLF07_003176"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="KLF07_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBD9833123.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="KLF07_003177"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="KLF07_003177"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBD9833124.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="KLF07_003178"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="KLF07_003178"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBD9833125.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="KLF07_003179"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="KLF07_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBD9833126.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="KLF07_003180"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="KLF07_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBD9833127.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="KLF07_003181"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="KLF07_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBD9833128.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="KLF07_003182"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="KLF07_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBD9833129.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="KLF07_003183"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="KLF07_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBD9833130.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="KLF07_003184"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="KLF07_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBD9833131.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="KLF07_003185"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="KLF07_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBD9833132.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="KLF07_003186"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="KLF07_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBD9833133.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAEEBB010000120        12039 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEEBB010000120 DAEEBB010000000
VERSION     DAEEBB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.120"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..185)
                     /locus_tag="KLF07_003187"
     CDS             complement(<1..185)
                     /locus_tag="KLF07_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBD9833134.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="KLF07_003188"
     CDS             complement(230..1216)
                     /locus_tag="KLF07_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBD9833135.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            complement(1527..2663)
                     /gene="hemW"
                     /locus_tag="KLF07_003189"
     CDS             complement(1527..2663)
                     /gene="hemW"
                     /locus_tag="KLF07_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBD9833136.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2656..3249)
                     /locus_tag="KLF07_003190"
     CDS             complement(2656..3249)
                     /locus_tag="KLF07_003190"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBD9833137.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3257..3547)
                     /locus_tag="KLF07_003191"
     CDS             complement(3257..3547)
                     /locus_tag="KLF07_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBD9833138.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            complement(3544..4110)
                     /gene="yggT"
                     /locus_tag="KLF07_003192"
     CDS             complement(3544..4110)
                     /gene="yggT"
                     /locus_tag="KLF07_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBD9833139.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4128..4832)
                     /gene="yggS"
                     /locus_tag="KLF07_003193"
     CDS             complement(4128..4832)
                     /gene="yggS"
                     /locus_tag="KLF07_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBD9833140.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            4850..5830
                     /locus_tag="KLF07_003194"
     CDS             4850..5830
                     /locus_tag="KLF07_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBD9833141.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(6005..6421)
                     /gene="ruvX"
                     /locus_tag="KLF07_003195"
     CDS             complement(6005..6421)
                     /gene="ruvX"
                     /locus_tag="KLF07_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBD9833142.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6421..6984)
                     /locus_tag="KLF07_003196"
     CDS             complement(6421..6984)
                     /locus_tag="KLF07_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBD9833143.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7093..8043)
                     /gene="gshB"
                     /locus_tag="KLF07_003197"
     CDS             complement(7093..8043)
                     /gene="gshB"
                     /locus_tag="KLF07_003197"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBD9833144.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8056..8787)
                     /gene="rsmE"
                     /locus_tag="KLF07_003198"
     CDS             complement(8056..8787)
                     /gene="rsmE"
                     /locus_tag="KLF07_003198"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBD9833145.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(8867..9574)
                     /gene="endA"
                     /locus_tag="KLF07_003199"
     CDS             complement(8867..9574)
                     /gene="endA"
                     /locus_tag="KLF07_003199"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBD9833146.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9669..10166)
                     /locus_tag="KLF07_003200"
     CDS             complement(9669..10166)
                     /locus_tag="KLF07_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBD9833147.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(10243..11637)
                     /gene="galP"
                     /locus_tag="KLF07_003201"
     CDS             complement(10243..11637)
                     /gene="galP"
                     /locus_tag="KLF07_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBD9833148.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         2950 a         3079 c         3070 g         2940 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaaccta tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1381 gtttataatc aataaatgat gagtaaatat tcactagata tatctaataa atatcatcga
     1441 tgtttattca gtaagaaata tattttgccg aagttaatta acggggggga aagaagtgat
     1501 taatgcgccc gcaggcgcat taatgattac tcagcgagga ataattcaag aagtgagttt
     1561 aagaataatt taccgtgctc ggttatctgc cagtaatccg cacattcggt gagataaccc
     1621 tgggcaatcg cctcgtctaa ctgtgggcga atcacatctt cgcaaagccc ggtatacgca
     1681 ataaactcca cgcgcggcgc ggcctccagc agacggaagc gattcataaa gaactcaaac
     1741 ggcttatctg cggcttcgac atcacgctgg ctttccagat accttccttg cataaaacca
     1801 cgaggatgac gcgttttagt ggtacgcaga atgcgcccat ccgggaaggt gactttgccg
     1861 tgcgcgccgc agccaatacc gatgtagtca ccaaatcgcc agtagttgag attgtgctgg
     1921 cactgataac cgggtttggc gtaagcggaa gtttcatatt gctgataacc cgctgcggtt
     1981 aataactgat gcccctgttc gaaaatatcc cacagcgcgt catcgtccgg cagcaccggt
     2041 ggtcgcgaac caaacagcgt attgggttcg atggtcagtt gataccagga aagatgcggc
     2101 ggattcagtt caatggcctg gcgtagatcg ccaagcgcct cttccagtga ttgatccggt
     2161 agtccatgca tcaaatcgag gttaaagcta cgcagcccca gcccgctcgc cagctttgcc
     2221 gcgcgtttcg cttcttgcgg gccatgaata cgcccaagtc gtttcagctt ttcttcgcta
     2281 aaactctgca caccaataga gatgcggttc acaccagcac gctgataatc gacaaagcga
     2341 tcggcttcta ccgttccagg gttcgcttcc atagtaattt ctgcatccgc tgccagcggc
     2401 aaacgcgcac gcacgccgtc cagcagcgtt tgcatcgccg ggccggaaag caggctcggc
     2461 gtaccaccgc caataaagat tgtctttact tcacggccct gagcgtaagc cacatcattg
     2521 tccagatcgc aaagcagatg ctgaacataa tcgtcgtgcg gcacttctcc tttcaacgcg
     2581 tgagagttga aatcgcagta cgggcatttc tgcacgcacc acgggatgtg aatgtagaga
     2641 ctcagcggcg gtaatttaac cattacgtaa agcgtccagc agcagtttca acgcctgacc
     2701 acggtgggaa atggcgctct tttcttcgcg ggtcagttcg gcggcggttt tcccttcgga
     2761 aggtacgaag aagattggat cataaccaaa gccaccagtg cccgctggtt cgcgagtaat
     2821 cacgcccggc cagctgccgt ggcataccag cggagtcgga tcttccgcgt gacgcagata
     2881 taccagcacg cagtggaaac gcgcctgacg ttggtcgtcc ggtacgtctt tcagtgtttc
     2941 caacagtttt tgcagattct tttgatcggt cgcgtcttca ccggaataac gcgcggagta
     3001 aatacccggc gcgccaccca gcgcatctac cgccagacca gagtcgtcgg caattgccgg
     3061 taaaccggtc acttttgccg catagcgcgc tttcagaatc gcgttttcga taaaggtcag
     3121 gccggtttct tcagcggaat ctacgccgag gtctgtttgg gccacgatat caagaccgaa
     3181 gtcgctaagc agcgacgcca gctcacgcac tttaccggca ttgccggttg cgaggacaac
     3241 tttttgcata ggatacctaa ttaattaacg ccacgatttc tggcgggatt tgttgcggat
     3301 taatgatttt aatttgtttg tggcggccaa gttcgccttt ttcaatcacc acctggcttt
     3361 tggcaacccg gaattgctta ccgagaaact tcaccagatg actgttgccc tggccgtcaa
     3421 ccggcggcgc ggtaatggcg actttaactt cgtcgccatg taaaccgaca atagaatcac
     3481 ggctggcttt cggctgaata tagagccgta aaaccagacc gtcatcatta actgttacgg
     3541 cactcataac gccatccaca gccccggcag cagcatgttt ccggttgcct gtaatacttc
     3601 tgcgacaccc atattgatga catacagcag cagaacgagg atcatcggcg agaaatcaat
     3661 cccacccatt gccggtagca ggcggcgaat cgggcgcagc agcggatcgg ccagctgaat
     3721 cagcacgtat tcaatcggac tacgaccctg gcttacccag ctcataatcg ccatcaccag
     3781 caggacccag aaaatcagca ggccgatggt tttcagcaga atcagtaaac cagcaatcca
     3841 gatgattggc aggaaggtca ccactttaaa cagcacgatg gctttgataa aactgagaat
     3901 ataggcaacc agcagcgagg cgctgtcaat ggggcccatt gccggaataa cgcggcgcag
     3961 tggcccgata attggctgcg ttaccttcac tacaaactgt gagaaggggt tgtaaaaatc
     4021 acaatgagcc cactgcatcc agatgcgtaa taacagcacc atggtataca gctcaatgac
     4081 cgttgaaagc aggaaagtca acgtattcat ggcgttcctt taattcctta ttttttagag
     4141 taatcacgcg caccaaaaat tgcagtgccg atacgaacca tcgtgctacc tgccgcaatg
     4201 gcggcttcca tatcgtccga cattcccaga gagagcgtgt cgatatgcgg gtagcgcgtt
     4261 ttcagtccgg caaatgctac agccatttgg cgtgcaactt caaactgcct tacatattct
     4321 gactcaggcg cagggattgc catcagcccg cgcagacgta aacgcggtag ttcagcgacc
     4381 gccgctgcca gctcgtccag ttctgccagt tgaatcccgg acttactgtt ttcatcacta
     4441 atattaattt gaatcagtac gttaagaggg ggaagttctg ccgggcgctg atcgttgagg
     4501 cgggtagcaa tgcgcaaacg gtcgatggta tgacaccagt cgaaatgctc tgccaccagg
     4561 cggcttttat tagactgcaa cgggccaata aaatgccatt ctaatcctgt tacgcccagt
     4621 tcctgaaagt ggcgaatttt atctacccct tcctgaacgt agttttcacc aaattgacgc
     4681 tgcccggcat caatggcttc tgcgatggcg ctcgcaggtt ttgttttact gactgcaagc
     4741 agcgtaattt cttctggaga acggccgcaa cgcgttgcag ccgctgagat tttgtcccgg
     4801 acctgtgcca ggttatgcgc aatatcgttc attttccgag gatcttagta tgaatatgga
     4861 agaaattgtg gcccttagtg taaagcataa cgtctcggat ctacacctgt gcagcgcctg
     4921 gcccgcacga tggcgcattc gcgggctaat ggaagctgcg ccgtttgagg cgccggacgt
     4981 cgaagagcta ctgcgggagt ggctggatga cgatcagcgg gcaatattgc tggaaaatgg
     5041 ccagctggat tttgccgtgt cgctggcgga aaaccagcga ttgcgtggca gcgcatttgc
     5101 gcaacggcat ggcatttcgc tggcgttacg gctgttacct tcgcactgcc cgcagctcga
     5161 acagcttggc gcaccaacgg tattgccgga attactcaag agtgagaatg gcctgattct
     5221 ggtgacgggg gcgacgggga gcagcaaatc taccacgctg gcggcgatgg ttggctatct
     5281 caatcaacat gccgatgcgc atattctgac gctggaagat cctgttgaat atatctatgc
     5341 cagccagcga tgtttgatcc agaagcggga aattggtttg cactgtatga cgttcgcatc
     5401 gggattgcgg gccgcattgc gggaagatcc tgatgtgatt ttgctcggag agctgcgtga
     5461 cagcgagaca atccgtctgg cactgacggc ggcagaaacc gggcatttgg tgctggcaac
     5521 attacatacg cgtggtgccg cgcaggcagt tgagcgactg gtggattcat ttccggcgca
     5581 ggaaaaagat cccgtacgta atcaactggc aggtagttta cgggccgtgt tgtcacaaaa
     5641 gctggaagtg gataaacagg aaggacgcgt ggcgctgttt gaattattga ttaacactcc
     5701 cgcggtgggg aatttgattc gcgaagggaa aacccaccag ttaccgcatg ttattcaaac
     5761 cgggcagcag gtggggatga taacgtttca gcagagtttt cagcagcgag tgggggaagg
     5821 gcgtttgtga cgttgccatg cccgatgtgc aagcttatcg ggcctacaaa ggcaataaac
     5881 gtctgatgag acgtttgcgc gtcttatcag acctacaatc gctgccacat gtaggccgga
     5941 taggcgttca cgccgcatcc ggcaagatgg cggtaacgac tatccgccag gcttttaaat
     6001 cgccttaata tccctgctcg aaatagcttt cgagaataat aaccgcagag gcagagtcga
     6061 ctttgccttt gttgagcgcc cgatagccgc cctgttcaaa cagaccggaa cgggcttcca
     6121 cggtgctaag acgttcgtca tggagcttta cttcaacacc gaaacgacca tgaatacggt
     6181 tggcaaattt acgcgctctg gcagtcaatg gttgctcggt gccgtccata ttcagcggca
     6241 aaccgacgat gatttcgtcc ggctgccact ctttcagtaa acgttcgatg aggttccagt
     6301 ccggcgtacc gtcctgtgct ttaattgcag gcaaagggcg agcagtgccg gtaatgcgtt
     6361 ggccgaccgc tacgccaatg cttttggtgc cgaagtcgaa ggcgagtaag gttccactca
     6421 tcaggcgtgc cctgccacac caggcatggt gagaatatcc acaccaatca gttttgccgc
     6481 ctcgcgccag cggtcggcaa tcggcgtttt gaacagaata ttcagatccg ccggggccgt
     6541 tagccacgcg ttatcgagaa tttcttgttc cagttgacct ttttcccagg aggcataacc
     6601 tagagccacc aatacgtcag acggttgttt atcggtgccg agcgtttcca gcacatcgcg
     6661 ggaagtggtc atcactgtgt tgtctgaaat gcgaatgctg gaagcaaaat tggagggcgg
     6721 agtatgcaaa ataaacccgc gatcttcagc cagcggaccg ccgagcataa ccggtttatc
     6781 cagacggatt gattcatcac gcggctccgg cgtgatcttc agcttttcca gaatcccttc
     6841 aattttgaga ttttccagcg gcttgttgac gatgatcccc attgcaccat tggtattatg
     6901 ttcgcaaatg tagaccacgg aacgacggaa aatcggatcc tggagagcag gcatggcaat
     6961 aagaaagtga tgctgtaaat tcattgtcag aggttctgtt tcctggttca aaaaagcaac
     7021 agcgcccagt atgcgtggaa agaagggcgc tgtcactcag agtctcaacg agatccttct
     7081 cgctaaggtg ggttactgct gctgtaaacg tgcttcgatg gcatccatta acattccggt
     7141 gatcgacacc ggaaactctg cttcaatctc acgaatacag gttgggctgg tgacgttaat
     7201 ttcagtcaga cggtcgccga tgatatccag accaacaaaa atcagccctt tttctttcag
     7261 cgtcggcccg atctgacggg cgattttcca gtcactttcc gtcagcggac gaggttcacc
     7321 gcgaccaccg gcagccagat tgccacgggt ttcgcccccc tgcggaatac gcgccaggca
     7381 gtacggtacc ggctcgccat ccaccaccag cacgcgtttg tcgccatctt taatggctgg
     7441 caggtaattt tgtgccatgc agtagcgagt gccattctca gtcagggttt cggcaatcac
     7501 gccgaggttt ggatcgcctt ctttcacgcg gaaaatcgac gcgccgccca taccgtccag
     7561 cggcttaaga atgatgtcgc tgtgtttctc ccagaacgct tttagctgcg ctttattgcg
     7621 cgtaaccagc gtttctggcg ttaagtcaga gaaccaggcg gtaaacagtt tctcgttaca
     7681 gtcgcgcagg ctctgcggct tgttaacgat cagcgtccct ttctcttcgg cacgttccag
     7741 aatataggtc gcgtagataa actcggtatc aaacggcggg tctttacgca tcaggatcac
     7801 atcgagatcg gccagcggca gatcctgttc accgacgaac gaaaaccact cttcgtagtt
     7861 ctgcttcacg ttcagcgtgc gggtatgggc gcgggcttcg ccattgatca gatacagatc
     7921 gcccatctcc atatagtgaa gttcgtaacc acgacgctgt gcttccagca acatagcgaa
     7981 actggaatct ttcttgatgt tgatgtttgc gatggggtcc atcacgatgc cgagcttgat
     8041 cattattctt ctccgttagc ccaaatcgcc aaatcgtact tgtagcgcgg taatggcggt
     8101 gagcgcagtt gtctctgtac gcaaaacgcg aggtcccaac aggatatcag taaattgata
     8161 gcgggcagtc atggcaattt catctgccga taaaccgcct tccgggccaa tcagcaggcg
     8221 gacgcgttca accggtaacg gcaacgtatt gatgctgtta ctggcgcgcg ggtgaagatt
     8281 cagtttcagt ccttcatcct gctctgcaca ccaggcttcc agatccatcg ctggacggat
     8341 ttccggcacc cggttacgac cacactgctc acaggcagca attgcaatct tctgccactg
     8401 ctggagcttc ttgttcagac gttcactatc cagtttaacg ccgcagcgct cagaaaaaag
     8461 tggcgtaatg aggcttacac cgagttcgat cgatttctgg atcgtaaatt ccattttttc
     8521 accacgcgac atcacctgac cgaggtgaat atgcagcgga gattcgcgat cgtcgatctt
     8581 gccttccagc accttcactt ccacgctttt tttgctggcg ctggtaattt cggcgtcaaa
     8641 gacctggttg ctaccgtcaa acaattgcaa cgcctgcccc ggccccatgc gcagtacgcg
     8701 cccgatatgg ttggcggcat cttcgcaaag cgcgatgtga gaatggctgg tcagtggttc
     8761 aggatgataa atgcggggga tacgcatagt taaaaatccg cgtcgtctcc ccacgcggtt
     8821 gtacgcgtgg ggtaggggtt aacaaaaaga atcccgctag tgtaggttag ctctttcgcg
     8881 cctggcaagc gcgttgcaca tacgggttat gattgccttg caccttcgcg atgcgttcat
     8941 cgcgctcgca ctcccagtcg gtaaccggat acatcttgtt ccatgcgttg aacagctgcg
     9001 tttgctggcg agagagtgtc aggttgtatt ggtcgcgcat atagaagtag gtgcgcgcaa
     9061 tggcaccgcg tgcacgcgct ggtggttcgg cagctttttc tttgaaatcg accttcatgg
     9121 cgcattgacc gtactggcct tcgccgccat tccactggct gtacataaag ttgccgcgat
     9181 cgccattcac ctcaccgact gacggctgca ggttatacat atcgctttcc atcttgcgat
     9241 agaccggatc tttagcgcag tttttacgtc caccgtcctg ccagcactgg cgctggtgac
     9301 cgaactgcca ggcgggaacg acgtgttccc actctacgcg gctggctcgg ttttcatttt
     9361 tgcgcacctg atagccgcac gattgcagat caacaacgcc ttttttgccc tgccagttaa
     9421 ttttacatcc gcaataaaac gtaccgggcg cgtcagcgtg gacttttacc gccgcggctt
     9481 tcgcctgaga aaaactattg ataccttcgg ccaacgccgg gccggaaaat gctgcgctca
     9541 gtaccaccgc agcaatagac aaataacggt acatcactta ctccgtgtta aaacgagcca
     9601 gcaacgtagc gaaagttgtt ttggtatgca atcagccaga tcaggaaagt tcctgatagt
     9661 tcagatggtt atttcgcaac cagctgttca ccgcagtgaa cacagcgata gacggcctcg
     9721 ccacgaacta cgcgattatg gcggcgtacg gtaagctgat gctcctggca cttgcagcgg
     9781 taggggaagg tgttgcgacg cacggattgc agttcgaact gatgcgtacg acgggcggga
     9841 acacccagca cgctttccat catccacttc cactctttgc catgtggcgc tacgcggccg
     9901 aaatgtttcc ataccagcaa atgtgccagt tcgtgcggta ccacttcttc aataaaagct
     9961 tcactgtttt ccaacagcaa aacgggattg aggcggattt catagctttc cagccaggct
    10021 gttccggcgg aggttccgcg ctgggtgtaa gagagttttg gctccgggta gttacgccct
    10081 agcttcaggt tggcctgggc gagtttttcc cgcaggccgc gcataacggc ctgttggatg
    10141 gcgataggga gacgggatgt tttcatagcg gcagaggata gagcgaagaa aagatgactg
    10201 caagaggcgg cttcctccgc gacgggagga agcctgagga gattaatcgt gagcgccgat
    10261 ttcgcgcagt ttacgacctt tcatcaggtt acgttcaata tgttccagcg aaacgtgttt
    10321 ggtttccggt accagccaca atgtcagcag gataaacagt acgttcagag ccgcatacac
    10381 ccagaaggtg ttggcgttac ccagcgtgtt gagcatggtc aggaacgttg cgccaacgat
    10441 catgttggca atccagttgg tggcagtgga gcaggtgatg ccaaaatcgc ggcctttcag
    10501 cggctgaatt tcggagcaca gtacccaaat cagcggaccg gcactcatgg caaaaccgac
    10561 aataaacatc agcagcatgg cgatggcgaa atactgcgcc gacggagagt ggataccgat
    10621 atgcatcatt gtaccgagta cgcccatgcc agcagccatc accaggaagc ccagcgttag
    10681 cgttggtttt cgtccccagc ggtcaacaag gccgattgcg ataaaggtgg caagtacgtt
    10741 ggtcaggccg acaatcactg tcccccacat ttgctctgtg gtgttggtat aacccgccag
    10801 ttcgaagatt ttcggcgcgt aatacatgat gacgttcatc ccggtgaatt gctgcattac
    10861 ctgcaacagt acgccaagga acaccgcgcg gcggaagttg ctgttctctt taaacagcgc
    10921 ccagccactc tgtttaacct gcaaactttc acggatttca tccagttcgc gtttcgcttc
    10981 cgcgctggtg tcacgcaggc gtagcagcac gcgttcggca tcaacaaaac ggcgtttggc
    11041 ggcaaaccaa cgtgggctgt ctggcaggaa gaagacacca atcagcagca aaattgccgg
    11101 gatgataatc acacccagca tccagcgcca tgcaccggtg tagctgaagg cggtatcaga
    11161 aagataagca ccgaggatcc cgatagtgat catcaactga tacatcgaga tcatactgcc
    11221 acgaattttt tccggcgcaa tttcagagag gtacagcggt gcggtataag aggccacacc
    11281 caccgccagc cccagtagaa cgcgggaaag aatcagtact tcaacgtttg gcgcagccgc
    11341 agagaacagc gaaccggcaa caaacaaaat tgcgccgatc atcaggctct ttttgcgccc
    11401 gagtttaaag gagagccagc cgctgcccac cgcaccgact gccgcaccga acatcatgga
    11461 gcttacgacc cattcttgcg tgtgcgaagt aatctggaat tcatctgcaa taaacggcag
    11521 tgcgccagca attacaccga tatccaggcc aaagagtaat cccgccagag cggcaaggaa
    11581 gcagacgaaa aacgtcattg ccttgtttga ccgcccctgt tttttagcgt caggcatgat
    11641 gccctccaat atggttattt tttattgtga attaagatag gtgagtacga cgtaaaaaga
    11701 tgtgaagcaa atcacatcag tgtaatcgct tacactaaca tttatcttag ttatggttta
    11761 agtgcatgaa aataattgag ttatatcatc atagattaaa cgctgttatc tgcaattaag
    11821 actttactga aaagaaatgt aacaactgtg aaaactgaat aataatatga aaatattaca
    11881 aatatttggc tggtgttatt ggtgtaatcg gtttcagtag tgatgataat ttgcgataaa
    11941 acgtgttgag gaagaatgat gttattgtgc gggcagcttt ttgccggatg cggcgtgaat
    12001 gcctatccac cgcggtgagg taaaagaaaa aggccagcg
//
LOCUS       DAEEBB010000121        12037 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEEBB010000121 DAEEBB010000000
VERSION     DAEEBB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.121"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            9..323
                     /locus_tag="KLF07_003202"
     CDS             9..323
                     /locus_tag="KLF07_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD9833149.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="KLF07_003203"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="KLF07_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="KLF07_003204"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="KLF07_003204"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBD9833150.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="KLF07_003205"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="KLF07_003205"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBD9833151.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="KLF07_003206"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="KLF07_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBD9833152.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="KLF07_003207"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="KLF07_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBD9833153.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="KLF07_003208"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="KLF07_003208"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBD9833154.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="KLF07_003209"
     CDS             8424..8927
                     /locus_tag="KLF07_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBD9833155.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="KLF07_003210"
     CDS             9004..9687
                     /locus_tag="KLF07_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBD9833156.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="KLF07_003211"
     CDS             9766..10752
                     /locus_tag="KLF07_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD9833157.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="KLF07_003212"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="KLF07_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBD9833158.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAEEBB010000122        11877 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEEBB010000122 DAEEBB010000000
VERSION     DAEEBB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.122"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            486..1157
                     /locus_tag="KLF07_003213"
     CDS             486..1157
                     /locus_tag="KLF07_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBD9833159.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="KLF07_003214"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="KLF07_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="KLF07_003215"
     CDS             2363..2923
                     /locus_tag="KLF07_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBD9833160.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="KLF07_003216"
     CDS             2925..3698
                     /locus_tag="KLF07_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBD9833161.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="KLF07_003217"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="KLF07_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBD9833162.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="KLF07_003218"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="KLF07_003218"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBD9833163.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="KLF07_003219"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="KLF07_003219"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBD9833164.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="KLF07_003220"
     CDS             complement(6457..6996)
                     /locus_tag="KLF07_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBD9833165.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="KLF07_003221"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="KLF07_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBD9833166.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="KLF07_003222"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="KLF07_003222"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBD9833167.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="KLF07_003223"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="KLF07_003223"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBD9833168.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="KLF07_003224"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="KLF07_003224"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBD9833169.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="KLF07_003225"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="KLF07_003225"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBD9833170.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3177 c         2908 g         2880 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAEEBB010000123        11807 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEEBB010000123 DAEEBB010000000
VERSION     DAEEBB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.123"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..487
                     /locus_tag="KLF07_003226"
     CDS             <1..487
                     /locus_tag="KLF07_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD9833171.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="KLF07_003227"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="KLF07_003227"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBD9833172.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="KLF07_003228"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="KLF07_003228"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBD9833173.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="KLF07_003229"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="KLF07_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBD9833174.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="KLF07_003230"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="KLF07_003230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBD9833175.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="KLF07_003231"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="KLF07_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBD9833176.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="KLF07_003232"
     CDS             complement(6838..7746)
                     /locus_tag="KLF07_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9833177.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="KLF07_003233"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="KLF07_003233"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBD9833178.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="KLF07_003234"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="KLF07_003234"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBD9833179.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11807
                     /locus_tag="KLF07_003235"
     CDS             10714..>11807
                     /locus_tag="KLF07_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBD9833180.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3005 a         2999 c         2927 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacat
//
LOCUS       DAEEBB010000124        11777 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEEBB010000124 DAEEBB010000000
VERSION     DAEEBB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.124"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..609)
                     /locus_tag="KLF07_003236"
     CDS             complement(<1..609)
                     /locus_tag="KLF07_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBD9833181.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(732..2378)
                     /gene="fumB"
                     /locus_tag="KLF07_003237"
     CDS             complement(732..2378)
                     /gene="fumB"
                     /locus_tag="KLF07_003237"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD9833182.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="KLF07_003238"
     CDS             complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="KLF07_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBD9833183.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="KLF07_003239"
     CDS             complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="KLF07_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBD9833184.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5083..6714)
                     /gene="dcuS"
                     /locus_tag="KLF07_003240"
     CDS             complement(5083..6714)
                     /gene="dcuS"
                     /locus_tag="KLF07_003240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBD9833185.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6895..7125
                     /locus_tag="KLF07_003241"
     CDS             6895..7125
                     /locus_tag="KLF07_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBD9833186.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7137..7409
                     /locus_tag="KLF07_003242"
     CDS             7137..7409
                     /locus_tag="KLF07_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD9833187.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7636..7932
                     /gene="ghoS"
                     /locus_tag="KLF07_003243"
     CDS             7636..7932
                     /gene="ghoS"
                     /locus_tag="KLF07_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBD9833188.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7960..8133
                     /gene="ghoT"
                     /locus_tag="KLF07_003244"
     CDS             7960..8133
                     /gene="ghoT"
                     /locus_tag="KLF07_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBD9833189.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="KLF07_003245"
     CDS             complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="KLF07_003245"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBD9833190.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="KLF07_003246"
     CDS             complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="KLF07_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBD9833191.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11522..>11777)
                     /locus_tag="KLF07_003247"
     CDS             complement(11522..>11777)
                     /locus_tag="KLF07_003247"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBD9833192.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3042 a         2856 c         2892 g         2987 t
ORIGIN      
        1 gcgccgtagc tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag
       61 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
      121 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
      181 tatcgccagc gccacgcagc agactaggaa cgccaccatt ttacgtccgc gcacgtacat
      241 cccgcgcacg agaatttctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
      301 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
      361 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag
      421 cgtatagagc agcggcgtta acggataacg tttagcggtg gcaagcagta gcggcacgac
      481 aataatcacc ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt
      541 ataaatcagg attaacgtta gcgccagtgc gccggtgtta agaattaacg gtttgagtgt
      601 gcgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag caagcatgaa
      661 attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg
      721 ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgacgatttg ctggaagaag
      781 tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag
      841 atagcttcca tacccagctc cggataagcg acgcattcca gatgcttgat gctctgctgc
      901 gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg tttatgacac
      961 gcgtcggtaa cctgctggct gcggttgcct ttcgccagca tgatcatgct gccgccgtgg
     1021 gattgcagca gatccacata ggagtccata cgacctgcgg tggttgggcc aagtgaacct
     1081 gatggataac cggcaggagt tttcgccgga cccgcgtagt agatcgggtg atctttgatg
     1141 tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct
     1201 cgacccacaa taatggtgcc agtgagcgac aaacgagtgg aaaccggata ttgcgaaagc
     1261 tgggcgagga tctctttcat cgggcggtta aggtcgacct tcaccgcttc gccttcaccg
     1321 gcctgacgca gttcttgtgg aatgtactga cctggattgt gttccagttt ttcgatccag
     1381 ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga gacgcccatg
     1441 cctaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag
     1501 tatttaccgc caaactgcgc gccaagaccg agtttctggg cctcttccag cagttcctgt
     1561 tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg
     1621 taatagtgag cgcttgctaa tttgacggtt ttcaggttgg tttccgcaga cgtaccgcca
     1681 atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg cattttctcg
     1741 acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg gtagagatac
     1801 gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc atcgccatct
     1861 accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg
     1921 tccagcgccg cgttttgcga gtagcgcagg ttatcttcga tataggtgtt atagacgcct
     1981 ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt tttaccgacg
     2041 atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag
     2101 tttcttaaga attgcagtgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga
     2161 atagccgcaa cctgtttctg gtgtgccgga cggagcatga aagaagcgtc gtgaaaggct
     2221 tgctgcgcca gcagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag
     2281 tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc
     2341 gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt
     2401 atttgttatt cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata
     2461 agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca
     2521 accccggcag aataaagctg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac
     2581 ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc
     2641 aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa
     2701 caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa
     2761 tggcataggc ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca
     2821 tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga
     2881 tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga
     2941 taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg
     3001 gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagggcg actacagcga
     3061 ttgccccgag gaaaatccac attgccagcc agttgctttt cggcagtttt ttgtccagca
     3121 gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga
     3181 actcttcgtc tttatccaga tctttaccac ggaaccagct gaagataccg atcgccagga
     3241 taccgattaa cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac
     3301 catcaaaggt gacattaccc agcattgcaa ccagagacac gaccgcaacc gacaccggac
     3361 tggcgatgat ccccatctgt gcaccgatag aacttgccgc catcggacgt tccggacgga
     3421 tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg
     3481 taccgcaaag aatggtcagc gtacaggtca caaacggcgc gacaattgag acatatttcg
     3541 ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt
     3601 gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca accggtggtt
     3661 tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac
     3721 ccagcgcgat accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt
     3781 ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag
     3841 atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg
     3901 gtcgttaccg gctttagcaa atacctcaca gtgaatattg gctgattaaa gcagcggcaa
     3961 atttatgcac tgtgataagc ggctttttca gagagaaaga ctctctggcg atgggtttat
     4021 agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt
     4081 ctattttttg ttctcagggc agatgtcatt aggtttatag attaatctga tctacccatt
     4141 tgtgggtaaa aatacacata atgcgggtga cataatagtt aattaacttt tgttagcgtt
     4201 ttgaaattaa aaacaccgtt cacctgaaga gatattaatt tttagcgatg atgcagggat
     4261 aattatattt gatctggcac aagttttact gatgaaggat gtaacttgtg ccaggggtat
     4321 tttgcattac ggtaataatt attacttaca gataaccagc gatccgttat tggcaatatt
     4381 gtttcagtag tgagtagtgt tctgcctgaa tacggtaacg gtaaactgga cgccccgtga
     4441 cgccataatg gatactggtg aacaagatgt ggcagttgac cagccagatg aggtatttac
     4501 ggcaggaaac acgcgaaatg ttaacctcgt tggctagctc gtcggttgaa aattcatagt
     4561 cctgatgcgc gtcaatccac tggcacagtg tgcgtaacgt ctgcggcgtt aagccttttg
     4621 gcaagcgacg aggatcctgt tcgttggagc tgctgccgtg gattagctga tcaagctcgg
     4681 cctggtcata atactgatgt ttttccagcg ccattttctt ttgccgccag tcggtgagcg
     4741 cctcttcaaa gcgggaagcc tggaagggtt tgatcaggta atccacgaca ccgtaatgca
     4801 gcgaatcttt aatggttgcc gcatcggctg cggaggagat gacaatcaca tcacttttgc
     4861 aacgcgcgtt atgcaggaca ggcagtaaat cgagcccgtt ctctttttgc atatagatat
     4921 cgagcaatat caggtcgata ggcgtatcgc tattgaagat aatctctttg gctttctcca
     4981 gcgtcgaggc tgttccacag cattgaaagc ctgggatttg tgctacgtat cggcgattca
     5041 gctccgcgac cattgcgtcg tcatcgataa ttaatacatt gatcatctgt tcgacctctc
     5101 cccgtcccag ggtatctgga caaaaaattg tgtgaaaatc ccgggttccg attccacggc
     5161 gatgctgccg ccgagatttt ctacctgttg tttgacaagt gctaaaccga cgcctcgctc
     5221 gcttcctttt gtcgagacac ctttgtcaaa aatgtgatcg attttgtcgg gtgcgatccc
     5281 cggtccatca tcgttaactt cgcagtgcag ccagccgtga cggtagtgca atgttacgct
     5341 aatttcgcca ccgggttccg gccctaatgc ttccagcgcg ttttctatca gatttcccaa
     5401 tgtggtaatc agcgtcgcga cctggtcctc actgccgctg tctggcagct ggctttcact
     5461 gtttaaaatc agcgtatggc ctaaatcggt cgcgcgatta atcttgctga ttaaaaaacc
     5521 agcgataacc ggagatttga tcttacccag cagagagcca atctcttcct gatagttatt
     5581 ggctgttttt agaatgtaat cttccaactg cttataactc ttcagatgca ataatccgag
     5641 aatcacgtgc aatttattca taaattcgtg ggatcgttca cgaagtgcgt cggcatagtt
     5701 gaccagaccg tcgagtcgct gcatcagttt acgtacttcg gttttgtccc tgaaggttga
     5761 aatggcaccg atgataacgc cattactgcg caccggaacg gtgttgatca gtaatagccg
     5821 gtctttaatc gtaatctctt cgtcgcggcg cggcgtaccg tcgcgtaaca cttccgagac
     5881 atctaccacc tgtgaccatg agtggctgag cgtcgacagt ttctcatcgt cctgcgactt
     5941 acggtaattc agcaattctt gtgcggcatc gttgatcagc gtgacctcgc cgcgatcgtc
     6001 cacggcaacg acgccttctt tgatagactg caacatggcc tggcgttgct caaacagcgt
     6061 ggagatttcg tagggttcca ggccgaaaag gatttttttc agtaccttaa ccagaatgca
     6121 ggtgccaatc agtccgacca gcatgccaaa taataccgac cagataatgc tccagcgact
     6181 gtcattgatt tgttgggtca cgcggcttaa ctcaaggccg atcgccacca cgccaatttg
     6241 tttatgattt tcatcgtaga tgggggtaaa tacgcgtaaa gcctgcgcca gaaaaccgcg
     6301 attgatagcg acattttctt tgccattcag cgctttaagg atgtcatcac ctttaaatgg
     6361 ctgaccaata cgctgggctt caggatgcga gtagcgaaga ctttgcatat cggtaacgac
     6421 aataaacagc agatcgttgc gtttgcgcac ggcttccgcg atggcctgga tgccgctctc
     6481 ctgcggtttt ttctgcaagc cctgacggat ttccggcgag ttggcgaggg tacgcgccac
     6541 tgccagtgcc ttgttggcta gcccgtctcg cgtcatatca ctgatttgcg agaagtaaat
     6601 cagatgcacc accaatagca ccgagaacag taccgcactg accattaaga tcactgtggt
     6661 actcaatttc atcggacgtt tgcgtaacat gcggtagggc aatgaatgtc tcatcagctt
     6721 ccttgtgtga caaatttctt aaacattatc tctgatgagg tgggtaattc aaagggagta
     6781 agaatgattg gctatatagg ggaagagatt ctggcaacgg aaactgccag tgctgtatga
     6841 agattccagg gctatgctta tagcgataat catactgatg agagagggaa agtcatggat
     6901 caggcgctac tggacgaggg ttatcgctgt tataccggcg aaaagatcga tgtctatttc
     6961 aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa gttatttaat
     7021 ctcaaacgaa agccgtggat catgccggat gaagtcgacg tcgccactgt ggttaaagtg
     7081 attgatacgt gcccgagcgg cgcgctgaaa taccgtcata aataagcgag ggtaaaatgg
     7141 aaatacgcga aggccacaat aaattttaca ttaatgacga acaaggcaag caaatcgctg
     7201 aaattgtctt tgtgccgact ggagagaatt tagcgattat cgaacatacc gatgtcgatg
     7261 aaagcctgaa agggcagggg attggtaaac agctggttgc aaaagtcgtg gaaaaaatgc
     7321 gtcgggaaaa acgaaaaatt atcccactat gcccatttgc gaaacacgaa tttgataaaa
     7381 cgcgggagta tgatgatatt cgcagttgat gggagagtac agagtcacga tatttttcat
     7441 gctctccgcg gagtgatgca ggagagcatc tgaagggtag ggggatgcac aaagaatggg
     7501 cagagagcgc gtttttttgt cccaagtcat cccctttact gagcaaaaaa aagaatatct
     7561 cctatatgag aatcatcaat cggggttatc aagttttgcg tccccagagc attcaatatt
     7621 gataggagtc atattatgga aggtaaaaac aagttcaata cttatgttgt ttcttttgat
     7681 tatccatcat cttattcctc agtgttctta aggttaagat cattgatgta tgatatgaat
     7741 ttctcttcta tcgtggctga tgaatatggg ataccacgac aattgaatga aaattccttc
     7801 gcaataacga catcgttagc cgcaagtgaa atcgaagatt taatcaggct taaatgctta
     7861 gacttaccgg atattgattt tgacctcaac attatgacag ttgatgacta tttccgtcag
     7921 ttttacaagt agccaggaag gataagaaga acataaaaaa tggcactatt ctctaaaata
     7981 ttaatttttt atgtgattgg tgtgaacata tcctttgtca ttatctggtt tatctcacat
     8041 gagaaaacac atattcgttt acttagtgca ttcctggtcg gaataacctg gccaatgagt
     8101 ctgcctgtgg cattactttt ttctctcttt taggagctgt agttgagtgg acccgctggt
     8161 tatcgtgaaa cgaagcgtct ctcctcaaaa ctggatttgc ggaacgacga acgaagcagt
     8221 cgcctgactg cttcgttcat taaagtgaaa tttatttctg tgggcgcatc gccgggaaga
     8281 gaataacgtc gcgaatagta tgactgtttg taaacagcat aatcattcgg tcgataccaa
     8341 tacccagacc agcggttggc ggcagaccat attccagcgc agttacgtaa tcttcgtcat
     8401 agaacatggc ttcgtcgtca cctgcagctt tagcgttaac ctgttcctgg aaacgttcag
     8461 cctgatcttc tgcgtcgttt aattcgctaa aaccattacc gatttcacga ccaccgatga
     8521 agaattcaaa acggtcggtg atttccgggt taacatcatt acggcgcgcc agcggtgaca
     8581 cttctgccgg atattccgta ataaaggttg gctgaatcag atgtgcttct gccacttcat
     8641 caaagatctc cgtgataata cgtcccagcc cccagctttt ctctaccgta attccgatag
     8701 attcagctaa tgctttagca gcatcaaaat tatccaggtc ggccatatcg gtttctggac
     8761 gatatttttt gattgcttcg cgcatggtga gtttttcaaa cggtttgccg aaatcaaaca
     8821 catgctcgcc ataagtgact ttagtggtac ccagaacctc ttgtgccagg gtgcggaaca
     8881 gtgactctgt cagttcaatc aaatcgtggt aatccgcata cgccatgtag agttccatca
     8941 ttgtgaactc aggattatgg cgaacagaaa taccttcatt acggaagtta cggttgattt
     9001 cgaatacccg ttcaaaaccg cctacaacca gacgtttcag atacagctcc ggcgcgatac
     9061 gcagatacat atctaaatcc agagcattat gatgggtaat aaacgggcga gcagatgccc
     9121 cacctggaat tacctgcatc atcggggttt ctacttccat aaagccgcgc gcgaccatga
     9181 actgacggat agcggccaga atttttgaac ggacaacaaa cgtttgacgg gatttatcgt
     9241 tagcgatgag gtccagataa cgttgacgat aacggacttc ctgatcctgc agaccatgga
     9301 atttatctgg taaaggacgt agtgctttag tcagcaggcg caactcagta cagtgaatgg
     9361 aaagctcacc cgtttgcgtc ttaaacagcg taccgcgggc accgataatg tcacccagat
     9421 cccatttttt aaactgatcg ttataaacac cttctggcag gctatctctt gcaacgtaca
     9481 gttgaatacg gccaccgaca tcctgcaacg ttacaaagga ggctttcccc atgatacgac
     9541 gggtcatcat tcggccagca accgagactt caatgtttaa ggattccagt tcctggttat
     9601 tcttcgcatc aaactcttcg tgcaactggt cagaggtatg gtcgcggcga aaatcattgg
     9661 gaaacgccac accttgctga cgtagtgccg ccagtttttc gcggcgattt ctcagttcat
     9721 cgttaaaatc aatagcctca ttggctcccc gtgtttcttg ttcagacatt ttggttcctc
     9781 taaatccagc tttcaattta gcgtagataa agagacagat cggtacactg tattatctgc
     9841 ctcgactatt aataactcag tttatcttat cgaaactaac gcttacctca agttgatatc
     9901 actaataaat aaccatcaaa atggtttaaa tgacttatta atctcgattt actagtgtga
     9961 ttaactgaaa gttaatttat tcataaccaa ttgtttttac gacagttaat cgttgctctc
    10021 ctgtatcata ttcgtcggcg tgctaaacag aaaacgggta gcaaaggcca atacgacgat
    10081 gatcgcgaca caagccaacg tccactctcc catctgagaa aagaatcgct ggtaagctgc
    10141 aattaccata ccgctaattt gcgactctgt cgtctgctgt gccacgacgc ctgccagcca
    10201 gttggcgacc gcgcctgttg ccagcatata aataccggtt aatacgccag acattttcag
    10261 acgcgtgatt tgcgcaatcg ccaccgggtc aataaagagt tctgcaaagc ccattagcgc
    10321 caacccggat atcatcacgc ccattgacgc ttgaccgtca gccgctgcat gtcgggcatc
    10381 aaatgccaac aacataaagc cacaagccat cagcagtaag ccaaaggcaa acttcagcca
    10441 gacgcgcaat gttgagttgc cgcggctttc tggcgacgcc agccaggcca gtacaacccc
    10501 agcgagcatc actgcaattg cattcaccga ctggaatagt gctgtaggta cttcaatatt
    10561 gaatgcctga cgattcacaa agcgatcgat aaacaagctg atggtactgc cgccctgttg
    10621 tgccagtacc cagaacaacg tcccgacaaa catcaacaat acaatttgcc aaagagcacg
    10681 gcggtgttcg gggaatttga tcatcatgcg ggcaatgatt tgtgcggcaa tgaggcaaac
    10741 gatcgccagc aaatatccgg accagtcgtt ctccagcagc agggtaaaaa atactggggc
    10801 taaacagagc atcaccacta accagctcca tactggtaag gcaaatttga cgctagtgag
    10861 cgctttttta tccatactac gcgtggactg gaaatgacga tgaccgctta agaaaatcaa
    10921 caaaccgata aacatgccgc cacccgcaag ggcaaagcca acatgccatc cataccactg
    10981 agcagccagg ccgcaggcga ttggggcggc gatagaaccg atattgcccg cagcatacag
    11041 cagcgaaaaa ccgccatcac gtcgatgatc gttctcgtcg tagagctcgc caagcaaaca
    11101 gctgatgttt gatttgaata aaccgtagcc acaaataatg attgccagcg ccagatacag
    11161 gctaaaggtt gaatttgtat caatgcccag caccacatgg ccaagggtca ttaacagcgc
    11221 gccggcaatc actgcagtgc ggttgccgag caggcggtcg gcaagccagc cgccgagaat
    11281 aggggtaacg taaaccagag aagcatatgc gctgaacagg ctgatggcat ggttatcatc
    11341 aaaaccaagc tgatgggtga gatagagaat gagtaaggca cgcatgccgt aaaaactgaa
    11401 gtactcccag atttggatcg ccacgatata gtatatcgcg cgcggctgtg agggtgtttt
    11461 catgtgttct ccttatgagc aaaaaaggga agtggcaagc cacttccctt gtacgagcta
    11521 attatttttt gctttcttct ttcaatacct taacggtata gcggccatca gcctgacggt
    11581 atgcaccgtg aatatcggtt tcaaatcccg gatagtgagt gccgatttca cacagcatct
    11641 gcaggaactc cagaaccgga cggctttctt cggtgatcat ttcacccggc attaccagag
    11701 gaactcccgg cggatacgga aggatcatat tggcgttaat acgacctacc atttcgtcga
    11761 ggtaaacttc ttcggtc
//
LOCUS       DAEEBB010000125        11541 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEEBB010000125 DAEEBB010000000
VERSION     DAEEBB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.125"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            460..1386
                     /locus_tag="KLF07_003248"
     CDS             460..1386
                     /locus_tag="KLF07_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833193.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="KLF07_003249"
     CDS             complement(1336..2529)
                     /locus_tag="KLF07_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833194.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="KLF07_003250"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="KLF07_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBD9833195.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="KLF07_003251"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="KLF07_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBD9833196.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="KLF07_003252"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="KLF07_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBD9833197.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="KLF07_003253"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="KLF07_003253"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBD9833198.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="KLF07_003254"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="KLF07_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBD9833199.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="KLF07_003255"
     CDS             complement(11165..11308)
                     /locus_tag="KLF07_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833200.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2925 a         2896 c         2949 g         2771 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggactt cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag a
//
LOCUS       DAEEBB010000126        11428 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEEBB010000126 DAEEBB010000000
VERSION     DAEEBB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.126"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="KLF07_003256"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="KLF07_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBD9833201.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="KLF07_003257"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="KLF07_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBD9833202.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="KLF07_003258"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="KLF07_003258"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBD9833203.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="KLF07_003259"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="KLF07_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBD9833204.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="KLF07_003260"
     CDS             complement(3928..5214)
                     /locus_tag="KLF07_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD9833205.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="KLF07_003261"
     CDS             complement(5235..6026)
                     /locus_tag="KLF07_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBD9833206.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="KLF07_003262"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="KLF07_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBD9833207.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="KLF07_003263"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="KLF07_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBD9833208.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="KLF07_003264"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="KLF07_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBD9833209.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="KLF07_003265"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="KLF07_003265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBD9833210.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="KLF07_003266"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="KLF07_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBD9833211.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="KLF07_003267"
     CDS             complement(9882..10364)
                     /locus_tag="KLF07_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBD9833212.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="KLF07_003268"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="KLF07_003268"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBD9833213.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAEEBB010000127        11355 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEEBB010000127 DAEEBB010000000
VERSION     DAEEBB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.127"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="KLF07_003269"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="KLF07_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HBD9833214.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="KLF07_003270"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="KLF07_003270"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBD9833215.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="KLF07_003271"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="KLF07_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBD9833216.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="KLF07_003272"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="KLF07_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBD9833217.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="KLF07_003273"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="KLF07_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBD9833218.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="KLF07_003274"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="KLF07_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBD9833219.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="KLF07_003275"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="KLF07_003275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBD9833220.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="KLF07_003276"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="KLF07_003276"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBD9833221.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="KLF07_003277"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="KLF07_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBD9833222.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="KLF07_003278"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="KLF07_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBD9833223.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="KLF07_003279"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="KLF07_003279"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBD9833224.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="KLF07_003280"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="KLF07_003280"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBD9833225.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="KLF07_003281"
     CDS             10898..>11355
                     /locus_tag="KLF07_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBD9833226.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAEEBB010000128        11304 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEEBB010000128 DAEEBB010000000
VERSION     DAEEBB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.128"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..208
                     /locus_tag="KLF07_003282"
     CDS             <1..208
                     /locus_tag="KLF07_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9833227.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="KLF07_003283"
     CDS             300..2984
                     /locus_tag="KLF07_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBD9833228.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="KLF07_003284"
     CDS             2981..4069
                     /locus_tag="KLF07_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBD9833229.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="KLF07_003285"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="KLF07_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="KLF07_003286"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="KLF07_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="KLF07_003287"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="KLF07_003287"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBD9833230.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="KLF07_003288"
     CDS             8928..9479
                     /locus_tag="KLF07_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBD9833231.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="KLF07_003289"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="KLF07_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBD9833232.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2965 a         2772 c         2934 g         2633 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt ttaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
    11041 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    11101 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    11161 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    11221 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccgattaca
    11281 taattaatag ctcacctttg tctg
//
LOCUS       DAEEBB010000129        11297 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEEBB010000129 DAEEBB010000000
VERSION     DAEEBB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.129"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..595)
                     /locus_tag="KLF07_003290"
     CDS             complement(<1..595)
                     /locus_tag="KLF07_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833233.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1025..1348)
                     /locus_tag="KLF07_003291"
     CDS             complement(1025..1348)
                     /locus_tag="KLF07_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833234.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            complement(1516..1935)
                     /locus_tag="KLF07_003292"
     CDS             complement(1516..1935)
                     /locus_tag="KLF07_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833235.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            complement(1935..2225)
                     /locus_tag="KLF07_003293"
     CDS             complement(1935..2225)
                     /locus_tag="KLF07_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833236.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            complement(2546..2836)
                     /locus_tag="KLF07_003294"
     CDS             complement(2546..2836)
                     /locus_tag="KLF07_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833237.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            complement(2836..3725)
                     /locus_tag="KLF07_003295"
                     /pseudo
     CDS             complement(2836..3725)
                     /locus_tag="KLF07_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4089..4751)
                     /locus_tag="KLF07_003296"
     CDS             complement(4089..4751)
                     /locus_tag="KLF07_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBD9833238.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(5120..5671)
                     /locus_tag="KLF07_003297"
     CDS             complement(5120..5671)
                     /locus_tag="KLF07_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9833239.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            6258..6605
                     /locus_tag="KLF07_003298"
     CDS             6258..6605
                     /locus_tag="KLF07_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBD9833240.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            6658..7053
                     /locus_tag="KLF07_003299"
     CDS             6658..7053
                     /locus_tag="KLF07_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBD9833241.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            7094..7837
                     /gene="cmoA"
                     /locus_tag="KLF07_003300"
     CDS             7094..7837
                     /gene="cmoA"
                     /locus_tag="KLF07_003300"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBD9833242.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7834..8805
                     /gene="cmoB"
                     /locus_tag="KLF07_003301"
     CDS             7834..8805
                     /gene="cmoB"
                     /locus_tag="KLF07_003301"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBD9833243.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8970..>11297)
                     /gene="torZ"
                     /locus_tag="KLF07_003302"
     CDS             complement(8970..>11297)
                     /gene="torZ"
                     /locus_tag="KLF07_003302"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBD9833244.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         2909 a         2629 c         2363 g         3396 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag aaaaataaga gagaattgct ctcccataat taaaattatg
      901 ccatgcttaa ggttatttgt caacagcttt ttaacaggtt gataacatca attcttattt
      961 accgcattgt taataataca accaacaaat aagccaagca acgtccaagc aattattaaa
     1021 cccattagtg aaccctcaca gaatccgtca ccagaatatt atcactgtac aggattgtca
     1081 cagcaccttt caaaaattgc tctggggaaa tactgtatat gttcgtttgc tcgataacca
     1141 atcttttcat gttgctgtct gtaattggta gcatcattcg aggctcgtta aactccaccg
     1201 actgaataaa gtcagagatg ttttctacat tatcagccat cagctttagt aatttatcaa
     1261 aattaattgc gatacctgca tcaatgaagc tttgcaccat catagcagtg tcgttatcaa
     1321 ccataatttg cattgggtaa ttattcattt ttaaatctcc tatgaaacac acctcagacc
     1381 tcttaaaata gccattctga ggccagtaac catgcgggtt acagagggag ttgcactggc
     1441 tggtaaggta atttatcaaa ttaaggtgca actctgcctg taaagtgcat tctaccgttt
     1501 cttagatggt tatttttaat tgtctttatt aatcaatggc ttaatgtaga aatactgtct
     1561 ccgtgaaata ccatattttt ctgtaatgta tcgctgagat tgaccagcat ttatggcctg
     1621 agtgatatca tcacggttat agcggttacc acgggattta tatttgtcac catccacatc
     1681 ttctgaggta ttgaagtctt caatcagctt aattgcatcg tgaaggtcat cttctgaggc
     1741 ataatcgaag gtatggataa tctcaaaatc accagtatct gctggtgcat tcttccatct
     1801 ggagaagcgg gattttaatt gctggtgtgt gaagggttca gcattacgag ttaaatcttt
     1861 ccatgttccg ttattctcac ggacaaggtg acctctatgg cgataaaacg catgataatc
     1921 aaatgtgcat agcattaaac ccccagctta tccgccagtt gatgaacctt ggcactcaac
     1981 acctgaatca ttgagcaacc tgccactgcg gactgtgctt tgagtagttc cagttggccc
     2041 gttgtgattc tgatggcctt agaaccctct gcaacgccct tgatagcgtc aaacggtgta
     2101 agggctatgt gttccttcaa aatctcacca acaagtgctg tgtggctcat ggctgggtat
     2161 ttgtctttga ttgctgccag tctggaatat gcctctggca aaaggcgtgt aactgtgtgt
     2221 ctcattaaat tttccttctt aaaatctttg acttataact aactttaaca tatttataac
     2281 atattgtcaa caactcattt acatgttaat atggttgtag ggggctgata caggtcattc
     2341 cctttgataa ataaggggaa ttaatgggct ttcttgaaac aattgctgaa tctcacagat
     2401 tcgcaagctt aggttgcttt gtgtttctgg tgcttcttgt gtatcagctt ttaaaaatga
     2461 ttatcgccag aagtacacga ttcaatttca gcattaagct tttcaaatat gaaatcacaa
     2521 ttacgattga tgatggtaaa acaaactaac gttttgtgat aatattggtg gcctttaagt
     2581 tgctggggtc accgtctttt aatcgtagct ggtggattga attccagata tcaaaatgat
     2641 acagcactgc aaacctcaaa gcgtcaatct ttaaatctgt cacaccatcg taaaagaaat
     2701 aacccctccc ttctttcttt ggtctaatcc ggtaaacttc cccgtgagta tccttcataa
     2761 gatgaccttc acgagaaatg ctgtaacgct ttaacaggta atctttgtat ctcttttcac
     2821 cgtgatattc tttcattact taattcctcc accggcaaaa tacatcttcg gtttaatttt
     2881 ttgcttcttg tcatcctgag tttgtttctt tttcggtgaa agataataat catcttcact
     2941 gtcatcgaat gggtctaaac ccgatacttt gttcttttgt cttgcaagct cgaatctctt
     3001 tttcttctct gccctcttaa tggctttttc acgttcatcc tcttcatcat cgaaggggtc
     3061 caactcatca tcagtggcgg ggcactgtgt gtttttatgg tcactatcaa cactcacatc
     3121 agtgatttct ctgtcttgtt tagaagtctt tttagagctt acttcaaggt cagattcatt
     3181 catagagaga aaatcattta attcatccaa agaaagaaag tcatcttctt taatatcatc
     3241 attaagtcca gtctctttat tcttatctgt aatattatta tctaatgttt tatctaataa
     3301 cttatctaaa ctctaacacg ggtgacattt tgtcactacc cccatgacat tttgtcactg
     3361 gtagtggtgt cattttgtct tactgaggct gcaacttcct gtgatgtagg tcttacaagt
     3421 aattccggct tatcatccag tgtgaataca cgataagtgc tttgacgccc ctgattcttt
     3481 tgcacctgaa ttaaaccgtg tttctcaagt gtctcaatgg ctctgattgc tgttctcttt
     3541 gatacaccag ccatataagc gagatggtca atacttggtg aatatgattt gtatttctct
     3601 tcatagaagt cacagctatg gaccatgacc atataaatca atcttgctgc tgcgttaatt
     3661 tcttctgaaa gttgagagat tcgcacatca aatttataaa actgatttgg gttatattta
     3721 gacattcata aattacctca tacgtgaaaa ggccaaccgg ataaaaccga ttagcctgtg
     3781 tgattaaaat atctggggga ttgatggact gataaagaag tccatgcctg aaacagtacg
     3841 atgattaaaa cgctgaggta taccctgaat gagaggttaa tatacgccaa ccatctgaca
     3901 cctccccaag tgttaccact tcaagacatg aactttttta tcagcccata tagaaactat
     3961 aacagatgcg attataaatt gcaacaatta tttcacagaa tgattactga ttggtcattt
     4021 attgttcaca gggatacttc ttaacatctg aatcacctgt cttgtaaatg accatattta
     4081 tgagaatttt agctcaacct ttgagcaatc ctgtatgacg tgctcagagg cattctgaga
     4141 gcatctgcaa tgtacttaat gggttttcct tgcctgtata gttttatgaa gctgtcgagc
     4201 gtctcaggtg ggtaaatggt gggtcttcct cttagtccac cacggttaat gatggttaac
     4261 ggtagcaatg tatcagcaca gcaaaccatc tgtgtaattt catgcttacg tggttctttt
     4321 ttaaaaacaa cgactgcact tggaaacggt gacctaatag taagcctgtt atgccctgct
     4381 ggtggaaggt atttaactgc acgatgaaga atacggattt catgacagtg gttattgctg
     4441 taaatgtgcc taagccagta gctggtgttg gctctatgcg gtatcaggaa agctactaaa
     4501 tccgcttcag acgcttttaa gaggaacgac ggaatatcgc tgtacggcgg gttacagaag
     4561 accttttcac taacccatga ttgacggctt atatcatcgg tgaatcgtgg taacagcgtg
     4621 tcatgctcag ttgcagcacc atctagactg aaaataaatt cacggtgtaa gttcttaaat
     4681 aacaaaaatg gagttctcca gtaatcagaa cccccatgat gcttcccgat taaaatatgc
     4741 tcaattgaca taagaaataa aaatttttat tgcaatcact cttgacaagg tttactctct
     4801 tttaagagat gcttaacagg ttttctaaac ggatatcctt tctgggatat ctatctttta
     4861 tcttaaaagt ttttacagat actttaaaga tatctgtata tattctgtac agtaactttt
     4921 cagagtctga aaatttttat aaaaaatgac tagccctctt tgaatctcat cttccccgaa
     4981 tcttcgaaaa tatctgtcta gttgattcgg tagaatctac ctcatgatgt atacgtgttt
     5041 agtacccgtc acaccagcta tgtgaccccc ttggcaaaaa agccaaatat cagctacatc
     5101 catgtaacta ttcagtaact tatttagcaa gtctgtaata agttgctcta gagattccta
     5161 gctcttcctg aatctgcaac ctcgttttac cttgtgaaat aagcatctgt acagcctctg
     5221 aagaagcttt tacaggtctt cccatatgta ccccttcagc tttagcacgt tcaataccag
     5281 cctttctacg ctctgctatc aagcttcttt caagctgtgc caacgttgac ataatgccta
     5341 acattgcttc acccatagcg cttgataagt caaatccttc tctaaggctg ataactgtca
     5401 cacctttagc ctgtagcgct ttaaccgttg ataacacatc aattgtgtca cgtccaagac
     5461 ggtctacagc taccacaact acggtgtcac cttctcgtgc ataagctaac aaactgctga
     5521 aaccgtttct taaagaagct ttgacagaac cactgacagc ttcatcagcg aaccatttag
     5581 caatgttgta ccctgcctct tcaatttgca tttgctggtt agtgatggat tggtcatctg
     5641 tactcaccct gcaatatcca agtatcgcca taatgttccc cttagaagtc tcaaaagtta
     5701 gtctcataag cttagctgtc tcataatcca aaaacaacct tttgagacta cttgagggtg
     5761 cattctaccg ttacgttgaa agtgtctcaa aaggacttcc ttatgagact ggttaataga
     5821 gcgctatgaa gattcaggag ggtaactata accatcactt tctaactttc acgtaggaaa
     5881 tcgttaagtt tcttgatagt tacaccatgt gaccaactat ctatatatcg ttatatagcc
     5941 ctgtatatag cactatatgt agtgtctgga gagggacgac taacacgata cagtttaccc
     6001 tgaaaagaga cttcacagat tctttatata tttcaataag ttatgctaaa aagagactat
     6061 accgatactg catagaattt gtgtaggatt gcatagatac ttggaaaaac accgttaaat
     6121 tgcatagtca ttcacctgat attgtcctgt ttttacaggt agttgattcg gatgttctag
     6181 gaatcatcct catagttgat tcggttagtt gggcttgtac caacccctaa tcaacctcat
     6241 agtgtattta gaccttgatg attttagaca gccgccctgt tcatgcagca cgtccacaca
     6301 gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat gctgtactga
     6361 cggcgacaaa tccgctgatt cgttttatcg gtagtgatga tatgacgcaa aaccgggaat
     6421 tatttcaggt ctggttacag aaattagcgc agtggcatca gaccactacg ccttatcttt
     6481 ttttacatac gccagacatt gcccaggccc cggaactggt acataccctg tgggaagact
     6541 tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa tcttctcttt
     6601 tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg agtttgtatg
     6661 gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc
     6721 gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa
     6781 agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat tgtgctgatg
     6841 ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg catcgttttg
     6901 cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg ctggcgacgt
     6961 tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat
     7021 atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt tctttttccc
     7081 ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg ccagactggg
     7141 cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc agcgttccgt
     7201 tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct tcgttcaacc
     7261 tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg
     7321 caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg cgatgattga
     7381 acgctgcagt cgtcatattg acgcctataa agcccctacg ccagtagacg tcattgaagg
     7441 tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca
     7501 attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag gactgaaccc
     7561 gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact
     7621 gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac tggagatcag
     7681 ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa cccataaagc
     7741 acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct ttaactttgg
     7801 ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa cttttattct
     7861 ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac
     7921 tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacgcggt ggaatttctg
     7981 cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga aagcgaagag
     8041 ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct gatgccatgg
     8101 cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg ttccgactgg
     8161 aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat tctggatgtc
     8221 ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca cctcgcggtg
     8281 gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa actactgggt
     8341 aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc actgaaagcc
     8401 tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct ggagcatctc
     8461 tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac gctggttatt
     8521 gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc
     8581 tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg ttttgtagat
     8641 atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac tgaatggatg
     8701 gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac agttgaaggt
     8761 tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact
     8821 gccggatgcg gcgtgaatgc cttatccggc ctacaaaccc ttgctaattc aatctattgc
     8881 agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt gcataacctc
     8941 agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt atcaaatgcc
     9001 gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga gttaccggca
     9061 caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc actgccgttt
     9121 ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac cacgcctttt
     9181 ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc
     9241 accagatcgc cattcgcaat actaaatcga gcagcatctt cggtgtgaat agtgatcggt
     9301 tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg actgtgtaaa
     9361 cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt acccttccac
     9421 tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa tttttccagc
     9481 gttttggagt aaatctcaat tttgccactt ggcgtaccca gcgcattttt caccggatcg
     9541 gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg catttcgatc
     9601 agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg cgcacgggcc
     9661 cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt accttcggta
     9721 tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac atcaaaatcg
     9781 tttcgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg ctggttgctg
     9841 taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg taatacgata
     9901 tctgcgtgtt tagcagctgc ggtccagtag cattcagaaa cgacgatcat ctccggtttc
     9961 tgccatgctt taatcagacg attagtatcc tggtgatggg taaagttacc gccacccgcc
    10021 caccagatca ttttgatatt cggatacgtt tgctctttac cgttatgctg atatttgccg
    10081 ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccctcat tccgccatca
    10141 tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag cacgccgcca
    10201 acacgtgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc gccgcccggt
    10261 gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt ctgttcacca
    10321 tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc cgccatcagt
    10381 tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc ccaggccgcg
    10441 ctcttcggcg tattatcgct cttacctctc agatactctt caaactgcgg atacccggta
    10501 gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc
    10561 cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc attatcgcca
    10621 aagaagtcga tggtttcgga gcggatagga tcaatggcaa tcactggttt gccagatttt
    10681 ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc aatttttaag
    10741 gtattcagcg ggttcatccc ccacagcacc acaacctggc tgttttccag aatcagcggc
    10801 caggaggtct gctgttcata cacctctaca gaaccgacca catgcggcat gatcacctgt
    10861 gccgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag gttcatataa
    10921 cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc gtatgatccg
    10981 gcaaaaatag ccgatggtcc gttagctttg cgaatgcgat cgtgttgctc atgaatcagt
    11041 tttaatgctt gttcccagct cacctgtaca taggtatctt cgccacgacc tttcgtcggt
    11101 tgcagtggat tatcgagata gctttttctc accatcggat gctgaatacg cgtcgtggtg
    11161 tgtacctgat ccgccgccgt agactgtaag gaattcggta tggttttcgc cagcgcgcct
    11221 gttgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca acgttccgcg
    11281 gtgaggattt taccgtc
//
LOCUS       DAEEBB010000130        11242 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEEBB010000130 DAEEBB010000000
VERSION     DAEEBB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.130"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..308
                     /locus_tag="KLF07_003303"
     CDS             <1..308
                     /locus_tag="KLF07_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBD9833245.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="KLF07_003304"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="KLF07_003304"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBD9833246.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="KLF07_003305"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="KLF07_003305"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBD9833247.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="KLF07_003306"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="KLF07_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBD9833248.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="KLF07_003307"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="KLF07_003307"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBD9833249.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="KLF07_003308"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="KLF07_003308"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD9833250.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="KLF07_003309"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="KLF07_003309"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBD9833251.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="KLF07_003310"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="KLF07_003310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBD9833252.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /locus_tag="KLF07_003311"
     CDS             complement(8116..9762)
                     /locus_tag="KLF07_003311"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBD9833253.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /locus_tag="KLF07_003312"
     CDS             complement(9765..9815)
                     /locus_tag="KLF07_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833254.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="KLF07_003313"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="KLF07_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBD9833255.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="KLF07_003314"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="KLF07_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAEEBB010000131        11201 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEEBB010000131 DAEEBB010000000
VERSION     DAEEBB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.131"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1284
                     /gene="narU"
                     /locus_tag="KLF07_003315"
     CDS             <1..1284
                     /gene="narU"
                     /locus_tag="KLF07_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBD9833256.1"
                     /translation="KTITYLSDTGCLEIQGASLAVTVNLNKIGFNFTTDQLFLLTALP
                     SVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALL
                     CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFL
                     GVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLH
                     LWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISD
                     KFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQM
                     IAVIFRQITIYRVKMKGGSDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLN
                     MTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1366..5106
                     /gene="narZ"
                     /locus_tag="KLF07_003316"
     CDS             1366..5106
                     /gene="narZ"
                     /locus_tag="KLF07_003316"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBD9833257.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5103..6647
                     /gene="narH"
                     /locus_tag="KLF07_003317"
     CDS             5103..6647
                     /gene="narH"
                     /locus_tag="KLF07_003317"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD9833258.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6647..7342
                     /gene="narW"
                     /locus_tag="KLF07_003318"
     CDS             6647..7342
                     /gene="narW"
                     /locus_tag="KLF07_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBD9833259.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7339..8019
                     /gene="narI"
                     /locus_tag="KLF07_003319"
     CDS             7339..8019
                     /gene="narI"
                     /locus_tag="KLF07_003319"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBD9833260.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8098..8991
                     /locus_tag="KLF07_003320"
     CDS             8098..8991
                     /locus_tag="KLF07_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBD9833261.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9087..9932)
                     /gene="nhoA"
                     /locus_tag="KLF07_003321"
     CDS             complement(9087..9932)
                     /gene="nhoA"
                     /locus_tag="KLF07_003321"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBD9833262.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10105..10674
                     /locus_tag="KLF07_003322"
     CDS             10105..10674
                     /locus_tag="KLF07_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBD9833263.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10678..10905)
                     /gene="pptA"
                     /locus_tag="KLF07_003323"
     CDS             complement(10678..10905)
                     /gene="pptA"
                     /locus_tag="KLF07_003323"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBD9833264.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11005..>11201)
                     /locus_tag="KLF07_003324"
     CDS             complement(11005..>11201)
                     /locus_tag="KLF07_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833265.1"
                     /translation="VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMGRNYLASVLTGSGLS"
BASE COUNT         2605 a         2917 c         2991 g         2688 t
ORIGIN      
        1 aagactatca cttatttaag tgatactggt tgtctggaga ttcagggggc cagtctagca
       61 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
      121 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      181 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      241 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      301 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      361 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      421 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      481 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      541 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      601 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      661 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      721 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      781 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      841 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      901 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      961 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
     1021 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1081 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1141 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1201 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1261 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1321 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1381 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1441 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1501 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1561 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1621 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1681 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1741 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1801 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1861 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1921 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1981 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     2041 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2101 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2161 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2221 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2281 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2341 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2401 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2461 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2521 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2581 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2641 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2701 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2761 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2821 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2881 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2941 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     3001 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3061 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3121 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3181 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3241 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3301 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3361 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3421 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3481 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3541 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3601 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3661 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3721 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3781 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3841 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3901 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3961 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     4021 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4081 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4141 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4201 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4261 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4321 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4381 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4441 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4501 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4561 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4621 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4681 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4741 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4801 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4861 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4921 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4981 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     5041 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5101 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5161 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5221 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5281 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5341 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5401 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5461 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5521 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5581 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5641 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5701 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5761 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5821 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5881 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5941 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     6001 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6061 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6121 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6181 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6241 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6301 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6361 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6421 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6481 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6541 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6601 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6661 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6721 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6781 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6841 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6901 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6961 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     7021 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7081 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7141 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7201 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7261 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7321 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7381 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7441 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7501 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7561 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7621 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7681 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7741 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7801 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7861 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7921 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7981 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     8041 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8101 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8161 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8221 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8281 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8341 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8401 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8461 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8521 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8581 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8641 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8701 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8761 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8821 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8881 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8941 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     9001 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9061 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9121 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9181 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9241 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9301 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9361 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9421 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9481 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9541 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9601 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9661 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9721 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9781 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9841 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9901 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9961 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
    10021 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10081 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10141 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10201 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10261 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10321 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10381 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10441 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10501 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10561 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10621 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10681 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10741 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10801 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10861 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10921 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10981 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    11041 gtagtttctg cccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    11101 atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt
    11161 tcctcttctt attttgcagt cgtcttcatt cattaccacg t
//
LOCUS       DAEEBB010000132        11003 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEEBB010000132 DAEEBB010000000
VERSION     DAEEBB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.132"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..280)
                     /locus_tag="KLF07_003325"
     CDS             complement(<1..280)
                     /locus_tag="KLF07_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9833266.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(447..1187)
                     /locus_tag="KLF07_003326"
     CDS             complement(447..1187)
                     /locus_tag="KLF07_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBD9833267.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1280..1915)
                     /gene="argO"
                     /locus_tag="KLF07_003327"
     CDS             complement(1280..1915)
                     /gene="argO"
                     /locus_tag="KLF07_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBD9833268.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="KLF07_003328"
     CDS             complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="KLF07_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBD9833269.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="KLF07_003329"
     CDS             complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="KLF07_003329"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBD9833270.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="KLF07_003330"
     CDS             complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="KLF07_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBD9833271.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5779..6798)
                     /gene="epd"
                     /locus_tag="KLF07_003331"
     CDS             complement(5779..6798)
                     /gene="epd"
                     /locus_tag="KLF07_003331"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBD9833272.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7170..7601
                     /locus_tag="KLF07_003332"
     CDS             7170..7601
                     /locus_tag="KLF07_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD9833273.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7624..8202
                     /locus_tag="KLF07_003333"
     CDS             7624..8202
                     /locus_tag="KLF07_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBD9833274.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8203..8910
                     /locus_tag="KLF07_003334"
     CDS             8203..8910
                     /locus_tag="KLF07_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBD9833275.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8898..9575
                     /locus_tag="KLF07_003335"
     CDS             8898..9575
                     /locus_tag="KLF07_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBD9833276.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9569..10246
                     /locus_tag="KLF07_003336"
     CDS             9569..10246
                     /locus_tag="KLF07_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD9833277.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10218..10931)
                     /locus_tag="KLF07_003337"
     CDS             complement(10218..10931)
                     /locus_tag="KLF07_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBD9833278.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10928..>11003)
                     /locus_tag="KLF07_003338"
     CDS             complement(10928..>11003)
                     /locus_tag="KLF07_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9833279.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2920 c         2740 g         2716 t
ORIGIN      
        1 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
       61 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
      121 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
      181 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
      241 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
      301 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
      361 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
      421 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
      481 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
      541 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
      601 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
      661 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
      721 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
      781 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
      841 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
      901 tctggctggg tgcgtaagtt cgctgagctg atatcttttt tcgcgatctg attgagttca
      961 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
     1021 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
     1081 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
     1141 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
     1201 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
     1261 tttttccatc agatagtgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
     1321 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
     1381 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
     1441 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
     1501 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
     1561 tcactgccag catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
     1621 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
     1681 aggctacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
     1741 tgccaccaaa aatcccggcg caaatcagga ccaaatcgct gatagcacaa agtaaggcaa
     1801 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtggaccga
     1861 gcggcagaat catagctgcc ccaagagcaa taccttgaaa gtaataagaa aacacgttgg
     1921 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
     1981 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
     2041 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
     2101 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
     2161 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
     2221 acgatgcacc aagttcgttc agacgcacag tcatttcgcg atctttcaaa atgcgatctt
     2281 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
     2341 cgccaataat aaattcgtta cggcgaactg gctcgcgaga gaagttaata atatttccgg
     2401 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
     2461 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gagatcaaca tattctccgg
     2521 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
     2581 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
     2641 gtacacccac gcgtcccagg gcagcgatta gcgtaaaggc gataataccg taacggacta
     2701 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
     2761 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
     2821 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
     2881 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
     2941 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
     3001 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
     3061 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
     3121 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
     3181 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
     3241 gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg ttcagttcct
     3301 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
     3361 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac tgcccctgca
     3421 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
     3481 cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt tcctgagcag
     3541 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
     3601 tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
     3661 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
     3721 tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
     3781 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
     3841 tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca
     3901 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
     3961 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
     4021 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
     4081 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
     4141 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
     4201 cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
     4261 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
     4321 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
     4381 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     4441 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
     4501 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
     4561 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
     4621 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
     4681 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
     4741 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
     4801 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
     4861 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
     4921 gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
     4981 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
     5041 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
     5101 tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac tttagaacca
     5161 ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag cgcgtccagt
     5221 tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat accgtgagta
     5281 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
     5341 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
     5401 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccaga
     5461 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
     5521 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac tttcgcgcct
     5581 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
     5641 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
     5701 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
     5761 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
     5821 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
     5881 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
     5941 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
     6001 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
     6061 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
     6121 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
     6181 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
     6241 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
     6301 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
     6361 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
     6421 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
     6481 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
     6541 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
     6601 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
     6661 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
     6721 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
     6781 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
     6841 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
     6901 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
     6961 aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt ttcagccccg
     7021 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
     7081 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
     7141 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttccc
     7201 ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
     7261 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
     7321 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac ggtacattcg
     7381 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
     7441 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
     7501 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
     7561 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
     7621 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
     7681 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
     7741 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
     7801 ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt tgcacccgtc
     7861 gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg
     7921 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
     7981 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
     8041 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacagtgat aggcgcgaac
     8101 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
     8161 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
     8221 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
     8281 ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac gcggcggcag
     8341 gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
     8401 ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg gacgcatgct
     8461 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
     8521 gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
     8581 tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
     8641 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
     8701 gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct ggctgtccgt
     8761 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
     8821 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctaaca
     8881 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
     8941 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
     9001 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
     9061 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
     9121 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
     9181 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
     9241 ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
     9301 tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg gtcatcgcct
     9361 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt cgcctgacgc
     9421 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc
     9481 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
     9541 aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat cttatcgctg
     9601 gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
     9661 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
     9721 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
     9781 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
     9841 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
     9901 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
     9961 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    10021 agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg acgaaaattg
    10081 gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga gcgtggtagc
    10141 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    10201 tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    10261 gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc gtcggtacgg
    10321 tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg ccccaccaga
    10381 cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    10441 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    10501 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta ctgcggccac
    10561 gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac gtcaaatgtc
    10621 tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt gtaatggtga
    10681 aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg cacgagatat
    10741 tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    10801 gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    10861 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    10921 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    10981 tgaccattcc gctaaaacgc gcc
//
LOCUS       DAEEBB010000133        10929 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEEBB010000133 DAEEBB010000000
VERSION     DAEEBB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.133"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..312)
                     /locus_tag="KLF07_003339"
     CDS             complement(<1..312)
                     /locus_tag="KLF07_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBD9833280.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="KLF07_003340"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="KLF07_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBD9833281.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="KLF07_003341"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="KLF07_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBD9833282.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="KLF07_003342"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="KLF07_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBD9833283.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="KLF07_003343"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="KLF07_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBD9833284.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="KLF07_003344"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="KLF07_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBD9833285.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="KLF07_003345"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="KLF07_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBD9833286.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="KLF07_003346"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="KLF07_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBD9833287.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="KLF07_003347"
     CDS             complement(8292..9365)
                     /locus_tag="KLF07_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9833288.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10929)
                     /locus_tag="KLF07_003348"
     CDS             complement(9376..>10929)
                     /locus_tag="KLF07_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD9833289.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2830 c         2479 g         2769 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttac
//
LOCUS       DAEEBB010000134        10898 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEEBB010000134 DAEEBB010000000
VERSION     DAEEBB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.134"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..611
                     /locus_tag="KLF07_003349"
                     /pseudo
     CDS             1..611
                     /locus_tag="KLF07_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="KLF07_003350"
     CDS             608..1222
                     /locus_tag="KLF07_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBD9833290.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="KLF07_003351"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="KLF07_003351"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD9833291.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="KLF07_003352"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="KLF07_003352"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD9833292.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="KLF07_003353"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="KLF07_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD9833293.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="KLF07_003354"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="KLF07_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD9833294.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="KLF07_003355"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="KLF07_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD9833295.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="KLF07_003356"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="KLF07_003356"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBD9833296.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="KLF07_003357"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="KLF07_003357"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBD9833297.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="KLF07_003358"
     tRNA            9115..9190
                     /locus_tag="KLF07_003358"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="KLF07_003359"
     CDS             9796..9954
                     /locus_tag="KLF07_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833298.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2574 c         2778 g         2786 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagatactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat taaaatataa acagataatt atgtatctta
    10201 ttgatttata aggttgtttt taagtaaagg taaataaaaa ataaatagcc ataaatagct
    10261 ataaatacat cttaatacct atatcccttc ccttatggct agattgcgag aggtgcttaa
    10321 cgcgattttc agcgcccccc gatctggaaa atccagaatc cccaaatatc agcaaggaat
    10381 tttcttgcgt aatcaacatc gtttttacct attaaatcat tacacagaaa gcctttctgt
    10441 aatgtctctc actaaacttt cactaaacac gagaatgatc ggttttatgt cggatcagat
    10501 gctagaaaca gcaccacgtc tcacaagggc tgtaagcgat gaaaccagtg tttatgcggg
    10561 tgcaggtcaa aatataggcc aaaatccatt taacataata aatgttatat gtgctaagga
    10621 acacatataa cgccttgaat tgatgtatca gtggaaaagt gatggatttt ttacgtagga
    10681 gcacgaaagg gcttctattg acttttgtgt gctatgaaat ataattaaag ttttttgttt
    10741 ttattgtgtg atgtggtggg ttacacaggg gaatagatta aatggtgttc tgtttataca
    10801 ggtttatttg ttagatcaac aatttggcat ttctaaacag atgtttgaac gttgccaaat
    10861 gtcttgacaa cacaagaaaa gggcttgcta tgccaggc
//
LOCUS       DAEEBB010000135        10715 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEEBB010000135 DAEEBB010000000
VERSION     DAEEBB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.135"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="KLF07_003360"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="KLF07_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="KLF07_003361"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="KLF07_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBD9833299.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="KLF07_003362"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="KLF07_003362"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD9833300.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="KLF07_003363"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="KLF07_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="KLF07_003364"
     CDS             complement(4104..4388)
                     /locus_tag="KLF07_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833301.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="KLF07_003365"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="KLF07_003365"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBD9833302.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="KLF07_003366"
     CDS             complement(5289..6497)
                     /locus_tag="KLF07_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833303.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="KLF07_003367"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="KLF07_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBD9833304.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="KLF07_003368"
     CDS             complement(7292..8977)
                     /locus_tag="KLF07_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBD9833305.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="KLF07_003369"
     CDS             9247..9624
                     /locus_tag="KLF07_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBD9833306.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="KLF07_003370"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="KLF07_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBD9833307.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="KLF07_003371"
     CDS             10071..10358
                     /locus_tag="KLF07_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBD9833308.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..>10715
                     /gene="nfsA"
                     /locus_tag="KLF07_003372"
     CDS             10342..>10715
                     /gene="nfsA"
                     /locus_tag="KLF07_003372"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBD9833309.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2649 c         2713 g         2588 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttggg
//
LOCUS       DAEEBB010000136        10686 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEEBB010000136 DAEEBB010000000
VERSION     DAEEBB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.136"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..784)
                     /locus_tag="KLF07_003373"
     CDS             complement(<1..784)
                     /locus_tag="KLF07_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833310.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="KLF07_003374"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="KLF07_003374"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBD9833311.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="KLF07_003375"
     CDS             1860..2747
                     /locus_tag="KLF07_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBD9833312.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="KLF07_003376"
     CDS             2780..3790
                     /locus_tag="KLF07_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBD9833313.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="KLF07_003377"
     CDS             3860..5293
                     /locus_tag="KLF07_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833314.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="KLF07_003378"
     CDS             5392..6741
                     /locus_tag="KLF07_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBD9833315.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="KLF07_003379"
     CDS             6731..7618
                     /locus_tag="KLF07_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBD9833316.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="KLF07_003380"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="KLF07_003380"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBD9833317.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="KLF07_003381"
     CDS             8352..9224
                     /locus_tag="KLF07_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBD9833318.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="KLF07_003382"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="KLF07_003382"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBD9833319.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="KLF07_003383"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="KLF07_003383"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBD9833320.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2532 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAEEBB010000137        10667 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEEBB010000137 DAEEBB010000000
VERSION     DAEEBB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.137"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1619
                     /locus_tag="KLF07_003384"
     CDS             <1..1619
                     /locus_tag="KLF07_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD9833321.1"
                     /translation="KKTDSQAHENSDPTPSAETTTTPPIPDSIVYAPERSDAPISLNM
                     DEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMVNGASNDDRSVLAALAVATKFY
                     GGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAELEKLRKEMAVSKDAVVTHQPT
                     PELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTVSTAPASSPAEPGKTADAAPGE
                     EPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELKNRSGSKLYWGEQLGSLVKNHQ
                     KGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNGISVSHDNPDKGQRIQAIPVET
                     FMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHPVSAPQNANQVTEITSAAPDKL
                     TPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLYPALATPSADNSQLVINALTDK
                     GLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPLFAAVYTPEKQPDALYRNLGFE
                     QSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            1637..1984
                     /locus_tag="KLF07_003385"
     CDS             1637..1984
                     /locus_tag="KLF07_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBD9833322.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            1981..2673
                     /locus_tag="KLF07_003386"
     CDS             1981..2673
                     /locus_tag="KLF07_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBD9833323.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            2684..3667
                     /gene="traN"
                     /locus_tag="KLF07_003387"
     CDS             2684..3667
                     /gene="traN"
                     /locus_tag="KLF07_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBD9833324.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNGNMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            3670..4959
                     /gene="traO"
                     /locus_tag="KLF07_003388"
     CDS             3670..4959
                     /gene="traO"
                     /locus_tag="KLF07_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBD9833325.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQKKAVMQRRYDEQPETP"
     gene            4959..5663
                     /gene="traP"
                     /locus_tag="KLF07_003389"
     CDS             4959..5663
                     /gene="traP"
                     /locus_tag="KLF07_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBD9833326.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRALSETGMPPFNDADAGVQTWQTTQESSPSVQSTEAMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            5663..6190
                     /gene="traQ"
                     /locus_tag="KLF07_003390"
     CDS             5663..6190
                     /gene="traQ"
                     /locus_tag="KLF07_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBD9833327.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            6241..6645
                     /gene="traR"
                     /locus_tag="KLF07_003391"
     CDS             6241..6645
                     /gene="traR"
                     /locus_tag="KLF07_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBD9833328.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISSISIG"
     gene            6709..6897
                     /gene="traS"
                     /locus_tag="KLF07_003392"
     CDS             6709..6897
                     /gene="traS"
                     /locus_tag="KLF07_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBD9833329.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            6881..7681
                     /gene="traT"
                     /locus_tag="KLF07_003393"
     CDS             6881..7681
                     /gene="traT"
                     /locus_tag="KLF07_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBD9833330.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            7771..>10667
                     /gene="traU"
                     /locus_tag="KLF07_003394"
     CDS             7771..>10667
                     /gene="traU"
                     /locus_tag="KLF07_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBD9833331.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYISVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWALSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICARNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYIRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGS"
BASE COUNT         2824 a         2783 c         2716 g         2344 t
ORIGIN      
        1 gtaaaaaaac ggattcacag gcgcatgaga acagtgatcc cacgccctca gcggaaacca
       61 cgacgactcc accgatacct gacagtattg tctacgcccc ggaacgttct gatgcaccga
      121 tatcgctcaa catggacgaa attcttaaag agcttgactg ggaaaaacga gcagacagaa
      181 ccgtcctgta caaactggat ggcaaacctg ccttcattga ccgggtaaac cgactggaaa
      241 tggtcaacgg tgccagcaat gacgatcgca gcgttctggc cgcacttgct gtggccacca
      301 aattttatgg gggcgtcata gagctgacgg gcagtgatgc ctttaagcaa aaagcaatgc
      361 agttgattat cgagcacaac atcgatgtcc ggatgaaatt gccagatcaa cgggctgaac
      421 tagaaaaatt acgcaaggaa atggctgtca gtaaggatgc tgttgtcacc catcagccaa
      481 caccggaact taaccggaat atccctgaac aaccggctgt ccctgacccc gttcaggaaa
      541 aagaagccac acaatccccg gcgtctcctg ctgcccctga agcatcaacc gtcagcaccg
      601 ctcctgcatc atcaccggct gaaccaggca aaacggcaga tgcagcacca ggtgaagaac
      661 caccaggaaa acttcgcccg ggagagagcg tgactgccgt actgcacaat tttggtcgtg
      721 ctgaatatgc acccggcaaa ggagagagtt tctttgttga gcttaaaaac cgcagtggca
      781 gcaaactcta ctggggggaa caactgggaa gtctggtcaa aaaccatcag aaaggtgacg
      841 tggtgacact gacacttcag aaccgcgaac aattcatcct cccgggagag caaaaagcac
      901 gtttccggaa taagtggtcc atggagtcag ttaccaacgg gatctcggta tcacatgata
      961 atccggataa aggtcagcgt attcaggcga ttccggtcga aacctttatg aaagtggctg
     1021 cgcaaatcag ccaggggtgg cccgaagaga tgaaagcgtt acgtatgcca gaaaacgtcg
     1081 gctcacatct gtttatcggt gaagaccgac atccggtctc cgcgccacag aacgcaaatc
     1141 aggtaacaga aataaccagc gcagcccctg acaagctgac acctgttctg ggatcagtgg
     1201 ataaggatac gcgtgaactc aacctgctac ttgtccagag tgccgatgaa catctgcagg
     1261 gtgtggtccg gctgaacggc acattgtatc cggcactggc caccccatca gccgacaaca
     1321 gtcagctggt tatcaatgcg ctgaccgaca aaggtttacg ctttgcaggt tatggtgaag
     1381 cggtaaacca cgatgctgac agcacgaatc gccccgcacc ggagcttatg cagtttcatc
     1441 tgaaaacccg ggaagaaccg ctttttgctg ctgtctacac gccggaaaaa caacctgatg
     1501 cgttgtaccg gaacctgggc ttcgagcagt catggcagca gtggtccaac agccaaaagc
     1561 cggaggacag gcaggaaaaa actctgcatc aggatctctc ccacagcccc ggccgatgag
     1621 ccggtaagga agccatatga acatgcataa gtctctggtt gccgtgctcg gccttatcct
     1681 gatttcatcc acggcaagtg ctgcaacctg tcgtgcgggt agcgcggcac aggccggtag
     1741 taatacagga tatgagcgag cacgtgcagc tgcggatgca tggtcacaac gtgaaaacga
     1801 tgtttcctca tccctgcagt cctgtctgtc acgaatcaaa aaaatcagca taaacctccc
     1861 ccagttcccc agccttgacg acattctgtc gcagctggaa aaccaggtct gcgacgcggt
     1921 cgtggacaag gtgaatgaaa agctgccggg gaatattgat ccctggaagg actacaacct
     1981 atgaccgaaa ataccgagcc atcctcgcca tcacatccgg cacctgaccc ggaaatactg
     2041 aagagcacaa tcagggctat gaagcagtca gaacagcggg caaataatgt ccctgcgctg
     2101 ataaaagcgc ttttatgtac gggcacatgt cttcttatca gtattactgg taacgccatt
     2161 cagtactggc actcaacgaa tgtggagcgc gaatatttcg ctacagacaa tggtcgtctg
     2221 gtcaggctgg caccaacaag tcagccagca tggagtcaga atgatgcaat ggcctttggc
     2281 agccaggcac tggcgaccgc attcaacctg gattttgtgc actatcgttc acagatctcc
     2341 tccctgtctc cgcgtttttc tgacgaaggt tttgtcggat atgtgaatgc attacaggca
     2401 tctaacattc tggagacgat caagaaagag aagatgaatc tgaccgccac gacaggtgct
     2461 ggcgttctgg tacgacaggg acaaatgtcc gacggcgtat ggttctggac attccagtat
     2521 cctgtccgta tgcgtctggt cggccagaca accagcaagc cagaacagtc ttttgttttc
     2581 gaaatcacca tccagcgtgt tgaccccaga ctgaaaccat ccggtatgga aatccggcag
     2641 atgatctccc gtaacgccgg accgaactcc tgagataagt aatatgcaat cacgttattt
     2701 actgagcaca ctgctcctgg tgtgcagcgc cgccacgtct gccgataacg caggatggca
     2761 gaacgcacgc acaccacaaa cgacaaattc tgccagcgat cacgttcaga atgcctccca
     2821 aaacaccgga ggtgttccgg caacactggt aaaaggtgag ctgcctgccc ccgggcaggc
     2881 cagcccgctg gtacaggatg cggcaagact tgattctgag ctctccgccg atgaaattcg
     2941 ctcactacgc agtctcatgg cggataacga gcgggccatc aatgcgccca tcaccagtgt
     3001 tgtaccccgt atcagctctc tgacagttaa tctgtccccc ggtgccagcc ttcctcttgt
     3061 tcgcacagcc atgaacaacc tgagcgtggt gacatttacc gatatcaacg gttcaccatg
     3121 gccacaatcc gatcctcctt ataacgcggc accaaaactc tttgatgtgc agtacaacgg
     3181 gaacatggtg acaatcacac cattgcggcc atgggcatcc ggaaacattt ctgtctacct
     3241 gaaaggtctg tcggtaccgg tcatcctgaa cgtaaccagt ggcgaaacag atacgccgtc
     3301 atccagtcag gaaatggaca gtcgtctgga tctgcgaata ccacggcagg gaccaaccag
     3361 cccggttgtc tccatcccga cggataaaat cgccctgcat gatgcaacat tacaggcatt
     3421 tcttgatggt attcctcccc gggacccttc agttaagagg ctgaaattca caggtaatgt
     3481 cccggataca acgatctggc aacacggcga tgaccttctg gttcgttccc gggcgatgtt
     3541 gcgcgatgaa tttgaacaga ccttgtcctc cgccgacgga actcatctct ggaagcttcc
     3601 cgtcacccca ctactgactt tctctgtgaa tggccagtcc gttcacgtga caccagaact
     3661 ggagtaatca tgagtgctga acaggatgcc ggtaaatccg gaaagaaact ggctgccctg
     3721 cttggactgg gcagcatcat cttatttggc ggcggctata tcgcgttttc caaactatca
     3781 gggagtaaca gcgatatgca gtcagccgtg aacattaaca gtgcagcaag cggcggaacg
     3841 cgcagcgtta ccgaaacgcc tcattatcgt gaactgttaa gagcggataa tgaacgaggg
     3901 gccgcagctg cagcccgcaa taatcagacg ttcattgcca gtcttccaca ggggcttgat
     3961 attccggaca ctccggaaaa gcaacaacag cctgcagcaa aaccggaaaa ctatgctcat
     4021 cgccaggctt ccggcacacc acaggaggat cgagcagcca gtgaaaagcg tatggagcgg
     4081 ctgcaaaagc tgattgtaag aataaaagat cagcatcctg ccggtagtac accaaccata
     4141 gcaacgacta tgtggaataa atctccggca gaaacaacag ggcagaatgg tacgcagcaa
     4201 ttcgcattac agaatgccag cctgtccacg cctgtagcag aaaaagggat acaactgata
     4261 cctgctctga cccgtatacc ggcatatatt gatactgcag tggattctga taacccgtca
     4321 tccaaggtta tcgcaaccat tcccgctggc ccctgggcag gggcaacgct gttcagtccg
     4381 ggagtcaaac tggtgggtaa cggagtggag atccatttcg accggatgag ctggaacggc
     4441 atggacctta aggtcaatgc atatgcacaa cgggaagaca acctgatgtc atccgtggcc
     4501 agcaatgtga atacacgctg gtttaaacat atcattctgc cgtcagtgtt aggaggtgtg
     4561 ggcagtattg gcacacttta taaagatgcc aacacgcagg ttattcaggg taactatggc
     4621 acggttaccg gacgcgtggg aatgcccagt ggtgaagctg ttgcgggcgt tattgccggt
     4681 ggtatggcgg aaagaggaag tcagatatta acccgccagg cagaaagtga gccctataaa
     4741 caggtagaag tgtatcagca tgaagtcgta tcaattctgt ttgttgaccc ggtaatgaca
     4801 aacgacgcca ggtcatcttc cctttccagt ggcatttccc cttcagttaa tcgtacatca
     4861 caggcggaac aacgctcgca ggcacgaatg cagacagcca tggagcaaaa aaaagctgtg
     4921 atgcaacgtc ggtatgatga acaaccggag acaccataat gaaacctgaa acagaaatag
     4981 atgaaaccgg cagcttcggt gaacccgaag agaaaccagc ccccttctgg aaacgttccg
     5041 tctgggggat atctgtagca acatgggggc tatgtgcagt cgtgttgctc gcagccatat
     5101 ggtatctgtt cctgagagcg ctatcggaaa caggcatgcc tccgtttaat gatgcggatg
     5161 ctggcgtaca gacctggcaa accacacaag aatcatcacc ttccgttcag tccactgagg
     5221 ctatgactgt ccggacagga gaagatatga gccagcttgc ccgggatgta aaaacagagc
     5281 tggataaccg cgatgaaaaa attcaggcaa cactgaatat gcttcacgac agcattaaca
     5341 agctgggcga agccataaaa aaggatgaag aatacgcaca ggaaacgcgc cgtcagctgg
     5401 atgatatccg gagccgcctg aacggaataa tgacacagaa aagcgtcact gaatcctctt
     5461 ccacaccgca tccagcaaag aaaaaaacgt cgtctgtact taacggaatg aaaatcatgt
     5521 cgatggaaac tggtatggcc tggattcgct ggcagggtag tacctgggct gtcagagaag
     5581 gtcagacgct gggcaatgtg gtgatccagc ggattgatcc gactacccgg accatcatca
     5641 ccagtgcagg gacgttacgc tgatgaacat ggatgccctg accgccattg agaactttgc
     5701 cagcagtatt ttcagtgccg gaatggattt cctgtttacc tggggagaat tcatcggggt
     5761 gatcagcatg attaccctgt ttgcccgcgc ccgttcagcc ggtccggtaa aaatgtctcc
     5821 cggaaaattt attgcgggga tgttaaccag ctgcatgctc gtatcactcc cggcaatgat
     5881 taacgccgga ggcgtacaaa tgggattcag ggctgactca tttggcccga ttgcatatgt
     5941 gcaacctcag acgtttggcg ctgctgctgg cgcagcaaat gcagttctgt cgctggccaa
     6001 gctggccggt gtgggatttg tcatgaacgg gatatccatc tggcgcaagg ccggactcga
     6061 tggacatacc gcactgagtg ccagtgaatc cgtcagtaag ggaaacgtca aatttattgc
     6121 cggcgtcctg ctggtcttta tcgatcgtgt cctcaatgcc ctgctggcaa gcattggaat
     6181 cgtcttctga tttttttcac cttcatataa accactgata tatccattta aggagaaact
     6241 atgtcacgta ttcgtgtcgt agcggcccgt ctgtacgccg catttatcca cgcacaactg
     6301 tttgtaatgt catgccggag caaactgact aagttcctgc tgctcattcc catgatgttg
     6361 ctgcccaaag ccgttcttgc cgatggagat cttgccgata tggttcgcaa tgtcgaacaa
     6421 ggtgccaaaa cagcacagtc atcatcactg actattgcgc agttcatcgg cgtaattgcg
     6481 tttctcgggg gcctgattgg gctgaaaaaa gttgggaaac aaggggggat gggactggct
     6541 ggttgcattc tatctatcgt cattggggcg ctacttgccg ccggacctga aatgatgagt
     6601 cgcagccaga aacagttggg gatttcctca atttccattg gctaatcctg tccagggcac
     6661 tggcatttat ccggtgccct tcctgttcgg caatacaagg aggcacatat gtctgcctgg
     6721 agcccggagc acataacgtt acgacgaaca gaaaacggca atgtgattgc ccacgacagt
     6781 cgggacgatc aggaattcat ttttgcagaa tgctgttccg atgaaatcgc aaacatcatt
     6841 ctggaagccg tgaaacgtta caccagtacg gagcctgctc atgcaacaca gcattaaaga
     6901 cttgtggttg tatccctttc cagaaattga tgtggttcat acacaggagc cactgttacc
     6961 ggaaccggaa ttaacaacgc ccggtcgatg tatctgctgc cggcaaaacg tcaggcaccg
     7021 tttcagactg gatgactcat ggccactcag acagcttacc gacacaatca gtgacacacg
     7081 agttcgtctt aataaggcca cagaacatct ggataagtta aaaaagcgtg gtgaaccagt
     7141 cgcaaccggc gaaaaggaaa agtacaacac ggcagtaaaa gccgctgaac gggcgcttga
     7201 gcaggcccgt ctgtctgcca gacgtctttc actccgccat gtacagaaag ctgaaatcac
     7261 cagtacagag tcgttgtcag agaaagagca ggaactgttt catgaagacg gtccgcctta
     7321 cagcttatgt gctttctgcc acgcctggca ttcactgaat ggttatgccg cagcacaggg
     7381 cgttatggtg tggctacccg accttcatcc atccacggtc gtggccctta accgtcgttc
     7441 attacaggaa gttttcagta atgataagtt ccgggtccgg cggggacggg aagcgctctc
     7501 tgcactgatg caaaacagac tggcggttga ggataaattc cgcagtttcc gcccggccga
     7561 tttcgctgac gtttttcgtc gctatccacc ttccggtcgt tcgcccctgc gtgaaaaaat
     7621 gaacgggatt gccctgattc tgacaccaga ttcattcatc aaaaaagaat acgttgattg
     7681 atttttcttc atgccactcc agcgcggggt ggcattaaaa aaatcaaact gcacaaacct
     7741 ccctcctgta agtccggagt tccctcacct atgaacataa accgtttaat cttcactatc
     7801 gaagactgtc tgaacacgct atcccgtttc tctgtcgcaa gcagttttgt tgagtattgc
     7861 gatttacgta ccgtaattgg tctggacaga caggatcgtg aacgtcgccc atggctgaat
     7921 tcgccctata ttgcagccac aaagcgtgga gaatatatct ctgtctttga agtcagcggt
     7981 gctttcaggg aaatggatga agcatccgac cagactggcc caggttcact ggaatcgctg
     8041 atcaccagca tgagtgacag cctgaatacg gcatataaaa acagcggtca taaaatcagc
     8101 ttcgtttttg agcgtgatcc tgacatgggg aaagaagaaa tcgaagacat ggtcgcccca
     8161 caaaagcgct cactggctaa tacaggtatc cagttacagg atgtggtcga tgagaaagtc
     8221 acaacactgt ccccatggct ggtccgggag cgttgctggc tggcgatctg gagtggaccc
     8281 gaccttatca gcaacagcga tcgcaccgcc catgatgagc ttgtcagaag actggctgaa
     8341 cgggtaccga aagcacgctt tgcccagtct ccctggcagt gggccttatc tgcactgaaa
     8401 atccgccacg aagcttttct tgataatgtg gaacaggcac tgagacattc atctgatggc
     8461 ctgattctcc ggctactgga tatacacgag gtcgggcgtg aaatacgtcg tcagacagaa
     8521 cggcacagca caccgcgtaa ctggcaaccc cacctgcctg aagatgccca gccagcggga
     8581 tatcgctgga ctgacgatga atccgtacta catgctcctt cattgcattt gcagttattc
     8641 aacactcagg tgaccacaca gggcaacctg gttcaggcgg gcggactctg gcacggaatg
     8701 gtatcgatta ctttacctcc gcaaaatctg cagacgttta acgaactggt ccgtgccgtc
     8761 ccccgcgcag tcccctggcg catcagaatg gatctgatgc ccggcgggat gaaggctctt
     8821 aatctgaaga aaacgcttct gacttacagc agcttcattt ctgccgttcg tccaatgtat
     8881 gaatccgtta tgaccctggc ggcaacggat gaaaaagaac ctgtctgcat aatgaccatc
     8941 atggcctcca cgtggggaaa aacacgtgaa atatgcgcac gaaaccaggc cattctgaaa
     9001 tcggccattg aaggctgggg cgtatgtggc accacgacaa catttggcga tccccgacgc
     9061 gcctgggtga ataccattct cgcggcatcc ggaggctccg gcccggttcc cctgtatccc
     9121 ccgttgtcac atgccatctc gcttttcccg cttaacaggg caggctctgt ctggcgaggc
     9181 aaagggaatc tgatgttaca tacagaagac ggttccgcat ttgaggttgg tctggcgtca
     9241 tcccaacaaa ataaacatac agaactggca ccaggcgatc cgggactggg taaatcagtt
     9301 ctgattaata cgttgtcgga aatacagata tcatctgctc agaaaaacct gcccttcatt
     9361 gcctatatcg ataagggata cagtgctcag ggactggttc agcttattcg cgacagtctt
     9421 cctccggaaa gaaaagacga agctgtcggt attattctga gcaatgatcc ggaatacatc
     9481 cgtaaccttt ttgacgtgat gtacggcgct aaaaagccta ttacgccgga aaagaatttc
     9541 atgtcatctg tgctgtgtgc tctctgtgtc gataccggta ccggtcaacc ctgtaacccc
     9601 ggagacaccc gacaaattat caaccagctt atcgagctgg cttttaaaga gtacggtgaa
     9661 aataacccgc gactttatcg cgcttcaacg gaagaactgg tggattcagc actgcaggac
     9721 tcgggactgt atgaaaaaca tgatgcagcc tggtgggcca gatcaacctg gtttgaagtc
     9781 cgggatatgc tccacaatgc cgggtatatc atggcggctc agcgggctca ttatcaggca
     9841 atgccccagc ttcccgaagt gtcctcgatg ctgggccata ccagtctcag ggatgttttc
     9901 ggtactgttc agcgtgatgg ttcaaacgag cttctgctgg actacatccg ccgcgccctg
     9961 gagcagggac ataacgacta ccccatgata tccgggtata cccggtttat gatcaacccg
    10021 gaaacccggg tcatcgctgt cgatctgaat aatgttgccg gtgacaaaac gcctgcaggc
    10081 aggcttaaaa ccggcatcat gtacctgctt gcaggtcaga tcgccggtgg tgacttcacc
    10141 ctgccacaat acagggatga agtgttaaag cagcttccac gggaatacca tgaaatcgct
    10201 cttaaacgta ttaaccaact tgatcaggag gttaagacaa aagtttatga tgaactgcat
    10261 aacgcccggg gcattgactt catctgggaa aatctggata cccaggagcg tgagcaacgc
    10321 aagtttgcta tccgcacggt tctgagtacg cagtatctga gggactaccc ggaaagcgtt
    10381 cttaagtcag ccaatactct ctggttactg cgctacaaac ccgaagatat ccccgtactg
    10441 agggataact tcaacgttcc tgaatttatg ctgaaacgct tcctgaaaat gccggaaggc
    10501 ccggcaccag atggtagtgg tgttcctgtt ctgggagtat tcagggtaaa aagtggcacg
    10561 cttgcgagaa tactcaaatt taccgtcggt ccactggagc tatgggcgct gaactcatcg
    10621 ccaaaagaca gtgccctgcg taaaacgctg acaaataagt taggttc
//
LOCUS       DAEEBB010000138        10447 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEEBB010000138 DAEEBB010000000
VERSION     DAEEBB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.138"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="KLF07_003395"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="KLF07_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBD9833332.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="KLF07_003396"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="KLF07_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBD9833333.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="KLF07_003397"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="KLF07_003397"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD9833334.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="KLF07_003398"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="KLF07_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="KLF07_003399"
     CDS             3565..3849
                     /locus_tag="KLF07_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD9833335.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="KLF07_003400"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="KLF07_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBD9833336.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="KLF07_003401"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="KLF07_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBD9833337.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="KLF07_003402"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="KLF07_003402"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD9833338.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="KLF07_003403"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="KLF07_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBD9833339.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10039..>10447
                     /locus_tag="KLF07_003404"
     CDS             10039..>10447
                     /locus_tag="KLF07_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD9833340.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT         2561 a         2690 c         2623 g         2573 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     9901 aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta ttttaaaaac
     9961 agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa
    10021 ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta
    10081 gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt
    10141 tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa
    10201 acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt
    10261 ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaac
    10321 aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct ggactatggt
    10381 cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc agaatttggt
    10441 ggcgata
//
LOCUS       DAEEBB010000139        10420 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEEBB010000139 DAEEBB010000000
VERSION     DAEEBB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.139"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="KLF07_003405"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="KLF07_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBD9833341.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="KLF07_003406"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="KLF07_003406"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBD9833342.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="KLF07_003407"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="KLF07_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBD9833343.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="KLF07_003408"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="KLF07_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBD9833344.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="KLF07_003409"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="KLF07_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="KLF07_003410"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="KLF07_003410"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBD9833345.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="KLF07_003411"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="KLF07_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBD9833346.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="KLF07_003412"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="KLF07_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBD9833347.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="KLF07_003413"
     CDS             complement(9457..10149)
                     /locus_tag="KLF07_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBD9833348.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2514 c         2752 g         2605 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc
//
LOCUS       DAEEBB010000140        10362 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEEBB010000140 DAEEBB010000000
VERSION     DAEEBB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.140"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..154
                     /locus_tag="KLF07_003414"
     CDS             <1..154
                     /locus_tag="KLF07_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD9833349.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="KLF07_003415"
     CDS             258..1172
                     /locus_tag="KLF07_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9833350.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="KLF07_003416"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="KLF07_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBD9833351.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="KLF07_003417"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="KLF07_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBD9833352.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="KLF07_003418"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="KLF07_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBD9833353.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="KLF07_003419"
     CDS             5467..7149
                     /locus_tag="KLF07_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD9833354.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="KLF07_003420"
     CDS             7201..8358
                     /locus_tag="KLF07_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD9833355.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="KLF07_003421"
     CDS             complement(8324..8446)
                     /locus_tag="KLF07_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833356.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="KLF07_003422"
     CDS             8655..10340
                     /locus_tag="KLF07_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBD9833357.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3029 a         2207 c         2213 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg ta
//
LOCUS       DAEEBB010000141        10340 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEEBB010000141 DAEEBB010000000
VERSION     DAEEBB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.141"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="KLF07_003423"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="KLF07_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBD9833358.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="KLF07_003424"
     CDS             complement(1127..1213)
                     /locus_tag="KLF07_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBD9833359.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="KLF07_003425"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="KLF07_003425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD9833360.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="KLF07_003426"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="KLF07_003426"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBD9833361.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="KLF07_003427"
     CDS             3562..3606
                     /locus_tag="KLF07_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833362.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="KLF07_003428"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="KLF07_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBD9833363.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="KLF07_003429"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="KLF07_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBD9833364.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="KLF07_003430"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="KLF07_003430"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBD9833365.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="KLF07_003431"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="KLF07_003431"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBD9833366.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="KLF07_003432"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="KLF07_003432"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBD9833367.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="KLF07_003433"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="KLF07_003433"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBD9833368.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2711 a         2717 c         2523 g         2389 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag
//
LOCUS       DAEEBB010000142         9957 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEEBB010000142 DAEEBB010000000
VERSION     DAEEBB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.142"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="KLF07_003434"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="KLF07_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBD9833369.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="KLF07_003435"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="KLF07_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBD9833370.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="KLF07_003436"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="KLF07_003436"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD9833371.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="KLF07_003437"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="KLF07_003437"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBD9833372.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="KLF07_003438"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="KLF07_003438"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBD9833373.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="KLF07_003439"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="KLF07_003439"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBD9833374.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="KLF07_003440"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="KLF07_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBD9833375.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="KLF07_003441"
     CDS             8251..8466
                     /locus_tag="KLF07_003441"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833376.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="KLF07_003442"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="KLF07_003442"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBD9833377.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2502 a         2646 c         2518 g         2291 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcg
//
LOCUS       DAEEBB010000143         9895 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEEBB010000143 DAEEBB010000000
VERSION     DAEEBB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.143"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="KLF07_003443"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="KLF07_003443"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD9833378.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="KLF07_003444"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="KLF07_003444"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBD9833379.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="KLF07_003445"
     CDS             1905..2177
                     /locus_tag="KLF07_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBD9833380.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="KLF07_003446"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="KLF07_003446"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBD9833381.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="KLF07_003447"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="KLF07_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBD9833382.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="KLF07_003448"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="KLF07_003448"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBD9833383.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="KLF07_003449"
     CDS             6190..6741
                     /locus_tag="KLF07_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBD9833384.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="KLF07_003450"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="KLF07_003450"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBD9833385.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="KLF07_003451"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="KLF07_003451"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBD9833386.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2471 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagt
//
LOCUS       DAEEBB010000144         9850 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEEBB010000144 DAEEBB010000000
VERSION     DAEEBB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.144"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="KLF07_003452"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="KLF07_003452"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBD9833387.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="KLF07_003453"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="KLF07_003453"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBD9833388.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="KLF07_003454"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="KLF07_003454"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD9833389.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="KLF07_003455"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="KLF07_003455"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD9833390.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="KLF07_003456"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="KLF07_003456"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD9833391.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="KLF07_003457"
     CDS             5058..6191
                     /locus_tag="KLF07_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD9833392.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="KLF07_003458"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="KLF07_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD9833393.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="KLF07_003459"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="KLF07_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBD9833394.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="KLF07_003460"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="KLF07_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBD9833395.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="KLF07_003461"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="KLF07_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBD9833396.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="KLF07_003462"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="KLF07_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBD9833397.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="KLF07_003463"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="KLF07_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBD9833398.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9850
                     /locus_tag="KLF07_003464"
     CDS             9762..>9850
                     /locus_tag="KLF07_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833399.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2504 c         2502 g         2327 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg
//
LOCUS       DAEEBB010000145         9644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEEBB010000145 DAEEBB010000000
VERSION     DAEEBB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.145"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..667)
                     /locus_tag="KLF07_003465"
     CDS             complement(<1..667)
                     /locus_tag="KLF07_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBD9833400.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="KLF07_003466"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="KLF07_003466"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBD9833401.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="KLF07_003467"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="KLF07_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBD9833402.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="KLF07_003468"
     CDS             complement(5698..7014)
                     /locus_tag="KLF07_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBD9833403.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="KLF07_003469"
     CDS             complement(7004..8761)
                     /locus_tag="KLF07_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBD9833404.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="KLF07_003470"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="KLF07_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAEEBB010000146         9621 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEEBB010000146 DAEEBB010000000
VERSION     DAEEBB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.146"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            223..2199
                     /locus_tag="KLF07_003471"
                     /pseudo
     CDS             223..2199
                     /locus_tag="KLF07_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="KLF07_003472"
     CDS             2192..3328
                     /locus_tag="KLF07_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD9833405.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="KLF07_003473"
     CDS             3325..5325
                     /locus_tag="KLF07_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBD9833406.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="KLF07_003474"
     CDS             5447..5908
                     /locus_tag="KLF07_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBD9833407.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="KLF07_003475"
     CDS             complement(5949..6419)
                     /locus_tag="KLF07_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBD9833408.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="KLF07_003476"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="KLF07_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBD9833409.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="KLF07_003477"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="KLF07_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBD9833410.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="KLF07_003478"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="KLF07_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9382..>9621
                     /locus_tag="KLF07_003479"
     CDS             9382..>9621
                     /locus_tag="KLF07_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD9833411.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2354 a         2531 c         2468 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
     9481 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
     9541 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
     9601 gagtctgctg acgagtggtt t
//
LOCUS       DAEEBB010000147         9574 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEEBB010000147 DAEEBB010000000
VERSION     DAEEBB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.147"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..158)
                     /locus_tag="KLF07_003480"
     CDS             complement(<1..158)
                     /locus_tag="KLF07_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HBD9833412.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPA"
     gene            complement(158..1084)
                     /gene="glsB"
                     /locus_tag="KLF07_003481"
     CDS             complement(158..1084)
                     /gene="glsB"
                     /locus_tag="KLF07_003481"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBD9833413.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1148..2536)
                     /gene="sad"
                     /locus_tag="KLF07_003482"
     CDS             complement(1148..2536)
                     /gene="sad"
                     /locus_tag="KLF07_003482"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBD9833414.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2637..3518
                     /locus_tag="KLF07_003483"
     CDS             2637..3518
                     /locus_tag="KLF07_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9833415.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3595..4710
                     /gene="yneK"
                     /locus_tag="KLF07_003484"
     CDS             3595..4710
                     /gene="yneK"
                     /locus_tag="KLF07_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBD9833416.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4860..6050
                     /gene="ydeA"
                     /locus_tag="KLF07_003485"
     CDS             4860..6050
                     /gene="ydeA"
                     /locus_tag="KLF07_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBD9833417.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6075..6740)
                     /gene="marC"
                     /locus_tag="KLF07_003486"
     CDS             complement(6075..6740)
                     /gene="marC"
                     /locus_tag="KLF07_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBD9833418.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6952..7386
                     /gene="marR"
                     /locus_tag="KLF07_003487"
     CDS             6952..7386
                     /gene="marR"
                     /locus_tag="KLF07_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBD9833419.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7406..7789
                     /gene="marA"
                     /locus_tag="KLF07_003488"
     CDS             7406..7789
                     /gene="marA"
                     /locus_tag="KLF07_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBD9833420.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7821..8039
                     /gene="marB"
                     /locus_tag="KLF07_003489"
     CDS             7821..8039
                     /gene="marB"
                     /locus_tag="KLF07_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBD9833421.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8096..9535)
                     /locus_tag="KLF07_003490"
     CDS             complement(8096..9535)
                     /locus_tag="KLF07_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBD9833422.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2467 a         2457 c         2162 g         2488 t
ORIGIN      
        1 gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct
       61 ttctccaggc aatactgacg ctctttcatg cccagacgaa agcgagagcg aaattttgaa
      121 cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgtcctaact
      181 gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt
      241 ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa
      301 cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac
      361 cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat
      421 caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac
      481 agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat
      541 ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa
      601 attcggaacg cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca
      661 cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca
      721 ccagcgcacc agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca
      781 ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt
      841 cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa
      901 aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc
      961 ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac
     1021 caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg
     1081 ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata
     1141 tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga
     1201 aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt
     1261 cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc
     1321 tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag
     1381 ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca
     1441 tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt
     1501 agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg
     1561 ttttctctac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag
     1621 tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt
     1681 cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct
     1741 gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat
     1801 cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg
     1861 ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag
     1921 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     1981 atacgccttg cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca
     2041 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     2101 cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg
     2161 gtcgatactc aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg
     2221 ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg
     2281 cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg
     2341 cgcgcagagc cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc
     2401 gccagtcgcg aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag
     2461 cccacggcag cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg
     2521 ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt
     2581 tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg
     2641 atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg
     2701 cagcaaaagt gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag
     2761 aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc
     2821 ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg
     2881 tcgctggcga tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag
     2941 tgcgcattcc ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac
     3001 tttccaccgg cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg
     3061 catttattga tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg
     3121 agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg
     3181 gcagtaacat ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt
     3241 ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt
     3301 tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta
     3361 tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa
     3421 cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac
     3481 tggatgtgtc tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg
     3541 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
     3601 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
     3661 gcaccacagg taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt
     3721 gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac
     3781 cttgataact tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat
     3841 gaaaaggttc agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt
     3901 gaatttcagg tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga
     3961 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
     4021 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg
     4081 ttcaaccctg gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg
     4141 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
     4201 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
     4261 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
     4321 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
     4381 gaagtctgca tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac
     4441 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     4501 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat
     4561 atggcgcgca ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac
     4621 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc
     4681 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     4741 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac
     4801 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     4861 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     4921 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     4981 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     5041 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     5101 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     5161 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     5221 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     5281 taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg
     5341 ggcagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc
     5401 ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa
     5461 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     5521 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     5581 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     5641 gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga
     5701 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     5761 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     5821 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     5881 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     5941 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     6001 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     6061 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     6121 tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc
     6181 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     6241 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     6301 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt
     6361 ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     6421 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg
     6481 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     6541 tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     6601 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     6661 agcgcgacag ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     6721 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     6781 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     6841 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     6901 tgcctgggca atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     6961 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     7021 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     7081 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     7141 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     7201 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     7261 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     7321 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     7381 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     7441 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     7501 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     7561 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     7621 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     7681 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     7741 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg
     7801 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     7861 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     7921 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     7981 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     8041 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa
     8101 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
     8161 gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt
     8221 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta
     8281 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
     8341 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
     8401 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
     8461 gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
     8521 ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa
     8581 aagacatgta gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat
     8641 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
     8701 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg
     8761 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac
     8821 agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
     8881 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg
     8941 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
     9001 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
     9061 ttgatgcgaa gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg
     9121 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct
     9181 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
     9241 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
     9301 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
     9361 tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc ccgtgagcgc
     9421 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
     9481 actgatgcgc ggctaccgcg ccaccccata aaaaaccttt tttaaatcct gacatacaca
     9541 actccttaac taaaatagat taccaccaga cttc
//
LOCUS       DAEEBB010000148         9542 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEEBB010000148 DAEEBB010000000
VERSION     DAEEBB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.148"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1321
                     /locus_tag="KLF07_003491"
     CDS             <1..1321
                     /locus_tag="KLF07_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD9833423.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="KLF07_003492"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="KLF07_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBD9833424.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /locus_tag="KLF07_003493"
     CDS             2703..3482
                     /locus_tag="KLF07_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBD9833425.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="KLF07_003494"
     CDS             3479..6418
                     /locus_tag="KLF07_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBD9833426.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="KLF07_003495"
     CDS             6351..6665
                     /locus_tag="KLF07_003495"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBD9833427.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="KLF07_003496"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="KLF07_003496"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="KLF07_003497"
     CDS             complement(7120..7608)
                     /locus_tag="KLF07_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBD9833428.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="KLF07_003498"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="KLF07_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBD9833429.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAEEBB010000149         9524 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEEBB010000149 DAEEBB010000000
VERSION     DAEEBB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.149"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..1095)
                     /locus_tag="KLF07_003499"
                     /pseudo
     CDS             complement(1..1095)
                     /locus_tag="KLF07_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(1123..1743)
                     /locus_tag="KLF07_003500"
     CDS             complement(1123..1743)
                     /locus_tag="KLF07_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBD9833430.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(1740..2621)
                     /gene="yciV"
                     /locus_tag="KLF07_003501"
     CDS             complement(1740..2621)
                     /gene="yciV"
                     /locus_tag="KLF07_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBD9833431.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            2759..2803
                     /gene="trpL"
                     /locus_tag="KLF07_003502"
     CDS             2759..2803
                     /gene="trpL"
                     /locus_tag="KLF07_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBD9833432.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    2780..2873
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            2895..4457
                     /gene="trpE"
                     /locus_tag="KLF07_003503"
     CDS             2895..4457
                     /gene="trpE"
                     /locus_tag="KLF07_003503"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBD9833433.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            4457..6052
                     /gene="trpD"
                     /locus_tag="KLF07_003504"
     CDS             4457..6052
                     /gene="trpD"
                     /locus_tag="KLF07_003504"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBD9833434.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            6053..7414
                     /gene="trpCF"
                     /locus_tag="KLF07_003505"
     CDS             6053..7414
                     /gene="trpCF"
                     /locus_tag="KLF07_003505"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBD9833435.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            7426..8619
                     /gene="trpB"
                     /locus_tag="KLF07_003506"
     CDS             7426..8619
                     /gene="trpB"
                     /locus_tag="KLF07_003506"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBD9833436.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            8619..9425
                     /gene="trpA"
                     /locus_tag="KLF07_003507"
     CDS             8619..9425
                     /gene="trpA"
                     /locus_tag="KLF07_003507"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBD9833437.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         2255 a         2538 c         2489 g         2242 t
ORIGIN      
        1 cgccacccgc gccgccacca gcaccaccac cggaataact tcgccccaac aaactggttt
       61 tggcccccag actcaggtgc atctgtaatg aaaacaaaac gccactacgg gcaatttccg
      121 gggcgctaac agccgctgtg agtgatgggg tgatacgtgc tacccattct cttccaaggt
      181 ttgctgagac tgcccaacct agcaacgcgt aatccagttt ccgtgtttcc cctttccgcc
      241 gacgcccact gtgtgttgct gcctctttac gcgcaagata gttaacgata gcttcaccgt
      301 gggcataacg ttgctgccca gtttgtgtaa actttggcag aaaaatatac cccatgccag
      361 tgagataacc ggtcaggaaa acagcgctaa aataacccgc tggcatatac cagtaaaaga
      421 atacatctcc gacattgatg tagagagaat agaaggccac gcctgaacaa atcagtggta
      481 aacaattctt ccgtcctcaa ggatgcggcc atttcgatat gtgtcgccct cttctccagt
      541 aaagagatcg atttcgctaa atggattacc cgcgatatca gggaacttga gtgaaaatcg
      601 ggtcttatag atttcaaacg gccagtggtt accagtcacg ttccagatta acagatctga
      661 atctccctca cgcaggaaag catttttaac ctgataatga atttgataat gatagtcgcc
      721 ttgaggcaag aaacgttgtt tatcgcccac gacaatactc attaacccaa tatgccagtc
      781 atgatgccag ggttcaggaa taccatcacg ggtgacgcca acaatatgat agttcagcag
      841 tgcagcgtcc ccaccaggct gcatccagag tcgtggtaaa tcacgataaa atccgtgttt
      901 aatctgctga tttcgcgcct gaaaatgaat attttctttg atgtcgagca ccccatccgg
      961 ttgcagagtg gcttcaacat caaagagcga aatcccttct tcataagaag ggatctccgt
     1021 gttttctgca taacttagag atgatacaaa tagccctgct atcaacagca aaatgcagcg
     1081 aaacttccct gccatagtct tccatccgtg gaacgcaatt tgttataaga aaggcttcac
     1141 atcacctacg ccttcacgca ccacgaccgg cgtatcatcg gtaagatcaa taaccgttgt
     1201 cggtttctgc ccgagatagc cgccgtgaat aatcaaatct acctgttttt ccagacggtc
     1261 tttaatttct tcgggatcgg attcggtaaa ttcgctgcct ggcagcatta gcgaagtgga
     1321 aagcatcggt tcgcccagtg cttcaagcaa cgcctgcgca atcgggttag aaggcacacg
     1381 cataccgatg gttttgcgtt tttcctgcaa cagacggcgt ggcacttcct tcgtcccctt
     1441 caggatgaag gtatagttgc ccggcgtgtt gtttttcatt aaacgaaacg caacgttatc
     1501 aacaaatgaa taggtcgaga gttcagaaag atcgcgacac atcagggtaa agttgtgacc
     1561 gtccggcagc tggcgaatac gacaaatacg ctccatcgcg tttttatctt caattttaca
     1621 gccgagcgca tagccggaat cagttggata aacaatcacc ccgcctttac gcacgatctc
     1681 caccgcctgg ttgatcagac gttgctgtgg gttatcagga tgaatataaa aaaactggct
     1741 cataattccc tctctgtggt gttctgcggc tgttcccata gctgccagac gccttcaacg
     1801 cctgcgggca gccagagttt acgacctagt tcaatccacg ggcatggctg atgaaaatca
     1861 gatccttgtg atgcccataa atgatgctga cgcgcaaggg ccgccagctg ggtacgttca
     1921 ttgggcgatt gctggcactg cgcgacttcc atcgcgtcac cgtggtgttc ggcaaaatgc
     1981 gcaaccaatc ttttcagcca tttagcacta agattgtacc gcccaggatg agctagcacc
     2041 gccttaccgc cagaatgatg aatgacatca atagcttgtt ctattgtaca ccactgtggc
     2101 ggaacgtatc cggttttccc gcgcgccaga tattttttaa agacatccgc cattgaactg
     2161 gctttgccgc accccacaag gaaacgcgca aaatgaccgc gcgtcaccgc accgccctgc
     2221 gccagtcgtc gcgcgccttc cagtgcccct ggtatttgcg ctttttcaag tctttcggca
     2281 atcagttgcg cccgctgatt gcgccgttct gtctgctgcg cgaggaactc acacattagc
     2341 ggatgagtaa tatcaatatt cagcccgaca atatgaattt catgattttc ccagaccgtg
     2401 gaaatttcca cgccgggaat aagattcagc gccagtcccg aacgtgaaat ttcctctctt
     2461 gctggcacga ttgcagctgt gttgtcatgg tcggtgatcg ccagggtacc gacgcgcatc
     2521 tcgactgcac ggtgcaccaa tacttctggc gtcaggcagc catcggaagc tgtggtatgg
     2581 ctgtgcaggt cgtaaatcac tgcgtaattc gtgtcgctca aggcgcactc ccgttctgga
     2641 taatgttttt tgcgccgaca tcataacagt tctggcaaat attctgaaat cagctgttga
     2701 caattaatca tcgaactagt taactagtgc gcaagttcac gtaaaaaggg tatcgacaat
     2761 gaaagcaatt ttcgtactga aagcttggtg gcgcacttcc tgaaacgggc agtgtattca
     2821 ccatgcgtaa agcaatcaga tacccagccc gcctaatgag cgggcttttt tttgaacaaa
     2881 attagagaat aacaatgcaa acacaaaaac cgactctcga actgctaacc tgcgaaggcg
     2941 cttatcgcga caacccgact gcgctttttc accagttgtg tgggaatcgt ccggcaacgc
     3001 tgctgctgga atccgcagat atcgacagca aagatgattt aaaaagcctg ctactggtag
     3061 acagtgcgct gcgcattaca gcattaggtg acactgtcac aattcaggca ctttccggca
     3121 acggcgaagc cctgctggca ctactggata acgccctgcc tgcgggtgtg gaaaatgaac
     3181 aatcaccaaa ctgccgtgtg ctgcgcttcc cccctgtcag tccactgctg gatgaagacg
     3241 cccgcttatg ctccctttcg gtttttgacg ctttccgttt attgcagaat ctgttgaatg
     3301 taccgaagga agaacgagaa gcaatgttct tcggcggcct gttctcttat gaccttgtgg
     3361 cgggatttga agatttaccg caactgtcag cggaaaataa ctgccctgat ttctgttttt
     3421 atctcgctga aacgctgatg gtgattgacc atcagaaaaa aaacacccgc attcaggcca
     3481 gcctgtttgc tccgaatgaa gaagaaaaac aacgtctcac tgctcgcctg aacgatcttc
     3541 gccagcagct gaccgaagcc gcgccgccgc tgccggtggt ttccgtgccg catatgcgtt
     3601 gtgaatgtaa tcagagcgat gaagagttcg gtggcgtagt gcgtttgttg caaaaagcga
     3661 ttcgcgctgg agaaattttc caggtggtgc catctcgccg tttctctctg ccctgcccgt
     3721 caccgctggc ggcctattac gtgctgaaaa agagtaatcc cagcccgtac atgtttttta
     3781 tgcaggataa tgatttcacc ctgtttggcg cgtcgccgga aagttcgctc aagtatgacg
     3841 ccaccagccg ccagattgag atctacccga ttgccggaac acgtccacgc ggtcgtcgtg
     3901 ccgatggctc gctggacaga gacctcgaca gccgcatcga actggaaatg cgtaccgatc
     3961 ataaagagct ttctgaacat ctgatgctgg tggatctcgc ccgtaatgat ctggcacgca
     4021 tttgcacccc cggcagcagc tacgtcgccg atcttaccaa agttgaccgt tactcttacg
     4081 tgatgcacct ggtctcccgc gtggtcggtg agctgcgcca cgatctcgac gccctgcacg
     4141 cttaccgcgc ctgtatgaat atggggacgt taagcggtgc gccgaaagta cgcgccatgc
     4201 agttaattgc cgaggcggaa ggtcgtcgcc gcggcagcta cggcggcgcg gtaggttatt
     4261 tcaccgcgca tggcgatctc gacacctgca ttgtgatccg ctcggcgctg gtggaaaacg
     4321 gtatcgccac cgtgcaagcc ggtgctggcg tagtccttga ttctattccg cagtcggaag
     4381 ccgacgaaac ccgtaataaa gcccgcgctg tactgcgcgc tattgccacc gcgcatcatg
     4441 cacaggagac tttctgatgg ctgacattct gctgctcgat aatatcgact cttttacgta
     4501 caacctggca gatcagttgc gcagcaatgg gcataatgtg gtgatttacc gcaaccatat
     4561 tccggcgcag accttaattg aacgcctggc gacgatgagc aatccgatac tgatgctttc
     4621 tcctggcccc ggtgtgccga gcgaagccgg ttgtatgccg gaactcctca cccgcttgcg
     4681 tggcaagctg cccattattg gcatttgcct cggacatcag gcaattgtcg aagcttacgg
     4741 gggctatgtc ggtcaggcgg gcgaaattct tcacggtaaa gcgtcgagca ttgaacatga
     4801 cggtcaggcg atgtttgccg gattaacaaa cccgctgccg gtggcgcgtt atcactcgct
     4861 ggttggcagt aacattccgg ccggtttaac catcaacgcc cattttaatg gcatggtgat
     4921 ggcagtacgt cacgatgcgg atcgcgtttg tggattccag ttccatccgg aatccattct
     4981 caccacccag ggcgctcgcc tgctggaaca aacgctggcc tgggcgcagc agaaactaga
     5041 gccagccaac acgctgcaac cgattctgga aaaactgtat caggcgcaga cgcttagcca
     5101 acaagaaagc caccagctgt tttcagcggt ggtgcgtggc gagctgaagc cggaacaact
     5161 ggcggcggcg ctggtgagca tgaaaattcg cggtgagcac ccgaacgaga tcgccggggc
     5221 agcaaccgcg ctactggaaa acgcagcgcc gttcccgcgc ccagattatc tgtttgctga
     5281 tatcgtcggt actggcggtg acggcagcaa cagtatcaat atttctaccg ccagtgcgtt
     5341 tgtcgccgct gcctgtgggc tgaaagtggc gaaacacggc aaccgtagcg tctccagtaa
     5401 atctggttcg tccgatctgc tggcggcgtt cggtattaat cttgatatga acgccgataa
     5461 atcgcgccag gcgctggatg agttaggtgt ttgtttcctc tttgcgccga agtatcacac
     5521 cggattccgc cacgcgatgc cggttcgcca gcaactgaaa acccgcaccc tgttcaatgt
     5581 gctggggcca ttgattaacc cggcgcatcc gccgctggcg ttaattggtg tttatagtcc
     5641 ggaactggtg ctgccgattg ccgaaacctt gcgcgtgctg gggtatcaac gcgcggcggt
     5701 ggtgcacagc ggcgggatgg atgaagtttc attacacgcg ccgacaatcg ttgccgagct
     5761 gcatgacggc gaaattaaga gctatcaatt gaccgctgaa gattttggcc tgactcccta
     5821 ccaccaggag caactggcag gcggaacacc ggaagaaaac cgtgacattt taacacgctt
     5881 gttacaaggt aaaggcgacg ccgcccatga agcagccgtc gctgcgaacg tcgccatgtt
     5941 aatgcgcctg catggccatg aagatctgca agccaatgcg caaaccgttc ttgaggtact
     6001 gcgcagtggt tccgcttacg acagagttac cgcgctggcg gcacgagggt aaatgatgca
     6061 aaccgtttta gcgaaaatcg tcgcagacaa ggcgatttgg gtagaaaccc gcaaacagca
     6121 gcaaccgctg gccagttttc agaatgaggt tcagccgagc acgcgacatt tttatgatgc
     6181 gctacagggt gcacgcacgg cgtttattct ggagtgcaag aaagcgtcgc cgtcaaaagg
     6241 cgtgatccgt gatgatttcg atccggcacg cattgcctcc atttataaac attacgcttc
     6301 ggcaatttcg gtgctgactg atgagaaata ttttcagggg agctttgatt tcctccccat
     6361 cgtcagccaa atcgccccgc agccgatttt atgtaaagac ttcattatcg acccttacca
     6421 gatctatctg gcgcgctatt accaggccga tgcctgctta ttaatgcttt cagtactgga
     6481 tgacgaccaa tatcgccagc ttgcagccgt cgctcacagt ctggagatgg gggtgctgac
     6541 cgaagtcagt aatgaagagg aactggagcg cgccattgca ttgggggcaa aggtcgttgg
     6601 catcaacaac cgcgatctgc gtgatttgtc gattgatctc aaccgtaccc gcgagcttgc
     6661 gccgaaactg gggcacaacg tgacggtaat cagcgaatcc ggcatcaata cttacgctca
     6721 ggtgcgcgag ttaagccact tcgctaacgg ttttctgatt ggttcggcgt tgatggccca
     6781 tgacgatttg cacgccgccg tgcgccgggt gttgctgggt gagaataaag tatgtggtct
     6841 gacgcgtggg caagatgcta aagcagctta tgacgcgggc gcgatttacg gtgggttgat
     6901 tttggttgcg acatcaccgc gttgcgtcaa cgttgaacag gcgcaggaag tgatggctgc
     6961 ggcaccgttg cagtatgttg gcgtgttccg caatcacgat attgccgatg tggtggacaa
     7021 agctaaggtg ttatcgctgg cggcagtgca actgcatggt aatgaagatc agctgtatat
     7081 cgatacgctg cgtgaagctc tgccagcaca cgttgccatc tggaaagcat taagcgtcgg
     7141 tgaaaccctg cccgcccgcg agcttcagca cgttgataaa tatgttttag acaacggcca
     7201 gggtggaagc gggcaacgtt tcgactggtc actattaaat ggtcaatcgc ttggcaacgt
     7261 tctgctggcg gggggcttag gcgcagataa ctgcgtggaa gcggcacaaa ccggctgcgc
     7321 cggacttgat tttaattctg ctgtagagtc gcaaccgggc atcaaagacg cacgtctttt
     7381 ggcctcggtt ttccagacgc tgcgcgcata ttaaggaaag gaacaatgac aacattactt
     7441 aacccctatt ttggtgagtt tggcggcatg tacgtgccac aaatcctgat gcctgctctg
     7501 cgccagctgg aagaagcttt tgtcagcgcg caaaaagatc ctgaatttca ggcccagttc
     7561 aacgacctac tgaaaaacta tgccgggcgt ccaaccgcac tgaccaaatg ccagaacatt
     7621 acggctggca cgaacaccac gctgtatctg aagcgcgaag atttgctgca cggcggcgcg
     7681 cataaaacta accaggtgct cggtcaggct ttactggcga agcggatggg taaaaccgaa
     7741 atcatcgccg aaaccggtgc cggtcagcat ggcgtggcgt cggcccttgc cagcgccctg
     7801 ctcggcctga aatgccgtat ttatatgggt gccaaagacg ttgaacgcca gtcgcctaac
     7861 gtttttcgta tgcgcttaat gggtgcggaa gtgatcccgg tgcatagcgg ttccgcgacg
     7921 ctgaaagatg cctgtaacga ggcgctgcgc gactggtccg gtagttacga aaccgcgcac
     7981 tatatgctgg gcaccgcagc tggcccgcat ccttatccga ccattgtgcg tgagtttcag
     8041 cggatgattg gcgaagaaac caaagcgcag attctggaaa gagaaggtcg cctgccggat
     8101 gccgttatcg cctgtgttgg cggcggttcg aatgccatcg gcatgtttgc tgatttcatc
     8161 aatgaaacca acgtcggcct gattggtgtg gagccaggtg gtcacggtat cgaaactggc
     8221 gagcacggcg caccgctaaa acatggtcgc gtgggtatct atttcggtat gaaagcgccg
     8281 atgatgcaaa ccgaagacgg gcagattgaa gaatcttact ccatctccgc cggattggat
     8341 ttcccgtctg tcggcccaca acacgcgtat cttaacagca ctggacgcgc tgattacgtg
     8401 tctattaccg atgatgaagc ccttgaagcc ttcaagacgc tgtgcctaca cgaagggatc
     8461 atcccggcgc tggaatcctc ccacgccctg gcccatgcgc tgaaaatgat gcgcgaaaac
     8521 ccggaaaaag agcagctact ggtggttaac ctttccggtc gcggcgataa agacatcttc
     8581 accgttcacg atattttgaa agcacgaggg gaaatctgat ggaacgctac gaatctctgt
     8641 ttgcccagtt gaaggagcgc aaagaaggcg cattcgttcc tttcgtcacg ctcggggatc
     8701 cgggcattga gcagtcattg aaaattatcg atacgctaat tgaagccggt gctgacgcgc
     8761 tggagttagg catccccttc tccgacccac tggcagatgg cccgacgatt caaaacgcca
     8821 ctctgcgcgc ctttgcggca ggtgtgactc cggcacaatg ttttgaaatg ctggcactga
     8881 ttcgccagaa acacccgacc attcccattg gcctgttgat gtatgccaat ctggtgttta
     8941 acaaaggcat tgatgagttt tatgcccagt gcgagaaagt cggcgtcgat tcggtgctgg
     9001 ttgccgatgt gccaattgaa gagtccgcgc ccttccgcca ggccgcgttg cgccataatg
     9061 tcgcacctat cttcatctgc ccgccaaatg ccgatgacga cctgctgcgc cagatagcct
     9121 cttacggtcg tggttacacc tatttgctgt cacgagcggg cgtgaccggc gcagaaaacc
     9181 gcgccgcgtt acccctcaat catctggttg cgaagctgaa agagtacaac gctgcacctc
     9241 cattgcaggg gtttggtatt tccaccccgg atcaggtaaa agcagcgatt gatgcaggag
     9301 ctgcgggcgc gatttctggt tcagccattg ttaaaatcat cgagcaacat attaatgagc
     9361 cagagaaaat gctggcggca ctgaaagttt ttgtacaacc gatgaaagtg gcgacgcgca
     9421 gttaatcccc ctgccgccag ttccgctggc ggcattttaa ctttctttaa cgaagccgga
     9481 aaaatcctaa attcatttaa tatttatctt tttaccgttt cgct
//
LOCUS       DAEEBB010000150         9500 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEEBB010000150 DAEEBB010000000
VERSION     DAEEBB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.150"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(149..1231)
                     /locus_tag="KLF07_003508"
     CDS             complement(149..1231)
                     /locus_tag="KLF07_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBD9833438.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="KLF07_003509"
     CDS             complement(1228..3024)
                     /locus_tag="KLF07_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBD9833439.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="KLF07_003510"
     CDS             complement(3275..3631)
                     /locus_tag="KLF07_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833440.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="KLF07_003511"
     CDS             complement(3689..4111)
                     /locus_tag="KLF07_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD9833441.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="KLF07_003512"
     CDS             complement(4127..4852)
                     /locus_tag="KLF07_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HBD9833442.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="KLF07_003513"
     CDS             complement(4874..5620)
                     /locus_tag="KLF07_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD9833443.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="KLF07_003514"
     CDS             complement(5627..6691)
                     /locus_tag="KLF07_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HBD9833444.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="KLF07_003515"
     CDS             complement(6763..7188)
                     /locus_tag="KLF07_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD9833445.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="KLF07_003516"
     CDS             complement(7185..7448)
                     /locus_tag="KLF07_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9833446.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="KLF07_003517"
     CDS             7560..8060
                     /locus_tag="KLF07_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD9833447.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="KLF07_003518"
     CDS             8080..8274
                     /locus_tag="KLF07_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBD9833448.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="KLF07_003519"
     CDS             8274..8564
                     /locus_tag="KLF07_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD9833449.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="KLF07_003520"
     CDS             8844..8996
                     /locus_tag="KLF07_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBD9833450.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8996..9373
                     /locus_tag="KLF07_003521"
     CDS             8996..9373
                     /locus_tag="KLF07_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833451.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2444 a         2492 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac
//
LOCUS       DAEEBB010000151         9397 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEEBB010000151 DAEEBB010000000
VERSION     DAEEBB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.151"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(350..1603)
                     /locus_tag="KLF07_003522"
     CDS             complement(350..1603)
                     /locus_tag="KLF07_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833452.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="KLF07_003523"
     CDS             1710..2603
                     /locus_tag="KLF07_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9833453.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="KLF07_003524"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="KLF07_003524"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBD9833454.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="KLF07_003525"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="KLF07_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD9833455.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="KLF07_003526"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="KLF07_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBD9833456.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="KLF07_003527"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="KLF07_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBD9833457.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="KLF07_003528"
     CDS             complement(6839..7693)
                     /locus_tag="KLF07_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD9833458.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="KLF07_003529"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="KLF07_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD9833459.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="KLF07_003530"
     CDS             complement(8323..>9397)
                     /locus_tag="KLF07_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD9833460.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2438 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAEEBB010000152         9212 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEEBB010000152 DAEEBB010000000
VERSION     DAEEBB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.152"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            73..732
                     /locus_tag="KLF07_003531"
     CDS             73..732
                     /locus_tag="KLF07_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBD9833461.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="KLF07_003532"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="KLF07_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBD9833462.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="KLF07_003533"
     CDS             complement(1860..2570)
                     /locus_tag="KLF07_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBD9833463.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="KLF07_003534"
     CDS             3004..3294
                     /locus_tag="KLF07_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBD9833464.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="KLF07_003535"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="KLF07_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBD9833465.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="KLF07_003536"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="KLF07_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBD9833466.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="KLF07_003537"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="KLF07_003537"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBD9833467.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="KLF07_003538"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="KLF07_003538"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBD9833468.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="KLF07_003539"
     CDS             complement(7538..7837)
                     /locus_tag="KLF07_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBD9833469.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="KLF07_003540"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="KLF07_003540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBD9833470.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="KLF07_003541"
     CDS             complement(9049..>9212)
                     /locus_tag="KLF07_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833471.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAEEBB010000153         9185 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEEBB010000153 DAEEBB010000000
VERSION     DAEEBB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.153"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="KLF07_003542"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="KLF07_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="KLF07_003543"
     CDS             complement(1751..2509)
                     /locus_tag="KLF07_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBD9833472.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="KLF07_003544"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="KLF07_003544"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBD9833473.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="KLF07_003545"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="KLF07_003545"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBD9833474.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="KLF07_003546"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="KLF07_003546"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBD9833475.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="KLF07_003547"
     CDS             complement(5474..6079)
                     /locus_tag="KLF07_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833476.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="KLF07_003548"
     CDS             complement(6082..6849)
                     /locus_tag="KLF07_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833477.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="KLF07_003549"
     CDS             complement(6852..7562)
                     /locus_tag="KLF07_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833478.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="KLF07_003550"
     CDS             complement(7578..9083)
                     /locus_tag="KLF07_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBD9833479.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2260 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctt
//
LOCUS       DAEEBB010000154         9074 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEEBB010000154 DAEEBB010000000
VERSION     DAEEBB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.154"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..963)
                     /gene="istA"
                     /locus_tag="KLF07_003551"
     CDS             complement(<1..963)
                     /gene="istA"
                     /locus_tag="KLF07_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBD9833480.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
     gene            complement(1050..1411)
                     /locus_tag="KLF07_003552"
                     /pseudo
     CDS             complement(1050..1411)
                     /locus_tag="KLF07_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1751..2916)
                     /locus_tag="KLF07_003553"
     CDS             complement(join(1751..2655,2655..2916))
                     /locus_tag="KLF07_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9833481.1"
                     /translation="MTKTVSTSKKTRKQHSPEFRSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILPKGRDILCEAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQFRQHCDSVVLAAAFTRS
                     KQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENL
                     LEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRLTAQLACDA
                     LQMALWRRKRPRNVIVHSDRGSQYCSADYQALLKWHNLRGSMSAKGCCYDNACVESFF
                     HSLKVECLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            3104..3226
                     /locus_tag="KLF07_003554"
                     /pseudo
     CDS             3104..3226
                     /locus_tag="KLF07_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3488..4054)
                     /gene="ipgB2"
                     /locus_tag="KLF07_003555"
     CDS             complement(3488..4054)
                     /gene="ipgB2"
                     /locus_tag="KLF07_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBD9833482.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(4337..4651)
                     /locus_tag="KLF07_003556"
     CDS             complement(4337..4651)
                     /locus_tag="KLF07_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833483.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(5163..5840)
                     /gene="ospD1"
                     /locus_tag="KLF07_003557"
     CDS             complement(5163..5840)
                     /gene="ospD1"
                     /locus_tag="KLF07_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBD9833484.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKSNIINVTNSAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(6211..6348)
                     /locus_tag="KLF07_003558"
                     /pseudo
     CDS             complement(6211..6348)
                     /locus_tag="KLF07_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(6427..6633)
                     /locus_tag="KLF07_003559"
     CDS             complement(6427..6633)
                     /locus_tag="KLF07_003559"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833485.1"
                     /translation="MDAENETVLNANMTHHLGCEKNQLRSGSNSRNGCLTKIITTGDE
                     PLEIRTLRDRNGTFEPQQLKKNQP"
     gene            complement(7317..7476)
                     /locus_tag="KLF07_003560"
                     /pseudo
     CDS             complement(7317..7476)
                     /locus_tag="KLF07_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7480..8634)
                     /locus_tag="KLF07_003561"
                     /pseudo
     CDS             complement(7480..8634)
                     /locus_tag="KLF07_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8654..9001)
                     /gene="tnpB"
                     /locus_tag="KLF07_003562"
     CDS             complement(8654..9001)
                     /gene="tnpB"
                     /locus_tag="KLF07_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD9833486.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(9001..>9074)
                     /locus_tag="KLF07_003563"
     CDS             complement(9001..>9074)
                     /locus_tag="KLF07_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD9833487.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
BASE COUNT         2272 a         2170 c         2052 g         2580 t
ORIGIN      
        1 gtcgaagctc accagatttt caccaggatg cacgtcatac tcttttttct ccggaggcag
       61 tgccagcatg gactgctgct cttcgagcca gcgatcacag ggacgggcct ggattgtttc
      121 atgctttcgt tggttagcga catcgtgcag ccagcgcaga ccgtggcggt tggctgtttc
      181 aacatcgaca gtgctcccca tcgggcgcag gcgagtcatt agtgggatgt aaaaactgtt
      241 acgggtgtac tgcaccatcc gttccacctt acctttagtc tgtgccctga aggggcgaca
      301 cagtcgggga gagaagccca tctccttgcc gaactgccac agcgaaggat ggaaccggtg
      361 ctgaccggtc tgatatgcgt cacgttgcag aaccacagtt ttcatattgt catacaacac
      421 ttcgcgcggc acaccaccaa agaagcggaa cgcattacga tggcaggtct ccagcgtgtc
      481 ataacgcata ttgtcagtga attcgatgta caacattcgg ctgtatccgg gaacagcaac
      541 gaacacgtga agcggtgagc gaccattacg catagtgccc cagtcaacct gcatctgtcg
      601 tccgggttca gtttcgaacc gaacggcagg ctcctgctcc tgaggaaccg agagagaacg
      661 aatgaatgcc ctgagaatgg tcattccgcc acgatatccc tggtctctga tctcgcgagc
      721 gattaccgtt gccgggattt tgtaaggatg agcatcggcg atgcgttgac gaatataatc
      781 ccggtattca tccaggagtg aagcaacagc aggtcgcggc gtatattttg gcggctcaga
      841 ttttgcctgc aaataacgtt taacggtatt gcgggagagc cccagttctc tggcaatcgc
      901 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
      961 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
     1021 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
     1081 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
     1141 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
     1201 tacgggcagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
     1261 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
     1321 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
     1381 aacgggggca atgaaggtta actgtgacca tgaaagaacc ttaaaagcgg gtattataac
     1441 ccctaattca actaattgga ggcattgtca tttttaatct atggatacac ttaaaaatcc
     1501 tctcacctta cgttaaaaac cttctcatgt ttagagtttt ttattatgtt tatcataatg
     1561 atatgacgat ctgagttttt aaaatcaaga agaaaagata agttttttaa atgcacagtg
     1621 cctgtggata actttgcgta aatccggagg acagatcact ctgaagcaag acaacagcat
     1681 ctgaaaacaa tatgctcacg tttcaaccac agggcgtaag tgatcctacc cacgtaatat
     1741 ggacacaggc ctaagcgagg ttctggtttt caaattgttc cggactgagg ccgccacacc
     1801 aactgtgccg tcgccagcga ttgtaatcac attcgatata attaaacact gttgcccgca
     1861 ttatttcccg gctgataaag tgttctccgt ggagacattc cactttcagc gaatgaaaga
     1921 agctttccac gcaggcatta tcgtagcagc agccctttgc gctcatactg ccacgcagat
     1981 tatgccactt cagcagcgcc tgataatctg ctgaacagta ctgacttcct cggtccgagt
     2041 gaacgataac gttccggggt ctcttacgcc gccacagcgc catctgcagg gcatcgcagg
     2101 ccagttgcgc cgtcaggcgt ggtgacatgg accagccaat aacggcacgt gaccacaggt
     2161 caatgaccac agccagatac agccagcctt catctgtacg taagtacgtg atgtctcccg
     2221 cccacttctg gttcggacca ctggcgtaaa aatcctgctc cagcagattt tctgacacag
     2281 gcaggccatg tgcgcggtaa ctgaccgggc tgaacttccg ggaggccttt gccctcagcc
     2341 cctgacggcg caggcttgcc gccacggttt ttacgttaaa gtggtaaccc tgagcacgca
     2401 gttcatccgt caggcgtggt gcaccgtaac gctgttttga ccgggtaaag gccgccgcga
     2461 ggacaacgct gtcgcagtgc tgacggaact gctgacgagg gcttatccct gtccgccgct
     2521 gacaccacgt ataccagccg ctgcgggcca cccggagcac gcggcacatt gccttgatgc
     2581 tgaactcagc ctgatgtttt tcaataaaga catacttcat ttcaggcgct tcgcaaagta
     2641 tgtcgcggcc ttttggagga tagccagttc ctctgcctgc tccgccagtt gccgcttcag
     2701 gcgggcattt tcagcggcca gttcactttc acgctcagac gaggtcatct gttgctgcag
     2761 tttgctgcgc cacgcataga gctgagattc atacaggctg agttcacggg ctgcggcagc
     2821 aacaccgatg cgttcagcaa gcttcagggc ttcactgcga aattcaggcg aatgctgttt
     2881 acgtgttttt ttgctggttg atactgtttt tgtcatgtga gtcacctctg actgagagtt
     2941 tactcactta gccgcgtgtc cactattgct gggtaagatc aatttgctca ctggtattta
     3001 ctacgtgcat cgaatgagtg atcaagagcc gtatgacgcg agagtgtcac gtacggttcc
     3061 gtgagcagct ggagagtgaa actctctccg gctgactcga ctcgcaggtt ccgacagcgg
     3121 tgacgaacat gcagcggtgt tgttctcgct gatcggcaca cgccgtctga acaatgtgga
     3181 gccagaaaaa tggctgcgtt acgttattga acatatccag gactgaccgg caaaccgggt
     3241 acgcgatctg ttgccttgga aagttgatct gacttctcag taaatatcaa tatgattctg
     3301 acgagccgct tacctttgag cagagaaagg ggagagggat cccacatcca tgagctagga
     3361 aaatacaaaa actggctata caacaattca atatattagg acttacttgc gaggagaaat
     3421 atagcaccgc tcaggcaatt cctgggtatt tacttttctt agtttcttta ctcgggatgt
     3481 tctgtcatca gaaaggcgat tctaaatttg taatatagtc actcatattc ccacgtaatt
     3541 tctttagatc aatatattgc acatttttct caaaaaactg atcggaaata agatttgcaa
     3601 cattgcttgt tgtatcattt ttaacgcttc tattcatccc ttcacaaagt tcgtccattt
     3661 tctttttgaa ataactgctt cctgatacaa gatgatttat ggaactttgt gcagcattga
     3721 tgtctagaga aatgcgataa tctttctcaa cctgtttaaa tatctgttta cggaggttat
     3781 cgccgatttc tcggctgtgt tctgcacaaa acttatcaat acattggttt accacccggg
     3841 atacgtagac ttttttttca tcatttttcc aatctgtaat attttcaccg atttgttctg
     3901 aaacagagct caaaatacta gtatttattt tggttgatga tatttttact atccttttcc
     3961 ccattgtaca gcggataatc tcatccacct ttcctgaaaa atatcttttg tgagataaac
     4021 tgattccaaa attattaaaa gatgttccaa gcattataat cactttgtca tgtaaaaata
     4081 gtaacattgt cattatttta gctgattcaa acaattctgt aaatgctttg agttctttac
     4141 tcccttggct caatgatgga ccgtctggaa tcccagagac taaactctct gagaaagaga
     4201 gtatgaaaaa acttggggca gtccctaaat gaagggacca acagcggtaa aataaccggc
     4261 atcattcata atgatgccct gttagttgta gttatgaaga tttacgtcat taaaattgct
     4321 gttcgtagtg tcagtcctat ggtctggcat cagctgagga tcgtagccga tacatcactg
     4381 gcttcacgac attcgtgttg aggctattca tgaaaactcc acgctgaaaa cgccgttttg
     4441 tataaacggt cacggtatgc ccggagccac tgttgctgat gaattcgata aaaccttggc
     4501 gtttcttgaa gctatcgtta atgcggataa tgaaacaacg attggtgaga ttcgctcttt
     4561 tgccgacgcc ctggacgctg tccggttcaa tcgcaacaaa atcaaccggc agctgagcaa
     4621 acccaacctt gcctctctgg cgctggagca tgaagtgatt tggctgggac gtagccgctg
     4681 acagcttcta taaaagtatg atcctaccca cgtaatatga acacaggctt aagtgaggtt
     4741 ctggttttca aattgttcag gactgagacc tccacaccaa ctgtgccgtc gtcagcgatt
     4801 gtaatcgcac ttgatataat taaacactgt atatttcatt tgtggagtct ccagtctgag
     4861 ggggctttat gcattccgta ttgggcacta tgatgccaga aatctgcgag gctccacacc
     4921 ttcctgtatt tcccttcctc tatcacagtg cactttactt aatagggtgg gatgtgttca
     4981 ttacatttat tgtgtttttt atgattgttt ttgcgtccag tgtcgttcgt catgggaaac
     5041 tggttctatg ggtagtatcg ctcagtctag ttaactgttt gtttcttttt agaaattaag
     5101 cagatcacac aattcgggga gagatccttc taagtgcaat tctttgcagc tatttagcac
     5161 acttaaaaca actcttgtat tttgtcagaa ataatattaa acatgtcttg ttcattattg
     5221 ttaagtctgt ctgctatttc tgtaagtttt atcccactat gatggcatat atttatatat
     5281 gccatcatag cctcgatatt ccctttccct gcggccagaa ataatcctgg taaaccctca
     5341 ttatctctta catccaatag tttaatcgtt tcttttggtg ataaatcaaa agttttaata
     5401 aaatctccat aagcttgaat cgcagcggaa tgaccttctt gaagagctat gaacaaagca
     5461 gattccccag cattatttcg tgctgcaaga acttctagtt tttcctcttt gggtatttcg
     5521 gctgccaaca gcgattcacc gtatttacgc gctgcatcgg catttccatg ccaaatcaat
     5581 gaaaacaaat tatggcggtt atactcctta ttattctttg tcggaatgga tatgctctgt
     5641 attttggcca attttctgaa agaaacacca ctatatcccc cccctgattt ttcttcattg
     5701 atggcgtggg atatagcgga atttgtcacg ttaatgatat tacttttcat tcctttattt
     5761 tcattagcag tattgctgcc tattattagg tgcatatttt tgttgtttgc tggatgtaat
     5821 ccatagttat ttattgacat ttttgttcat cctttttgct aaaaaaaaca ataacctatt
     5881 tttgtaatgt aatagatatt agtctattgc caatctaaaa aaaccattgc cacattgaat
     5941 ggctatattc accaataaat tctttcgatt tcgtatattg acatatcgta gatatatctt
     6001 atccctaaga ttcaataatc gagtccgttt accattctgt gcaagcgcct tcactcagaa
     6061 gtgaccgtca aggcgatcac caaacttgat aataaagcag ctcattgcca tacgccagtc
     6121 cttaaggggc gttgtccatt tctgtgatgc tgcctagatt taactgccag ctccggctta
     6181 tctacatcta aatatgtgag cctaataccg ctacactttg gccagactat gtttttctcc
     6241 gggaagtggg ctaaccgtct ccataaagtg acgaactttt ttcaacagtg gccatattga
     6301 acggcactga tgattacggg ttattgtctc atgaagagcc ggccacatca ctgacagcag
     6361 tgacttcagg ccttcagcat cagacagcct gaaccagtta atccatcgcc cgacagattt
     6421 agtaatctag ggctggtttt tcttcagctg ttgcggttcg aaagtgccat tacgatcacg
     6481 cagagtacgg atttccagag gttcatctcc agtagtgata atcttcgtta agcaaccatt
     6541 gcgggaattg ctgccagatc taagctgatt tttctcgcac ccaagatgat gagtcatatt
     6601 agcattgagt acggtttcat tttcagcatc caatcaaact gactgagatc ttcaggattt
     6661 ttgagacttt tggctagttc attagctaaa gcccgcaact tattagatgt caactgtttt
     6721 tcattcataa attcaccttc atttgatatc aaattaaata gttcaacatc agagaattat
     6781 acaaacttat gtacaggctc ctatgatctt cgaatcctga agattatagt gaaaataagt
     6841 tttcacgttg aaatcccaca tagttagtat atgataaata atcatggatg acctgattaa
     6901 cctcttgggg tagtgctttt atagaagaat aaatattcta ctaatttaat acatcaccta
     6961 aaatagtatt gttttttatg gttggttgaa ggtcgtggtg atgatgttag tccttttgag
     7021 aatattccag tgccagatct tacttataat aaaggggctt ggcagtttgg ataaactatt
     7081 gttcttattc cactaataca aactttttgt aaagtatatt aaatatactt catttcatac
     7141 tgaaatcccc ggaagtattc aatcaaacaa ttacaccata aaaggaagag ggggccctaa
     7201 ttaagttgtc aagcatgttt atgatacctg cagggtataa attttctggc tgcaagcaga
     7261 cacatataaa acgtctttat tgatcctacc cacataatat ggacacagtc gtaagtgagg
     7321 ctctggtttt caaattgttc cggactgaga ccaccacacc aacggtgccg tcgccagcga
     7381 ttgtaatcgc actcgatata actaaacact gttgcccgca ctatttcccg atggataata
     7441 gtgttctccg tggagacatt ccattttcag cgaatgaatt ttcatctgtt gctgtatcca
     7501 gtcatacagt gactgcatca gtggcgcggc tctggctttt cttgccgcca gacgctgttc
     7561 tgctgaacat ccccggactt ctgcctcgat ggcatacagt tcaccgatac gctgctgggc
     7621 ttccgttatt ctgccggatt cgtacaacgc ccggtaacta ccgtaagcat cggcctgaag
     7681 cactccgctg taaccggcca gatgattctg tggatggata cctttccggt ccggtgagta
     7741 cgcgaaccag accgccggag gcatttccga accggcgtta cggtcatcac gaacgtagac
     7801 ccacagccgg gcagtccttg ttttaccgct gcccggctcc tggaccggaa cggggatatc
     7861 atcggcatgg actttaccgg gcatcagcac atactggcgc aggatgtcat acagcggttc
     7921 cagcagttca gcaacagcac ctgtccagcg ccccagtgtg gcacggctca gctccacgcc
     7981 ctgacgacgg tatatttctg actggcggta taacggcaga tagtctgcat attttccagc
     8041 gacaacatgg gccagaagcc ccgctccggc ataactgcgt gcaatgggtt ttgaaggcac
     8101 cggtgcctgc acgatatggt cacaccggca acaggccagt ttcggacgtt gtgtttcgat
     8161 aaccttaaag gcgctgctga taagctccag ctgctctaac acatcacatc ccagagaact
     8221 gagttcgcca ccacaggccg ggcagcattc ctcttccgga cggatgaccc gggtttcacg
     8281 gggaagtgag gcaggtaacg gtttatgggc tgaagactgg cgcaggggag atggcagtac
     8341 cgggtcatat tgctcaccca gcgtttccgc catttcttcc tgaagtgcgc tgattcgctc
     8401 ctgtgcttcc tgtatctgcc gttcggtttt tgcgcgaagt ttttctgagc ttttaccgaa
     8461 ctgcatgcgc tgcagtttcg caaccagcgc cttcagccgg ttgatttcgg aagcataagc
     8521 cgccacccgc tgtgagagca ggcggttgta tttcgccatc tggcggatgg tgtcctgttg
     8581 cgtctgcaac agtgcccgca ggcgggcatt ctcatgagca agtgaggtgt ccatatcctc
     8641 actttacaac gggttatatg cggatgccag cgcgttccgt tcgtttcggg tgtttccagt
     8701 cgatgccctc aagaagcatg gataactggg ccggagtaag gtgcaccttg ccgtcacggg
     8761 tgaccggcca gacgaagcgg ccccgctcca ggcgtctggt aaagaggcac agtccgtcac
     8821 tgtcagccca caacactttt atctggtcac cccggcgtcc gcggaagatg aacagatgcc
     8881 cggagaacgg gtcatccttc aggacgttct gaacttttga ggccaggccg ttaaagccat
     8941 ttcgcatatc ggtgatacct gcaaccagcc agatacgcga acctgcaggg aaagatatca
     9001 tcagtggctg ctccctttta tttcgcggat aagtatctgt aataacgccg gagtcagttt
     9061 acctttaagc ctga
//
LOCUS       DAEEBB010000155         9072 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEEBB010000155 DAEEBB010000000
VERSION     DAEEBB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.155"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="KLF07_003564"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="KLF07_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBD9833488.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="KLF07_003565"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="KLF07_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBD9833489.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="KLF07_003566"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="KLF07_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBD9833490.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="KLF07_003567"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="KLF07_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBD9833491.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="KLF07_003568"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="KLF07_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBD9833492.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="KLF07_003569"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="KLF07_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBD9833493.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="KLF07_003570"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="KLF07_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBD9833494.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8343..9040
                     /locus_tag="KLF07_003571"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="KLF07_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAEEBB010000156         8838 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEEBB010000156 DAEEBB010000000
VERSION     DAEEBB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.156"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            812..1054
                     /locus_tag="KLF07_003572"
     CDS             812..1054
                     /locus_tag="KLF07_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833495.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="KLF07_003573"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="KLF07_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBD9833496.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="KLF07_003574"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="KLF07_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD9833497.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="KLF07_003575"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="KLF07_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBD9833498.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="KLF07_003576"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="KLF07_003576"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD9833499.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="KLF07_003577"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="KLF07_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBD9833500.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8838
                     /locus_tag="KLF07_003578"
     CDS             8087..>8838
                     /locus_tag="KLF07_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBD9833501.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2232 c         2265 g         2220 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgcggc
     8701 gattcaggaa attaatgata agcgccagct tgcggaactg aaaaatattg aagaaagaga
     8761 agggaaaaca ttccactact actctctggc agtcatgatt tcagcgaaac aaattaataa
     8821 cctgatttcg caagagaa
//
LOCUS       DAEEBB010000157         8633 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEEBB010000157 DAEEBB010000000
VERSION     DAEEBB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.157"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="KLF07_003579"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="KLF07_003579"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBD9833502.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="KLF07_003580"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="KLF07_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBD9833503.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="KLF07_003581"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="KLF07_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBD9833504.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="KLF07_003582"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="KLF07_003582"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBD9833505.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="KLF07_003583"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="KLF07_003583"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBD9833506.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /locus_tag="KLF07_003584"
     CDS             complement(7825..8136)
                     /locus_tag="KLF07_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD9833507.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="KLF07_003585"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="KLF07_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBD9833508.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAEEBB010000158         8560 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEEBB010000158 DAEEBB010000000
VERSION     DAEEBB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.158"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(270..1043)
                     /locus_tag="KLF07_003586"
     CDS             complement(270..1043)
                     /locus_tag="KLF07_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBD9833509.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="KLF07_003587"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="KLF07_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBD9833510.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="KLF07_003588"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="KLF07_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBD9833511.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="KLF07_003589"
     CDS             1926..2666
                     /locus_tag="KLF07_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBD9833512.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="KLF07_003590"
     CDS             complement(2637..3404)
                     /locus_tag="KLF07_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBD9833513.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="KLF07_003591"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="KLF07_003591"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBD9833514.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="KLF07_003592"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="KLF07_003592"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBD9833515.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="KLF07_003593"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="KLF07_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBD9833516.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="KLF07_003594"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="KLF07_003594"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBD9833517.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8560)
                     /locus_tag="KLF07_003595"
     CDS             complement(8354..>8560)
                     /locus_tag="KLF07_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBD9833518.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2063 a         2148 c         2242 g         2107 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca
//
LOCUS       DAEEBB010000159         8533 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEEBB010000159 DAEEBB010000000
VERSION     DAEEBB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.159"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="KLF07_003596"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="KLF07_003596"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBD9833519.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="KLF07_003597"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="KLF07_003597"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBD9833520.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="KLF07_003598"
     CDS             complement(1322..2986)
                     /locus_tag="KLF07_003598"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBD9833521.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLERLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /gene="mhpR"
                     /locus_tag="KLF07_003599"
                     /pseudo
     CDS             3028..3861
                     /gene="mhpR"
                     /locus_tag="KLF07_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="KLF07_003600"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="KLF07_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBD9833522.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="KLF07_003601"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="KLF07_003601"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBD9833523.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="KLF07_003602"
     CDS             8269..>8533
                     /locus_tag="KLF07_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833524.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2389 c         2334 g         1869 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc tttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAEEBB010000160         8401 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEEBB010000160 DAEEBB010000000
VERSION     DAEEBB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.160"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="KLF07_003603"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="KLF07_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBD9833525.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="KLF07_003604"
     CDS             2089..2238
                     /locus_tag="KLF07_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833526.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="KLF07_003605"
     CDS             complement(3005..3445)
                     /locus_tag="KLF07_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBD9833527.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="KLF07_003606"
     CDS             3663..4052
                     /locus_tag="KLF07_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833528.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="KLF07_003607"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="KLF07_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="KLF07_003608"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="KLF07_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBD9833529.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="KLF07_003609"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="KLF07_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBD9833530.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="KLF07_003610"
     CDS             complement(6238..6480)
                     /locus_tag="KLF07_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBD9833531.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="KLF07_003611"
     CDS             complement(6786..7622)
                     /locus_tag="KLF07_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD9833532.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="KLF07_003612"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="KLF07_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBD9833533.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1889 a         2322 c         2332 g         1858 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aactgacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAEEBB010000161         8360 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEEBB010000161 DAEEBB010000000
VERSION     DAEEBB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.161"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            158..1030
                     /gene="rluB"
                     /locus_tag="KLF07_003613"
     CDS             158..1030
                     /gene="rluB"
                     /locus_tag="KLF07_003613"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBD9833534.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(1070..1660)
                     /gene="cobO"
                     /locus_tag="KLF07_003614"
     CDS             complement(1070..1660)
                     /gene="cobO"
                     /locus_tag="KLF07_003614"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBD9833535.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1657..2415)
                     /locus_tag="KLF07_003615"
     CDS             complement(1657..2415)
                     /locus_tag="KLF07_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBD9833536.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2635..3684
                     /gene="sohB"
                     /locus_tag="KLF07_003616"
     CDS             2635..3684
                     /gene="sohB"
                     /locus_tag="KLF07_003616"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBD9833537.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3720..3971)
                     /locus_tag="KLF07_003617"
     CDS             complement(3720..3971)
                     /locus_tag="KLF07_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBD9833538.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4351..6948
                     /gene="topA"
                     /locus_tag="KLF07_003618"
     CDS             4351..6948
                     /gene="topA"
                     /locus_tag="KLF07_003618"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBD9833539.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            7158..8132
                     /gene="cysB"
                     /locus_tag="KLF07_003619"
     CDS             7158..8132
                     /gene="cysB"
                     /locus_tag="KLF07_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBD9833540.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         2141 a         1990 c         2190 g         2039 t
ORIGIN      
        1 cgcctcaaag ccaggccagt taaaacgtcg agtcgtttac ttaaggcctg aagagttcaa
       61 acagacttta ctatcagtga gtcagatgta agttctgact cactgttgca caaaagcata
      121 tcagacgcct gggaaggcga caccaaaagg aagctctatg agcgaaaaat tacagaaagt
      181 gctggcccgc gctggccacg gctcccgccg tgaaatcgaa tctattattg aagccggtcg
      241 tgtgagtgtt gatggcaaaa ttgctaaact cggcgatcgc gttgaagtta cccctgggct
      301 gaaaattcgt atcgatggtc atctgatttc ggtacgtgag tccgctgaac aaatttgtcg
      361 cgttctggcc tattacaaac cggaaggtga gttgtgtacc cgtaacgatc cggaagggcg
      421 tccgacggtg tttgaccgtc tgccaaagct gcgtggcgca cgctggattg ccgtaggtcg
      481 tctggacgtt aacacctgtg gtctgctgct gttcaccacc gatggtgaac tggcaaaccg
      541 tttaatgcat ccaagccgtg aagttgaacg tgaatacgcc gtgcgtgtat ttggtcaggt
      601 tgacgacgcg aaactgcgtg atttgagtcg tggcgtgcaa ctggaagatg gtccggcagc
      661 ttttaaaacc atcaagttca gcggcggcga agggatcaac cagtggtaca acgtgactct
      721 gaccgaaggc cgtaaccgtg aagttcgtcg tctgtgggaa gcggttggtg tgcaggtgag
      781 ccgcctgatc cgtgttcgtt acggtgatat cccactgcca aaaggtctgc cacgcggtgg
      841 ctggacggag ctggacctcg cccagactaa ctatctgcgc gaactggtgg agcttccgcc
      901 agaaaccagc tctaaagtcg ctgtagaaaa agaccgtcgt cgcatgaagg ctaatcagat
      961 tcgtcgtgcg gtgaaacgtc acagtcaggt gagcggcggt cgtcgttctg gcgggcgtaa
     1021 taacggttaa tcaaagtatg ccggacgtca tatccggcat ttttacagat taataatcaa
     1081 tccctatttg cgccttaaca ccggcatcaa acgcatgttt gatggggcgc agctcactta
     1141 ccgtatccgc cagttcaaga atatcccgat gacagcctcg acccgtgata atcaccgtct
     1201 gttgatgtgg acgttcattt aacgcctgca ccacttcttc cagtggcaaa tagtcatacg
     1261 ccaccatata cgtcagttca tcaagcaaaa ccatatccag tgaggaatca gcaagcatcc
     1321 gctttgcatg ttgccagact tcgcggcagg cggcggtatc agactcgcgg ttttgtgtat
     1381 cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc atgtggctcc agcagattgc
     1441 gttcgccatt aggccaggtg cctttaataa actgcacgac gcccactttt tttccgtgac
     1501 caactgcgcg tgttgccgta ccaaatgccg cggtggtttt gccttttcca ttgccggtaa
     1561 agacgatgat aataccgcgt tcatcctggg cctgggccac acgagcatct actttttctt
     1621 tcactcgctg ctgacgctgt tggtagcgtt catcactcat tgggaaattc ctggtttacg
     1681 gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg ctgtcatcgc ccatcagcca
     1741 gaggtagagc ggcatgatat cagcgggtgt tttaagtttc tgtggatctt cggtcgggaa
     1801 ggcgcttgcg cgcattgcgg tgcgcgtacc gcctgggtta atgcagttga cgcgcaggcg
     1861 ctgctgatat tcatcggcca gtacctgcat catcccttcg gtggcaaatt tcgacgctgc
     1921 atatgcaccc cagttggctc gtccctgacg cccaacgctt gatgaagtaa agaccagtga
     1981 accggcgtcc gatttgagta ataaaggaag cagtgcctgg gtgagcataa aggtggcatt
     2041 aacgttgacc tgcatgacgt cctgccagac ctgcggattt tgttcgctca ttgggcaaac
     2101 atcgccgagc aatccggcat tatgcaaaac accatccaga cgcggataat taacgacaat
     2161 gcgctgtgcc agttgttggc aattttcgga cgtgcaggtc agcaaatcga gaataaacca
     2221 ctgtggctga cgcccagttt cttcgtttat gtggctggct acctgacgta atttttcttc
     2281 attacggccc aacagaatca ctgtcgcacc atagcgtgca tacgtcatcg cggcttcacg
     2341 accaatccca tcgctggctc ccgtcaccag gataatgcga tcattgagta aatcttgttt
     2401 tggctggtaa tgcatggcta ctcctcaacg acgttgtctg tcgcgctgcc agtgtacttt
     2461 atgactttgg ggctttatgc ctgaaacaga agggcatttc aatcagcctg gggcgataac
     2521 accgcaaaat ggatactttg tcatactttc gctgcaataa catctctgcg agacggctta
     2581 acatgcctgt tgtaaactgt gagccaaagc gttgtttaac caaggtgggg actcgtggaa
     2641 ttgttgtctg aatatggttt gtttttggcg aaaatcgtta ccgttgtgct agcgattgcg
     2701 gcgattgccg ccattattgt caatgttgct caacgtaata aacgccagcg tggcgagtta
     2761 cgggtcaaca atctcagcga acagtataag gagatgaaag aagaactggc cgcggcgctg
     2821 atggactcac atcagcaaaa acagtggcac aaagcgcaga agaaaaagca caagcaagaa
     2881 gcgaaagcag caaaagcgaa agccaaactg ggcgaggtgg caactgacag taaacctcgc
     2941 gtctgggtgc tggattttaa aggcagcatg gacgcccatg aagtgaactc gctacgtgaa
     3001 gagataacgg cggtactcgc agcattcaaa ccgcaggatc aggttgtgct ccgtctggaa
     3061 agccctggtg gcatggtgca tggttacggg ttggcggctt cgcagctgca gcgtctgcgt
     3121 gataaaaaca ttcctttaac tgttacggta gacaaagtcg ctgccagcgg cggttacatg
     3181 atggcctgtg tggcggacaa aattgtttcc gcaccgtttg ctattgtggg ttccattggg
     3241 gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa gcaaagatat tgatatcgaa
     3301 ctgcacaccg ccgggcagta taagcgcacg ctgaccttgc tgggtgaaaa taccgaagaa
     3361 gggcgggaga aattccgcga agagctgaac gaaacgcatc agttatttaa agattttgtg
     3421 aagcgtatgc gtccgtctct ggatattgaa caggtggcaa cgggtgaaca ctggtacgga
     3481 caacaggcgg tagagaaagg cctggttgat gaaatcaaca ccagtgatga agttattctt
     3541 agcctgatgg aaggccgtga agtggttaat gtacgctata tgcagcgtaa acgactcatt
     3601 gaccgattca ccggcagcgc ggcagagagc gccgatcgat tgttgctacg ctggtggcag
     3661 cggggtcaaa agccattgat gtaaaagaca aacgcgaggc taagacctcg cgttttgctt
     3721 taatcaacca gatgatattt ttctgaaagc acatgggcca ggtgtttgaa catattaaac
     3781 accgcggtgc ttttggctgt tggcaatcct tgttcatcta aaaagtagtc gccgataaat
     3841 accagcacgc cattacgctg cgtgacgccg gtggcttcaa tccctgcgag cgtatcctca
     3901 tgctcacgaa tgattttgtt ggcctctttc aacagcgttt cgcggtcgat gggttgtgtc
     3961 tctttgttca ttatttactc cttaaacaag gacattagtc tacgccaggc atggcttgca
     4021 gacaaatata ccacgctggt ggcaagagcg ccttactggc aactttggat tttgcatgct
     4081 aataaagttg cgtatcggat tttatcaggt acagtgtgac gctttcgtca atctggcaat
     4141 agatttgctt gacattcgac caaaattccg tcgtgctata gcgcctgtag gccaagacct
     4201 gttaactcag tcacctgaat tttcgtgaac agagtcacga caaggggttg atatccgcag
     4261 agaacgagtc catatcggta actcgttgcc agtggaaggt ttatcaacgt gcgacgcatt
     4321 cctggaagaa tcaaattagg taaggtgaat atgggtaaag ctcttgtcat cgttgagtcc
     4381 ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg actacgtggt gaaatccagc
     4441 gtcggtcaca tccgcgattt gccgaccagt ggctcagctg ccaaaaagag tgccgactct
     4501 acctccacca agacggctaa aaagcctaaa aaggatgaac gtggcgctct cgtcaaccgt
     4561 atgggggttg acccgtggca caattgggag gcgcactatg aagtgttgcc tggtaaagag
     4621 aaggtcgtct ctgaactgaa acaactggct gaaaaagccg accacatcta tctcgcaacc
     4681 gaccttgacc gcgaagggga agccattgca tggcacctgc gggaagtgat tgggggtgat
     4741 gatgcgcgct atagccgagt ggtgtttaac gaaattacta aaaacgcgat ccgccaggca
     4801 tttaacaaac cgggtgagct gaatattgat cgtgttaatg cccagcaggc gcgtcgcttt
     4861 atggaccgcg tggtggggta tatggtttcg ccgctgctat ggaaaaagat cgctcgtggt
     4921 ctgtctgccg gtcgtgtgca gtcggtggcg gttcgcctgg tggtcgagcg tgagcgtgaa
     4981 attaaagcgt tcgtgccgga agagttctgg gaagtcgatg ccagcacgac cacgccatct
     5041 ggtgaagcgt tggcgttaca ggtgactcat cagaacgaca aaccgttccg tccggtcaac
     5101 aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag cgcgctacag cgtgctggaa
     5161 cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt ttattacctc tacgctgcaa
     5221 caagctgcca gcacccgtct tggatttggc gtgaaaaaaa ccatgatgat ggcgcagcgt
     5281 ttgtatgaag caggctatat cacttacatg cgtaccgact ccactaacct gagtcaggac
     5341 gcggtaaata tggttcgtgg ttatatcagc gataattttg gtaagaaata tctgccggaa
     5401 agtccgaatc agtacgccag caaagaaaac tcacaggaag cgcacgaagc gattcgtcct
     5461 tctgacgtca atgtgatggc ggaatcgctg aaggatatgg aagcagatgc gcagaaactg
     5521 taccagttaa tctggcgtca gttcgtagcc tgccagatga ccccagcgaa atatgactcc
     5581 acgacgctga ccattggtgc gggcgatttc cgcctgaaag cacgcggtcg tattttgcgt
     5641 tttgatggct ggacaaaagt gatgcctgcg ttgcgtaaag gcgatgaaga tcgcatctta
     5701 ccagcagtta ataaaggcga tgctctgacg ctcgttgaac ttacaccagc ccagcacttt
     5761 accaagccgc cagcccgttt cagtgaagca tcgctggtta aagagctgga aaaacgcggt
     5821 atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca ttcaggatcg tggctacgtg
     5881 cgagtagaaa atcgtcgttt ctatgcggaa aaaattggcg aaatcgtcac cgatcgcctt
     5941 gaagaaaatt tccgcgagtt aatgaactac gacttcaccg cacagatgga aaacagcctc
     6001 gacctggtgg caaatcacga agcagagtgg aaagctgtac tggatcactt cttctcggat
     6061 ttcacccagc agttagataa agctgaaaaa gatccggaag agggtggtat gcgcccgaac
     6121 cagatggttc tgaccagcat tgactgcccg acttgtggtc gcaaaatggg gattcgcaca
     6181 gcgagcaccg gggtattcct tggctgttct ggttatgcgc tgccgccgaa agagcgttgc
     6241 aaaaccacca ttaacctggt gccggaaaac gaagtgctga acgtactgga aggcgaagac
     6301 gctgaaacca acgcgctgcg cgcaaaacgt cgttgcccga aatgcggcac ggcgatggac
     6361 agctatctca tcgatccgaa acgtaagttg catgtctgtg gtaataaccc aacctgcgac
     6421 ggttacgaga tcgaagaggg cgaattccgc attaaaggtt atgacggccc gatcgttgag
     6481 tgtgaaaaat gtggctctga aatgcacctg aaaatggggc gtttcggtaa atacatggcc
     6541 tgcaccaacg aagagtgtaa aaacacacgt aagattttac gtaacggcga agtggcacca
     6601 ccgaaagaag atccggtgcc attacctgag ctgccgtgcg aaaaatcaga tgcttatttc
     6661 gtgctgcgtg acggcgctgc gggtgttttc ctggctgcca acactttccc gaaatcgcgt
     6721 gaaacgcgtg cgccactggt ggaagagctt tatcgcttcc gcgaccgtct gccggaaaaa
     6781 ctgcgttatc tggccgatgc gccacagcag gatccggaag gtaataagac catggttcgc
     6841 tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa aagacggaaa ggcgactggc
     6901 tggtcagcat tttatgttga tggcaaatgg gttgaaggaa aaaaataacc tttaattctg
     6961 tcaggttttt ataaacaaag ggtcgcgaaa gcggcccttt tttattgcat attatttttt
     7021 cttcacacct atacactaag gctataaatg atatagtggt tatagttagc acctttttta
     7081 ttattaaatc gtattagtca cccgccaggt gtgacgaaaa aacgatgttc tgatggcgtc
     7141 taagtggatg gtttaacatg aaattacaac aacttcgcta tattgttgag gtggtcaatc
     7201 ataacctgaa tgtctcatca acagcggaag gactttacac atcacaaccc gggatcagta
     7261 aacaagtcag aatgctggaa gacgagctag gcattcaaat tttttcccga agcggcaagc
     7321 acctgacgca ggtaacgcca gcagggcaag aaataattcg tatcgctcgc gaagtcctgt
     7381 cgaaagtcga tgccataaaa tcggttgccg gagagcacac ctggccggat aaaggctcac
     7441 tgtatatcgc caccacgcat acccaggcac gctacgcgtt accaaacgtt atcaaaggct
     7501 ttattgagcg ttatccccgc gtttctttgc atatgcacca gggctcgccg acacaaattg
     7561 ctgatgccgt ctctaaaggc aatgctgatt tcactatcgc cacagaagcg ctgcatctgt
     7621 atgaagattt agtgatgtta ccgtgctacc actggaatcg ggctattgta gtcactccgg
     7681 atcacccgct ggcaggcaaa aaagccatta ccattgaaga actggcgcaa tatccgttgg
     7741 tgacatatac cttcggcttt accggacgtt cagaactgga tactgccttt aaccgcgcag
     7801 ggttaacgcc gcgtatcgtc ttcacggcaa cggatgctga cgtcattaaa acttacgtcc
     7861 ggttagggct gggggtaggg gtcattgcca gcatggcggt ggatccggtc gccgatcccg
     7921 accttgtgcg tgttgatgct cacgatatct tcagccacag tacaaccaaa attggttttc
     7981 gccgtagtac tttcttgcgc agttatatgt atgatttcat tcagcgtttt gcaccgcatt
     8041 taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc taatgaagaa attgaggtca
     8101 tgtttaaaga tataaaactg ccggaaaaat aatttcttgc gttattttcg gcacctttta
     8161 tgtagcgaag gtgccggaat atattctctt ttgttactta tttctatacc gtgcacaata
     8221 tttttatccc tcatatttat agggtaaatt acctgtaaag tagtgtctaa ttacccgcct
     8281 cgcaaactcc agttttcaaa tatctatttc aggtcaaaag attgaattat tcactggaga
     8341 cgattcgtaa attcgctgga
//
LOCUS       DAEEBB010000162         8273 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEEBB010000162 DAEEBB010000000
VERSION     DAEEBB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.162"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            52..936
                     /locus_tag="KLF07_003620"
     CDS             52..936
                     /locus_tag="KLF07_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBD9833541.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="KLF07_003621"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="KLF07_003621"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBD9833542.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="KLF07_003622"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="KLF07_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBD9833543.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="KLF07_003623"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="KLF07_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBD9833544.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="KLF07_003624"
     CDS             5160..6443
                     /locus_tag="KLF07_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBD9833545.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="KLF07_003625"
     CDS             6590..8065
                     /locus_tag="KLF07_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD9833546.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1915 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actgaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cgg
//
LOCUS       DAEEBB010000163         8217 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEEBB010000163 DAEEBB010000000
VERSION     DAEEBB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.163"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="KLF07_003626"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="KLF07_003626"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBD9833547.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="KLF07_003627"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="KLF07_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBD9833548.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="KLF07_003628"
     CDS             2031..2549
                     /locus_tag="KLF07_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBD9833549.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="KLF07_003629"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="KLF07_003629"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBD9833550.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="KLF07_003630"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="KLF07_003630"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBD9833551.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /gene="ydfH"
                     /locus_tag="KLF07_003631"
     CDS             5611..6297
                     /gene="ydfH"
                     /locus_tag="KLF07_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBD9833552.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="KLF07_003632"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="KLF07_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBD9833553.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="KLF07_003633"
     CDS             complement(6713..8173)
                     /locus_tag="KLF07_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD9833554.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2173 a         2055 c         1871 g         2118 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt ccttaaatat aaatatggca aactatatgt tttgtta
//
LOCUS       DAEEBB010000164         8184 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEEBB010000164 DAEEBB010000000
VERSION     DAEEBB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.164"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..400)
                     /locus_tag="KLF07_003634"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="KLF07_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(586..3894)
                     /gene="icsA"
                     /locus_tag="KLF07_003635"
                     /gene_synonym="virG"
     CDS             complement(586..3894)
                     /gene="icsA"
                     /locus_tag="KLF07_003635"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBD9833555.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVVGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4423..5625
                     /gene="virA"
                     /locus_tag="KLF07_003636"
     CDS             4423..5625
                     /gene="virA"
                     /locus_tag="KLF07_003636"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBD9833556.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASNSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5804..6491
                     /locus_tag="KLF07_003637"
                     /pseudo
     CDS             5804..6491
                     /locus_tag="KLF07_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6084..6200
                     /locus_tag="KLF07_003637"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6493..6859
                     /locus_tag="KLF07_003638"
                     /pseudo
     CDS             6493..6859
                     /locus_tag="KLF07_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6787..7260
                     /locus_tag="KLF07_003639"
                     /pseudo
     CDS             6787..7260
                     /locus_tag="KLF07_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            8013..>8184
                     /locus_tag="KLF07_003640"
     CDS             8013..>8184
                     /locus_tag="KLF07_003640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833557.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNI"
BASE COUNT         2405 a         1876 c         1634 g         2269 t
ORIGIN      
        1 gttcagttca ggctgtacgc ttccccatgt aatttgcctc gccacttcac cgggctcagg
       61 accccgtagg atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg
      121 tagacctgtt actaatttat acctactctg accaagtctg gataagaaag gcacacctgc
      181 acatttctac ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata
      241 ttcctcgtac agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt
      301 catatattac tcgaccttgt tgagatacaa caacacgtgc attagtttct gcaataccag
      361 taatctgagg cgcataacca cgaatatttg gcggtagcat tcgttcatcg ctttctaatg
      421 caattcctgt gtaacgccaa gcaacagcgc attctggcgc atgatctggt aaacacgttt
      481 ggcattgatc gcaggcatac catcatgtgc acaaaaaccc accgagcttt tacacggtgg
      541 gtcccagaga aatgcaggac atcaacacgc cctgcatttt tattatcaga aggtatattt
      601 cacacccaaa ataccttggg tgtctctgta actgttatta cccaattgct gagatacgtt
      661 tccccagatg cttaggttat ctgcaagacg cccccccaca ccagttttga gttcaatcac
      721 attacggttg cctatctggt gatttgacat gccattcata attacaccat attgctttga
      781 gctccatttc caattcactt ccaaaaatgg ttcgaactta cgaagagtct ccttatcgat
      841 acaactctga ctaattaagg atgtacgcaa tcccaatttt gcttgaatat tattgacccc
      901 agaggatgtg accatagtgc cattgtgttc aatatagtcg tttgctttta ccccatgcct
      961 agttatctgg acttgtggat tgagatacaa tgcgttgtca atattattat gagcagtcca
     1021 gcgtatggtt gggtagatat agccagcttc caaagctcct gttattcctt tgctggaata
     1081 tttctcacca gttaacccat ctcctttgac tgtattatta aaccaaccgt actgcaacca
     1141 agcatccata tatagacctg ttcgttcttt ttcattctga aaccaagaac tgtacaaacc
     1201 ggcagtataa ccgctcacag ttcctcgtga actataacta gtatacttat tatgagtttt
     1261 gctatcctga tatccataag ctcccataat acccacatgc aagccatcat gatcagtgaa
     1321 gtttgtcttc aaaatatctc caccaatctg ataaaccatc ctgttagctg tggtttttaa
     1381 ttgtctatca gctaaattat gctcattatg tccaccaaca gtacgaatcc acatggtggt
     1441 ttcgctgagc tgacctgtag taggatcaac aatagtgcta ccgtgtcggt catagagaga
     1501 aggactaaac atcgtgttca ttgctatcag gttagcaata taactgccag cttcagggcg
     1561 ccatgtgtga atactacctt cacccaaatt aagtgaagat acaagctgtg aactagctcg
     1621 gcgactactc atttgagtag actcttgatt gctcatctgt tttgattctt gattatccat
     1681 ctgactagtt agataccact tattggtatt cagtccagat acagtgccct gtttcaggcg
     1741 atagtcataa ctaccagcaa caatacggcc tttctgaata aaagcattct tatcagaaga
     1801 atctgtcgaa ataatttgaa caccttcaag agttttatct ccagagcctc cctcatttgt
     1861 cactgcaaca taggtaatac cagacgcatg gcctgcaatt tccagccggt cagttttact
     1921 gttgtcattg ccgagaaccg cgcccaaaga taatattccg ccctttccat gccaattacc
     1981 tttctgaaca taggtctgac cgacatttga cgaactatta ttgagaataa catgaccgtt
     2041 tttctccaga gtcatgttac ctgtgacaat gacggggtca ggcaaaaaag gggcattaat
     2101 attattgatt agcacagtcc cactatttaa taaagtgtta acagtttggt tcatgcctga
     2161 aaaattcaag gtagcacctt tattaacaat aacaccagcg gtacgtgtca tagcttcaac
     2221 tgtccccatt tttagaatgc ctgcattgat gttggtgaaa ccagagtagg tatttttttc
     2281 cgccaaaatg agagttccct cccctgattt tgttagggat tttccgtccc agctatttat
     2341 attatgatgg ctgagattat ctgccagaat agtactaaca gtaagttcag cgttttcttt
     2401 cagattgaac tctccatgac tgtcgccatc tgtatcattc cagctcaaac cgtagatcaa
     2461 tttattctgg tcactgatat agctttttgt atagtcaata gcaccagtag tgagtaacgg
     2521 attgatgata gatatattag aaacactgaa acctgttatc cctttatctg cacttagcag
     2581 tttatagtcc ttctgcgccc agttatttat aatagcttcc acttttacat tgttgccaaa
     2641 tgacatattt ccagaagctt ccagactact gattccagct aaatttaaag aactgttctc
     2701 accaaaagaa acgtcacctg aaacagtgaa tttgttacct gatagtgaaa cagttgtaca
     2761 atcaccagca cataaatcac cttcaatagt tggtgtggtc ccagtgtaat cattgttgat
     2821 aattttcaga atggatgaat tgttaacctg tacatcacca gtaatgacag aaccttcatg
     2881 gagtattaat gaattgtttc caccagtgat gtggatagca ttacctacta cattttgtgt
     2941 tccatcatct tctttacctg aaattatata tccgctatta gcaatggtca tattgctacc
     3001 atttatagca tccccccaat gtccaccatt accgcctaaa atagctccat tgttgattat
     3061 agacaggtta tctcctgtga tagcattacc accgtagcca tcataaccat aaccgtaagc
     3121 agcacctccg ttgcctccag cgaacgtgcc cttattgatt acagagaggc tgcttcctgt
     3181 gatggcatca cctccattac cgccatcacc ctcaccataa ttgttacctc cattaccacc
     3241 ggaaatagtc cctttgttga ttatgggtag attgactcct gtgacggcat cacctccatt
     3301 ccccccatca ctatcactat aactatcgcc accatgtcca cctgaaataa tttctccatt
     3361 tattataaac agattgtctc ctgtaacagc ctcaccacca tcaccgttat ggtcagcacc
     3421 gctaccgccg ttaccaccaa gaatcatgcc ttgattgatt atagacaagt cagatccggt
     3481 aatagagtca ccaccattac cgccacaact attaccatca ttattatcac cagcaccacc
     3541 atgacctgcg ctaatcataa tatcagagtt aatccgtagc ttagttctag atgcatgaga
     3601 ggggttggaa gaagttatat accacgcatc cattccatct tcaccagctc ccaagggaga
     3661 aagtccatca acaggtgtta ataatttttc ataattgtcc tctgaaaaat gaagttcttg
     3721 agtacccgaa agaggaatag caaaagctat tggccccccg agcaacaggg atgcaccaac
     3781 aacaaaagga gaaaaagaca atttgcatgt tttttccttt accgtaggta attctccggc
     3841 ccccccctgt gaacattggg tcatattaca aaaaaatttg tgaatttgat tcatgcacta
     3901 tattatcagt aagtggttga taaaccccta aaaagattcc gttttatccg gaataaaggg
     3961 acgatatatg caaaaatata ttaaacaaag cctcaaccaa aacatgagag ctgtgttata
     4021 tatcaatgca acgggagaaa tttacctgtg tcttgctttg aaaaaaagag ggggaaagct
     4081 atatgttcga taataaatta ataattttac tatgatagtc tgcttcagac cgcaactttt
     4141 cagttctttc tgtacgcttg cccaaagttt aaccactttg tttttctata cacccaatgg
     4201 ttctatctgc cccttgatgt taatccccaa agccaataac atgaatgtat tcatatcaca
     4261 actttcctac cagactctcc aatgcaataa ctgcataatt gtttttagtg gaaatgtatc
     4321 gttttcttaa agagaagaat aacattccat tttattatgt tcccaattta attatattaa
     4381 tatatattgg cattaatagg aaaatacatc aggagaaatc aaatgcagac atcaaacata
     4441 actaaccatg aaagaaatga ctcatcatgg atgtcaactg tcaaatcaac tacagaagta
     4501 agttggaata aattgagttt ttgtgacgtt ttactgaaaa taataacttt cggcatatat
     4561 tctccacacg aaactctagc cgaaaaatat agcgaaaaaa aactaatgga tagtttctca
     4621 ccatcactat cacaagataa aatggacgga gagtttgccc acgcaaacat agatggcatt
     4681 tcaatacggc tatgccttaa caaaggcata tgttcggttt tttatcttga tggagataaa
     4741 atacaatcca ctcaactttc atcaaaagaa tataataact tgttaagttc actccccccc
     4801 aaacaattca acttaggaaa agtacatact attacagctc cggtatcagg gaattttaaa
     4861 actcacaagc ctgcaccaga agttattgaa acagccatta attgctgtac atcgataata
     4921 ccaaacgatg attacttccc cgttaaagac actgatttta atagtgtttg gcatgatatt
     4981 tacagagata ttagagcatc taacagcaat agtacaaaaa tatatttcaa caatattgaa
     5041 ataccactaa aattaattgc agatctaatt aatgagttag gaattaatga attcattgat
     5101 agcaaaaaag agcttcaaat gctaagttac aaccaagtaa ataaaataat caattcaaac
     5161 ttcccccaac aagacctatg ttttcaaaca gaaaaacttt tgtttacttc actctttcaa
     5221 gaccctgcat ttatatctgc actaacatct gcattctggc aatctcttca catcacgtct
     5281 tcctctgtgg aacacatata tgcccaaatt atgtccgaaa acatagaaaa caggcttaat
     5341 ttcatgcctg aacaacgagt tattaacaat tgtggacata ttataaaaat caacgcagtt
     5401 gtccctaaaa acgacactgc aatctctgcc tctgggggga gggcttacga agttagctca
     5461 tcaatattac catcgcacat aacatgtaat ggtgttggta ttaataaaat agaaacaagc
     5521 tatctcgttc atgctggaac acttccaagc agtgaagggc ttcgcaatgc tatccctcca
     5581 gaaagtcgtc aagtatcatt tgccatcata tctcctgatg tttaagccaa tattatattg
     5641 gctgccagac tgtaaaaaaa cgttcttcag aacagctcta taaactatta aaccaaagct
     5701 aaaaggatgg ggaccaatag aatctcagcc catccttttt atatcattat gaaccgcccc
     5761 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatacacg
     5821 tttttcccct gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata
     5881 tgactcacaa tgggcagcga tttgttccat tgccccaaag attggctgta tactggagac
     5941 tctgcgtgtc tgggtacgcc agcatgagcg ggataccggg ggcggtgagg tgggctcacc
     6001 accgctgaac gtcagcgtct gaaagagccg gaacgtgaaa atcgtgaact gcgccgcagt
     6061 aacaatatcc ttcgcctggc ttccgcttat tttgcaaagg cggagttcga ccgcctctgg
     6121 aaaaaataat gccactgctg gataagctgc gtgagcagta cggggtcgga tcggtatgca
     6181 gtgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga catcatcatg
     6241 ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa gaaagagata ctgcgcgtat
     6301 acgatgagaa tcatcaggta tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag
     6361 gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatgggg cttgccggtg
     6421 ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc gcaggcgacc
     6481 gcgtaaaccg tcatcaaggc aatatgccgc gtactccggg tggcccgcag cggctggtat
     6541 acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca gcactgcgac
     6601 agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc ctgacggatg
     6661 aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca
     6721 ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg cacacggcct
     6781 gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga accagaagtg
     6841 ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg gggcgccggg
     6901 ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg ctgatgaaag
     6961 ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa acgccgtgtt
     7021 gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt gatctcgccg
     7081 ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg ctacctgagt
     7141 ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg ccgaagtact
     7201 gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta tgccgatgac
     7261 tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg cggcacaaga
     7321 gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg caccaaacca tccactgctg
     7381 ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg agccgatcag
     7441 gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat
     7501 gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc
     7561 ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc
     7621 gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca atgcggggta
     7681 cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa ttggggcccc
     7741 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     7801 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagtttat
     7861 ccaaactgcc aagccccttt attataagta agatctggta ctaaataagc gttctaaacc
     7921 gccccgagta ggcggcctga cggctgcggc ttgtcatggc ctgtgattac cgttataaaa
     7981 acagacaata tcattgtctt tcaggtagtt atatggcccg ttcagctaaa ccacgcaaac
     8041 gaaaacctgc acctcaaaga agcaaactcc tccgctatgt cgtaaaactt cacgaagatg
     8101 atttttttga cgaagaagaa gctgaggttc tgcgctttga taattttgac gatgccgttg
     8161 agtgttgcgc agacctgaat attc
//
LOCUS       DAEEBB010000165         8182 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEEBB010000165 DAEEBB010000000
VERSION     DAEEBB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.165"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="KLF07_003641"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="KLF07_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBD9833558.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="KLF07_003642"
     CDS             complement(2975..3697)
                     /locus_tag="KLF07_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBD9833559.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="KLF07_003643"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="KLF07_003643"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBD9833560.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="KLF07_003644"
     CDS             6679..>8182
                     /locus_tag="KLF07_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBD9833561.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2029 c         2321 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAEEBB010000166         8155 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEEBB010000166 DAEEBB010000000
VERSION     DAEEBB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.166"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..449)
                     /locus_tag="KLF07_003645"
     CDS             complement(<1..449)
                     /locus_tag="KLF07_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBD9833562.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="KLF07_003646"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="KLF07_003646"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBD9833563.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="KLF07_003647"
     CDS             complement(1788..2507)
                     /locus_tag="KLF07_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBD9833564.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="KLF07_003648"
     CDS             complement(2691..3017)
                     /locus_tag="KLF07_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBD9833565.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="KLF07_003649"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="KLF07_003649"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBD9833566.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="KLF07_003650"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="KLF07_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBD9833567.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="KLF07_003651"
     CDS             4977..5252
                     /locus_tag="KLF07_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBD9833568.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="KLF07_003652"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="KLF07_003652"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBD9833569.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="KLF07_003653"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="KLF07_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBD9833570.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8155)
                     /locus_tag="KLF07_003654"
     CDS             complement(7903..>8155)
                     /locus_tag="KLF07_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBD9833571.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2159 c         1986 g         2087 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc catatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcga
//
LOCUS       DAEEBB010000167         8123 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEEBB010000167 DAEEBB010000000
VERSION     DAEEBB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.167"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            163..317
                     /locus_tag="KLF07_003655"
                     /pseudo
     CDS             163..317
                     /locus_tag="KLF07_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="KLF07_003656"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="KLF07_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBD9833572.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="KLF07_003657"
     CDS             858..2264
                     /locus_tag="KLF07_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBD9833573.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="KLF07_003658"
     CDS             2509..3654
                     /locus_tag="KLF07_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD9833574.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="KLF07_003659"
     CDS             3672..4685
                     /locus_tag="KLF07_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD9833575.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="KLF07_003660"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="KLF07_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="KLF07_003661"
     CDS             5576..6370
                     /locus_tag="KLF07_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9833576.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="KLF07_003662"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="KLF07_003662"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBD9833577.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8026..>8123
                     /locus_tag="KLF07_003663"
     CDS             8026..>8123
                     /locus_tag="KLF07_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HBD9833578.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1919 a         1908 c         2222 g         2074 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgtcacag ttgattagca taaggcttta
     7981 gagtatcgtt attaaactaa taatttataa gtagaagtat taacaatgaa agttcaattg
     8041 tacaaattca cggaagataa aaataaaacc cttaccttca gatggacgaa aaaacacttt
     8101 gagttttgta tggataataa aat
//
LOCUS       DAEEBB010000168         8114 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEEBB010000168 DAEEBB010000000
VERSION     DAEEBB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.168"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..613
                     /locus_tag="KLF07_003664"
                     /pseudo
     CDS             1..613
                     /locus_tag="KLF07_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1227..1603)
                     /gene="rnpB"
                     /locus_tag="KLF07_003665"
     ncRNA           complement(1227..1603)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KLF07_003665"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1636..2781)
                     /gene="garK"
                     /locus_tag="KLF07_003666"
     CDS             complement(1636..2781)
                     /gene="garK"
                     /locus_tag="KLF07_003666"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBD9833579.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2878..3768)
                     /gene="garR"
                     /locus_tag="KLF07_003667"
     CDS             complement(2878..3768)
                     /gene="garR"
                     /locus_tag="KLF07_003667"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBD9833580.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3798..4568)
                     /gene="garL"
                     /locus_tag="KLF07_003668"
     CDS             complement(3798..4568)
                     /gene="garL"
                     /locus_tag="KLF07_003668"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBD9833581.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4584..5918)
                     /gene="garP"
                     /locus_tag="KLF07_003669"
     CDS             complement(4584..5918)
                     /gene="garP"
                     /locus_tag="KLF07_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBD9833582.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6293..7864
                     /gene="garD"
                     /locus_tag="KLF07_003670"
     CDS             6293..7864
                     /gene="garD"
                     /locus_tag="KLF07_003670"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBD9833583.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8013..>8114
                     /gene="prlF"
                     /locus_tag="KLF07_003671"
     CDS             8013..>8114
                     /gene="prlF"
                     /locus_tag="KLF07_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBD9833584.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         2121 a         2130 c         1913 g         1950 t
ORIGIN      
        1 atgttccagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
       61 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
      121 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
      181 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
      241 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
      301 ttaccagaga tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat
      361 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac
      421 tatagattta aacatagaag actctagctc agaggaattc ctttttgata atagcctcga
      481 gaaagacgaa aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc
      541 atataactca gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt
      601 aaatgcaata tagacataat aacttcttac aaaatctata attaataccg tagatattca
      661 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      721 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt
      781 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag
      841 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      901 cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc
      961 caaaagcaaa aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag
     1021 ccgctttctt ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc
     1081 aaaaacccgc cgaagcggtt ttttacgtaa accaggtgaa actgaccgat aagccgcttt
     1141 cttttgggta tagcgtcgtg gacagtcatt catctttccg ctcctccaaa agcaaaaacc
     1201 cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgg gttctgtcgt
     1261 ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta cccgggttca
     1321 gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt ttaccgtgcc
     1381 acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc
     1441 cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt ttccccccca
     1501 ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctccg cccgtctccc
     1561 ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc agtatagagg
     1621 gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt
     1681 acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca acgtacctat
     1741 gctggtcaat acgctgaaga ccgcatcgat gccatgctga tgtacaacgc caacatcatc
     1801 ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca cgtttgcaac
     1861 gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac
     1921 cagcgtacaa tcgtgaatat gttcttccag attcagcgcc gtagtgacga tttcaatacc
     1981 acttttcagt tccgcaccaa gaaacgccat cagcgccgcg cccataccgc ccgcagctcc
     2041 tgcgccgggg acatctttca catcaacatg cagcgctttt ttaatgacat cggcatagtg
     2101 agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa
     2161 gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat
     2221 gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag
     2281 actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc
     2341 ctgtaccatg cctgcgccgc catcatttgt agcgctgccg ccaatgccga taataatgtt
     2401 tgttgcactg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggttac
     2461 gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat
     2521 aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg
     2581 ccctgtaacc caggcgtgac gttcagcccc ctgggtggct gcaatcatcg cttccaccgt
     2641 tccttcacca ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa aaatttcccg
     2701 aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc
     2761 tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct
     2821 cttattcggc ctagattcca ggcccggatt gcaatacacc atccgggcac gacgtcatta
     2881 acgagtaact tcgactttcg ccagtttttc gtagtagcac gccagggcgc tatgatccgc
     2941 cgttcctaaa ccatctgcac gcagtgcctg catcatctcc ataaccgcag ctgtgagcag
     3001 cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc gccagatcct taatatgcag
     3061 atcaatgcgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag
     3121 cacggtactg cccgccagcc caccgcgaat tgcctgataa accaggtccg ggttaacgcc
     3181 cgctttggtt gccagcgtta acgcttctga catcgcagca atattcagcg ccacaatgac
     3241 ctgatttgcc agtttggtga cgttacctgc accgatttcc ccggtatgca ccacggaacc
     3301 cgccatcgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat
     3361 cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat
     3421 atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga
     3481 actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag
     3541 cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc
     3601 agcgatcgct ttagccgtag acgctgtttc tgcacctgca gcaatcacgt cagcaatagc
     3661 ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat
     3721 tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat caaccatctc
     3781 tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttctgagt ggcagagcgg
     3841 aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc
     3901 gcatcggctt cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct ggcacggtta
     3961 aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg cattgccgag atggcccaat
     4021 gccgcagcca gatcgctggg gccgacgaag ataccgtcta caccttcggt agcggcaata
     4081 gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt aatgttcttg
     4141 ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggtgcggtg agaaacggag
     4201 acgccgcgaa tgccttccgg cgggtaacga gttgatgcca ccgcctgctc tgcttcctct
     4261 tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata
     4321 attaccggct cgttggtcgg cactcgcact actggcgcgc tggcgctgcc tttcagggcc
     4381 attaactgcg gaataaacgt ggaaatatcg tttggcgcat gttcgccatc cagcaccagc
     4441 cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag
     4501 caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg gaaaacatcg
     4561 ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact
     4621 acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca
     4681 ttgaaggagt gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca
     4741 acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt cggcgcggtg
     4801 tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc cagcgccatc
     4861 agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt ggaaaccagc
     4921 aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc
     4981 agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgccggaatc
     5041 gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg
     5101 aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc
     5161 aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc
     5221 gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc
     5281 agctcttccg cagacatacg tgggtgatct gtcgggttat gaatcaactt gatccacagc
     5341 gccgtcagca caaaaccaat cccccccata acggtgaaga cgtgctccca gccccaagcg
     5401 aaagtcagcc agccaagcag cggcgaaaag agcgccagcg agaaatattg cgccgagtta
     5461 aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg
     5521 ttcgccggga atgatggcgc ttccgagaac cccagcataa agcgcataaa gaacatggag
     5581 atccctgccc aggccagcgg gaacatatca acaaagcctt gcaggaaggt gaacagcgac
     5641 cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag caaccagccg
     5701 ccggggattt gcatcagcaa gtaggcccag ccaaaagcag agaagatgta acccatcgaa
     5761 accgcactta actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga
     5821 tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata gctggtatgc
     5881 acgcctttct tttttacgtc aacggtgtcc agaatcattt tatttacctc gggtacttat
     5941 gctgattttt attattatgg ggaaggtgtt atttatgagt tttatttatg ccgttacgac
     6001 aatgaactcg ggaattagta taagcagcgg gagaataata atcattgtgc aaatgctaat
     6061 ttaatcagta ctatttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc
     6121 acagatttgc gctttacctt accgggcggc actgcaatcc ctgaaatgat tgacattgat
     6181 cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaagtc atttattttt
     6241 aaatgagacc agatcctcat tttaataacc cctggctgga gaatattgca caatggccaa
     6301 catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa
     6361 tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct
     6421 ggaattaatt gaacatactc cccaggggca taaagtcgca ttgctggaca ttccagctaa
     6481 tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa tcccacgcgg
     6541 aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc
     6601 actggcaacc aaagtcccgg cccccttacc gccgctggaa ggatacacct ttgagggcta
     6661 tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca
     6721 ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa
     6781 atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggctgtg gcgtggcgat
     6841 taacgcaccg gcggcagttg tgcctattcg taccatccac aatatttcgc tgaaccctaa
     6901 ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc ctgagcgcct
     6961 gctggtcggc acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt
     7021 gcaggatgaa aagcatgtcg gctttcagtc catggtcgag gatattttgc aggtcgccga
     7081 acgccatcta caaaaactga atcaacggca gcgagaaacc tgcccggctt cagaactggt
     7141 cgttggtgtg cagtgcggtg gcagcgatgc gttttctggt gtaacggcaa acccggcggt
     7201 tggctatgcg tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt cagaagtaac
     7261 ggaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag aggtcggcaa
     7321 acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg
     7381 cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtgg tggagaaggc
     7441 actcggctcc attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt cgcccggtca
     7501 acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg
     7561 cacgcaacag gtggcttcgg gtatcaccgt acaagtgttt acgaccggcc gtggtacgcc
     7621 gtacggcctg atggcggtac ccgtaattaa aatggcaacc cgcaccgagc tggcgaaccg
     7681 ctggtttgat ttaatggata ttaacgcggg cactatcgct accggcgaag agagcattga
     7741 agaggtgggc tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac
     7801 cttctcggat caatgggggc tgcataacca gctggcggtg tttaacccgg caccggtgac
     7861 ctgatttctt ttggaacgtc cctcgcaaaa catggcctta gtgccatgtt tttattgttt
     7921 aaagccccca cgtccattaa taatgcattt gcattacctt agttcaagct tataatttga
     7981 gcagaaaaca ggctgtaaaa ggacaatgaa tcatgcccgc taatgctcgc tctcacgctg
     8041 tactgaccac tgaatcaaag gtcacgatac gcggacaaac aactatcccc gcgccagtgc
     8101 gtgaggcctt aaaa
//
LOCUS       DAEEBB010000169         8104 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEEBB010000169 DAEEBB010000000
VERSION     DAEEBB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.169"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(101..796)
                     /locus_tag="KLF07_003672"
     CDS             complement(101..796)
                     /locus_tag="KLF07_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBD9833585.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="KLF07_003673"
     CDS             complement(789..2216)
                     /locus_tag="KLF07_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBD9833586.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="KLF07_003674"
     CDS             complement(2227..2946)
                     /locus_tag="KLF07_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBD9833587.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="KLF07_003675"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="KLF07_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBD9833588.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="KLF07_003676"
     CDS             4554..5879
                     /locus_tag="KLF07_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBD9833589.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="KLF07_003677"
     CDS             5988..6224
                     /locus_tag="KLF07_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD9833590.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="KLF07_003678"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="KLF07_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBD9833591.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="KLF07_003679"
     CDS             complement(6989..7858)
                     /locus_tag="KLF07_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBD9833592.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAEEBB010000170         8054 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEEBB010000170 DAEEBB010000000
VERSION     DAEEBB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.170"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            103..468
                     /locus_tag="KLF07_003680"
                     /pseudo
     CDS             103..468
                     /locus_tag="KLF07_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="KLF07_003681"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="KLF07_003681"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBD9833593.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="KLF07_003682"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="KLF07_003682"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBD9833594.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="KLF07_003683"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="KLF07_003683"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBD9833595.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="KLF07_003684"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="KLF07_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBD9833596.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /locus_tag="KLF07_003685"
     CDS             complement(5599..7035)
                     /locus_tag="KLF07_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBD9833597.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="KLF07_003686"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="KLF07_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBD9833598.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2113 c         1910 g         1958 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgcttcagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAEEBB010000171         7969 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEEBB010000171 DAEEBB010000000
VERSION     DAEEBB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.171"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..239
                     /locus_tag="KLF07_003687"
     CDS             <1..239
                     /locus_tag="KLF07_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBD9833599.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="KLF07_003688"
     CDS             279..1142
                     /locus_tag="KLF07_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBD9833600.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="KLF07_003689"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="KLF07_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBD9833601.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="KLF07_003690"
     CDS             2679..4037
                     /locus_tag="KLF07_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBD9833602.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="KLF07_003691"
     CDS             4180..5130
                     /locus_tag="KLF07_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBD9833603.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="KLF07_003692"
     CDS             5140..6522
                     /locus_tag="KLF07_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBD9833604.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="KLF07_003693"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="KLF07_003693"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBD9833605.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1723 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctgt
//
LOCUS       DAEEBB010000172         7944 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEEBB010000172 DAEEBB010000000
VERSION     DAEEBB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.172"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            111..443
                     /locus_tag="KLF07_003694"
     CDS             111..443
                     /locus_tag="KLF07_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBD9833606.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="KLF07_003695"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="KLF07_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBD9833607.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="KLF07_003696"
     CDS             complement(2281..2598)
                     /locus_tag="KLF07_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833608.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="KLF07_003697"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="KLF07_003697"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBD9833609.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="KLF07_003698"
     CDS             complement(3985..4758)
                     /locus_tag="KLF07_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD9833610.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="KLF07_003699"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="KLF07_003699"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD9833611.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="KLF07_003700"
     CDS             complement(5952..7307)
                     /locus_tag="KLF07_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBD9833612.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="KLF07_003701"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="KLF07_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBD9833613.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="KLF07_003702"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="KLF07_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBD9833614.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAEEBB010000173         7776 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEEBB010000173 DAEEBB010000000
VERSION     DAEEBB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.173"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1780)
                     /locus_tag="KLF07_003703"
     CDS             complement(<1..1780)
                     /locus_tag="KLF07_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBD9833615.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="KLF07_003704"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="KLF07_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD9833616.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="KLF07_003705"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="KLF07_003705"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBD9833617.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="KLF07_003706"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="KLF07_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBD9833618.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="KLF07_003707"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="KLF07_003707"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBD9833619.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="KLF07_003708"
     CDS             complement(5968..7005)
                     /locus_tag="KLF07_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD9833620.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="KLF07_003709"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="KLF07_003709"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD9833621.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAEEBB010000174         7741 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEEBB010000174 DAEEBB010000000
VERSION     DAEEBB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.174"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            20..517
                     /locus_tag="KLF07_003710"
     CDS             20..517
                     /locus_tag="KLF07_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD9833622.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            514..984
                     /locus_tag="KLF07_003711"
     CDS             514..984
                     /locus_tag="KLF07_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD9833623.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1073..1585)
                     /locus_tag="KLF07_003712"
     CDS             complement(1073..1585)
                     /locus_tag="KLF07_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBD9833624.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1780..2544
                     /locus_tag="KLF07_003713"
     CDS             1780..2544
                     /locus_tag="KLF07_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833625.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2495..3898
                     /locus_tag="KLF07_003714"
     CDS             2495..3898
                     /locus_tag="KLF07_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBD9833626.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3898..5364
                     /locus_tag="KLF07_003715"
                     /pseudo
     CDS             3898..5364
                     /locus_tag="KLF07_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5255..5998
                     /locus_tag="KLF07_003716"
     CDS             5255..5998
                     /locus_tag="KLF07_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD9833627.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            6002..7222
                     /locus_tag="KLF07_003717"
     CDS             6002..7222
                     /locus_tag="KLF07_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD9833628.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7226..7732
                     /locus_tag="KLF07_003718"
     CDS             7226..7732
                     /locus_tag="KLF07_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833629.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2060 a         1845 c         2110 g         1726 t
ORIGIN      
        1 aggctgcgag aggtgaataa tgtcgccatc attacgcaag gctgttgcag ttgctattgg
       61 tggcggggct gttgctatag catctgtgtt aatcactggc ccaggtggta acgatggtct
      121 ggaaggtgtc agctacatac tatacaaaga tatcattggt gtatggactg tatgtcacgg
      181 acacaccgga aaagacatca tgcctggtaa aacgtatacc gaagcagaat gcaaagctct
      241 cctgaataaa gaccttgcca ctgtcgccag acaaattaac ccgtacatca aagtcgatat
      301 accggaaaca acgcgcggcg ctctttactc gttcgtttac aacgtgggcg ctggcaattt
      361 cagaacatcg acgcttcttc gcaaaataaa ccagggctat atcaaaggcg catgtgacca
      421 gctatgtcgc tggacatatg ctggcggtaa gcaatggaaa gggctgatga cccgtcgtga
      481 gattgagcgt gaagtctgtt tgtgggggca gcaatgagca gagtcaccgc gattatctcc
      541 gctctggtta tctgcatcat cgtccgcctg tcatgggctg ttaatcatta ccgtgataac
      601 gccattacct acaaagagca gcgcgacaaa aacgcaagag aactgaagct ggcgaacgca
      661 accattactg acatgcagca gcgccagcgt gatgctgatg cactcgatgc taaatacacg
      721 aaggagttag ctgatgcgaa agctgaaaat gatgctcttc ggcgcaagct tgataatggt
      781 ggtcgggtgc tcgtcaaagg aaaatgccct gtgccatcct cagccgaaac ctccagcgcc
      841 tccggcatgg gcaatgatgc caccgtcgaa ctctctccag ttgctggacg aaacgttctc
      901 ggtatccggg acggaatcat cagcgaccag acagcattga gaatgcttca ggagtacatc
      961 aggatccaat gccttggggg gtagcggtaa ttttactcat tatccttcaa atcagattct
     1021 gttgtcagag gaatggggga agctggattc gcaagagctt tttatgggca gtttagctaa
     1081 aaaaaggcaa cacgaagcta aaactaccaa cataaagtgc tgaccagcta gcagctgaaa
     1141 tcagactcac tgtatacact ttcactggat gaagttgcaa cattcctgcc accaaaggag
     1201 caatataacg cagcacggcg ataaagcgcg aagtgaaaag tattgacaca gagttatttt
     1261 gcaactgaag gcgaacccgt tctattgtat tggaacggct tgatattata catgcaagtt
     1321 gagggatatg cccaattatt ataccaagat ggtaattaac gatagtaccg caccatgcgc
     1381 cactcatgac cgtgattcca gcttcccatg gtgaaagagt cgtcttgctg actgtaatta
     1441 cagtggccat cataattgat gctggtggca atatcgccga aatgagtaaa gtggattttg
     1501 tgaacgctat tataaaaaga agcccccata ggcgatatgg atgaagtaca aaatagttca
     1561 tcaaagcatt tatccactcc atcaatgtct ttccttctgt gaatttttct ttgctattta
     1621 accgtgatac gacttaattt aggcttaaac accttacatt tacaaggtac tcctgtgggg
     1681 ggccctgcca cggagcgtcg caggagtgag tgatgaggaa gaattaaagc aacctattgt
     1741 gacaggcagc gataacagta aatcacagga agcataatca tggcaaaacc ggactgggag
     1801 gccatcgaat cggcataccg ggccggagtc cttagcctcc gtgatatagg cgagaaatac
     1861 ggcgttacag aaggggctat caggaagagg gccaaaaagc ttggttgggc acgcagtggc
     1921 ggtacgcagg tttgcaaaaa tggtacgcaa aaaaggaaag tgcgtaccag cagaaagcct
     1981 gccattactg gccttacaca aaaaagtacg caactaaaaa cagaatctac accggatacg
     2041 aaaccgatac gcggaatgcg taccgatccc ccgactaacc cattccaacc cggtaaccag
     2101 caggcattaa aacacggtgg ttatgcccgt cgccttctgc tcaaagatga ggtgatagag
     2161 gacgctaaag cgttgacgct cgaggacgaa ttatttcgcc ttcgtgctaa caaccttgtc
     2221 gctgcagaga atattggtcg gtggttggtg tcgctggaag atgctaatgg ggaccaggaa
     2281 aggaagatgc tgattgaaaa tatcagcgcc gccgagaaag caatgatgcg caatacagtt
     2341 cgtattgagt ccatcgttgg cacgcttgcg acggtaggaa aaatatttgc tgatacagcc
     2401 taccgcaagg ccgccactga taaggtgtct ctggaggctg atcgtcttcg ccgtgatgca
     2461 ggtattgatg atggcaatgg agagcgtgac ctcaatgact tctactctga catccaaacc
     2521 gacgctgaat ccggctttac gtagtttctg gactatgcgg gcacgtaaca aagtgcttta
     2581 tggtggtcgg tcatcgtcaa aatcatggga tgccgctggc attgccatat ttctgtcgaa
     2641 taaatacacc ctgcgttttt gttgtgcccg tcagatccag aataaaatcg aagagtcggt
     2701 gtataccctg ctcaaaattc agatagacag gtttggtctg cggcaccgtt tccgtattct
     2761 gaacaacaaa atcattaacc gggttactgg ctcggaattt gttttttatg gattatggcg
     2821 caacatcgaa gaaattaagt cactggaggg gatcgatgtg ttgtggctgg aagaagccca
     2881 cgcactgacg gaataccagt ggaaaattct ggagccaacg atccgtaaag agggttcgga
     2941 atgctggttc atattcaacc ccggacttgt tactgatttc gtctggcgca acttcgttgt
     3001 tgatccgccc gaaggcactc tcatccgcaa aattaactat gacgaaaatc cgtttctgtc
     3061 tgacaccatg cttaaggtta tcgacgcggc gcgacgccgt gatccggatg gttttaaaca
     3121 tgtgtatgag ggcgttccgg agtctgatga tgatgcggca atcatcaaac tgtcttggat
     3181 agaagccgca gtggatgcgc acaaaacgtt aaatttcgaa cccagtggaa gaaagcgtat
     3241 tggctttgac gtggctgaca gtggtacaga taagtgcgct aacgtttacc gtcacggatc
     3301 cgttgttttc tgggccgacg aatggaaggc caaagaagat gaattactga agagctgcca
     3361 gcgtacttat caggcggcgc tggagcgtga agcagatatt gtttacgact ctatcggtgt
     3421 tggtgcgtct gccggtgcta aattctctga aattaacgct gaccggaaga gcgagaacgc
     3481 atacgcgcga cgtgtgaatt accagaggtt taacgccggt gctggtgtgc atgagccaga
     3541 tgacgagtac aacggcatcc ccaacaaaga ctttttcgca aatcttaagg ctcaggcatg
     3601 gtggctggtg gctgaccgtt tcagaaatac gtttaacgcc attaacaacg gagaacagta
     3661 tcctgtggat gagctgatca gcatagattc tcgttgtccg ttgcttgaaa agctgaaact
     3721 ggaactgaca acacctcatc gtgatttcga ccgtaacgga cgtgtgatgg tcgaaagtaa
     3781 aaaagacctc gcaaaacgcg agataccgtc accaaacgtt gctgatgcat tcattatggc
     3841 cttcgcgcca atcgatacat cgctggatat ctgggaacag ctggggagac aggcctgatg
     3901 gcacgaaaca aacaagccct gcggcgaact gtgcaggcca cagctgatgg ttatgagaat
     3961 tttattgccc gcgtagggat gcagacacct aaccagcact cagcatccac ctaccgggct
     4021 aatttcacca gtcgtaaccg catgctggtg gaatggtcct atcgttcatc ctagatcatc
     4081 ggcgaagcag tcgatgctat cccggatgat atgacccgca aaggcattcg catcacttcg
     4141 gaaattgatg caaaagatcg tggcattctc gaatcacaac tggatgagtt gcaaatctgg
     4201 gatgcgctga atgacgtgct gaaatggtcg cgcctctacg gcggcgcggt gggtttcatc
     4261 atgattgagg ggtaggcacc aatgaccccg ctgcgacccg aaaccatcgg taagggcaag
     4321 tttaagggga ttctcccgct cgaccgctgg atggtcgacc cggcactgac ccgccgcatt
     4381 aaagatatgg ggccggacct gggtaaacct gagttttacg atgtggtgac cacagcaacg
     4441 ggaattcctg cctggcgcat tcatcacagt cgcctgattc gctttgatgg cgtcacgttg
     4501 ccatttcagc agaagatgac cgagaacgaa tggggaatgt cggttgtaga gcgtatctgg
     4561 gatcgtctta ccgcgttcga cagcgctact gtcggcgcgg cgcagctggt ctacaaggcg
     4621 catttgcgta cctacagcgt ggagaagcta cgcgagctta tcgcacttgg tggtcctgcg
     4681 tatgaagcgt tgctgaagaa tatcgacctg attcgacagt tccagagtaa tgaaggcatg
     4741 acgctcatgg actcgcggga taagtttgaa acccatcagt acagcttcag tggtctggat
     4801 gacatccttt cgcagtttgt agaacagatt agtggcgctg ttggtatccc actggtgcgg
     4861 ttgttcggac agtccccgaa aggattttct accggtgatg cagaccttgc caactattac
     4921 gatcgcatca gttcgttgca ggagaggcgt ttacgtcttc cggtgcggcg gatactggac
     4981 atcatgcatc gttcggaact tggcaagccg ctgccggacg atttcacgtt tgagtttaac
     5041 ccgctctggc aaatgtctga tgtcgatcgc tcaacggtgg cgttaaacac caccaacgca
     5101 atcagtacgg cgctgggtga tggtctgatg acactgaaag ccgctatgac tgatttgcga
     5161 gaaaattctg acgtaaccgg catcggggca tccattaccg acgaggacat cgagaatgcc
     5221 gaagatgaag cgccgcccgg catcggcgaa cctgatgacg aaccgcagga gccgtcaggc
     5281 ggaaatccgg tatcgaacca gcctacgcag gatagcgagg gcggtcggag acatcgtaaa
     5341 tggtcgctac gatggttcaa atgacagtat cacggaaatt attgcggcgc tggagcgtta
     5401 cagtgaaatc atcaccccct gggcgacaaa ggtcgcggaa aactttactg cggacctaac
     5461 ccggcagaac gagaaagttt ggcggcaaca cagcaagaac atcagtcgcg agctccgcaa
     5521 tcttgtggaa agcgctcctg tgggccaggt gatgcaatcc atcatcgccg aacaggtcaa
     5581 gtacatcaaa tctctgcctc tcgaggccgc agacagggtg tacgacatcc agaacaaagc
     5641 gatagaggct gttgtcaccg gtggtcgggc ggagcagttt gctaaagaga ttgcatctac
     5701 cggcgatgtt gctaagtcta gggccgatct gattgcccga acggaactgg gaagagcaac
     5761 gggcgcgctg gatatggccc gagcgatagc tattggctcg gatggttata tttggcgtac
     5821 agccgatgat ggcgatgtcc gagattccca cgatcacatg aaaggtaaat tcgtccgctg
     5881 ggattcacct ccaactttgg acggcatgac cggccacgcg ggcgagctgc caaactgccg
     5941 ctgctataaa gagatcgtgt ttgttcgcgt tccattcgca atgaaaaggg cagcataacc
     6001 catgaaatac ttttttgaga ccaggctcgg ggaaacccga taccgcctgg ctgacggctc
     6061 gttgctgtgc aaagacgtgc cgataggacg aacaggtaag cagctctatg gtgctgatga
     6121 cctgccaaaa ctgaaacccg ataagttcgg tgaaatagtc gtcacgcgtt ctcctgagca
     6181 ggtattccat ccggccacgc ttgcctcatt cgaagggatg agcatcacga tcctgcatcc
     6241 tgaagatgaa aacgggaatg tgcggctggt aaatcccgag aactggaaag agcttgctgt
     6301 cgggcacctc cagaatgtcc ggcgcgggac gggtgagcag tctgatttga tgctggctga
     6361 ccttatcgtc aaagacgaaa gcgccattca gcttatcgaa gatggcctgc gcgaagtgtc
     6421 gtgcggctat gacgcggagt acgagcagac cgagccaggt aaagccgagc aggtcgatat
     6481 taccggaaac catgtggctc ttgtccctaa aggcagagcc ggaaatcgtt gtgcaattgg
     6541 agacagagac acaatggcaa atcaaaagaa aaactggtgg aaccgcatgc gtgcggccat
     6601 caagacagga gatgccgaca ccatgaacga actggtggag tcggctcccg catcggttac
     6661 aggagatgag ggggatttgc cgcagggcgt taatctcaac atcaacctgt ccccgcagca
     6721 accactaccg gacaaagcac cagagatggg cggagatcca accggcgaca gtgatgatga
     6781 cctcaaaaca ttactgaaag ccctgctggc taagctggaa agaaatgcca cgggcgataa
     6841 cgataataag cctgacgata atccgaccgg tgacggcgag gacgatgaag aggaaaccac
     6901 gattactggt gactcagcct ggcgtgccga agttatcgtt ccgggtatcg atctgagccg
     6961 taagatgaaa ccgaccgcgt tcaaacgcga ggttctggct tctgctgaca aaacgctggt
     7021 tcgccagata gtcggtgatg cggatatccg caaattaccg aaacaatcgg tcgacatggc
     7081 gtttaatgcc gtgtctgagg ttgccaaagg gcgaaacacc cgcgccacca ccggcgatgc
     7141 acagcgccta aacatgggca tgaccagtat cgcttccctg aacaaacaaa acgctgaatt
     7201 ctgggcaaac cgtaaagggt aaaaaatgaa taatgtattt ctgtaccgga tgcctgttgg
     7261 cattgccggg gctgtctctc gcccgcagga cttaaccgtc gaaccggtgg tccttaaatc
     7321 cgataacgcc ttcgctgcct atggcctggc tggtaaatac gatgatgacg gttttttcgt
     7381 gccgctggca gatggtgata ccgcagacaa ggtgaagggg atctacgtgc gcccttatcc
     7441 gaccacgtcg cagccggaca tggttcgcca ggtgggaaca ggcaagaact tcccgggcga
     7501 cgccatgaag cgtggctacg tgaccgttaa tctcggttct gattttgatg ccagcactat
     7561 caaaaaaggc gatccggtat acgttgtcgt ctccactgat gaatccatca aagtgccgct
     7621 gggtggattc atgtccacgt cagtcagtgg caaaaacgtg gtgctgacca acgctgaatt
     7681 cacaggtgcc ggtgatgctg acggcaatgc agaaatttcc tggaagattt aaggaacaga
     7741 c
//
LOCUS       DAEEBB010000175         7715 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEEBB010000175 DAEEBB010000000
VERSION     DAEEBB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.175"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..643
                     /locus_tag="KLF07_003719"
     CDS             <1..643
                     /locus_tag="KLF07_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD9833630.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="KLF07_003720"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="KLF07_003720"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBD9833631.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="KLF07_003721"
     CDS             2474..2986
                     /locus_tag="KLF07_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBD9833632.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="KLF07_003722"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="KLF07_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBD9833633.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="KLF07_003723"
     CDS             5579..5782
                     /locus_tag="KLF07_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD9833634.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="KLF07_003724"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="KLF07_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBD9833635.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="KLF07_003725"
     CDS             complement(6827..>7715)
                     /locus_tag="KLF07_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HBD9833636.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAEEBB010000176         7623 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEEBB010000176 DAEEBB010000000
VERSION     DAEEBB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.176"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..96
                     /locus_tag="KLF07_003726"
     CDS             <1..96
                     /locus_tag="KLF07_003726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD9833637.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="KLF07_003727"
     CDS             complement(152..487)
                     /locus_tag="KLF07_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBD9833638.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="KLF07_003728"
     CDS             complement(491..835)
                     /locus_tag="KLF07_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBD9833639.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="KLF07_003729"
     CDS             complement(837..1010)
                     /locus_tag="KLF07_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833640.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="KLF07_003730"
     CDS             1111..1296
                     /locus_tag="KLF07_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833641.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="KLF07_003731"
     CDS             1257..1376
                     /locus_tag="KLF07_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBD9833642.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="KLF07_003732"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="KLF07_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBD9833643.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="KLF07_003733"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="KLF07_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBD9833644.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="KLF07_003734"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="KLF07_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBD9833645.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="KLF07_003735"
     CDS             4176..4544
                     /locus_tag="KLF07_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBD9833646.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="KLF07_003736"
     CDS             complement(4652..5053)
                     /locus_tag="KLF07_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBD9833647.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="KLF07_003737"
     CDS             5295..5588
                     /locus_tag="KLF07_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBD9833648.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="KLF07_003738"
     CDS             5660..6319
                     /locus_tag="KLF07_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBD9833649.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="KLF07_003739"
     CDS             6411..6857
                     /locus_tag="KLF07_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBD9833650.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="KLF07_003740"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="KLF07_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HBD9833651.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAEEBB010000177         7491 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEEBB010000177 DAEEBB010000000
VERSION     DAEEBB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.177"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="KLF07_003741"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="KLF07_003741"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBD9833652.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="KLF07_003742"
     tRNA            647..722
                     /locus_tag="KLF07_003742"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="KLF07_003743"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="KLF07_003743"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBD9833653.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="KLF07_003744"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="KLF07_003744"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBD9833654.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="KLF07_003745"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="KLF07_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBD9833655.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="KLF07_003746"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="KLF07_003746"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBD9833656.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="KLF07_003747"
     CDS             complement(5917..6249)
                     /locus_tag="KLF07_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBD9833657.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="KLF07_003748"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="KLF07_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBD9833658.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1869 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAEEBB010000178         7428 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEEBB010000178 DAEEBB010000000
VERSION     DAEEBB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.178"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..98)
                     /locus_tag="KLF07_003749"
     CDS             complement(<1..98)
                     /locus_tag="KLF07_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HBD9833659.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
     gene            complement(123..1223)
                     /locus_tag="KLF07_003750"
     CDS             complement(123..1223)
                     /locus_tag="KLF07_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBD9833660.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="KLF07_003751"
     CDS             complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="KLF07_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBD9833661.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2371..2937)
                     /locus_tag="KLF07_003752"
     CDS             complement(2371..2937)
                     /locus_tag="KLF07_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD9833662.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3153..4889
                     /gene="argS"
                     /locus_tag="KLF07_003753"
     CDS             3153..4889
                     /gene="argS"
                     /locus_tag="KLF07_003753"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBD9833663.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5066..5428
                     /locus_tag="KLF07_003754"
                     /pseudo
     CDS             5066..5428
                     /locus_tag="KLF07_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5432..6502)
                     /locus_tag="KLF07_003755"
                     /pseudo
     CDS             complement(5432..6502)
                     /locus_tag="KLF07_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="KLF07_003756"
     CDS             complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="KLF07_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBD9833664.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1946 c         1776 g         1786 t
ORIGIN      
        1 tcttctgccc atgcggggag cggtgctgcc gatgtcacca cctgcgctcc agcggcaata
       61 ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag
      121 gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc
      181 agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca
      241 ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca
      301 agtttttcag cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca
      361 ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt
      421 accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc
      481 gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc
      541 tgtttgccac tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg
      601 cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc
      661 tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca
      721 atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc
      781 tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac
      841 gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga
      901 gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta
      961 gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca
     1021 cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa
     1081 gatttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct
     1141 ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac
     1201 ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca ccggaatcat
     1261 tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa
     1321 ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca
     1381 gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga
     1441 atataaaaaa tattattagc acaccgctat gttcactgtc acccgctcaa cagatcacgg
     1501 ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg
     1561 gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc
     1621 tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc
     1681 gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc
     1741 ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag
     1801 tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg
     1861 gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc
     1921 agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac
     1981 atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc
     2041 attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa
     2101 cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag
     2161 caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc
     2221 acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat
     2281 tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct gtaacagcaa
     2341 atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg caacgatctc
     2401 ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg
     2461 attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga tccccggtag
     2521 gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc
     2581 cagcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca
     2641 cggtagctcc acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa
     2701 cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa
     2761 cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc
     2821 agtcagcgga gtgatattca accccagacg gagggaaagt tcatctaatg cgtgggtaaa
     2881 tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat
     2941 cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc aaccaaccgc
     3001 tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct
     3061 gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat
     3121 ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa
     3181 aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc
     3241 attcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa
     3301 aactgggtat gacaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg
     3361 gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg
     3421 cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gtttctacgc
     3481 cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcatg
     3541 tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg
     3601 gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga
     3661 ttgcatggct ggaaaagcag cagcaggaaa acgccggtga tgaaatggag ctggctgacc
     3721 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     3781 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     3841 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
     3901 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     3961 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     4021 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     4081 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     4141 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     4201 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     4261 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     4321 aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg gcagaaaaga
     4381 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     4441 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     4501 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     4561 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     4621 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     4681 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     4741 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     4801 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
     4861 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
     4921 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
     4981 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
     5041 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
     5101 ctcttgccag gcttacgcag aagatacggt tatagaaaac gaccatgaca tttcagccat
     5161 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     5221 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
     5281 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg aacaacttaa
     5341 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
     5401 cctttcaaat gaggacgttc aggatttaat gtacatcgtt ccacgtcgct tcgacaacgg
     5461 tattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc
     5521 gtatccacca ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca
     5581 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     5641 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     5701 cgatgacccc cgcggcggta aaaatcacca gcgccggaat ttgtgccacc agaccatcgc
     5761 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     5821 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     5881 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca
     5941 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt
     6001 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     6061 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca
     6121 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc
     6181 ctgccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa
     6241 gtgccagacg caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg
     6301 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     6361 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     6421 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca
     6481 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     6541 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     6601 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     6661 ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     6721 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     6781 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     6841 ttattgacaa tgacatcagc tttcggcaca tcggccatca tccgacgccg tgcagcagct
     6901 cgctgcatct gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca
     6961 tcacgaatat cctgccgcga catacgcagc tttttcaggt ggctgaagat ttggaaaaag
     7021 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagcccaacc
     7081 agatccatcg cattacccat ggcggtagtc ggagactcgg ccattaagcg catcatctgc
     7141 ggccagtgat gccagagata aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     7201 gccttaagca actccgcgcc agtctgtgcc gagaacatcc gtttaatgcc cggtagcggg
     7261 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc ccccagcatg
     7321 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg
     7381 gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtca
//
LOCUS       DAEEBB010000179         7371 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEEBB010000179 DAEEBB010000000
VERSION     DAEEBB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.179"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            33..719
                     /locus_tag="KLF07_003757"
                     /pseudo
     CDS             33..719
                     /locus_tag="KLF07_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1052..1927
                     /locus_tag="KLF07_003758"
     CDS             1052..1927
                     /locus_tag="KLF07_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HBD9833665.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1935..2711
                     /locus_tag="KLF07_003759"
     CDS             1935..2711
                     /locus_tag="KLF07_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HBD9833666.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2880..5141
                     /locus_tag="KLF07_003760"
     CDS             2880..5141
                     /locus_tag="KLF07_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBD9833667.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5210..6385
                     /gene="senB"
                     /locus_tag="KLF07_003761"
     CDS             5210..6385
                     /gene="senB"
                     /locus_tag="KLF07_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HBD9833668.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKK
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6911..>7371)
                     /locus_tag="KLF07_003762"
     CDS             complement(6911..>7371)
                     /locus_tag="KLF07_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBD9833669.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         2060 a         1728 c         1722 g         1861 t
ORIGIN      
        1 tgaaaaacaa acgcccctga aatcatgtcc agatccggca gaaccagcct catgcgcagt
       61 tctggctggt ttttgacgtt gatcgcgagg gggcagcgat tgactggagc gaccggaacg
      121 ctcccgcgcc gaacatcacc gtaaaaaatc ctgtgaacgg tcatgctcac ctgctctacg
      181 cgctcaatat cgccgtgaga accgcgccgg acagctcggt taaggcgctg aaatacgccg
      241 ctgcgattga acgttcgctg tgtgagaaac tttgtgctga tgtgaactac agcggactga
      301 tttgtaaaaa tccgttccac ctggaatggc aggtgatgga gtggcgagag gaagcctata
      361 ccctcgatga actggctgat tatctcgatt tgagcgcctc agcgcgccgt agcatcgata
      421 aacattacgg gatggggcga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca
      481 gggcgattcg tcagggctgg cccgaattct cacagtggct tgatgccgtg attcagcgtg
      541 tcgaaatgta caacgcatcg cttcccgttc cgctttcacc gcctgaatgt cgggctattg
      601 gcaagagcat tgcgaaatac acgcacagga acttcacgcc ggaaactttc gcacagtatg
      661 tggctgatac gcacacatta acgtacgttt tcgttccatt ggccctcacc ccccgataac
      721 gacccaggta gcgcgccgta ttccccccgc cggccgtttt cttcgacatg tacatcaggc
      781 gcggaactca tcggggcgcg ggaatgaaat cccattcaga ctccggatag attcaagcaa
      841 gcccacttat cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat ccgttttcca
      901 agcggacccc ataatatcgg ctggtaaaca tcactcgcta gtgcattctt tttgttggta
      961 atgataatca tttacattca ttctttgatg ttttatcata attaatgttt tgtcaataaa
     1021 tatatggaaa catattgttt cagatgggaa tatgagaaag ttcatcctta tttctatgat
     1081 aacgttagct agtgttagtc tgggagcctg cagacagaat gtcaccataa agcaggatgc
     1141 gccagggcag aaagctttcc tgactgacgg gcagatttac gatttgcaca gtggcaaaat
     1201 tatttctagc agcgagcttt tggcggatct tgctaccgct cgacatctta ttattggtga
     1261 aaaacacgat aatgccgagc atcatcagat tgagctttgg cttattcaaa atttgctcat
     1321 ccagcgacca caggggagtg tgttgcttga gatgttgacg tcagagcaac agccccgagt
     1381 taaccaagtt aaatgttggc tgaaagataa tcctgtggtt cgagatagcc gtgttcagga
     1441 actacttaac tggcaaaaag ggtggtcctg ggagatgtat ggcgacatcg tcatgcagct
     1501 attacgtggg ccttacccgc tgctcaatgc caacattggc cgggagcaga tgttggcact
     1561 gtacaaaaaa aacgaatttc ctaaaggaaa aaaatccact gcacctgtgg tacaagaagc
     1621 tctgcgtgaa accatcatat ctatgcatga aggcaacctt gaaagccagc aactgacgtc
     1681 aatgctctct attcagcagc agcgggatcg ctacatggcc cgccagcttc tgagtgcccc
     1741 ggtaccgtcg ttgttgattg caggtggtta tcacgcgtca aaaagcatgg gcgtaccgtt
     1801 acacatggaa gatcttgcca ccggcaccca tccagtggtg ttgatgcttg cagaaaaagg
     1861 aatgaacatc acagtagatc atgctgacta cgtgtggttt gtagcgccag atacaacaaa
     1921 aaggtaaacc atggatgatg aatattctcc acttctcaca gtcggtaaaa tggagcagct
     1981 ggtttatctg ttctctactg cttcatggac tgattttcct tgcgtttatc tggcgttttt
     2041 ccgaagttca gcccgctatg tctcctgcac cggcgatcat gctgcaatgg gcagaaatta
     2101 tagaggctcc ttcttctcca ctttcgcttc cggtgggaat cgctcaacag gagtccgcag
     2161 tgacagaaga aaagcaacaa actgaggaca gacaacaacg tcctgtcact gaagacagtg
     2221 atgcaaccat tgaaataact aggaagaaaa agtcatccga tggagaaaag aaaaagacac
     2281 gtccacctcg taagataaag gctcaaacca gcgacagcaa ccccacagcc gtttccagta
     2341 atgctgcacc tcaggctctg gtcgaatcgt cccgtattgc tgcgcctttt aacagtgact
     2401 caacaaagcg agataattct gaggcatctt gggaaagcag agtaaaaggc cacctaaacc
     2461 gttataaacg ttaccctggc gatgccagaa aacgtgcacg aaccggcaca gccgtcgtga
     2521 cttttaccgt aaacacggaa ggcactattg tcagtagttt cttggaaatc tcttcaggaa
     2581 ccctttctct tgaccgcgaa gctattgctg ttttagaacg cgctcaacca cttcccaaac
     2641 caccgcctga aatattggag ggaggattat ttaaagtaaa aatgccgatt acctttaagt
     2701 tgaaggagta aagggataat tggccgttca agttcattta actgaaattt aattttcatt
     2761 tttatgctga acattttatc aaacttttcc actgtcgaaa aaatcaaaaa gacagtagaa
     2821 cataattaat aaacaaatag ttatacttat ctttattaat attataccca aggaaattta
     2881 tgaacgttat aaaactggct atcggctcag gcatattatt gctcagctgc ggtgcttact
     2941 cacaatccat cagtgaaaaa actaattccg acaaaaaagg agcggcagaa ttcagtccgc
     3001 tcagcgtttc tgtcgggaag acgaccagtg agcaggaagc tctcgagaaa acaggcgcga
     3061 ccagttcccg gacaacggac aaaaacctgc aatcacttga cgcaacagtg cgtagtatgc
     3121 ctggtactta tactcaaata gatcctggtc agggagcaat cagtgtgaat attcgaggca
     3181 tgagcggatt tggtcgtgta aacactatgg tcgatggtat tacccagagt ttttacggaa
     3241 cctctacctc cggaacaacg acgcatggtt caactaacaa tatggctggc gtacttatag
     3301 atcctaactt actggtagca gttgatgtta cacgcggtga cagcagtggc tctgaaggga
     3361 tcaacgccct tgccggtagt gcaaatatgc gtactattgg cgttgacgat gtaatattta
     3421 acggtaatac atatggcctt cgttcacgtt tctctgtcgg tagtaatggg ctgggacgca
     3481 gcggaatgat cgcccttggt ggaaaaagcg acgcttttac ggatacggga agcattggcg
     3541 ttatggctgc tgtgagcggc agttctgtgt actctaattt ctcaaatggt tctggaatta
     3601 acagcaaaga gtttggttat gataaatata tgaagcagaa ccccaaatcc caactgtata
     3661 aaatggatat cagaccagac gaatttaaca gcttcgaact ttccgctcga acctatgaaa
     3721 ataaatttac acgtcgtgat ataaccagtg acgactatta cattaaatat cattacaccc
     3781 ctttttctga attaattgac tttaacgtaa cggccagtac cagtcgcggt aatcaaaagt
     3841 atcgtgatgg ctcgctgtat actttctaca aaacctcagc gcaaaatcgt tctgacgcgc
     3901 tggatatcaa caataccagc cggttcactg tcgcggacaa tgacctggag tttatgctgg
     3961 gcagcaaact gatgcgtacc cgctatgacc ggaccattca ctcagcggcg ggcgacccga
     4021 aagcgaatca ggaatcgatc gagaacaatc cgttcgcacc ctccggccag caggatattt
     4081 cagcgctgta taccgggctg aaggttacgc gcggcatctg ggaggcagat ttcaatctca
     4141 actacacacg taacaggatc acagggtaca agcccgcctg cgattcacgc gttatctgcg
     4201 tgccacaggg tagctacgat attgacgata aagagggtgg cttcaaccct tcagttcagc
     4261 tttctgctca ggtaacacca tggcttcagc cgttcattgg ctacagcaaa tccatgcgcg
     4321 ccccgaacat ccaggagatg ttcttctcta attcaggagg cgcatccatg aacccattcc
     4381 tgaagcctga acgtgcagaa acctggcagg cgggttttaa cattgatacc agagatttac
     4441 tggtcgaaca ggatgccctg cgctttaagg ctctggcgta ccgcagcagg atccagaact
     4501 acatctacag cgagtcttat ctggtttgtt ctggaggtcg taaatgcagt atgcctgagg
     4561 tgattggcaa tgactgggag ggcattagcg atgaatacag cgacaatatg tacatctacg
     4621 ttaactcggc aagcgacgtt atcgcaaagg gcttcgaact ggagatggat tatgatgcag
     4681 gttttgcttt tggccgactc tctttcagcc agcagcaaac agaccagcca acctccatcg
     4741 ccagcaccta ctttggcgca ggggatatga ccgaactgcc cagaaaatac atgacgctgg
     4801 atactggtgt tcgcttcttc gataacgcgt tgaccctggg cactatcata aaatacacag
     4861 gcaaggctcg tcgcctgtcg cctgattttg agcaggacga acataccggc gcaataatca
     4921 aacaggattt gccgcagatc ccaacgatta tcgatctcta tggtacttac gagtacaacc
     4981 gcaacctaac actgaaactt tcggtacaaa acctgatgaa cagagattat tcggaggcgc
     5041 tgaataagct caacatgatg ccaggtcttg gtgacgagac ccacccagcc aattccgcgc
     5101 gtggcagaac atggatattt ggcggggaca ttcgtttctg atgacaattt ccgctaggtt
     5161 cagacaatac gttttctctc ttatgtcaat tttattgcag gaacgaaaaa tgaatatttt
     5221 cactttatcc aaagcaccgc tatacctgtt aatttcacta tttttaccca cgatggccat
     5281 ggctatcgat ccacctgaac gcgaactttc gcgatttgcc ctgaaaacga attaccttca
     5341 gtcccctgat gaaggcgtct atgaactggc gtttgataat gccagtaaaa aggtgtttgc
     5401 agcagtcacc gatcgtgtaa atcgtgaagc caataaaggc tatctgtatt cgtttaattc
     5461 agattcgctg aaagtcgaaa ataaatacac gatgccatac cgggcatttt cgctggcgat
     5521 aaatcaggat aaacatcagc tctatatcgg acacacccag tcagcgtccc tgcgtatcag
     5581 tatgtttgac accccaaccg gcaaactggt aagaaccagc gacaggttaa gttttaaagc
     5641 ggcaaacgct gcagattcgc gttttgagca ttttcgccat atggtttaca gccaggattc
     5701 cgataccctg tttgtgagtt atagcaatat gctgaaaacg gccgagggca tgaagcctct
     5761 gcataagctg ttaatgctcg acgggacgac gcttgcctta aaaggcgagg ttaaggatgc
     5821 ttacaaaggt acagcgtatg gtctgacgat ggatgaaaaa aaacagaaaa tctacgttgg
     5881 cggaagagat tacatcaacg aaattgatgc gaaaaatcag acgctgctgc gtaccatccc
     5941 gttgaaagat ccgagaccac aaatcacaag tgtgcagaat ctggcggtgg actccgcttc
     6001 tgaccgtgcc tttgtggtgg tattcgacca tgacgatcgt tccggtacaa aagatggact
     6061 ctatattttt gacttacgcg acggtaaaca gcttggctat gtgcacacag gagccggagc
     6121 taacgcggtg aaatacaatc cgaaatataa cgaactgtat gtcaccaact tcactagcgg
     6181 caccatcagc gtagtggatg ccaccaaata cagcatcacc cgtgaattta acatgccggt
     6241 ctacccaaac cagatggtgt tgtcggacga tatggatacc ctttacattg gcatcaaaga
     6301 aggctttaac cgcgattggg atcctgatgt gtttgtggaa ggagctaaag aacgtattct
     6361 gagcattgat ttgaaaaagt cgtgatcggg gggaaaaaac atgggataaa aactccgtta
     6421 ttccactaaa ctggccggac taggaaaagc aaacatctct gtcctgccgg tacgcctgtt
     6481 acgccgctcc atgaggctga ggaatctcca gaaaacgaga caggcacagc attgtacgat
     6541 caactgattt gtgccaataa ttaaatgaaa tcacagctat gcctgttcat ggggttctag
     6601 gccggaaccc cagatttttc cgattgtttg ttttctaact gattattcag tagttttaga
     6661 gggctgacgc tcagtggaac gaaaactcac gttaagaggt tttgactatg ataaaagtca
     6721 aagtaggggt gagtttggat atggaaatta ttgtactata aggagagcat gaaaacccct
     6781 ggggcagtca ctgaatgagg gaccagcagc ggtaaaataa ccagcataat ttctgcatgg
     6841 tcgattttta taccgtgaca ggctgttttt tatacttggg gtcagtttgg atatcgaaaa
     6901 ttattgtacg ttaaggttgt tttttgacct tgtcgatctt aactgatctc aaacgacgaa
     6961 acttacctga agtcggtatc cggctgtttt tccagatata atcgcctgtc agattgatat
     7021 gttcccaacc taatggtgat aaatgcgata atagttgagc atttattttc atgcccttac
     7081 gcttcagagc atcaaccgct ctttccatgt aaactgtgtt ccataaggaa atagccgccg
     7141 tcaataaagt caatccactg gcgcgataac tttgattttc cggtttccga tctctaatct
     7201 cccccaatcg atgcatgaat acagcacggg ccagagcatt acgggcttct cctttgttca
     7261 gcccggcctg gacacgtcgc cgcaatgccg gatcccgaaa ccagtccagc ataaacagtg
     7321 ttcgttctat acggccaatc tctctcagag ctttagccaa cccattttgt t
//
LOCUS       DAEEBB010000180         7239 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEEBB010000180 DAEEBB010000000
VERSION     DAEEBB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.180"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..337
                     /locus_tag="KLF07_003763"
     CDS             <1..337
                     /locus_tag="KLF07_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD9833670.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="KLF07_003764"
     CDS             337..666
                     /locus_tag="KLF07_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9833671.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="KLF07_003765"
     CDS             676..1374
                     /locus_tag="KLF07_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBD9833672.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="KLF07_003766"
     CDS             1379..2122
                     /locus_tag="KLF07_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9833673.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="KLF07_003767"
     CDS             2059..2661
                     /locus_tag="KLF07_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBD9833674.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGGGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="KLF07_003768"
     CDS             2722..6201
                     /locus_tag="KLF07_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBD9833675.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="KLF07_003769"
     CDS             6269..6868
                     /locus_tag="KLF07_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBD9833676.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="KLF07_003770"
     CDS             6932..>7239
                     /locus_tag="KLF07_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD9833677.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1726 c         2316 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg gaggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgt
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAEEBB010000181         7146 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEEBB010000181 DAEEBB010000000
VERSION     DAEEBB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.181"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            207..1073
                     /gene="folD"
                     /locus_tag="KLF07_003771"
     CDS             207..1073
                     /gene="folD"
                     /locus_tag="KLF07_003771"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBD9833678.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1075..1287
                     /gene="ybcJ"
                     /locus_tag="KLF07_003772"
     CDS             1075..1287
                     /gene="ybcJ"
                     /locus_tag="KLF07_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBD9833679.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1395..1916
                     /locus_tag="KLF07_003773"
                     /pseudo
     CDS             1395..1916
                     /locus_tag="KLF07_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1952..3337)
                     /gene="cysS"
                     /locus_tag="KLF07_003774"
     CDS             complement(1952..3337)
                     /gene="cysS"
                     /locus_tag="KLF07_003774"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBD9833680.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3511..4005
                     /gene="ppiB"
                     /locus_tag="KLF07_003775"
     CDS             3511..4005
                     /gene="ppiB"
                     /locus_tag="KLF07_003775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBD9833681.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4008..4730
                     /gene="lpxH"
                     /locus_tag="KLF07_003776"
     CDS             4008..4730
                     /gene="lpxH"
                     /locus_tag="KLF07_003776"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBD9833682.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4848..5357
                     /gene="purE"
                     /locus_tag="KLF07_003777"
     CDS             4848..5357
                     /gene="purE"
                     /locus_tag="KLF07_003777"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBD9833683.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5354..6421
                     /gene="purK"
                     /locus_tag="KLF07_003778"
     CDS             5354..6421
                     /gene="purK"
                     /locus_tag="KLF07_003778"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBD9833684.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6558..>7146)
                     /locus_tag="KLF07_003779"
     CDS             complement(6558..>7146)
                     /locus_tag="KLF07_003779"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD9833685.1"
                     /translation="TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKR
                     DGKYLRRVVASPQPRKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDL
                     AAALLAEQINADGLVILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVT
                     AVSGYVRSRGKPAWIGALSRIEETLAGEAGTCISL"
BASE COUNT         1594 a         1957 c         1909 g         1686 t
ORIGIN      
        1 cttagacaag gaaataagtc agttccagat ttacaacgcc atcatggacg aaaaatgaag
       61 ctttcaatct cagcgacggt gcgccccacc ttcgcaagag gtcgcttcac gcgataaatc
      121 tgaaacgaaa cctgacagcg cgccccgctt ctgacaaaat aggcgcatcc ccttcgacct
      181 acgtaacaga tggaatcctc tctctgatgg cagcaaagat tattgacggt aaaacgattg
      241 cgcagcaggt gcgctctgaa gttgctcaaa aagttcaggc gcgtattgca gccggactgc
      301 gggcaccagg actggccgtt gtgctggtgg gtagtaaccc tgcatcgcaa atttatgtcg
      361 caagcaaacg caaggcttgt gaagaagtcg ggttcgtctc ccgctcttat gacctcccgg
      421 aaaccaccag cgaagcggag ctgttggagc ttatcgatgc gctgaatgcc gacaacacca
      481 tcgatggcat tctggttcaa ctgccgttac cggcgggtat tgataacgtc aaagtgctgg
      541 aacgtattca cccggacaaa gacgtagacg gtttccatcc ttataacgtc ggtcgcctgt
      601 gccagcgcgc gccgcgtctg cgcccctgca cccctcgcgg catcgtcacg ctgcttgagc
      661 gttacaacat tgataccttc ggcctcaacg ccgtggtgat tggcgcatcg aatatcgtcg
      721 gtcgtccaat gagcatggaa ctgttgctcg caggttgcac cactaccgtg actcaccgct
      781 tcactaaaaa tctgcgtcac cacgtagaaa atgccgatct actgatcgtt gccgtcggca
      841 agccgggctt tattcctggt gactggatca aagaaggcgc aattgtgatt gatgtcggca
      901 tcaaccgtct ggaaaatggc aaagttgtgg gcgacgtcgt gtttgaagac gcggctaaac
      961 gcgcctcata cattacgcct gttccgggcg gcgttggccc gatgacggtt gccacgctga
     1021 ttgaaaacac gctacaggcg tgcgttgaat atcatgatcc acagggtgag taacatggcg
     1081 acattttctt taggtaaaca tccgcacgtt gagctgtgcg acttgctgaa actggaaggc
     1141 tggagcgaaa gcggcgcgca ggcgaaaatc gcgattgccg aaggccaggt gaaagccgac
     1201 ggcgcggttg aaacgcgcaa acgctgcaaa atcgtcgccg gtcagacagt gagttttgca
     1261 ggtcacagcg tacaggttgt tgcctgatcc ctttcccggc ctgtatcaac aggccggttc
     1321 tcttttcaga cttttgccct ctacgccccg ttcatccatg ctgatagcat cgacattttg
     1381 ccgaagcgac catcatgcca accgttatta cgcacgccgc cgttccccta tgcattggtt
     1441 tagggttgag ttcgaaagtc atccctccgc gtctgttatt tgcctgaatc atcctggcga
     1501 tgctgcccga cgccgacgta ttgtcgttta aatttggcgt tgcttacggc aatgtttttg
     1561 gtcatcgcgg gtttacccat tcgctggtgt ttgcgtttgt tgtcccgctg ttatgtgtgc
     1621 tgattggacg ccgatggttc agggctgggc tgattcgctg ctggctgttt ttaaccgtct
     1681 cgttgctatc gcacagcatg ctggattcgg taaccactgg cggtaagggt gttggctggc
     1741 tgtggccgtg gtcagatgaa cgctttttcg ctccctggca ggtgattaaa gtcgcgccgt
     1801 ttgcattgtc ttgttacacc acgccgtacg ggcatcaggt gattatttct gaattgatgt
     1861 gggtgtggtt gccggggatg ctactgatgg gaatgttgtg gtggcgcaga cgataaccgg
     1921 atgcgaaaac tcgcatccgg caatagcgca attacttacg acgccaggtg gtcccttgcg
     1981 ggccatcttc cagaacgatc cccatctcgt taagacggtc acgcgccgca tctgccgccg
     2041 cccagtcttt cgctttacgg gcatccagac gctgttgaat taacgcttca atctcagcca
     2101 cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc ttccggttct tgctccagca
     2161 ggcctaatac agcggaaagt ttacgcaggt gagatgccat tgcattcgcc gctgccatat
     2221 cttctgcttt cagacggttt acttcacgcg ccatatcaaa cagtacggaa taggcttccg
     2281 gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc ttcaaacgct tcgccaccgg
     2341 caggagcaac ggttttttct gtgccgcgca gggcagtgta gagacgctcc agcgccgcac
     2401 gcgcctgctt caggttctct tcgctgtagt tcagctggct gcgatagtgg cccgacatca
     2461 ggaagtaacg cacggtttca gcatcgtagt atttcagcac atcgcgcacg gtaaagaagt
     2521 tacccagcga tttggacatc ttctcgcggt caaccatcac cattccggag tgcatccagt
     2581 agttaacata ctgaccatca tgggcacagg tggactgcgc gatttcattc tcgtggtgcg
     2641 ggaacatcag gtcagaaccg ccgccgtgga tatcaaagtg gttacccagc tgcttgcagt
     2701 tcattgccga acattcaatg tgccagcctg gacggcccgc gccccacgga gacggccagc
     2761 tcggttcgcc ctctttcgac atcttccaca gaacgaagtc catcgggttg cgtttgtcgt
     2821 cgaccacgtc aacgcgcgcg cctgcctgca gctggtcgag atcctgacgc gacagcacac
     2881 cataagtcgg atcggtcggg acgtcgaaca tcacgtcgcc gttgtccgcc gcataagcgt
     2941 gacctttggc gatcagttgt tcagtgagtt caataatttc tgcgatatgg tgcgtcgcgc
     3001 gcggctccat atccgggcgc agaatgttca aagcatcaaa atctttgtgc atttcggcga
     3061 tcatgcgatc caccagcgcc acaaagcttt cgccattttc attggcacgt ttgatgattt
     3121 tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt atagccgagg aaacgcagat
     3181 agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg accaatatga cagagatcgt
     3241 aaacggtgat tccacacaca tacatgccga cttccccggc gtgaataggc ttaaattcct
     3301 ctttttggcg tgtcagagta ttgaagattt ttagcatcga agattccgtt tagacatgtg
     3361 tgggtaattg agttgcgtat aatacccata tttcccgcgc gaatcagcat acattgcgtg
     3421 atgatccgat cgtgcggtta tgctataaca ccaccctata tatgacccga actgggttga
     3481 agcaccaatc aaacggaaca ggatgcaaaa atggttactt tccacaccaa tcacggcgat
     3541 attgtcatca aaacttttga cgataaagca cctgaaacag ttaaaaactt cctggactac
     3601 tgccgcgaag gtttttacaa caacaccatt ttccaccgtg ttatcaacgg ctttatgatt
     3661 cagggcggcg gttttgaacc gggcatgaaa caaaaagcca ccaaagaacc gatcaaaaac
     3721 gaagccaaca acggtctgaa aaatacccgt ggtacgctgg caatggcccg tactcaggct
     3781 ccgcactctg caactgcaca gttcttcatc aacgtggttg ataacgactt cctgaacttc
     3841 tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg ctgaagtggt tgaaggcatg
     3901 gacgtggtag acaaaatcaa aggtgttgca accggtcgta gcggtatgca ccaggacgtg
     3961 ccaaaagaag acgttatcat tgaaagcgtg accgttagcg agtaatcgtg gcgacactct
     4021 ttattgcaga tcttcatctc tgcgtggaag aaccggcgat caccgccggt tttctgcgtt
     4081 ttttagcggg ggaagctcgc aaggccgacg cgctgtatat tcttggcgat ctgtttgaag
     4141 cgtggattgg cgacgacgag cccaatccac tccatcgcca gatggcggca gcgatcaaag
     4201 cggtgtccga ttctggcgtt ccctgttatt tcattcatgg caaccgtgat tttctgctcg
     4261 gcaaacgctt tgcccgtgaa agtggcatga cgttattgcc ggaagaaaag gtgctcgaac
     4321 tttatggccg ccgggtgttg attatgcatg gcgacacgct gtgcaccgat gacgcgggtt
     4381 atcaggcttt tcgcgccaaa gtccacaaac cctggctgca aatgctattc ctcgccctgc
     4441 cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgagc gaacagtaaa gaagccaaca
     4501 gcagtaaatc gctggcgatc atggacgtta accaaaacgc ggtagtcagt gcgatggaaa
     4561 aacatcaggt gcaatggctg atccacggac atacccatcg cccggcggta catgaactta
     4621 tcgccaacca gcaacctgct tttcgcgtgg tactcggtgc ctggcatacg gaaggttcga
     4681 tggtgaaagt cacggcggat gacgttgagc tgattcattt tccgttttaa aaaacccgca
     4741 actttgctga tttcacagcc acgcaaccgt tttccttgct ctctttccgt gctattctct
     4801 gtgccctcta aagccgagag ttgtgcacca caggagtttt aagacgcatg tcttcccgca
     4861 ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag cgactgggct accatgcagt
     4921 tcgccgccga aatcttcgaa atcctgaatg tcctgcacca cgttgaagtg gtttctgctc
     4981 accgcacccc cgataaactg ttcagcttcg ccgaaagcgc cgaagagaac ggttatcagg
     5041 tgattattgc gggcgcaggt ggcgcagcgc acctgccagg catgattgcc gccaaaacgc
     5101 tggtgccggt gctgggcgtg ccagtacaga gcgccgcact gagcggtgtc gatagcctct
     5161 actccatcgt acaaatgccg cgcggcattc cggtgggtac gctggcgatt ggtaaagctg
     5221 gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc gactcacgat aaagagctac
     5281 accagcgtct gaatgactgg cgcaaagcac aaactgatga agtgctggaa aacccggacc
     5341 cgcgaggtac ggcatgaaac aggtttgcgt cctcggtaac gggcagttag gccgtatgct
     5401 gcgtcaggca ggcgaaccgt taggcattgc tgtctggcca gtcgggctgg acgctgaacc
     5461 ggcggcggtg ccttttcaac aaagcgtgat taccgctgag atcgaacgct ggccggaaac
     5521 cgcattaacc cgcgagctgg cgcgccatcc ggcctttgtg aaccgcgatg tgttcccgat
     5581 tattgctgac cgtctgactc agaagcagct tttcgataag ctccacctgc cgaccgcgcc
     5641 gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg tttgatcgtt taggtgaact
     5701 ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc ggtcaatggc gtttacgcgc
     5761 caatgaaacc gaacagttac cggcagagtg ttacggcgaa tgtattgtcg agcagggcat
     5821 taacttctct ggtgaagtgt cgctggttgg cgcgcgcggc tttgatggca gcaccgtgtt
     5881 ttatacgctg acgcataacc tgcatcagga cggtattttg cgcaccagcg tcgcttttcc
     5941 gcaggccaac gcacagcagc aggcgcaagc cgaagagatg ctgtcggcga ttatgcagga
     6001 gctgggctat gtgggcgtga tggcgatgga gtgttttgtc accccgcaag gtctgctgat
     6061 caacgaactg gctccgcgtg tgcataacag cggtcactgg acacaaaatg gtgccagcat
     6121 cagccagttt gagctgcatc tgcgggcgat taccgatctg ccgttaccgc aaccagtagt
     6181 gaataatccg tcggtgatga tcaatctgat tggtagcgat gtgaattatg actggctgaa
     6241 attgccgctg gtgcatctgc actggtacga caaagaagtc cgtccggggc gtaaagtggg
     6301 gcatctgaat ttgaccgaca gcgacacatc gcgtctgact gcgacgctgg aagccttaat
     6361 cccgctgctg ccgccagagt atgccagcgg cgtgatttgg gcgcagagta agttcagtta
     6421 agcaggtgct catttgattg ccggatgcgg cgtaaacgcc ttatccggct taccgctccg
     6481 gtacccattg taggcctgat aagacgcgtc aagcgtcgca tcaggcatgg tgcaccgatt
     6541 gccggatgct ctcatcacta cagcgaaata caggtccccg cttcgcccgc cagcgtctct
     6601 tcaattcgcg ataacgcccc aatccacgcg ggtttaccac ggcttctgac ataaccactc
     6661 accgccgtta ccttcggccc catcgaacca tcggctttgg caaatggcgc taactcatcc
     6721 ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc agttttcata taccgcatca
     6781 gcatcggtga ggatcaccag tccatcggca ttaatctgct cggcgagcaa cgcagcggcg
     6841 agatctttat caatcactgc ttcactccct gccccatctt ccgtcacagg cacacctccg
     6901 ccgccactgc aaatcaccac atgcccctct ttgagcaaca actcgatggc ttcgctgtcg
     6961 agaattttac gcggttgcgg agacgccacc acccggcgca aatatttacc atcacgtttc
     7021 atctgccagc cgtaagccgc ttccagtgcc tcttgttctt ctggctgata aaccggacca
     7081 ataaatttct ctggctgcaa aaacgccgga tcatcaggcg aaacctcaat gcgcgtcagc
     7141 accgtc
//
LOCUS       DAEEBB010000182         6852 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEEBB010000182 DAEEBB010000000
VERSION     DAEEBB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.182"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="KLF07_003780"
     CDS             complement(<1..156)
                     /gene="cas6e"
                     /locus_tag="KLF07_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBD9833686.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="KLF07_003781"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="KLF07_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBD9833687.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="KLF07_003782"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="KLF07_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBD9833688.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="KLF07_003783"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="KLF07_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBD9833689.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4056)
                     /gene="casA"
                     /locus_tag="KLF07_003784"
                     /pseudo
     CDS             complement(2495..4056)
                     /gene="casA"
                     /locus_tag="KLF07_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4154..6811)
                     /locus_tag="KLF07_003785"
     CDS             complement(4154..6811)
                     /locus_tag="KLF07_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBD9833690.1"
                     /translation="MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCF
                     GLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGWPPDRMDELD
                     NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGS
                     STRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQ
                     QKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWL
                     THVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLICTQ
                     VLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQ
                     DPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1662 a         1863 c         1621 g         1706 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt tattgagcag cagcgggaaa tgatgttcgt accaacagcg
     2941 cgctttcatg ttgtcgaaat catagccaaa tccccacagg cccacttttg cctgcttgag
     3001 actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt tttctgactt
     3061 acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt gtggtttgac
     3121 cgagtaaaac tcgccgccct ctttaagtag tacacggtaa ggcgttaagg gatgctgcca
     3181 catggcatag ttagcgccgt aatttttggt cgtcatcagg ctcagaagcg cgtcgctctg
     3241 ctcaccgcaa atatcgcagt tgccgactgt ggtggtatta aaatcaatac gaatacggcg
     3301 aggcattccc cagtacgcct ggagtttatt gacctgctca tcggttacca ccgcaccggc
     3361 cagttcgctg atgcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa attttttggg
     3421 tagcggtaaa tcggcttcat cctgcggcat gacattgatc cacagtttgc gccacaaggg
     3481 agtttgttga ttaccctgat attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc
     3541 gcgtaaaccg gtgcggtagc ctttgccacc tgacggcgca ttcaactgta gggagaacag
     3601 agctaacgca gagcaatgtg ggcacaggta ttccgttaca ccacgcttga taaagtggtc
     3661 tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag cgaccggaac
     3721 tttatctccg gtgagcgcct cgaaatcctg tataaatgaa ggggaatctg gcccaaactg
     3781 gaaagcatgt tctaatgaca gcaatgcttc ccgtagcttt tcagcttcca gtccgtcttc
     3841 ccagatatca tcccaacgac gatgatcttt tggcgcgaag ctggtttgta gtagccccag
     3901 caaaaactgc cataccgcac cctggagatc tgcccgcggc gcagaaatat cgacaacatt
     3961 ttcatccgcc agatcgactg gcgccagttt gcctgtcgtt ccgtctttaa aacgaacggg
     4021 caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc
     4081 atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt cagagagtcc
     4141 tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa tacccacaac
     4201 tttcgtcatc gttgactaaa ataacgtttg ccatctccgg atcttgtcgc tgttcaatgc
     4261 accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt tttttccacc
     4321 agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg gcataaggct
     4381 tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca gccagacgtg
     4441 tcgacagttt ttctgggagg taatcgttga tattggcggc atagccgggc ctgaagttca
     4501 gcacaaactt tttagccatt gcgcgatcac agtaatattt gcccacttgc tcctgctcgc
     4561 tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca ataagaagcc
     4621 gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc gtcagccaga
     4681 ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca aaccattcgt
     4741 cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg gaacgctcgt
     4801 cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt aatcgtcctg
     4861 ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc aggtcacaat
     4921 cgacactctg ctctaatacc tgagtacaaa taaggacttt tccggcacgc tgtgagctat
     4981 cttctttacc aaagcgtgcc agtgtctccg tttcaattcg ctggcgatcg ctaaaagcaa
     5041 agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca agcagctgac
     5101 gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat ttcccctgac
     5161 ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa cccacgctga
     5221 cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga
     5281 catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc aggccctctg
     5341 taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac gtggcagaaa
     5401 gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca agaatgcacg
     5461 acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta ctcagcccca
     5521 gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga tccagcgtgc
     5581 cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag gccgcacatc
     5641 cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca gaccagagtg
     5701 attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg ctggggcggg
     5761 attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg gcattggccg
     5821 ttgccattgt tggcagacca aaaaacagcc cctgagcctt ccccgcagct atcaacctgt
     5881 gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc agaataaata
     5941 actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac ggtgtcggat
     6001 tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt gcttttagag
     6061 gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt ctgccagtaa cctttaatat
     6121 ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag tctgcgagca
     6181 cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc tcatcatcat
     6241 cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg agaaaatctc
     6301 gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggcc
     6361 aaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg
     6421 gagagagcga agatgaagga agtaactcct gacattcact taaataatga ttccacaacc
     6481 agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct
     6541 tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc
     6601 agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc cctaattgac
     6661 gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc atcgcggcta
     6721 catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc
     6781 gggtttttcc ccagaaatca aaaaaagtca caatattttt atccttcagt aaacttaaag
     6841 gatatttacg ca
//
LOCUS       DAEEBB010000183         6646 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEEBB010000183 DAEEBB010000000
VERSION     DAEEBB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.183"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..737)
                     /locus_tag="KLF07_003786"
     CDS             complement(<1..737)
                     /locus_tag="KLF07_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBD9833691.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="KLF07_003787"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="KLF07_003787"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBD9833692.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="KLF07_003788"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="KLF07_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBD9833693.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="KLF07_003789"
     CDS             4397..4678
                     /locus_tag="KLF07_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBD9833694.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4920..5462
                     /locus_tag="KLF07_003790"
     CDS             4920..5462
                     /locus_tag="KLF07_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9833695.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5542..6216
                     /locus_tag="KLF07_003791"
     CDS             5542..6216
                     /locus_tag="KLF07_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD9833696.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6232..>6646
                     /locus_tag="KLF07_003792"
     CDS             6232..>6646
                     /locus_tag="KLF07_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD9833697.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1754 a         1507 c         1594 g         1791 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 agatgcgata aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat
     4801 ttgtttcaaa taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat
     4861 ttgtttccaa gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag gaacatatta
     4921 tgaaacgttc aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat
     4981 ttgctgcaga cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt
     5041 gtaaattcga agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg
     5101 tctttgaagg taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca
     5161 tgttaaaaat tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg
     5221 ataaaataac cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc
     5281 tctatttagg tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt
     5341 ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag
     5401 ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt
     5461 aataaataaa attgtaagtt tctccaggta ataaacatgg ccgctatccc atggcggcct
     5521 tttaatttaa gaggcattaa aatgaaagga ttattatctt tactcatttt ttctatggtc
     5581 cttcctgcac atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat
     5641 aaagaagtga tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg
     5701 attgatgatg gcgatacgtc attaccacca gaaaaaattc aggttccttt catgttaacg
     5761 ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg
     5821 aataaactgc ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca
     5881 aatagtccag agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa cagaattaag
     5941 ttgttttacc gaccagcggg tattgctccg gtaaataaag cgacatttaa aaaattgctg
     6001 gtaaatcgca gtggcaatgg tttggtgata aaaaatgact cagctaattg ggtgacgatt
     6061 tcggatgtca aagctaataa tgtcaaagtc aattatgaaa ctattatgat tgccccctta
     6121 gaaagtcaga gtgttaatgt caaaagtaat aatgcaaata actggcatct gaccattatc
     6181 gatgaccatg gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga
     6241 atgaccccac ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct
     6301 gaagaaacct ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat
     6361 attcgtcttg atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat
     6421 aagcaatggc gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta
     6481 tcaagagaag ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa
     6541 tgtttaacat ttgagcaact tgttcagggt gggagctata cctgggatat cggggttttt
     6601 cgtctcgatt tcagtgtccc gcaggcctgg gtggaagaac tggaaa
//
LOCUS       DAEEBB010000184         6537 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEEBB010000184 DAEEBB010000000
VERSION     DAEEBB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.184"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="KLF07_003793"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="KLF07_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBD9833698.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="KLF07_003794"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="KLF07_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBD9833699.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="KLF07_003795"
     CDS             2408..3157
                     /locus_tag="KLF07_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBD9833700.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="KLF07_003796"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="KLF07_003796"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="KLF07_003797"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="KLF07_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBD9833701.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="KLF07_003798"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="KLF07_003798"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBD9833702.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="KLF07_003799"
     CDS             6018..6491
                     /locus_tag="KLF07_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBD9833703.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAEEBB010000185         6530 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEEBB010000185 DAEEBB010000000
VERSION     DAEEBB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.185"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..906)
                     /locus_tag="KLF07_003800"
     CDS             complement(<1..906)
                     /locus_tag="KLF07_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HBD9833704.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="KLF07_003801"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="KLF07_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBD9833705.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="KLF07_003802"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="KLF07_003802"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBD9833706.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="KLF07_003803"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="KLF07_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBD9833707.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="KLF07_003804"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="KLF07_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBD9833708.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6530
                     /locus_tag="KLF07_003805"
                     /pseudo
     CDS             6387..6530
                     /locus_tag="KLF07_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1685 a         1685 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
//
LOCUS       DAEEBB010000186         6515 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEEBB010000186 DAEEBB010000000
VERSION     DAEEBB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.186"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            38..250
                     /locus_tag="KLF07_003806"
     CDS             38..250
                     /locus_tag="KLF07_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833709.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="KLF07_003807"
     CDS             283..501
                     /locus_tag="KLF07_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBD9833710.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="KLF07_003808"
     CDS             656..889
                     /locus_tag="KLF07_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD9833711.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="KLF07_003809"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="KLF07_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBD9833712.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="KLF07_003810"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="KLF07_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="KLF07_003811"
     CDS             1917..2660
                     /locus_tag="KLF07_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBD9833713.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="KLF07_003812"
     CDS             2690..3229
                     /locus_tag="KLF07_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBD9833714.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="KLF07_003813"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="KLF07_003813"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBD9833715.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="KLF07_003814"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="KLF07_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBD9833716.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="KLF07_003815"
     CDS             4582..6102
                     /locus_tag="KLF07_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9833717.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="KLF07_003816"
     CDS             complement(6135..>6515)
                     /locus_tag="KLF07_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833718.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAEEBB010000187         6360 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEEBB010000187 DAEEBB010000000
VERSION     DAEEBB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.187"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(443..519)
                     /locus_tag="KLF07_003817"
     tRNA            complement(443..519)
                     /locus_tag="KLF07_003817"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Arg,seq:tct)
     gene            complement(533..609)
                     /locus_tag="KLF07_003818"
     tRNA            complement(533..609)
                     /locus_tag="KLF07_003818"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:Arg,seq:tcg)
     gene            complement(617..692)
                     /locus_tag="KLF07_003819"
     tRNA            complement(617..692)
                     /locus_tag="KLF07_003819"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Ile,seq:cat)
     gene            complement(733..1791)
                     /locus_tag="KLF07_003820"
     CDS             complement(733..1791)
                     /locus_tag="KLF07_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBD9833719.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1899..2139)
                     /locus_tag="KLF07_003821"
                     /pseudo
     CDS             complement(1899..2139)
                     /locus_tag="KLF07_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2364..3185)
                     /locus_tag="KLF07_003822"
     CDS             complement(2364..3185)
                     /locus_tag="KLF07_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD9833720.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3182..3562)
                     /locus_tag="KLF07_003823"
     CDS             complement(3182..3562)
                     /locus_tag="KLF07_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD9833721.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(3563..4549)
                     /locus_tag="KLF07_003824"
     CDS             complement(3563..4549)
                     /locus_tag="KLF07_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD9833722.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(4623..4895)
                     /locus_tag="KLF07_003825"
     CDS             complement(4623..4895)
                     /locus_tag="KLF07_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833723.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            complement(5199..5354)
                     /gene="hokD"
                     /locus_tag="KLF07_003826"
     CDS             complement(5199..5354)
                     /gene="hokD"
                     /locus_tag="KLF07_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD9833724.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5646..5879)
                     /locus_tag="KLF07_003827"
     CDS             complement(5646..5879)
                     /locus_tag="KLF07_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833725.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            complement(5987..6154)
                     /locus_tag="KLF07_003828"
     CDS             complement(5987..6154)
                     /locus_tag="KLF07_003828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833726.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            complement(6165..>6360)
                     /locus_tag="KLF07_003829"
     CDS             complement(6165..>6360)
                     /locus_tag="KLF07_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833727.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
BASE COUNT         1486 a         1788 c         1430 g         1656 t
ORIGIN      
        1 atagcggtca tgtgatgccg gtttcccggt aactcagcac cggtatctga gtcaacgttt
       61 tctctactgg gtcatttccg atacgccctg cctgctgaca ggctttcatc acatctgaaa
      121 atatagcacc ctgactgata ctgtagtacc taaggttcca gaaactgtga tgtatccggc
      181 acagaaaagc ccctccggag agggctggag agtggcgcta tgtgccattg catgatgccg
      241 ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact
      301 cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc
      361 gcaaaacaga caactgtcaa ccgtctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa
      421 agcgagccag ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc
      481 ttagaaggca attgctctgt ccggctgagc taacaacgca tgatgcagat aatggaccgc
      541 catcggggac ttgaaccccg cgcagccagc ttcgaaggct ggcgctcttt cctgatgagc
      601 taatggcggt atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
      661 gtcgctcgct ctcaccactg agctaaaggg ccgggagccg cataataacg acgcgtaatt
      721 aattcttcaa tatcatccgt tctggctgac taaatcctgt acttcccgaa ccgtctgctc
      781 aaaacgttca gtctccagct caacgccaat tgcacgacgc ccgagcgcca gtgccgcttt
      841 cactgtcgaa cccaacccca tgaaaaaatc tgcaatcagg tcacccggac gactgctagc
      901 gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc cgggatagaa
      961 ctgcaccggt ttatgcgtcc acacatcggt atacggcacc tgcaccgtca cgccaaaata
     1021 ccgccgcaga tgtttatatt cactcagcag ctccgcatac tgccggttca gtgaggtata
     1081 cgtatccacc agctggtggt ggagcttttc cagttcacca cgccgatgct tctcttctgc
     1141 cacccgggca aacagcgcct gtaatttcag ataatcgctt tcattcggca actgccactg
     1201 accggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatctg caatctgttt
     1261 tgccgttatc cccagggcag ctcgcgcatc acgaaagtaa gaaatcagcg gggccatcac
     1321 atgctgtttc agtgcactgc ccttcgccgc atacccggca tctttcggac gatacggccc
     1381 ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc gcaggctttc
     1441 cttgttgcat ccgttccagc gtccggacgg cttcgcccag ataatatggt tcagcacact
     1501 gaagcgttca cgcatcatga tttcgatatc agatgccagg cgatgaccac agaacaggta
     1561 aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt ccagccactt
     1621 caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca ctttaaagta
     1681 cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga taaattccag
     1741 gcagtcggcg ttgattaact cacaactgga tatttttaca gtattaagca tggatcatta
     1801 agcctgtctc tgataggctc attctgcttt tgcgcaaagc agtgggcctg aggtttgctt
     1861 gtgaacccaa cgcatgagca gatggctggt gggtgcccct aacacccacc agccgcccat
     1921 ttaccacaaa taaaaaagcc ttcactgcgg aaggcgtctg taacaaccga actgatagtc
     1981 tgccagaccc gccataacca gctgggtcag tattaactgg cagcgttcgc gtgaaaggta
     2041 agtattctgc gctatctccc cgactgtcgc cggttcggta acgcttaatt cattaaacac
     2101 cactctggcg gtttctgtca tatcctgctg ttttagcatg tcttttcctt ttccggttaa
     2161 cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagacag gtattcaccg
     2221 ccaccagcgc gtttactgta ctgacgcgat ttcagtcata aaaaacccgc tcggcggcgg
     2281 gttgtagaaa ctcttctaac gtcaggcata aaacgcccat cgttatgacg aatttaccac
     2341 agattccgga aaaatcaacc ttgttaccta gttacctttt ttaactgccg ctcagcccat
     2401 gcttcttcaa tatcaaaccg ggtcaccagc gcatcataaa atttcttaac tgttttttcc
     2461 catgacgcgc gtgttatctg gtttgtcacc tcgcatatag cattaaatgc ctccgttgat
     2521 ggtagtcttt catagccacg accaccacaa cgctggcagt ctctgataac aggcatacca
     2581 cgttttaccg actcttcacg atgaatggcg acaccacgcc cacggcaatc cttacaggcg
     2641 gtggaaacct caccctttcc gccacactcc ggacaggcaa cttttaccac ctccctgact
     2701 tttttccatt cttcccagta agacggatac acacctttcg tacactttgc ccataccggc
     2761 ggcttaccat ccggatactg gatcttgttt gtaaaaacct cgctttcaat aaattttttt
     2821 ccgtgacagc aggggcactg ttttttgctc gccgcgctac gggcataatc ttcaaacgca
     2881 tacgaagcca taatacgcat cactgccggt tttatttctg ccgggagttt tcttaacgcc
     2941 gccacgcgat cacaccgact gagtgcatat tctgtcagca attctgttgc ccgctctctg
     3001 tcattcatac taatgcccat tttcccaagg aacgcagaaa cccccatctc agcccgattc
     3061 tgtgtcatgc cctgcgcggc catcacatca gtgatactca gcgcatcttt cgacgttgag
     3121 gccgatgcat cagtcaggcc gggggatttt ggggagtagt atttcggtaa atcttccagt
     3181 ttcatttttt gacctgccct tcaagcatta tgggataaat cttcaccccc agacgtccac
     3241 cagatactgg ctgagcacga acgatattga tttcatcaaa ctgctcatcg tccattagca
     3301 accccgcatg cgtcagcgca tccagcggcg ctttcagaat attgtccagg tcacggcggc
     3361 gcttatccgg tggttctgca ataattttta ttgccaacct tccggacagg cttaatttca
     3421 gccgctgctg gcgaacaata agcgccactg cccggcgata acgctccccg gcttttgata
     3481 caaaatatgt gctgccacga cgacgccagt aagtgttcac cgtcggcggg taaggtaaaa
     3541 caaattctat gcgctcggtc atttatgctt tccacttcag aacacccgaa tttctcgcgt
     3601 gcattaaaaa acgaatcagc aacaacagct gactgccgtg tttctcttca aaatctttta
     3661 ctccggcatg tagttcgtta cggcatttac ggcataacgg aataacaaac aaatcatcag
     3721 cctttgttcc catccctccc agtccatgac caatgatgtg atgcggatca tctgcctgat
     3781 tgccacacgt catgcatttc tgcgttttta cccagcgcgt gtatacaggc atctcttccc
     3841 gttgtgattt ctggcgctgg agatactgag ccggtgactc cggatcaacg gcaatgctga
     3901 ccaccgtctt ttcctgtggt gggttctgtt gctggtggac gtgaggcagt aacgcaatat
     3961 tttttgtgcg ctgcttcagt atgctggtgg ctgtctgctc tcccggtatg atgtcgcttt
     4021 cactgtacac agagcgaatt ttttccgcac gcaaccccag cgaacgacgt aataccgctt
     4081 ccggtagcgc gtccgccacc tgattgcgga ccgcccacca ggataattca gccagagata
     4141 attcacgctc ctgcgtaccg cttattgcgt gaccgatgac gtcaatcatc catgctgaca
     4201 ggttttgatg agcaagttgc tcgagtgatt cggatgtctg gtcacgcagc tggttgtcgc
     4261 agtgccagca caacaccatt gcgccggtac cataacggtg aatgacggtt tcgctgtgat
     4321 gataatcgcc gtgtggccac tggcaggatt taatatggcg caacagccag tcagacaatg
     4381 caccagcacc accagcagca cgaatcaccc gtgcgttact gaaaaacggc agcaatgttt
     4441 tgtcttccac cagcggctgg cgagcggcag gaacgacccc ggacggcaga ttacgcatgc
     4501 tttttggttc cggctccacc agtattctgc cgttatggaa tgctgacatt gattcacggc
     4561 ctggcttaac gataaccaga ccgagttccg gtaccagaac aggtcgaagt aatacccgca
     4621 cattacctcc agatccgttg ctggaatgtg cgggacggac gcgttggccg ttcggagtaa
     4681 gggagcctga cggagattat ccagtgacgg tagtcgaggc taagggcttt tttaacctcg
     4741 catccgcgcc tgcggtaaca ctgaatgagc cattcggcct gttcttcagt gcatggggga
     4801 tgctggtacc aatcagattt aaatgcgtga gagcgccgcc cgtgcctgct ggcaaagacg
     4861 gctgaattat cagaattgtg tagtctggaa ggcagtggct gacaatagcg ccattctcaa
     4921 cgcgcacagt atagaggcca tcttcactaa aaatttcacg taattcttcg attttcatca
     4981 acagaatcct tccagataaa tagcactccc ctgttcgggg tccatccctc ttctccctgc
     5041 gcgctactta agtatttttg attctattcc ggcaccatct aaaacttcaa acgcgttgaa
     5101 aataaaaaca aaaacccgcc gaagcgggtt aagtgtgggt gcattgagga tgcctgaccc
     5161 atcagaggtg gcgagggatt tctcccccgc ctggtctctt actcctcagg ttcgtaagct
     5221 gtgaagacag cgacctccgt ctggccggtt cgaattcgta cctcgcagag gtctttcctc
     5281 gttaccagtg ccgtcactat gacggttaaa cagatgacga tcagggcgat taacatcgcc
     5341 ttttgctgct tcatagcctg cttctccttg cctttcggca cgtaagaggc taacctagat
     5401 ttgccgttca tagattgagc ctcagattaa tgttaagcgt cttgccggac gcgtaatgtt
     5461 aactggggct tttctctatc tgccttttgg tgttcatgcc tgagacagat agcctcaagc
     5521 acccgctgca attctactta actctccttt tcccgcaaac cgtttttatc cccagcggca
     5581 aatcgaatac accaccagcg ccaccgccat cgcaattcct accgttgtta atgcttcagg
     5641 ccaggtcatc gtaaaatatc ctccacgctt atcagtccgt tccgctccag ataactcatc
     5701 gccttatccg gtaatttgca gtctggcttc gctttcctca gttgccaggt taactgcttt
     5761 accagcatgg ttaactcatc gaccagacgc tgatatccca ctggtttgta ttcatgcaat
     5821 ttaccggctg gctctgctgc cagcgatacc agtgcgattt ccagaacagc aatatccatc
     5881 ttatatgtgc ggatgatgtc atggtcgatt gtgcccggta tgcacagtct ctgtgcttca
     5941 atagtctcct ctgcgtgagc tattaactgc tctctggtaa aagtcgtcat gccgtagccc
     6001 cttcttgata tttttcaaac cagaacacaa ccggctctgc ttccagcgat gccagcgcaa
     6061 tccgtgccag ttccatttgt tcaccacggg taagcccgtt ttcaagcggg tttttaatga
     6121 acaattcaat acgttctttg gtaatagtgg tcatgtgtta ctccttaacc cgcagtgctt
     6181 tcaactgatg aggggaacaa aatcttttca tcaaaccctg cattcatatc atgaacagca
     6241 acacaccaat ccatcgacga acgattatca agagcctcca tgatttcatc catgcggcgt
     6301 aggtcataca ggtaaatgct tttatcgcca atggtgtaaa agccaatttt tttcggtgat
//
LOCUS       DAEEBB010000188         6326 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEEBB010000188 DAEEBB010000000
VERSION     DAEEBB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.188"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..157
                     /locus_tag="KLF07_003830"
     CDS             <1..157
                     /locus_tag="KLF07_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBD9833728.1"
                     /translation="FINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAV
                     SGKRFER"
     gene            complement(510..1250)
                     /gene="phoN2"
                     /locus_tag="KLF07_003831"
     CDS             complement(510..1250)
                     /gene="phoN2"
                     /locus_tag="KLF07_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBD9833729.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(1579..2445)
                     /gene="ospB"
                     /locus_tag="KLF07_003832"
     CDS             complement(1579..2445)
                     /gene="ospB"
                     /locus_tag="KLF07_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBD9833730.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            complement(2695..2814)
                     /locus_tag="KLF07_003833"
                     /pseudo
     CDS             complement(2695..2814)
                     /locus_tag="KLF07_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3058..3430
                     /locus_tag="KLF07_003834"
                     /pseudo
     CDS             3058..3430
                     /locus_tag="KLF07_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3992..4939)
                     /gene="icsP"
                     /locus_tag="KLF07_003835"
     CDS             complement(3992..4939)
                     /gene="icsP"
                     /locus_tag="KLF07_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBD9833731.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT         1904 a         1246 c         1189 g         1987 t
ORIGIN      
        1 ttttatcaat aatggattgg ttgatgtaaa caaaaaattt caaaaagcaa atagtgggga
       61 cactatgctt gacaatgcaa tgaaaagcaa agattcaaaa atgattgatt ttttattaaa
      121 aaatggagcg gtatcaggca aacgatttga gaggtgacct aaaatatatt taggttgtgc
      181 cctgaatctg aagttgtcat ttctgttata ttgatcgaca taggggatgg tgcagaccgt
      241 tcccctgcaa caccacattg catgtggtga acggaaccgc cccgagtttc ctagagaatg
      301 ttttatctgt gaactcaggt tgccagatca tcgtttccga tggcagcata ataagctttt
      361 tctgcttctg ccggagggat atgtcccagc ctttccagta atcccttaaa ttcaggggta
      421 aaacgtggtg tgcccataga ctcctcctat gctcatatag ggtagatttg tctatgggcc
      481 ctccatagcc tgatacaggc tgtccagctt tatggggtca gttcattggt aggagtattt
      541 aattcttcaa actctttttt ggcttcgcta aggcttttgg taaattcagg tgtattatga
      601 agtactgcaa caaccgatgc tcccattaaa cgcccagcct ctacatcgct ttgccaatgc
      661 gcaccgcaga tgacccgact ttctccaaac tcatatccac gtcgaagtat ttccgcttta
      721 cgttgaggat taatctccgc aagtatcagt gctactgccc aaccaaagga tgcatgacca
      781 gagggataag agccagtgat agccatgttc tcatctttat caggtgtaca ggttgcgtct
      841 ttataaataa cgaatggacg aactctttta taatattctt tggcgttacg cacggcgtaa
      901 tcatggctat cctgtagtac ttgtgttaac aactgatata agattggggt attttccctt
      961 gaaatatcca tgccataagc atctgaaaat gcaagaccaa aattctcgta gtatgcatct
     1021 tcgctagcga gacgtacacg attagcatcc cggagcgagc ggccgaattc ataatgagct
     1081 ttgtcagcct gaaatactac tgaatcctct gccggaggcg gcggaagtat tgacaaactg
     1141 tctggtgaag tttgttgagt gagaaaacct tctgccttca gagcatttgc tgaggggata
     1201 aaaatcatat ttgtagcaat acaaaaaaga agaaagtttt tggttttcat aatttatttc
     1261 cttagcataa ttaattgatg attttggtca gctgtaaaaa acgagctgtg ttacaaaaaa
     1321 cacaagtcat gcggaaaaat ttgaacagag tgatggaaaa acaaaaaata gtcttatatt
     1381 aaagtaataa cattattttt catattcacg aaaaataatg tggtaaaaga atgatgtttt
     1441 ttatctattt gttcgtatcg ttttgtctct gtattatgat gatttgtgat tttccctcat
     1501 agggaagcac ttttctacta aagataaaat aacttcatct taaatgcaaa caattaacta
     1561 taggttaaaa attttatact aatccagttc tttattaata ataaaagtct ttttctgaga
     1621 gcttcttttt actgtatgct ccggatcagc aggaattgaa gttgaacgat aatgtgagta
     1681 gggaaaaagc tcttgaccat agtgaactcc atatccatga tagccggata tttttagacc
     1741 atcactcaat gactcatcgt tttcaaggtg tttttttatc tgctctagca aagattcttt
     1801 ttccttaaaa aaaggcagag agttaaggat acaagaaaga ctttcagcag gggcattgtt
     1861 aaaattttta ggagccactt tatcagcgga gccgcatgaa gtaaaacgga tatctttcac
     1921 atttagaata ccacagtctt tcattcttga aatgattgtt tgtacatcaa taagttccga
     1981 accaaggcct aattgatgag aaccaggact accgtgccca agaatataaa ccttattata
     2041 ctctttaaaa tcagtatcgt ttactgtttt tattttcgct ttttttacag aaaaataagc
     2101 agcagcattc aatgcgtcat catcattatt tattactatt ggttcactct gtggtagcca
     2161 tgattcagcc atttttagaa aatcattttt acttttttca tttttaaaaa cataatcata
     2221 taacatcttg ttcttaaata ctcttggtat ttgttgtccc ataatagata gaacatcatt
     2281 gctatcacaa aaacctttct ctcctaaaaa aaccaatgct gcttttgggt gagtacatga
     2341 tgaatttttt acccttttta ttatatgtgc aaatgcaata tctgatatgc tttcattctt
     2401 tttattgact attctacagt atggtctaac accatctaaa ttcataccta catgactcca
     2461 tatattgttt tataaacatc atagtaaagg gtgttatatt ttcatatagt atgcaagctt
     2521 ctttgtatac tatgatttaa aaaaacggaa caaaaatgca attatattct ggcattgttt
     2581 aatgctgttt gtctttttta ttcattattt caatgaaaga atttcaacta caaagttaat
     2641 tgatcctacc tacataatat agacacagta cctaaagtac cgtggagatc atccggggaa
     2701 gccccgggtc tgataccagt gcaggtagac cgctatcttg ttccggtaat gttctgccgt
     2761 tctttacggc atcacctcta gcagaaacat cgcgtaactg cgccaagtat gatgaacttc
     2821 tcttaccgct gcgatctggt tcaacaatat gcagatggta cacaacctct ttaaccagcc
     2881 catccggatt attcagagcc gcttccgcaa tccggaataa aagtgctgtt ttaccgtgaa
     2941 ccctctgaaa atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac
     3001 ggtggacaat ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg
     3061 ctgggagcgg tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg
     3121 acggataatg gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga
     3181 ctttgttgaa ccgaagaaca cggcggtgcg gagtccggag agtaacagaa tagcaaagag
     3241 cttcgtgaaa acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac
     3301 ggcagcaaag aaccttgcag aggcattcga gcattataac gaatagcatc cgcatagtgc
     3361 gctggattat cgctcgccac gggaatatct gcggcagcgg gccaatgata acaggtgtct
     3421 ggaaatatag gggcaaagcc acccacgaac gatccagcat ataatcaaga aattagattt
     3481 catttaacat aagatagatt atgtgcacca agaggcgttt ccttaatttc tgaatttaca
     3541 ggctcttttg ctctctttgc tatgctgtgg atttggaaag atagagtgct ggctgattat
     3601 ttaggtattt tcatcagcat gtccctgata gcactgttcc atcagcgctt tcaggcgata
     3661 atagtagacc ttgggtagcc tctgcccatg tgtaacttga taccccgaga acagccacga
     3721 tgatctaagc ggttcttatt tctccggtct cagggtcggt aatgccaaca gtaagtccac
     3781 agtaatcaac gaagagtttt ttgaaccgcc ccggaaatcc tggagactaa acgccctgag
     3841 aaagaggtaa acagaatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca
     3901 gttcgtatgg ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttttagc
     3961 catgaaaacg taatcaactc ggtgaaccaa ttcaaaaaat atactttata cctgcggaag
     4021 tgagaaaacc aatatattca atacttgcac tatttttaat ggtgccagat atattggtac
     4081 tttgctcata aagagatgta tcaccttttt tattagaaat gtaatttctg gagccttcta
     4141 tataaaattt tgcactgggg gtgacatagt atccaatatt cgcagcaaca gagcagtaat
     4201 tttggttttt tatttcatct ttaaaaattg tctcagtctg atagtgttta tctgtatcag
     4261 atgaacgaac ccaaccacta tatttcagtt ctgctccaaa ctcaaaattc tcatggcggt
     4321 aattagcagt taatccaata taaggtattt tgaagagctg tttgtaatct attgttcttt
     4381 caccactagg atgtgcccct tttttattcc tgttcccccc gttttcacta taaatataac
     4441 tccctcccat tgcattaaaa ctgtaacgac tctcttggta acctgctatt agtccaagtc
     4501 gataatccaa attatttaat aaccaacctt taagattcag ctcgaattcg ttagcatcac
     4561 ggagatgcgt attgggatgc cagctctggt cggtccatac ctgaggggta ttggaattgt
     4621 tccagtcttt atcaaccatg cttgctttct ggttacctaa agtagtccaa ccggaaactc
     4681 cgaaagacac ttttggaatt agcttccaat cgatgccacc tctaacaata gtcgcattac
     4741 tgtatttcca gtccagttga ctaatttttc tccccccttc cttgggatga taaacgcgtt
     4801 cttttgtttt gccactcaga gatcctagac taatatccgt actgatgttg tccggtatga
     4861 aaagtggata gttagtggta gcatgtgtag taaagatcgc tggtacacaa agtgcaagta
     4921 caaggaattt taatttcatc gagaactcta cttttttggt tgaaatgtcc atgatcttct
     4981 tactttataa gataaacgtc aatgccaagc attattttaa ttaaatattt gtttatgtta
     5041 ccaagaacat atcttttatt ataatattta ttgaactttt gtaaaaataa aaaaaatacc
     5101 tcattgtgcg aataaagtaa cgggggccat agatttctct acctaccttt tggtgttcat
     5161 acctgaggct gatagtttca ggattccgcg acaattctac tcaccttctt gttctctcgc
     5221 caatatgaaa tcaatcagaa aagcgttcca taagaacaac gataagacaa taaattgtca
     5281 tcacggctgc aatagccaac gtgcctttca gtaccaacat gacaacctcc tgtatttgac
     5341 gtaacgtgtg ctgataaata tgtatttgta tctcaactga ctcaatacaa cacttaagta
     5401 tagtttttca gattttgttt ttcccgtgaa atattatgga aatggcagaa gcaatacatt
     5461 gaattccgat tcgagtgaat acggctagcg ggcacctcac tttagcactg aagccatcaa
     5521 cttttatttg acttatttca atggaaagct ttctcactat cagtacaggt agttcccatc
     5581 cttttaaagt cgagagtcag actgcgacta atacagttaa agtgaccaaa ctcagttatc
     5641 ctctacacat tttacctcat gtttacttgg acaattcatt ggtgctttta taaaagaata
     5701 aatattctac taatttaatg catcacctaa aatagtattt tttataatag aactgtatct
     5761 atagtatatt attaaagctt aattatttat tatgctttaa tgtggttgtc cgattaagga
     5821 ctaatccttg aaaacgccgt atagggcggg agttcactct attgcgataa cgtaaccgta
     5881 caatattgtg cgtttcccaa attgaccact actgaactat tctgggattt aaatgcaata
     5941 aaatggttgg ttgaaggtcg tggtgatgat gttagtcctt ttgagaatat tccagtgcca
     6001 gatcttactt ataataaaga ggcttggcag tttggataag ctattgtcct tattccacta
     6061 atacaaactt tttgtaaagt atattaaata tacttcattt catactgaaa tccccggaag
     6121 tattcaatca aacaattaca ccataaaagg aagagggagc cccaattaag ttatcaagca
     6181 tgtttatgat acctgcaggg tataaatttt ctggctgcaa gcagacacat ataaaacgtc
     6241 tttattgatc ctacccacat aatatggacg cagtcgtaag tgaggctctg tgagcctacc
     6301 cacgtaatat ggacacaggc ctaagc
//
LOCUS       DAEEBB010000189         6325 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEEBB010000189 DAEEBB010000000
VERSION     DAEEBB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.189"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..355
                     /gene="mcbR"
                     /locus_tag="KLF07_003836"
     CDS             <1..355
                     /gene="mcbR"
                     /locus_tag="KLF07_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBD9833732.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(391..2493)
                     /locus_tag="KLF07_003837"
     CDS             complement(391..2493)
                     /locus_tag="KLF07_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBD9833733.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2735..3796
                     /locus_tag="KLF07_003838"
     CDS             2735..3796
                     /locus_tag="KLF07_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBD9833734.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="KLF07_003839"
     CDS             complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="KLF07_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBD9833735.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5677..6294
                     /locus_tag="KLF07_003840"
     CDS             5677..6294
                     /locus_tag="KLF07_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBD9833736.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1514 t
ORIGIN      
        1 caccccgcaa gacctgacgg aactccagga attgctcgag aaattacagc aggcgcaaga
       61 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
      121 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatggggcc
      181 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
      241 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
      301 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
      361 atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa tattcatgcc
      421 gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact cattgacaat
      481 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
      541 taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat aagacggcgc
      601 ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
      661 ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
      721 tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
      781 aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat cccatgccag
      841 ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc caccgctact
      901 gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac taagcagccc
      961 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
     1021 aaagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg tcggcgtttc
     1081 aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
     1141 accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt tacccggagt
     1201 aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
     1261 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
     1321 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
     1381 gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc cggtagtgaa
     1441 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc cctggtaatg
     1501 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
     1561 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcttg
     1621 cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
     1681 gtcgtactgt tctgcacgag gcgcttgttg tggattagcc ttccattctg ctttggttag
     1741 cccacccggg tcatctgctt tgatatccac actattgaaa atcagactta atttgctggc
     1801 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
     1861 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat cgccaggctg
     1921 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
     1981 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
     2041 taccccacca gacgcgttgc catacagggc agagaagggg ccacgcagca cttccacatt
     2101 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
     2161 aataccgtcc acatacaggc gaataccgcg aataccgtaa gtggagcggg agccaaatcc
     2221 gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct gcaaaccagg
     2281 cacgtcggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tctcctcgcc
     2341 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
     2401 gacaatcatg gtctgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
     2461 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
     2521 acgccgttat ggtaataaaa ttgtaaattc atggaaaacg aaacggcacg atacgttaag
     2581 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
     2641 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
     2701 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
     2761 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
     2821 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
     2881 ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggcgg
     2941 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
     3001 caagccgttt ggtgtcacca tcaataacac gactcagaca ctgtggtttg gtaacactgt
     3061 aaacagcgcg gtcacggcga tagatgccaa aacgggcgag gtgaaaggcc gtctggtgct
     3121 ggatgatcgt aagcgtacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
     3181 cgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
     3241 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
     3301 tctggcgctt gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
     3361 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
     3421 agagcacttc tttatcaaca ttagccttga taccgctagg cagcgtgcat ttatcaccga
     3481 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
     3541 tgcagcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
     3601 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tagtgaaaac
     3661 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
     3721 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccag acgatgtgat
     3781 tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc ctgactttta
     3841 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
     3901 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
     3961 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
     4021 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
     4081 aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc caggaagtga
     4141 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta atcgctttac
     4201 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
     4261 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
     4321 catacacaac cagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
     4381 ccataaaact cggtgcggaa ccgcccatcg ccatcgagcg cagaatacgc ccggtgcagt
     4441 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
     4501 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
     4561 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
     4621 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
     4681 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
     4741 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
     4801 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
     4861 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
     4921 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
     4981 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
     5041 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
     5101 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
     5161 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
     5221 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
     5281 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
     5341 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
     5401 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
     5461 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
     5521 cggcgcagca tcctacccgt tatcgatata cgatgcaaaa catcccctta caatcctgaa
     5581 ggggattcat acaactgacg aaaaaatgac aaatactttt gctggttaac ctgtgtactg
     5641 tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta tggcgtaccc
     5701 ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc ttatcagttc
     5761 gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa aaccctgaac
     5821 ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac ggagacagcg
     5881 gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt tgggcgcgcc
     5941 gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta tccaacattc
     6001 actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt aaagaagaat
     6061 gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac cgctgaacct
     6121 tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat gcgcacatgg
     6181 gggtctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat tgctgatgct
     6241 gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat ttgagtggga
     6301 taccagagga ttatgttatt tatta
//
LOCUS       DAEEBB010000190         6305 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEEBB010000190 DAEEBB010000000
VERSION     DAEEBB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.190"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="KLF07_003841"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="KLF07_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HBD9833737.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="KLF07_003842"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="KLF07_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="KLF07_003843"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="KLF07_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="KLF07_003844"
     CDS             complement(5959..>6305)
                     /locus_tag="KLF07_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833738.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAEEBB010000191         6256 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEEBB010000191 DAEEBB010000000
VERSION     DAEEBB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.191"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            122..952
                     /locus_tag="KLF07_003845"
     CDS             122..952
                     /locus_tag="KLF07_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HBD9833739.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="KLF07_003846"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="KLF07_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBD9833740.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="KLF07_003847"
     CDS             complement(2081..3541)
                     /locus_tag="KLF07_003847"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBD9833741.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="KLF07_003848"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="KLF07_003848"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBD9833742.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6256
                     /gene="gntP"
                     /locus_tag="KLF07_003849"
     CDS             5146..>6256
                     /gene="gntP"
                     /locus_tag="KLF07_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBD9833743.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1588 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctg
//
LOCUS       DAEEBB010000192         6201 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEEBB010000192 DAEEBB010000000
VERSION     DAEEBB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.192"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            12..754
                     /locus_tag="KLF07_003850"
                     /pseudo
     CDS             12..754
                     /locus_tag="KLF07_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="KLF07_003851"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="KLF07_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="KLF07_003852"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="KLF07_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="KLF07_003853"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="KLF07_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBD9833744.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="KLF07_003854"
     tRNA            complement(2777..2851)
                     /locus_tag="KLF07_003854"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="KLF07_003855"
     CDS             complement(2927..3859)
                     /locus_tag="KLF07_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBD9833745.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="KLF07_003856"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="KLF07_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBD9833746.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..5425)
                     /locus_tag="KLF07_003857"
     CDS             complement(5090..5425)
                     /locus_tag="KLF07_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833747.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5383..5775)
                     /locus_tag="KLF07_003858"
     CDS             complement(5383..5775)
                     /locus_tag="KLF07_003858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833748.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5782..6158)
                     /locus_tag="KLF07_003859"
                     /pseudo
     CDS             complement(5782..6158)
                     /locus_tag="KLF07_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1369 c         1373 g         1913 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccc ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattctgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta c
//
LOCUS       DAEEBB010000193         6081 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEEBB010000193 DAEEBB010000000
VERSION     DAEEBB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.193"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="KLF07_003860"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="KLF07_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBD9833749.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="KLF07_003861"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="KLF07_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBD9833750.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="KLF07_003862"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="KLF07_003862"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBD9833751.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="KLF07_003863"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="KLF07_003863"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBD9833752.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..>6081)
                     /locus_tag="KLF07_003864"
     CDS             complement(5254..>6081)
                     /locus_tag="KLF07_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD9833753.1"
                     /translation="TPNDTHFPLAKAALEAGKHVVVDKPFTVTLSQARELDALAKSLG
                     RVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVRDRWREQGGPGSGI
                     WYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSYPQRRVILHGTMLA
                     AAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRDGVLTRVEGEERVE
                     ETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGIESAKHRATLCLA"
BASE COUNT         1393 a         1621 c         1550 g         1517 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg t
//
LOCUS       DAEEBB010000194         6036 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEEBB010000194 DAEEBB010000000
VERSION     DAEEBB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.194"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            527..1653
                     /gene="ltrA"
                     /locus_tag="KLF07_003865"
                     /pseudo
     CDS             527..1653
                     /gene="ltrA"
                     /locus_tag="KLF07_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1736..1915
                     /locus_tag="KLF07_003866"
                     /pseudo
     CDS             1736..1915
                     /locus_tag="KLF07_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1945..2427)
                     /locus_tag="KLF07_003867"
                     /pseudo
     CDS             complement(1945..2427)
                     /locus_tag="KLF07_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2683..3663)
                     /locus_tag="KLF07_003868"
     CDS             complement(2683..3663)
                     /locus_tag="KLF07_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBD9833754.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3663..4862)
                     /locus_tag="KLF07_003869"
     CDS             complement(3663..4862)
                     /locus_tag="KLF07_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD9833755.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5184..5537)
                     /locus_tag="KLF07_003870"
                     /pseudo
     CDS             complement(5184..5537)
                     /locus_tag="KLF07_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5867..>6036)
                     /locus_tag="KLF07_003871"
     CDS             complement(5867..>6036)
                     /locus_tag="KLF07_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBD9833756.1"
                     /translation="AWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT         1645 a         1390 c         1408 g         1593 t
ORIGIN      
        1 tcagggttta caggagcact gaccaaacga cccggtgctg cttcattcta aggaatgagg
       61 tggtgagcga ctcgggaaaa ggcagagatc tactgtcacg tggggttcgg gaagctgaac
      121 gaaagtaaac tgtccgatga agcatcgaaa tcttagatga tgtcaaaatg gggccgtaca
      181 gccgccccct gataagcgca gaagacacct gcttactgac tgcgcggcat ccggtgtgaa
      241 ggcagcagga tcccgggcca gaccatctca gggaacgtga gaacctgttt ccggcacgta
      301 tcgggaaaca ggggcggacc cattcgtagt agtgatgaaa gatctgtaat gggtctggag
      361 cgaagggatg gggatcacat tcattgcaga agacacaacc caaaagggga tgacgtaact
      421 gatgcaacga aaatcatttg aaataccaaa agcactggtc tgggcatcat atctggatgt
      481 gcgtcgcaac aagggagccc ccggatgcga cgggcagact ttgaaaatgt tcgaccagca
      541 acgtgatggc aatctataca agatctggaa ccggctatgt tcgggaacat ggtttccgcc
      601 accagtgctg gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc tggggatccc
      661 aacagtatcc gatcgaattg ctcagggagc gataaaactt ttcatggaag aaaagcttga
      721 cccgattttc catgctgatt catatggtta ccgaccaggc aaatcagctc atgacgctct
      781 gaaacaatgt gccatccggt gctggcgtta tagctggata ctggaagtgg acatcagtgc
      841 gtttttcgac catgtgagac atgatctggt gctcaaagct ctggaacacc acgggatgcc
      901 taaatgggtc atcctgtact gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa
      961 tggagaatta ataacccgaa cacgaggcac tccgcagggc ggggtcatta gtccgctact
     1021 tgctaacttg ttccatcact atgcatttga tttgtggatg gaaagagaat atcgggggta
     1081 ccgtttgaga ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca
     1141 aggctgaaga acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg
     1201 aaaacgaata tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc
     1261 tttctcggat atgacttcaa agtgcgtacg ctgaagaatt tcaaaggcga acggtaccga
     1321 aaatgcatgc cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag
     1381 tggcgtatac atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg
     1441 atagtgagag gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga
     1501 ctgtggagtg caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg
     1561 aaccggaagg ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt
     1621 cactggtatt tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc
     1681 acgtacggtt ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg
     1741 ttccgacagc gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct
     1801 gaacaatgtg gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggct
     1861 ggcaaaccgg gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc
     1921 aatacggttc tgacgagccg cttacttttg gcgtaaatct gctgcgcctt tactgaacac
     1981 aatgccgtat tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga
     2041 aaccaaccga cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg
     2101 tacgggtcgt atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc
     2161 atttttctgg caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg
     2221 gtcgagtttc ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac
     2281 agtcatttta ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt
     2341 tcctgcgggt ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc
     2401 aatgccggtg attttctgtt cagacatggt attccgctta cttttgtggt taaagtttat
     2461 ccatcatggc gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact
     2521 gttaaacggc taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat
     2581 ttcaccgtga aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag
     2641 ccaattaaaa actggtgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt
     2701 atcttttagt acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct
     2761 gccgaattca tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact
     2821 atcaaatttc cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga
     2881 agacatttcc ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt
     2941 gtcatcagca gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga
     3001 tatatcaccc agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt
     3061 cagctcagac tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag
     3121 ggcacgagtc accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat
     3181 accggcctct ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga
     3241 cgtctgcaaa tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac
     3301 aagtacacgt aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa
     3361 aatctcaata agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaatgtat
     3421 agttctggtg atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac
     3481 ctcttcaacc acaaaagttt gagtgtccac tttgtcatga tcaaccttga tctcagtaaa
     3541 ttttgccttt cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt
     3601 attattcagg atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc
     3661 catcagtagt tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct
     3721 gctgccatac gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca
     3781 gatatcacgg tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa
     3841 acatcaagca tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct
     3901 gacttattcg ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca
     3961 tcctgttcaa taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg
     4021 aaatcaactt gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt
     4081 ttcaaaaatg cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc
     4141 agcttatcaa taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc
     4201 tcgcacagag tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca
     4261 taaacgccag ggataactga cggaacgata aaatcttcca gtagttcctc acgactcacg
     4321 ttctgcaaca tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc
     4381 agaaacatag tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct
     4441 gacagcaggt gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta
     4501 gacacaccgc cttttagact accgatgaat atggaatatg cctcactata acgatcgcga
     4561 tatttaggaa tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat
     4621 tttgtcgcgg ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg
     4681 actgcaccat ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa
     4741 tagacttgat aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt
     4801 agggcgagca acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc
     4861 attccccctc cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg
     4921 taatgcatgc aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta
     4981 ttaatcatcg gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga
     5041 tttcccccga ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca
     5101 gatggtgtat ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca
     5161 acaggcgggg gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg
     5221 actttttcgt ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg
     5281 atgcgggtgc ggagggtccg gttgttacgt tcaatgcgtt gcgtgaagat tttgccggtc
     5341 aggtgtttct ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg
     5401 ccaccatgcc gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat
     5461 tccgtggccc gaaagtcgaa agtgtaagcc aacacaccgc cagttttagt gttgtacgca
     5521 taccagagcc agtgttgtgt caacgacgga tgaaaagtga tccacttata tctccaccaa
     5581 cggcccaata ttgatccacc gttttactca ggattagctt ctgctataac cccggccttt
     5641 cgtttctgtc tgagtcgata gctttctcct ttgatttgaa cgaaataatc cgacggtgaa
     5701 tcacgctgat attcgcgggt tggataccgc attacagagg tccgtcatat gagcctgcca
     5761 ctttcttacg tcgcgaatca gctttgttcc gccgtcgcca agcgctaacg tgcgttactc
     5821 ttgtaccggg ctacgtcgcc ttcagggaac tggttattct cattttttat ttgtgacatt
     5881 gtggttgttg tgcgacagac attgctattc ggcccatatc ccagataaaa cccgccagct
     5941 cacgtgcaac agcaacaatt gtaatattga cattctttcc tttggcctga agttttcgat
     6001 acctcttaca aagcctgagt tgagcatccc atgctc
//
LOCUS       DAEEBB010000195         6015 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEEBB010000195 DAEEBB010000000
VERSION     DAEEBB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.195"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(369..1304)
                     /locus_tag="KLF07_003872"
     CDS             complement(369..1304)
                     /locus_tag="KLF07_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD9833757.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="KLF07_003873"
     CDS             complement(1802..1945)
                     /locus_tag="KLF07_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HBD9833758.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="KLF07_003874"
     CDS             complement(2029..2286)
                     /locus_tag="KLF07_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HBD9833759.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="KLF07_003875"
     CDS             complement(2296..3933)
                     /locus_tag="KLF07_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HBD9833760.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="KLF07_003876"
     CDS             complement(4154..4687)
                     /locus_tag="KLF07_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833761.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="KLF07_003877"
     CDS             complement(4690..5616)
                     /locus_tag="KLF07_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBD9833762.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="KLF07_003878"
     CDS             5792..>6015
                     /locus_tag="KLF07_003878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833763.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAEEBB010000196         5988 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEEBB010000196 DAEEBB010000000
VERSION     DAEEBB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.196"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..143)
                     /locus_tag="KLF07_003879"
     CDS             complement(<1..143)
                     /locus_tag="KLF07_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833764.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAG"
     gene            complement(157..1605)
                     /gene="gabD"
                     /locus_tag="KLF07_003880"
     CDS             complement(157..1605)
                     /gene="gabD"
                     /locus_tag="KLF07_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBD9833765.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(1628..2896)
                     /gene="lhgO"
                     /locus_tag="KLF07_003881"
     CDS             complement(1628..2896)
                     /gene="lhgO"
                     /locus_tag="KLF07_003881"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBD9833766.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(2916..3893)
                     /gene="csiD"
                     /locus_tag="KLF07_003882"
     CDS             complement(2916..3893)
                     /gene="csiD"
                     /locus_tag="KLF07_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBD9833767.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            complement(4229..>5988)
                     /locus_tag="KLF07_003883"
     CDS             complement(4229..>5988)
                     /locus_tag="KLF07_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBD9833768.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
BASE COUNT         1422 a         1502 c         1477 g         1587 t
ORIGIN      
        1 cccgcgaaat caagatactc acggccttca acgtcccaca cccggcagtt ttccgcgcgg
       61 tcagcgaaaa tcgggtgaat ttgcccaacg ccacggggaa tcgcctgact gcggcgctgc
      121 attaactctt tattgctgtt cattcgcatt ctccagttaa agaccgatgc acatatattt
      181 gatttctaag taatcttcga tgccatactt cgaaccttca cgacccagac ccgaggcttt
      241 gatgccgccg aacggggcca cttcattgga aataatgccg gtattgatgc cgacgatgcc
      301 gtactccagc gcttcgccca cgcggaagac gcggcttaaa tcacgggcgt agaaataggc
      361 ggcaaggcca aactcggtgt cattggcttg cgcaatcaca tcggcttcat ctttaaagcg
      421 gaacagcggg gcgagggggc cgaacgtctc ttctttcgac actttggcgt tggccggcac
      481 gtccaccaga atggtcggct ggaagaagtt gccgccgcgt tcatccgctt taccgccgca
      541 aaccacacgc gcacctttct ccagcgcatc ggcaatatgc tcttccactt ttgctaccgc
      601 tttttcatcg atcagcggcc cgatggtgac gcctttatcc agcccgtcgc cgatgtgcag
      661 tttgctcacc gcctgctgca atttttcggc aaagcggtca tacacgccgt cctgcacgta
      721 taaacggttg gcgcagacgc aggtttgccc ggcgttgcgg aatttcgagg ccagcgcgcc
      781 ttccacggct ttatcgagat cggcatcgtc aaagacgata aacggcgcgt tgccgcccag
      841 ctccagcgac acttttttga tgtctttcgc gcactgttcc attaactggc ggccaatttc
      901 ggtcgaaccg gtaaacgaca gtttgcgtac cagcgggttg ctggtcagtt cattaccgac
      961 cgcgcctgcc gaaccggtga ctacgttaaa cacgccagcc ggaatgcccg cgcgaatcgc
     1021 cagctccgcc agcgccagcg cagagaacgg tgtctgactg gcgggcttca gcaccatggt
     1081 gcagcctgcc gccagcgccg gaccggcttt acgggtaatc atcgccgccg ggaagttcca
     1141 cggcgtgata gctgcggtaa cgccaatcgg ctgcttgata acaatcaggc gcttatcggc
     1201 ctgatgaccg ggaatggtat cgccataaat acgtttgcct tcttcggcaa accactcaat
     1261 aaaagaggca gcgtagctga tttcgccttt cgcttcggcc agtggtttgc cctgttcgag
     1321 ggtcatcagg cgcgctaaat cgtcctgatg ctccatcatc aaattgaacc agttgcgcag
     1381 aatgttggcg cgttctttgg cggtgagcgc acgccaggcg ggcagggcgc ggttggcggc
     1441 gtcgatagcg gcgcgggttt catcagcgcc cattttcggc acgctaccca gcttgtcgcc
     1501 gttcgccgga ttggtgacgt cgatgatccc gccattgttg gcgtccagcc attccccgtt
     1561 aatcaacgcc tgctggcgga ataagttact gtcgttaagt ttcattgtta cgtcctgttt
     1621 tcaaaggtta ttgattaaat gcggcgtgta aggcatccac actacgtgcc gctcgcagcg
     1681 tgcgtccggg gttactctgg cttgccaaca gcgtttgtac cttgctgaca atatgcgcac
     1741 caataggaat tgctgatgtc gctgccgggg agggcgcatt gcaggtgtgg atcgtgcgtg
     1801 gggtggtgac aaacagaaaa tcgtcaatca gcttgccgtc cggcgatact gcctgcgccc
     1861 gcacaccggc gggccagggc tggagatcgc ttaacgaaag ccggggacaa tacttttgca
     1921 ccagccgcag gtagccgctt ttgcacagcg agtttttcat ctcgcccagt cctgagcgta
     1981 gatggttttg cagcacccgg cgaatccccg acgagctcaa aatctccagc gtgtcactaa
     2041 atgagaagtc gcgcttgcga tagccttcgc gtttgaaagc cagcaccgcg tttggcccga
     2101 cggtcacgct gccgtcgatc atgcgggtga gatgcacgcc caaaaatggc atcgcggggt
     2161 cgggaatggg gtaaatcagg tggttaacaa tctggttatg ctccggcgca aggcggaaat
     2221 actcaccacg gaacgggcag atgataaagc ccggttcgag gccgagcatt ttcaccagcc
     2281 ggtcagccat cagcccggaa cagctaatca gcgtcgatgc ttcatattca ccgccctgac
     2341 gggtgcgtat caccacgccg tttttatgct tactgagggc gctgacttcg gcgttataaa
     2401 taatctcgcc gcctctggcc tggaagattt ttgccattgc cgctgtgact tcgcggtagc
     2461 tgacaatgcc gctggacggc acaaaaatgc caccaagccc ggtgatattc ggttcgcgct
     2521 cgcgcagttc cacggcgttt atccactcgc gctcgatacc gttcgccgct gtgcgttccc
     2581 ataaggcgcg catccgttcc atttcgagct gagacgtggc gaccagcatc ttgccgcagt
     2641 tgtcatagcg aatgccgttt tgatcgcaaa aggctttggt ggcgcggttc cccgccaggc
     2701 aaaactgcgc cttcaggctg ccgggcgtgt aatagacccc ggcatggatc acgccgctgt
     2761 tgtggcccgt ctggtgacag gccgggccgg actctttttc cagcaacgca atgcgggcat
     2821 ccggataaac atcaatcagt tgcatggcgg tcgacatgcc gatgatgccg ccgccaataa
     2881 tcacaaaatc atacatccgc tcaattcctt tgcgcttact gatgcgtctg gtagtggtga
     2941 gtggcgtaag cgaaatagcc acgctgacgc atcagttcac ggcgcagatc cgggtgcgga
     3001 gtaaagcggt cgcgaccgtg cagccagaac aggttgttta tcaacaggaa tttgccaacg
     3061 ggaacgggta cagaaagaat gcctttgctg gtttcaatgg catcggaaag ctcgctcaac
     3121 cacacgcctt cttcaaaatc ttttggctgg acgaactggt cgatatagcg catcaccggg
     3181 cgaccctgtt gatcaacgtc gaacactgga tggaaaacat ctttgctgac gtttttgctc
     3241 ggcggcgcgg caaagcgcat cgggcgacgc gccagtggat ggcggaaata gtggtccaga
     3301 tgttcccagt catcgagatg cagcagcaac gaatttccac cctgcatgtt ttgctcatcg
     3361 attttcatca tcagcacgta atcggtgatc tcttcgacat acgtgccgtc gttgtgcagt
     3421 tccatcacgc ggtgcggctg acgcagatag ctgtctgagt tatcgacatt tttcaccacg
     3481 aaacgcgcgt aatactgacc gctcatcgca tcgaaatttg agcgaccaat cagatgcgct
     3541 accgccgttg ccagcttcac catctcatct gcctgcgcca catcatcgat acccaccgca
     3601 ttgatcaaca gcgcaccttc agcacggttt agcagggtct tcaacagcaa tggttgcagc
     3661 tgattcgcac acagatcgtc aagaattttg cctacccgaa aacgcagaaa cgatttgtac
     3721 tccagcgcct gtacgggcca ttcggcaacc tgctcgagaa actgtttggt cgtctgttcg
     3781 gtgaaggtga gttccagcag acgcggggat tgcgccgacg ggatgagagt gaatccgcta
     3841 tagtcctggc ctgaatcgac agcgttattt tgtacggcgg tcagtgcatt catcagaagc
     3901 gatcctctta tgagatgtag ggtgacatgg cgatgctcat ttcgtagcca taatctaaaa
     3961 atatctacat ttctgaaaaa tgcgcacaaa agcgacatat tgttttctta ttgtgatcaa
     4021 aagcaacaaa ttagtaacat ttaacgtaga gtattaaggc aaagtgagac aggaaatgaa
     4081 taactaaagc aacaggttag ggggagttaa cattagagaa cagattatct ggtcgataaa
     4141 aaacagtaaa tggcttcact gcaaaatgac cgttagtaaa ctaaacatat tatctgcccc
     4201 gttttatcgg ggcagatata acattaaatt ataaatagag atagttcggc ggttctcctg
     4261 ttttccctaa aagaagagcg ctacgcccag gaatattgat atttagagtt ccgcctctaa
     4321 gcgtaaatac tttaccaggg gggataattt ttcgggtatt attaataata atgttaacgc
     4381 tattactccc catgttttta tatattccag gttgcaattt gttaggccag gtaccttcaa
     4441 cttcttcgtt tcctttattt attataacta gtccatcatg aggagagtga ttaatgttca
     4501 cagaatcccc acgactaaag gctagaacgt ttttagttgc aaatacaccc tcaaatgcgc
     4561 tccatattcc tgctttatct atatataatg cgtggaattc attgtagagc tgctgatagg
     4621 tagagcaacc tttattaagc cacgcctcac caccgacacg cgtggaccca tcataatttt
     4681 taatttcagt cgttgatttt aatacctggc catcccagta aactaaaggt ctgctgaacg
     4741 ggagagcggc catatatgcc attgctaatt gttcatcttg ttgatggtta aatagaaagt
     4801 cattgaaaag attgccattg ttaggaaaat cgtggttaat gttaatcaat atccgtcttt
     4861 ctgatgggat gcttgagatt gtggaacccg gttttgatgc aagggttagg agatctcccc
     4921 cgaatgaaaa ggcttttatc gtcttcatga gcaatgaaaa atcaatggcg cttaaatcat
     4981 ctctttcata aagcagtgat gacatttgtt gttcatccaa ctgacaagtt accagatatt
     5041 ccatgtaatt cataacggct tttttatgat attttgggtt aaataagtta gtattgtgta
     5101 accgctcatt ataattttta agcggtgggg ttattgatct ttctatttgt tcatgttttg
     5161 agtgttttgc cgcatcataa cgtaaacctc gtacgccata ttttcgcatt tcacgaacgt
     5221 attgattttg caacataata acctggtcac ttttgggtgt tgttttagga attccattta
     5281 aacgcttttc ttttaatgca tggaggtttt cccattcatc cttagagatg ttatctttac
     5341 ttgcttcgaa gtcttttgca gtgagatgta tatcttctgg atggatttgt ttacgttctt
     5401 ttgttactgg atctttaatg aatggaatca aaacctgacc ttgccaatag ccagggatac
     5461 caccttctat atctttactg atcttcgctc gtatatcagc agagggataa tccaggtcat
     5521 ttttaccact acccccaatt cccataaagt taaaggtata atcaggaatg atatcgatac
     5581 caaatttttt agcatcttta accaatgcaa taaaatcctg caaatctccc attggattat
     5641 ggatggtaca aagatcttcg ggttgataag catgaaacca gtaacccttt tcttcttcgg
     5701 gagtttctgg tccgtcggcg cggaaagttg tcactggagg gatttgtata tacccataat
     5761 ggcaacgtgc aaactcagac atattttccc tgatttgctg gtaggtggca ttaaaagcat
     5821 gaagaatcgc ttcgccgggt tttttagggg tgatgatttc acattgtgat ttctgaacag
     5881 cagtaagaga tttacgctca agtgtctgcc gtagatgttc tggattgatt agtattttaa
     5941 atgtttcttg ctgtccggaa ggtgacgtaa cgttaatatt atatacat
//
LOCUS       DAEEBB010000197         5961 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEEBB010000197 DAEEBB010000000
VERSION     DAEEBB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.197"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            292..368
                     /locus_tag="KLF07_003884"
     tRNA            292..368
                     /locus_tag="KLF07_003884"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="KLF07_003885"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="KLF07_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBD9833769.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="KLF07_003886"
     CDS             complement(1357..2247)
                     /locus_tag="KLF07_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBD9833770.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="KLF07_003887"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="KLF07_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBD9833771.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="KLF07_003888"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="KLF07_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAEEBB010000198         5930 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEEBB010000198 DAEEBB010000000
VERSION     DAEEBB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.198"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1095
                     /gene="gabT"
                     /locus_tag="KLF07_003889"
     CDS             <1..1095
                     /gene="gabT"
                     /locus_tag="KLF07_003889"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBD9833772.1"
                     /translation="VAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFAKKTL
                     LVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMGLMPG
                     HVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAASPAFM
                     QRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPLAGVT
                     GRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDGLLAI
                     AEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGPYYNV
                     LRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            1406..2806
                     /gene="gabP"
                     /locus_tag="KLF07_003890"
     CDS             1406..2806
                     /gene="gabP"
                     /locus_tag="KLF07_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBD9833773.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            2827..3489
                     /gene="csiR"
                     /locus_tag="KLF07_003891"
     CDS             2827..3489
                     /gene="csiR"
                     /locus_tag="KLF07_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBD9833774.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(3490..3939)
                     /gene="kbp"
                     /locus_tag="KLF07_003892"
     CDS             complement(3490..3939)
                     /gene="kbp"
                     /locus_tag="KLF07_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBD9833775.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(4023..4181)
                     /locus_tag="KLF07_003893"
     CDS             complement(4023..4181)
                     /locus_tag="KLF07_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBD9833776.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            4364..4663
                     /locus_tag="KLF07_003894"
     CDS             4364..4663
                     /locus_tag="KLF07_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9833777.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            4673..5197
                     /gene="ygaP"
                     /locus_tag="KLF07_003895"
     CDS             4673..5197
                     /gene="ygaP"
                     /locus_tag="KLF07_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBD9833778.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(5244..5648)
                     /gene="stpA"
                     /locus_tag="KLF07_003896"
     CDS             complement(5244..5648)
                     /gene="stpA"
                     /locus_tag="KLF07_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBD9833779.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         1391 a         1458 c         1561 g         1520 t
ORIGIN      
        1 gtggccgcag tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct
       61 tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc
      121 aagaaaacgc tgctggttac gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc
      181 cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg gcgcgtatca cgggcgcacg
      241 cattacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat ggggctgatg
      301 ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc acggcataag cgaggatgac
      361 gctatcgcca gcatccaccg gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc
      421 atcgtgattg agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg
      481 cagcgtttac gcgctctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag
      541 agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt ggcgccggat
      601 cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc
      661 gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg
      721 attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcatgaaaa tctgctgcaa
      781 aaagccaacg atctggggca gaagctaaaa gacggattgc tggcgatagc cgaaaaacac
      841 ccggagatcg gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat
      901 ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat
      961 aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtaccg
     1021 ctcaccattg aagacgctca gatccgtcag ggtctggaga tcatcagcca gtgttttgct
     1081 gaggcgaagc agtagctatg tccgtttgta agagttaacc gttgctaagg gaatgataac
     1141 gatgcagtaa aaaataataa cgatgaggcg aacgacaaag ttaaaaacga ctgcggacag
     1201 tcccctcgcc cctctggaga gagggttagg gtgaggggta aaacacgcgg ctaaaccctc
     1261 ccctggccct ctccctaagg gagagggaga aaaccctccc gctttcgttt ttaagatttt
     1321 gttgaccgtc tcaattaaac gcgtgacccg gcgcgggaaa tgtcggggcg ctctcccaag
     1381 tgacacactt tcgagaggat tcaggatggg gcaatcatcg caaccacatg agttaggcgg
     1441 cgggctgaaa tcacgccacg tcaccatgtt gtctattgcc ggtgttatcg gcgcaagtct
     1501 gtttgtcggt tccagcgtcg ccatcgccga agcgggcccg gcggtattac tggcctatct
     1561 gttcgccggg ctactggtgg ttatgattat gcggatgttg gcggaaatgg cggttgccac
     1621 gcccgatacc ggttcgtttt ccacctatgc cgataaagcc attggtcgct gggcgggcta
     1681 taccatcggc tggttgtact ggtggttttg ggtactggtt atcccgctgg aagccaatat
     1741 cgccgctatg atcctgcact cgtgggttcc aggcattccc atctggttat tttccctcgt
     1801 cattaccctt gccttaactg gcagtaattt attaagcgtt aaaaactacg gcgaatttga
     1861 gttctggctg gcgctgtgca aagtcatcgc tatcctggcc tttattttcc ttggtgcagt
     1921 cgcaattagc ggtttttacc cttatgccga agtgagcggg atctcaagat tgtgggatag
     1981 cggcggcttt atgcccaacg gtttcggtgc ggtattaagc gcgatgttga tcaccatgtt
     2041 ctcgtttatg ggcgcagaaa ttgtcaccat tgccgccgcg gaatccgaca cgccggaaaa
     2101 acatattgtc cgcgccacca actcggttat ctggcgtatt tctatcttct atttgtgctc
     2161 tatttttgtc gtcgtggcat taataccgtg gaatatgccg gggctgaaag ccgttggatc
     2221 ttatcgctcg gttctggaat tgctcaatat tccccatgcg aaattaatca tggactgtgt
     2281 gatattactt tccgtaacca gttgcctgaa ctcggccctg tataccgcct caaggatgct
     2341 ctactcctta agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca accgcagtaa
     2401 aaccccgtac gtggcggtgt tactctccac cggcgcggca ttcttaacgg tggtggtgaa
     2461 ctattacgcg cctgcgaagg tatttaaatt tctgatcgac agctccggcg ctatcgccct
     2521 gctggtttat ttagtcatcg ccgtttcaca gttgcggatg cgcaaaattc tgcgagcgga
     2581 aggaagcgaa attcgcttgc gaatgtggct ttatccctgg ctcacctggc tggtcatcgg
     2641 ctttattacc tttgtgttgg tagtgatgct attccgtccg gcgcaacagt tagaagtgat
     2701 ctccaccggc ttattagcga tagggattat ctgtaccgtg ccaattatgg ctcgctggaa
     2761 aaagctggta ttgtggcaaa aaacacccgt tcataatacg cgctgataat gatcaggagt
     2821 cacaccatga ccattacgtc tctggatggc tatcgctggc tgaagaacga tattattcgc
     2881 ggtaattttc aaccggatga aaaattacgg atgagtttgc tgacatcgcg ttatgcactt
     2941 ggcgttgggc cgttgcggga agcactttct caactggtgg cggaacggct ggtcacggtg
     3001 gtgaatcaaa aagggtatcg ggtggcgtct atgtcagagc aggagctgct cgatattttc
     3061 gatgcccgcg ccaatatgga agcgatgtta gtgagtctgg cgattgcccg cggtggcgat
     3121 gagtgggagg cagacgttct cgcaaaagcg catctgctca gtaagctgga agcctgtgac
     3181 gccagcgaga aaatgcttga tgagtgggat ctgcgtcatc aggcgtttca tacagcaatt
     3241 gtggcgggct gtggttctca ctatttgctg caaatgcgtg aacggttgtt tgatctggcg
     3301 gcgcgctatc gatttatctg gctgcggcga acggtgcttt cggtagaaat gctggaggat
     3361 aaacacgatc agcaccagac cctgactgcg gcggtactgg cgcgagatac cgcgcgcgcc
     3421 agtgagttaa tgcgccagca tttactgacg ccaattccca ttatccagca ggcgatggct
     3481 ggcaattaac tactcttccg gaatacgcaa cacttgcccc ggataaattt tatccgggct
     3541 ttttagcatc ggtttattcg cttcgaagat tttattgtac agattagcgt taccgtagac
     3601 ctgtttggaa atggcactca gagtgtcgcc agacttaacg gtataaaact ggctggcagt
     3661 ggctggtgtc gccgttttca cctgatcatc aacactggca ataccggaaa tattccccac
     3721 cgcaacaagg attttctcct tcgcctcctg actcaggccg tcaccagtga ccgtcgcttt
     3781 gccgtcggca atttgaatgt tcactttatc agcatcaggt ataccggttt tgctcagatg
     3841 ctcctgcacc ttcttcgcct gatcgtcttt atcgtgctga cctgtaaccg cgtcccagag
     3901 tttttctccg gcatctttca caaaattgaa cagacccata gctacctcat tcgttaacgg
     3961 aaacactcag aaagtgtagc agaggaacgc ggcgcgttaa cgccgcgggg gtgagcacag
     4021 gtttaatcgc gggtttgcac ccagaacgcg tgaatcaaac cggggatata cccaagcagt
     4081 gtcagcagaa tattaataat gaacgcccaa ccgaaccctt taccgagcag cacgccgagc
     4141 ggcggtagaa tgatggtgat gacgattctc cagaaaccca tatgtactcc ctataagaaa
     4201 attactcatt gtttaaaaag agattttatc tcttaagcgt agtaatttta gcggagcctg
     4261 ccagtttcag cttacgcttt tactctcatt tacgctgctg aggctggtat atgttgcatt
     4321 tttagtttag agcttactta atttagaaaa tacttaaata aatatgactg aactcgcgca
     4381 attacaggcc agtgccgaac aggcagcggc cttattgaaa gcaatgagcc atcctaaacg
     4441 attgctgatt ctgtgcatgc ttagcggttc ccccggcacc agcgcgggag agctgacgcg
     4501 cattaccgga ctgagtgcct ctgcgacatc acagcatctc gctcgtatgc gggacgaagg
     4561 gcttatcgac agccaacggg atgcccaacg cattctatat tccattaaaa atgaggcggt
     4621 aaatgccatt atcgccaccc tgaaaaatgt ctattgtccg taaggagtca ctatggcttt
     4681 gacaaccatt tcgccgcatg atgcacaaga attaatcgca cgcggcgcaa agttaatcga
     4741 tattcgtgac gctgatgaat atcttcgtga acatattcct gaagcagatc tggctccatt
     4801 atccgtgctg gaacagtcag gtcttccacc taaattacgt cgcgagcaaa ttatattcca
     4861 ctgccaggca ggtaaacgca ccagtaataa cgccgataaa ttagcagcga ttgccgcccc
     4921 cgcagaaata tttttactcg aagatgggat tgatggctgg aaaagagctg gattgccagt
     4981 agcggtaaat aaatctcaac ccttgccgtt aatgcgccag gtgcagatcg ctgcgggtgg
     5041 tttaatatta atcggcgttg tactgggtta taccgtaaat agcggtttct tcttattaag
     5101 tggctttgtt ggtgccgggt tactgtttgc aggaatcagc ggtttttgtg gaatggcaag
     5161 gttgttagat aagatgccgt ggaaccaacg agcttgagaa gcgacgccgg acgcgcccta
     5221 gcagcgacat ccggcctcag taattagatc aggaaatcgt cgagagattt accttctgcc
     5281 agcgcctgag caattggctt cggtgtacgg ccctgaccgg tccaggtttt agtttcaccg
     5341 ttaacatcgg tgaatttata tttcgccgga cgcggctggc gttttttacc agcgcgtggt
     5401 gcagcagcag agctattacc caataactct tccgggttaa ttccgtcagc cttcatcagc
     5461 tccagccagg tgctaatttt ttcctgacgc tcagccagtt cacgctgctg ctgttcttct
     5521 tcttcacgtc tttctttagt gacaaccctg aatttttcga gcatttcttc aagaacgtca
     5581 atggagaatt cgcgagccat cgcacggagg gtgcgaatgt tatttaaact ttgtaacatt
     5641 acggacataa taaagaaaac cttttaacgc caaaacaaaa aagtatttcg tcgcgtagtg
     5701 taagcgatca cttattatat ttccacaaaa tttaatctat ttacagtctt gcgcgagatt
     5761 atttcagctg ttattttctg aaactctcag aataatttca agattatata ttagccattt
     5821 tgatgagtaa tttagattat tggtgaacag aacggcgctc tctgctcgct aattccgcaa
     5881 gcctgacaaa accgaagtga ataaaaatgc aatataacag aataaaagaa
//
LOCUS       DAEEBB010000199         5824 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEEBB010000199 DAEEBB010000000
VERSION     DAEEBB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.199"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(139..3654)
                     /locus_tag="KLF07_003897"
     CDS             complement(139..3654)
                     /locus_tag="KLF07_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD9833780.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..4408)
                     /locus_tag="KLF07_003898"
                     /pseudo
     CDS             complement(4106..4408)
                     /locus_tag="KLF07_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(4682..5701)
                     /locus_tag="KLF07_003899"
     CDS             complement(4682..5701)
                     /locus_tag="KLF07_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HBD9833781.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         1442 a         1523 c         1219 g         1640 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acagtattta tatgaacgca tcccgtttgc aaggcatcca
     4441 ttgttttgac atgacgggta ggttgcagct ttatatccgg ctttattgca gccgtaacgc
     4501 tgcgagcccc tatgtaattc gccgactcac cccttatttt cacggcggac ttcaagccca
     4561 aaatactgtt caggtttagt gagtcacggt cttacctgtc tttgtcatca ctctggccgc
     4621 catagcaacc tttcagcatt cacccttttg ttaagagttc attgtttaat ggtatcagcg
     4681 attaatatgg tctgatactg acgtggttcc tgtcatactt acggtcatgg gcggttatcg
     4741 cccatactgt tcgtgccagc ttgtttgcca tggcgacgat tgccacactg gctggacgac
     4801 gttttttcag ttcggttatc cacgggccag gctctttagc tactaatgcc accgctcttg
     4861 caccgtggat aaataatgtc ctgagataag tgtcaccacg tttgcttatc cccagcagac
     4921 gtactttccc tccggagcca gtttgttttg gaaccagacc aacatatgcg gcgaactctc
     4981 gccccgattt aaacgctgat gcttccccca tggtggccac cgctgccgtc gcaataagtg
     5041 gtccaacgcc aggaatatcc agcaaccgct tacaggtttc attctgcctc gccacgctgg
     5101 taagctgttt ttcaatatcc tctatcagtg agtccagctc attcagtcgg ttgtactggt
     5161 tttccagaac cataatgaga tacggtggca gtctctcttt catccgttcc agtgcttcgg
     5221 ggaactcccg ctccatcgct gcccggcctt tgtggatggt ttcaccaaac tccagtaacg
     5281 tcccgtgcag ggcattaatt tgtgcggtcc ggaacttcac cagttgcatg cgggtacggt
     5341 gcagaaccag taccgactgc tgttcttctg tttttacggc gatttcttta cccggctgct
     5401 gaaccgccat ccagatagcc cgggcatcca tcacatcatt cttattgccc atgacgaatg
     5461 ccttaacgaa gcgggcctgc aacaaccgga ctttatgacc aagttttgtc agttcccgtg
     5521 cccagtgctg agaacctcca caggcctcca taccaatcag gcatggctca cggttgccga
     5581 agaacgtcag aaaatcctgt ctacgcaact gtttatcaac cacttcacct gtgtgctcat
     5641 tgatgaagtg aatctgaatg acatgttttg cgatatcaac gccaaccggt gtatatttca
     5701 tttgtggagt ctccagtctg agggagcttt atgcatcccg tattgggcac tatgatgccg
     5761 gaaatctgcg aggctccaca cctgccagtt tcaccctttt aaccatcata ctgctccgtg
     5821 ttta
//
LOCUS       DAEEBB010000200         5734 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEEBB010000200 DAEEBB010000000
VERSION     DAEEBB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.200"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..471)
                     /gene="ospC4"
                     /locus_tag="KLF07_003900"
                     /pseudo
     CDS             complement(1..471)
                     /gene="ospC4"
                     /locus_tag="KLF07_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
     gene            complement(705..2414)
                     /locus_tag="KLF07_003901"
     CDS             complement(705..2414)
                     /locus_tag="KLF07_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBD9833782.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2846..3565)
                     /locus_tag="KLF07_003902"
     CDS             complement(2846..3565)
                     /locus_tag="KLF07_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBD9833783.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPNYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3690..4253)
                     /locus_tag="KLF07_003903"
                     /pseudo
     CDS             complement(3690..4253)
                     /locus_tag="KLF07_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4237..4495
                     /locus_tag="KLF07_003904"
                     /pseudo
     CDS             4237..4495
                     /locus_tag="KLF07_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4604..5182)
                     /locus_tag="KLF07_003905"
     CDS             complement(4604..5182)
                     /locus_tag="KLF07_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833784.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5608..>5734)
                     /locus_tag="KLF07_003906"
     CDS             complement(5608..>5734)
                     /locus_tag="KLF07_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD9833785.1"
                     /translation="CYTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVENGQHQ"
BASE COUNT         1654 a         1196 c          970 g         1914 t
ORIGIN      
        1 tttatagttg gcaccatctt tactaagtaa gtattcaaca tcatacagat tacaatatct
       61 attgttgttc attttctcca tctctgcgac atcttgtttt gtaaaattaa atttagatag
      121 taaataaagt gctatatcag cttttttatt gcctaaagaa taagcaaggg cctgatgagc
      181 cattttttta tcagtcactt tattgttaat ctcttcataa ttagatgctt taatcgcatc
      241 ctccaaagat atgtctctga gttttatttt tttgaagtta tctgtactta gcattcttgg
      301 tatatgatac tcaagctgaa acacaaagtt tataattcta tccagactaa caggatcaat
      361 gtttttgtta taacaaaact ctcggaatcc ttgatcttta ctctttctta taaaatcaat
      421 aagatgcaac ccaattccaa gcctcatatc tttagtatta aatattggaa catactcacc
      481 ctctggatgt gacctttatc catgaagaag atttggccac accatggaaa cctgaacaat
      541 catcagttta cgcccatgta aacccctacg ggatatttga actcgatatg gaaataaggt
      601 tgctaatcga ggtggttgcc tgaaagttgg cagttacccg gtcactacac cttgttggag
      661 tgaacatatc aaaaacaggt aattacacaa tttaaattac agtctcataa aaaatgatta
      721 aatctattta ttatttcaat aaacgttttt ttcatcttct ttgaaaattg ttctgctata
      781 gtatccggtt ccagattgtt ctcttttatt attctacaat actctaatat tgtttctttg
      841 tctctattta ctacagatac aaatacacca ggaataccat ctttattagt tgcggacagc
      901 atttcgacca atcgtcttgt ggtaaaacta tagtttttta gaatattaca aaaggcacgt
      961 attgtttcgg tatggccatt ataaaatgcc ataaaaagtc ctgatgtacc gttgggattt
     1021 tttatccata ataattcatc tatatattcc tggtgagggg ggagtatttt tagtatatct
     1081 ccatacgcca taatggcctt tgtgtgccca ttttgaagtg ccataaacaa cccatgcttt
     1141 actttcaata attctttaat ttgttcacta ctaagcattt tcttattaag cagacttccg
     1201 aacatacgaa tactatctgc atgtccattc tgcaatgcaa aaaataaccc aggtaaatca
     1261 tggctgtctt tcgcagctaa tagatataat ctcttatcgg aagttaacgg ggttttaaag
     1321 actatttccc cataggcttt tattgtgtct acatgtccgt tcataaaaga catgcataaa
     1381 cccggcacat tgtttgcccc taccgcagca agcaatgtaa gtattgtttc ttgactaaga
     1441 ttgcacatat ttaatatctt accatatact tgaataatgt ctgaacatcc gttttgaagt
     1501 gctatataaa atgcaggagt accatcagag cttctcgtgg ccagaaggtt caccagttta
     1561 gattgagaaa tttcaccatt tttgtatttt tccatcagta taaataatac ttctgtcaga
     1621 ttctttgtta tcgcatgata tataactctg gaattgatca attcatctgg taaaagtttc
     1681 actgtgtttg ttagagggga attatgccta tcttcaaata ttacctgccc gggttgctcc
     1741 agaccataaa ttttccaata aaaatcatca aaaaaatcct tagcggataa ttgactcagt
     1801 tcgaaatggc tatcacaagt aaatacagta cgtttatgag taacagttcg attaggatcg
     1861 aaaaactgaa caacccattt ttcactttct tgtacgcgct ttattttcaa ccctaacgcc
     1921 atttggtggt ttaatgtgca tgctgataat gttctgacaa agtctccatt ttttttcatg
     1981 ttataaaaaa aattagtaat aactgttccc cactttttat tttccacaaa ataaatataa
     2041 gctggtgatt ttgaaaaatc ttccgttttt tccaaacata cattttttgc aatagcatca
     2101 ggggtgctaa gaagctgata gtttaccttc ccatttgttt ccataaaatt catttcccaa
     2161 taggaagcta ggtgacggca aactatttgt cttccatata tatcttttgc cactccattt
     2221 atatttacct tcacatttct cttttctgaa aaataaggtg ctgttttatt cttaaactga
     2281 attagttcac tggagtttga gaccctcata atagaacttg ttattgttct attatctctg
     2341 ttaacagaca tatctgttga taacgaattt tttgttgaaa atatactaga tgaaaatgtt
     2401 ttatttaacg gcatagaaga caaccataga gatgttataa tactattaga ttgcagttac
     2461 taatagtaaa agcctcctta caaggaggta gcttattcag aatgctccta taagatagcg
     2521 agctcgtcaa cgatacagat gatctgatta tattgtgcaa aaggagaaca ggaaaatagg
     2581 cactggaaaa gatgaaggcc tgatgggagc attaactaat gcaaaatccg acaggttaat
     2641 atcccgaaaa gcaatcaaac gactcaaaca ggagatgcac gctgaagtgg tggacaactg
     2701 ttcggatttc agatgagtag ctcagcagtt tatgagccga atgctctgtc acgggcagga
     2761 atcaggctgc atacgcagtg ccaaatctaa tcgtagttat tctaaccagt tttcaagcta
     2821 caaggtggtg tagctggcat cttctctact ctatcatcaa acgataaaat ggttcctctc
     2881 gtaacatttg ctcttgcctt tcacttccat ctcgatcgct tcgtagttca ttacgatagc
     2941 tgacatattt ccagtcttca ggtctaacat ctgacgccgg atactccccg ggagcgattt
     3001 tgcttcttaa tagattagac tcgagacaca ttataaattg tcgtatttta tgaagtaata
     3061 aagcactata ttgcgagcat tcctgatctg attttacata tagcgtaaac tgagctccta
     3121 tccccacacg agattgctga gaaactcgat tcatatcagt tatcttccat ttatctatag
     3181 gactgtcctc tgaaaataat aaaccagaca aaatttggaa tgccagagga acttgctccc
     3241 tagctatact aatatgaaac ttgtccccaa caaaatcccc cttactctga ggattttcac
     3301 gacatgcatg aataaacaca tctccactat ggttcacaat ctggaatcct tggtacatga
     3361 cagaaaaaaa ttgacatata tcagagtaat ttggcacgtt cgatgctact ttgaaatagt
     3421 acgctgggta ttgccgtatt tggttgtaca atatattgaa gttatttttc aatctttcat
     3481 tcgcagaaag catctgcggt atttcagacc tcacaacatt caaagaatct aaatttagtt
     3541 ttagacaggg cttttttatg ggcatagaaa acgtcctcta taaaatagat atattatctt
     3601 atattgattg tctatcatct tttataaaac gatacattat atgtaatgta tgtaagcgta
     3661 agggtcttct ccgatcatgg tgggaaggct tagaacgcca tattcagttt cccatagtgg
     3721 aacatcactc ccagcttaaa gcgctcccgg ttgcggtagc ccttggcttt tattctcagc
     3781 agcctgatct tgctgttcag tgcttccgca tttccgtttg atacgccatg gcgcatggcg
     3841 ttcaggatac cgtacaacct tttccctatc gttttagcca cattcttcat catggggacg
     3901 tcactgttgg ccgccagcga cagccatctc tgccagtcgt tgcgtctttc ctcgctccat
     3961 ggtctgctcc agatatcctt tgccagttct ttcagcgccc agcacaggct cgtcagcttc
     4021 atctgcgcac gcagccatat cagcttttcc tgcctggatt cggtcatcca tttattgctg
     4081 tgctgccaca ggaagcgagt tccctttgcc tgacgacgac tttcaacagg aagatgcgga
     4141 tgttcattct ggcgggtttt atccaccacc tcacccggtt gtttcgccac atgaaagcga
     4201 tcgaaggcta ttttctcaac cgcattgggt aagtggatgc gcgctgctct tagtttgctg
     4261 agccagttta gcattgcctt ctacatgacc gatgcctgag ccggtatacc gaactttatt
     4321 gtcctcaacc agtcatgtta taagcaagaa atgcactcag cggatagagc gacataatct
     4381 gaacctgcgg acacatctta agcggcttac ccgcaaaacc atatgcttct caaagtctga
     4441 tgatatgcac tacaagatca tcggctggta tctgacaatc aaccaccacc actaaatctg
     4501 cgtcacgacc tgcaactgag tctggttgaa caggctgagg ccctgctgaa gcaacgcgaa
     4561 cctctcgact cttgttggat tttaccaaat agtttttcta aaattaaaca catttatata
     4621 agttctttgc tgaacaaaac gcagcccgcc atcctttttc attcataaat tcagcaatgg
     4681 cagataaaac tttttcatca agcatataaa aaataatgcc agtagactca atatgcctca
     4741 tacaaataag gccgcacata tccgtaatat atttgtcaat gctgctataa tttgcatggt
     4801 aatatgccat gctattatct gatgatttta catatgcagt atgacatatt tttccttcac
     4861 gattaacaaa actgacaaaa ccactttcac caggagtaat atataaagat gaatttaacg
     4921 tgcgctcact aatattcatt aattgttgaa attcaatgga atagttttca gttaatgtat
     4981 ctattatctt ggctttgtct ttctttgtta tctcattgtc tttttcggca agaaaaattg
     5041 ccactttatg acatgcattc agctcgttaa ttttacctga taaaactgct tttcttacca
     5101 catcgttgaa ctcatcgata gtgtgttttt tattatatat atcatgaaaa atatagccta
     5161 aagacttaaa cgagactttc attttatttt atattctcct taaatattaa caggatatat
     5221 atatcacatt ttcctctata ttttcagaaa ccagctatca ttttccccat tactggtacg
     5281 tcgcttcaga tatttccgca gcaagcgata accctgcgca ctatgaggat gccattcatt
     5341 ataatgtttg aacaccttcg cgagactact ttactgctgt taacacgtca ggttcgaaca
     5401 taacactgat ttaatcatat ttcatcgttt tgacgaagtt ttctgccatt ccattttgca
     5461 ccgccgtgtg tttgggggca aggccgacca tctgtgtttc attcgccaga tataagtgat
     5521 gagatggcat tctccctcct tcacggcaga agcattttga ccgtttaggg gatgcggacg
     5581 attttcatgc ctgctaagat actttcgtta ctgatgctgt ccgttttcca ccatttcatc
     5641 atcactgatt ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg
     5701 gtaactctct cctttgatat tcagcgtgta gcag
//
LOCUS       DAEEBB010000201         5705 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEEBB010000201 DAEEBB010000000
VERSION     DAEEBB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.201"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..855
                     /locus_tag="KLF07_003907"
     CDS             <1..855
                     /locus_tag="KLF07_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBD9833786.1"
                     /translation="FGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPG
                     TKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAET
                     IGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVG
                     GIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEH
                     LELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQ
                     DTPDMREY"
     gene            923..4465
                     /locus_tag="KLF07_003908"
                     /pseudo
     CDS             923..4465
                     /locus_tag="KLF07_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5446..5705
                     /locus_tag="KLF07_003909"
                     /pseudo
     CDS             5446..5705
                     /locus_tag="KLF07_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1399 a         1399 c         1787 g         1120 t
ORIGIN      
        1 tttggcaacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
       61 gacccggacc agccgattat tatggggcgt acctaccacc aggaaaaccg cacacccggc
      121 agcctgccgg ggacgaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      181 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
      241 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgct
      301 gaaaccattg gcaacaatca gatgattgcg gttaccaaca atcagataga gacggtgggc
      361 gttaaccaga tagagacggt gggcagtaac cagataatta aagtgggttc tgttcaggtg
      421 gaaacgattg gacttgttcg tgcgctgacg gtgggcgtgg cgtatcagac gacggtaggt
      481 ggcattatga acacctcggt ggcactgatg cagtcctcgc agatcggttt gcataaatcg
      541 ttgagggtcg ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt
      601 aaaacgaaaa aggatgatac cgggcagacc gcgatttact ccgccggtga gcatctggag
      661 ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gacaaatttt tctcaacggc
      721 acaaaaattc atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg
      781 aactgtgcag cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat
      841 atgagagagt actgatcccg tacctgttcg gtattcagta acaaaccagc attaaaaata
      901 aacagaagaa ggaatttgaa tcatgagcgg aaaaccagcg gcgcgtcagg gagatatgac
      961 tcagtatggc ggtcccattg tccagggttc ggcaggtgta agaattggcg cgcccaccgg
     1021 cgtggcgtgc tcggtgtgtc cgggcgggat gacttcgggc aacccggtaa atccgctgct
     1081 gggggcgaag gtgctgcccg gcgagacgga ccttgcgctg cccggcccgc tgccgttcat
     1141 tctctcccgc acctacagca gctaccggac gaagacgcct gcaccggtgg gcgttttcgg
     1201 ccccggctgg aaagcgcctt ctgatatccg cttacagcta cgtgatgacg gactgatact
     1261 caacgacaac ggcgggcgga gcattcactt tgagccgctg ctgccggggg aggcggtgta
     1321 cagccgcagt gagtcaatgt ggctggtgcg cggtggtaag gcagcacagc cggacggcca
     1381 tacgctggcg cggctgtggg gggcgctgcg ccggatatcc ggttaagccc gcatctttac
     1441 ctggcgacca acagcgcaca ggggccgtgg tggatactgg ggggggctga gcgggtgccg
     1501 ggtgctgagg acgtactgcc agcgccgctg ccgccgtacc gggtgcttac cgggatggcg
     1561 gacgctgacg taccggcgtg aggccgccgg tgacctggcc ggggaaatca ccgacgtgac
     1621 ggacggtgcc gggcgggagt tccgtctggt gctgaccacg caggcgcagc gtgcggaaga
     1681 ggcccgcacc tcttcgctat cttcttctga cagttcccgc cctctctcag cctcagcgtt
     1741 ccccgacaca ctgcccggta ccgaatacgg ccccgacagg ggtatccgcc tttcggcggt
     1801 gtggttgatg cacgacccgg catacccgga gagcctgccc gctgcgccac tggtgcggta
     1861 cacgtatacg gaagccggtg aactgctggc ggtatatgac cgcagcaata cgcaggtgcg
     1921 cgctttcacg tatgacgcgc agcgcccggg ccggatggtg gcgcaccgtt acgcgggaag
     1981 gccggagatg cgctaccgct acgacgatac ggggcgggtg gtggagcaac tgaacccggc
     2041 agggttaagc taccgctatc tttatgagca ggaccgcatc accgtcaccg acagcctgaa
     2101 ccggcgtgag gtgctgcata cagaaggcgg ggccgggctg aaacgggtgg tgaaaaaaga
     2161 actggcggac ggcagcgtca cgcgcagcgg gtatgacgcg gcaggaaggc tcacggcgca
     2221 gacggacgcg gcgggacggc ggacagagta cggtctgaat gtggtgtccg gcgatatcac
     2281 ggacatcacc acaccggacg ggcgggagac gaaattttac tataacgacg ggaaccagct
     2341 gacggcggtg gtgtacccgg acgggctgga gagccgccgg gaatatgatg aaccgggcag
     2401 gctggtatcg gagacatcgc gcagcgggga gacagtacgc taccgctacg atgacgcgca
     2461 cagtgagtta ccggcgacga caacggatgc gacgggcagc acccggcaga tgacgtggag
     2521 ccgctacggg cagttgctgg cgttcactga ctgctcgggc taccagaccc gctatgaata
     2581 cgaccgcttc ggccagatga cggcggttca ccgcgaggaa ggtatcagcc tttaccgcca
     2641 ctatgacaac cgtggccggt taacctcggt gaaagacgca cagggccgtg aaacgcagta
     2701 tgagtacaac gccgcaggcg acctgactgc cgttatcacc ccggacggca accggagcga
     2761 gacacagtac gatgcgtggg gaaaggcggt cagcaccacg cagggcgggc tgacgcgcag
     2821 tatggagtac gatgctgccg gacgtgtcat cagtctgact aacgagaacg gcagtcacag
     2881 cgacttcagc tacgatgcgc tggaccggct ggtacagcag ggcggctttg acgggcggac
     2941 gcaacgttat cattatgacc tgaccggaaa actcacgcag agtgaggatg aggggcttgt
     3001 caccctctgg cactacgatg cgtcggaccg catcacgcac cggacggtga acggcgaccc
     3061 ggcagagcag tggcagtatg acggccacgg ctggctgaga gaaatcagcc acctgagcga
     3121 aggccaccgt gtcgctgtcc actacggcta cgacgataag ggccgcctga cgggcgaacg
     3181 gcagacggtg gagaacccgg agacggggga actgctgtgg cagcatgaga cgaaacacgc
     3241 ctacaacgaa caggggctgg caaaccgcgt cacgccggac agcctgccgc cggtggagtg
     3301 gctgacctac ggcagcggtt acctggcggg catgaagctg ggcgggacgc cgctggtcga
     3361 gtatacgcgg gacaggctgc accgtgagac ggtgcgcacc ctcgcaccgg acctggacat
     3421 ccgcatcccg tatgccaccg acccggcggg aaaccgcctg ccggacccgg agctacaccc
     3481 ggacagcacg ctcacggcgt ggccggataa ccgcatcgca gaggatgcgc actatgttta
     3541 ccactatgat gaatacggca gactgacgga gaagacggac cgcatcccga cgggtgtgat
     3601 acggacggac gacgagcgga cccaccacta cgacagccag caccgcctgg tgttccacac
     3661 gcggatacag catggcgagc cactggtcga gagccgctac ctctacgacc cgctgggacg
     3721 gcgaatggcg aaacgggtat ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc
     3781 gcgtaaaccg gaggagacat ggtacggctg ggacggcgac aggctgacga cggtacagac
     3841 cgacaccaca cgtatccaga cggtatacga gccgggaagc ttcacgccgc tcatccgggt
     3901 cgagacagag aacggcgagc gggagaaagc gcagcgccgc agcctggcgg agaagctgca
     3961 gcaggaaggg agcgaggacg ggcacggcgt ggtatttccg gctgaactgg tgcggctgct
     4021 ggacagactg gaggaagaaa tccgggcaga ccgcgtgagc agtgaaagcc gggtgtggct
     4081 tgcgcagtgc gggctgacgg tggagcaact ggccagacag gtggagccgg aatacacacc
     4141 agtgcgaaac gttcatcttt accactgcga ccatcggggc cagccgctgg cgctcatcag
     4201 cgaagacggc gatacggtgt ggagcggggc gtatgatgag tggggcaacc agctaaatga
     4261 ggagaacccg catcatgtgt atcagccata ccgtctgccg gggcagcagt atgatgagga
     4321 gtcggggctg tactacaatc gtcaccggta ctatgatccg ttgcagagac ggtacatcac
     4381 ccaggacccc attgggctga aaggtgggtg gaatctgtat cagtatccgc tgaatccggt
     4441 gagcaggata gatccgttgg ggttattctc atgtgagagt tgtagtcatt ttatagatga
     4501 tataaataat aaactggata accttccgtt taacccgaca aaaatgttta cagctagttt
     4561 aaatactgct aattcagcaa ggctgtatgc aagtggcatg acaaaattcg gtataagtgt
     4621 tgctgccgat ggaacgatca ttgctgcccc agtcggtgca gcttctatgg ctttaggggc
     4681 gtggaatata aaatctgcga cagctgcata taacagagca aatttacaat tatcagaatc
     4741 acttcaggaa aatggtagag ataggtgttg gagaaattta ttgggtattt taccttatgg
     4801 tgagcagttt gatgaccctg gtgaaccatc aatattagac gtttatggtg aaaagattga
     4861 gaatatcaaa gattctccaa tgaagttttt tgaagagctt gggacgctgg ggttgtaatg
     4921 tgtattttta atgatatttt tatgtttgtg tttattgtta gtagtatact tgtaaccgtg
     4981 cattttgtgt attctttatt ttttaaaagt ggaaaggtaa aagataaggt aaaaaaatgg
     5041 attaaagtaa tgtgtgattt attttggggg ataggatagt ggtgtgatat ggataaatat
     5101 aaattacagt ggctggcata tttcgacctg acgccggagt agataaaaaa acagacagga
     5161 ttggagtata gcaagcgtct gacaatctct cgggacagta gtatgatgaa gaggcggggt
     5221 tgtactataa ccgtaaccgg tactacgatc cgctaaaggg caagtatatg actcagggaa
     5281 agatcatcag aagttcactt tttgtactaa ataattcgca ttttatgttt aaaaattgag
     5341 atattcctca ttacctgaag ctgttttttt attgcttata cataatcaaa tactccttac
     5401 ataattaagg agaacaaaat ggaaattaaa aaattgatgg aacatttttt gaagcaatat
     5461 ggtgattttg aaaatggcat tcctgttcac gataccattg ccagagttgt atcctgtatc
     5521 agtcctgcaa aatttcatga gtgctttatt aactggatgc gtgactgcca ttcttcagat
     5581 gataaagacg tcattgcaat tgatggaaaa acgctccggc actcttatga taagagtcgt
     5641 cgcaggggag cgattcatgt cattagtgcg ttctcaacaa tgtacagtct ggtcctcggg
     5701 cagat
//
LOCUS       DAEEBB010000202         5640 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEEBB010000202 DAEEBB010000000
VERSION     DAEEBB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.202"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="KLF07_003910"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="KLF07_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBD9833787.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="KLF07_003911"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="KLF07_003911"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBD9833788.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="KLF07_003912"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="KLF07_003912"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBD9833789.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="KLF07_003913"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="KLF07_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBD9833790.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAEEBB010000203         5620 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEEBB010000203 DAEEBB010000000
VERSION     DAEEBB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.203"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..513)
                     /locus_tag="KLF07_003914"
     CDS             complement(<1..513)
                     /locus_tag="KLF07_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBD9833791.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="KLF07_003915"
     CDS             complement(759..1211)
                     /locus_tag="KLF07_003915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD9833792.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="KLF07_003916"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="KLF07_003916"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBD9833793.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="KLF07_003917"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="KLF07_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBD9833794.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="KLF07_003918"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="KLF07_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="KLF07_003919"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="KLF07_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBD9833795.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="KLF07_003920"
     CDS             complement(5494..>5620)
                     /locus_tag="KLF07_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833796.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAEEBB010000204         5614 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEEBB010000204 DAEEBB010000000
VERSION     DAEEBB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.204"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="KLF07_003921"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="KLF07_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBD9833797.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="KLF07_003922"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="KLF07_003922"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBD9833798.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="KLF07_003923"
     CDS             2475..4124
                     /locus_tag="KLF07_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBD9833799.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="KLF07_003924"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="KLF07_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAEEBB010000205         5537 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEEBB010000205 DAEEBB010000000
VERSION     DAEEBB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.205"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..115)
                     /locus_tag="KLF07_003925"
     CDS             complement(<1..115)
                     /locus_tag="KLF07_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBD9833800.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="KLF07_003926"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="KLF07_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBD9833801.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="KLF07_003927"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="KLF07_003927"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBD9833802.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="KLF07_003928"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="KLF07_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBD9833803.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="KLF07_003929"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="KLF07_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBD9833804.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="KLF07_003930"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="KLF07_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBD9833805.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5350..>5537)
                     /locus_tag="KLF07_003931"
     CDS             complement(5350..>5537)
                     /locus_tag="KLF07_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HBD9833806.1"
                     /translation="VDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAI
                     ARAQAKKAAQQKVVNED"
BASE COUNT         1454 a         1406 c         1364 g         1313 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt
     5461 agtctgttgc tgttccagct tgcgcgcttt ggcacgggca atagcggctt cgacggcggc
     5521 tttgcgcgga tcaacct
//
LOCUS       DAEEBB010000206         5527 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEEBB010000206 DAEEBB010000000
VERSION     DAEEBB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.206"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="KLF07_003932"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="KLF07_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBD9833807.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="KLF07_003933"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="KLF07_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBD9833808.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="KLF07_003934"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="KLF07_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBD9833809.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="KLF07_003935"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="KLF07_003935"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBD9833810.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="KLF07_003936"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="KLF07_003936"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBD9833811.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="KLF07_003937"
     CDS             4447..4812
                     /locus_tag="KLF07_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBD9833812.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="KLF07_003938"
     CDS             4962..5480
                     /locus_tag="KLF07_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBD9833813.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1520 a         1391 c         1240 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAEEBB010000207         5477 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEEBB010000207 DAEEBB010000000
VERSION     DAEEBB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.207"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="KLF07_003939"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="KLF07_003939"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBD9833814.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="KLF07_003940"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="KLF07_003940"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBD9833815.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="KLF07_003941"
     CDS             complement(2698..3390)
                     /locus_tag="KLF07_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBD9833816.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /locus_tag="KLF07_003942"
     CDS             3520..5280
                     /locus_tag="KLF07_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBD9833817.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1409 a         1399 c         1282 g         1387 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactg
//
LOCUS       DAEEBB010000208         5431 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEEBB010000208 DAEEBB010000000
VERSION     DAEEBB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.208"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            92..682
                     /locus_tag="KLF07_003943"
     CDS             92..682
                     /locus_tag="KLF07_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HBD9833818.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            819..1391
                     /locus_tag="KLF07_003944"
     CDS             819..1391
                     /locus_tag="KLF07_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9833819.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            1428..2819
                     /locus_tag="KLF07_003945"
     CDS             1428..2819
                     /locus_tag="KLF07_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HBD9833820.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            2864..3280
                     /locus_tag="KLF07_003946"
     CDS             2864..3280
                     /locus_tag="KLF07_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9833821.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(3599..4255)
                     /locus_tag="KLF07_003947"
     CDS             complement(3599..4255)
                     /locus_tag="KLF07_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HBD9833822.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            4650..5431
                     /locus_tag="KLF07_003948"
                     /pseudo
     CDS             4650..5431
                     /locus_tag="KLF07_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1408 a         1303 c         1415 g         1305 t
ORIGIN      
        1 agagcatgaa aaacctcggg gcagccatcg aatgatgcca tctacagggg ttaaaataac
       61 caccatcatt tcataacaat acctgctgac tatgaagatc tacgtcatca aaatagcagt
      121 tcatggagtc agtccgatgg tctggcgtcg gctgagaatt gctgccgaca cgtcactggc
      181 cgcgcttcac ttcattttcc agatagtgca gggctggggg gacgaccatc tccatcaatt
      241 tcacatttat ggcaaagatt acggtatctc ctacgaagga ggcatcggtt ttgtcgataa
      301 tccgttccgg gtcgtgattg atgattttgc ttttgatgct ggcgatcgct ttacctatga
      361 atacaatttc tttgagcact ggcttcatga cattcgtgtt gaggccattt atgaaaactc
      421 tacgctgaaa gcgccgtttt gtataagcgg ccatggtatg cccggagcca cagctgcgga
      481 tgagttcgat aaaaccctgg cgtttcttga agctatcgtt aatgcggatg atgaaacaac
      541 ggtcggcgag atccgccctt ttgctgacga cctggatgct gtcaggttca accgccacaa
      601 aatcaaccgg caactgagca ggctcgacct tgcatctccg gtactggagc ctgaagttat
      661 ctggctgggc cgtcgtcgct gactgatgtc tataaaggta tacgttttca gcatgcctct
      721 cagatccccg aaaaaatgtc tataaacctc cctaaatcag tgtttatgga ttattcttta
      781 aatctctatg atgtttaaag accttccgga ggttttgcgt gcaatatgcc tacatcaggg
      841 taagctcggc cgatcagaat acggcccgtc aggaagaggc attgtcaaag gcgggttttc
      901 agcctgataa aatttgtgtt gagcatgcca gcgcgaagga cacaaaccgc ccggggttac
      961 aggaactcct ggggcaatta cgccctggtg atacgcttct ggttcattcc atcgatcgcc
     1021 tttgccggaa tatgtcggat atgtgcgctg tcaccacccg gcttcgtgac cagggcgtta
     1081 cccttatttt tctgaaagag cagctgacgt tcagcgccgg cacaaacaat ccgatgcagg
     1141 aactgcagct gcacatgatg tcggcattca gccagtttga acgagcctta ctgaaggaaa
     1201 ggcaggccga cggcatcgcc gcgaaaaaag cacggggtga aaaaacaggg cgccccggcg
     1261 ccgatatcaa aaagatccag gaaattgacg cactcagaag cagaggggtc aggctcagga
     1321 tcgcctgcga ccatgcgggc cttggcgttt ccacatatta taagcttcgt caccagatga
     1381 acaaccagta gccgggagat agtcatccac cacagcagga ggcaatgatg caactgacgc
     1441 tccagatagt catcaccgat gaatcaggct ccagtcgaac ggaggaactg atgacaatcc
     1501 agaaatcagg ggagacccgg aacgacatcg gattatcggt gtcagaatcc aagctgttgc
     1561 tgaatacggt ccagcagtcg gtggtccagc tgcaggcaga cgaatatact caacaccata
     1621 tccggtgccc tcactgcctt gctgcgcgca gaatcaaagg caaacagaaa atacggtacc
     1681 ggacgctgtt tggcgttatt ccggtatccg ggcttcgggt gtaccggtgc cgttgtgaag
     1741 agagtgatac aaagacagtc agtttgctca gcgactgggc cggcgattat tctcacccgg
     1801 cactgaaata tattgaaacc cgctgggcct cgatgatctc ctatgaaatg acgactcgtc
     1861 tgctgaaaga tattctgccg gtgggccaca gcctgaatgc ctcaacggtg aggaatcatt
     1921 tatgccaggt ggcgcagcgt cttgacgctg aggctgaagc tcattcaggt tttctttccg
     1981 gctgcccccg cgactggggg aacctgccca ggccaggaaa accgcttgtg gtgggtatag
     2041 atggtggtta tgttcgcgat cgggatgaca aaaagcgtaa ttttgaaatt attgccggga
     2101 agtctttttc cgttggtgcg cctgctgaca cccgccgctt cggttttgtt cagaaggatg
     2161 actgtcatcc ggaacgcagg ctcatgaccc acctttcagc gcagggaatg caggccaacc
     2221 agcagatatt ttttctgtcc gacggcgcgg ataatctcag ggacctccag ttcggtatgt
     2281 accctgagtc aacgcatgtg ctggactggt ttcatatcac catgaggctg aaggtgctca
     2341 tgcaatatgc ccggggactg ctggtatcag atccagaggc ggggagtaaa gttctggcac
     2401 tgctggaaag cattaaacga tatctctggc atggcaatgt tgtcgctgcg ctggaacata
     2461 ttgataattg cgttatgtat tgcgatgacc ctgaactcag ctatcctagc ctgaaatccc
     2521 tgcaaaaaca tctggatgaa atgtatacct acatccggaa taataagatg atgatcccaa
     2581 actacggcga aatgcgtcgg tacggagagc cggtatcaac cgcgtttgtg gaatccacga
     2641 tcaatgaagt gatcgccaga cgaatggcca aaaagcagca gatgcagtgg agcagaaaag
     2701 gggctcatta tttgttacag acccggaccg ccgttttgaa taacgaactg caggataaat
     2761 ttgtctgctg gtatccgggt tttcaaagtg acgggaaggg accagcgatg gcagcatagc
     2821 tcacgctgcc ccaatatttt ttatgctctc ggtcgtatgg aagctggacc gcctcggtcg
     2881 tgatcttcgt catctcatta atacagtgca cgacctgacc gcgaggggga ccggtctgaa
     2941 agtgctgacc ggtcacgggg cgactatcga cacaacaacg gccgctggca agcttgtctt
     3001 cggtatcttt gccgcactgg cggagtttga gcgcgaactg attgctgaaa gaacgactgc
     3061 aggtctggca tcagcaagag cccgtgggcg gaacggcggc cgaccttata aaatgacgcc
     3121 agtcaaactg cgactggcaa tggcatcaat gggacaatca gaaacaaagg tcagtacgct
     3181 atgccaggaa cttgggataa cccggcaaac tttgtacagg catatttccc ctgttggtca
     3241 actgcgggcg gacgggatca agctgctcaa ccgtggataa tttgaacgga cggaaaaatc
     3301 tggggttccg gcgtagtacc cctatcacct ccacattttt gcaacagtgc cttataaatg
     3361 gcagccttat gtttacttgc caagatgggc taccatatga tgttttatac tgagcaatat
     3421 taatattatc tttaatggaa aaattaagcc cactaagtga cccactgcta tatggctcta
     3481 ttgtgaagac gcctgagggt aaaaatttat acatggttgt ccctatgtct attattgcaa
     3541 ggttgacaat attattcgtt ttataaattt gagcgtttaa aatcacacat caaagcaatt
     3601 agtcaggata aacaacaata cccagtgctt cgagaatcag ttcttgctgc caggctgcaa
     3661 ttttgatacc tgatgtatcg actttgcggg gatctaaacc gtcgagttcg gcgtggcata
     3721 aattggcacc ctgtaggcta aattgccccc agacatcttc ggaaaaaaca cctcgactta
     3781 agtctgactc tttcagtgat gcacccgcta ggttcgttcc tatccagcga ttttcaaaca
     3841 actcacattt ttctaaacaa accctctcca tattggcata ggaaagatta catccagaaa
     3901 taaatgctga gcaaaagtac atacgattac tcacttgatg ggcaaagttt gttcgggaaa
     3961 agttggcacc ttttaaatca cacgcacgga actctatacc gtagcaattg gcattactga
     4021 agtttgccat cgcaagttgg cattgttgga aacttgcatc acgaagatct gcgacatcaa
     4081 agtggcagcc ttcgatatca ccctgttcaa tgaacttgca gttgacgaat gtcgtatcac
     4141 gcaagttagc acgtcgaaag tcgctgcgaa tgaatgtgca agcggtgaag gtgagatcac
     4201 ttaagtcttt atgtgaaaag ttgtggtgcc gatatgtatg attgtaggtt tccatgagta
     4261 ctccttgttg aaaggagggc tagactacca ttatacatat gccaagacat taaaataaaa
     4321 gcctgttaca tcaacacatt acaacccctc aatttacctg ttttaagtgg tcaaattggc
     4381 gcgtagagcg cctatcgctc ttgcattggc acgcggccct gttgaaaaaa gttattcgct
     4441 ttagataggg ttaggagagc cgtgcttcgc cggtaaaaaa tcggactatc cgacgcaggg
     4501 cggactcatc ggcggagaag caaactttga ctatatctcc ctacacttgg aagagggcta
     4561 gactggcccc atagatcacc agacacttcg actctcttaa aataagaggt agtcttggaa
     4621 ctatgtttaa taccagtcag aacgataaga tggttgaagt gttatcagga cctgagcggc
     4681 gtcggcgtcg tacaccgcag gaaaaaattg ccattattca gcagactatg gagcccggta
     4741 tgaccgtgtc tcatgtcgcg cgcctgcatg gcatcaatgc aaaccagata ttcaaatggc
     4801 gcaagcaata ttaagacggt tcactgacgg ctgttgcatc gggcgaggaa gtggtgccgg
     4861 cctccgaact cgctgccgcc aataagcaaa ttcgcgaact ccagcgcctt ctgggcaaaa
     4921 agacgatgga agccgagata ctcaaagagg ccgtggagtt cggccgggca aaaaaatgga
     4981 ttgcgcatgc gccctcgttg cctgggaacg acgaataagc gacgtttgca gaagtcttgg
     5041 cgtgtcgcgt gcgcagttga gcattcgggt tcaccggcca tctgactgac aggatcgcag
     5101 acggcagccc cgttctgacg ataccaccgt attgtcccgg ataaacatgg cggtggctga
     5161 cctgccgacg tatggttatc gccgtgtgtg ggcgctgctg cgacgggagt cggagcggga
     5221 cggcctgcct gtggttaatg caaagcgggt gtatcgcatt atgagaacgc ataatctgct
     5281 tcttgaacgt aaacccgctg atccttgccg taagcgggcc cataaggggc gcgtagcagt
     5341 agcggaaagt aaccggcgct ggtgctcaga tggctttgag ttccgctgtg ataatggcga
     5401 aaagctgcgg gtgaccttcg cgcaggactg c
//
LOCUS       DAEEBB010000209         5360 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEEBB010000209 DAEEBB010000000
VERSION     DAEEBB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.209"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..452)
                     /locus_tag="KLF07_003949"
     CDS             complement(<1..452)
                     /locus_tag="KLF07_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBD9833823.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVT"
     gene            complement(507..1254)
                     /gene="traX"
                     /locus_tag="KLF07_003950"
                     /pseudo
     CDS             complement(507..1254)
                     /gene="traX"
                     /locus_tag="KLF07_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            complement(1274..2272)
                     /locus_tag="KLF07_003951"
                     /pseudo
     CDS             complement(1274..2272)
                     /locus_tag="KLF07_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2267..2578)
                     /locus_tag="KLF07_003952"
                     /pseudo
     CDS             complement(2267..2578)
                     /locus_tag="KLF07_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2575..2961)
                     /gene="trbI"
                     /locus_tag="KLF07_003953"
     CDS             complement(2575..2961)
                     /gene="trbI"
                     /locus_tag="KLF07_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBD9833824.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(2958..4208)
                     /locus_tag="KLF07_003954"
                     /pseudo
     CDS             complement(2958..4208)
                     /locus_tag="KLF07_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(4210..4392)
                     /locus_tag="KLF07_003955"
                     /pseudo
     CDS             complement(4210..4392)
                     /locus_tag="KLF07_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4414..4725)
                     /gene="traL"
                     /locus_tag="KLF07_003956"
     CDS             complement(4414..4725)
                     /gene="traL"
                     /locus_tag="KLF07_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBD9833825.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(4740..5090)
                     /gene="traA"
                     /locus_tag="KLF07_003957"
                     /pseudo
     CDS             complement(4740..5090)
                     /gene="traA"
                     /locus_tag="KLF07_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(5136..>5360)
                     /locus_tag="KLF07_003958"
     CDS             complement(5136..>5360)
                     /locus_tag="KLF07_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833826.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT         1143 a         1647 c         1338 g         1232 t
ORIGIN      
        1 gtcacatacc cttccgtgtc ataccggcag gcaccggctt tcatggcaca cagatagctt
       61 tctgaacggg tgatggcctt cagcgccctg cgcagttttt tatgcgagag cgggatattc
      121 cgctgcgcca cgtcttccag taacacgtcc cggataccgc aggccagaag ccggggggtg
      181 tcaccgtcaa ataatcccgg ccaccagggt ttcagggtat tcacggcctc atccagcgtg
      241 ggcaggttca gataaatgga cagtgcctgt ctggcctgcg ctttttttgc cgccagctct
      301 gcttcccggg ctgctttctc ggccagcttc tgctttttca ccttccactt tggtggcgtg
      361 gtgacattga tgatggtttt ccggctgcgg acaggcgtct ccccttccgt tttccgcttc
      421 agtgtcagta ccggtcgctt ctgctctgtc atgctgtgtt ctgcctctga cgaatcatcc
      481 tgttacggtc aaagatacca cacccgtcac agcgccagaa cgcccagcac agccagatga
      541 caggcgtaga ataccgggaa aaaatccccg ggccagaaac ggggtaatga ttttcctgca
      601 cacgacacca gaccaacggt cagcaccgtc atcacaagac cggccaccgc cgctgcatca
      661 ctggtggcaa ggttaagcgc cggaataaca gccagcaggc aggcgaccag cgccagacgt
      721 tccgctctgt cttccgcccg gtacagtcgg tggctgactg ccagcatcag cagcccggca
      781 atgccgtaac tggtgccgga caaaggcccc caacagggcc atcagaagaa tggccgctgc
      841 tgtacgccac ccactgcgcg tttcacacca cgtcagcacc tgtgccgcca ctgcaaaggc
      901 aaacaggata ttcccctcat accagggaaa gcctgccaga taatatgcga actgggcaat
      961 aattccccat ccccacagcc ggttaatggc cggttgccgg atatgcgcat gacgggacag
     1021 attcagcccc cacaccaggg caaaaagcgg aaaggctcct cgcccggcca gaaacatcca
     1081 ttcctgcttc agctggaata tcagattgat gtggtccagt accatcagca ccagtgccac
     1141 cgttttgatg atatcccgct gtccgggtga ccagaccaga aatgactgca accaggtatc
     1201 ggtccgtgca gccagtgaat cgttacgtgt cgtgttcgtg ttatccgttg tcatcagtgt
     1261 ttgtcttcct gtatcagtct ccgcccgggg ttttctcttt ctgcaggtca cgaaccatat
     1321 cccgctccat ctgctgcaga cgctcctgca gcagattttc ccgggcaacc tcccggaccg
     1381 cttcccgctc ccgttgtgat tcacgcagca cactctccgg cagcgcggct tcccgttcag
     1441 ttatggcagc ccggtcacgc tcctgcccgg caatttccct gacagccagc tctgttttgc
     1501 cgtccggcag gtcaggtttg ttctctgcca ttttacggac aatttcatct gcgcgacgtt
     1561 ccgtttcacg gcggatggcc tcttcagcct gccgctgtgc cagtacctct gccgtgaccg
     1621 gttcgccatt tccggctccc ggctggacgc tgttgtccgg gatatccccc cacacgcggc
     1681 catcggtgat ggcaccggga ttccacggac ggccttcacc ggcaatgctt accaccacac
     1741 cactgtcagg attatcacgg gcaatccgga cgccatcctg catattatca gccagcaggc
     1801 tttctccgtt acggctgccc tgcagggcca cgaactgcgc atccccgctg ccttttaccc
     1861 gaccttcacc gctgaatccc cgcagcccgt ttccgtcatc cgtggtcagc gggttcagcc
     1921 agataccggc ggacttgccg ttacggtcaa acgccggcag tgccacatac ggttgcggat
     1981 atttacgtcc cggagcaata aaccgtgcag gactgtctcc cccggccagc cccgcctgac
     2041 ggagaacggc acggcctgcc gccacgtccc gcagctcccg cgccgtactg aacagccgct
     2101 ctgcattcat gacctcccgg tccggtttcg gctcaaacac atcgtgggca gttcctttct
     2161 gtacggcatt gttgatggca tccgtccagc cctgacggtt atcggtgtac acctgcacat
     2221 gctgcttcat acgcgacagg gccacgtagg ctgactcaaa gccggccacc cgcccttcgt
     2281 tatcccggat atccgcagcc agtctgacgg acgggtcaaa caaccggctg ctgtatttct
     2341 ccgtgcgtcc gataccgggc acggcagggg gccgcagact gttgcggatc acccgctcct
     2401 taaaggcatc cattttccgg cccatctcac cggactgctc cagtgccgtc aggcgctgca
     2461 tgatcaccgt cagcatgtcc ggctccttta ccggccacag atcaccccag gtgccaagat
     2521 cggcggcggc cacactctgc ccccatatca gcagggcaat cagcccccgg catctcatgg
     2581 ttccgctctc attcgccggg caatatcctg ctggatttcc cgggtgatat ccggagcccc
     2641 ctgtaccacg gcaggcgaca ccagaatgac cgcgtggtgt ttctgctgcc acgcctgcag
     2701 gctggcttcc agtgccgtgt taaaacgcgc tgaaagggct tttgactgtg cttctgacag
     2761 ttgtttctga ctggcgctgt caaaaaatgc atccacagtc tgtttcatgt tgaaggcggc
     2821 agtcatcggt gcgttcagtc tgacaatgcc gtaactgacg gctgcgttca gtaacaccat
     2881 tgccaggcat cccggcaccg tccaccacca gtggctgcgt cggggggctg taacatcagt
     2941 cgttttttgc gttgtggtca tgcaacactc ctgtatttct catgttcttc cagccaggct
     3001 tccagcgaag ccatctccgg ccctgacttc ttccacgcca gctgccacac cgcctcatga
     3061 atactcagcc cctctttacg tttctgctgc acaaactcaa aatccgggcc gtcagagctg
     3121 tacatggcgc ggcttaacgg gtccacaaac aggcggtgcc aggaggaatg gttttccacc
     3181 tgcagcagga aggaactgaa ccactggtct ttggccgcac caaacttacc aatcatgtcg
     3241 cgctgcagtg gctggaactg gtccggatac agctggttgt attttgcgaa ctccttcgca
     3301 ctctgtttga ggataatttt gtaggaggag ttaccccatg ccgcacgggc cgcactggag
     3361 gccttgtcag agtcaaagtc gacgatgttc tgtgtgatgg tgatataagc accggtatga
     3421 cgacgggcgg tacggtaacc tttctcaata aactcaccga ccttgtgatt tttgaagtcc
     3481 agcagacgcc agccttcatc aatgacgttc agtttcttga ggttacgcgg cgtgcggtac
     3541 atcctgttct cgatgtagat aatcagggag aacatcaccg caaccagcag tgacggacgg
     3601 tcttccagtc cgcccagctc cagcaccacc atttttgcgt catcccgcag ggacggctca
     3661 tcagagttaa aatactggcc gtaagtgccg ttggcagtgt actggtcaag cagcacaatc
     3721 atttcgtcca gacggctgcg gatagtcggt gacccggcat actgctcgct gtcactggcg
     3781 tttttcagga aatccaccac gtcatcaata cgtgccctgt tctctttggc cagccaggag
     3841 gccctgaccg cctgcagcag cagaccttca tgcacttcat ccaggttacc gttggggctg
     3901 gccatcactg acaactggtc acggacacgc tccgctgact ggtcgatatc ggtgatgttc
     3961 gcaaacgggt taaaacgcag ggtttcaccg tccagatata cgccacccat gttctcacac
     4021 agggacttgt agccatcccc catgtcgaac accacggcaa agcccccgga gtccagcacg
     4081 ctgcggataa gtggctgtat cagcccggtt ttaccggccc cggaggtgcc acagaccgcc
     4141 atgttgtagt tggtgttatt cattccccgg aagaaaatat cgataaacgc cagctggtta
     4201 cggtaggtgt gacacggcaa agggcacatt aaatcccatt ggggtcacgg cagtgcgttg
     4261 ctcattctga agacggtagt tattcacccc ctgaatgagg ttaacagaga ggctgagaac
     4321 aataagcact gacataaata taaaggcgat ggccattaca cgactggtac ttaaacgggc
     4381 accgtgttcc atataacaat cctggtatca gttctattta atccactgcc ggaaacacga
     4441 atcgggaaca ttatgaaaaa taccgcgcag cagggctgtt ggcatatacc agtaaatcag
     4501 gtcacgtaac caggaactgc cccgcccttt tttcagtttt ttaatcccga aataaaccag
     4561 aaccgctgca ccaataccga acagatattt cgatgttgtg ataccccagc caacacagat
     4621 tgctgcgggg atcagttcat ccagtggcag gccaaaccag cggctctggt tggtcagtgt
     4681 ttccgggaaa cgatatttct taagtttatt ctcgtctccc aacatcgttt tatttcctgt
     4741 cagaatccgg caacagacat acctactgca ataaatacag agagtatggc aaagccgacc
     4801 aggaacttga cgtttttggt catcatgtac atgactgcac cgaccagaac ttcagccaga
     4861 acaacccatt taacgatact ggagtcctta ccgaaggtcg ccttaaccgt ttcctgacct
     4921 ttagccatca ggtccgtgcc tttaccagca gccatagcta actcagggaa caacatcatc
     4981 agaacagcaa ccgggatcac tgcacgaacc aggcgaagca tattcagacg agtgaattta
     5041 gaaaaaaacg atttcttttt gacgggcgca gaagtaccct gaacacttaa cgctgaaccg
     5101 ccccgggttt cctggagagt gttttatctg tgaactcagg ctgccagatc atcgtttccg
     5161 atggaagcat aataagcttt ttctgcttct gccggaggag tatggcccag cctttccagc
     5221 aatcgtcgat tgttatacca gtccacccac gtgagtgtgg ccagctccac ttctgtccgg
     5281 tttttccagc tcttacggtg tattacctcc gctttgtaaa ggcccttgat gatctctgcc
     5341 atcgcgttgt catacgagtc
//
LOCUS       DAEEBB010000210         5332 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEEBB010000210 DAEEBB010000000
VERSION     DAEEBB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.210"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            177..1034
                     /gene="focA"
                     /locus_tag="KLF07_003959"
     CDS             177..1034
                     /gene="focA"
                     /locus_tag="KLF07_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBD9833827.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1089..3371
                     /gene="pflB"
                     /locus_tag="KLF07_003960"
     CDS             1089..3371
                     /gene="pflB"
                     /locus_tag="KLF07_003960"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD9833828.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3563..4303
                     /gene="pflA"
                     /locus_tag="KLF07_003961"
     CDS             3563..4303
                     /gene="pflA"
                     /locus_tag="KLF07_003961"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBD9833829.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4396..4572)
                     /locus_tag="KLF07_003962"
     CDS             complement(4396..4572)
                     /locus_tag="KLF07_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBD9833830.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            4672..>5332
                     /locus_tag="KLF07_003963"
     CDS             4672..>5332
                     /locus_tag="KLF07_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9833831.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1371 a         1308 c         1278 g         1375 t
ORIGIN      
        1 atttgttaat ttttaaaatt tatttttatt tggataatca aatgtttact ccgtatctgc
       61 ataaaaacca tgcgagttac gggcctataa gccaggcgag atatgatcta tatcaatttc
      121 tcatctataa tgctttgtta gtatctcgtc gccgacttaa taaagagaga gttagtgtga
      181 aagctgacaa cccttttgat cttttacttc ctgctgcaat ggccaaagtg gccgaagagg
      241 cgggtgtcta taaagcaacg aaacatccgc ttaagacttt ctatctggcg attaccgccg
      301 gtgttttcat ctcaatcgca ttcgtcttct atatcacagc aaccactggc acaggcacaa
      361 tgcccttcgg catggcaaaa ctggttggcg gcatttgctt ctctctgggg ctgattcttt
      421 gtgttgtctg cggagccgat ctctttactt ccaccgtgtt gattgttgtt gctaaggcga
      481 gtgggcgcat cacctggggt cagttggcga aaaactggct aaatgtctat tttggcaacc
      541 tggtcggcgc actgctgttt gtacttttaa tgtggctttc cggcgagtat atgaccgcaa
      601 atggtcaatg gggactaaac gtcctacaaa ccgccgacca caaagtgcac catactttta
      661 ttgaggccgt ctgtcttggt atcctggcaa acctgatggt atgtctggca gtatggatga
      721 gttattctgg ccgcagcctg atggacaaag cgttcattat ggtgctgccg gtcgcgatgt
      781 ttgttgccag cggttttgag cacagtatcg caaacatgtt tatgatcccg atgggtattg
      841 taatccgcga cttcgcatcc ccggaatttt ggaccgcagt cggttctgca ccggaaaatt
      901 tttctcacct gaccgtgatg aatttcatca ctgataacct gattccggtt acgatcggca
      961 acattatcgg tggtggtttg ttggttgggt tgacatactg ggtcatttac ctgcgtgaaa
     1021 acgaccacca ttaatggttg tcgaagtacg cagtaaataa aaaatccact taagaaggta
     1081 ggtgttacat gtccgagctt aatgaaaagt tagccacagc ctgggaaggt tttaccaaag
     1141 gtgactggca gaatgaagta aacgtccgtg acttcattca gaaaaactac actccgtacg
     1201 agggtgacga gtccttcctg gctggcgcta ctgaagcgac caccaccctg tgggacaaag
     1261 taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc agttgacttt gacaccgctg
     1321 ttgcttccac catcacctct cacgacgctg gctacatcaa caagcagctt gagaaaatcg
     1381 ttggtctgca gactgaagct ccgctgaaac gtgctcttat cccgttcggt ggtatcaaaa
     1441 tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga tccgatgatc aaaaaaatct
     1501 tcactgaata ccgtaaaact cacaaccagg gcgtgttcga cgtttacact ccggacatcc
     1561 tgcgttgccg taaatctggt gttctgaccg gtctgccaga tgcatatggc cgtggccgta
     1621 tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga ctacctgatg aaagacaaac
     1681 tggcacagtt cacttctctg caggctgatc tggaaaacgg cgtaaacttg gaacagacta
     1741 tccgtctgcg cgaagaaatc gctgaacagc accgcgctct gggtcagatg aaagaaatgg
     1801 ctgcgaaata cggctacgac atctctggtc cggctaccaa cgctcaggaa gctatccagt
     1861 ggacttactt cggctacctg gctgctgtta agtctcagaa cggtgctgca atgtccttcg
     1921 gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga cctgaaagct ggcaagatca
     1981 ccgaacaaga agcgcaggaa atggttgacc acctggtcat gaaactgcgt atggttcgct
     2041 tcctgcgtac tccggaatac gatgaactgt tctctggcga cccgatctgg gcaaccgaat
     2101 ctatcggtgg tatgggcctc gacggtcgta ccctggttac caaaaacagc ttccgtttcc
     2161 tgaacaccct gtacaccatg ggtccgtctc cggaaccgaa catgaccatt ctgtggtctg
     2221 aaaaactgcc gctgaacttc aagaaattcg ccgctaaagt gtccatcgac acctcttctc
     2281 tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa caacgatgac tacgctattg
     2341 cttgctgcgt aagcccgatg atcgttggta aacaaatgca gttcttcggt gcgcgtgcaa
     2401 acctggcgaa aaccatgctg tacgcaatca acggcggcgt tgacgaaaaa ctgaaaatgc
     2461 aggttggtcc gaagtctgaa ccgatcaaag gcgatgtcct gaactatgat gaagtgatgg
     2521 agcgcatgga tcacttcatg gactggctgg ctaaacagta catcactgca ctaaacatca
     2581 tccactacat gcacgacaag tacagctacg aagcctctct gatggcgctg cacgaccgtg
     2641 acgttatccg caccatggcg tgtggtatcg ctggtctgtc cgttgctgct gactccctgt
     2701 ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga agacggtctg gctatcgact
     2761 tcgaaatcga aggcgaatac ccgcagtttg gtaacaacga tccgcgtgta gatgacctgg
     2821 ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa actgcacacc taccgtgacg
     2881 ctatcccgac tcagtctgtt ctgaccatca cttctaacgt tgtgtatggt aagaaaacgg
     2941 gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg accgggtgct aacccgatgc
     3001 acggtcgtga ccagaaaggt gcagtagcct ctctgacttc cgttgctaaa ctgccgtttg
     3061 cttacgctaa agatggtatc tcctacacct tctctatcgt tccgaacgca ctgggtaaag
     3121 acgacgaagt tcgtaagacc aacctggctg gtctgatgga tggttacttc caccacgaag
     3181 catccatcga aggtggtcag cacctgaacg ttaacgtgat gaaccgtgaa atgctgctcg
     3241 acgcgatgga aaacccggaa aaatatccgc agctgaccat ccgtgtatct ggctacgcag
     3301 tacgtttcaa ctcgctgact aaagaacagc agcaggacgt tattactcgt accttcactc
     3361 aatctatgta attagatttg actgaaatcg tacagtaaaa agcgtacaat aaaggctcca
     3421 cgaaagtggg gcctttttta gcacgagagc cttttttgtc agctatctat actctaaggt
     3481 gactgccaaa acagactcga cgtagccttc gagctgcgca ccaacacggc ctcagatggg
     3541 ccacatctgg agaaacaccg caatgtcagt tattggtcgc attcactcct ttgaatcctg
     3601 tggaaccgta gacggcccgg gtattcgctt tatcaccttt ttccagggct gcctgatgcg
     3661 ctgcctgtat tgtcataacc gcgacacctg ggatacgcat ggcggtaaag aagttaccgt
     3721 tgaagattta atgaaagagg tggtgaccta tcgccacttt atgaacgctt ccggcggcgg
     3781 cgttaccgca tccggcggtg aggcaatcct acaagctgag tttgttcgtg actggttccg
     3841 cgcctgcaaa aaagaaggca ttcatacctg tctggacacc aacggttttg ttcgtcgtta
     3901 cgatccggtg attgatgaac tgctggaagt aaccgacctg gtaatgctcg atctcaaaca
     3961 gatgaacgac gagatccacc aaaatctggt tggagtttcc aatcaccgca cgctggagtt
     4021 cgccaaatat ctggcgaaca aaaatgtgaa ggtgtggatc cgctacgttg ttgtcccagg
     4081 ctggtctgac gatgacgatt cagcgcatcg cctcggtgaa tttacccgtg atatgggcaa
     4141 cgttgagaaa atcgagcttc tcccctacca cgagctgggc aaacacaaat gggtggcaat
     4201 gggtgaagag tacaaactcg acggtgttaa accaccgaag aaagagacca tggaacgcgt
     4261 gaaaggcatt cttgagcagt acggtcataa ggtaatgttc taatttcccc gtaaagcggc
     4321 aactttattg agttgccgct tttttattcc gcacttcatt ctgctacagc gtttttaaca
     4381 catcttatag tgcatctacc atatcccgta cctgagataa taacgactga taatattacg
     4441 ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct gatggatcgc
     4501 atcagcaata tgtgcggtca atgaatcatg acgaggatgg gcccatacca aataaatacg
     4561 ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac gcaggcggca
     4621 taataatgta aacgtgatga ttcaggatga gttaatgaag gattttcact aatgcggatg
     4681 aatatgtctg actttgccac tttctttgct gtggcccgta atcaaagctt tcgtgcagcg
     4741 ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac actggaacaa
     4801 cgtcttaaaa ttcgcttatt caatcggaca acccgaagcg tatcgttaac ggaagcgggt
     4861 tctaatctct atgaacgatt gcgcccagct ttcgacgaaa ttcagatcat gttagatgag
     4921 atgaatgatt ttcgtctgac accaacagga acgctcaaaa tcaatgccgc ccgggtagct
     4981 gcacgtatat ttttaatgcc tttactggtt ggtttcacac gtgaatatcc tgatattaaa
     5041 gtcgagctca caactgacga ctctcttgtc gatatcgtcc aacagggctt tgacgctggc
     5101 gttcgactta gtggcatcgt cgaaaaagat atgatcagcg tggcgatcgg cccaccagtg
     5161 aaattatgtg tagcagcaac gccagagtac tttgcaagat atggcaaacc ccgccatcct
     5221 catgatctgc ttaatcatca atgtgttgta tttcgttatc ccagcggcaa acctttccac
     5281 tggcaatttg caaaggagca ggaaattgct gtcgcaggga atatcattct gg
//
LOCUS       DAEEBB010000211         5315 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEEBB010000211 DAEEBB010000000
VERSION     DAEEBB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.211"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..475
                     /gene="tolA"
                     /locus_tag="KLF07_003964"
     CDS             <1..475
                     /gene="tolA"
                     /locus_tag="KLF07_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBD9833832.1"
                     /translation="AAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAP
                     KTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLR
                     IKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            608..1900
                     /gene="tolB"
                     /locus_tag="KLF07_003965"
     CDS             608..1900
                     /gene="tolB"
                     /locus_tag="KLF07_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBD9833833.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            1935..2456
                     /gene="pal"
                     /locus_tag="KLF07_003966"
     CDS             1935..2456
                     /gene="pal"
                     /locus_tag="KLF07_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBD9833834.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            2466..3257
                     /gene="cpoB"
                     /locus_tag="KLF07_003967"
     CDS             2466..3257
                     /gene="cpoB"
                     /locus_tag="KLF07_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBD9833835.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            3422..3497
                     /locus_tag="KLF07_003968"
     tRNA            3422..3497
                     /locus_tag="KLF07_003968"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3455..3457,aa:Lys,seq:ttt)
     gene            3647..3722
                     /locus_tag="KLF07_003969"
     tRNA            3647..3722
                     /locus_tag="KLF07_003969"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3680..3682,aa:Val,seq:tac)
     gene            3726..3801
                     /locus_tag="KLF07_003970"
     tRNA            3726..3801
                     /locus_tag="KLF07_003970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3759..3761,aa:Lys,seq:ttt)
     gene            3850..3925
                     /locus_tag="KLF07_003971"
     tRNA            3850..3925
                     /locus_tag="KLF07_003971"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3883..3885,aa:Lys,seq:ttt)
     gene            3959..4034
                     /locus_tag="KLF07_003972"
     tRNA            3959..4034
                     /locus_tag="KLF07_003972"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3992..3994,aa:Lys,seq:ttt)
     gene            4059..4190
                     /locus_tag="KLF07_003973"
     ncRNA           4059..4190
                     /ncRNA_class="other"
                     /locus_tag="KLF07_003973"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            4439..>5315
                     /gene="nadA"
                     /locus_tag="KLF07_003974"
     CDS             4439..>5315
                     /gene="nadA"
                     /locus_tag="KLF07_003974"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBD9833836.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         1392 a         1291 c         1384 g         1248 t
ORIGIN      
        1 agcggcagaa aaagccgccg cagacaaaaa agcggcagcg gcaaaagcag cagctgaaaa
       61 agccgctgca gcaaaagctg ccgcagaggc agatgatatt ttcggtgagc taagctctgg
      121 taagaatgca ccgaaaacgg ggggaggggc gaaagggaac aatgcttcgt ctgccgggag
      181 tggtaatact aaaaacaatg gcgcatcagg ggccgatatc aataactatg ccgggcagat
      241 taaatctgct atcgaaagta agttctatga cgcatcgtcc tatgcaggca aaacctgtac
      301 gctgcgcata aaactggcac ccgatggtat gttactggat atcaaacctg aaggtggcga
      361 tcccgcactt tgtcaggctg cgttggcagc agctaaactt gcgaagatcc cgaaaccacc
      421 aagccaggca gtatatgaag tgttcaaaaa cgcgccattg gacttcaaac cgtaatcgcg
      481 atgttgactg ttccgacggt caacatcagg caccggttgc cacggggttc tggtagtttt
      541 gtgtatttta gtttgttaac attctgctaa attatcgtgg gccatcggtc cagataaggg
      601 agatatgatg aagcaggcat tacgagtagc atttggtttt ctcatactgt gggcatcagt
      661 tctgcatgct gaagtccgca ttgtgatcga cagcggtgta gattccggtc gtcctattgg
      721 tgttgttcct ttccagtggg cgggccctgg tgcggcacct gaagatattg gcggcatcgt
      781 tgctgccgac ttgcgtaaca gcggtaaatt taatccgtta gatcgcgctc gtctgccaca
      841 gcagccgggt agtgcgcagg aagtacaacc agctgcatgg tccgcgctgg gtattgacgc
      901 tgtagttgtc ggtcaggtca ctccgaatcc ggatggttct tacaatgttg cttatcaact
      961 tgttgacact ggcggcgcac cgggtactgt acttgctcag aactcgtaca aagtgaacaa
     1021 gcagtggctg cgctatgctg gtcataccgc cagtgatgaa gtgtttgaaa aactgaccgg
     1081 cattaaaggt gcgttccgta cccgtattgc ctacgttgtt cagaccaacg gcggtcagtt
     1141 cccgtatgaa ctgcgcgtat ctgactatga cggttacaac cagtttgtcg ttcaccgttc
     1201 accacagccg ctgatgtctc cggcgtggtc accagacggt tctaaactgg cttatgtgac
     1261 cttcgaaagc ggtcgttccg cgctagttat ccaaacgctg gcaaacggcg ctgtacgtca
     1321 ggtggcttca ttcccgcgtc acaacggtgc gcctgcattc tcgccagacg gcagcaaact
     1381 ggcattcgcc ttgtcgaaaa ccggtagcct gaacctgtac gtaatggatt tggcttctgg
     1441 tcagatccgc caggtgactg atggtcgcag taacaatacc gaaccgacct ggttcccgga
     1501 cagccagaac ctggcattta cttctgacca ggccggtcgt ccacaggttt ataaagtgaa
     1561 tatcaacggc ggtgcgccac aacgtattac ctgggaaggt tcgcagaacc aggatgcgga
     1621 tgtcagcagc gacggtaaat ttatggtaat ggtcagctcc aacggtgggc agcagcacat
     1681 tgccaaacaa gatctggcaa cgggaggcgt acaagttctg tcgtccacgt tcctggatga
     1741 aacgccaagt ctggcaccta acggcactat ggtaatctac agctcttctc aggggatggg
     1801 atccgtgctg aatttggttt ctacagatgg gcgtttcaaa gcgcgtcttc cggcaactga
     1861 tggacaggtc aaattccctg cctggtcgcc gtatctgtga taataattaa ttgaatagta
     1921 aaggaatcat tgaaatgcaa ctgaacaaag tgctgaaagg gctgatgatt gctctgcctg
     1981 ttatggcaat tgcggcatgt tcttccaaca agaacgccag caatgacggc agcgaaggca
     2041 tgctgggtgc cggcactggt atggatgcga acggcggcaa cggcaacatg tcttccgaag
     2101 agcaggctcg tctgcaaatg caacagctgc agcagaacaa catcgtttac ttcgatctgg
     2161 acaagtacga tatccgttct gacttcgctc aaatgctgga tgcacatgca aacttcctgc
     2221 gtagcaaccc gtcttacaaa gtcaccgtag aaggtcacgc ggacgaacgt ggtactccgg
     2281 aatacaacat ctccctgggt gaacgtcgtg cgaacgccgt taagatgtac ctgcagggta
     2341 aaggcgtttc tgcagaccag atctccatcg tttcttacgg taaagaaaaa cctgcagtac
     2401 tgggtcatga cgaagcggca tactccaaaa accgtcgtgc ggtactggtt tactaagaga
     2461 attgcatgag cagtaacttc agacatcaac tattgagtct gtcgttactg gttggtatag
     2521 cggccccctg ggccgctttt gctcaggcac caatcagtag tgtcggctca ggctcggtcg
     2581 aagaccgcgt cactcaactt gagcgtattt ctaatgctca cagccagctt ttaacccaac
     2641 tccagcaaca actttctgat aatcaatccg atattgattc cctgcgtggt cagattcagg
     2701 aaaatcagta tcaactgaat caggtcgtgg agcggcaaaa gcagatcctg ttgcagatcg
     2761 acagcctcag cagcggtggt gcagcggcgc aatcaaccag cagcgatcaa agcggtgcgg
     2821 cggcatcaac gacgccgaca gctgatgctg gtactgcgaa tgctggcgcg ccggtgaaaa
     2881 gcggcgatgc aaacacggat tacaatgcag ctattgcgct ggtgcaggat aaatcccgcc
     2941 aggatgacgc aatggtggca tttcagaatt tcatcaaaaa ttaccctgat tcaacttacc
     3001 tgccaaacgc caattattgg ctgggtcagt taaactacaa caagggtaaa aaagatgatg
     3061 cggcgtacta ttttgcttcg gtagtgaaaa actatccgaa gtcaccaaag gctgctgatg
     3121 cgatgtttaa agtcggcgtc atcatgcagg acaaaggtga caccgcaaaa gcgaaagccg
     3181 tataccagca ggttatcagt aaataccctg gtactgatgg cgctaaacag gcacaaaaac
     3241 gtctgaacgc gatgtaatgc ataacacacg accagaagtc gcattatttc tggtcgtgtc
     3301 gtgcgaatca taagcagttg agtgatctgc atcgaaattt ttgttgcgct caagtctgaa
     3361 atcagtaata tatgccgccg ttgccacggg atatcaaaca aaccgaaagc aacgaaaaag
     3421 tgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa
     3481 tcctgcacga cccaccagtt ttagcatcga agacagatgt taagcgtgta ggataacgtt
     3541 gcgtcagcaa cggcccgtag ggcgagcgaa gcgagtcatc ctgcacgacc caccactaat
     3601 tacggtgggt tcggtggaag tagtttgtag tatccagcgc agtatcgggt gattagctca
     3661 gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac
     3721 cactcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
     3781 cgaatcctgc acgacccacc aatcttaaag attggccccg agtagaaatc tttcaggtaa
     3841 cacccgtatg ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca
     3901 ggttcgaatc ctgcacgacc caccatcctg aatgattaaa gcagcataat cccgcaaggg
     3961 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
     4021 tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttgctat ctgagatatt
     4081 caaagattcg aacctgcagc aggttcgagt tgagcgcagc gaaacaacgg agccgctcgc
     4141 ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca cgacccacca atgtaaaaaa
     4201 gcgccctaaa ggcgcttttt tgctattcag gcatcctcaa tttcactttg taaacctgat
     4261 gacatcgtca gagcttactg tgcaagcaac tctacgtcgg tggaattagg cgtaattcga
     4321 gtgacttttc cccaccattc gactatcttg tttagcatat aaaacaaatt acgccgatag
     4381 tagcgaatat tacgctaatg tcggttttaa cgttaagcct gtaaaacgag atggtaagat
     4441 gagcgtgatg tttgatccag acacggcgat ttatcctttc cccccgaagc cgacgccgtt
     4501 aagcattgat gaaaaagcgt attaccgcga gaagataaaa cgtctgctaa aagaacgtaa
     4561 tgcggtgatg gttgcccact actataccga ccccgaaatt caacaactgg cagaagaaac
     4621 cggtggctgt atttctgatt ctctggaaat ggcgcgcttc ggtgcaaagc atcccgcttc
     4681 tacgttgtta gtcgctgggg tgagatttat gggagaaacc gccaaaattc tcagtccgga
     4741 aaaaacaatt ctgatgctga cacttcaggc tgaatgttca ctggatctcg gctgccctgt
     4801 tgaagaattt aacgcatttt gcgatgccca tcccgatcat actgtcgtcg tctacgccaa
     4861 cacttctgct gcggtaaaag cgcgcgcaga ttgggtggta acttcaagca ttgccgtcga
     4921 acttattgat catctggata gttttggtga aaaaatcatc tgggcacccg acaaacatct
     4981 ggggcgttac gtgcaaaaac agacgggcgc agacatttta tgctggcagg gtgcctgtat
     5041 tgtgcatgat gaatttaaga ctcaggcgtt aacccgcttg caagaagaat acccggatgc
     5101 tgccatactg gtgcatccag aatcaccaca agctattgtc gatatggcgg atgcggtcgg
     5161 ttccaccagt caactgatcg ctgctgcgaa aacattgcca catcagcggc ttatcgtggc
     5221 aaccgatcgg ggcattttct acaaaatgca gcaggcggtg ccagataaag agttactgga
     5281 agcaccaacc gcaggtgagg gtgcaacctg ccgca
//
LOCUS       DAEEBB010000212         5296 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEEBB010000212 DAEEBB010000000
VERSION     DAEEBB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.212"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="KLF07_003975"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="KLF07_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="KLF07_003976"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="KLF07_003976"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBD9833837.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="KLF07_003977"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="KLF07_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBD9833838.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="KLF07_003978"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="KLF07_003978"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBD9833839.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="KLF07_003979"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="KLF07_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAEEBB010000213         5281 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEEBB010000213 DAEEBB010000000
VERSION     DAEEBB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.213"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(38..400)
                     /locus_tag="KLF07_003980"
     CDS             complement(38..400)
                     /locus_tag="KLF07_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD9833840.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="KLF07_003981"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="KLF07_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBD9833841.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="KLF07_003982"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="KLF07_003982"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD9833842.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="KLF07_003983"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="KLF07_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBD9833843.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="KLF07_003984"
     CDS             complement(4827..5036)
                     /locus_tag="KLF07_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833844.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1307 a         1310 c         1380 g         1284 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 t
//
LOCUS       DAEEBB010000214         5281 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEEBB010000214 DAEEBB010000000
VERSION     DAEEBB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.214"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..471)
                     /locus_tag="KLF07_003985"
     CDS             complement(<1..471)
                     /locus_tag="KLF07_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD9833845.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="KLF07_003986"
     CDS             complement(471..1391)
                     /locus_tag="KLF07_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD9833846.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="KLF07_003987"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="KLF07_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD9833847.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="KLF07_003988"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="KLF07_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBD9833848.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="KLF07_003989"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="KLF07_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBD9833849.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1430 a         1203 c         1226 g         1422 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gaacattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DAEEBB010000215         5244 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEEBB010000215 DAEEBB010000000
VERSION     DAEEBB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.215"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..1133)
                     /locus_tag="KLF07_003990"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="KLF07_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="KLF07_003991"
     CDS             complement(1133..1315)
                     /locus_tag="KLF07_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833850.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="KLF07_003992"
     CDS             complement(1327..3060)
                     /locus_tag="KLF07_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD9833851.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="KLF07_003993"
     CDS             complement(3057..3551)
                     /locus_tag="KLF07_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBD9833852.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="KLF07_003994"
     CDS             complement(3673..4023)
                     /locus_tag="KLF07_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD9833853.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="KLF07_003995"
     CDS             complement(4066..4530)
                     /locus_tag="KLF07_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD9833854.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="KLF07_003996"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="KLF07_003996"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBD9833855.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="KLF07_003997"
     CDS             complement(5024..5239)
                     /locus_tag="KLF07_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD9833856.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1148 a         1534 c         1300 g         1262 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggg gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tccc
//
LOCUS       DAEEBB010000216         5114 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEEBB010000216 DAEEBB010000000
VERSION     DAEEBB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.216"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..379
                     /locus_tag="KLF07_003998"
     CDS             <1..379
                     /locus_tag="KLF07_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833857.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="KLF07_003999"
     CDS             complement(575..2125)
                     /locus_tag="KLF07_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBD9833858.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="KLF07_004000"
     CDS             2297..2743
                     /locus_tag="KLF07_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBD9833859.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="KLF07_004001"
     CDS             complement(2937..3872)
                     /locus_tag="KLF07_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD9833860.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="KLF07_004002"
     CDS             4384..>5114
                     /locus_tag="KLF07_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833861.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAEEBB010000217         5044 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEEBB010000217 DAEEBB010000000
VERSION     DAEEBB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.217"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="KLF07_004003"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="KLF07_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="KLF07_004004"
     CDS             1595..1903
                     /locus_tag="KLF07_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD9833862.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="KLF07_004005"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="KLF07_004005"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBD9833863.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="KLF07_004006"
     CDS             complement(3750..4700)
                     /locus_tag="KLF07_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD9833864.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="KLF07_004007"
     CDS             complement(4839..>5044)
                     /locus_tag="KLF07_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HBD9833865.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAEEBB010000218         5029 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEEBB010000218 DAEEBB010000000
VERSION     DAEEBB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.218"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            72..187
                     /gene="rrf"
                     /locus_tag="KLF07_004008"
     rRNA            72..187
                     /gene="rrf"
                     /locus_tag="KLF07_004008"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(305..748)
                     /locus_tag="KLF07_004009"
     CDS             complement(305..748)
                     /locus_tag="KLF07_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBD9833866.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            905..1834
                     /gene="metA"
                     /locus_tag="KLF07_004010"
     CDS             905..1834
                     /gene="metA"
                     /locus_tag="KLF07_004010"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBD9833867.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2103..3704
                     /gene="aceB"
                     /locus_tag="KLF07_004011"
                     /pseudo
     CDS             2103..3704
                     /gene="aceB"
                     /locus_tag="KLF07_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3734..5025
                     /gene="aceA"
                     /locus_tag="KLF07_004012"
                     /pseudo
     CDS             3734..5025
                     /gene="aceA"
                     /locus_tag="KLF07_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1260 a         1317 c         1283 g         1169 t
ORIGIN      
        1 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
       61 aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg ccgaactcag
      121 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      181 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc attggcgcag
      241 aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc acggcagggg
      301 cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc aaatcgtcca
      361 cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc gcctgctcat
      421 tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc tccaccagca
      481 tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc atatgctgcc
      541 cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc cacaacggcg
      601 cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct gctgataaaa
      661 aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc ccttcctcat
      721 gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct tatattctaa
      781 cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat tggcaaattt
      841 tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt gaggtaatca
      901 ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc gtgaagaaaa
      961 cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac ttaaggttct
     1021 gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc gcctgctttc
     1081 aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg aatcgcgcaa
     1141 cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc aggaacaaaa
     1201 ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta atgatgtcgc
     1261 ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca cctcgacgct
     1321 gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc ctaagcaaac
     1381 tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc atgcgcttct
     1441 gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg actttccggc
     1501 agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag aaggggatgc
     1561 atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc ccgaatatga
     1621 tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag acccggatgt
     1681 accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga gctggcgtag
     1741 tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga tcacgccata
     1801 cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg atcgttaagc
     1861 gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa acgcacctca
     1921 gcaagtgaat aaattttatt catattgtta tcaataagtt atcaagtatt tttaattaaa
     1981 atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc tgaacgaaaa
     2041 gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg aggagctgca
     2101 cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg tatggcgagc
     2161 aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg gtgacgcatt
     2221 ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa gatattgata
     2281 acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct gattggaaaa
     2341 ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc ccggtagagc
     2401 gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc gatttcgaag
     2461 attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg cgtgatgcag
     2521 ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc aagcccaatc
     2581 cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat gtcacctggc
     2641 gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc cacaactatc
     2701 aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc cagtcctggc
     2761 aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt aatctgccgc
     2821 gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc cagatggacg
     2881 aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc tgggattaca
     2941 tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca gacagacagg
     3001 ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt aaaacctgcc
     3061 ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc aaagatgaag
     3121 agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa gtcaataacg
     3181 gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg gcggtattca
     3241 acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa gacgcgccga
     3301 ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa ggtatgcgcg
     3361 ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac ggctgcgtgc
     3421 cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc tcgatctggc
     3481 agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa gccttgttcc
     3541 gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa gaacgtttct
     3601 cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact tccgatgagt
     3661 taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc acataactat
     3721 ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga aagagtggac
     3781 tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg tgaaattacg
     3841 cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa tgtggcgtct
     3901 gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga ctggcggtca
     3961 ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat ggcaggtagc
     4021 ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc cggcaaactc
     4081 ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc agatccaatg
     4141 gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc cgatcgttgc
     4201 cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga aagcgatgat
     4261 tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga agaaatgcgg
     4321 tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac tggtcgcggc
     4381 gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta ccgatgccga
     4441 tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta ttaccggcga
     4501 gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga tcagccgtgg
     4561 cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc cggatctgga
     4621 actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac tgctggctta
     4681 taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta ttgccagctt
     4741 ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg caggtatcca
     4801 cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg agggtatgaa
     4861 gcactacgtt gagaaagtgc agcagccgga atttgccgct ataccttcgt atctcaccag
     4921 caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg cggcacgtct
     4981 tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaagcaa
//
LOCUS       DAEEBB010000219         4886 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEEBB010000219 DAEEBB010000000
VERSION     DAEEBB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.219"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..305)
                     /gene="vapC"
                     /locus_tag="KLF07_004013"
     CDS             complement(<1..305)
                     /gene="vapC"
                     /locus_tag="KLF07_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBD9833868.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     "
     gene            complement(305..532)
                     /gene="vapB"
                     /locus_tag="KLF07_004014"
     CDS             complement(305..532)
                     /gene="vapB"
                     /locus_tag="KLF07_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBD9833869.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            614..>4886
                     /gene="traI"
                     /locus_tag="KLF07_004015"
     CDS             614..>4886
                     /gene="traI"
                     /locus_tag="KLF07_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBD9833870.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDHFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRK"
BASE COUNT         1086 a         1277 c         1569 g          954 t
ORIGIN      
        1 gcgatcattt gatcaaatgg cccgacaggg cgtccctgac gggcaagttc tgctcttatc
       61 tggccggtgt gtgtggctgc agcagcgtcg taatccagaa catcaatgcg ggaaacaaat
      121 ccctcgatca cagcgagatt acgttcaggc atctggcttt tttctgcacc atatatcagc
      181 tccatcaggg tgaccgaact gatacacatt ctcccctggt tcaggttaaa acgctccctg
      241 acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt tggtatcgag cataaacttc
      301 agcatcagaa tgactccctt tcctgcatgc cgggctgttc cctgttatcc ataaaatcgg
      361 cgctgacgct gtgtccgtcg aaccattcgt cccatgtctc tccggctggc gtaatgattc
      421 tggttcgtcc gacagcaatc acttcaacgc gttttacgtt ttccggcaat gcaaccgctt
      481 ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac ggtggtttcc attatttgcc
      541 tccttatgtg gatatgtctt gtgtatatct attgtgggat tgatgccggg atatgtcaaa
      601 gggatatacg tttatgatga gtatcgcgca ggtcagatcg gccggaagtg ccgataatta
      661 ttataccgac aaggataatt actatgtgct gggcagcatg ggagaacgct gggccggcca
      721 gggggctgaa caactggggc tgcagggcag tgtcgataag gatgtcttta cccgccttct
      781 ggagggcagg ctgccggacg gggcggattt aagccgcatg caggatggca gtaacaggca
      841 tcgtcccggc tacgacctga ccttctccgc tcccaaaagt gtctccatga tggccatgct
      901 cgggggggac aaacgtctta ttgaagcaca taaccaggcc gtggattttg ctgtccgtca
      961 ggtggaggca ctggcctcca cacgggtgat gacggacgga cagtcagaaa cggtgctgac
     1021 cggtaatctg gtgatggcac tgtttaacca cgacaccagt cgcgatcagg aaccacagtt
     1081 acacacgcat gcggtggtga ctaatgttac gcagcataac ggcgaatgga agacactgag
     1141 cagtgacaaa gtggggaaaa cggggttcat tgagaatgtg tacgctaatc agattgcctt
     1201 tggcaggctc taccgggaaa aacggaaaga gcaggttgag gcgctgggct atgaaactga
     1261 agtggtcggt aagcacggta tgtgggaaat gccgggtgta ccggtggagg ccttttccgg
     1321 acgctcacag actatccggg aggccgtcgg ggaagacgcc tcgctgaaat cccgggatgt
     1381 ggcggcgctg gatacgcgta aatccaaaca gcacgtcgat cctgaagtca gaatggccga
     1441 gtggatgcag acgctgaagg aaaccgggtt cgacatccgg gcatatcgtg acgcagcgga
     1501 acaacgggca tatacccgca cgcagacacc cggacccgct tcacaggacg ggccggatgt
     1561 gcagcaggcg gtgacacagg ccattgccgg attaagtgaa cgcaaagtgc agtttatgta
     1621 tacggacctg ctggccagga cggttggcat actgccgccg gaaaatggtg tgattgaacg
     1681 ggcacgcgcc ggtatcgatg aggccatcag ccgtgagcag cttatccccc tcgatcgtga
     1741 gaaggggctg ttcacgtccg ggattcatat gctcgatgag ctgtcagtcc gggcactcag
     1801 tcgtgacatc atgaaacaga accgggtgac cgtacatccg gagaaaagtg tcccccggac
     1861 ggccggttac agcgatgccg tcagtgttct ggcacaggac cgtccgtcgc tggccattgt
     1921 gtccgggcag ggcggtgcag ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc
     1981 ccgggagcag gggcgggagg tgcagattat cgctgctgac cgtcgctcgc agatgaacct
     2041 gaagcaggat gaacggctgt ccggtgaact gataaccgga cgtcgtcagt tgctggaagg
     2101 catggccttc acgccgggca gtaccgttat cgtcgaccag ggcgaaaaac tctccctgaa
     2161 agagacgtta accctgctgg atggtgccgc acgtcataac gtacaggtcc tgataaccga
     2221 cagcgggcag cgaaccggta caggcagtgc actgatggcc atgaaggatg ccggggtgaa
     2281 cacatatcgc tggcaggggg gagaacagcg accggccacc atcatcagtg aaccggaccg
     2341 taatgtccgc tatgcccggc tggccggaga ttttgcggcc agcgtgaaag ccggagaaga
     2401 gagcgtggca caggtcagcg gagtacggga acaggccata ctgacacagg ccattcgcag
     2461 tgagctgaaa acacagggcg tgctcggaca cccggaggta accatgaccg ccctgtcacc
     2521 ggtctggctg gacagccgga gccgttatct gcgggatatg taccgcccgg gaatggtgat
     2581 ggagcagtgg aacccggaga cacgcagtca tgaccgctat gtgacagaac gtgtgacggc
     2641 gcagagtcac agcctgaccc tgcggaatgc gcagggcgaa acgcaggtgg tgcgtatttc
     2701 ttccctggac agcagctggt cgctgttccg gccggaaaaa atgccggtgg cagacggtga
     2761 gcgtctgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca
     2821 ggtggcatcc gtcagtgaag atgcgatgac ggttgttgtg ccgggacggg ctgaaccggc
     2881 caccctgcct gtgagcgatt caccgttcac ggcactgaag ctggagaacg gctgggtgga
     2941 aacgcccggg cattccgtca gcgacagtgc gacggttttt gcctccgtca cacagatggc
     3001 aatggacaat gccaccctga acggtctggc ccgcagtggt cgtgatgtcc ggctgtattc
     3061 ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct ttacggtggt
     3121 ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta tcagtctgca
     3181 gaaagccggg cttcacacgc cggcacagca ggccattcat ctggcccttc ctgtgctgga
     3241 aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc
     3301 agaaggaacc ggttttgctg acctgggagg ggaaatcaat gcgcagataa aacggggtga
     3361 tttactgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc
     3421 gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc
     3481 gctgatggag agagtacctg gcgaactcat ggagaaactg acatcaggac agcgcgccgc
     3541 cacccgtatg atactggaaa cgtccgacca tttcacggtg gtgcagggct atgccggtgt
     3601 gggtaagacc acacagttcc gggcggtgat gtcagccgtg aacatgctgc cggagagtga
     3661 acgtccccgt gtcgttgggc tgggtcccac gcaccgcgcg gtcggggaga tgcgcagcgc
     3721 cggcgtggat gcgcagacac tggcgtcctt tctgcatgac acgcagctgc agcagcgcag
     3781 cggagaaacg ccggatttca gcaacacgct gttcctgctc gatgagagct caatggtggg
     3841 caataccgac atggcacggg catacgccct gattgcggtc ggtggcggtc gggctgtggc
     3901 cagcggtgac acggaccagc tgcaggccat cgcgcctggt cagcctttcc gtctccagca
     3961 gacgcgcagt gctgccgatg tggtcatcat gaaggagatt gtgcgtcaga cgccggaact
     4021 gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa agggcgctgt ccgggcttga
     4081 gcgtgtgaaa ccgtctcagg tgccacgtct ggagggcgca tgggcaccgg agcactccgt
     4141 gacggagttc agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aggcgatgct
     4201 gaaaggcgag gcttttccgg atgtccccat gacactgtat gaagccattg tccgcgacta
     4261 taccggcagg acaccggaag cacgggagca gacgctgatt gtcacgcacc tgaatgagga
     4321 tcggcgcgta ctgaacagca tgattcatga tgcacgggaa aaggccggag aactggggca
     4381 ggtgcaggtc atggtgcctg tcctgaacac agcgaatata cgcgacgggg agctgcgtcg
     4441 tctctccacc tgggagaata acccggatgc ccttgccctg gtggataatg tgtatcaccg
     4501 gattgccggt atcagcaagg atgacgggct gataaccctg caggatgcgg aaggtaacac
     4561 gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc acgctgtaca ccccggacac
     4621 catccgggtg gggaccggtg acaggatacg cttcacgaag agtgaccggg agcgcggtta
     4681 tgtggccaac agcgtctgga cggtgacagc ggtttccggt gacagtgtca cactttctga
     4741 cggtcagcag acccgtgtga ttcgccccgg tcaggagcgg gcagagcaac atattgacct
     4801 ggcctatgcc atcaccgccc acggtgcaca gggggcgagt gaaacctttg ccatcgcgct
     4861 tgaaggcacg gaaggtaacc ggaaac
//
LOCUS       DAEEBB010000220         4838 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEEBB010000220 DAEEBB010000000
VERSION     DAEEBB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.220"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(263..463)
                     /locus_tag="KLF07_004016"
     CDS             complement(263..463)
                     /locus_tag="KLF07_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833871.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="KLF07_004017"
     CDS             complement(463..735)
                     /locus_tag="KLF07_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD9833872.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="KLF07_004018"
     CDS             complement(738..1016)
                     /locus_tag="KLF07_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833873.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="KLF07_004019"
     CDS             complement(1017..1574)
                     /locus_tag="KLF07_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833874.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..3689)
                     /locus_tag="KLF07_004020"
     CDS             complement(3138..3689)
                     /locus_tag="KLF07_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9833875.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            4488..4784
                     /locus_tag="KLF07_004021"
     CDS             4488..4784
                     /locus_tag="KLF07_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBD9833876.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1364 a          994 c          921 g         1559 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aacccttctc taaggctgat aactgtcaca
     3421 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
     3481 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaatag tctgctgaaa
     3541 ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagtgaa ccatttagca
     3601 atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta
     3661 ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt
     3721 ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca
     3781 ttctaccgtt acgttgaaag tgtctcaaaa ggaagtcctt ttaagactgg ttatgaagga
     3841 gctatgaggt tgattagggg ttggtacaag cccaactaac cgaatcaact atgaagattt
     3901 taggagggta actgtaacca tcactttcta actttcacgt aggaaattgt taagtggttt
     3961 gatagttaca ccatgtgacc aagcatctat atatcgttat atagccctgt atatagcact
     4021 atatgtagtg tcgagagagg aacgactaac acgatacagt ttaccctgaa aagagacttc
     4081 acagattctt tatatatttc aataagttag ggtaaaaagg gactataccg agactgtata
     4141 gaatctgtgt agaattgcat agatacttgg aaatctacta ctaaattgca tagggactca
     4201 cctgatattg tcctgttttt aacaggtctc ctagaaaacc gaatagactt cattcaaggg
     4261 tcattgaagt cataatcatt tcagtagaaa aaccaagtct cgattttaaa tgcaaatagc
     4321 aatcatattc aataaggcaa gacgatcagg atcagtgaaa atgtcatttt tggcgtaatt
     4381 gaccttactt tatgcatggt cttaacaaag acatcatctt tccgttgcag atcttcgccc
     4441 tttcaaataa cgtactttac aactttcccg aacaaggagt tgtgcccgtg ttgtatgtta
     4501 tttacgctca agacaaagct gatagcctcg aaaaacgcct ttccgttcgt ccggcacatt
     4561 tagcacgttt acagttactg catgatgaag gtcgtttgtt gacagctggt ccaatgccag
     4621 cggtagacag taacgatcca ggggctgcgg gatttactgg ctcaaccgtt attgctgaat
     4681 ttgaatcgct ggaagcagct caggcctggg ccgatgctga cccgtacgta gcggcaggtg
     4741 tctatgaaca cgtttcagtg aaaccattta agaaagtgtt ctgaatagag attcaatatt
     4801 taagggcacg gtttttgcaa ccgtgttctt gtttggcc
//
LOCUS       DAEEBB010000221         4803 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEEBB010000221 DAEEBB010000000
VERSION     DAEEBB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.221"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(279..1322)
                     /locus_tag="KLF07_004022"
     CDS             complement(279..1322)
                     /locus_tag="KLF07_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBD9833877.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="KLF07_004023"
     CDS             complement(1734..2918)
                     /locus_tag="KLF07_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD9833878.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="KLF07_004024"
     tRNA            complement(3218..3312)
                     /locus_tag="KLF07_004024"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4803
                     /locus_tag="KLF07_004025"
     CDS             3605..>4803
                     /locus_tag="KLF07_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBD9833879.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1055 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgc
//
LOCUS       DAEEBB010000222         4796 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEEBB010000222 DAEEBB010000000
VERSION     DAEEBB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.222"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            6..81
                     /locus_tag="KLF07_004026"
     tRNA            6..81
                     /locus_tag="KLF07_004026"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KLF07_004027"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KLF07_004027"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KLF07_004028"
     CDS             complement(299..1057)
                     /locus_tag="KLF07_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBD9833880.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KLF07_004029"
     CDS             complement(1065..2168)
                     /locus_tag="KLF07_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBD9833881.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="KLF07_004030"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="KLF07_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="KLF07_004031"
     CDS             complement(3427..4452)
                     /locus_tag="KLF07_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9833882.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAEEBB010000223         4784 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEEBB010000223 DAEEBB010000000
VERSION     DAEEBB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.223"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(179..346)
                     /locus_tag="KLF07_004032"
                     /pseudo
     CDS             complement(179..346)
                     /locus_tag="KLF07_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            495..1370
                     /locus_tag="KLF07_004033"
     CDS             495..1370
                     /locus_tag="KLF07_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HBD9833883.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(1417..1749)
                     /locus_tag="KLF07_004034"
     CDS             complement(1417..1749)
                     /locus_tag="KLF07_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBD9833884.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            1763..4558
                     /locus_tag="KLF07_004035"
                     /pseudo
     CDS             1763..4558
                     /locus_tag="KLF07_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            complement(4601..4784)
                     /locus_tag="KLF07_004036"
                     /pseudo
     CDS             complement(4601..4784)
                     /locus_tag="KLF07_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1173 a         1210 c         1261 g         1140 t
ORIGIN      
        1 tgttatcttg ttatcttgtt atcttgttat cggggtctga cgctcagtgg aacgaaaact
       61 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      181 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
      241 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      301 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttaagg tggttgtaaa
      361 taatgttaca atgtgtgaga agcagtctaa attcttcgtg aaatagtgat ttttgaagct
      421 aataaaaaac acacgtggaa tttagggact attcatgttg ttgttatttc gtatcttcca
      481 gaataaggaa tcccatggtt aaaaaatcac tgcgccagtt cacgctgatg gcgacggcaa
      541 ccgtcacgct gttgttagga agtgtgccgc tgtatgcgca aacggcggac gtacagcaaa
      601 aacttgccga attagagcgg cagtcgggag gcagactggg tgtggcattg attaacacag
      661 cagataattc gcaaatactt tatcgtgctg atgagcgctt tgcgatgtgc agcaccagta
      721 aagtgatggc cgcggccgcg gtgctgaaga aaagtgaaag cgaaccgaat ctgttaaatc
      781 agcgagttga gatcaaaaaa tctgaccttg ttaactataa tccgattgcg gaaaagcacg
      841 tcaatgggac gatgtcactg gctgagctta gcgcggccgc gctacagtac agcgataacg
      901 tggcgatgaa taagctgatt gctcacgttg gcggcccggc tagcgtcacc gcgttcgccc
      961 gacagctggg agacgaaacg ttccgtctcg accgtaccga gccgacgtta aacaccgcca
     1021 ttccgggcga tccgcgtgat accacttcac ctcgggcaat ggcgcaaact ctgcggaatc
     1081 tgacgctggg taaagcattg ggcgacagcc aacgggcgca gctggtgaca tggatgaaag
     1141 gcaataccac cggtgcagcg agcattcagg ctggactgcc tgcttcctgg gttgtggggg
     1201 ataaaaccgg cagcggtggc tatggcacca ccaacgatat cgcggtgatc tggccaaaag
     1261 atcgtgcgcc gctgattctg gtcacttact tcacccagcc tcaacctaag gcagaaagcc
     1321 gtcgcgatgt attagcgtcg gcggctaaaa tcgtcaccga cggtttgtaa tagcggaaac
     1381 ggaatgggga aactcattcc gtttttgttt atcgccttag acggcaaaag tgctgtcgcc
     1441 cacctgcgct tgcgcatacc aggccataag ctccgtggtt cctggttctc cttccgctgg
     1501 agcccagtgc gcatagtcat cggcagccac gggttgatag ccaccgtgtt ttacttcaaa
     1561 aattatgcca ccggtatcca gcgacagcac ggcatgccag gttcctgcgg ccatctccag
     1621 caccgtacag gtttccccca atatcgcccg atgggtgacg gtaccccgat cgtcaaaatt
     1681 cagcaccacg aaacgacccc ttaatggcaa cagtagctcg aaggtgtgag ggtgtcggtg
     1741 cgggcgcacg taggtcccag gtcatattcc ttccggcgtc cggcatttta ccgccagaca
     1801 gctcgggatt cgtgatatca ccgttcttgc agaatacggt cagagggaaa atacccgccg
     1861 tgagcatgca gcgctgatac gtcagcacta tcagtatcgt gaatttgcct ggccctggac
     1921 atttcgcctt acccgtcttt tatatacccg gagctggata agcaacgaac gtcctggcct
     1981 gcttttcgat ctggcgacag ggtggcttat gcaacatcgt attattctcc ccggagccac
     2041 tacgctgacc cggttgattt cagaggtaag ggaaaaggcg acgttgcgcc tgtggaacaa
     2101 actggcactg ataccgtcag ccgaacagcg ttcacagctg gagatgctgc tggggccaac
     2161 tgattgcagc cgcctgtctt tactggaatc actgaaaaag ggccctgtga ccatcagtgg
     2221 tccggcgttt aatgaagcaa ttgaacgctg gaaaactctg aacgattttg gcctgcatgc
     2281 tgaaaacctg agtacactcc cggctgtgcg cctgaaaaat ctcgcacgtt atgctggtat
     2341 gacttcggtg ttcaatattg ccaggatgtc accgcagaaa aggatggcgg ttctggttgc
     2401 ctttgtcctt gcatgggaaa cgctggcgct ggatgatgca ttggacgttc tggacgccat
     2461 gctggccgtt atcatccgtg acgccagaaa gattgggcag aaaaaacggc tccgctcgct
     2521 gaaggatctg gataaatctg cattggcgct cgccagcgca tgttcgtacc tgctgaaaga
     2581 agaaacaccg gacgaatcga ttcgtgctga ggtgttcagc tacatcccaa ggcaaaagct
     2641 ggctgaaatc atcacgcttg tccgtgaaat tgcccggccc tcagacgata attttcatga
     2701 agaaatggtg gagcagtacg ggcgcgttcg tcgtttcctg ccccatctgc tgaataccgt
     2761 taaattttca tccgcacctg ccggggttac cactctgaat gcctgtgact acctcagccg
     2821 ggagttcagc tcacggcggc agttttttga cgacgcacca acggaaatta tcagtcggtc
     2881 atggaaacgg ctggtgatta acaaggaaaa acatatcacc cgcaggggat acacgctctg
     2941 ctttctcagt aaactgcagg atagtctgag gcggagggat gtctacgtta ccggcagtaa
     3001 ccggtgggga gatcctcgtg caagattact acagggtgct gactggcagg caaaccggat
     3061 taaggtttat cgttctttgg ggcacccgac agacccgcag gaagcaataa aatctctggg
     3121 tcatcagctt gatagtcgtt acagacaggt tgctgcacgt ctttgcgaaa atgaggctgt
     3181 cgaactcgat gtttctggcc cgaagccccg gttgacaatt tctcccctcg ccagtcttga
     3241 tgagccggac agtctgaaac gactgagcaa aatgatcagt gatctactcc ctccggtgga
     3301 tttaacggag ttgctgctcg aaattaacgc ccataccgga tttgctgatg agtttttcca
     3361 tgctagtgaa gccagtgcca gagttgatga tctgcccgtc agcatcagcg ccgtgctgat
     3421 ggctgaagcc tgcaatatcg gtctggaacc actgatcaga tcaaatgttc ctgcactgac
     3481 ccgacaccgg ctgaactgga caaaagcgaa ctatctgcgg gctgaaacta tcaccagcgc
     3541 taatgccaga ctggttgatt ttcaggcaac gctgccactg gcacagatat ggggtggagg
     3601 agaagtggca tctgcagatg gaatgcgctt tgttacgcca gtcagaacaa tcaatgccgg
     3661 accgaaccgc aaatactttg gtaataacag agggatcacc tggtacaact ttgtgtccga
     3721 tcagtattcc ggctttcatg gcatcgttat accggggacg ctgagggact ctatctttgt
     3781 gctggaaggt cttctggaac aggagaccgg gctgaatcca accgaaatta tgaccgatac
     3841 agcaggtgcc agcgaacttg tctttggcct tttctggctg ctgggatacc agttttctcc
     3901 acgcctggct gatgccggtg cttcggtttt ctggcgaatg gaccatgatg ccgactatgg
     3961 cgtgctgaat gatattgcca gagggcaatc agatccccga aaaatagtcc ttcagtggga
     4021 cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa gtacaggttt cagtgctggt
     4081 ccgttcattg ctgaaaagtg aacgtccttc cggactgact caggcaatca ttgaagtggg
     4141 gcgcatcaac aaaacgctgt atctgcttaa ttatattgat gatgaagatt accgccggcg
     4201 cattctgacc cagcttaatc ggggagaaag tcgccatgcc gttgccagag ccatctgtca
     4261 cggtcaaaaa ggtgagataa gaaaacgata taccgacggt caggaagatc aactgggcac
     4321 actggggctg gtcactaacg ccgtcgtgtt atggaacact atttatatgc aggcagccct
     4381 ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa gatatcgcac gcctctcccc
     4441 gctttgccac ggacatatca atatgctcgg ccattattcc ttcacgctgg cagaactggt
     4501 gaccaaagga catctgagac cattaaaaga ggcgtcagag gcagaaaacg ttgcttaacg
     4561 tgagttttcg ttccactgag cgtcagaccc ctaaaatcct gatgctgatg tagtcccgtt
     4621 ttatcgtcat cacgaagctt tctgctatgc cattgctttc cgggcttcgc actgctgtcg
     4681 tacaaggctc cagtcccagc aaccgggcga acgccggcac tgttggcaac tgccttccga
     4741 agcgtttttc caccgcaccc agcatgacat cctgcaccgt ttct
//
LOCUS       DAEEBB010000224         4760 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEEBB010000224 DAEEBB010000000
VERSION     DAEEBB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.224"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..279
                     /locus_tag="KLF07_004037"
     CDS             <1..279
                     /locus_tag="KLF07_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9833885.1"
                     /translation="YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
                     NRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            620..2272
                     /locus_tag="KLF07_004038"
     CDS             620..2272
                     /locus_tag="KLF07_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD9833886.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2283..2453)
                     /locus_tag="KLF07_004039"
                     /pseudo
     CDS             complement(2283..2453)
                     /locus_tag="KLF07_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2586..2705)
                     /locus_tag="KLF07_004040"
                     /pseudo
     CDS             complement(2586..2705)
                     /locus_tag="KLF07_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2779..3213)
                     /locus_tag="KLF07_004041"
     CDS             complement(2779..3213)
                     /locus_tag="KLF07_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HBD9833887.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="KLF07_004042"
                     /pseudo
     CDS             complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="KLF07_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1273 a         1151 c         1111 g         1225 t
ORIGIN      
        1 tacacacagc ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg
       61 tatgacaacg cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
      121 aagagctgga aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat
      181 aataatcgac gattgctgga aaggctgggc cacatccctc cggcagaagc agaaaaagct
      241 tattatgctt ctatcggaaa cgatgatctg gcagcctgag ttcacagata aaacattctc
      301 caggaaactt ggggcggttc acgtcagtat cagaccatat taattgctga aactgataaa
      361 catcaaacct tatagaaggg tgaatgctga aagggggcta tggtgatcga cgtgatgaca
      421 acgacaggta agaccgtgac ctgataaacc tgaattgtgt cctagacata aagtccgtga
      481 agggaataag gctcaggtca gcgaatctta tagtggcccg cagctctgac tgcaataagg
      541 ccggatacag agcagcaact actcgtcgat gtcagaacaa cggaagcctt gcaaacgggg
      601 atgcgttcat acaggtacaa tgataccgtt aaaaaaaaac attacgctaa taatgttcac
      661 cctgtcatta ctgacaggaa atcctgctat agcgtatgaa acagataaag tatataaaat
      721 aaccgttctg catacaaatg accaccatgg acacttttgg cgtaacaatc atggtgagta
      781 cgggttatcc tcccaaaaaa ctctggtgga caatatccgg cagaaagtaa taaataatgg
      841 cggaagtgtt ctattattat ctggtggtga tattaacacc ggtgtacctg aatcagacct
      901 tcagaaggct gaacccgata ttcgtggaat gaatctaatt ggttatgatg caatggccgt
      961 cggtaatcat gaatttgata atcctctaaa tatattacgt cagcaggaaa aatgggccac
     1021 atttcctttc ctttctgcta acatatatca aaaaagtaca gggaggcgtt tattcagtcc
     1081 atggaaaatt ttcatccgtc agaatttgaa aattgcagtc attggcttga cgacagatga
     1141 tactgcgaaa acaggaaatt ctgaatattt tactgatatt gagttccgtc aacctgctgc
     1201 tgaggcgagg agtgtcattg atgaacttaa tcagcaggaa aagccagata ttattattgc
     1261 ggcaacacat atgggtcact atgacaatgg cgagtcaggt tcgaatgctc ctggtgatgt
     1321 tgaaatggct cgcagtttgc cgactggctc attagcaatg attgtgggag gacattcaca
     1381 agctcctgtc tgcatggcca gcgataataa aaaacagtgg aattatattc cggggacaac
     1441 ctgtgtacca gataaacaaa atggcatctg gattgtacaa gcgcatgagt ggggaaaata
     1501 tgtcggtcag gctgattttg aattctgtaa cggtacaatg aaactggtta attatcagtt
     1561 acacccagtt aatctcaaaa tgagaattac cagagaagat ggaaaaacgg aattttcttt
     1621 ttacaccccc gaaattactg aagatccaca gatgttatcc ttactgacac cattccagaa
     1681 taaaggcaaa gcacaactgg atgtgaaggt tggggttgtg aacggacgtc tggaagggga
     1741 cagaagtaaa gttcgttttg ttcagaccag tatgggccat ctgattctga gcgctctgac
     1801 agaacgtatt gacgcagatt ttgctgttgt cagtggtggg gaaattcgag attccataga
     1861 gagtggtaac atcacataca aagatatact gaaagttcag ccatttggta atacggtagt
     1921 aagtattgat ttgacgggga aagaagtagc tgactaccta gctacagtgg cacagatgaa
     1981 accggattcc ggagcctacc cacaattttt gaataccagc tttgtagtca aaaaaggaaa
     2041 aatcgaaatg ctaaaaataa aaggtaaatc ggttgacctt aacaaaaaat atcgtatgac
     2101 aacatttagc tttaatgcta ctggtgggga tggctatcca cgtattgata atagacccgg
     2161 atacataaat accggattta ttgatgctga agtacttatc gaatatatcc gggaacattc
     2221 accgctggat gctgcttcat atgaaccaaa aggtgaagtc agttggcagt gataacgtaa
     2281 ttgacgatca tcacgtgttt tttgctgaca gtcgctgtga ctttgtgaaa gtagtctctg
     2341 cggatgtttg cgatatggga atgcagttgc tgtattttgt gtttctgctt ctgccagttg
     2401 ttgctgaatt taaccttgcg gcttaactga tgttggagcg ctgccagctt cttcgtcaat
     2461 ttcgctgatt aaagtttatt cattatggca tgatctgcgt aagcggctcg tcagaaccgt
     2521 attgatattt actaagagct cagatcaact ttccagggca acagatcgcg tacccggttt
     2581 gccggtcagt cctggatatg ctcaatgacg taacgcagcc atttttctgg ctccacattg
     2641 ttcagacggc atgtgccgat cagcgagtac aacaccgccg catgttcgcc accgctatcg
     2701 gaaccccgcc ggagagagtt tcactctccg gctgctcacg gaaccgtacg tgacactctc
     2761 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gggcaaatag
     2821 cttcggatac tgtttcctta ccagcgccag ctttcgctga gccttccggt tcgaaagtct
     2881 gtatttagac tgcatccact taagcaaacg tgactgcatt gcactccaca gtcgatagct
     2941 gaagtttctg gaccagaact ttccatagta ctcaatccag cctctcacta tcgcattgta
     3001 gcgcctcgca aaatccagca aactctcagc tgttgagcga tgtatacgcc acttcttgat
     3061 tgtttctgtt attttgcaca ttgctgcatt tgatgcaccc ggcatacatt ttctgtacaa
     3121 ttcgcctttg aaattcttca gagtacgcac tttgaagtca tatccgagaa aggagaaact
     3181 cgtagcgacg tttctccttt taaacgtgtc aatgtaggcg atattcgtct tccctgcgtt
     3241 caggaccaac ccgacttccg agaagcgcac cgataacctg ttcttcagcc ttgttgcatc
     3301 actcattcgt gaacaatgta ctacaagtcg agtcacccga gggaacctcc ccctctggtg
     3361 ctcacggaac cggacgtgaa cctctcagct catccggctc ttctcattga ggcctgtccc
     3421 catagtaacg gccagtgtgc aaacaacctc gtttcactcc gcgcaacacg ggccagccag
     3481 tggctggccc gtcgtcggtg gcccctaagt cgcttgtgtt tacggggcag ccataacacg
     3541 agtttgcgat cgatttgcct cagcgtcaga tacagtgctg atttataaaa agcaccgtag
     3601 tactttatcc agcctctgat tttcgcattg aacatatgcg ccagctcgta caaggacttg
     3661 tcactgcgat tctcctcacc tcctgacgga ttgcttttcc tgctctggca ctcatcgccg
     3721 gactgaagtt aacgaaggtc ttaccccagc gattcatcga cagacgcggc cggaatgtat
     3781 agcccagaaa gtcgaagctc gtttccgggt agtccccccg tcggtcagca tccttacagt
     3841 aaactattcg tgttttttgc ggatgcagct gcagcccgca ttgcgcaagc cgctgcctca
     3901 gtccacttat cagagcgtca gcctgccact ggctgtgaca gtggcataca acatcatcgg
     3961 cgtacctttc gaagggaacg ccgggaaact gacgttgcat ccacatatca aaggcatagt
     4021 gcaggaacag atttgagagc aggggggaga ttacgccgcc ctgcggcgtt cctcgacccc
     4081 gtgccagcat tgctccatcc ggcatttgca ccggtgcctt cagcctgcgc tcaatgtaca
     4141 tcaccaccca gcgtgcctga gtatgatgct gcactgtctt cagtagcagg tcatgatcga
     4201 tatcatcaaa gaagccttta atatcgactt ccaccaccca gtcaaacttc cagcagcgtt
     4261 tcctcgcctg cgcaatcgcc tggtgtgcgg atttacccgg tcgataacca tacgagtctg
     4321 catggaacag gggttccagt accggctcga gtatctgctt aacgaccatc tgggcgatcc
     4381 ggtccgacac ggccggaata ccgagcggac gtacaccacc gtcagctttg ggaatgtcca
     4441 cacgccttac cgcctgcggc atataactgc cggaagccat tctgttccac agtttgtaga
     4501 gattgtctgt aacattctcg tcaaacccgg ccagtgtctg cccgtcaatg cctgccgccc
     4561 cacggttggc ttttactcgt ttataagctt cccatacctg tcgtttactt acaggaaatg
     4621 gctttgccga ttttacagca gtcctcccat cggcattgtc gcccgtggtt gccgccgcac
     4681 catcagcaca atggggcaag ccctttgctc cggacccatt acggtccttc accgctacta
     4741 cggtttactc cgtccctgta
//
LOCUS       DAEEBB010000225         4699 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEEBB010000225 DAEEBB010000000
VERSION     DAEEBB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.225"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            387..1469
                     /locus_tag="KLF07_004043"
     CDS             387..1469
                     /locus_tag="KLF07_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBD9833888.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="KLF07_004044"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="KLF07_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBD9833889.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="KLF07_004045"
     CDS             complement(2127..2267)
                     /locus_tag="KLF07_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBD9833890.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="KLF07_004046"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="KLF07_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBD9833891.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="KLF07_004047"
     CDS             complement(2623..2913)
                     /locus_tag="KLF07_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD9833892.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="KLF07_004048"
     CDS             complement(2906..3076)
                     /locus_tag="KLF07_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833893.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="KLF07_004049"
     CDS             complement(3076..3531)
                     /locus_tag="KLF07_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HBD9833894.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="KLF07_004050"
     CDS             3796..4173
                     /locus_tag="KLF07_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833895.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1351 a         1048 c          977 g         1323 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaaa ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggtagcaatt tatatactac ttaatcttgt atacattatt gctcacccat tatttcctga
     4381 aaaatatcta aaaacatcaa catctatatt tctattcatt tccttcatat taagtgcaat
     4441 agtagcatat tttgttttcc ataagcataa atctgccact ctatccatat gcagcttagt
     4501 gttcttagtg ttaattgtag gtgctaacgt agggtttatc agttacagcg acctaacaca
     4561 aaaagcgacg caaaaattag atagtttgtc taaataatta tgttgccatg cgaagtatgc
     4621 atggctgcat gtctgccttc catttaaaat gggctaagac ctataaccct aaatattatt
     4681 ctttattatc ttctttacc
//
LOCUS       DAEEBB010000226         4654 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEEBB010000226 DAEEBB010000000
VERSION     DAEEBB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.226"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(101..608)
                     /gene="aqpZ"
                     /locus_tag="KLF07_004051"
                     /pseudo
     CDS             complement(101..608)
                     /gene="aqpZ"
                     /locus_tag="KLF07_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            complement(727..2505)
                     /locus_tag="KLF07_004052"
     CDS             complement(727..2505)
                     /locus_tag="KLF07_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBD9833896.1"
                     /translation="MWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILAS
                     TLSLVVISYSLSIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAG
                     ESGRQLLPALMQAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILL
                     AMPSVSKSQRQKVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVA
                     PDAKLMAENITGKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELS
                     ALCDKEVLNVPVIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVR
                     NNVFGTLYCAESAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTR
                     FCMVRFGNVLGSSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMG
                     HGGDVFVLDMGDPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEEL
                     LIGDSVQGTSHPRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFN
                     PTDDICDLVWQQKKSLLSQASNVIRL"
     gene            complement(2661..3251)
                     /locus_tag="KLF07_004053"
     CDS             complement(2661..3251)
                     /locus_tag="KLF07_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBD9833897.1"
                     /translation="MKRIFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVG
                     RFGKDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTM
                     SLVGPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYID
                     IILPIKQRYYLDYVANNSVKYDCVIIWKTIIKILSR"
     gene            complement(3268..4422)
                     /locus_tag="KLF07_004054"
     CDS             complement(3268..4422)
                     /locus_tag="KLF07_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBD9833898.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYLNEISIPLYTKMTDQDQLFVIKSIRQLFM"
BASE COUNT         1528 a         1089 c          841 g         1196 t
ORIGIN      
        1 gaggatcgcc atgatttggc tgtcggtaaa ttttgatgtt ttcataaaga atctcctgcg
       61 tttatggtac gagaaaattc tacttttgac gctggttaat ttacggggtg attaccagac
      121 caatcgccag tggcgcaaag cccgcaggag cgcgtttttc tgtcgctccg gacaaaaaac
      181 gcactgagga caaactcgct cagcatacag gcgcttaacg catagccgcc aggtgaatgg
      241 aggccataac cattagccgc aaaaccgctg gttgtcgcat caaacccagc cttaccgctg
      301 gcgataccat acagcactgc cgcagcggca ataccgccga taacctgagc gataatgtaa
      361 ggtaacacgc gcgctgctgg gaagcgtcca ccggcccaca gacccaaggt caccgcgggg
      421 ttaaaatgcg caccagagat gtgcccgacc gcataagcca tggtgactac tgtcagacca
      481 aacgccaacg ccacgcccaa aaagcctata cctaactgtg ggaaagcagc agagatcaag
      541 gcgctaccac agccacccag aaccagccag aaagtgccga aaaattcggc gctaaaaggt
      601 ttaaacatac actgatgtcc tgtttgttaa agagcaggca tcagggtaaa ggcggtcacg
      661 atgtaccgcc tagcggggat ttgtaagaga ggtgaagaat tggtgtggaa ggttaaacct
      721 aagcaatcac aggcgaatga cattgctcgc ttgtgataac agcgattttt tctgctgcca
      781 aactagatcg caaatatcat cagttggatt gaatgccgct ggtgcttgta acaacaaact
      841 gcgaattcgc tcatgatcaa agtcatgaca agcttgatcc agctctttaa gtaagagcga
      901 tagatcctgc cacggtagca tcacttcgtt ggccgtcata attcgtggat gagaggtacc
      961 ttgaactgaa tcaccaatca gtaattcttc atacagtttc tcacctggac gtaatcccgt
     1021 aacttcaatg gcaatatcgc catctggatt tttatcatcc cgtacactca agccacttaa
     1081 ccggatcatg cgtttggcta agtcataaat cttgaccgga tcgcccatgt ctaagacaaa
     1141 gacatcgccg ccatgcccca tcgcccccgc ttgaatcacc aactgtgatg cttccggaat
     1201 tgtcatgaaa tagcgaataa tgtcacgatg agtcaaggta actggcccac cttgggcaat
     1261 ctgtttttca aacaacggca cgacagagcc cgaagaaccg agtacattac caaatcgcac
     1321 catacaaaag cgagtttggc tttgccgtgc agacaaagcc tgcaatacca attcggccag
     1381 acgcttagtt gtccccatag tgttggtcgg gcgcaccgct ttatcggtgg aaatcaacac
     1441 aaaagtttca acgccacttt cgatcgctga ctcagcgcag tacaaggtac caaacacgtt
     1501 attacgtacc ccttccacca cattatgctc aaccagaggc acatgtttat aagcggccgc
     1561 atgataaacc gtttgaatac caaaggactt catcaccatc tgtaagcgat tctgacgctg
     1621 caccgagccc aacagaggga tcactggaac attcaaaact tctttgtcac acagcgccga
     1681 gagctctttc tcaatagcgt agagggcata ttcagacagt tcaaatagaa ccaatttggc
     1741 cggcttatat cgaacaattt gacgacaaag ctcagagccg atcgagcctc ccgccccagt
     1801 gaccataacg gctttgccag taatgttttc ggccatcaat ttggcatcag gagcaaccgg
     1861 atcacgacct agtaagtcat caatcgatac tttttttaga ttactgattt gtgctcgacc
     1921 ttcgactaaa tcgaccatac ccggaataga gagaacttca cacggtagat gctctaaacg
     1981 agtaatcact ttctgccttt gtgacttact gacgctcggc atcgccaaaa gaattttctt
     2041 tataccatag cgatctacaa ggtaactcag tttatccgag ggataaactg ttacaccatg
     2101 aatgacagcc ttatgcaagc gaggattatc atccacaaat gccacaggaa aatattcttt
     2161 tgcctgcatt aatgctggca ataattgtcg gccagattca ccagcgccat aaatcaaaac
     2221 aggcgcacta cccttaactc catagttaag tatcattcta aaaagcaatc tagagcctga
     2281 tgtcagtaaa agtaaaacca aaaaataaat tccgacaaca gtgcgcggca acattatgga
     2341 tagcgaatat gatataacga caagagataa cgttgaggcc agaataccaa ctggtattag
     2401 caacattatc tttgcactaa cataccgtaa gactgcccga taaagcccaa tcctgataaa
     2461 tataaacacg gtaaatatgg taagaatagc tgcagcaaac cacatcggtg cactaagaaa
     2521 agccgtttgc tcatcaagcc tcaaccaata agacaaccaa aatgagaata tcaccataac
     2581 tacatcaatg cagatgatga tacctctctt aacaattctt ggcagctcca atattctatc
     2641 aatcatccta cactacctta ttatcgcgac aaaatcttaa taatagtttt ccaaattatc
     2701 acacaatcat actttactga attgttagca acataatcta aataatatct ttgcttgatt
     2761 ggaagaatta tatctatata agccctacgt gggtcatcat aactagacaa aatctcattt
     2821 tcatctacca tttctataga tgctaagtca gttatccctg gcctaaccga taaaactttt
     2881 tcccttatat catcaggata ctcatcaata aactccctca cttccggtct tgggccaacc
     2941 aaactcattg ttccagaaag aacatctatc aattgaggaa gctcatcgat tttgtacttc
     3001 cgtaaatacc atccaactct ggttacccga gcatcttgac caactgttat ccgtcctttt
     3061 ttttctgaat cgataaacat cgttctaaat ttatgtatcc taaaatcttt cccaaatctc
     3121 ccaactctat actgcctaaa aaaaaccccc cctttagaat cagcaacaat taacattgac
     3181 acaatgataa aaacaggaaa tagaaaaagc aggcctaagc ctgccacgat aacatcaaaa
     3241 atacgtttca tttaatataa aataccatta cataaataat tgtctaatcg atttgataac
     3301 gaacaactga tcttgatccg tcattttagt ataaagagga atagatattt catttaaata
     3361 acactcctca gaaactggaa agtcactggc gttcaaatta tatgtatcac gccaaaccgg
     3421 ttgcttatgc aacggtataa aatggacaga acaaccaatt ccaagatctg ataacttctt
     3481 aataaaatca tcgcgattaa tagccgaatc agtttttaag cggataggat atagatgcca
     3541 agcatgaata tcactagcat tagtaggcca ttcaggcaat tcaagtggca attcttttaa
     3601 cgcatcatcg taaatttttg ccattcgttg acgttttttc tgaaaatcat cgatctttct
     3661 aagttgatga ataccgattg ccgcacagat atcaggcata ttgtatttaa accctggagc
     3721 tacaacctca taaaaccaag aaggagtttt agactggtac cggtcaaaag cgtcacgact
     3781 gattccatgt aaacgcatta ccttacaacg ctcaattata tctttatttt ttgaaacaac
     3841 cattccgcct tcaccggttg tcatagtttt attggcgtag aagctaaaaa ccgtagcatc
     3901 tgaatcaagc gttcctatct tacttccttt atatgttgta ggaaatgcat gagcggcatc
     3961 ctcgacaacc tttaggtcat attttttagc aattgataag attgaatcca tgtcacaagc
     4021 taatccagcg aagtgtactg gaataatcgc ctttgtttca ttagtaatag ccttctcaat
     4081 ggcatcaact gatatattaa atgttttacg atctacatca acaattacag gatcagcacc
     4141 aaggtacctg acaatttcgg cagtagcagt gaatgtatat gatgggacaa taacttggtc
     4201 tcccggcttt acgccaacag cttcaagagc caaatgtaag cccgacgtag cagagttaac
     4261 agctaatgat tgaacgttcg ctcctaggta attagaaaat tcttgttcaa attgcttagc
     4321 cttaggacct gtcgtaatcc aacctgaacg taaagagtca attacctctg caatttcttc
     4381 ttcgccaatt tcaggtaacg caaaaggaat aaaatttttc atttaaaata ccttttcaaa
     4441 aaagataata acgatccaca tccataagaa atatgataag acaaaaatat aaaaggaaga
     4501 aatactctac ctataaaact tcgcccatct cgaattgaaa acaaaataga aataagaaaa
     4561 taaaaaaata aaaaagataa atatatataa tcaccagtag caaagaaaag agaaaaagat
     4621 acaattaaat acaaaacaaa aaaaagtggc acta
//
LOCUS       DAEEBB010000227         4607 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEEBB010000227 DAEEBB010000000
VERSION     DAEEBB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.227"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..2162
                     /locus_tag="KLF07_004055"
     CDS             <1..2162
                     /locus_tag="KLF07_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBD9833899.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="KLF07_004056"
     CDS             complement(2396..3679)
                     /locus_tag="KLF07_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBD9833900.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..4506)
                     /locus_tag="KLF07_004057"
     CDS             complement(3814..4506)
                     /locus_tag="KLF07_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD9833901.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1178 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgct
//
LOCUS       DAEEBB010000228         4482 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEEBB010000228 DAEEBB010000000
VERSION     DAEEBB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.228"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(7..1068)
                     /locus_tag="KLF07_004058"
     CDS             complement(7..1068)
                     /locus_tag="KLF07_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBD9833902.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="KLF07_004059"
     CDS             complement(1065..2462)
                     /locus_tag="KLF07_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBD9833903.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="KLF07_004060"
     CDS             2617..3615
                     /locus_tag="KLF07_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD9833904.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="KLF07_004061"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="KLF07_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBD9833905.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAEEBB010000229         4461 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEEBB010000229 DAEEBB010000000
VERSION     DAEEBB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.229"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..258)
                     /locus_tag="KLF07_004062"
     CDS             complement(<1..258)
                     /locus_tag="KLF07_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833906.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTP"
     gene            487..1095
                     /locus_tag="KLF07_004063"
     CDS             487..1095
                     /locus_tag="KLF07_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBD9833907.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1193..2584
                     /gene="selA"
                     /locus_tag="KLF07_004064"
     CDS             1193..2584
                     /gene="selA"
                     /locus_tag="KLF07_004064"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBD9833908.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2581..4425
                     /gene="selB"
                     /locus_tag="KLF07_004065"
     CDS             2581..4425
                     /gene="selB"
                     /locus_tag="KLF07_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBD9833909.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1000 a         1134 c         1319 g         1008 t
ORIGIN      
        1 gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca gcggggcggg
       61 cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat tgaccggatg
      121 gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg caccgatgcg
      181 caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc cctgacgcgc
      241 tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc tgcttccagg
      301 caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat agaaacaatt
      361 aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa caaaccatca
      421 acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc gcacaaagga
      481 ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact ttctattctg
      541 ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa cgcggacaac
      601 ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa
      661 tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt cgctccggcg
      721 atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc gctggcggat
      781 ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc ggcgcagcag
      841 tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa
      901 ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac tatcgccatt
      961 gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc
     1021 ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa
     1081 ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac aatccgtggt
     1141 tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc ctatgacaac
     1201 cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc gcgatagctc
     1261 cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt tgcgtcagat
     1321 gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt ggtgtgaaaa
     1381 ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt
     1441 gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac aggcggaagc
     1501 cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt acgatctgga
     1561 cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc gtattacggg
     1621 ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga tgttggcggc
     1681 cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga ttggcggcgc
     1741 gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag tagggaccac
     1801 caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg cactgttgat
     1861 gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag atgaagcgga
     1921 actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag gcagtggctc
     1981 gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg agttgattgc
     2041 ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg
     2101 gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc
     2161 attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc tttatttaca
     2221 ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca gcgcagaggt
     2281 cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg gcgcggagtt
     2341 tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc cggttgatcg
     2401 cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc accttgagtc
     2461 attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt atgacggacg
     2521 attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga tgttgttgaa
     2581 atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt gcaggtgatt
     2641 actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac catcgatctc
     2701 ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga cgtacccggt
     2761 catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca cgcgctgttg
     2821 gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc gattttgcag
     2881 ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg
     2941 cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag
     3001 gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat
     3061 ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct cgcgatcgac
     3121 cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt aagcggggaa
     3181 gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg
     3241 ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac
     3301 atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct tgccgatgcg
     3361 ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc gctgacccag
     3421 tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg
     3481 gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga taacgatcgc
     3541 ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt
     3601 aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc gtctcttgca
     3661 cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc ggttaacctt
     3721 gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag
     3781 cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc ccgctggcag
     3841 cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga acctggtcct
     3901 gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct ggtactgttg
     3961 ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg gctgcatctg
     4021 ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa agcagagccg
     4081 ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg taccgacgag
     4141 caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac cgcgatcgtc
     4201 aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc
     4261 gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga
     4321 aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga
     4381 aatgatcatt tattgcgcga cgcattatta tttccggaaa aataaggaaa tgattaatta
     4441 atttttaaaa taaattaata c
//
LOCUS       DAEEBB010000230         4413 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEEBB010000230 DAEEBB010000000
VERSION     DAEEBB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.230"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..590)
                     /locus_tag="KLF07_004066"
     CDS             complement(<1..590)
                     /locus_tag="KLF07_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBD9833910.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="KLF07_004067"
     CDS             complement(583..993)
                     /locus_tag="KLF07_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HBD9833911.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="KLF07_004068"
     CDS             complement(999..1532)
                     /locus_tag="KLF07_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HBD9833912.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2604)
                     /locus_tag="KLF07_004069"
     CDS             complement(1510..2604)
                     /locus_tag="KLF07_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HBD9833913.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLVLGENILSCDTERSIRERFSEYQVSGQR
                     PGNDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALT
                     RETTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRV
                     GPADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2601..3992)
                     /locus_tag="KLF07_004070"
     CDS             complement(2601..3992)
                     /locus_tag="KLF07_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HBD9833914.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4039..4245)
                     /locus_tag="KLF07_004071"
     CDS             complement(4039..4245)
                     /locus_tag="KLF07_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833915.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1058 a         1289 c         1042 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc accagcgccg tcgccgcttt acctgttccg ggttttccca gaaccagtcg
     2101 cccgcattca tcgtcataag ccagcgtctg aacctgcccc agtaaccggt taagacaatc
     2161 ggcaacggtt tcgccgtgtt ccggctgggc atcaatcacc gctgtctgcg gcacgccagc
     2221 atcaacaacg gtgatgccaa atggcgcagc cagttcactg actattctga gcaggttttt
     2281 tccgctctgc tggagtggca aagcagagca gtcaaccaga tcggctgttt tgcttcgccc
     2341 gacaattccc atgctgacgc tgctggcgtc ataacgaagc ggtagtgcct ccacatatcc
     2401 ggtgagcaca ggctcatccc cgataagcac ttcaaccagc tcaccatttt ttatccgggg
     2461 ttgataatcc cggcttccgg gccagcgggt ggtaatggca acattaaaat cccgggcaat
     2521 acggttaatg cccgcactga tacggacgga tgtccagccg ccccattcgc gaccggaaac
     2581 ccgaagtaaa acggtatcat tcatctgacg ggtaccctta atgccctgac cggaacaaag
     2641 cccggatgag agatggcatt tcgatccagg atatcagttt cacgggacgc atcgtcgtac
     2701 catgacgcag ccagaacaag cgcaggcaga acttccgctg gcgttcgctc tgcggtttcc
     2761 tccgtctgaa ccagacgcgc ctgaatatcc cggttcagtt ctgtacgtaa tgacgtcagc
     2821 tgaaaaaaca gcctgtcatc cgccgtacgc ctgagctcct gttcaatcgc cgcgttcagc
     2881 gatttgcgta tgatggtgag attttcccgc gtgggtggtt gcgctctctc atcctgtcct
     2941 gtgctggctg ccacgctgtc aagtgccgga tgagaaacgt gaataatatc ggactgacgt
     3001 tcagtggaac caccaacagc cacaaccgcc tgctgatttt tcaccaggct tccgggttgt
     3061 ggcagtgaag tgaccgccct tgtggcttcg ctgattgccg tcgtccggat gacagcagcc
     3121 acaaggtttg tctgctgttt ttgcctgata accgatgcgg aatctgtggg ccacaccgca
     3181 cgcggtgcca gtcctgggtc cagcgtaatg ccggacattg tcgttatgga ctgcaccaga
     3241 tcctgtgtgt tatccaccag ccgggttccg gcccgccagg tatcctgcaa cgtatgcaca
     3301 aaatcactgg caaccgacgg cggcatcaga atgacggata aatcgccctg cagtagtctc
     3361 gctccggcag aaacagccga attaaccatt ttgaaagctg tctgaacggt tcccagcata
     3421 tcggtcgtcc gggcaataac atcgttctga ataaagtccg gtattcccgc gagatcaaaa
     3481 tcgccgaaca tgtcttcaat caactcatcc aggaatacgg acgactcctc cagttttctg
     3541 gcggttgcag ctccggcgac cggaaatgac agttccccgc tctcaacaaa ctgaaatgaa
     3601 acccggcaca tacggccttc agtgccggag tgagaaacgg tcacctgccc gtcaatacag
     3661 ccctgcattt caccgaactg cggatggatc agcgtccccg gcccggcggt ttcaatcgct
     3721 gtaatcagcc tgtcgcgctg ctctgcgtaa tcatcaccaa cgagatacgc attaatcgtc
     3781 agccgtcgcg tggcacgccc gagatcttcc gtgtacggtt tgtcacggtt tgggtattca
     3841 tggacctgaa cgcggcgtcc gaacgaaccc tcgtcgctct ccaccgaaaa cggaacgcca
     3901 cgaaatgaag cgtcgtaaag attatcgcgc caattgatac ctgaagatgc agaaaagagt
     3961 gaagaaagag aaggtaaaga aggtaagtcc atactgtctc cactataaaa aaaccgccaa
     4021 tttctggcgg cttatatatt aatcatcatt aatatctttc atattcaatt tgcaattttc
     4081 gtaggtcact cccgactcgc cattaacatt aactccagct ctgatattcc cgttttcttt
     4141 tacatctaca aaaaactggc ctcttggcat cttgaacgca aatgtaaaac caacagtatc
     4201 tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat gtcatcgtcg ctggtgatgc
     4261 attgtttgca aaatcaaata cacctatact tttgccgaat tcataagaag cttttctcaa
     4321 tataatcgca ccgctggcag aaggggatgg gcacgatatc ctcagttgtc tgaatccatc
     4381 tgtatccaag tcaacgtcat tatctgcaga tgt
//
LOCUS       DAEEBB010000231         4409 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEEBB010000231 DAEEBB010000000
VERSION     DAEEBB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.231"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(173..2122)
                     /locus_tag="KLF07_004072"
     CDS             complement(173..2122)
                     /locus_tag="KLF07_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD9833916.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="KLF07_004073"
     CDS             complement(2207..2542)
                     /locus_tag="KLF07_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBD9833917.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="KLF07_004074"
     CDS             complement(2542..2898)
                     /locus_tag="KLF07_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBD9833918.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="KLF07_004075"
     CDS             complement(2898..4394)
                     /locus_tag="KLF07_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBD9833919.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1313 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcctttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAEEBB010000232         4409 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEEBB010000232 DAEEBB010000000
VERSION     DAEEBB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.232"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(21..1958)
                     /locus_tag="KLF07_004076"
     CDS             complement(21..1958)
                     /locus_tag="KLF07_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBD9833920.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2369..3076
                     /locus_tag="KLF07_004077"
     CDS             2369..3076
                     /locus_tag="KLF07_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBD9833921.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="KLF07_004078"
     tRNA            3182..3257
                     /locus_tag="KLF07_004078"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="KLF07_004079"
     CDS             3455..>4409
                     /locus_tag="KLF07_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD9833922.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAEEBB010000233         4399 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEEBB010000233 DAEEBB010000000
VERSION     DAEEBB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.233"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..699
                     /locus_tag="KLF07_004080"
                     /pseudo
     CDS             1..699
                     /locus_tag="KLF07_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1452
                     /locus_tag="KLF07_004081"
     CDS             706..1452
                     /locus_tag="KLF07_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD9833923.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1467..1889
                     /locus_tag="KLF07_004082"
     CDS             1467..1889
                     /locus_tag="KLF07_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD9833924.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1947..2303
                     /locus_tag="KLF07_004083"
     CDS             1947..2303
                     /locus_tag="KLF07_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833925.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2472..3137
                     /locus_tag="KLF07_004084"
     CDS             2472..3137
                     /locus_tag="KLF07_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBD9833926.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3365..3520
                     /gene="hokD"
                     /locus_tag="KLF07_004085"
     CDS             3365..3520
                     /gene="hokD"
                     /locus_tag="KLF07_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD9833927.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            3770..4057
                     /locus_tag="KLF07_004086"
     CDS             3770..4057
                     /locus_tag="KLF07_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833928.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            4096..>4399
                     /locus_tag="KLF07_004087"
     CDS             4096..>4399
                     /locus_tag="KLF07_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD9833929.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
BASE COUNT         1193 a          972 c         1212 g         1022 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagagga agaggaagag gaagaggaag atctaaatac
      361 tgatctcccc ctcgccaaaa acgagcgtct aaaaaattcg agccggaggc tattgagctg
      421 cccgattggt tgccggaaac actctggcat gagtgggtcc ggttcagaca ggcattgcga
      481 aaaccgattc gaacggagca gggcgctaac ggggcgatac gggaactgga aaaattccgt
      541 cagcagggtt ttacacctga gcaggtgatt cgacacagca tcgccaatga ataccagggc
      601 ctgttcgcgc cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg
      661 ttgccggaca gtgcgatccc gccaggcttc agggggtaac ggaccatgaa aaatattgcg
      721 acaggcggcg ttctggaacg catccgccga ctgaccccgc cacatgtaac cgccccattc
      781 agaacggtag cggagtggcg cgagtggcaa cttgctgaag gccagaaacg tagcgaggag
      841 atcaaccgcc tgaatcgcca gttgcgggtg gaaaaaattc tgaatcgctc aggcatccag
      901 ccgttgcacc gtaaatgctc gtttgcgaat taccaggtgc agaacgacgg ccagcgatac
      961 gcgttaagcc aggcgaaatc catcgccgat gaactgatga ccgggtgtac aaattttgcg
     1021 ttcagcggaa aacctggtac cgggaagaat cacttagcgg cagctatcgg gaatcgcctg
     1081 ctgaaagacg gtcagacagt gattgtggtt accgtggctg atgttatgag cgccctgcac
     1141 gccagctatg acgacgggca gtcaggcgaa aaatttttgc gggagctgtg cgaagtggat
     1201 ctgctggttc ttgatgaaat tggcattcag cgcgagacga aaaacgagca ggtggtgctg
     1261 caccagattg ttgatcgccg gacagcgtcg atgcgcagcg tggggatgct gacaaacctg
     1321 aactatgagg tgatgaaaac attgctcggc gagcgggtga tggatcgcat ggtcatgaac
     1381 ggcgggcgct gggtgaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
     1441 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
     1501 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
     1561 tgtcagatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
     1621 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
     1681 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
     1741 atccggacct tatctggtca ttacctgacg gagaaatacg ccgctacgac aggcgcctga
     1801 atataatctg tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
     1861 agggtaattt tcaggaggcg atactgtgaa tgaaattagc tatcaggctt caattaccgc
     1921 tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag ttaagcaata
     1981 tcgttttatt tccggtaaaa gaggatgacc ctcgtaatca ggttaatttt ctttatgagc
     2041 catcggaaag accatattgc catcacgcct ctgtacgggt tgacgaaaaa gagcgtcagg
     2101 tccgctgtaa aatctgcggt gcggttgtgg agccgtttga ctggatgctc tctgtggcaa
     2161 aaagagaaac cagactggca gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc
     2221 ggagaaatat agaaaagctg atacagattg agcgtaacgc gaaagcgcgg atacgcaggg
     2281 cgacaaaatc cagaactgaa taattaaatt tagcactgtt aaaaatttaa tccttaaccg
     2341 gagggatttc tgcaccctca aatcatcagg agaccacccg aaagggcggg gagcagtcac
     2401 acatctgttt ccgatagccc cgttctaatg ctacactctt tgatattttt atgaccccaa
     2461 taaacatatt tatgacagtt gctgatttca aacggcccaa attggagctc ccaaacgggg
     2521 caaacaaact actactgcac tcttgctgtg ctccatgttc cggtgaggtg atggaggcgc
     2581 ttcaggcctc gggaatcgac tacaccatct ttttctacaa cccgaacatt catcctcaga
     2641 aagagtattt aattcgtaag gatgagaata ttcgctttgc tgaacaacac ggcgtgccgt
     2701 ttattgatgc tgattacgac acagacaact ggtttgaacg agccaaagga atggaatggg
     2761 agcccgaacg agggatccgt tgcaccatgt gttttgacat gcgttttgag cggacagcgc
     2821 tgtacgccgc tgaaaatggt ttcagtgtga tcagcagttc actgggcatt tcacgctgga
     2881 aaaatatgca gcaggttaac gactgtgggc ggcgagccgt cgcgcattat ccgggcatgg
     2941 tgtactggga ttataactgg cgcaagcagg gcggctcgtc ccgtatgatt gaaatcagca
     3001 agcgcgaaaa attctatcag caggaatatt gtggctgtgt gtattctctg cgcgatacca
     3061 atctacaccg caaatctcag ggacgccctc ttatcaaaat tggtcaactc cactacggta
     3121 aagaagagaa ggagtgattt tatggggcac ctttctgatt gatttcatat tggcgaggta
     3181 agtagaatga ctgcgggtgc ttgaggctat ctgcttcagg catgaacacc aaaaggcaga
     3241 tagagaaaag ccccagttaa cattacgcgt ccggcaagac gcttaacatt aatctgaggc
     3301 caatttcatg ctttgcacat gtaggttagc ctcttacatg ccgaaaggca aggagaagca
     3361 ggctatgaag cagcaaaagg cgatgttaat cgccctgatc gtcatctgtt taaccgtcat
     3421 agtgacggca ctggtaacga ggaaagacct ctgtgaggta cgaatccgaa ccggccagac
     3481 ggaggtcgct gtcttcgtag actacgaatc caggaagtaa gagtgaccgg gcggggagct
     3541 gatcccatcc ccgcccacct ctgatgtgtc aggcatcctc aacgcacccg cacttaaccc
     3601 gcccatcgct gtgatctctc agcgtttcgg cgggtttttt gttgtttatt tccggtgaat
     3661 ttgattcgcg caccttcgca gatagaatcg actcacttaa gtagcgcgca gggagaagag
     3721 ggatggaccc cgaacagggg agagctattt atctggaagg attctgaaga tgaaaatcga
     3781 agaattgcgt gaaattttta gtgaaaatgg cctctatgct gtgcgcgttg agaatggagc
     3841 gattgtcagc cattgccgca ttagatgttt gcaatctcaa caaaggaaga gcggtgctgt
     3901 gttattttat ttttgtaatg gacttctgac ggacggtttt attttgcgtg aggacgaatt
     3961 tgtcacatca ttacgggttt tgaaagagat tggttttaag gctggttttc tgcttttgct
     4021 gaagaataaa ctcatctaca atcttgagca ggattgaact cctgctgtgt aacaccgtgc
     4081 caccggagaa agccgatggc acatatacaa ctggtcaaac aaacctcttc cggattactt
     4141 ctcccggcga cgccggagag ttgcgatttt ctgcatcaaa tcaaaatagg tgagtggata
     4201 cacgcagact ttaagcgtgt gtgtaactac gcattccaca agcgtttttt caaactcctg
     4261 caactgggtt tcgattactg gactccggtc ggtggggcga tcacgcctcg cgaacgaaaa
     4321 ctggtgtccg gtttcgttga ttacctgtgt gaatcagtag gccgggaaca tacgccagct
     4381 ctgagcgaag ccgcagagc
//
LOCUS       DAEEBB010000234         4393 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEEBB010000234 DAEEBB010000000
VERSION     DAEEBB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.234"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..396)
                     /locus_tag="KLF07_004088"
     CDS             complement(<1..396)
                     /locus_tag="KLF07_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBD9833930.1"
                     /translation="MIDAGGTFLLKAIFQIGVFVYFTHVSDITTFGIISYVFTVYWFV
                     LNFSDYGFRTKLVKDISDNSYSASELLSRSDGVKTYVFFFIFIIFMFYSYVSDSISLT
                     LLVYISSAYFVCISSGRFSLLQAVGRFRCE"
     gene            complement(497..1522)
                     /gene="tviC"
                     /locus_tag="KLF07_004089"
     CDS             complement(497..1522)
                     /gene="tviC"
                     /locus_tag="KLF07_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosaminuronic acid C-4 epimerase TviC"
                     /protein_id="HBD9833931.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGY
                     IYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRLSMPW
                     YVRFLKG"
     gene            complement(1615..2895)
                     /gene="tviB"
                     /locus_tag="KLF07_004090"
     CDS             complement(1615..2895)
                     /gene="tviB"
                     /locus_tag="KLF07_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HBD9833932.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFFAGYSPERINPG
                     DKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEAAKVIENTQRDV
                     NIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQS
                     VGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGLTFKENCPDLRN
                     TKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYDAIIIAVAHNEF
                     REMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            complement(2964..4070)
                     /locus_tag="KLF07_004091"
     CDS             complement(2964..4070)
                     /locus_tag="KLF07_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBD9833933.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALPGAMFAIVGTLVYATLKDKTKLD"
BASE COUNT         1428 a          886 c          665 g         1414 t
ORIGIN      
        1 ttcacatcta aaccgaccaa cagcctgtag caagctaaat ctaccacttg aaatacaaac
       61 aaaatatgca gatgaaatat aaacaagcag agttaatgaa attgaatcag aaacataaga
      121 ataaaacatg aagattataa aaatgaagaa aaaaacatat gttttaactc catcacttct
      181 tgataataat tctgatgcag aataactatt atcagaaata tctttcacta attttgttct
      241 aaatccataa tcagagaagt taagcacaaa ccaataaaca gtaaacacat aactaataat
      301 accaaatgta gtaatatctg acacatgtgt gaaataaaca aaaactccta tttgaaatat
      361 tgctttaagt aaaaatgtac cacctgcatc aatcattttt ctaaacatac actgaccatt
      421 tagaataaat acatggattt tcacccctgc attaataaca gaggtgaaat agattattta
      481 ttcatgttaa tataatttag ccttttaaaa atctcacata ccacggcatt gaaagtcgta
      541 atccctctct gatttttata tttggtctat actttagtaa atctatagcc ttagtaacat
      601 cagcctgaga atgcctaaca tctccagatc taaactctct atacttaatg ctcaatttat
      661 cgatatggtg aattaaatta agctcatcat aaatgtaacc agataattca tttaacgttg
      721 ttctatcacc aactgcaaca ttatatatat tatctttagc actgtccttc gctaatgcag
      781 ataatatatt catttgtata acattatcta tataacaaaa atcacgactc gtttcaccat
      841 cgccattaat atatacgtca tcacctttaa gcattgctgc tgtccatttt ggaattactg
      901 cagcataagc tccattagga tcttgacgac gaccaaatac attaaaataa cgtaatccaa
      961 tagttttaaa accatatgtt cgagcatata cctgagcata aatctcgtta acatatttag
     1021 taactgcata aggagaaagt ggattaccaa tgttttcctc tacttttggt agtgcgggat
     1081 gatctccata agttgagctt gatgcagcat aagtaaaact ttgtacttgt gcatttttag
     1141 ccgcatgtaa gatattcaaa aatccagtaa tattagttgc attggttgtt ataggatcaa
     1201 caattgaacg aggtacagaa cctagcgcag cctgatgtaa gacatgatca acacctttca
     1261 taacttgctc acaggtagtg agatctcgaa tatcaccttc tataaagcaa aatcgactcc
     1321 actgttcagt ggaaactaat gttttaactt catcaagatt atattgatgt cccgtggaaa
     1381 agttatctaa cccaataaca acctggttta atttaagtaa cttttctaaa agatttgatc
     1441 ctataaagcc agcgacacca gtaattaacc aagttttcgg tgaaaaaatt aactgctgtg
     1501 taatctcttc atagcgagac atataaatat ccatgtatat taatcaagtg ctaaacctag
     1561 cataaaatgc tgaggtgtat catatcaaag aggatttgat ttttttaatc actcttacaa
     1621 gcgcatatcg atactttttt tatcaagcac atattttaaa tcgaacaaaa cgtgctcgtc
     1681 tttacctaat gcacgaatag cgctctctcc catctcgcga aactcattgt gtgcaacagc
     1741 gataattatt gcatcataat gattaacttt aggatcttca cataaagtta atccatattc
     1801 atgttgtgcc tcatcggtag aacaccatgg atctataata tctatattga tattgtattc
     1861 ttttaactct gaaataatat caatcacttt agtgtttcgt agatctgggc aattctcttt
     1921 aaatgtaagc cccatcacta acacattcgc cccttcaact tgaatccgtt gtttcaacat
     1981 ttttttgact aactgggaaa cgacatactg ccccatacta tcatttaaac gacgtccggc
     2041 taaaatcatc tccggatgat agccgacact ttgcgcttta tgtgtaagat aataaggatc
     2101 tacacctata cagtggccac ctactaaacc gggcctaaaa ggtaaaaaat tccacttcgt
     2161 acctgcagcc tcaagaacct ctaaggtatc aatccctaac ttattaaata taatagataa
     2221 ctcattaatc aatgcaatat tgacatctcg ctgcgtgttt tcaattactt ttgcagcctc
     2281 tgctactttt atcgatgatg ctttatgcgt accgacagta attattaatt tatatagctg
     2341 atctacatac tcagcaacat ccggtgtaga tccactggtc accttaagga tattagttac
     2401 acgatgctct ttatccccag gattaatacg ctcaggtgaa taaccggcaa aaaaatcaat
     2461 attaaactta agacctgata ctttctctac aactggtata caatcttctt ctgtcgctcc
     2521 agggtaaact gttgactcat aaataataac atcgcctttc tttattatct tacccaatgt
     2581 ttcagatgct ttaattagag gtgttagatc tggctgttta tgtttatcaa ttggagttgg
     2641 aacagttaca ataaatacat tacactcttt tagtgcatct aatgaacagg cgtacgtcaa
     2701 tttagttgct tctaacaact ctaaatttga gcactctaat gtactatcgt gaccatttcg
     2761 taattcatca atacgagact tattaatatc aaatccaatc gtcgttactt tctttccaaa
     2821 ctcaacagca agaggcaatc caacataacc aaggcctata atcccaattt tcgcattcaa
     2881 agtatcgaat ttcattttct tatccatcat aaagtgcaca tagaacatct atttcgcatt
     2941 gacacaacaa tacgtaaccc agtttaatct aacttggttt tatcttttaa tgtcgcataa
     3001 actaatgtac caactatagc aaacattgcc cctggtaatg caccaagaat caaaattaat
     3061 gctttgttgg gtttgtcttt tataactggc tcagatggct tcatttgata tcgatatgct
     3121 tgcgcctgaa tgttatcgcg taacattcct tctaactcat caatttgacg tttcacttcg
     3181 taatatctaa atgggtaaat aattggcgca tctttagttg cttttagctc tgctgataac
     3241 agtttttcgc cgagcaagaa cttatagcta ctttgcgcat tatcgaagcc gcctttgcta
     3301 gagcgataat caatgattcc ggcttgttcc gcagttgtta aatctcgctg aatatgagct
     3361 atttgatcaa gcttttctga ttttctctgg aaatctatgt caataatttc tttatttaga
     3421 ttaaccaaaa gagtattaac attttgacca aactcatcaa gacttaatgt tacagcttga
     3481 ttattaacat tctttagata tccctgcagt agtttatatg cctcttgcgc tgagtctgcc
     3541 gatgcttgta ttatgtagta acttgtattt cccttttcct ttggtggcgt aatcacaaga
     3601 cctttattcg cttgctcagc taaccaaatt ttcttatctt gttcagtaga taaattattt
     3661 gtttcctttt tataatactc agtactctct agaaatttaa ctttggtgtc tggcgattca
     3721 gcaatcaaaa tcaaatcgga aaacaatcca ttagccaacg cattcgtatc taacggttga
     3781 tttaatattc gggcatacgc ttggtgaaat ttaagatagc tgcctaattc agcaatacgt
     3841 ggtgcttgaa tataggtttt agccgtccac tgctcttttg cggtaaaggc atacactgct
     3901 gctaaccccg tagtcagcaa cgtgacacat aaaataaccc attttttctt ccacaaggtg
     3961 cctaaaagct cgaaaagatc gatttcatcc tctgcttttg gcatataagt tggatagacc
     4021 ccttgcggga tcagataagg ggtctgttgt ggttcagatg cttttgacat gaaaccaatc
     4081 cattatttat caatgggtta aaaataaaaa tgaagaataa gcctgacaaa aaagtacgct
     4141 accgcccttg agtttcagct cccaaagcgc tgcgcagaaa caggatatag ccaactgcga
     4201 tacaacaagt gatatctggg attagaggac ataatcgaga acactccgtg tgaagagaca
     4261 tatacagtcc cacacttccc tgctgcaaac cggcggatag cccgcagaat ttggagtgca
     4321 gattataaag caatgcctgt ataagaccaa atctgccttc aaaatgataa cagtatgttt
     4381 gtaaattctg tga
//
LOCUS       DAEEBB010000235         4326 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEEBB010000235 DAEEBB010000000
VERSION     DAEEBB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.235"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..579)
                     /locus_tag="KLF07_004092"
     CDS             complement(<1..579)
                     /locus_tag="KLF07_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD9833934.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="KLF07_004093"
     CDS             complement(642..1091)
                     /locus_tag="KLF07_004093"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBD9833935.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="KLF07_004094"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="KLF07_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1090 c         1167 g         1042 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagca taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactgt
//
LOCUS       DAEEBB010000236         4289 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEEBB010000236 DAEEBB010000000
VERSION     DAEEBB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.236"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            224..1066
                     /locus_tag="KLF07_004095"
     CDS             224..1066
                     /locus_tag="KLF07_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD9833936.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1069..2157
                     /gene="capU"
                     /locus_tag="KLF07_004096"
     CDS             1069..2157
                     /gene="capU"
                     /locus_tag="KLF07_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBD9833937.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2162..3112
                     /gene="virK"
                     /locus_tag="KLF07_004097"
     CDS             2162..3112
                     /gene="virK"
                     /locus_tag="KLF07_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBD9833938.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            3177..4121
                     /gene="lpxM"
                     /locus_tag="KLF07_004098"
                     /gene_synonym="msbB"
     CDS             3177..4121
                     /gene="lpxM"
                     /locus_tag="KLF07_004098"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD9833939.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         1225 a          799 c          980 g         1285 t
ORIGIN      
        1 tcaggtagcc aaattcagtg tgccacctca aggttcttag tgagtcgttt ttaaaagata
       61 tgataatata tcattgttta tgaattgttg atgtttaatt tagtaaatta tatcaaatat
      121 gccatgggtt tgctatgtta ttcatccagt atctggaaat aaaatgcacc agctttattg
      181 ggttttcctt ttgtctctgc ttactaggtg ccagaaactt taaatgttaa acgaaggggg
      241 cattttgttc aaagccaatc atgttccggt attaatgtat catcatgtca gtcattgccc
      301 cggactggtt acactttctc ccgtgacatt ccggaagcaa atgaaatggt tggctgaaaa
      361 caactggaaa acactgtcat cagatgaact ggagtttttt tatcggggcg gaaaactccc
      421 ccggaaaagc gttatgttga catttgatga tggatacctt gacaactggt ttcaggtata
      481 tcctttatta aaagagttta atctcaaagc acatattttt ttaattaccg gttttattgg
      541 gaatggtccg gttcgtcact ctcctggaaa ggaatattct catcgcgatt gcgaacatca
      601 aatagcgaca gggaatgctg ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt
      661 gcaatctggt ctggtcgaat ttcatgttca tacgcatact catacacgct gggataaaaa
      721 gttcagttca cgagaagaac agtgtaaaca tcttcggcag gacttgttga gcgggagaga
      781 atatttgaaa gaaatgacag gaaaatgcag taaacatttg tgttggccgg aaggttatta
      841 taacaaggat tatattcagg tcgctgaaga acttggattc tattatttat atacaacaga
      901 aagaagaatg aatgctcctg ctaaaggaac tacacggata ggaagaataa gcaccaaaga
      961 acgggaaagt tgcgcatggc tgaaaagacg acttttttat tatacaacac cgtttttttc
     1021 atcgttgctg gcctttcata aggggccaag attaccggat gattaataat gaatatacta
     1081 tttacggaat catcaccgaa tataggtggt caggaattac aggctgttgc tcaaatgaag
     1141 gccctgaaga aaatggggca ttcagttctg cttgtctgca gggaaaacag caaaattgct
     1201 tttgaagcca gtaaattggg aattgatatc acattcgcgt tatttcgaaa cagtcttcac
     1261 atccctactg catggagatt actcggaata gttcatggtt ttcagcccaa tgcaatcgtt
     1321 tgtcacagtg ggcatgatag caatattgtt ggtttagtac ggttattcac ttggaaacat
     1381 ccattcagaa ttatcaggca aaagacatat ttgacacgaa aaacaaaagt tttttcaata
     1441 aatcattttt gcgatgaggt gattgttccc ggaacaagta tgaagacaca tctggagcag
     1501 gaaggatgtc gaacccgggt tactgttgtg cctccaggct ttgatttcca gaaattatac
     1561 gttgattccc gaaacagttt gcctccaaat gttctttctt ggctggcgtc ccgaagggga
     1621 tgccccgtta ttgctcaggt aggaatgttg cgcccggaaa aagggcacga atttatgttg
     1681 aatttactgt tccatttaaa aatgaatgga cgacagttct gttggttgat tgtggggtct
     1741 ggttcgcctg aactgcggga gcatttacag tatcagattg acagtatggg catgcatgat
     1801 gatgttttta ttgctgacaa tgtttttcct gccgcccccg tatatcgggt tgccagtctg
     1861 gtggttctgc cttcagaaaa cgaatcgttt ggtatggtac tggcagaagc atcggcattt
     1921 tctgtgcctg tactggccag tcagattggt ggaatccctg atgttattca gaacaaccag
     1981 accgggacat tgttaccagc aggtaataag cacgcatgga tgtgcgccct gaatgatttt
     2041 tttaatgacc ctgggcgttt ttatcagatg gctcgccagg caaaacagga tatagaagag
     2101 cggtttgata ttaataaaac tgcgttaaaa atactcacat tagcgaagca caagtaacaa
     2161 tatgttttct gtaagtaact tatcatttat cggtttcctt aaaaggattg ttttttcctc
     2221 agattcactc ccggggaagt gggaacacag aaaattccgg ttcatgtaca ttttgcgatg
     2281 tgctataaat ccggttgcca gtattcgata ttattacgaa ctgcgttcct tgcagtgcat
     2341 tgaggatatt ctggcaatac aacccacgtt gccagcaaga attcatcgac cttatttgca
     2401 taaagggggg cgagcctggt cccgggggca atatattctt gagcattatc gctttgtgca
     2461 aaatttgcca gaaaaatatt ctgaattttt gtttccacaa aaatcggtat cattagtcca
     2521 attcatcggg aaagatggag aggattttga tatccagtgt tctcccagtg gttttgaccg
     2581 ggaaggagag ttgatgttgt cattgttttt caataaaata gtgattgccc ggttaacatt
     2641 ttcagtcatt ctgactcaaa atggacacac cgcttttatt ggcggattac agggagcccc
     2701 caaaaatacc ggacctgatg ttattcggtg cgcaacccgg gcctgttatg ggcttttccc
     2761 aaaacggatt atttttgaag cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg
     2821 tctggcagtc agtgagcaca gtcatgtttt ccggcaattg agatactggt atcagaaacg
     2881 caaaaccttt gttgctgtct acagtgattt ctgggagtct gtagcaggga aaacctgtgg
     2941 tgactggtac aaattaccaa cacaggtggt tcgaaagccg ctaagcaata tagctagtaa
     3001 aaaacgctct gagtatcgaa aacgatacgc tttgttggat tatatccatg aaactgctat
     3061 ccgctctttg gatgcatatc ctgtaaactc agaacatcag gacttaaatt aaggtttgat
     3121 gtattctctg aataaaatat taatgatgat tttggtaggg gcattcgcac taaataatga
     3181 aaaaatacaa atccgagttt attcctgaat ttaagaaaaa ttatctttcc cctgtttact
     3241 ggtttacatg gttcgttttg ggaatgattg caggtatttc aatgtttccc ccttcattca
     3301 gagatcctgt cttggccaaa atagggcgtt gggtgggcag attgagcaga aaagctcgtc
     3361 gcagggcgac gattaattta tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa
     3421 taattgtcga caatatgttt gccacagcat tgcaatccat agtgatgatg gcagaactgg
     3481 caattcgtgg tccggaaaag ttccagaaac gagtgttctg gaaaggcctc gaaattcttg
     3541 aggaaatccg gcacaataac agaaatgtga tttttctggt tccccatggc tggagcgtgg
     3601 atattcctgc aatgttgctg gcagctcagg gggaaaaaat ggccgccatg tttcatcagc
     3661 aacgaaatcc agtgattgat tatgtctgga attctgtacg gcgtaaattc ggggggcgct
     3721 tacattcccg ggaggatggg ataaaaccat ttattcagtc agtacgccag ggatactggg
     3781 ggtattacct tccagatcag gatcatggtc ctgaatacag tgaatttgct gatttttttg
     3841 cgacctataa agcgacatta ccaattattg gacgtctgat gaacatcagt caggctatga
     3901 ttataccgct tttcccggtt tatgatgaaa aaaaacattt cctgactatt gaagttcggc
     3961 caccaatgga tgcatgcatt gccagcgcgg acaataaaat gattgcccga caaatgaaca
     4021 aaacagtgga aattttggtg gggtcacatc cggaacagta tatctgggtt ttaaaattgt
     4081 taaaaacgcg caaatcaaac gaagcggacc cgtacccttg aaattataaa gtacaggtat
     4141 ttccactagt tgtttcttac aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc
     4201 cgtttggcac ggaggactgt gtagatgcag ttcgttaaat ttaatttatg cctaaatagc
     4261 tgcgcttaat accgctacac ttttgccag
//
LOCUS       DAEEBB010000237         4256 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEEBB010000237 DAEEBB010000000
VERSION     DAEEBB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.237"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            108..215
                     /locus_tag="KLF07_004099"
     CDS             108..215
                     /locus_tag="KLF07_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD9833940.1"
                     /translation="MTLAELGMAFWHDLAALVIAGILASMIVNWLNKRK"
     gene            591..698
                     /locus_tag="KLF07_004100"
     CDS             591..698
                     /locus_tag="KLF07_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD9833941.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(785..2464)
                     /gene="bcsG"
                     /locus_tag="KLF07_004101"
     CDS             complement(785..2464)
                     /gene="bcsG"
                     /locus_tag="KLF07_004101"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBD9833942.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2461..2652)
                     /gene="bcsF"
                     /locus_tag="KLF07_004102"
     CDS             complement(2461..2652)
                     /gene="bcsF"
                     /locus_tag="KLF07_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBD9833943.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2649..4220)
                     /gene="bcsE"
                     /locus_tag="KLF07_004103"
     CDS             complement(2649..4220)
                     /gene="bcsE"
                     /locus_tag="KLF07_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBD9833944.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1088 a         1067 c         1107 g          994 t
ORIGIN      
        1 ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gccattcttg aacctgaaca
       61 actcaagaat agccgcgaaa cgttgtcatt acaacacagg cggctatatg acgctcgcag
      121 aactgggcat ggccttctgg catgatttag cggctctggt cattgctggc attcttgcca
      181 gtatgatcgt gaactggctg aacaagcgga agtaacgtgt catgcgggcg tcaggctgcc
      241 gtaatggcaa tttgcgcccg gaccaggccg caggggtgaa actctgcggc ctttttcgta
      301 agaaaaatgc aacgattgca cgcggataat attctgcgga atgcgcagca cagagtgaag
      361 acgtgacgga ttccggaaca gaaaaacagt cgtagaatca gcggcaagcc ggaaaggttc
      421 cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa
      481 aacccccgca cgttgcaggt atgcacctga caacatcacg ggggctaatc ttgaacccaa
      541 acaactcaag aatagccgcg aaacgttggc attacaacac aggcggctat atgacgctcg
      601 cagaactggg catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg
      661 ccagtatgat cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct
      721 gccgtaatgg caatttgcgc ccggatcagg ccgcagaggt taaactctgc ggcctttttc
      781 gtgcttactg cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac ggtttatcct
      841 ggtattgaat aactactgca tttgagttct cggagaccgg tgctgtttgt ggcaacccac
      901 tggtgagttt tttccagtca acattgtctt cggtgaaaat cttgccatca agaacgcgaa
      961 ccaccagatc ggagatagct aggaagctgc tcggttggtc aatgacaatc ggtgccccct
     1021 gatgtggtgc cttcatgccg aagaatttca ccccaacggg gacgtctgtg atagacgggc
     1081 tagggatatc acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt
     1141 ccggcaccac gaccaccatc actttacgac ccgatttctc cagttcagta aagaaggcgt
     1201 ccagttcatc aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg
     1261 gataatggtt gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta cggctgtttt
     1321 tatctttttc ggtaacgtcc agccagcggt taagcacggc ggtatcgtca taaaccggcg
     1381 aaccatcaaa gcccagcaaa ataaccggca gatttgtttg atccatcaat tcagtctgca
     1441 tgccgccatt ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca
     1501 tcaggtgctg ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag tcgttatttg
     1561 ccggttgata cagattagtg tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg
     1621 ccgccgggcc actgtaggag gtggcggagt taaagttctt gaactcaata tcgaaatgcg
     1681 accacagtgg atgcgacatc aacccggcgg cttctatatc cgaccaggaa agcgaacaga
     1741 tgttaatcac cagtagttca aatggctgag catcagcggg cagcgaagac gggaaggtcg
     1801 atttacgttt cgcctccgcg ttatagaaat tattcagcca ggcgttaagg ttcgccgttg
     1861 ttggcggtgc agtttgtgcg ggcatatcac ccactaccga tgcgccaccc gtcgccgcaa
     1921 cggttgccgc tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc
     1981 acaaggagaa acttggtccc gccagggtaa gtacgttcag ccatagcagt atggcaacca
     2041 caaaaacggt aatgcgaatc cattgtgaca ggaataacca ggccactaat aaaacaaaaa
     2101 tggccccaat catctgccag ttaataaagc gtgtgacaag gtcgattaaa taatcggtac
     2161 tgaaccccgc cacctgcgaa ccctggctca ttatgctttc cgggccaggc aaccaggtgt
     2221 catgccagaa caaagcaaag ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc
     2281 tgtagcgcgg aatgggcatc agcagaaacg tggcaaacac caaattgagg agcggatgga
     2341 agttaagata tcccgcccac aacaggccga acttaaccag aaaatagaag ttccagccgg
     2401 aaaggccgcg ccagtattgc cagagggaag aaggcatggc ggtattttgc gtaaattgag
     2461 tcattttttg gttgccctgg ctttttccgt gcggcgtaac gtcccggccg gtttaacata
     2521 acgaggttta gcaaagaata aacgtaaggt gtcgcgaatg cgtcgcaaaa agtgccgcgc
     2581 cagatagccc agcgggaaaa atatcagtgc gcaaacgaca ataatttcaa tgatatcgct
     2641 gatggtcatc atgatgagcg ctccacagca tcatctaaca gtcgcatggg ttctggtatt
     2701 cgtcgccagt ggcgaccatc gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa
     2761 ggcagcggca tgccccattg ttctggggca agcaagcgca tctgcaccag ctcggcactg
     2821 atttgatcat cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg
     2881 aaaatatgat tcaacgcggt atccagatcg ttaatccgac agaatgagag aaacagcacc
     2941 agccgattac cgccaatggt catgatatcg ccggtacggt tagggcgaca cagcgtcagg
     3001 gcttgttcaa cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg
     3061 gcaggtaata gagggttatt catcatgttg ttgacggcat tacagaacac atcccacttc
     3121 tggaaaccac gcagtttgag cggctgggtc attgacagca aggtagtgat atcttccggc
     3181 acatagcgac taaacttctg cccttgcacg ctttcgatca tcgtcagaca acgggagagt
     3241 ggcgcattcc acggaataac catatttgca ccgcaggcca ataacaaacg ttcatcggtg
     3301 gcgcgcaggc tagcggtatt ttcccgcacg aggattttca tcgcactacc gcgctggcga
     3361 cgcagggtat gaatgctgcg ggccagtggc tcgatttgcg cattttgctg taaagaaaag
     3421 accaccgtcg ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt attgttgaac
     3481 agttgccagt gttccgatag cggcggcgca ccttccagta cagcaacatt actcagaatg
     3541 cgtttttcgt cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa tgtccagata
     3601 ccattttgtt gctgaacgct aagctgctga cgggcgctga cccctttttc gttgcaccag
     3661 aaggcaatat ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag accaaaaagt
     3721 gaacggtatt cctcaagcaa caatgaaaat tgtttatcgt tattatttcc gggattaatt
     3781 accaaaagcg aacaatggtt taacctgctc catttattca ttttatccaa ccatgagcga
     3841 agccgctcgg caggaatgtt ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa
     3901 ttatggggat caatagaaca ctgcaaatca cgggccaaat agtatagacc tttttcatga
     3961 tttagcattg aaaataattt tattttttcc ggcccttgag aatcatctaa ttgaaagatt
     4021 ttcgccggat cgctgtccat gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca
     4081 attgtttgat tcgccagact gatagcatct tcatggcgat cgacgttaaa ccaccagacg
     4141 ccgcctgctg gcatatgtcg cagctcatcc cataatgatg agataccgat agagaataca
     4201 gggtccacaa tgtccctcat gtcaccattt tgtctgatta tcagtttact agcgaa
//
LOCUS       DAEEBB010000238         4246 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEEBB010000238 DAEEBB010000000
VERSION     DAEEBB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.238"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="KLF07_004104"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="KLF07_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD9833945.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="KLF07_004105"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="KLF07_004105"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBD9833946.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="KLF07_004106"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="KLF07_004106"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBD9833947.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4246
                     /locus_tag="KLF07_004107"
     CDS             4086..>4246
                     /locus_tag="KLF07_004107"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBD9833948.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGG"
BASE COUNT         1048 a         1079 c          980 g         1139 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaat
//
LOCUS       DAEEBB010000239         4198 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEEBB010000239 DAEEBB010000000
VERSION     DAEEBB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.239"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1361)
                     /locus_tag="KLF07_004108"
     CDS             complement(<1..1361)
                     /locus_tag="KLF07_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBD9833949.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="KLF07_004109"
     CDS             complement(1426..1545)
                     /locus_tag="KLF07_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBD9833950.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="KLF07_004110"
     CDS             complement(1700..1918)
                     /locus_tag="KLF07_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9833951.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="KLF07_004111"
     CDS             complement(2272..3750)
                     /locus_tag="KLF07_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HBD9833952.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKIITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1184 a          919 c          738 g         1357 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgattttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtg
//
LOCUS       DAEEBB010000240         4192 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEEBB010000240 DAEEBB010000000
VERSION     DAEEBB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.240"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KLF07_004112"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KLF07_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD9833953.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="KLF07_004113"
     tRNA            complement(330..405)
                     /locus_tag="KLF07_004113"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="KLF07_004114"
     tRNA            complement(412..486)
                     /locus_tag="KLF07_004114"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="KLF07_004115"
     tRNA            complement(603..687)
                     /locus_tag="KLF07_004115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="KLF07_004116"
     tRNA            complement(696..771)
                     /locus_tag="KLF07_004116"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="KLF07_004117"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="KLF07_004117"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBD9833954.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="KLF07_004118"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="KLF07_004118"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBD9833955.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="KLF07_004119"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="KLF07_004119"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBD9833956.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAEEBB010000241         4185 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEEBB010000241 DAEEBB010000000
VERSION     DAEEBB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.241"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..324
                     /locus_tag="KLF07_004120"
     CDS             <1..324
                     /locus_tag="KLF07_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833957.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="KLF07_004121"
     CDS             551..1816
                     /locus_tag="KLF07_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9833958.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="KLF07_004122"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="KLF07_004122"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBD9833959.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="KLF07_004123"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="KLF07_004123"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBD9833960.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4185
                     /locus_tag="KLF07_004124"
     CDS             3627..>4185
                     /locus_tag="KLF07_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833961.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT         1092 a          938 c         1004 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattc
//
LOCUS       DAEEBB010000242         4131 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEEBB010000242 DAEEBB010000000
VERSION     DAEEBB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.242"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            115..528
                     /locus_tag="KLF07_004125"
     CDS             115..528
                     /locus_tag="KLF07_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBD9833962.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="KLF07_004126"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="KLF07_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBD9833963.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="KLF07_004127"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="KLF07_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="KLF07_004128"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="KLF07_004128"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBD9833964.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="KLF07_004129"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="KLF07_004129"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBD9833965.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="KLF07_004130"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="KLF07_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBD9833966.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="KLF07_004131"
     tRNA            complement(3794..3881)
                     /locus_tag="KLF07_004131"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1101 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa a
//
LOCUS       DAEEBB010000243         4076 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEEBB010000243 DAEEBB010000000
VERSION     DAEEBB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.243"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..784
                     /locus_tag="KLF07_004132"
     CDS             <1..784
                     /locus_tag="KLF07_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD9833967.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="KLF07_004133"
                     /pseudo
     CDS             797..1757
                     /locus_tag="KLF07_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="KLF07_004134"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="KLF07_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBD9833968.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="KLF07_004135"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="KLF07_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBD9833969.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAEEBB010000244         4045 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEEBB010000244 DAEEBB010000000
VERSION     DAEEBB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.244"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="KLF07_004136"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="KLF07_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBD9833970.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="KLF07_004137"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="KLF07_004137"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBD9833971.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="KLF07_004138"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="KLF07_004138"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBD9833972.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="KLF07_004139"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="KLF07_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBD9833973.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1142 c          970 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAEEBB010000245         4003 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEEBB010000245 DAEEBB010000000
VERSION     DAEEBB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.245"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            168..503
                     /locus_tag="KLF07_004140"
     CDS             168..503
                     /locus_tag="KLF07_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9833974.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="KLF07_004141"
     CDS             496..978
                     /locus_tag="KLF07_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833975.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="KLF07_004142"
     CDS             complement(1012..1092)
                     /locus_tag="KLF07_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833976.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="KLF07_004143"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="KLF07_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="KLF07_004144"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="KLF07_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="KLF07_004145"
     CDS             2697..3188
                     /locus_tag="KLF07_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBD9833977.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..3981
                     /locus_tag="KLF07_004146"
     CDS             3523..3981
                     /locus_tag="KLF07_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBD9833978.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1495 a          644 c          646 g         1218 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tcgatggtta gactggcccc ctgaatctcc aga
//
LOCUS       DAEEBB010000246         3959 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEEBB010000246 DAEEBB010000000
VERSION     DAEEBB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.246"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..242)
                     /locus_tag="KLF07_004147"
     CDS             complement(<1..242)
                     /locus_tag="KLF07_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD9833979.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="KLF07_004148"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="KLF07_004148"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBD9833980.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="KLF07_004149"
     CDS             2255..3904
                     /locus_tag="KLF07_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD9833981.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAEEBB010000247         3950 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEEBB010000247 DAEEBB010000000
VERSION     DAEEBB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.247"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="KLF07_004150"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="KLF07_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBD9833982.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="KLF07_004151"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="KLF07_004151"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBD9833983.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="KLF07_004152"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="KLF07_004152"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBD9833984.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..>3950)
                     /gene="araH"
                     /locus_tag="KLF07_004153"
     CDS             complement(3554..>3950)
                     /gene="araH"
                     /locus_tag="KLF07_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBD9833985.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT         1063 a          948 c          885 g         1054 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctttt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga
//
LOCUS       DAEEBB010000248         3949 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEEBB010000248 DAEEBB010000000
VERSION     DAEEBB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.248"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="KLF07_004154"
     CDS             complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="KLF07_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBD9833986.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLF"
     gene            1005..1541
                     /gene="sutR"
                     /locus_tag="KLF07_004155"
     CDS             1005..1541
                     /gene="sutR"
                     /locus_tag="KLF07_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBD9833987.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1614..3575
                     /gene="rlhA"
                     /locus_tag="KLF07_004156"
     CDS             1614..3575
                     /gene="rlhA"
                     /locus_tag="KLF07_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBD9833988.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3667..3897)
                     /locus_tag="KLF07_004157"
     CDS             complement(3667..3897)
                     /locus_tag="KLF07_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBD9833989.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1007 c         1001 g          918 t
ORIGIN      
        1 caaacagacc tgcgagcaaa tagaaaatgc ctgcaacggc agctgccagc caacgttgat
       61 ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc gcaataccga
      121 cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat acaattaatg
      181 gcgaaacagg agccgaatat ccagctgctt tcattgctgc gatacccggt gcgttttgcg
      241 atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca aacgaaaaat
      301 caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac
      361 cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg
      421 caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt
      481 ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg
      541 caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga
      601 ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt agtccctgca
      661 atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat
      721 accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc
      781 cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg
      841 aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaatag
      901 aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt
      961 tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc
     1021 gctttttatc cactacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac
     1081 tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca
     1141 gtccgacggt cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat
     1201 ttatttctcc gccgcagtcc gctatgcctt cagtttatga tccacaacag caggcgatgg
     1261 tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga
     1321 tggcatctgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg
     1381 tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg
     1441 gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa
     1501 ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca
     1561 acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg
     1621 tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag
     1681 ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata
     1741 atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg
     1801 caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac
     1861 ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga
     1921 ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta
     1981 cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag
     2041 agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct
     2101 ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa
     2161 cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga
     2221 aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg
     2281 atcagactgc caacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag
     2341 ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg
     2401 atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt
     2461 tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg
     2521 cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg
     2581 aagtattgaa agtggcgaaa gatcatctca atgttgccgt caccgagcca ctggcaaatg
     2641 gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg
     2701 agaaaaccgg agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca
     2761 aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga
     2821 caaaaacctc cagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac
     2881 aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg ctggacgggc
     2941 agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat ggtctggcaa
     3001 aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg ggggcgctgt
     3061 ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg ctggatgctg
     3121 cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct gcgccggttt
     3181 ttccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg cgtgaatttt
     3241 atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa gagaagggcg
     3301 aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg tgcccgaaac
     3361 aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt aacggcgatg
     3421 aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt ggcaaaatca
     3481 aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta agtccggatg
     3541 agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg gttactcaca
     3601 actcattgaa tctgcatgat attgcctgcc gggtaaggcg tcacgccgca tccggcatca
     3661 aacgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa aatgctcgcg
     3721 caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg cttcatgacg
     3781 cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc ctgccatgag
     3841 ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg taaacatcct
     3901 gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgag
//
LOCUS       DAEEBB010000249         3923 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEEBB010000249 DAEEBB010000000
VERSION     DAEEBB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.249"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..301
                     /locus_tag="KLF07_004158"
     CDS             <1..301
                     /locus_tag="KLF07_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9833990.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="KLF07_004159"
     CDS             390..2258
                     /locus_tag="KLF07_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBD9833991.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..3923
                     /locus_tag="KLF07_004160"
                     /pseudo
     CDS             2295..3923
                     /locus_tag="KLF07_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1232 a          721 c          769 g         1201 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtatg gtgtcggtga tggcggcgct ggccccggtg aggaacatat agaacgatta
     3721 acacgtatac gcaatattga tggcttacct catgttgatt tcagccgtgt tgataagttc
     3781 ttcacttacg ccgacgcctt tcgcgaaagc ttgcccatta tttcaggtga actttatttc
     3841 gaggctcatc aagggtgctt tacatcagag tcagcaacta aagctcataa tcgaaatatg
     3901 gaaaataaat tacatgatgc tga
//
LOCUS       DAEEBB010000250         3909 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEEBB010000250 DAEEBB010000000
VERSION     DAEEBB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.250"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            25..591
                     /locus_tag="KLF07_004161"
     CDS             25..591
                     /locus_tag="KLF07_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBD9833992.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            595..1170
                     /locus_tag="KLF07_004162"
     CDS             595..1170
                     /locus_tag="KLF07_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBD9833993.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1212..2264)
                     /locus_tag="KLF07_004163"
     CDS             complement(1212..2264)
                     /locus_tag="KLF07_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBD9833994.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2489..3409
                     /gene="lpxL"
                     /locus_tag="KLF07_004164"
     CDS             2489..3409
                     /gene="lpxL"
                     /locus_tag="KLF07_004164"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBD9833995.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3581..3778
                     /locus_tag="KLF07_004165"
     CDS             3581..3778
                     /locus_tag="KLF07_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBD9833996.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          988 c          951 g          999 t
ORIGIN      
        1 cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt
       61 atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt
      121 ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat
      181 aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata
      241 tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct
      301 ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca
      361 actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc
      421 gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc
      481 gtagtactgt ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac
      541 gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa
      601 aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc
      661 gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaatttccg catccagcac
      721 cttggctata gctggttata cggcaccttt aaagatttcg acggtacttt tacctttgac
      781 gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact
      841 aatcacgccg aacgcgataa acatcttcgc agtgcagatt tcctcaatac cgcaaaatat
      901 ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc
      961 ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt
     1021 cagggcgacg acccatgggg tggtaaacgt gcaggcttcg aggccgaagg caaaattaag
     1081 ctcaaggact tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt
     1141 atttcagtgg aaggcgtaca gcagaagtaa gcgtgaaatg ttgccggatg gcacgcatcc
     1201 ggcaatgata tttattctgt tggatcagga atgcccagtg ttgtattcag acgtccacga
     1261 gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct
     1321 ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg
     1381 catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg
     1441 tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc
     1501 atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc
     1561 gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta
     1621 tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt
     1681 tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta
     1741 tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc
     1801 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
     1861 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
     1921 tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
     1981 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
     2041 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
     2101 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
     2161 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcaac caacattttg
     2221 gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc
     2281 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
     2341 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
     2401 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
     2461 cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
     2521 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca
     2581 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
     2641 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
     2701 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggactgat
     2761 ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
     2821 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
     2881 ccattttctg acactggaac tgggcgcgcg gcagtttggt attcaggaac cggggattgg
     2941 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
     3001 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
     3061 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
     3121 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
     3181 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
     3241 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
     3301 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     3361 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc
     3421 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
     3481 agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt atcttttctt
     3541 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtggaaatga
     3601 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     3661 tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     3721 cggtcactcc gccctgaata tgtggtccgg tattgtcttc agtataatac cttcctaaat
     3781 acactacctg agagaagaga tgaaaagagc tatcagctac atgaggtttt caactgagaa
     3841 gcagataaaa ggggactcta tacgaagaca gtctaagctg gtcactgact ggttatcaaa
     3901 gaaccctga
//
LOCUS       DAEEBB010000251         3866 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEEBB010000251 DAEEBB010000000
VERSION     DAEEBB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.252"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            752..1468
                     /gene="nanC"
                     /locus_tag="KLF07_004166"
     CDS             752..1468
                     /gene="nanC"
                     /locus_tag="KLF07_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBD9833997.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1488..2594
                     /gene="nanM"
                     /locus_tag="KLF07_004167"
     CDS             1488..2594
                     /gene="nanM"
                     /locus_tag="KLF07_004167"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBD9833998.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2659..3639
                     /locus_tag="KLF07_004168"
     CDS             2659..3639
                     /locus_tag="KLF07_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBD9833999.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1163 a          801 c          877 g         1025 t
ORIGIN      
        1 gacttcagaa gcgaccagtt acaagccaca aatcaggaaa catatttcat ggtgagaatt
       61 atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag ttagcttgca
      121 aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt aaaagaaatc
      181 ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat cttcctctta
      241 gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg tatggcttgc
      301 gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa tgagacaggg
      361 catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
      421 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
      481 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
      541 agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg tggaaagaga
      601 attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc aaagacacta
      661 taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
      721 gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
      781 attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg acgtacgtgg
      841 tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg agggatggca
      901 aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg ataataaaaa
      961 ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta aacttgatga
     1021 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     1081 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     1141 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     1201 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     1261 aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga aatgggcgac
     1321 ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat acatagaata
     1381 tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     1441 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacta
     1501 taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     1561 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     1621 taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat aaaaaatgga
     1681 ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca tttattgatg
     1741 gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact caggtattta
     1801 atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg tcgctcgcgc
     1861 cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat gttactggcg
     1921 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     1981 caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa gattatttct
     2041 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     2101 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     2161 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     2221 gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc gtcgctggcg
     2281 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     2341 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     2401 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     2461 gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt ggcggtgaaa
     2521 ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat aataaagtca
     2581 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     2641 gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta tgttcttacc
     2701 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2761 gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca tcccggaggc
     2821 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2881 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2941 caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc agggattctt
     3001 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     3061 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctgtaccag
     3121 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa attcctcggc
     3181 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     3241 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     3301 aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg gaaagaaaat
     3361 ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     3421 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     3481 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     3541 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaga
     3601 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3661 gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa ggtaatatct
     3721 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3781 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3841 catccgcgcc ttttcgatta tggctc
//
LOCUS       DAEEBB010000252         3864 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEEBB010000252 DAEEBB010000000
VERSION     DAEEBB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.253"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="KLF07_004169"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="KLF07_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="KLF07_004170"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="KLF07_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBD9834000.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="KLF07_004171"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="KLF07_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBD9834001.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="KLF07_004172"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="KLF07_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBD9834002.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          845 c          959 g         1072 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAEEBB010000253         3858 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEEBB010000253 DAEEBB010000000
VERSION     DAEEBB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.254"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="KLF07_004173"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="KLF07_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBD9834003.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="KLF07_004174"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="KLF07_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBD9834004.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="KLF07_004175"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="KLF07_004175"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBD9834005.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="KLF07_004176"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="KLF07_004176"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBD9834006.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAEEBB010000254         3832 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEEBB010000254 DAEEBB010000000
VERSION     DAEEBB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.257"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..266)
                     /locus_tag="KLF07_004177"
     CDS             complement(<1..266)
                     /locus_tag="KLF07_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD9834007.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="KLF07_004178"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="KLF07_004178"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBD9834008.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="KLF07_004179"
     CDS             complement(726..998)
                     /locus_tag="KLF07_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBD9834009.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="KLF07_004180"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="KLF07_004180"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBD9834010.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="KLF07_004181"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="KLF07_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tg
//
LOCUS       DAEEBB010000255         3797 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEEBB010000255 DAEEBB010000000
VERSION     DAEEBB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.258"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="KLF07_004182"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="KLF07_004182"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBD9834011.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="KLF07_004183"
     CDS             1027..1485
                     /locus_tag="KLF07_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBD9834012.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="KLF07_004184"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="KLF07_004184"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBD9834013.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="KLF07_004185"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="KLF07_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBD9834014.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="KLF07_004186"
     CDS             2691..3368
                     /locus_tag="KLF07_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBD9834015.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="KLF07_004187"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="KLF07_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBD9834016.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          952 a          904 c          989 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgca ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAEEBB010000256         3735 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEEBB010000256 DAEEBB010000000
VERSION     DAEEBB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.259"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..182)
                     /locus_tag="KLF07_004188"
     CDS             complement(<1..182)
                     /locus_tag="KLF07_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HBD9834017.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="KLF07_004189"
     CDS             426..683
                     /locus_tag="KLF07_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBD9834018.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="KLF07_004190"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="KLF07_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBD9834019.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="KLF07_004191"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="KLF07_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBD9834020.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="KLF07_004192"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="KLF07_004192"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBD9834021.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3735)
                     /locus_tag="KLF07_004193"
     CDS             complement(3308..>3735)
                     /locus_tag="KLF07_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBD9834022.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          953 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaatt
//
LOCUS       DAEEBB010000257         3673 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEEBB010000257 DAEEBB010000000
VERSION     DAEEBB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.260"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(135..698)
                     /locus_tag="KLF07_004194"
     CDS             complement(135..698)
                     /locus_tag="KLF07_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD9834023.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(802..1020)
                     /locus_tag="KLF07_004195"
     CDS             complement(802..1020)
                     /locus_tag="KLF07_004195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834024.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1179..1565)
                     /locus_tag="KLF07_004196"
     CDS             complement(1179..1565)
                     /locus_tag="KLF07_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD9834025.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1555..1926)
                     /locus_tag="KLF07_004197"
     CDS             complement(1555..1926)
                     /locus_tag="KLF07_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD9834026.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1926..2183)
                     /locus_tag="KLF07_004198"
     CDS             complement(1926..2183)
                     /locus_tag="KLF07_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834027.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2180..3571)
                     /locus_tag="KLF07_004199"
     CDS             complement(2180..3571)
                     /locus_tag="KLF07_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD9834028.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3568..>3673)
                     /locus_tag="KLF07_004200"
     CDS             complement(3568..>3673)
                     /locus_tag="KLF07_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD9834029.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          862 a         1025 c          819 g          967 t
ORIGIN      
        1 cacgtttaac gtcctgtgcc gtttttcagg tataaaaaaa cccgctcatc ggcgggttta
       61 agctgtgtgg cgtagtaacc actcttaaca ggatattcaa ctttttacga tcgtaaagtt
      121 ttcggaaaaa atttttaaaa ccgcatcaac cgctccacca gttgctcttt acgggcaacg
      181 atccacccgt gttgctccag ataaaattta aaccgttcca gagtacatac catcgcatcg
      241 gcgggtacct tttccgtgaa ctcaacctga ccgtgtttat cgaagtggct cagtaatgcg
      301 catccatcat tttcgataga tgtgttttgt gccgctggtg gctgtttctg gttgaaataa
      361 cagtcttcca gtttttcgaa cacttcccac gcctgatcgg tttcgagcat tttggcgtga
      421 cgggctgcgc cacgttctgt ccagaggatg agggagcgaa cgttacgggc aattttcaca
      481 gaaactctta aagagttttc gttcttcaac tctcgtaacg catcaccttc tatcaggaaa
      541 aaatgtttac cttctataaa ccgttctttg tttcgattga aattgtttgt caaacgctgg
      601 cgctctgttc cgtacaaatc agccagcagc tctgttgtca tgacaggaag atggttatgc
      661 gtaagtgacg ggagtttttc aatagacgtg cgattcatag acatgtcctt tcgaatattt
      721 tgatttaccc cttttgagag ggtgaccggg cgctcaaaac cgtcgaaagt cggcgggcgt
      781 atttccattg ctggtattgt attagccgca cacccggtca taaaccaaga attctggaca
      841 caaaaaaacc acatggctat cgggtgtgga taccgctttc gaggtgtttt gagcaccgcg
      901 gggaaatgta aactgcaaac ctgaaactgt caacaccgtc aggttccaat catcgggtgg
      961 tgagacgcac agggttggaa ctaccgggaa accgaccggc gagcttttca gctcccccat
     1021 gcgccccacc ataattcaga tgtgcgcgtg catacgacaa taaaaaacac gctcgcggcg
     1081 tgtgtctgtc gcggtctcta tccggggttc caatcccgac gccagatttt gctggcgcgt
     1141 gaggaatata gccccggaca atgtgtctgg tcaagcacct acataattcg ttctacgtat
     1201 ctgtccatct ccagccggat atcaagcatc atcaacatgc catcaataac cccttccgct
     1261 ttctgcaggc gcttgccaat acaggtatcc gaacacccat gtttccgtgc cagactcata
     1321 aaagtcattc cacctacgta ataatccacc agcaaatcgt gcaaatcctg atttttcttg
     1381 ttcaaccggg ccatacagcc acaaattatc attgcatcat catcagaaca ctgagggcgt
     1441 gatttcactt tcgacggaat taacccttta aaaccagcag cgatcgacgc ccaggataca
     1501 tcttcatgag tgtttgcagc ccaagccccc caccgttcca taacctgctg aatatcacgc
     1561 gccatcgtta tcacctgtga tttcataaat cttcacgccc agtcgcccac caggaacgag
     1621 ctgaccgcgc acaatattga tttcatcaaa ctgctcgtcg tctatgagta gtccggcatg
     1681 cgtcagcgca tccagtggtg ctttcaggat attgtccagg tcacgacggc gcttatccgg
     1741 tggctctgca ataatcttta tcgccagcct tccggacagg tttaatttca gccgctgctg
     1801 gcgaacaatt agcgtcacat cacggcgata acgctcacca acttttgata caaaatatgt
     1861 gctgccacga cgtcgccagt aagtattcac cgtcggcgga taaggtaaaa taaactcatg
     1921 gcgcatcagc gcagcacctc ctgcaccatt ttttcaaact ttccaacttt ggtttccagc
     1981 tctgccacac aatcaacgag ctcatccacc gctttttgtg cgcgatgttt cgcctgcatc
     2041 agttccctga gcgctggcac catatcccga cgaatggcat cttttgttac acctgttttt
     2101 tccagttgtt ccgcctgtcg caacatttcc tgcgcgtgtt tacgcaattg ttcagaggta
     2161 aaagtcattg tctggttgtt caaaagaaac gctccatctt actgctgtcg gtacgtgtat
     2221 tgctgtatct gcgcggctgg ggctgccgca ttggggtgga aagaacctgt gcgctttcct
     2281 ggtccacagg cagaaaatgt ccgttataaa aacgccggta aatcgtcccc agagaaccgt
     2341 tacgttgttt cgtgatatta atttctgcta tgcctgtagc ctgtgtatcc gggttgtaca
     2401 cttcatccct gtaaagcatc agaatgatgt ctgcatccgc ctctatttct ccggaatttt
     2461 tcaggtctga gttcatggga cgtttattgg gtctggactc cacaccgcgg gagagctggc
     2521 tcagcgcaat caacggaaaa ccaccggatt ttgccaggcc tttaagcccc tttgagattt
     2581 cacccacggc aaggtcatga cgccccgtgg ttcgggtttt tatcagcccg agataatcaa
     2641 ccaccaccag cgccgtttcc ggatgtttaa tcagatggtg tttcgttgtt gcgcatatct
     2701 catcaatggt caggttcgcc tggtccacca tccagatatt gcgcccggtc atccgcccca
     2761 ccccttgtga gaaacgcgcc cagtcttcat cttcaaagtg agccacagat ttcaggcgtg
     2821 atactggcat ccctccagcc gcagacacca tacgttcacc aatctggatg ttcgccatct
     2881 ccatggtgaa cagaagcaca ccatgcccct gctcagtcac cttgtcgatg atgtccagcg
     2941 caagttcggt tttccccatc gaaggacggg cggcaatgaa taccaggtct ccgggctcca
     3001 taccgcctgt ttttgcgtcc agttcatcaa taccggtcat caacgtcctg gatttctcca
     3061 gcccctgatt ccggcattca acacgctcaa ccacttccgg aagcacatca tcaatatgta
     3121 ccggctgaat gacgcccttt cctgtcgaca gtgtgaccat catgttctgc gcatccttca
     3181 gggcatcttc agctgcttca caggtatgcg catcacgtaa tttctgcagc gcctcattca
     3241 gtgttttttc tgcatcgcgc agtgcggcat tgcgccgcaa cgctgcaaca tagtgctcca
     3301 gtgaagactt cacccaggtt ttacgcccgg tatcagtaat caccggggca agttccggca
     3361 tctcattaca caacagcacg gggtcaatca ctcctgaaac acgggcctgt ctgcagatgc
     3421 ctgtgtagat atcccgatac gctcgtacag aaaaaatgtc cgccggtagt gtgatcagaa
     3481 tatccatcac ttcatgatct gccccacgca gaaagaatgc gccaatgaca gcgccttcca
     3541 ggtcatcatt acgccagact ggtgttgtca tgcagccaca cctctgatac aagaacggta
     3601 gctgggccag ttgaacgaca accagttgcg ccccccgtct gtgatcctgt cggcaatgcg
     3661 ggggctgatg aac
//
LOCUS       DAEEBB010000258         3642 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEEBB010000258 DAEEBB010000000
VERSION     DAEEBB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.261"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1272)
                     /gene="mxiA"
                     /locus_tag="KLF07_004201"
                     /gene_synonym="virH"
     CDS             complement(<1..1272)
                     /gene="mxiA"
                     /locus_tag="KLF07_004201"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBD9834030.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSF"
     gene            complement(1285..2352)
                     /gene="mxiC"
                     /locus_tag="KLF07_004202"
     CDS             complement(1285..2352)
                     /gene="mxiC"
                     /locus_tag="KLF07_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBD9834031.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(2371..>3642)
                     /gene="mxiD"
                     /locus_tag="KLF07_004203"
     CDS             complement(2371..>3642)
                     /gene="mxiD"
                     /locus_tag="KLF07_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBD9834032.1"
                     /translation="NISDKTFYISGPPALVELVANTATLLDKQVSSIGTDKVNFGVIK
                     LKNTFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAQNDPMPPFNITQKVSE
                     DSNDFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDVAKRHIEL
                     SLWIIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALNQ
                     KKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSSR
                     GQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTHE
                     TNSNEIVSIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIKESSYYNTAEYKSLI
                     SEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
BASE COUNT         1212 a          738 c          519 g         1173 t
ORIGIN      
        1 aaaactatcg gcacgcacct catttattaa caaaacaata tcatcgacct taagctcatt
       61 gcttgttcta tataaaatag ttggaagcct gacaccataa tcaataaaga actgacttct
      121 tatcctttca ataagacctt ccatatcatt tgcatttatc ttattttcgg caaataataa
      181 aattaaagga acggtttctg aactaatagc atccagattt tctatcattg ccaaagatga
      241 gtcatggcta ttatcaatat caaatgtacc agtatagcca ctagaatcag actcagacag
      301 acttttttct ttttctacga cctttttata atagaataaa gccgtcaaag taactgctat
      361 caggaaaaaa acaaaaaacg gaaaacctgg caacatccct atcgccaagg caagagctga
      421 tgtaacaatg agaacaaaag gattcccaaa tatctgggac attatgttac gtcccatatt
      481 atcgctatca ccattaactc ttgtaacgat aaatcctgca cttatagaaa ttaacagagc
      541 aggaatctga gagaccaatc cgtcaccaat agttagtatg gtataagtag ataatgcacc
      601 agatagggac attccatgtt gactcatacc aacagaaata cctcctatta aattgacaaa
      661 aataataatg atgccagcaa ttgcatcacc tttaatgaac ttcattgctc catcaaatga
      721 accatacaat tgactctcac gttccagaat actgcgtctt tctttagctc ctgcggcatc
      781 aataattcca gcctttaaat ccgcatcaat gctcatttgt ttccccggca taccgtcaag
      841 agaaaagcga gcagctacct ccgcaaccct ttcagatcct tttgttataa caatgaactg
      901 cacaacagtt actattgaaa aaataacaaa acctacagcc agactatcac caatgacaaa
      961 ctggccaaat gtagtaataa ttttacccgc atccgcatca actaaaatta gtcgactagt
     1021 acttattgat aaagcgagac gaaataatgt cgttatcaac aaaaccgaag ggaatgtaga
     1081 aaaactcaat attctttcaa tataaaatga tcccataaaa acgagaatag caagtacaat
     1141 attaagccct attaaaaaat caacaaggta tgtaggtaat ggtatgatca acatagcaat
     1201 gatcataacc ataagcacta atataattaa ttcaggctta gtacttactt gcttaagaaa
     1261 agactggatc actttcatct cctgttatct agaaagctct ttcttgtatg cacttgtgac
     1321 aagctctcgt agaatattga tcgcaatttc tctttcactt tcacttttaa atatatcatg
     1381 aggactttca ttaacagaag atagatatgt ttgatacact acagaatcct catgactggc
     1441 tcgtgatact tctataacag aagtaagaat ttgctcactt tcacttggat attgaaaaat
     1501 atataataaa gttagcaata gctgctctgc acttaaactg ccatctttta gcaagcttga
     1561 ggattccagt ttcttcataa aaattacgtc tgaagttcta atcatcttaa ttgcaattaa
     1621 tttagataac acaaaaccaa actcataagc attacagctc ggtttctcag aatccatgtc
     1681 tacaatcagt gactgctcta caaaattcac aattgtatgt ctgtgattaa aaccaaaatt
     1741 accaagccat tctatatact gatctgttgt tgatatgttc cccatgataa aaccacgata
     1801 acatgcacgc aaaagctgtg gttcaagttt cattttgttg ccaaataatt tagcctgtat
     1861 agctgaattt attccagcat taacactttt ttcactacct gatattatct cattaataat
     1921 tttagttaat gattcgactt gttcagcagt aagatctttt tcattcaaaa gttcccttaa
     1981 tgctaagact tgatcacttg gatctttaaa atatctcttt agtctgtcta taaaatcccg
     2041 atctagagga aggttatctt ttaacgttct ctttaaatca aaaatcttgt gattaatttc
     2101 atcttcttcc ccatctaaaa tacgttcctg actatcacta tttgttcctt tcagtttctc
     2161 aaggtctctt ctgtttataa atgacgaaag agcggaagcc atctcatcag atgagtcaat
     2221 atacttgcta ttagccaagc cagaatcaag ctctgcatca gcagtctcat ctccatcatc
     2281 tgaatttgtc attgcattca acttatcaat gaatgcagag ctaaaaactc ctgtattttt
     2341 aacatcaagc atacatcacc tactttatag ttagtaattt aagtatgaaa ccaatgattt
     2401 ttcgtcttcc agcaaagtag tttcagatgg gattatttgt gtagtctttt gtatttctct
     2461 ttcagatatg aggcttttat attctgcagt attataataa cttgactcct ttatctcacg
     2521 gggttgaatc agaaaaaccc ttacaatatt actgatatta cttgttttat acttgaacac
     2581 attaccgatt acaggaatag aggagagaaa aggaatagat actatttcgt tagagttcgt
     2641 ttcatgcgta taaccaccta tcaacaaact tttgccttgt ggaactctcg ctattgtact
     2701 gattttggtc cttccaactt ctggaaggac agatgtattt tcattattat aattgtaatt
     2761 tgactgacta tttcctgttc catcctctat cgttaaagac atctcaattt gtccccgaga
     2821 tgaaaagcga ggtataacat ttatcaatgt tccatacgta acatgctcca acgaagaatt
     2881 gcgctcacca actaatgaca cataaaaagt cctgttatta tcaaatatag ccggaatatt
     2941 ctcttgtgtc aaaatcaccg gccttgaaac tacatttgct ttctttttct gatttaatgc
     3001 cataactgaa gcaataaact tattgccgtc caaagtgctt atcgatgctg atgagctcat
     3061 attaaatgac gcaccaaacg aatcaccaaa tgaagccgtt ccttgccaat taacaccaag
     3121 gttatttaat tcagatttat caatatcgat tatccataat gataattcta tatgtcgttt
     3181 ggctacatct aactgagtga taatatcgcg aataatctgt atttgctgat cattaccttt
     3241 gactaatata gaattggttt ctggatatgc aataagtgat acatcctcta atatagaact
     3301 attggtaact gaactaaatg aaaagtcatt actatcctca gatactttct gtgtaatatt
     3361 aaagggtggc atcggatcat tttgggcttg tctgttactc agagccttac cattatttaa
     3421 taaacgttct acaacagtag ccacccctgg gatgacaata tcctcccctc tcatattata
     3481 agttctgtca ctaacaaatg tattttttaa ttttataacc ccaaaattaa ctttatctgt
     3541 cccaatagaa gaaacctgct tatcaagcaa agttgctgta ttagctacca gttcaaccaa
     3601 tgccggagga ccagatatat agaatgtttt atcactaata tt
//
LOCUS       DAEEBB010000259         3584 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEEBB010000259 DAEEBB010000000
VERSION     DAEEBB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.262"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            63..387
                     /locus_tag="KLF07_004204"
                     /pseudo
     CDS             63..387
                     /locus_tag="KLF07_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(417..3329)
                     /locus_tag="KLF07_004205"
     CDS             complement(417..3329)
                     /locus_tag="KLF07_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834033.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3332..3448
                     /locus_tag="KLF07_004206"
     CDS             3332..3448
                     /locus_tag="KLF07_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD9834034.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
BASE COUNT          898 a          963 c          882 g          841 t
ORIGIN      
        1 ggcaaaggcc atgaaagccc tgatttccgg aagcgagcga acagtccaca aactgttgaa
       61 cgtggtgtca gcggcggaca gggataagcc ctcgtcaaca gttccgtcag cactgcgaca
      121 gcgttcttta cccggtcaaa acagcgttgc ggtgcaccac gcctgacgga tgaactgcgt
      181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
      241 agggcaaagg cctcccggaa gttcagcccg gtcagttacc gcgcacatgg cctgcccgtg
      301 tcagaaaatc tgctggagca ggatttttac gccagtggtc cgaaccagaa gtgggcggga
      361 gacatcacgt acttacgtac agatgaagtt cggttacatc ctgtcagcac agaacctcac
      421 gctttttgag cggcacgacg gatccccccc acccagtatc cgacagactt ttcactgcgt
      481 tcgtaaaaac ccttcttccc tcccagcata tccactaatt ccggtgacat atcctgcaga
      541 taagtctgca aatcatgccc cagatcttcc ggtgactgca actcataaac cgcgcgccac
      601 acatcaccct gccttatttt aagccccggg ttaagaatat actgtccgtg gttcagcctc
      661 agaaaaagct tttttccata aggatttttg ctgttaactt catgttgaga gagctggctg
      721 ctgatataac tccttcgttt gcggcgttcc ggcagcaacg cttcaggaag atgaaccaca
      781 cactctgcca gtcgtgccgc actgaaggct tccgaaaaaa acagagcact tcccagatgc
      841 gtgtggaaca gcgcctgcat caggatcacc agaaacattg ccatctgccg gttgtccagt
      901 ttgatgaggc gtccttccac ctgcagtgac agactttcag gtttcagcag ggtatacaac
      961 tgcgcacttt tctgttgcgc ataacggggc gtactgaccg ccgctgacag aacttgatga
     1021 taagccgcca gcccgtcatt accggtcagc aagggatctg caccacgctc cagcagtaac
     1081 tgaaccagtg caatattacc tgcccgggct gctgacatca gcggagtaag attaaacggc
     1141 gaacggtgat caacaccata acgttcaata tcccgcaaca cggcaaccgg gttattcatt
     1201 gagtatggcc ggaaatactt cagctccagt aactgcagag tcttcgttct gggctgcctt
     1261 gtgggcttaa aatcttcccg gtacagtgca ttatacagcg ggaacaggga ataaatcaga
     1321 ctgtactcca gcgcctgcag ttgtatggtt ttatcggctg tacctgcgag gatatgctgt
     1381 ttccactgtc gtgcatcctc tgcggtgata atctgccatg gcatctcaga tgtctgcagg
     1441 atccggctac ggatggtgtc cgcctgctcc tgcttcccct gtttttcaag cagatgtgcg
     1501 gttttctgcc actcatcacg gctggaaatc tcttcctgca gattcagagg ctcttcctga
     1561 tgcgtgagcg caagcagact ccacagagga tgctccacct gctgttcaac aagatacaca
     1621 ttgtgtgtag cacgggtgag cgcaacatac agcgcgttgg taaaaaattt atagatttcc
     1681 gctgaacgat cctgcctgtc ccgcggacgg gaataacggg cctcgccctc aagatcagcc
     1741 ggtgtaagac cctcgcaaat atcatcaaat gcctgccggg cagcgcttac tatattgtaa
     1801 aggatcaccg tttcgtattc cagcccctta acctcctgaa ccgaaaagac cagcggcgta
     1861 ctgaaccagc atcgcgcctg tgctttctgt tccggatgca tcacaatgac cgctacacga
     1921 ttcgaaagtg aggttttggc attcagttcc tgctttgtct cttccctgtc gtccagcaac
     1981 cggatggtcc cttctgcctg accgcagctt ctgacaaaat ggtgactttc ccgatcaata
     2041 gcactaaagc ggacctgttt gagtctcagc aaccggttag ccagtgcagt gacgtggtgc
     2101 ccgttacggt aattcgcctg caggatatta acggttgtgt cgtttccctg ttgctgccgg
     2161 aaaaagagac ttttgagcga actccatgag aaaaagctcg gatgaactat ctgattggca
     2221 tcaccacaga gtaaaaactg accgggatga cgtagcgttt tcagtaccag ttgcagttga
     2281 acaggcgtca tatcctgaac ttcatcaaca aaaatgacat cataacgggg agttgccaac
     2341 gaaagatact gatgactgag cagattactg tcatactgat gcgactgttt cagccatgcg
     2401 atataacgtt caaacaggac atagaccgtc gggcggtcct ccatgccata gagcgactgc
     2461 cgtatcccca gtgacagata cgcctcccgg gacagcggcc cattcccgga ggcaaccgca
     2521 ccgatcacgc ccctgaactc ctcatacagg gtatgggcag cccccagtgc ggcgatagcc
     2581 ctgttgcggg gcagccagtc gctgaacgca ctgaaagtca cctccctgcc tttcggaatg
     2641 cgcagagttt ccagaaattc cgtcagcgaa agaaagtcaa tattctgtac ctcactccct
     2701 tcccctgaag cgtcatagag agtacgcgct ttttccacca gaaatgagga aaggctgaga
     2761 tacagtatat caccagcagc ctgcttcatt ttttccagta ccagcgccgt cttaccactt
     2821 cccgccgagc caacaatcac cagcggtagc gggtgttcgt acagtgcctg ctgatcatca
     2881 tcaaaggaca gcattttatc caggcggtgg aaacgcccat gagacggatt gatatacgtc
     2941 agcgcttcag tcgcgatatc aacaggatcc ggcactggtt gttgttgtaa gcgcccttca
     3001 tcaatcacca cgtttctgcg aagaaaacgt gaagtgttat aggcatgatt gcgaatatat
     3061 tccagaacaa ggcagatagt ttcaccgtgg tagcgataca gcgaaaacag taatcggtcc
     3121 cggatactga gccgggcgcg gtaaagattg gtgtcaattt tacgaacatc agcctgaaca
     3181 aagttatcgg tggcaatagc ctgtgcaaat tttttataac ccgggatcgt gtcaggattc
     3241 agagtatgat aaagacagat tttcatggct gccggtttcc gtcgtgcaaa aaacatgctg
     3301 attttacgaa ggaatgcctt actgaacaac cttgcccgga ataccccccg aaatcagatg
     3361 gaacaatcag aaaaatgctc tgcagttatg gaataaagaa ggtggggtgg tacgccggga
     3421 gggcgatccg ctggaaatgg cgcagtagct gcaggcctgt catgatgccg gaatagaagt
     3481 tcgtgtacag gttaatgaaa gcgtcacccc gtaaataaac tctgcgctga aattcctttt
     3541 tttcattgga gcacctgtgt tgttctgagg tgaacatatc acct
//
LOCUS       DAEEBB010000260         3559 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEEBB010000260 DAEEBB010000000
VERSION     DAEEBB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.264"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            129..1376
                     /locus_tag="KLF07_004207"
     CDS             129..1376
                     /locus_tag="KLF07_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBD9834035.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="KLF07_004208"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="KLF07_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBD9834036.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="KLF07_004209"
     CDS             complement(2228..2572)
                     /locus_tag="KLF07_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834037.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="KLF07_004210"
     CDS             2814..3416
                     /locus_tag="KLF07_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834038.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAEEBB010000261         3556 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEEBB010000261 DAEEBB010000000
VERSION     DAEEBB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.265"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..350
                     /locus_tag="KLF07_004211"
     CDS             <1..350
                     /locus_tag="KLF07_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBD9834039.1"
                     /translation="WYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIVE
                     LRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLN
                     TTGADQQSPEHRS"
     gene            590..850
                     /gene="repA"
                     /locus_tag="KLF07_004212"
     CDS             590..850
                     /gene="repA"
                     /locus_tag="KLF07_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBD9834040.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1074..1148
                     /gene="tap"
                     /locus_tag="KLF07_004213"
     CDS             1074..1148
                     /gene="tap"
                     /locus_tag="KLF07_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBD9834041.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1141..1998
                     /gene="repA"
                     /locus_tag="KLF07_004214"
     CDS             1141..1998
                     /gene="repA"
                     /locus_tag="KLF07_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBD9834042.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2698..2889
                     /locus_tag="KLF07_004215"
     CDS             2698..2889
                     /locus_tag="KLF07_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834043.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            2962..3213
                     /locus_tag="KLF07_004216"
     CDS             2962..3213
                     /locus_tag="KLF07_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBD9834044.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            3210..3497
                     /locus_tag="KLF07_004217"
     CDS             3210..3497
                     /locus_tag="KLF07_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD9834045.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          940 a          872 c          908 g          836 t
ORIGIN      
        1 ggtggtatct gtgtccgcag gtacgggtcg cggatatcgt ccagctgaac gggaatatcc
       61 ggccacgatc acgccagtgg tggcagttat tcaggatggt gtctcagtgg catgttgatg
      121 tggttatcgt tgagctgcgt tcgttcagta ttgttgcagc agtagagctg gatgatgcca
      181 gccatttacg accggaacgc agacgccggg atattcttct ggaagaggtt ctgaggcagg
      241 ctggtattcc gttgctcaga agccacgatg ccagaaaact gctgcagatg accggagaat
      301 ggctgaatac aacaggggct gatcagcagt caccggaaca tcgtagctga cgccttcgcg
      361 ttgcttagtt gtccaacccc ggaaacggga aaaagcaagt tttcctcgct cccggcgttt
      421 ccataactga aaaccatact atttcacagt ttaaatcaca ttaaacgaca gtaatccccg
      481 ttgatttgtg cgccaacaca gatcttcgcc acaattctca agtcgctgat ttcaaaaaac
      541 tgtagtatcc tctgcgaaac gatccctgtt tgagtattga ggaggcgaga tgtcgcagac
      601 agaaaatgca gtgacttcct cattgagtca aaagcggttt gtgcgcagag gtaagcctat
      661 gactgactct gagaaacaaa tggccgcagt tgcaagaaaa cgtcttacac acaaagagat
      721 aaaagttttt gtcaaaaatc ctctgaaaga tctcatggtt gagtactgcg agagagaggg
      781 gataacacag gctcagttcg ttgagaaaat catcaaagat gaactgcaga gactggatat
      841 actaaagtaa agactttact ttgtggcgta gcatgctaga ttactgatcg tttaaggaat
      901 tttatggctg gccacgccat aaggtggcag ggaactggtt ctgatgtgga tgtacaggag
      961 ccagaaaagc aaaaaccccg ataatcttct ttaactttgg cgagtcagaa agattaccgg
     1021 ggcccactta aaccgtatag ccaacaatca agctatgcgg ggagtatagt tatatgcccg
     1081 gaaaagttca agacttcttt ctgtgctcgc tccttctgcg cattgtaagt gcaggatggt
     1141 gtgactgatc ttcaacaaac ttattaccgc caggtaaaga acccgaatcc ggtgttcaca
     1201 ccccgtgaag gtgcccggac gcttccgttc tgcgggaaac tgatggaaaa ggcggtgggc
     1261 ttcacctccc gttttgattt cgccattcat gtggcacatg cccgttcgct tggtctgcgt
     1321 cggcgcatgc caccggtact acgtcgccgg gccattgatg cgctgctgca ggggctgtgt
     1381 ttccactatg acccgctggc caaccgcgtt cagtgctcca tcaccacgct ggccattgag
     1441 tgcggactgg cgacggagtc tgatgccgga acactttcca tcacccgtgc cacgcgtgcc
     1501 ctgacgttcc tggcagagct ggggctgatt acctaccaga cggaatatga cccgcttatc
     1561 ggctgcaaca ttccgaccga catcacgttc acaccggcgc tgtttgccgc cctcgatgta
     1621 tcagaggagg cggtggcgtc cgcgcgccgc agtcgtgtgg aatgggaaaa cagacagcgc
     1681 aagaagcagg gactggatac cctgggtatg gatgaactga tggcgaaagc ctggcgtttt
     1741 gtgcgtgagc gtttccgcag ttaccagaca gagcttaagt cccgtggaat gaagcgtgcc
     1801 cgtgcgcgtc gtgatgcgga caggcagcgt caggatattg tcaccctggt gaaacggcaa
     1861 ctgacgcgtg aaatctcgga agggcgcttc actgccagtc gtgaggcggt aaaacgtgaa
     1921 gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta accgtaatta cagccggctg
     1981 gccacagctt ccccctgaaa gtgacctcct cagaataatc cggcacgcgc cggaggcatt
     2041 cgcacgcctg aagcccgcca gtgcacaaaa aatcagcgct acatacaaaa aacaacctca
     2101 tcatccacct tctggtgcat ccggtccccc ctgtttttga tacaaaacac gcctcacaga
     2161 cggggaattt tgcttatcca cattaaactg caagggactt ccccataagg ttacaaccgt
     2221 tcatgtcata aagcgccagc cgttagcttt acagggtgca atgtatcttt taaacagctg
     2281 tttatatctc ctttaaacta cttaattaca tccatttaaa aagaaaacct gttcactgcc
     2341 tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc gaccgaagcc
     2401 gatttagtta caacatgcaa acataaaccc ctaaaaaacc caagtccgac gcagaactga
     2461 aaccacaacg cccctcctca taactgaaaa gcgccgctgc ccccgcccga agggcgggaa
     2521 cagagtcgct tttaattatg aatgttgtaa ctacatcgtc atcgctgtca gtcttctggc
     2581 tggacgtatc gagtacacgc tcgtaagcgg ccctgacggc ccgctaacgc ggagatacgc
     2641 cccgacttcg ggtaaaccct cgtcgggacc actccgaccg cgcacagaag ctctgtcatg
     2701 gctgaaaacg ggtatggctt agcagggctg gggatgggta aggtgaaatc tgtcaatcag
     2761 taccggctaa cgccgggctt cggcggtttt actccggtat cacatgtaac cgcagcgtgc
     2821 cgccttacat gccgctggcg cggcatcaga ataatacaag cagcatttaa tgcatttgca
     2881 aaagtgtgag taataaagac ttgttaagtg gtcgtttcag ggatatcgtt aagacttgtt
     2941 aaacagtctc aaaggtgcgt tatgcctaac atcattctgt ctgagaccag tgcaagtgtc
     3001 agtgaactga aaaaaaatcc tatggctaca gtcagtgccg gagatggtta ccctgtcgcc
     3061 attcttaacc ggaatcagcc ggcattctac tgtgtgcctg ctgaactgta tgaaaggatg
     3121 cttgatgctc tggatgacca ggaactggtg aaactggtta ctgaacgcag caatcagcca
     3181 ctgcatgatg tggacctgga cagttattta tgacttatac ggtaaaattc agggatgatg
     3241 cgctcaagga atggctgaaa ctggataaga ccattcaaca acagtttgtg aaaaaactaa
     3301 aaaaatgcag cgaaaatcct catataccgt cggcaaaact aagagggcta aaggactgct
     3361 acaaaattaa actacgtgca tcgggatttc gtctggttta tcaggtgatt gacgatatgt
     3421 taattattgc ggttgttgcg gtaggaaaac gagaacgtag taacgtttat aatctcgcca
     3481 gtgagagaat gagatagtaa catataaata acctttcccg cagataactc agaattgtta
     3541 ttttctctgt ggactc
//
LOCUS       DAEEBB010000262         3546 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEEBB010000262 DAEEBB010000000
VERSION     DAEEBB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.266"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..143)
                     /locus_tag="KLF07_004218"
     CDS             complement(<1..143)
                     /locus_tag="KLF07_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834046.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="KLF07_004219"
     CDS             complement(189..530)
                     /locus_tag="KLF07_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834047.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="KLF07_004220"
     CDS             complement(699..890)
                     /locus_tag="KLF07_004220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834048.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="KLF07_004221"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="KLF07_004221"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBD9834049.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="KLF07_004222"
     CDS             complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="KLF07_004222"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBD9834050.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgc
//
LOCUS       DAEEBB010000263         3451 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEEBB010000263 DAEEBB010000000
VERSION     DAEEBB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.267"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            6..326
                     /locus_tag="KLF07_004223"
     CDS             6..326
                     /locus_tag="KLF07_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HBD9834051.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="KLF07_004224"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="KLF07_004224"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBD9834052.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="KLF07_004225"
     CDS             2213..2929
                     /locus_tag="KLF07_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD9834053.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          954 a          760 c          752 g          985 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     3421 taacagtccg ccgttctacc gactgaacta c
//
LOCUS       DAEEBB010000264         3436 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEEBB010000264 DAEEBB010000000
VERSION     DAEEBB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.268"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..523
                     /gene="fliD"
                     /locus_tag="KLF07_004226"
     CDS             <1..523
                     /gene="fliD"
                     /locus_tag="KLF07_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBD9834054.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
     gene            538..948
                     /gene="fliS"
                     /locus_tag="KLF07_004227"
     CDS             538..948
                     /gene="fliS"
                     /locus_tag="KLF07_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBD9834055.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            948..1313
                     /gene="fliT"
                     /locus_tag="KLF07_004228"
     CDS             948..1313
                     /gene="fliT"
                     /locus_tag="KLF07_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBD9834056.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            1391..2878
                     /gene="amyA"
                     /locus_tag="KLF07_004229"
     CDS             1391..2878
                     /gene="amyA"
                     /locus_tag="KLF07_004229"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD9834057.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(2912..3325)
                     /locus_tag="KLF07_004230"
     CDS             complement(2912..3325)
                     /locus_tag="KLF07_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD9834058.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT          922 a          820 c          885 g          809 t
ORIGIN      
        1 atataaagaa gtagacccag gtgctgaagc acaagataaa aataatggtg cacttctcgg
       61 agacagcgtt gtacgtacaa ttcaaagcgg catccgcgca caatttgcaa acggtgccag
      121 cgacagtgcc ttcaaaacat taaacgagat cgggattaag caggacggca cgacgggtaa
      181 gctgaaaatt gatgatgata aactcaaaaa agtcctgaat gaaaatacag catccgtacg
      241 tgaattgctg gtaggcgatg gtaaagaaac aggaattacc accaaaattg ccaccgaggt
      301 aaaaggttac cttgccgatg atggcattat tgatagcgca caggacagta ttaacgccac
      361 actgaaaaaa ctgaccaaac agtatctatc cgtcagtgcc agcattgatg acaccgtggc
      421 gcgttacact gcacagttta cgcaattaga taccatgatg agcaaactga ataacaccag
      481 tacctatctc agccaacaat ttactgcaat gagtaattcc tgatcacgga ggtcagcatg
      541 tataccgcga gcggctcaaa agcctatgca caaattggcg tcgaaagcgc cgtaatgagc
      601 gccagccagc aacagctggt caccatgcta tttgatggag tgctgagcgc actggttaga
      661 gcgcgcctgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg
      721 atcaacatca ttgagaacgg gctgcgggtg agtcttgatg aagagagcaa agacgaacta
      781 acccaaaact tgattgctct ttatagctat atggtcaggc gcttgctgca agccaattta
      841 cgcaacgatg tctccgcagt cgaagaagtg gaagcattaa tgcgcaatat tgccgatgcc
      901 tggaaagagt ctttactctc cccttctttg attcaggacc cagtctgatg aaccatgcac
      961 cgcatttgta tttcgcctgg caacaactcg tcgataaaag ccagctcatg ttacgcctgg
     1021 caacggaaga acaatgggac gaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc
     1081 aggagattgc acatctgact gaagaggttg ccccgtccac cacgatgcag gagcagctcc
     1141 gcccgatgct gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc
     1201 gtatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatca gtgttaagtg
     1261 cctatggaga tcagggcggc tttgtgctgg ctccgcagga taacctcttt tgaatcttaa
     1321 cgagccgatg gctcgcgaat aatccgatta cggctacgct tctaatgttc cccttgaatg
     1381 gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg
     1441 cggtaagctc tggcctgaac tggccgagcg cgccgacggt tttaatgata ttggtatcaa
     1501 tatggtctgg ttgccgcccg cctataaagg cgcatcgggc gggtattcgg tcggctacga
     1561 ctcctatgat ttatttgatt taggtgagtt tgatcagaaa ggcagcatcc ctactaaata
     1621 tggcgataaa gcacaactgc tggccgccat tgacgctctg aaacgtaatg acattgcggt
     1681 gctactggac gtggtagtca accacaaaat gggggcggat gaaaaagaag ctattcgcgt
     1741 gcagcgtgta aatgctgatg accgtacgca aattgatgaa gaaatcattg agtgcgaagg
     1801 ctggacgcgt tacaccttcc ccgcccgtgc cgggcaatac tcgcagttta tctgggactt
     1861 caaatgtttt agcggtatcg accatatcga aaatcctgat gaagatggca tatttaaaat
     1921 tgttaacgac tacaccggcg aaggctggaa cgatcaggtt gatgatgaac tcggtaattt
     1981 cgattatctg atgggtgaga atatcgattt ccgcaatcat gccgtgacgg aagagattaa
     2041 atactgggcg cgctgggtga tggaacaaac acaatgcgac ggttttcgtc ttgatgcggt
     2101 taaacatatt ccggcgtggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc
     2161 aaagccgctg tttattgtgg cagagtactg gtcgcatgaa gtcgataaac tgcaaacgta
     2221 tattgatcag gtggaaggca aaaccatgct gttcgatgcg ccgctgcaga tgaaattcca
     2281 tgaagcatcg cgcatgggac gcgactacga catgacacag attttcacgg gtacattagt
     2341 ggaagccgat cctttccacg ccgtaaccct cgtagccaat cacgacaccc aaccgttgca
     2401 agccctcgaa gcgccggtcg aaccgtggtt taaaccgctg gcgtatgctc taattttgtt
     2461 gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcgc attacgaaga
     2521 tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga
     2581 gttaattctc gcccgtcagc gtttcgccca cggtgtacag acgttatttt tcgaccatcc
     2641 gaactgcatt gcctttagcc gcagtggcac tgacgaatat cccggctgcg tggtggtcat
     2701 gtcgaacggg gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac
     2761 ctggcgtgat tttctgggga atcggcaaga gagcgtagtg accgacgaaa acggcgaagc
     2821 gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat
     2881 tcatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt
     2941 tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat
     3001 cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt
     3061 tagtgagatc atcagacatc aatgtcaact tgcccggcac ggcaatcacc cgctgccact
     3121 gacggcaatc cagggtatcg ccctctttag tgacaatcag gctgcctatc tcttccggac
     3181 tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg
     3241 gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg
     3301 cacctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg
     3361 cattaaagcc ctgatgtcgc cagcctttcc aggttcagag agaaatttga tctaactatt
     3421 atttttgtga attatt
//
LOCUS       DAEEBB010000265         3377 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEEBB010000265 DAEEBB010000000
VERSION     DAEEBB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.269"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1694)
                     /gene="rsxC"
                     /locus_tag="KLF07_004231"
     CDS             complement(<1..1694)
                     /gene="rsxC"
                     /locus_tag="KLF07_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBD9834059.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
     gene            complement(1687..2265)
                     /gene="rsxB"
                     /locus_tag="KLF07_004232"
     CDS             complement(1687..2265)
                     /gene="rsxB"
                     /locus_tag="KLF07_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBD9834060.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2265..2846)
                     /gene="rsxA"
                     /locus_tag="KLF07_004233"
     CDS             complement(2265..2846)
                     /gene="rsxA"
                     /locus_tag="KLF07_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBD9834061.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            complement(2923..3363)
                     /locus_tag="KLF07_004234"
     CDS             complement(2923..3363)
                     /locus_tag="KLF07_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBD9834062.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
BASE COUNT          864 a          917 c          864 g          732 t
ORIGIN      
        1 cgggcgatag ctgcttcaac ggcagtttta cgtggatcag caacggttgc tgcgtcgtta
       61 gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc ccgtgctgca
      121 ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg tgtagcctgg
      181 gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc tttggctgca
      241 ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg ctccagacga
      301 gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc ctgacgaata
      361 gccgcaattt cagctttttc ctgacggaaa tattgcacca ggggaatatt gctcgggcaa
      421 acccacgcgc aagccccaca ttcaatgcaa tcagcaatgt tatgcgtggt agctttatcg
      481 tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccgc agggcaggcg
      541 tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc aagttcattg
      601 gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag ccatggcaag
      661 gtaaagccca ttagcgggcc acccataatc accatttgat cggcagaggg gcagaatccg
      721 gcatcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt gcccgggcga
      781 gcgattgctt cgccagtcag ggttacaaca cgctcggtaa tcggctcgcc atcaataacg
      841 gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat atcggatgaa
      901 cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt agcaccgcca
      961 gaaggatatt tggttggaat cacccgcaga gaaatatcgt tagagtccgc cagcaccgcg
     1021 cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat ttcgcgtggc
     1081 tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca atcctgcatc
     1141 aaacggtcat cggcggtaat gtacggctcg cactcagccg cgttgataat caacgtttca
     1201 atcttatctc cgccaccctg caatttaacg cctgtcggga atcctgcacc gcccagcccg
     1261 gcaacaccaa actgatgtat gcgctcgatt aactcttcgc gactgcgagt gcgataatcg
     1321 gcccagccgt cgcgcgggat ccagcagtct tcaccatcgg catcaataat cacgcttaat
     1381 tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac ggtacccgag
     1441 gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg gccgcgcaat
     1501 actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg tttcagtgga
     1561 ataacaaaac gctgcgccag tggtacctgg cgcaggggtg taccgttgga ctgggttttc
     1621 atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct gaatgcagag
     1681 aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt tcagatccca
     1741 tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg tcgggcacgg
     1801 atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca tggcacgggt
     1861 agcgccaacg atggcgtcta ccggacaagc ctgaatacat ttagtgcagc caatacagtt
     1921 attttcatca ataaccgcca ccatccgcgc aggcgttagc tcttgcgctt cgccatccag
     1981 cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt cgccacctgg
     2041 ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg ggcgacagcc
     2101 gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt tctcaacgac
     2161 cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc caaacgccag
     2221 gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa cttcaccaaa
     2281 ccattaaagc ccataaaggc cagagacata agacctgcgg taattaacgc aatggcatta
     2341 ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc ggcgaagagc
     2401 accatcacca gcgagaaacc gacagcggcg gaaaaaccgt acagcgccga ctgcaagaaa
     2461 ttgtgcccga gattgatatt cagcaacgcc acgccgagca ctgcacagtt ggtggtgata
     2521 agcggcaaaa aaatccccag cagacggtaa agcaccgggc tggttttgcg caccaccatc
     2581 tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg caggtaaatc
     2641 agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga cgccagcgtc
     2701 atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagtttttt ggaaaccccc
     2761 ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag tacagttccg
     2821 acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc gcctattatc
     2881 cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat tcacaaacgt
     2941 cgcttttacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg ccaacaaagt
     3001 aaacagcaac tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga atgcttttac
     3061 tgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac aacgccgacg
     3121 tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag cggtaataaa
     3181 agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt gaaatgtttg
     3241 cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca acagagcgac
     3301 taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg ttgtggtggt
     3361 cataggttct ccagaat
//
LOCUS       DAEEBB010000266         3285 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEEBB010000266 DAEEBB010000000
VERSION     DAEEBB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.270"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..229)
                     /locus_tag="KLF07_004235"
     CDS             complement(<1..229)
                     /locus_tag="KLF07_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834063.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="KLF07_004236"
     CDS             403..1116
                     /locus_tag="KLF07_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD9834064.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="KLF07_004237"
     CDS             1148..1906
                     /locus_tag="KLF07_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834065.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="KLF07_004238"
     CDS             1952..>3285
                     /locus_tag="KLF07_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBD9834066.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAEEBB010000267         3221 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEEBB010000267 DAEEBB010000000
VERSION     DAEEBB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.271"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..262)
                     /locus_tag="KLF07_004239"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="KLF07_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(377..1015)
                     /gene="ospI"
                     /locus_tag="KLF07_004240"
     CDS             complement(377..1015)
                     /gene="ospI"
                     /locus_tag="KLF07_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBD9834067.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1174..1644)
                     /locus_tag="KLF07_004241"
                     /pseudo
     CDS             complement(1174..1644)
                     /locus_tag="KLF07_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1980..2165
                     /locus_tag="KLF07_004242"
                     /pseudo
     CDS             1980..2165
                     /locus_tag="KLF07_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2265..2474)
                     /locus_tag="KLF07_004243"
                     /pseudo
     CDS             complement(2265..2474)
                     /locus_tag="KLF07_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2524..2703)
                     /locus_tag="KLF07_004244"
                     /pseudo
     CDS             complement(2524..2703)
                     /locus_tag="KLF07_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          908 a          805 c          657 g          851 t
ORIGIN      
        1 gcaagcgctg taactctttt atttgtttta tagcggcagc cagttctgag gcaggaacga
       61 catcctctcc ggcagcaaca gcagtgagac ttccttccag atattatttt ttccacttaa
      121 gcaacaggct gggctgaata ccatgcaaac gcgccacatg ggacaccgtc atgccaggct
      181 ccattgtctg ctgaataatg gcaatttttt cctgaggagt tttacgttta cgaagctcca
      241 gccccagcaa agttccagtc atctcaaaat tagaactagt gttagacata tatccaagcc
      301 tatctcttat tttaagatac atccactgtc tggcgtttca aggggccggc tcaaatctat
      361 gtacaggctc tcagcatcag caaagcctct tactcttcca actactactg ttagcatagt
      421 gatcaaattc ctgatcagat acatgaatta cctgaaccga actattttca tctacaaatg
      481 catctggcga tttttctaca ggatcataaa gattaaatcc ctttcctgtt tgaacatcca
      541 aaaacataat tttttcacca tcgttaaatc cagctatcca atgagtggcc tgttcaatag
      601 aaatcattac tacacttcct tttgaagtgt tttgtaaaat attttctgca atacgctcac
      661 gaaatttact ataagtttca ttaggatgtg acactatgcc ttcactatca atttgctcta
      721 aatgcctatg aaaaaatccg gtcatatact ctgatatttc attaggtacg ggagcctctc
      781 ttactccata ccctaacata gccatacaag catgaagcgc acaccctgaa cagttaccat
      841 ccccaccaga cggatttatc attttctgta aagtcgctgc caattcagaa tctttatcca
      901 tatcaccaag acttttagag tttaaagatt ccagcaattt tttaacattt acatttccct
      961 cctctgtatg tgcctcatat ccagcagttc cctgtaacga caccccatta atcatattgt
     1021 atcctgacag tatatatact cactatattt tcttcataac aaatatccct ctgatatgac
     1081 aacaaaaaac ggagccggat tcctgttttt gtgaactgca gctattcgag tctaaaaagt
     1141 gatccactta ccaccaacgg cctaatatat acagaacgga gtaaacaggg ctaacagttc
     1201 ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt
     1261 gttgtacgca taccagagcc agtgttgttg cagccttgct gccaacgaag cgccactgct
     1321 catcaagttc acagataagg gcaacatcag cgtgagcgac tggcgcagat gttatccgcc
     1381 ggggcgggag ctttttaatg tccgtatgac ggtattgatg ccgattttca gtgtcctggc
     1441 ggtatcacgg ccccctgcgc cgttatgtgc catttcaacg atctgctcct taacgccggg
     1501 ttttctggct tcataagagt aagtgagctg gaaaacacgt ttacatgagc gacaacggaa
     1561 acgctcatgc cgggaacaac tacggccatg tcgataaact tcatcggaat gacagcgcgg
     1621 acaatgaaca gtaacggtag ccatggaaaa ccttaaatac cagtagatta ccagaaactt
     1681 ctgtcgattg aagacatcac ccgaagtttg aattcattcg aatacttttt acgaggttct
     1741 tttcgccagt cctgtaattc catacttagg tgttcgtcta tgtcagatgg gcgtctaagt
     1801 taccaattct tgtctaatgg ctacatacgg tggtcggttt acgcttaccg caggacaggt
     1861 ggtaactgaa aaatagtttg ctgaggcgac tggaaacaga agggcaagat cgattccgac
     1921 agcagtgatg gtcagctacc tcatcgataa ctttgcaaca gtgcccttct tgaagcatca
     1981 tgcttcatga agaaaaactg gcacgtcatc aacgtaaaca ggcgatgtat acccgaatgg
     2041 tagccttccc ggcggtgaaa atgttcgaag agtatgactt cacgttcgcc accggagcac
     2101 cgcagaagca gctccagtcg ttacgctcac tcagcttcat agagcgcaat gaaaacatag
     2161 tgttacaggg gacatctgac atcacaaatc cccgggtggg gatttgtgta taagagacag
     2221 cccatggagg aaggggatat atcgcagctc tgacgactca ctccttattt acccgggaaa
     2281 tacttctcca gcaatgcata caggatggca gcggtttcag catccattat gccgtcataa
     2341 tttttctgcc ggaaatgaag ctgaaaagcg cggacaagcg ccttatatcc gtcatccgtc
     2401 tccacaccag aaatactgta tccataggtc ttcagccgtg acacgatatc aaccttatcc
     2461 ggaatgccct gcgattcaaa aaataaatat ttagtcagta attttatgtt tttcctgaaa
     2521 aaattactct ttttcgaact ccagtgagcg ttcacgactc atcagtgtga aaatgatggc
     2581 aaaaacagca gacagtatca gtacgataaa tgtggccccc catcccagat aatcgacaac
     2641 aagtcccatt cccagtgtcg caacaattga gccgaagata taggaaaatg agcctgacag
     2701 ccctaacaga ataagtttac gcgtaaatcg cccggaatcc agaagagcat taagtatcac
     2761 ataaacaggt tccataccag tcctgaccgc atcaggacgc cagtaaataa aatctcgtag
     2821 tccaccggca tcagagacaa gagtaatcgt cgttaccggt atttcgactg gtttgtactg
     2881 gtgtgagcgg acaaaactac tgtacgtctc tcatacacgc tcgtaagcgg ccctcacggc
     2941 ccgctaacgc ggagatacgc cccgactgcg ggtaaaccct tgtcgggacc actccgaccg
     3001 cgcacagagg cattttcatc gctgacaggc cgcatggcct cgcagagccg gagggggact
     3061 ggtgatattc ggacgaccgt accggcttgc gccgggcttc ggcggtttta cacctgtgct
     3121 acatgtaacc accgtatgcc gccttatatg cgctgactcg cattatttgc cttgttccgt
     3181 ctgaaagtaa tcactatgat taaatatgat taacaactaa t
//
LOCUS       DAEEBB010000268         3220 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEEBB010000268 DAEEBB010000000
VERSION     DAEEBB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.272"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            8..511
                     /locus_tag="KLF07_004245"
     CDS             8..511
                     /locus_tag="KLF07_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD9834068.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="KLF07_004246"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="KLF07_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBD9834069.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="KLF07_004247"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="KLF07_004247"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBD9834070.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>3220)
                     /locus_tag="KLF07_004248"
     CDS             complement(2211..>3220)
                     /locus_tag="KLF07_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD9834071.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          893 a          719 c          675 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccagtat tcttacatca ttaatatatg gggattttaa aaaagtctcc
     2941 agaatcgaat cgatgaccgg tgtaaggtta acttttgcat catctaagga atcaataaga
     3001 atatgagaca ggttaacttt gatgtcatta atttcattgt cagtagcttt cttattatta
     3061 agaagataat tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca
     3121 agcaagtatg aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt
     3181 attgctaata atgcattgtt caatggtttt tctaataaat
//
LOCUS       DAEEBB010000269         3196 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEEBB010000269 DAEEBB010000000
VERSION     DAEEBB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.273"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="KLF07_004249"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="KLF07_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBD9834072.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="KLF07_004250"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="KLF07_004250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBD9834073.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="KLF07_004251"
     CDS             complement(1715..1984)
                     /locus_tag="KLF07_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBD9834074.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="KLF07_004252"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="KLF07_004252"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBD9834075.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="KLF07_004253"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="KLF07_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBD9834076.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          833 a          774 c          771 g          818 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatccggca tttttt
//
LOCUS       DAEEBB010000270         3174 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEEBB010000270 DAEEBB010000000
VERSION     DAEEBB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.274"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            532..2385
                     /locus_tag="KLF07_004254"
     CDS             532..2385
                     /locus_tag="KLF07_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBD9834077.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2536..2751
                     /locus_tag="KLF07_004255"
     CDS             2536..2751
                     /locus_tag="KLF07_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD9834078.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2756..3070
                     /locus_tag="KLF07_004256"
     CDS             2756..3070
                     /locus_tag="KLF07_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBD9834079.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          761 c          885 g          750 t
ORIGIN      
        1 ttccaggccc atccgctccg gtgggccttt ttactgcaga aaacaggttc cccgttaaat
       61 gctatgttgc tcacaattca gtaagttgac agttgcctgt cagactgggc atttgttaaa
      121 aaaatttcgc atggtgaatc cccctgagcg gaggggcgac tggtgacggt ataatctctg
      181 attatcaaaa cgagaatgac gcgggtttag tggcaccggg ctgaactcac cgggaggcac
      241 ccggcaccat gtgcatgatg atacagatac gcggctttag cccctctccg gaggggtttt
      301 cttgtgggca aaaaaagccc gcgctgggag acgcgggcgg caaggaataa acaataaaac
      361 gtgaagtaat atttcagctg gcgaataata ccccatagta atcactctgc gcaactgcgc
      421 ggtctttttc gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta acttccggac
      481 ttcagcccgc tccttatttt actcacaata ttatcccggc cgggaggatt catggcattt
      541 aaacactatg acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg actgacacaa
      601 aaactgaagg agggctggca gccgtttggt agtccggtgg ccataacccc ttataccctg
      661 atgcaggcga ttgcagcaga aggtgatgtg gtcgtcagtg gtgcaactga gccggagtgg
      721 tactacgtca tcgtactggc cgggcaatcc aatgccatgg cttacggtga aggtcttccg
      781 cttccggatt catacgatgc gccccatccg cgcattaagc aactggcccg tcgtaacaca
      841 gtgactcccg gtggtgaagt atgcgtattt aacgacatca ttcctgctga ccattgtctg
      901 catgatgttc aggatatgag tacgattaac catccccggg ctgacctgag caaagggcag
      961 tacggctgtg tcggacaggg cttacatatt gccaaaaaac tgcttccgta tatccctaat
     1021 aatgcgggga tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcacg
     1081 gaggggacat tcagcgagtc cacgggggcc agtcaggatt cggctcgctg gggagtgggt
     1141 aagccgttat atcaggatct gcttttccgc acgaaggcag cattgcagaa aaacccgaaa
     1201 aacgttttgc tggcgatatg ctggatgcag ggggaattcg atatgacgaa tgccagttac
     1261 gcccagcagc cagcagcatt tcttgcaatg gtacagcagt tccgtgctga ccttgccggg
     1321 ctggcggcgc agtgtcacgg tggaagtccg gcatcagtcc cctggatttg tggcgacacg
     1381 acatacgcgt ggaaacaaga acacggtacg caatatgaag tggtatatgg tgcatataaa
     1441 ggtaaagaat cccagcagat ttattttgtt ccctttatga ccgatggtag cggagttaat
     1501 acaccgacaa acaacccgtc agaagatcct gatattgccg ggtctggtta ttacggttcg
     1561 gcatcccgaa cgaacaaaaa ctgggtatca tcaaatcgcc cgacgcattt cagctcatgg
     1621 gcgcgtcgtg gcattattcc cgatcgtatg gcaactgcta ttctgaacgt agccggtcgc
     1681 accttagcct tcattagtgg taaggcaccg gaaatcaaac cctcgcccgg cggcgacacg
     1741 ccatcggggc cgtctgaaga tgcatccata cgcacaatct ccctgttgcc gacagccgga
     1801 gacgctgctg cgcagggctg gagcattaag aatggcggag ttcagttgtc agatggtgta
     1861 tttaagatca ccaagcagag caataaagcc tggtccctga cgcgcccggt ggatgacgca
     1921 gtctccctgc tgacacgggg tggcagactg agctgtaagt ttcgactgtc aggcgcactg
     1981 accaacaacc agttcggtct gggaatttat ctgtataccg atgtagcgtt acctgacgtc
     2041 gtggcgatga ccgggactgg taacccgttc ctgatgtcgt tcttcaccca gaccacagac
     2101 ggcaaactga atctgatgca tcacaagaaa gcaggaaaca caaagttggg cgagttcggg
     2161 aattacagta acgactggct gacgctggag ctggtgttca ccgccggcag tgccacggtt
     2221 actccgaaac tgaatggagt ggctggcccg gcattccagg tcataaaaga cagtctgaca
     2281 ctggggctga atgcgctgac gctgacggat attaccaaaa atgcagcgta tggcgttgag
     2341 atagaaagtc tggtgctgga gataaatgca ccggcatcat cataaaaagt gagccagcca
     2401 aatggaaggt atcgttaaac tcaccggtag tgtcagtggg tcgtctgaga tgcctgcatg
     2461 agttatcaga gccatcagta cttaactggt ggcttttttt attgttgtca gcttccggat
     2521 aacgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     2581 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     2641 tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata tctgactaac
     2701 ctgtatttca aaatcagaga ggaccgtcgt aaggctgcac ggggagagta attcaatgac
     2761 tcaaaactat gaactgattg tgaaagggat ccgcaatttt gagaataaag ttacggtaac
     2821 tttagcgtta cgggacaaaa aacgctttga cggtgaaatt tttgacctgg acatctcgct
     2881 ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca gcagccagaa ggagcatcag
     2941 acaggtcttc ctggatgttg ctgccgggtt atgtgaaggg gacgagctgt tgccagaaac
     3001 gcgcccctgt tcagaggcgc ggtataccat aaaaattaac agttctgata actcgattac
     3061 gggttgttag ctttttgcag ttggctttcc agtatctttc attggtagca tcctgataaa
     3121 tatccatgag cgcaaaaatc aaatacggcc tgtcagctgc tgttctggcg ctga
//
LOCUS       DAEEBB010000271         3142 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEEBB010000271 DAEEBB010000000
VERSION     DAEEBB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.275"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            61..576
                     /locus_tag="KLF07_004257"
                     /pseudo
     CDS             61..576
                     /locus_tag="KLF07_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            574..705
                     /locus_tag="KLF07_004258"
                     /pseudo
     CDS             574..705
                     /locus_tag="KLF07_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(799..1272)
                     /locus_tag="KLF07_004259"
                     /pseudo
     CDS             complement(799..1272)
                     /locus_tag="KLF07_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1445..2227
                     /locus_tag="KLF07_004260"
                     /pseudo
     CDS             1445..2227
                     /locus_tag="KLF07_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2805..>3142
                     /locus_tag="KLF07_004261"
     CDS             2805..>3142
                     /locus_tag="KLF07_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBD9834080.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLT"
BASE COUNT          669 a          892 c          840 g          741 t
ORIGIN      
        1 ggcacggaac cacggcagct tgacggctgc aatgacgtgt gtcgtctggc ggtggagtat
       61 ctgctgtatg cagcccgaaa acgggggctg gaaatcggca tcttctgcac catccacact
      121 ttacgccttc actttgaaga acatttaccc ctcgttgtag ccggacgtcg cctgggcgta
      181 ccaaaatcaa cagtttgtag tatgtttgtg cgcttccgca aagctggcct ttcatggcct
      241 ctgcccgcag gtatgtcgga gcgggaactt gacgcccgtc tttacgggag tgcctccaca
      301 gtacctgtcg tacttactga aagtaccgtt atgcccgaag tccccggggt aaaaaagcgt
      361 ccccggcgcc ccaacttccc ttatgagttc aaaatcgcct tagtggagca gtcactgcag
      421 cccggagcct gtgtggcgca gattgcccgt gaaaatggaa tcaacgataa cctgctcttc
      481 aactggcgcc atcaataccg gaaaggtggc ctgctgcctt ccggaaaaaa tatgccggca
      541 ctgcttcccg tgacgttaac gccggagccg gataaccatg gttttgacat catctatatg
      601 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgatccata cctgatcggc
      661 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      721 gtggtttggt gcctgcgcct gcacgccgac accgaggggc cttccctcat ctcttgtgca
      781 gcatggctgc acagggactc acgtccctgg tgcgcgcctt cgtggcacac catcatcggc
      841 gtacctttcg aagggaacgt cgggacactg acgttgcatc cacatatcaa aggcatagtg
      901 caggaacaga tttgagagca ggggcgagat cacgccgccc tgcggcgttc cttctgtcac
      961 cggccttacc agacctgcgt ccattacccc tgcattcagg aacctgcgga taagtgacaa
     1021 cacccgttta tccgatactc gtttctctat ccggctcatc agcacgtcgt gatttatccg
     1081 gtcgaagaac ttctccagat cgaggtcgac cacccagtga tacccggccc cgatatattc
     1141 ctgggcctgt atcactgcct ggtgggccga acgaccggga cggaacccgt agctgttgtc
     1201 actgaacgag ctatctcaca gtgcctgcaa tacctgcatc atagcctgct ggatgaagcg
     1261 atccaccacc atctggatgc ccagcagacg ttcgccgccg ccgggcttca gtatggatac
     1321 tcttctcaca ggggatgggc ggtatctgcc ggacagcagt tgcgctttca gctctggcca
     1381 gtggtgcctc aggtagtccg gcagttcact tacgcgcatg ccatcgactc ccgccgcacc
     1441 ttgattgcgt cttgtcgatg gaacagcaat cagcgcgccc gggggcggca gcgctgaatg
     1501 gcgactacat atgggatatg atcctcatac ctgtcagttc actgattttg agctaaccga
     1561 cagcagagac gctgaacggc tggatcgatt tgcgcaaacg gcagacgaga tacgaattgc
     1621 tgaccgggga ttcggttcgc gtcccgaatg tatccgctca cttgcttttg gagaagctga
     1681 ttatatcgtc cgggtttact ggcgaggatt gcgctggtta actgcagaag gaatgcgctt
     1741 tgacatgatg gattttctgc gcgggctgga ttgcggtaag aacggtgaaa ccactgtaat
     1801 gataggcaat tcaggtaata aaaaagccgg agctcccttt cctgcacgtc tcattgccgt
     1861 atcacttcct cccgaaaaag cattaatcag taaaacccga ctgctcagcg agaatcgtcg
     1921 aaaaggacga gtagttcagg cggaaacgct ggaagcagcg ggccatgtgc tattgctaac
     1981 atcattatcg gaagatgaat attcagcaga gcaagtggct gattgttacc gtctgcgatg
     2041 gcaaattgaa ctggctttta agcggctcaa aagtttgctg cacttggatg ctttgcgtgc
     2101 aaaggaactt gaactcgcga aagcgtggat atttgctaat ctactcgccg catttttaat
     2161 tgacgacata atccagccat cgctggattt tccccccaga agtgccggat ccgaaaagaa
     2221 gaactaacgc gttgtggaga ataacaaaaa tggtcatctg gagcttacag gtggccattc
     2281 gtgggacagt atccctgaca gcctacaaaa cgcaattgaa gaacgcgagg catcgtctta
     2341 acgaggcacc gaggcgtcgc gttcttcaga tggttcaacc cttaagttag cgcttatggg
     2401 gtaaatcccc gcctttttcc tgccggtccc cggacagagc acaaaggaag tcgccgccgg
     2461 ctccgcttta cccggccatg cggggcatgg ccttgtgggt tttcagctct gtagcctcag
     2521 cgtcgtgtgc gggctgtgcc gtgcctccat cttagccggg ctggcgttga tgcaagggta
     2581 cgcttcgccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt
     2641 cacccgctca gatttcgcgg tttcaccctc caactcccgc cagccgtgtg cgtcaggctg
     2701 cggcttccct tgcgccctgt gcatccccgc cttatcgccc ggctaagatg gaggcacggc
     2761 accgcccggt gtcgcggaat atgtgaacaa cggaggaaaa catcatgcaa tatgcgaaac
     2821 ctgtcactct gaatgttgaa gagtgcgacc gtctctcctt tctgccttac ctgtttggca
     2881 acgattttct gtatgccgag gcgtatgtgt acgcgctggc gcaaaaaatg atgccggaat
     2941 atcagggcgg cttctggcac ttcatccgcc tgccggacgg tggcggttac atgatgccgg
     3001 atggcgaccg tttccacatg gtgaacggtg caaactggtt tgaccgtacc gtgagtgctg
     3061 atgccgcagg catcattctg acttctcttg tgattaaccg ccagttgtgg ctgtaccacg
     3121 acagcgggga tgcaggactg ac
//
LOCUS       DAEEBB010000272         3109 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEEBB010000272 DAEEBB010000000
VERSION     DAEEBB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.276"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1255)
                     /locus_tag="KLF07_004262"
     CDS             complement(<1..1255)
                     /locus_tag="KLF07_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBD9834081.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="KLF07_004263"
     CDS             complement(1412..1621)
                     /locus_tag="KLF07_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834082.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="KLF07_004264"
     CDS             complement(1644..2354)
                     /locus_tag="KLF07_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9834083.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..>3109)
                     /locus_tag="KLF07_004265"
     CDS             complement(2870..>3109)
                     /locus_tag="KLF07_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834084.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          911 a          653 c          507 g         1038 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaaaaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttctatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccattcat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcctttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtc
//
LOCUS       DAEEBB010000273         3045 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEEBB010000273 DAEEBB010000000
VERSION     DAEEBB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.277"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..172
                     /locus_tag="KLF07_004266"
     CDS             <1..172
                     /locus_tag="KLF07_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834085.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="KLF07_004267"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="KLF07_004267"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBD9834086.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="KLF07_004268"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="KLF07_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="KLF07_004269"
     CDS             2190..>3045
                     /locus_tag="KLF07_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBD9834087.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          836 g          803 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcaccgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggagaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAEEBB010000274         2994 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEEBB010000274 DAEEBB010000000
VERSION     DAEEBB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.278"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            18..1076
                     /locus_tag="KLF07_004270"
     CDS             18..1076
                     /locus_tag="KLF07_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBD9834088.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="KLF07_004271"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="KLF07_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBD9834089.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="KLF07_004272"
     CDS             2805..>2994
                     /locus_tag="KLF07_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HBD9834090.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAEEBB010000275         2965 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEEBB010000275 DAEEBB010000000
VERSION     DAEEBB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.279"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..216
                     /locus_tag="KLF07_004273"
     CDS             <1..216
                     /locus_tag="KLF07_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBD9834091.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="KLF07_004274"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="KLF07_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="KLF07_004275"
     CDS             complement(415..597)
                     /locus_tag="KLF07_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9834092.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="KLF07_004276"
                     /pseudo
     CDS             696..1586
                     /locus_tag="KLF07_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="KLF07_004277"
     CDS             1589..2122
                     /locus_tag="KLF07_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD9834093.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="KLF07_004278"
     CDS             complement(2151..2678)
                     /locus_tag="KLF07_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD9834094.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2965)
                     /locus_tag="KLF07_004279"
     CDS             complement(2680..>2965)
                     /locus_tag="KLF07_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9834095.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          837 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgca
//
LOCUS       DAEEBB010000276         2944 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEEBB010000276 DAEEBB010000000
VERSION     DAEEBB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.280"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            45..1120
                     /locus_tag="KLF07_004280"
                     /pseudo
     CDS             45..1120
                     /locus_tag="KLF07_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1136..1513
                     /locus_tag="KLF07_004281"
     CDS             1136..1513
                     /locus_tag="KLF07_004281"
                     /inference="COORDINATES: protein motif:HMM:NF012565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD9834096.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKM"
     gene            1513..1884
                     /locus_tag="KLF07_004282"
     CDS             1513..1884
                     /locus_tag="KLF07_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD9834097.1"
                     /translation="MQVTMQHALKLFWRPKAIELEDDGVMSYEKVEIIRRNDGSIRFN
                     NKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVGYINDFGGLSF
                     YEINCPTVNNSCNVSVAKRDK"
     gene            1886..>2944
                     /locus_tag="KLF07_004283"
     CDS             1886..>2944
                     /locus_tag="KLF07_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834098.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAEEBB010000277         2937 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEEBB010000277 DAEEBB010000000
VERSION     DAEEBB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.281"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            35..262
                     /locus_tag="KLF07_004284"
     CDS             35..262
                     /locus_tag="KLF07_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBD9834099.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="KLF07_004285"
     CDS             complement(263..637)
                     /locus_tag="KLF07_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBD9834100.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="KLF07_004286"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="KLF07_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="KLF07_004287"
     CDS             complement(2097..2672)
                     /locus_tag="KLF07_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9834101.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttca
//
LOCUS       DAEEBB010000278         2919 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEEBB010000278 DAEEBB010000000
VERSION     DAEEBB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.282"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(94..1737)
                     /gene="yeeO"
                     /locus_tag="KLF07_004288"
     CDS             complement(94..1737)
                     /gene="yeeO"
                     /locus_tag="KLF07_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBD9834102.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1742..1817
                     /locus_tag="KLF07_004289"
     tRNA            1742..1817
                     /locus_tag="KLF07_004289"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Asn,seq:gtt)
     gene            complement(1855..2805)
                     /gene="cbl"
                     /locus_tag="KLF07_004290"
     CDS             complement(1855..2805)
                     /gene="cbl"
                     /locus_tag="KLF07_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBD9834103.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          855 a          781 c          615 g          668 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      121 acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      421 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
      661 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgcggg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccag
      901 ccctgccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
      961 aataataata ttaagaatat tcaggctacc gttaatcaat agcggtattt tcgtattccc
     1021 tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat aactgagtac
     1081 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     1141 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     1201 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     1261 gagactaaat gccacaacga cagtagtacc aagatcgata gcagcaaaaa aagccataat
     1321 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     1381 gaccagaaaa gtgctcagaa cccccatcaa caggacacag gcattctcca tgaagatagg
     1441 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     1501 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1561 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     1621 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1681 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaacagcga
     1741 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     1801 tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt tttgctatat
     1861 ctgataatca atttcctctt cactgttttc catcacctgc cgcttgatat cctcaactga
     1921 cagtcctgca ttacaaagtt ccagaaagcg ccagacatag ttacgctgaa gttgtcctcg
     1981 cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca gacggattaa
     2041 attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc caagcccaag
     2101 agcaacatag gttttaatga catcagaatc ctgcgcactt aatacaatat ctgccagcaa
     2161 acctttgcgg gcaaatgcgt catcaatacg tgagcgcccc gtaatcccct gtcggtaagt
     2221 gattaacggc cacttcgcta ttgattccag cgtcaatggt gtaatttgcg ttaagggatg
     2281 atcgagtgga acaagcaaac tatggtgcca acgaaaccac gggaaggcga cgagctgcgg
     2341 gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt gcaacaacgt
     2401 cgcaatttcc tgtggcgtcc cctggattag ctcgagccga acctccggga aaagttcgcg
     2461 aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt
     2521 gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat tactggcttc
     2581 attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca tgcccagcag
     2641 tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt cccgaatatg
     2701 acggctgacg ccggactgtg aggtaaaaag catattcgca acctctgtca ggttgtaatc
     2761 ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa actccgggca
     2821 gttcagattt cccgttattg ttaaagtcta atgcccggca taacaaataa taaaaacccg
     2881 catcttattc catcccgata taacgcttag ctcaccaat
//
LOCUS       DAEEBB010000279         2903 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEEBB010000279 DAEEBB010000000
VERSION     DAEEBB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.283"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..936
                     /locus_tag="KLF07_004291"
                     /pseudo
     CDS             1..936
                     /locus_tag="KLF07_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            1106..1384
                     /locus_tag="KLF07_004292"
     CDS             1106..1384
                     /locus_tag="KLF07_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBD9834104.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            1384..1761
                     /locus_tag="KLF07_004293"
     CDS             1384..1761
                     /locus_tag="KLF07_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834105.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            2230..>2903
                     /locus_tag="KLF07_004294"
     CDS             2230..>2903
                     /locus_tag="KLF07_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBD9834106.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYA"
BASE COUNT          963 a          469 c          684 g          787 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactgaat gcgtgactgc cattcttcag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctagatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 gaaaaatttc cgctgaaaga attaaataat ccagagcatg acagttacgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaaggactg aagaaattat gcgtggcagt ctcctttcgg
      601 tcaataatag cagaacaaaa gaaagagcca gaaatgacgg tcagatatta tatcagttct
      661 gctgatttaa ccgcagaaaa gttcgccaca gcaatccgaa accactggca cgtggagaat
      721 aagctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      781 aacgccgcag aattattttc agggatacgg cacatcgcta ttaatatttt aacgaatgat
      841 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga tagaaactat
      901 ctcgcgtcag tccttgcggg gagcgggctt tcgtaatctt tccctaggga gtcagccgaa
      961 tgttgggaag gatttgacgg atgaagagaa gaactgcttg gccggcggca gtacgggaag
     1021 cccgggagga tggggaccag aagatgagaa tagcgcaagg gatcgtatgc agcatgaacg
     1081 gtataaagat gaattgcgac agaaaatggg aaaacccgac gttacagatt caaatttgaa
     1141 aaatattata gacgatcttt atcggccaaa tgcaaaagta ggaagtggaa gcacagcaga
     1201 tgctgttcgt tatgaattgg ccacaggtga aaaagttggt ggtagaggac atgtattgaa
     1261 agcacaggat tatacaaggg ctttacagga ttggttggat aagaatccaa cagcatctcc
     1321 aggggatcga gcagcagcag aaaacgttct tcaggattta cagaatgctt tgggaggcaa
     1381 gtaatgaacc aatatattgc tagttatata gaaaaaatgg catcattttc cactgttttg
     1441 tctgagacag tctcattagc taatgaatac tggcatccag aaacgccacc ggtggttatg
     1501 ttgttctctc aggttggaaa aacgattgta aaaaattttt atgaaatggg ggatattgaa
     1561 agagaaaaaa tatttaaaca tgtcgaagat ggtatggtct cgcttgatga tgaattggcc
     1621 acagttgtag ctacagggct tatagaggca atagtcactg cgaccgatag caattcttta
     1681 ctttggcaag atttaagtaa atttctgggt gacaaatcaa agtctcatgc attagtgtgg
     1741 aaaaattttg gtcaggagtg atattattca agaagtatta agctcgctat tgagatctta
     1801 atgcttcttg agattaaatt aaagttagga cgaagtaatt gcaggtggaa ataaatccat
     1861 agtgacgtgt ggcaatggct attagatgtt atggtgtgat gggctttggt gatttaagag
     1921 ctaagttaga ttgccttatg aaaaagtatg gttatatatt taatgatgtg tatcatatat
     1981 ttaaataaaa aacggttctc tcttgtcaag atagtaatag gatagactat tgtgttgata
     2041 aatataatta agagtctact ttgttgattt tagaagactt tggagcaggg aaagatcatc
     2101 ggaagtacac tttttgtact aaataattcg cgctaaatgt tcaataattg agatattcct
     2161 tattacctaa aactattttt catagcttat atatgatcaa atactccttg cataaataag
     2221 gagaacaaaa tggaacttaa aaaactgatg gaacatattt ctattatccc cgattacaga
     2281 caagcctgga aagtagaaca taaattgtca gatattctac tgttgactat ttgtgccgtc
     2341 atttctggtg cagagggttg ggaagatata gaggattttg gtgaaacaca tctcgatttt
     2401 ttgaagcaat atggtgattt tgaaaatggt attcctgttc acgatactat tgccagagtt
     2461 gtatcctgta tcagtcctgc aaaatttcac gagtgcttta ttaactggat gcgtgactgc
     2521 cattcttcag atgataaaga cgtcattgca attgatggaa aaacgctccg gcactcttat
     2581 gacaagagtc gccgcaaggg agcgattcat gtcattagtg cgttctcaac aatgcacagt
     2641 ctggttctcg ggcagattaa gacggatgag aaatccaatg agattacagc cattcctgaa
     2701 cttcttaaca tgctggatat taaaggaaaa atcatcacaa ctgatgcgat gggatgccag
     2761 aaagatattg cagagaagat acaaaaacag ggcggtgatt atttattcgc ggtaaaagga
     2821 aaccaggggc ggcttaataa agcctttgag gaaaaatttc cgctgaaaga attaaataat
     2881 ccagagcatg acagttacgc aat
//
LOCUS       DAEEBB010000280         2893 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEEBB010000280 DAEEBB010000000
VERSION     DAEEBB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.284"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..493
                     /locus_tag="KLF07_004295"
     CDS             <1..493
                     /locus_tag="KLF07_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBD9834107.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="KLF07_004296"
     CDS             493..1083
                     /locus_tag="KLF07_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBD9834108.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="KLF07_004297"
     CDS             1070..2110
                     /locus_tag="KLF07_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD9834109.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="KLF07_004298"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="KLF07_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2893
                     /locus_tag="KLF07_004299"
     CDS             2682..>2893
                     /locus_tag="KLF07_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBD9834110.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          752 a          632 c          825 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagagcacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAEEBB010000281         2883 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEEBB010000281 DAEEBB010000000
VERSION     DAEEBB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.285"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..259)
                     /locus_tag="KLF07_004300"
                     /pseudo
     CDS             complement(1..259)
                     /locus_tag="KLF07_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            355..1356
                     /locus_tag="KLF07_004301"
     CDS             355..1356
                     /locus_tag="KLF07_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HBD9834111.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            complement(1532..1876)
                     /locus_tag="KLF07_004302"
     CDS             complement(1532..1876)
                     /locus_tag="KLF07_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HBD9834112.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKAFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            2462..>2883
                     /locus_tag="KLF07_004303"
     CDS             2462..>2883
                     /locus_tag="KLF07_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBD9834113.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAP"
BASE COUNT          686 a          755 c          743 g          699 t
ORIGIN      
        1 gatcgcattc aatcacgttg ttccggtact taatctgtcg gtgttcaacg tcagacgggc
       61 accggccttc gcgtttgagc agagcaagcg cgcgaccata ggcgggcgct ttatccgtgt
      121 tgatgaatcg cgggatctgc cacttcttca cgttgttgag gattttaccc agaaaccggt
      181 atgcagcttt gctgttacga cgggaggaga gataaaaatc gacagtgcgg ccccggctgt
      241 cgacggcccg gtacagatgg ttctgttttc taatgctaag ctgcaaacca gctgcccatg
      301 ccgtcaatac agatgcaccg atgcgcttgc gtggcttttc agggagataa agttatggcc
      361 gatccattca atctgcagac cgatgtggtc aggcagcata cggttccccg ttttttactg
      421 aagcatttca gtacgccggg aaagggtaag cggcagcgct tatacgcctt tgacaaggct
      481 gccgggcgcg cctatgccac cacgccggat gacgcgaccg tccgcaacac gttctacaac
      541 ctcgataatc acccggaccg tctgagtctt gaaccactgc tggggattta cgagcaccac
      601 gcggcaccgg taattgcagc cctgctggcg cacagggata ttcgcaggct gacagatgat
      661 gaacgctaca ggctggccgt gtttgttgcc gtgcagcgcg cacgaacctt cggtgaactg
      721 gagcgtatct caggcatgat ttcggtgctt accgataaga tggaggccat cggctcgact
      781 aaagagcagg ccatggaaac cctgggcctg tcgtccgggg gcgataccaa ggatattttt
      841 ctgcggcagc tcgtccagca ggtgtcacat attgatctcc tgctgaaaaa ggactggtac
      901 cttctggaaa ccaggccaga acgtccgttt tacgtatcag acaatcccgt agtgcttaag
      961 aacagcaatg acttcggtcc gtacggtaat cttggtctgg ccgttcgcgg gatacagatt
     1021 tacctccccc tgtcatcgac gctgatgctg gccatgtatt gcccctccat ccgtgaacag
     1081 atggttcgtc aaaagcagca tcttcaacat ctgctcgcca gggcgccgca tcttatcccc
     1141 cgtcacattc ggccttttga aagactggaa catatccgga ggtatacgga ctatcttctt
     1201 atgccgctta ccccggagca tgtcacgcat tacaattccc tgcaggtgga gttcgcggag
     1261 cagtacgtgt tctgcggtga aaaggatttc tctctggttg agagaatgct ggccgatagt
     1321 gaacgctacc ggactggccc gcggtttacg ttttagtccg aattccggcc tcacctgtgc
     1381 cggaaaatga cggcgggagc ccctgttgta cagaggagtt ttgccgtgtt gcacccacgc
     1441 gaaagcggca gtggtcacag ggaccaccgc cgtaaggaga tggtgagtat ctgttgacat
     1501 tcaccatcag ttcgtgtagc gttgcggcta cttactttag cagagtaaat cttcggcaag
     1561 tggcggtaac cactttaccg ataccccaga gtagtccgcg ctttactggc gtgacccata
     1621 gtgccgaaac cgcaaatttc gacactataa aggccttcag ccagggcact acaaaccaga
     1681 ccaccgcagc acagagaccc acataggcca taagaagcaa aatacagaga caggcgctcc
     1741 agtattgctt ctggcaaata tggtccctgg ttccgataac caggcgttta acggtggaga
     1801 aaatcgtcca aataaaatgg atttcagtct ggaatgggaa tagaaccgtt ccctcggtgt
     1861 ctaacaagtt tttcatagaa ccctcccggg taagtattta caaaagtaac tgtcttgatt
     1921 ttggtcatct caataacctc gtttttaagt caaaaggcac ccggcgcctc acacacgtga
     1981 acgctgttaa tgccgtacag aatgttttat ctttcagctc tgttatgatg tgttcattcg
     2041 catcctcctg tttgtcatac cgattttaag ctcgttccgg gggctccccg gaaaaaaatc
     2101 caaaaaaatc cgggccggaa tggtgataac cagtctggcg ggaaataaac ggatcttgtt
     2161 ggggtggagc gggacggaac aatactatga gagtaatgtt aacagaaatc accgcctttg
     2221 tcaaggcgca cttatccatt aaagcaacta tagcatagta atactgatgt ccatttagca
     2281 aaaagtgcta tccgtcgttc tgctgtatgt ctattttcct tcacctccgg gcaacctggc
     2341 gaatacattt ttactttttt tgccccctgc cccctcagag taccgagacg aagagatggg
     2401 cactgttgca aatagtcggt ggtgataaac ttatcatccc cttttgctga tggagctgca
     2461 catgaaccca ttcaaaggcc ggcattttca gcgtgacatc attctgtggg ccgtacgctg
     2521 gtactgcaaa tacggcatca gttaccgtga gctgcaggag atgctggctg aacgcggagt
     2581 gaatgtcgat cactccacga tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa
     2641 acggctgcgc tggtactggc gtaacccttc cgatctttgc ccgtggcaca tggatgaaac
     2701 ctacgtgaag gtcaatggcc gctgggcgta tctgtaccgg gccgtcgaca gccggggccg
     2761 cactgtcgat ttttatctct cctcccgtcg taacagcaaa gctgcatacc ggtttctggg
     2821 taaaatcctc aacaacgtga agaagtggca gatcccacga ttcatcaaca cggataaagc
     2881 gcc
//
LOCUS       DAEEBB010000282         2879 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEEBB010000282 DAEEBB010000000
VERSION     DAEEBB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.286"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="KLF07_004304"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="KLF07_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBD9834114.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="KLF07_004305"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="KLF07_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBD9834115.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="KLF07_004306"
     CDS             1649..2098
                     /locus_tag="KLF07_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD9834116.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /locus_tag="KLF07_004307"
     CDS             complement(2099..2332)
                     /locus_tag="KLF07_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBD9834117.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="KLF07_004308"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="KLF07_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBD9834118.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2879
                     /gene="yncL"
                     /locus_tag="KLF07_004309"
     CDS             2786..>2879
                     /gene="yncL"
                     /locus_tag="KLF07_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBD9834119.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          758 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttattt
//
LOCUS       DAEEBB010000283         2838 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEEBB010000283 DAEEBB010000000
VERSION     DAEEBB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.287"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1118..1453)
                     /locus_tag="KLF07_004310"
     CDS             complement(1118..1453)
                     /locus_tag="KLF07_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBD9834120.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1582..1929
                     /locus_tag="KLF07_004311"
     CDS             1582..1929
                     /locus_tag="KLF07_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834121.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1949..2539)
                     /locus_tag="KLF07_004312"
     CDS             complement(1949..2539)
                     /locus_tag="KLF07_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834122.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(2536..2796)
                     /locus_tag="KLF07_004313"
     CDS             complement(2536..2796)
                     /locus_tag="KLF07_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834123.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          795 a          671 c          588 g          784 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg
      481 gggattacat ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt
      541 aatataagct tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt
      601 atattataaa tataacataa tacatctata tggggtgtac atatccccac ataaaaacac
      661 atactctcat taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc
      721 gttcttgtac tatggtttag tattctcaag tacttacaga gaaagaatag catattacat
      781 cctttatttt cactaatcct gacaccacta accattttaa tcattagact tatgatagat
      841 ttaacataca accaataaat tataaacaaa ataaaaatag ggaacattat atgctttata
      901 aaattttcac attaaacaca aagataaaaa caactatcat tacaatatgt gcattatcat
      961 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
     1021 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
     1081 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
     1141 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
     1201 tataaacagc aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga
     1261 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
     1321 aaaccattcc gcagagagaa atacaccaga acagtaatct gtatttaaaa tcataatccc
     1381 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
     1441 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
     1501 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
     1561 tcatggaacg gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg
     1621 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
     1681 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
     1741 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
     1801 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga
     1861 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat
     1921 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
     1981 gaacctccat tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc
     2041 cctcccggcg taccacccag ccttctttat tccataactg cagacaggtg ctgccgtcga
     2101 gacgcaccac aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca
     2161 gatcagtggc agaagtgccg cctgcgggca gacctgctgc cgggaccagc ggcagtacga
     2221 cgacttctgc cgcacgctgt tctgcccgga tgcggtcctg ttctgccagc agcagaggct
     2281 gtgccacggc ccggccggcg tccgtcagct ccactgccag ctgcaggttc ggagcgcgca
     2341 gggtacgcag ccagccggca tcctccagac gacggcagga ggcgcgcagg tttggcccgt
     2401 agaccggggc ttccccgctt tgctccagca cccgttctat gtcccgcgtc agcacgggtc
     2461 ctggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg
     2521 gtcggcggct ctgagtcatt tccagtcatc ctccggccag atgagctttc tgaggcgctg
     2581 atggtcggcg tcaatgatct cctgtctctt tatggcattc attgaggtat agccatttat
     2641 ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataagt
     2701 tacgccgtgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg
     2761 ttcatagctg agtgaaaacg tcatttcctg atccatcctg cacgttccat ctgataatgt
     2821 tctgacaggt aaaaatca
//
LOCUS       DAEEBB010000284         2797 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEEBB010000284 DAEEBB010000000
VERSION     DAEEBB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.288"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..617
                     /locus_tag="KLF07_004314"
                     /pseudo
     CDS             1..617
                     /locus_tag="KLF07_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            614..1093
                     /locus_tag="KLF07_004315"
     CDS             614..1093
                     /locus_tag="KLF07_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBD9834124.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="KLF07_004316"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="KLF07_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBD9834125.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2467..>2797
                     /locus_tag="KLF07_004317"
     CDS             2467..>2797
                     /locus_tag="KLF07_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834126.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          620 c          590 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttccctta tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAEEBB010000285         2739 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEEBB010000285 DAEEBB010000000
VERSION     DAEEBB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.289"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="KLF07_004318"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="KLF07_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBD9834127.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="KLF07_004319"
     CDS             1322..2374
                     /locus_tag="KLF07_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBD9834128.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAEEBB010000286         2727 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEEBB010000286 DAEEBB010000000
VERSION     DAEEBB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.290"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(423..680)
                     /locus_tag="KLF07_004320"
     CDS             complement(423..680)
                     /locus_tag="KLF07_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834129.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="KLF07_004321"
     CDS             complement(677..1174)
                     /locus_tag="KLF07_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBD9834130.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="KLF07_004322"
     CDS             complement(1376..1834)
                     /locus_tag="KLF07_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD9834131.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="KLF07_004323"
     CDS             complement(1831..2328)
                     /locus_tag="KLF07_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD9834132.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="KLF07_004324"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="KLF07_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBD9834133.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAEEBB010000287         2721 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEEBB010000287 DAEEBB010000000
VERSION     DAEEBB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.291"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            30..2546
                     /locus_tag="KLF07_004325"
     CDS             30..2546
                     /locus_tag="KLF07_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD9834134.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="KLF07_004326"
     CDS             2557..>2721
                     /locus_tag="KLF07_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9834135.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAEEBB010000288         2690 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEEBB010000288 DAEEBB010000000
VERSION     DAEEBB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.292"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            230..769
                     /locus_tag="KLF07_004327"
     CDS             230..769
                     /locus_tag="KLF07_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBD9834136.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KLF07_004328"
     CDS             complement(995..1318)
                     /locus_tag="KLF07_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBD9834137.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KLF07_004329"
     CDS             1604..1798
                     /locus_tag="KLF07_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD9834138.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAEEBB010000289         2644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEEBB010000289 DAEEBB010000000
VERSION     DAEEBB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.293"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            12..143
                     /locus_tag="KLF07_004330"
     CDS             12..143
                     /locus_tag="KLF07_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBD9834139.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="KLF07_004331"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="KLF07_004331"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBD9834140.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="KLF07_004332"
     CDS             complement(1439..2392)
                     /locus_tag="KLF07_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD9834141.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAEEBB010000290         2556 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEEBB010000290 DAEEBB010000000
VERSION     DAEEBB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.294"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="KLF07_004333"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="KLF07_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBD9834142.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="KLF07_004334"
     CDS             complement(390..1178)
                     /locus_tag="KLF07_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBD9834143.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="KLF07_004335"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="KLF07_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBD9834144.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="KLF07_004336"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="KLF07_004336"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD9834145.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="KLF07_004337"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="KLF07_004337"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBD9834146.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAEEBB010000291         2551 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEEBB010000291 DAEEBB010000000
VERSION     DAEEBB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.295"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..348
                     /locus_tag="KLF07_004338"
     CDS             <1..348
                     /locus_tag="KLF07_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9834147.1"
                     /translation="NVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTS
                     QRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGRP
                     LKQEPTEMIQATA"
     gene            complement(388..1059)
                     /locus_tag="KLF07_004339"
     CDS             complement(388..1059)
                     /locus_tag="KLF07_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBD9834148.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1166..1399)
                     /gene="yedF"
                     /locus_tag="KLF07_004340"
     CDS             complement(1166..1399)
                     /gene="yedF"
                     /locus_tag="KLF07_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBD9834149.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1396..2550)
                     /gene="yedE"
                     /locus_tag="KLF07_004341"
     CDS             complement(1396..2550)
                     /gene="yedE"
                     /locus_tag="KLF07_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBD9834150.1"
                     /translation="MPAVIAAGILSTYYFGITGTFWAVTGEFTRWGGQLLQLFGVHAE
                     EWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVKLRMPRSRIRIMQAIIGG
                     IIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSWFGARFTLLPIFRIPVKM
                     QKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQPKLGLAMLFGVGFGLLI
                     ERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYVQLGVEPKIMWAGPNAVI
                     GGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGSTILAYYWDDFAPALATD
                     WDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRAAPQTAKEIA"
BASE COUNT          651 a          694 c          673 g          533 t
ORIGIN      
        1 aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg gtatgaaatg
       61 cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc tgttccgcca
      121 gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa ccgcctgtca
      181 caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt aaatggcgct
      241 cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt gcagtcaggc
      301 cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac gtagcaggga
      361 tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact ccgcgccctg
      421 gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt ttgctggcac
      481 acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc gcatccattc
      541 ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta ccagaccctg
      601 gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct caaatggtgt
      661 gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa aaaaaggctg
      721 ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga ttgcccgacc
      781 atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc gggcgttaat
      841 cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat ccagatgaag
      901 gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt tgtatctgcc
      961 aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa gcgcaaggta
     1021 atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct gagatcatac
     1081 tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca atgttcccgc
     1141 tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg gtcggcccgt
     1201 cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga atattattga
     1261 tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac tgcggcatcg
     1321 cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca aggcggtaat
     1381 caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac ggcggaagaa
     1441 gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat atgtcaccag
     1501 caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt cggtggccag
     1561 cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca cattgcccag
     1621 accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc cggtttcgca
     1681 gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg cgtttggtcc
     1741 cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc cgatggcact
     1801 caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga tccacatatc
     1861 gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa agccgacgcc
     1921 aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg cccagcccag
     1981 catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat ccggtttttg
     2041 cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga agatgggcag
     2101 cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa agaaccaggc
     2161 atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc ccattgccag
     2221 acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc ggatacggct
     2281 gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc agccgccaaa
     2341 catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca gatggataat
     2401 tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct gaccgcccca
     2461 acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat agtaagtgga
     2521 gagaataccc gccgcgatga ccgcagggat g
//
LOCUS       DAEEBB010000292         2539 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEEBB010000292 DAEEBB010000000
VERSION     DAEEBB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.296"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="KLF07_004342"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="KLF07_004342"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBD9834151.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="KLF07_004343"
     CDS             complement(1522..2031)
                     /locus_tag="KLF07_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBD9834152.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          659 c          579 g          659 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccct
//
LOCUS       DAEEBB010000293         2472 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEEBB010000293 DAEEBB010000000
VERSION     DAEEBB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.298"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..713)
                     /locus_tag="KLF07_004344"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="KLF07_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="KLF07_004345"
     CDS             complement(766..1362)
                     /locus_tag="KLF07_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9834153.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="KLF07_004346"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="KLF07_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBD9834154.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="KLF07_004347"
     CDS             complement(2006..>2472)
                     /locus_tag="KLF07_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9834155.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          679 a          537 c          496 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagcg gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DAEEBB010000294         2446 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEEBB010000294 DAEEBB010000000
VERSION     DAEEBB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.299"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(25..315)
                     /locus_tag="KLF07_004348"
     CDS             complement(25..315)
                     /locus_tag="KLF07_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBD9834156.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="KLF07_004349"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="KLF07_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBD9834157.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="KLF07_004350"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="KLF07_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBD9834158.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="KLF07_004351"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="KLF07_004351"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2446)
                     /locus_tag="KLF07_004352"
     CDS             complement(2171..>2446)
                     /locus_tag="KLF07_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD9834159.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          575 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DAEEBB010000295         2403 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEEBB010000295 DAEEBB010000000
VERSION     DAEEBB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.300"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="KLF07_004353"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="KLF07_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBD9834160.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="KLF07_004354"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="KLF07_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="KLF07_004355"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="KLF07_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          667 a          505 c          618 g          613 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 cta
//
LOCUS       DAEEBB010000296         2329 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEEBB010000296 DAEEBB010000000
VERSION     DAEEBB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.301"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            20..238
                     /locus_tag="KLF07_004356"
     CDS             20..238
                     /locus_tag="KLF07_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBD9834161.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="KLF07_004357"
     CDS             240..605
                     /locus_tag="KLF07_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD9834162.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="KLF07_004358"
     CDS             602..1267
                     /locus_tag="KLF07_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834163.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="KLF07_004359"
     CDS             1267..1632
                     /locus_tag="KLF07_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBD9834164.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="KLF07_004360"
     CDS             2087..2242
                     /locus_tag="KLF07_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD9834165.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          496 c          628 g          578 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DAEEBB010000297         2229 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEEBB010000297 DAEEBB010000000
VERSION     DAEEBB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.302"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(64..>2229)
                     /gene="traD"
                     /locus_tag="KLF07_004361"
     CDS             complement(64..>2229)
                     /gene="traD"
                     /locus_tag="KLF07_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBD9834166.1"
                     /translation="RIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYW
                     WCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVA
                     LVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIGDL
                     PIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSID
                     KILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLM
                     RNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAI
                     RYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGL
                     LAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEK
                     AAATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKD
                     MERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMEN
                     RLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQPQ
                     QPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAY
                     EAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          520 a          628 c          545 g          536 t
ORIGIN      
        1 taacacccgg gaatttgaac gtgtgggcgg cctgagaact gaagactgga gctgacctgt
       61 tcatcagaaa tcatctcccg gctcaacatc ctccccgcgt tcccggtgca cattgatgtt
      121 cacctcttca cgacgctgca tctgctgcca ggtgtccgga tgattttcct gctgccatgc
      181 ctcataagcg gccatatcca ccacttcacc ggattcactg atcccgggtg gcagttgctg
      241 ttccatctct tcttccggtt tcattttcag ctcctgctca atcccccctg ccggaacatt
      301 cacacctgaa tctgacttct tatcgttgat gacaggagac accggctgtt gcggctgttg
      361 cggctgttgc ggctgttgcg gctgttgcgg ctgttgcggc tgttgcggct gttgcggctg
      421 ttcagcctga gtcacgtctt ctccggaaac aacctccggg acatccggtt cgaagaggct
      481 ggccatctga cgaccttctg cttcccttgc ggcaagtacg gcactcagac ggttctccat
      541 ttccgggttg atgtcacgcg gaataaactc cggagcgacc ttcggacgtt cctgatattt
      601 cagtgagagt ttgactgccg gatacggtcc gggcagggtg acatagcagg tcagatccgg
      661 cagggactga atgtcggaat aactgaccag cgtctggcgc tccatatcct taccggtcga
      721 taccccgtca cgtaccggat cagcaccgta ggaatactgt tcgctggctt tcaggtgctc
      781 tttctcacca atttcaccga cagcgaactc cgcaatctta tggctgggag aacggaaaaa
      841 ggcacgggtg ttcatgacgt caaacagcgt ggcagccgct ttctcaccgt agatatcttc
      901 cagctgggca taggactgga taccaaacac ataacagcca ccgaacttac gggcttccgg
      961 caggatctcc accaggtccg gcagtttgtg taatgtgggt aactcatcac agaagaacca
     1021 cacacgacga ttacggtttt ctcccattgc cagcagacca cgaatggcaa tggacagcca
     1081 catggagatc accggcttca gggaggcatg agtgtcggca ttcgacgaga taaacagcca
     1141 gccgtttttc tgatcctccc ggacaccacg catccagtca cggatggtga agggctcacc
     1201 gttatgctca atcccctgca ggtaacggat ggctttcaca tagttggtca gcacagcacg
     1261 gatggaaatc gccgttttct caattttctc ttccaccagg ttggccgccg gtgaattacg
     1321 caggtaggta cgcagttttt caattttgat ggaaagcagt gtgtccacca gtttgctgta
     1381 gctgcggttg gggtcattac gcatcagata cgccgcttcc gcaaaaatgg tacgtcctga
     1441 accctgccag aacgggtcct cttttgtgcc catcgggatc agagtatttg cggtattatc
     1501 aaaatccggc tgtgtcaggc actccttcca caaatcccag gcggcacagc gtgcatccag
     1561 cggattcagg atcttatcga tggagggatc atagtaactt ttaacaaatt cccctgaacg
     1621 gtcataaatc accaccatat caccacgctg acgggcgtag ttggccagac gacggataac
     1681 ctccgactta ccggccccga ccgtgccgtg cagacagaag ttctggattt cggaatcccg
     1741 gataatcggc agatcgccaa tccggatatc ggagtccctg ccgtcttttt tcagcatccg
     1801 ggcaacgtct ttcggattgt ctgtcagttg acgaccacct gtgacttcat tttcgctctg
     1861 ttgtttaccc tgacgcccca gaatccagga gacaacaaag aaggtaatca ggcaaataac
     1921 cagtgccaca actgtggcca gaacgaatgc agaccataac tgctcaccgc accagatcat
     1981 atatttatca tgcagtacct gagcagcgtt catccggaag gttttgccgt aatactgaat
     2041 ctcatatacc ggctgagact tgattaaatc ccgcatgcct tccagcgtgg tacaccacca
     2101 gtaaatacag ccattcacaa acgtctgcca gcttattttt acccataaaa ccagaccaac
     2161 gagtatccag aaaaaaataa acaggcagta aagcataata ttggcgatct ggctgaacat
     2221 gcggatacg
//
LOCUS       DAEEBB010000298         2204 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEEBB010000298 DAEEBB010000000
VERSION     DAEEBB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.303"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1919
                     /gene="fusA"
                     /locus_tag="KLF07_004362"
     CDS             <1..1919
                     /gene="fusA"
                     /locus_tag="KLF07_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBD9834167.1"
                     /translation="ATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAV
                     MVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGANPVP
                     LQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESA
                     AEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVID
                     YLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGV
                     VNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDP
                     DAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTII
                     AGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYG
                     HVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDM
                     GIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENTGDVI
                     GDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEA
                     PSNVAQAVIEARGK"
     gene            1990..>2204
                     /gene="tuf"
                     /locus_tag="KLF07_004363"
     CDS             1990..>2204
                     /gene="tuf"
                     /locus_tag="KLF07_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD9834168.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
BASE COUNT          581 a          535 c          578 g          510 t
ORIGIN      
        1 ctgcgactac tgcattctgg tctggtatgg ctaagcagta tgagccgcat cgcatcaaca
       61 tcatcgacac cccggggcac gttgacttca caatcgaagt agaacgttcc atgcgtgttc
      121 tcgatggtgc ggtaatggtt tactgcgcag ttggtggtgt tcagccgcag tctgaaaccg
      181 tatggcgtca ggcaaacaaa tataaagttc cgcgcattgc gttcgttaac aaaatggacc
      241 gcatgggtgc gaacttcctg aaagttgtta accagatcaa aacccgtctg ggcgcgaacc
      301 cggttccgct gcagctggcg attggtgctg aagaacattt caccggtgtt gttgacctgg
      361 tgaaaatgaa agctatcaac tggaacgacg ctgaccaggg cgtaaccttc gaatacgaag
      421 atatcccggc agacatggtt gaactggcta acgaatggca ccagaacctg atcgaatccg
      481 cagctgaagc ttctgaagag ctgatggaaa aatacctggg tggtgaagaa ctgactgaag
      541 cagaaatcaa aggtgctctg cgtcagcgcg ttctgaacaa cgaaatcatc ctggtaacct
      601 gtggttctgc gttcaagaac aaaggtgttc aggcgatgct ggatgcggta attgattacc
      661 tgccatcccc ggttgacgta cctgcgatca acggtatcct ggacgacggt aaagacactc
      721 cggctgagcg tcacgcaagt gatgacgagc cgttctctgc actggcgttc aaaatcgcta
      781 ccgacccgtt tgttggtaac ctgaccttct tccgtgttta ctccggtgtg gttaactctg
      841 gtgataccgt actgaactcc gtgaaagctg cacgtgagcg tttcggtcgt atcgttcaga
      901 tgcacgctaa caaacgtgaa gagatcaaag aagttcgcgc gggcgacatc gctgctgcta
      961 tcggtctgaa agacgtaacc actggtgaca ccctgtgtga cccggatgcg ccgatcattc
     1021 tggaacgtat ggaattccct gagccggtaa tctccatcgc agttgaaccg aaaaccaaag
     1081 ctgaccagga aaaaatgggt ctggctctgg gccgtctggc taaagaagac ccgtctttcc
     1141 gtgtatggac tgacgaagaa tctaaccaga ccatcatcgc gggtatgggc gaactgcacc
     1201 tcgacatcat cgttgaccgt atgaagcgtg aattcaacgt tgaagcgaac gtaggtaaac
     1261 cgcaggttgc ttaccgtgaa actatccgcc agaaagttac cgatgttgaa ggtaaacacg
     1321 cgaaacagtc tggtggtcgt ggtcagtatg gtcatgttgt tatcgacatg tacccgctgg
     1381 agccgggttc aaacccgaaa ggctacgagt tcatcaacga cattaaaggt ggtgtaatcc
     1441 ctggcgaata catcccggcc gttgataaag gtatccagga acagctgaaa gcaggtccgc
     1501 tggcaggcta cccggtagta gacatgggta ttcgtctgca cttcggttct taccatgacg
     1561 ttgactcctc tgaactggcg tttaaactgg ctgcttctat cgcctttaaa gaaggcttta
     1621 agaaagcgaa accagttctg cttgagccga tcatgaaggt tgaagtagaa actccggaag
     1681 agaacaccgg tgacgttatc ggtgacttga gccgtcgtcg tggtatgctc aaaggtcagg
     1741 aatctgaagt tactggcgtt aagatccacg ctgaagtacc gctgtctgaa atgttcggat
     1801 acgcaactca gctgcgttct ctgaccaaag gtcgtgcatc atacactatg gaattcctga
     1861 agtatgatga agcgccgagt aacgttgctc aggccgtaat tgaagcccgt ggtaaataag
     1921 cctaagggtt aataccaaag tcccgtgctc tctcctgaag gggagagcac tatagtaagg
     1981 aatatagccg tgtctaaaga aaaatttgaa cgtacaaaac cgcacgttaa cgttggtact
     2041 atcggccacg ttgaccacgg taaaactact ctgaccgctg caatcaccac cgtactggct
     2101 aaaacctacg gcggtgctgc tcgtgcattc gaccagatcg ataacgcgcc ggaagaaaaa
     2161 gctcgtggta tcaccatcaa cacttctcac gttgaatatg acac
//
LOCUS       DAEEBB010000299         2202 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEEBB010000299 DAEEBB010000000
VERSION     DAEEBB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.304"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..219)
                     /locus_tag="KLF07_004364"
     CDS             complement(<1..219)
                     /locus_tag="KLF07_004364"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD9834169.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPL"
     gene            complement(216..869)
                     /locus_tag="KLF07_004365"
     CDS             complement(216..869)
                     /locus_tag="KLF07_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBD9834170.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(880..2139)
                     /locus_tag="KLF07_004366"
     CDS             complement(880..2139)
                     /locus_tag="KLF07_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBD9834171.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT          491 a          685 c          569 g          457 t
ORIGIN      
        1 cagcggatac ggttctacct ctttccacgc cagattctgt aacgccagca gccccacctg
       61 cggcccgttg ccgtgaacaa tcgccagccg ataagaacgg gccaggcgtg ccagcgcggg
      121 tacagcactg gcgatattgc gatattgatt ttccgccgtc agcgcctcac cgcgctggag
      181 taaggcgttg cccccaagag ctacaaccag tgttttcatg gttttccttg taataattgt
      241 tggccaagcc agaaccccag cagcgtatct gcccctgacg tatgccccag cgccagcagc
      301 gaatcaatcg caacagcggt acgtttcggg caactcagag catggacaaa gtgcaggagt
      361 ggcgaggcga aatatccttg cgcggcataa cgtaaataac tgacgctcac cgctgtggta
      421 acgagttgaa gattgtcgga ataggcaaaa aactggcgta actcagcagg cagcggatcg
      481 cctgccgcca acgccagtgg cccgaaaagc ccggtttcct ccgcgcgttg catccatgca
      541 gctgcaagcg gttgaggatg cgcaggcggc gtaatacgca gcaaacaata acgctgtggc
      601 tgtttaaccg tgaaacgccc gaggcgaatc ccttgagcca caacctgtgg tcgttcattg
      661 ccgcataatc caccgcataa cgcatcgaat tgcgcgcggc gaagcaccca tcctccgggg
      721 ccgaaaccac taccctgacg atgcagcgtt aacagttcgc cgctttccgt catcaggtta
      781 atcgcccgtc gccacactcc cgctaaccgc caggactggc gataattcgg tgcactacgg
      841 ctggcaagca gaggatggat gatcgtcatt gcgcgttcct caaccaatcc ccatgctttc
      901 agccagtgct tccagcgcct gttcaaaaca cgccagcggt gcccgcacag tgcctgcgcc
      961 aatctgcccg atccccgcct ctttatgggc gataccggta ttgatgagtg gcgtaatgtc
     1021 ggtttctacc acgcgacgaa tgtccagtcc caggcacgcg ccctgaaaat cccagcctgg
     1081 gatctgcaac tgcatattgc gctcaaggta aatttccgcc atctcttcag ataccgctct
     1141 ggccgcttcc atgccacccg caccgacaaa gcgcgttacg ccaggcgctg cgatcattgc
     1201 cgcacctccg ataccaaagg tttcggtaat cgcgctatcg cccatatccg ggttcgcctg
     1261 ctcctgcgag aagccggtga aaaacagacc ttgcggagtg tttacaggcg cagtaaacca
     1321 gcgttcgccc agcccgctta cccgaatccc gaacatattg ccgttgcggg tcattgccgt
     1381 gacgatgctg cctgcgcgga tcatcgcgcc agcatccatc gccgccttgc agtaagccat
     1441 cgcgaggttg aggaagaact gatcggtcac gctgaggaaa tccatcactt cggcgatgtg
     1501 ctgtttatca tgatcgaggc gagcaatttg tggggccagc gcacgcatta acagtgcaga
     1561 ggaagcaata ttgcgttgat ggaactcatc gcccatcgta atgccctgcg ccatcatcgc
     1621 cgtgagatcg ataccgcgct ccatgcgccc cagcgccgcg cttaatactg gcattaacac
     1681 atcgcgcatc cagcgatggc gagtcaggac atcttcgccg taagcgccaa aacgcatcac
     1741 tttgccgata ccttcgttga ggttgcagta cgcccggttg ccgtcggtca cgttctcgac
     1801 caccagcatc ggcatactgg cagaagtaat accgcccatt ggaccgacgg cattcacatg
     1861 gtgacaagga atgaagttca cttccccctg ctccagtatt gccagcgcct gcgcttcatc
     1921 tttcgcccag ccttcgaaca gacatgcgcc cacgcacgcc cctttcatgg gtccggtcat
     1981 ctcctgccag cgcattggcg gcccggcgtg aagcagtgtt ttgccctcgt ttagttcgct
     2041 gataagtgaa gaagccggtt gcacatccag ccagtgtgga cgagcgcgac gaatttgttc
     2101 gataaccgca gcattggctt gcgccactga tgtaaacatg ggaacccctt attgcaaacg
     2161 ttctaataaa cgagccaggt aatgactcca acttattgat ag
//
LOCUS       DAEEBB010000300         2175 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEEBB010000300 DAEEBB010000000
VERSION     DAEEBB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.305"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(309..1088)
                     /gene="ipaJ"
                     /locus_tag="KLF07_004367"
     CDS             complement(309..1088)
                     /gene="ipaJ"
                     /locus_tag="KLF07_004367"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBD9834172.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1688..1825)
                     /locus_tag="KLF07_004368"
     CDS             complement(1688..1825)
                     /locus_tag="KLF07_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834173.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT          666 a          543 c          322 g          644 t
ORIGIN      
        1 ataaaataca tctattttca aataaaataa agcatactcc aattacaacg agtgactaaa
       61 ataaaaatat catccaagtc aatcactgtt tgctctgcaa aatatgtcag ccttctcaaa
      121 cttaccagca cttaaatatt taagcacaca atcattcctt gagggagttc acttatttca
      181 agagaaacat ttcgtcttcc cacacaacta atccgcccaa tatagctaaa tttgtactca
      241 gtaatatcat ttgttatgcc ccccgccccc aaaatattca agctccttca cagataaaaa
      301 acgacaattt acaaagcctc attagttata actatggaaa tacctatttt agtatatcct
      361 ataaagttag aattacttct tcttgcacct aattccatag ttgaaaaaca agaataattt
      421 tctcctacag ctggatccat atagcttcca tcaggtctac acaacaccca gtggaggccg
      481 acaggaactc caataaagga tacagcaaca gcttccaaaa ctctttggtt agaagataac
      541 acatctcgtt gatgtactat attacatccc atgccaatta ataagtccct tgcatcggga
      601 taaataaaac taattacttg cgaaaaaatg ttactttctt caacgacata agcattcaat
      661 ccaagaagcc tcgtagccat gactatctta tcaggcatag aataaccggc atccgcgata
      721 ttatctttgt gtattctcgg attataatta ccactagtta ttaagtataa atcccgctca
      781 caacgattat ccaaatcaag agaactcttc cttgtcattt cggacataga ccctttgtat
      841 tgtggtattt tatcaacccc taattccttt gcagcacaga gtaaactggc agctccacaa
      901 gaattgtcat acatctgctt cctcgccaaa aaacaacctt gttcgtttac tctattctca
      961 tttaccctca catcagtctg atgagatatc atatattctc ttggtatagc cccctgcaat
     1021 aaaaccccat ataacataac acctgtatga atacatcccc gtttgcaagg cttccgttgt
     1081 tccgacatcg acgagtaatt gcagcacagt attgcatcca gcaatgcgaa ctactatgag
     1141 aattagctga ccgggtcctt attccccgaa cgaactttaa atccaaaacc tcatactctt
     1201 ttccgtcgtc atcacgtccg tcaccataac aacctttcag catccaccct tttattaaga
     1261 gttaatgtcc atcagcatca gcaattaata ccgcctgata ctgacatatt tttgtcgtat
     1321 ttacgtccat aatcggttat tgtgattcag gtcacaccag cttatttgct attgttacaa
     1381 taaccacctt ggttgagagc tgttttttca actttgttat cgactccccc aactccttgg
     1441 tcagtaaggc tgccgaccga gcacagtgaa tagatagtat ccgcaggtac caccatgctt
     1501 acttaatact cagaaggcga agtgctctcc aattaactct gccgcactat gcttataagc
     1561 tgcttttcaa tgccgtctat caacatgtcc agttcattca atctgttgta ctggtaatcc
     1621 aacgcagtaa tgatatatgc aggtattctt tcatccactc cagtaacgtt tcatgcaaag
     1681 cattaattta cgcgctacgg aacttcacca gttgactgag caaacgatgt gggaccagta
     1741 ctgcctgcag ttcttctatt gttacagcga tttctttacc aaacagctga accaccatca
     1801 agataaagat agcctgaaca ttcatcgcac cattttgtta ccaatggcaa aagatttaac
     1861 gaaccgtgcc tgcatcaggc ggacttttta tccctgcttt gttagctccc taaccttgta
     1921 cagagagcct ccataagctc ccatatcaag cagacatggt tcgcagttac tgacatacac
     1981 cagaaaactc tgacagcaga ctcacttatc ctccacttca ctcatatcct tattggtgag
     2041 atgaacctga atgacatgtt ttgcgacata aacgccaacc agtgtatatt tcacctgtag
     2101 agattctaca atctgtagcg ttatgcatct aatgttaggc tctatgatgt cagaaatctg
     2161 cgagactcta cacct
//
LOCUS       DAEEBB010000301         2144 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEEBB010000301 DAEEBB010000000
VERSION     DAEEBB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.307"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..581)
                     /gene="spa40"
                     /locus_tag="KLF07_004369"
                     /gene_synonym="spaS"
     CDS             complement(<1..581)
                     /gene="spa40"
                     /locus_tag="KLF07_004369"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBD9834174.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVI"
     gene            complement(588..1358)
                     /gene="spa29"
                     /locus_tag="KLF07_004370"
                     /gene_synonym="spaR"
     CDS             complement(588..1358)
                     /gene="spa29"
                     /locus_tag="KLF07_004370"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBD9834175.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            complement(1358..1618)
                     /gene="spaQ"
                     /locus_tag="KLF07_004371"
     CDS             complement(1358..1618)
                     /gene="spaQ"
                     /locus_tag="KLF07_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBD9834176.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(1633..>2144)
                     /gene="spa24"
                     /locus_tag="KLF07_004372"
                     /gene_synonym="spaP"
     CDS             complement(1633..>2144)
                     /gene="spa24"
                     /locus_tag="KLF07_004372"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBD9834177.1"
                     /translation="SNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRF
                     SDSGLMEYKQYLKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKD
                     AFKIGFYLYLPFVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKAL
                     IEQYINVPA"
BASE COUNT          869 a          375 c          317 g          583 t
ORIGIN      
        1 ataataacaa aaatagaaaa tgccaagaaa aataatatta tatccttaaa cagccccccc
       61 caaataagat ataagccatc aacactagaa aacacctgag aaaaaattat ttttcggtca
      121 ttaatccaaa agaaataggt tgttaatgct agaataataa gaagcagaat gcttttgaaa
      181 aattctttta ttgtctttat actaaatatt ttttttaacc ctttaacagg gtttaatact
      241 gaaaaatcaa tcttgatagc tttagtcgca agaacaaact ttgtttgaac caatgttggc
      301 aacacagccg aaagaacaca gaaaaaaagt gggaagccaa ttatcttaaa aaagacaata
      361 accactgcaa aaaagtattt accctcatta atttcgaagt cattaattat tacatatctg
      421 tataagagca ttacatcact taaggaaaag aatgatatta tagtaaatgt ccctaccaga
      481 ataataacaa cagtcgttaa atccttaaat ttaaatgact gtcctttttt tgcggcatcc
      541 tttagttttt taggtgtcgg cttttctgtt ttatttgcca taatatttta tctaacaaat
      601 agatttgtga aaaatttatg ttcaccgagg aaaaattgaa ctttagaaaa gtaaatagta
      661 gaagaacaga tgaaaataat aaatattgca agtaaacttt taatagttaa tgatacggaa
      721 aaagcattca tctgcggagc aaatctcgat aatacaccaa gtaatacttc tgatagtaac
      781 aatactatca taacaggact ggctaaaata acagcctgac ttgccaataa agtcagaaat
      841 gttaagatat ttgagacacg gaaagaacat tgagaaaata acgggcatat attataagac
      901 aattgtatgg attctaaaat aaagaccata ccaccactgt atagaaatac aactgcagaa
      961 aaaagattga aaaattttgc caactcagac gtatcaacac cattggcagg atcaattgaa
     1021 ctactaagcg ttgccccacg ctggttgtca ataatgctac caacagcatg aaatatccaa
     1081 aagggaagag acaagcaaaa agagagaagg aggccaacaa ttatttcctt gaacataagg
     1141 aaataaacat gttcaaaaac agaagaacct atgtctacct taccagaagt aattaatcct
     1201 gatgcaacaa gaaaaatcac aggtattcta atagatggag aaattatacc gttatttaaa
     1261 aatggaagaa agaaaaataa tggagccaat ctaaaaaaaa cgccgttcag gagtattaaa
     1321 aacacatgaa tactttcgaa ccagcttgaa atgtccatca aaccccactc ttaattaaaa
     1381 acattatttc atgacaaaaa gacaataaaa cctcaccata ccatccagaa agaagtagca
     1441 aagatattga gacacctata agctttatac caaaaggaag tgtctgctct tggagttgag
     1501 tcactgtctg taacaaacca atagataatc caataaccgt agctatacct actggccata
     1561 aggaaaagat aaggattaaa taaagagcct tattacccat ataaactata tcagacacat
     1621 tacctccatc atctaagcag gaacattgat atattgttca atcaaggcct tagacaggat
     1681 accccaccca tcaagcgcaa caaataatac aagttttatt ggtactgata ttgtgattgg
     1741 actcatcatc atcataccaa gtgcaagtaa aatgcttgaa ataacaagat caacaacaac
     1801 aaatggcaaa tataaataaa aacctatttt gaacgcatct ttgatttcac ttagcgcata
     1861 tgcaggcaag agggaaaaca atgaataatc attattctct gcactcttta aatcagcatt
     1921 ttcttcttca gacctttgga aaaaacgtgc taactcaaga tctgtatgct ttttcaagta
     1981 ttgcttatat tccatcaagc cagaatcaga aaaacggaca atatcagata ttgtatcaaa
     2041 tttttgaggg ccatttagat aattttcata accagcttca ataatgggtt tcatcacgaa
     2101 caaagccata atcaatgcaa taccattgag agtcatattt gatg
//
LOCUS       DAEEBB010000302         2138 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEEBB010000302 DAEEBB010000000
VERSION     DAEEBB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.308"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..1342
                     /gene="espX1"
                     /locus_tag="KLF07_004373"
                     /pseudo
     CDS             1..1342
                     /gene="espX1"
                     /locus_tag="KLF07_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            1576..2138
                     /locus_tag="KLF07_004374"
                     /pseudo
     CDS             1576..2138
                     /locus_tag="KLF07_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          787 a          376 c          336 g          639 t
ORIGIN      
        1 gacaaaatga gttagtaaaa gtattttgcg agtataaaac agagttctta attttgtttg
       61 atgacttttt tagaagccat aacttgccta aaccatctcc agtccttcat catttttttc
      121 aatatactca tctgagagat gcatattttt atagatgtaa actcatagaa catacagttc
      181 aattttattt tttaaacaca aaggtatcac attacttaga ttggatgttt ttgatgacag
      241 aacaagcgaa tgtctttctg aagagattaa gatataccaa gaatgccatg aaaaattcgt
      301 taatttttta aaggcaaact tcaaccaaga aatataccca cagctatata ctcctgaaat
      361 attttatgag gcctgcagaa atctccagtc attttatgat catcaggaaa cgtcacaaaa
      421 cgcaaaatac agtgcaatag acaagaaaaa aagtcatctt aaccaggaaa tcaaaaagct
      481 tattaaaaaa aatattttcc ctgagctata caatgagcaa tgcaataaaa tcccctcctc
      541 atcgatagat gataaccagg gaataatgtg gcagaacttt aaaactagca attcggcata
      601 ttcaaaacta tgtggaaaat tatctttatt taaaagcgtg cccagtcggt taatagaaaa
      661 atcagcttac tgctcaactg aaaacatggc cactaataaa tttaatgtcg tcttttctta
      721 ctgcggagat aatataaagg aatttatact tttattaccc tataataaat cactggagat
      781 gtatgaactt aacgatcaaa agatccagta tttgactgct cttaatatta atatacataa
      841 aatgttgcta tcaaatataa caatagaaaa gagcaacctt tcatatgggt attatttttg
      901 ttgtgtatta accaatatat catgttttga aagcgactta tcaaacacca tcttttcaaa
      961 tggagaaata aacaacctct tcattaaaaa aagcaacata tttggaacat cgttcactaa
     1021 taccatgata aaaaatttgc gctgtgaaga tattatgcct ggccgatgga ccactcaact
     1081 tgtcaataag cacttaggtt acagatacac aggagtattc aaaaccctgg caagtattga
     1141 tgacaaaccg tcccgtttcg aaattttgat accattggta caaactttag ttcgcgataa
     1201 tgtaaagcta aataatgatg tgtataaaga acttaataag tttatgcacg attacgacaa
     1261 aacatcatca aaaatgagaa aataccttca gagcatcaac gagtgcatgc tccttataaa
     1321 aaatatagtt catcaggatt aaaacactca aagaaagagg taattttaga atgaaattat
     1381 ctgtgatgat tattaataca ctgcagagaa ataaaaaaca atctgaagca tgctctgaaa
     1441 catcctactc tgtcactcta atgatggcac cttgctcttc ttataaagaa gggaacaatt
     1501 aagaatatta aattttcaaa ctttcatcaa cttcacatca agaaattaaa aaatttaaac
     1561 attaatctaa ataataacaa cctgatcaca taatcgggta cgattttatc cgataactat
     1621 aatgctataa ctctgggaaa tggttggaat acaccgctgg gtgccatctc tttttacgct
     1681 gcccgatcga gtagtaagtt aaataatgat accagacacg aaggtacgag ctatcaggtc
     1741 gcctataata aatatctagt acaaacagcc actcgattta gcgtggccgc ctggcgttat
     1801 gcttcgcagg attacaggac attcagcgac catctttacg aaaatgataa acactatcat
     1861 cagagtgatt atgatgattt ttatgatatt ggcagaaaaa atagcctttc tgccaatatt
     1921 atgcaacctt tatccaataa tctgggaaat gtatcattaa gtgcgctttg gcggaattac
     1981 tgggggcgaa gtggaaatgc taaagattac caattcagtt actccaataa ctggcaacac
     2041 attagttata ctttctctgc cagccaatct tatgatgaaa ataataaaga agaggagcgt
     2101 tttaatctgt ttatctccat tcctttctac tgggggga
//
LOCUS       DAEEBB010000303         2133 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEEBB010000303 DAEEBB010000000
VERSION     DAEEBB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.309"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..207
                     /locus_tag="KLF07_004375"
     CDS             <1..207
                     /locus_tag="KLF07_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD9834178.1"
                     /translation="QKYEMLENEYSQRVADRLKASGLSGDADAEREAGAQVMRETEQQ
                     IYRQLTDEVLALRLSENGSQLHHS"
     gene            415..684
                     /locus_tag="KLF07_004376"
     CDS             415..684
                     /locus_tag="KLF07_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834179.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            681..907
                     /locus_tag="KLF07_004377"
                     /pseudo
     CDS             681..907
                     /locus_tag="KLF07_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            936..1526
                     /gene="ospG"
                     /locus_tag="KLF07_004378"
     CDS             936..1526
                     /gene="ospG"
                     /locus_tag="KLF07_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBD9834180.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1865..2133
                     /locus_tag="KLF07_004379"
                     /pseudo
     CDS             1865..2133
                     /locus_tag="KLF07_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          601 a          462 c          523 g          547 t
ORIGIN      
        1 cagaagtatg agatgctgga gaatgagtac tctcagaggg tggctgaccg gctgaaagca
       61 tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt gatgcgtgag
      121 actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg attgtctgaa
      181 aacggctcac aactgcacca ttcataatca catcgcataa aactgtcagc gccagtgata
      241 ggacaggtga aacctgtcaa tacgtaccgg cttacgccgg gcttcggcgg ttttactcct
      301 ctgccatatg caacaacgga gtgccgcctt acatgccgct ggcgcggcat atcctggtga
      361 cgatatctga acagttatat actgtgtata tacgtggtaa tgacgaggtg gcaaatggca
      421 caggttaata tgagtgtaag aatcgacgca gaactgaagg atgcttttat ggccgctgca
      481 aaaagcatgg accgtaatgg ctctcagtta atccgggatt ttatgcgcca gaccgttgaa
      541 cggcagcaca attcttggtt ccgtgaccag gttgcggcag gccgtcagca actcgaatgc
      601 ggtgatgtgc ttccccatga catggtcgaa tcctctgctg ctgcatggcg ggatgaaatg
      661 agcaggaaga ttgctgataa atgatggaga tgttctggac catgctggcc atccaggaca
      721 ggaagcgcat tcgtagtaca ttgcggagca gaacctgatg gccgccattg aaccggacga
      781 acggattggt tattcagcca gcagcctggc agggcagccg tataaaggcc gcaacggtcg
      841 tgtggaagga acatctggac cacacaaggt agcgtgtaac gttattttgt gtgaaaatct
      901 cttgtgattt ttaaatatcg taacaaaggt atgacatgaa aataacatct accattattc
      961 aaacaccttt tccatttgag aataataatt ctcatgctgg catagtaacg gagcccattc
     1021 tcggtaagtt aataggtcag gggtcgacag cagaaatctt tgaagatgtg aatgattcat
     1081 ctgctttgta taaaaagtat gatcttattg gcaaccagta caatgagatt ctggaaatgg
     1141 cttggcaaga atctgagctt tttaatgctt tttatggcga tgaagcatcc gttgttatac
     1201 agtatggcgg agatgtgtac ctccgaatgc tgcgcgtgcc tgggactccc cttagtgaca
     1261 ttgatacagc tgatatccct gataatatag agagccttta tctacagttg atatgtaaat
     1321 tgaatgagtt gagtataatc cattacgatc ttaatacagg taatatgctg tatgataaag
     1381 aaagtgaaag tttattccca atagattttc gcaatattta tgctgaatat tacgctgcaa
     1441 ccaaaaaaga taaagagatt atcgaccgac gattacaaat gcgtacaaat gatttttatt
     1501 cgttattaaa caggaaatat ttatagccgt atttgttgat gctataagcc agaggctctt
     1561 ctatgagaat atggggacca ttccctcgcg atattaacgt aatatcttaa ttgtatgcac
     1621 cgccagaatg cgttcagtgc gatcacggca gcagacaggt aaaaatagca acaaaccacc
     1681 cgaaaaaccg ccgcgatcgc gtatgataaa ttttaaccac atgcatacct gtgcagcaag
     1741 gcgaatcacg cacggatttc cagccagaga cacctgaaac gtaaccggct catttaaacc
     1801 gtctggtttg ttacctccgg ttccacaaaa ataatgtcca tcattttgaa gtggacacta
     1861 tcgtatggaa taccggacct gaatcactga agctttacgc cttcatttcg aagaacattt
     1921 accacgggtt gtggccggac gtcgcctggg cgtaccaaaa tcaactgtct gtggtatgtt
     1981 cgtgcgcttc cgcaacgctg gcctttcatg gcctctgccc gcaggtatgt cggagcagga
     2041 acttgatgcc cttctttacg gaagtgcctc cacagtacct gtcgtactta ctgaaagcac
     2101 cgttatgccc aaactcccgg tggtaaaaaa acg
//
LOCUS       DAEEBB010000304         2093 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEEBB010000304 DAEEBB010000000
VERSION     DAEEBB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.310"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..123)
                     /locus_tag="KLF07_004380"
     CDS             complement(<1..123)
                     /locus_tag="KLF07_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBD9834181.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /gene="iraM"
                     /locus_tag="KLF07_004381"
     CDS             384..719
                     /gene="iraM"
                     /locus_tag="KLF07_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBD9834182.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="KLF07_004382"
     CDS             complement(1000..1131)
                     /locus_tag="KLF07_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBD9834183.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="KLF07_004383"
     tRNA            complement(1631..1707)
                     /locus_tag="KLF07_004383"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="KLF07_004384"
     tRNA            complement(1808..1883)
                     /locus_tag="KLF07_004384"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="KLF07_004385"
     CDS             complement(1986..>2093)
                     /locus_tag="KLF07_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD9834184.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          521 c          370 g          525 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgactt ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgagtca ccagcgcatc ata
//
LOCUS       DAEEBB010000305         2089 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEEBB010000305 DAEEBB010000000
VERSION     DAEEBB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.311"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(60..1025)
                     /locus_tag="KLF07_004386"
     CDS             complement(60..1025)
                     /locus_tag="KLF07_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBD9834185.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="KLF07_004387"
     CDS             complement(1879..>2089)
                     /locus_tag="KLF07_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834186.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAEEBB010000306         2088 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEEBB010000306 DAEEBB010000000
VERSION     DAEEBB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.312"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="KLF07_004388"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="KLF07_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HBD9834187.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="KLF07_004389"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="KLF07_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HBD9834188.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAEEBB010000307         2052 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEEBB010000307 DAEEBB010000000
VERSION     DAEEBB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.313"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1161)
                     /locus_tag="KLF07_004390"
     CDS             complement(<1..1161)
                     /locus_tag="KLF07_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBD9834189.1"
                     /translation="MLIYLYPVLLLFNILPVFFYGQMNSDLERFFGVPIGYIPDLIFY
                     FFVVLTSIITLRFHVSLWTKKLLFLGIIFLIYISIQMLLLSADISGVVILLSFFSNFI
                     ALVLLVSFCIGKDELYLTHSVRNINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNAT
                     AIIVVCFYCVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKK
                     EKLLISLFFVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFI
                     GVDVLKENYLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLIS
                     VFYIMLSPIFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIR"
     gene            complement(1230..>2052)
                     /locus_tag="KLF07_004391"
     CDS             complement(1230..>2052)
                     /locus_tag="KLF07_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD9834190.1"
                     /translation="LSLFIEPLYYSAISIFIYSISLLVFSSHKCNVPCFHIKRPSILV
                     YKDFLDATPFAILVLLNVVLSSIDLFILKEYFSYNSVAIYQVVTRVNTGLIIVFNVIY
                     TVLLPSFSYYLKNSEWGNIRKLQRYISLLVLLLCLCYYFFGIYFVGILFGDEYKVISS
                     ATFLIMFMALIKYNFWLINELYLVCSGNQSERVKSYCIGVVISMAVFFYFIPRYGWSG
                     AVFGSAIATLVIGIFYIISVKKDCGKILHDKYSLMMIFVPIFFYFIINGQQRLLY"
BASE COUNT          903 a          366 c          243 g          540 t
ORIGIN      
        1 acgtataaaa accaccgata aaaaaataga aatataaata tatatcgctg gcgtcaatat
       61 atactcatta aaaaagacat agaaagccaa agcaaaaacg catcctttac caatataccc
      121 tccgcattta aaaattggag acagcattat ataaaataca gaaataagca aaataaaacc
      181 aataatccct aactgagccg aataagataa ataaaaatta tgcgccaacc caaagttggt
      241 acttcctaaa aactttttat ctatagcctt tacataattt gcaacaccta atccagtgcc
      301 aattaaataa ttttctttta aaacatcaac cccaataaat actaactcaa ctcgacgata
      361 atcattctga ctttttgtaa gagtgttacc accagagaat ataacctgtg acgaagacag
      421 cattcgttca agacgagtgc ctatatcagt aaaagaaata cctaaagtta gaagaggaac
      481 aaaaaacaat gatattaata acttttcctt ctttgttaac tgaagaaaaa caaaaaacaa
      541 cgatatagca aatgatatta ttcctgctcg gctatccaga aaaagaaaga acagagagta
      601 caatacagat gagacatacc aagactttcg accacgataa aagtatgaat atacacaata
      661 aaagcacact actataattg cggtggcatt tctatttaaa taaactataa aattactatc
      721 ttcagaataa ccaataaata attttacaac tccacagata ataccaaaac acattacaac
      781 atttatattt ctaaccgaat gagttaaata aagctcatct ttaccaatgc aaaatgacac
      841 caaaagaacc aaagctataa aattagaaaa aaacgataat aaaattacga cacctgatat
      901 atccgctgat aacaacaaca tctgaatgct gatataaatc aggaatatga tgcctaaaaa
      961 taataatttc tttgtccaca gagaaacgtg aaacctcaac gttattatag atgttaaaac
     1021 aacaaagaaa taaaatatta gatctggaat atagccaata ggaactccaa aaaaacgctc
     1081 taaatcagag ttcatttgtc cataaaaaaa aaccggaagg atattaaata acaaaagtac
     1141 aggatatagg tatattaaca tacttaacct ttattaggct tccaatcgag tctaaacata
     1201 ttaatggaac gatataaaac cacaacatat taatataaca accgctgctg accattaata
     1261 ataaaataaa agaaaattgg gacaaagatc atcattagtg aatacttatc atgaagaatt
     1321 ttcccacaat cttttttcac agaaataata taaaatattc caattactaa tgttgcaatg
     1381 gcacttccaa aaaccgcccc actccatcca taccgaggta taaaataaaa gaaaaccgcc
     1441 attgaaatga ccacaccaat acaatacgat ttaactcgct cgctttgatt tccactacac
     1501 acaagataaa gttcatttat tagccaaaaa ttatatttaa taagagccat aaacattatc
     1561 aaaaatgttg cagaagatat taccttatac tcatcaccaa acaatatccc tacgaaatag
     1621 atgccaaaaa aataatagca taaacatagt aataagacta acagtgatat atatcgttgt
     1681 aatttcctta tattacccca ttcagaattt ttcagataat aagaaaatga aggcaataaa
     1741 acagtataaa taacattaaa cactattatt agaccggtat taaccctagt taccacctga
     1801 tatatagcaa cactattata agagaaatat tcttttaata taaaaaggtc aatactagat
     1861 aaaacaacat ttagtaacac cagaatagcg aacggagttg catccaaaaa atctttataa
     1921 actaaaatac ttggtctttt tatatgaaaa catggcacat tgcatttatg tgatgagaaa
     1981 accaaaagcg aaattgagta tatgaatatt gatatcgcac tataatatag aggttcgata
     2041 aacagagata aa
//
LOCUS       DAEEBB010000308         2013 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEEBB010000308 DAEEBB010000000
VERSION     DAEEBB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.314"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(37..273)
                     /locus_tag="KLF07_004392"
     CDS             complement(37..273)
                     /locus_tag="KLF07_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBD9834191.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="KLF07_004393"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="KLF07_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBD9834192.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="KLF07_004394"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="KLF07_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBD9834193.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..>2013)
                     /locus_tag="KLF07_004395"
     CDS             complement(1875..>2013)
                     /locus_tag="KLF07_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBD9834194.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          527 a          530 c          439 g          517 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAEEBB010000309         2007 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEEBB010000309 DAEEBB010000000
VERSION     DAEEBB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.315"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..95
                     /locus_tag="KLF07_004396"
                     /pseudo
     CDS             1..95
                     /locus_tag="KLF07_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            204..472
                     /locus_tag="KLF07_004397"
                     /pseudo
     CDS             204..472
                     /locus_tag="KLF07_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            862..>2007
                     /locus_tag="KLF07_004398"
     CDS             862..>2007
                     /locus_tag="KLF07_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD9834195.1"
                     /translation="MIKSTNIQAIGSSIMHQINNIYSLTPFSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTS
                     GFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKT"
BASE COUNT          677 a          427 c          342 g          561 t
ORIGIN      
        1 aagcggctgg cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca
       61 tcggctcctt cattgaaaaa cacatattct actgattgga agcgccaccc gaaaaagtat
      121 cgttttttta cagtaaactt catttagccg actataatgt aaaaaaataa attttttacc
      181 aaaatataac ataaggaagt aatatgctta cacaaactat attcccttgt cttcccaaaa
      241 aataagaaaa tatcatccct gaagtatcaa atactatatt attatcttct actgttacta
      301 cagatggata tactgttttt aataaaaaaa aacagccata tacgaattgc aaatacctgc
      361 taccaaccga acgaaaaggt tgaaattcac agctacagaa atacaatggt tgacaaagat
      421 aaatgaagcc ggaattgata aaaaacaaag ccaacggtat tctgatttct gaagttaatt
      481 tcagactcgt ttaagctcta ttttcaaaaa aatatatcag ggtttgttac taataacttc
      541 tgaaaacaga aaaaaataga gcagtaattc tatttcagag aggagtaact ccatctatcc
      601 tcgatacgta ctgaacgtta tcgtgatata ttcatcatgc tttcttcacc attattcgag
      661 tacagggaga gacgcaatga ccttaaatac ctttttatgg ctgaattacc attctcagga
      721 ataacagcag actccttccc atagacggct tagggggaaa gcaatacccg gagaaagggt
      781 acaaagcaaa ctgttttaaa attaataagc tatattctat ttcactagaa ttcttatcat
      841 tgataagaaa ttttagttat aatgattaaa tcaaccaata tacaggcaat cggttctagt
      901 attatgcatc aaataaacaa tatatactcg ttaactccat tttctttacc tatggaactg
      961 actccatctt gtaatgaatt ttatttaaaa gcctggagtg aatgggaaaa gaacggtacc
     1021 ccaggcgagc aacgcaatat cgccttcaat aggctgaaaa tatgtttaca aaatcaagag
     1081 gcagaattaa atttatctga gttagattta aaaacattac cagatttacc gcctcagata
     1141 acaacactgg aaataagaaa aaacctatta acacatctcc ctgatttacc accaatgctt
     1201 aaggtaatac atgctcaatt taatcaactg gaaagcttac ctgccttacc cgagacgtta
     1261 gaagagctta atgcgggtga taacaagata aaagaattac catttcttcc tgaaaatcta
     1321 actcatttac gggttcataa taaccgattg catattctgc cactattgcc accggaacta
     1381 aaattactgg tagtttctgg aaacagatta gacagcattc ccccctttcc agataagctt
     1441 gaagggctgg ctctggctaa taattttata gaacaactac cggaattacc ttttagtatg
     1501 aacagggctg tgctaatgaa taataatctg acaacacttc cggaaagtgt cctgagatta
     1561 gctcagaatg ccttcgtaaa tgttgcaggt aatccattgt ctggccatac catgcgtaca
     1621 ctacaacaaa taaccaccgg accagattat tctggtcctc gaatattttt ctctatggga
     1681 aattctgcca caatttccgc tccagaacac tccctggctg atgccgtgac agcatggttc
     1741 ccggaaaaca aacaatctga tgtatcacag atatggcatg cttttgaaca tgaagagcat
     1801 gccaacacct tttccgcgtt ccttgaccgc ctttccgata ccgtctctgc acgcaatacc
     1861 tccggattcc gtgaacaggt cgctgcatgg ctggaaaaac tcagtgcctc tgcggagctt
     1921 cgacagcagt ctttcgctgt tgctgctgat gccactgaga gctgtgagga ccgtgtcgcg
     1981 ctcacatgga acaatctccg gaaaacc
//
LOCUS       DAEEBB010000310         2005 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEEBB010000310 DAEEBB010000000
VERSION     DAEEBB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.316"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..389
                     /locus_tag="KLF07_004399"
     CDS             <1..389
                     /locus_tag="KLF07_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834196.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="KLF07_004400"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="KLF07_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBD9834197.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="KLF07_004401"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="KLF07_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBD9834198.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="KLF07_004402"
     tRNA            complement(1806..1881)
                     /locus_tag="KLF07_004402"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="KLF07_004403"
     tRNA            complement(1890..1966)
                     /locus_tag="KLF07_004403"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAEEBB010000311         1996 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEEBB010000311 DAEEBB010000000
VERSION     DAEEBB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.317"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..689
                     /locus_tag="KLF07_004404"
     CDS             <1..689
                     /locus_tag="KLF07_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD9834199.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
     gene            853..1335
                     /locus_tag="KLF07_004405"
                     /pseudo
     CDS             853..1335
                     /locus_tag="KLF07_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1450..>1996
                     /locus_tag="KLF07_004406"
     CDS             1450..>1996
                     /locus_tag="KLF07_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD9834200.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          668 a          412 c          344 g          572 t
ORIGIN      
        1 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
       61 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg
      121 aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt
      181 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      241 ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga
      301 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      361 attacgctga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      421 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      481 acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata
      541 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
      601 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg
      661 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
      721 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat
      781 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
      841 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
      901 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
      961 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1021 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1081 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1141 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa
     1201 attaacacga taacctttcc aaaagaatca aaactggaaa acatttatat tgatcataac
     1261 aatctggaaa gtttagattt aaaaaatcag cattcattgg ttaatctgga agcgcaaaat
     1321 aacaaactga caaaaattaa tatttctgat agttataaac tgaaatttct taatcttgat
     1381 tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga gttaagtgcc
     1441 catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact
     1501 ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact ggaatattta
     1561 aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa
     1621 catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca aatgcagtac
     1681 tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga
     1741 cttaatttaa ccactcaacc accagggctg caaaacttca catctatcaa tcttgataat
     1801 aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat
     1861 agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat tacacatata
     1921 tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc agttacttat
     1981 tttagtgcgg cacata
//
LOCUS       DAEEBB010000312         1937 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEEBB010000312 DAEEBB010000000
VERSION     DAEEBB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.318"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            73..1461
                     /locus_tag="KLF07_004407"
     CDS             73..1461
                     /locus_tag="KLF07_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD9834201.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1504..>1937
                     /gene="yeaK"
                     /locus_tag="KLF07_004408"
     CDS             1504..>1937
                     /gene="yeaK"
                     /locus_tag="KLF07_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBD9834202.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          509 a          451 c          456 g          521 t
ORIGIN      
        1 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
       61 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
      121 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
      181 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
      241 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
      301 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
      361 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
      421 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
      481 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
      541 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
      601 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
      661 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
      721 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
      781 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
      841 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
      901 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
      961 cgaatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     1021 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     1081 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     1141 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     1201 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     1261 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     1321 caacatatcg cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa
     1381 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     1441 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     1501 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     1561 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     1621 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     1681 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     1741 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
     1801 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     1861 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
     1921 atgctggata aatccgt
//
LOCUS       DAEEBB010000313         1899 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEEBB010000313 DAEEBB010000000
VERSION     DAEEBB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.319"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(89..643)
                     /locus_tag="KLF07_004409"
     CDS             complement(89..643)
                     /locus_tag="KLF07_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD9834203.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="KLF07_004410"
     CDS             complement(640..930)
                     /locus_tag="KLF07_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD9834204.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="KLF07_004411"
     CDS             complement(930..1529)
                     /locus_tag="KLF07_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD9834205.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="KLF07_004412"
     CDS             complement(1601..1813)
                     /locus_tag="KLF07_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834206.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAEEBB010000314         1884 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEEBB010000314 DAEEBB010000000
VERSION     DAEEBB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.320"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            76..345
                     /locus_tag="KLF07_004413"
                     /pseudo
     CDS             76..345
                     /locus_tag="KLF07_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            460..630
                     /locus_tag="KLF07_004414"
     CDS             460..630
                     /locus_tag="KLF07_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834207.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(757..1314)
                     /locus_tag="KLF07_004415"
     CDS             complement(757..1314)
                     /locus_tag="KLF07_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD9834208.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1311..1586)
                     /locus_tag="KLF07_004416"
     CDS             complement(1311..1586)
                     /locus_tag="KLF07_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBD9834209.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1771..1884
                     /locus_tag="KLF07_004417"
                     /pseudo
     CDS             1771..1884
                     /locus_tag="KLF07_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          528 a          387 c          457 g          512 t
ORIGIN      
        1 ttttcattga tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcgatga
       61 attacaaggg gttaaatgct gccgcagcat agcgatattg aaatagccta gtatgcttcg
      121 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
      181 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      241 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      301 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      361 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      421 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      481 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      541 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      601 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      661 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      721 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      781 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      841 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      901 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      961 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
     1021 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
     1081 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1141 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1201 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1261 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1321 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1381 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1441 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1501 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1561 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1621 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1681 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1741 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1801 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1861 tgggtgttta tgttctcagc agga
//
LOCUS       DAEEBB010000315         1870 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEEBB010000315 DAEEBB010000000
VERSION     DAEEBB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.321"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..699)
                     /gene="ipaH9.8"
                     /locus_tag="KLF07_004418"
     CDS             complement(<1..699)
                     /gene="ipaH9.8"
                     /locus_tag="KLF07_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBD9834210.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDY"
     gene            complement(1442..1870)
                     /locus_tag="KLF07_004419"
                     /pseudo
     CDS             complement(1442..1870)
                     /locus_tag="KLF07_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          494 a          399 c          450 g          527 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt
       61 atcactaata tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact
      121 tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc
      181 tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga
      241 tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc
      301 tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac
      361 gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc
      421 aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt
      481 gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag
      541 acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg cttgtttttc
      601 ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt tataaaaaga
      661 attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc agttaaagcc
      721 ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg
      781 aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct gtaaaaaaac
      841 gatactgttt cagttaaccg tgaatggcct gtttgcaccg tatgtgacag taatacgctg
      901 cttcatgata agccctcaaa aacatactta tgatatgtat atgagtatat attaatggta
      961 caaaccatca atatccataa aaaatacacc attaattcgc tataagtttt taatacaatt
     1021 ttttctgctg cgattttttt atacgataat tagcatactt ataacaccgt aagtaaatta
     1081 catgaaagca ggcacagcgt gatccgattt ttctgcgtat aacgattttc cggaagtgta
     1141 atacggttgc gccccgggcc gggccccctt ttctgttgtt gtgtacgggc ccacagagaa
     1201 tggtaccgga ggcgggctca gaagtactga gtcgggtgat cgcgtcaggc cgcgaagtac
     1261 tgacacgggt attggtgact ggtgatgaag tactgccccg ggtatgattt caggctgatt
     1321 caggttctga ttctctgtca gtccgcgtgg ttgctggcct ggctgatttc ctgttgtcag
     1381 gcgttcaaca accgactgct ggttatagcc cggttcacga acacctgacg gcaggtatca
     1441 ctcacccggt gaaggtaaat ccctcaagag gcagcaactc tgccagtcgc tcctccggcc
     1501 attccggcag acgcgtcagg acgtccgtca gccaggcatg tggctccaga ccattacgtt
     1561 ttgctgttta cagaaggctc attatctgtg ccgcccggtt tccggccatc agtgaaccgg
     1621 cgaacagcca cgctttccgt cccataacaa ccgggcgaat ggcacgctcg cagatattgt
     1681 tgtccagggg caccgctccg tcacccagga agcagctcag ttcatcgcgc cggttcagaa
     1741 tatagttaat cgccttattt aatggcccgt cgggcggaca gcacggctcc tgctcttcaa
     1801 gccaggcaaa caggtcattc actatcggcc gggagtaccg ttatcgccac tgccgtattt
     1861 tttccacagg
//
LOCUS       DAEEBB010000316         1823 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEEBB010000316 DAEEBB010000000
VERSION     DAEEBB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.322"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(50..193)
                     /locus_tag="KLF07_004420"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="KLF07_004420"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..916)
                     /locus_tag="KLF07_004421"
     CDS             complement(197..916)
                     /locus_tag="KLF07_004421"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9834211.1"
                     /translation="MTIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1057..1332
                     /locus_tag="KLF07_004422"
     CDS             1057..1332
                     /locus_tag="KLF07_004422"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9834212.1"
                     /translation="MVLESQGEYDSQWAVICSITPKIGCTPETLRVWVRQHERDTRGG
                     DGGLTTAERQRLKELERENRELRCSNDILRQASAYFAKAEFDRLWKK"
     misc_feature    1287..1403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1332..1481
                     /locus_tag="KLF07_004423"
     CDS             1332..1481
                     /locus_tag="KLF07_004423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834213.1"
                     /translation="MPLLDKLRGQYGVGPVCSELHIAPSTYYHCQQQDIILINAVPVR
                     STMTG"
     gene            1418..1816
                     /locus_tag="KLF07_004424"
     CDS             1418..1816
                     /locus_tag="KLF07_004424"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9834214.1"
                     /translation="MSATGHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQ
                     LLREGIRVARCTVARLMAVMGLVGVLRGKKVRTTVSRKTVAAGDRVNRQFVAERPDQL
                     WVADFTYVSTWQGFVYVAFIIDVFAGYIVW"
BASE COUNT          458 a          403 c          436 g          526 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tct
//
LOCUS       DAEEBB010000317         1818 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEEBB010000317 DAEEBB010000000
VERSION     DAEEBB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.323"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="KLF07_004425"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="KLF07_004425"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBD9834215.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="KLF07_004426"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="KLF07_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1818)
                     /locus_tag="KLF07_004427"
     CDS             complement(1450..>1818)
                     /locus_tag="KLF07_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD9834216.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAEEBB010000318         1807 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEEBB010000318 DAEEBB010000000
VERSION     DAEEBB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.324"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1138
                     /locus_tag="KLF07_004428"
     CDS             <1..1138
                     /locus_tag="KLF07_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD9834217.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="KLF07_004429"
     CDS             1150..1599
                     /locus_tag="KLF07_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834218.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          547 a          298 c          505 g          457 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattaccta
     1801 aagctgt
//
LOCUS       DAEEBB010000319         1803 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEEBB010000319 DAEEBB010000000
VERSION     DAEEBB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.325"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="KLF07_004430"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="KLF07_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBD9834219.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="KLF07_004431"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="KLF07_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBD9834220.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..1538)
                     /locus_tag="KLF07_004432"
                     /pseudo
     CDS             complement(1251..1538)
                     /locus_tag="KLF07_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
BASE COUNT          417 a          440 c          443 g          503 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tgg
//
LOCUS       DAEEBB010000320         1800 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEEBB010000320 DAEEBB010000000
VERSION     DAEEBB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.326"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..859)
                     /locus_tag="KLF07_004433"
     CDS             complement(<1..859)
                     /locus_tag="KLF07_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD9834221.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAI"
     gene            1236..>1800
                     /gene="ypdA"
                     /locus_tag="KLF07_004434"
     CDS             1236..>1800
                     /gene="ypdA"
                     /locus_tag="KLF07_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBD9834222.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT          413 a          486 c          439 g          462 t
ORIGIN      
        1 caatcgccgc cacctgcaac ggcgtaaaag tgccgtaatc gtgatagctt ttgattcgcg
       61 ccagggcact gaccagcgtt ttgttgccga ccataaagcc gatacgccag cccgccatgt
      121 tgtagctttt cgacagcgta aagaactcga ccgccacatc gcgtgcaccc ggcacctgca
      181 taattgacgg cgctttccag ccatcgtaga cgatatcggc ataggccagg tcatggacca
      241 ccagcacatc gtagcgtttc gccagcgcca caaccttttc gaagaactcc agttccacgc
      301 attgcgcggt tgggttcgac gggaagccga ggatcatcat cttcggtttc ggataacttt
      361 cacgaatggc gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc
      421 gtacctgcgc cccggcaatc accgcgccat aaatatggat cgggtaactt ggattcggca
      481 ccagcaccgt gtcaccatga tccagcgtcg ccagcatcag atgcgccagg ccctctttcg
      541 aaccaatagt gacgatggct tctgattccg ggtcgatttc aacgtcgtag cgatcctgat
      601 accagcggga aatggcgcga cgtaaccgcg gaatgccgcg tgaagtggag taaccatgcg
      661 tgtccgggcg ctgggccaca gtacataatt tttcgacgat atgcggcgga gtcgcaccgt
      721 ccgggttacc catgctgaaa tcgataatat cttcgccgcg ccgacgcgca gccattttca
      781 gttcagcggt gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt
      841 cagggcgagt gtcagccata atttcctcag attaacgtta gcgcccggac cgtccgagcg
      901 acgctgccac aatggtggct gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt
      961 tttagaacat ttttatccat ggctttaaat gaggaactcc acttcaaaaa caggaaattt
     1021 ttctacgcta acaagaaaat taccaccaat tttattaaca ttataatttc aataaattta
     1081 ccagccagaa gtgcattatc aagagataca ttgctaatac accatttatc tttcaatccc
     1141 cttttaaatt tatggttttt cctcaaaaac ggcctgctgg tcatcagcct tcaggttacc
     1201 tatcatagag gtttaatcct tattcagagt cacccgtgca cgaaatattc aacatgctgc
     1261 tggcggtctt cgatcgggca gcgttaatgc ttatctgcct gttctttctc atccgtatcc
     1321 gcctgtttcg cgaactgttg cacaagtcgg cgcactcccc aaaagaattg ctcgccgtta
     1381 ccgccatttt ctcgctgttc gccctgttca gcacctggtc cggcgttccc gtagaaggct
     1441 cgctggtgaa cgtacgtatt atcgcggtga tgtccgacgg gattctgttt ggcccgtggg
     1501 taggcatcat taccggcgtg attgcgggta ttcaccggta tttaattgat atcggcggcg
     1561 tgacggcgat cccctgcttt atcaccagca ttctggcggg ttgtatatca ggctggataa
     1621 acctgaaaat ccccaaagca cagcgctggc gcgtcagtat tctcggcggc atgttgtgtg
     1681 agacgttgac catgattctg gtaattgtct gggcaccaac taccgcgttg gggatcgata
     1741 tcgtctctaa aatcggcatt ccaatgatcc tcggtagcgt ctgtatcggc tttattgtgc
//
LOCUS       DAEEBB010000321         1793 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEEBB010000321 DAEEBB010000000
VERSION     DAEEBB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.327"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            163..1254
                     /gene="ipaC"
                     /locus_tag="KLF07_004435"
     CDS             163..1254
                     /gene="ipaC"
                     /locus_tag="KLF07_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBD9834223.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            1305..>1793
                     /gene="ipaD"
                     /locus_tag="KLF07_004436"
     CDS             1305..>1793
                     /gene="ipaD"
                     /locus_tag="KLF07_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBD9834224.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQM"
BASE COUNT          666 a          382 c          289 g          456 t
ORIGIN      
        1 aactgtcaaa atatatcagt gaagcaatag aaaaattcgg ccaattgcag gaagtaattg
       61 cagatctatt agcctcaatg tccaactctc aggctaatag aactgatgtt gcaaaagcaa
      121 ttttgcaaca aactactgct tgatacaaat aaggagaatg ttatggaaat tcaaaacaca
      181 aaatcaaccc agattttata tacagatata tccacaaaac aaactcaaag ttcttccgaa
      241 acacaaaaat cacaaaatta tcagcagatt gcagcgcata ttccacttaa tgtcggtaaa
      301 aatcccgtat taacaaccac attaaatgat gatcaacttt taaagttatc agagcaggtt
      361 cagcatgatt cagaaatcat tgctcgcctt actgacaaaa agatgaaaga tctttcagag
      421 atgagtcaca cccttactcc agagaacact ctggatattt ccagtctttc ttctaatgct
      481 gtttctttaa ttattagtgt agccgttcta ctttctgctc tccgcactgc agaaactaaa
      541 ttgggctctc aattgtcatt gattgcgttc gatgctacaa aatcagctgc agagaacatt
      601 gttcggcaag gcctggcagc cctatcatca agcattactg gagcagtcac acaagtaggt
      661 ataacgggta tcggtgccaa aaaaacgcat tcagggatta gcgaccaaaa aggagcctta
      721 agaaagaacc ttgccactgc tcaatctctt gaaaaagagc ttgcaggttc taaattaggg
      781 ttaaataaac aaatagatac aaatatcacc tcaccacaaa ctaactctag cacaaaattt
      841 ttaggtaaaa ataaactggc gccagataat atatccctgt caactgaaca taaaacttct
      901 cttagttctc ccgatatttc tttgcaggat aaaattgaca cccagagaag aacttacgag
      961 ctcaataccc tttctgcgca gcaaaaacaa aatattggcc gtgcaacaat ggacacatca
     1021 gccgttgctg gtaatatatc cacatcagga gggcgttatg catctgctct tgaagaagaa
     1081 gaacaactaa tcagtcaggc cagcagtaaa caagcagagg aagcatccca agtatctaaa
     1141 gaagcatccc aagcgacaaa tcaattaata caaaaattat tgaatataat tgacagcatc
     1201 acccaatcaa ggaattcgac agccagtcag attgctggta acattcgagc ttaatatatc
     1261 caagagccat aataatatat ggctcttcct gtaaggaaat aaccatgaat ataacaactc
     1321 tgactaatag tatttccacc tcatcattca gtccaaacaa taccaacggt tcatcaaccg
     1381 aaacagttaa ttctgatata aaaacaacga ccagttctca tcctgtaagt tcccttacta
     1441 tgctcaacga cacccttcat aatatcagaa caacaaatca ggcattaaag aaagagcttt
     1501 cacaaaaaac gttgactaaa acatcgctag aagaaatagc attacattca tctcagatta
     1561 gcatggatgt aaataaatcc gctcaactat tggatattct ttccaggaac gaatatccaa
     1621 ttaataaaga cgcaagagaa ttattacatt cagccccgaa agaagccgag cttgatggag
     1681 atcaaatgat atctcataga gaactgtggg ctaaaattgc aaactccatc aatgatatta
     1741 atgaacagta tctgaaagta tatgaacatg ccgttagttc atatactcaa atg
//
LOCUS       DAEEBB010000322         1728 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEEBB010000322 DAEEBB010000000
VERSION     DAEEBB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.328"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..663)
                     /locus_tag="KLF07_004437"
                     /pseudo
     CDS             complement(1..663)
                     /locus_tag="KLF07_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(803..>1728)
                     /locus_tag="KLF07_004438"
     CDS             complement(803..>1728)
                     /locus_tag="KLF07_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBD9834225.1"
                     /translation="DTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGKTLRH
                     SYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDA
                     MGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHG
                     REEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSAD
                     LTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTND
                     KVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          464 a          391 c          304 g          569 t
ORIGIN      
        1 tgtcattctc tggattattt aattctttca gcggaaattt ttcctcaaag gctttattta
       61 gccgcccctg gtttcctttt accgcgaata aataatcacc tccctgtttt tgtatcttct
      121 ctgcaatatc tttctggcaa cccatcgcat cagttgtgat gatttttcct ttaatatcca
      181 gcatgttaag aagttcagga atggctgtaa tctcattgga tttctcatcc gtcttaatct
      241 gcccgagaat cagactgtgc attgttgaga acgcactaat gacatgaatt gctcccctgc
      301 ggcactcttg tcataagagt gccggagcgt ttttccatca attgcaatga cgtctttatc
      361 atctgaagaa tggcagtcac gcatccagtt aataaagcac tcgtgaaatt ttgcaggact
      421 gatacaggat acaactctgg cgatggtatc gtgaacagga ataccatttt caaaatcacc
      481 atattgtttt aaaaaatcga gatgtgtttc cccaaaatcc tctatatctt cccagccttc
      541 tgcaccagaa ataacggcac aaatagtcaa cagtagaata tccgataatt tatgttccac
      601 tttccaggct tgtttttaat cggggataat agaaatatgt tccatcaatt ttttaagttc
      661 cattttgttc tccttaatta tgtaaggagt atttgatcat gtataagcaa taaaaaacag
      721 cttcaggtaa taaggaatat ctcaattttt aaacataaaa tgcgaattat ttagtacaaa
      781 aagtgaactc ctcagggcaa gattacgaaa gtccgctccc cgcaaggact gacgcgagat
      841 agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat
      901 cattcgtcaa aacattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc
      961 ctcttcttat tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagattat
     1021 tctccacgtg ccagtgattt cgaatcgctg tcgcaaactt ctcagcggtt aaatcagcag
     1081 aactgatata atatctgacc atcatttcgg gctctttctt ttgctctgct attattgagc
     1141 gaaaggagac tgccacgcat aattttttca gccctttcca ttcaaacgtg aaatcaataa
     1201 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
     1261 cactcattgc ggaactgtca tgcgctggat tatttaattc tgtcagcgga aattttttct
     1321 caaaggcttt atttagccgc ccctggtttc cttttaccgc aaataaataa tcacctccct
     1381 gtttttgtat cttctctgca atatctttct ggcaacccat cgcatcagtt gtgatgattt
     1441 ttcctttaat atccagcatg ttaagaagtt ctgggatagc tgtaatctca ttagatttct
     1501 catccgtctt gatctgtccg atgaccagac tgtgcattgt tgagaacgca ctaatgacat
     1561 gaatcgctcc cctgcggcga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg
     1621 caatgacgtc tttatcatct gtagaatggc agtcacgcat ccagttaata aagcactcgt
     1681 gaaattttgc aggactgata caggatacaa ctctggcaat ggtatcgt
//
LOCUS       DAEEBB010000323         1698 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEEBB010000323 DAEEBB010000000
VERSION     DAEEBB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.329"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..177
                     /locus_tag="KLF07_004439"
     CDS             <1..177
                     /locus_tag="KLF07_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD9834226.1"
                     /translation="STIVVSQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQGESL
                     RKNPPTVESSEKTS"
     gene            266..1698
                     /gene="rhsC"
                     /locus_tag="KLF07_004440"
                     /pseudo
     CDS             266..1698
                     /gene="rhsC"
                     /locus_tag="KLF07_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          430 a          482 c          511 g          275 t
ORIGIN      
        1 agcacgatag tggtaagcca gttcccggta gataaatggc atggtctgat ggagaaccca
       61 acaacagcag atgccatcct ggacagactg gtacataact cacacagagt ggtccttcag
      121 ggagagtccc tgaggaaaaa tcccccgaca gtggaaagca gcgaaaaaac gagttaaaaa
      181 gcggtagaaa aagttaagtt cccggttgat cgaatggatg cgaatcgctg atcgaacttc
      241 gatgccaatc cgtgatcgaa tagagcggaa tacgcagctg gtactgacca cgcaggcgca
      301 gcgggcagaa gaagcccggc agcaggccat ttccggcggg acggaaccgt ccgcttttcc
      361 tgataccctg ccgggttaca ccgaatatgg ccgggacaac ggcatccgtc tgtctgccgt
      421 gtggctgacg cacgacccgg aatacccgga gaatttacct gccgcgccgc tggtgcgcta
      481 tggctggacg ccgcgcggcg aactggcggc agtgtatgac cgcagcaata cacaggtgcg
      541 cagctttact tacgatgata aataccgggg ccggatggtg gcgcaccgtc acacgggccg
      601 gccggaaatc cgttaccgtt acgacagcga cgggcgggtg acagaacagc taaacccggc
      661 aggcttaagc tacacgtatc agtatgagaa ggaccgcatc accatcaccg acagcctgaa
      721 ccgccgtgaa gtgctgcaca cgcagggtga agccgggctg aagcgggtgg tgaaaaagga
      781 acacgcggac ggcagcgtca cgcagagtca gtttgatgcg gtgggcaggc tcagggcaca
      841 gacggatgcc gcaggccgga cgactgagta cagcccggat gtggtgacgg gactcatcac
      901 gcgcatcacc acgccggatg gcagggcatc ggcgttttac tataaccacc acagccagtt
      961 aacgtcagcc accgggcctg acgggctgga aatgcgccgc aaatatgatg aatccggccg
     1021 tctgattcag gaaactgccc ctgacggcga tatcatccgc taccgttatg ataatccaca
     1081 cagtgactta ccctgcgcaa cggaagatgc caccggcagc cggaaaacca tgacgtggag
     1141 ccgttacggt cagttgctga gcttcaccga ctgttccggt tatgtaaccc gttatgacca
     1201 tgaccgcttc gggcagatga cggcggtgca ccgcgaggaa gggctgagtc agtaccgcgc
     1261 atacgacagc cgtggacagt taattgccgt gaaagacacg cagggccatg aaacgcggta
     1321 tgaatacaac gccgccggtg acctgaccac cgtcattgcc ccggacggca gcagaaacgg
     1381 gacacagtac gatgcgtggg gaaaagctat ctgtaccacg cagggcggtc tgacgcgcag
     1441 tatggaatac gatgctgccg gacgggtcat ccgcctgacc agtgaaaacg gcagccacac
     1501 caccttccgt tacgatgtac tcgacctgct gatacaggaa accggctttg acggccgcac
     1561 acagcgttat caccacgacc tgaccggcaa acttatccgc agcgaggatg aggggctggt
     1621 cacccactgg cactatgacg aagcagaccg cctcacgcac cgcaccgtga atggcgaaac
     1681 cgcagagcgg tggcagta
//
LOCUS       DAEEBB010000324         1686 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEEBB010000324 DAEEBB010000000
VERSION     DAEEBB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.330"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="KLF07_004441"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="KLF07_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBD9834227.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1686
                     /locus_tag="KLF07_004442"
     CDS             1594..>1686
                     /locus_tag="KLF07_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834228.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          343 c          301 g          491 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataac
//
LOCUS       DAEEBB010000325         1676 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEEBB010000325 DAEEBB010000000
VERSION     DAEEBB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.331"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..889)
                     /locus_tag="KLF07_004443"
     CDS             complement(<1..889)
                     /locus_tag="KLF07_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9834229.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="KLF07_004444"
     CDS             complement(989..1345)
                     /locus_tag="KLF07_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBD9834230.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="KLF07_004445"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KLF07_004445"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAEEBB010000326         1667 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEEBB010000326 DAEEBB010000000
VERSION     DAEEBB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.332"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(183..604)
                     /locus_tag="KLF07_004446"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="KLF07_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(588..815)
                     /locus_tag="KLF07_004447"
     CDS             complement(588..815)
                     /locus_tag="KLF07_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD9834231.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="KLF07_004448"
     CDS             892..1299
                     /locus_tag="KLF07_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD9834232.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="KLF07_004449"
     CDS             1503..1658
                     /locus_tag="KLF07_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBD9834233.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAEEBB010000327         1643 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEEBB010000327 DAEEBB010000000
VERSION     DAEEBB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.333"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..451
                     /gene="icsB"
                     /locus_tag="KLF07_004450"
     CDS             <1..451
                     /gene="icsB"
                     /locus_tag="KLF07_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBD9834234.1"
                     /translation="QRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIH
                     TPKTFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDK
                     KEEIIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            464..853
                     /gene="ipgA"
                     /locus_tag="KLF07_004451"
     CDS             464..853
                     /gene="ipgA"
                     /locus_tag="KLF07_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBD9834235.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            869..1495
                     /gene="ipgB1"
                     /locus_tag="KLF07_004452"
     CDS             869..1495
                     /gene="ipgB1"
                     /locus_tag="KLF07_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBD9834236.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSNMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            1553..>1643
                     /locus_tag="KLF07_004453"
     CDS             1553..>1643
                     /locus_tag="KLF07_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CesD/SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HBD9834237.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINA"
BASE COUNT          624 a          297 c          232 g          490 t
ORIGIN      
        1 gcaacgcatt gaaagtctca atcagtctta taacgagata atgaagtata ttgaatctga
       61 ttttgatctt tccagattga ctcaattacg acgtagctat cttaaaagtt tcaataaaat
      121 taaccttatc cacaccccaa aaacattcaa gcctttatca atttcacttt acaaacatcc
      181 tactgaaaat gtatcttcag aagactttga tgcggtcatc aacgcttgcc actcatactt
      241 agtaaaatca gcacccagca atatgtctcg tgtattaaac gaattaaaaa ccggagcaac
      301 agataaaaaa gaagaaatta tcgaaaagtc aataaaaact attgattatt acaattctct
      361 taaatcgcca gatcttggaa ccaaacttta cattcacgac ttattacagg tcaataagct
      421 tttattgaat aactctcatt ctaatatata gaaggccata gaaatgtgtc gcaaactata
      481 tgataaactt tatgaaataa caggtgcaaa gcttgatttt aacgataaaa atcaagcctt
      541 tatattgctc gaagaacaaa tacctgtttg tataacagat aatgatgaat acatcttttt
      601 gaccggactt cttaatgaac atgaactttt tacagaaaat attattaatc cagaacacat
      661 actcatcctt aactactctt tgagtagaga ttatggttct tctatctgtc ttctccctga
      721 tactcatcaa tgtgttttga ccaagaaaca ctacaaaaaa tatctttcac ctgatgaact
      781 cattgaatct ttgtatgaat ttttattttg tattaaacta accattgcaa acatcacttc
      841 agaagtgaac taacatatag ggggtatcat gcaaattcta aacaaaatac ttccacaggt
      901 tgaattcgcg attcctcgcc catcatttaa ttcgttaagt tacaataagc ttgtaaagaa
      961 aatattatct gtttttaact taaaacaacg ctttcctcag aagaattttg gatgcccggt
     1021 gaatataaat aaaatcaggg acaacgtcat agacaaaata aaagatagta attcggggaa
     1081 tcaactattt tgttggatga gccaagagcg aacctcttat gtctcttcaa tgataaacag
     1141 aagtatagat gaaatggcca tacacaatgg agttgttttg acctcagata acaaaaaaaa
     1201 catatttgca gctattgaaa aaaaattccc ggatataaaa cttgatgaaa aatcagcaca
     1261 aacatctatc agtcatacag cactaaacga gattgcctca tctggcctca gagcaaagat
     1321 tttaaaacgc tatagcagta acatggattt gtttaacaca caaatgaagg atcttacaaa
     1381 tctagtatcc agttccgtct acgataaaat atttaatgaa tcaacaaaag ttttacaaat
     1441 agaaataagc gcagaggttc taaaggccgt ataccgtcaa agcaatacaa attaaatctg
     1501 tttatattaa atcttatact tattaaagta aagcagacaa aaaggagacc ttatgtcttt
     1561 aaatatcact gaaaatgaaa gcatctctac tgcagtaatt gatgcaatta actctggcgc
     1621 tacactgaaa gatattaatg caa
//
LOCUS       DAEEBB010000328         1641 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEEBB010000328 DAEEBB010000000
VERSION     DAEEBB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.334"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..891
                     /locus_tag="KLF07_004454"
                     /pseudo
     CDS             1..891
                     /locus_tag="KLF07_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    <1..79
                     /locus_tag="KLF07_004454"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1056..>1641
                     /locus_tag="KLF07_004455"
     CDS             1056..>1641
                     /locus_tag="KLF07_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD9834238.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          437 a          376 c          421 g          407 t
ORIGIN      
        1 aaaaatgatg ccactgctgg ataagctgcg tgagcagtac ggggtcggac cgctatgcag
       61 cgaactgcat attgccccgt caacgtatta ccactgtcag caacagcgac atcatccgga
      121 taaacgcagt gcccgtgcgc agcgcgatga ctggctgaag aaagagatac agcgcgtata
      181 cgatgaaaat cacaaggtat acggtgtaaa gtctggcgtc agttgttacg ggaaggtatc
      241 agagtggcca gatgcactgt ggcacgtctc atggcggtta tgggacttgc cggtgttctc
      301 cggggtaaaa aggtccgtac gaccatcagc cggaaagccg ttgccgcagg cgaccgcgta
      361 aaccgtcagt tcgtggcaga acgacctgac cagctgtggg tggctgattt tacttacgtc
      421 agcacatggc agggcttcgt ctatgtggcg ttcatcattg atgtgtttgc cggatacatc
      481 gtgtggtgac gagtctcatc gtctatggaa acgacattcg tgctggatgc actggagcag
      541 gcgttgtggg cccgtcgccc gtctggcacc atccatcaca gcgataaagg ctctcagtat
      601 gtgtcactgg cctataaggc gcgactaaaa gaagcaaaac tgctggcatc aacagggagt
      661 actggtgact cgtatgacaa cgcgatggca gagatcatca agggccttta caaagcggag
      721 gtaatacacc gtaagagctg gaaaaaccgg acagaagtgg agctggccac actcacgtgg
      781 gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatatccc accggcagaa
      841 gcagaaaaag cttattatgc ttccatcgga aatgatgatc tggcagcctg agttcacaga
      901 taaaacactc tccaggaaac ccggggcggt tcagttcaca aaaacacatt aatgcagtaa
      961 ctatattttc ctttctggtg ataacgtcgg gttatcatta gcttcttcag ctattttact
     1021 tttatatccc ttgtaaacat catggagaat ccatagtgaa taaagtttat tctcttaaat
     1081 attgccccgt caccgggggg cttattgctg tctctgaact tgcccgcagg gtaataaaaa
     1141 agacatgccg aagattaacg catattcttc tggctggcat tccagcaatc tgtctgtgtt
     1201 actctcagat atccctggcg ggtattgtcc gttccgatat tgcctatcag atttatcgtg
     1261 atttcgccga aaacaaaggg ctttttgtac ctggtgccaa tgatattccg gtatatgata
     1321 aggacggaaa acttgtggga agactgggta aagccccaat ggccgatttc agcagtgtga
     1381 gctcaaatgg cgttgctacg cttgtatcgc ctcagtatat cgtcagcgta aagcataacg
     1441 gaggatatcg gagtgtgagc tttggtaatg ggaaaaatac atattccctt gttgaccgta
     1501 ataaccaccc ttctattgac ttccatgctc cacgtctgaa taaactggtt acagaagtta
     1561 ttccctcagc ggtaacatca gaaggaacca aagccaatgc ttataaatac actgaacgtt
     1621 acaccgcttt ttatcgggtg g
//
LOCUS       DAEEBB010000329         1618 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEEBB010000329 DAEEBB010000000
VERSION     DAEEBB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.335"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..160)
                     /locus_tag="KLF07_004456"
                     /pseudo
     CDS             complement(1..160)
                     /locus_tag="KLF07_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(159..>1618)
                     /locus_tag="KLF07_004457"
     CDS             complement(159..>1618)
                     /locus_tag="KLF07_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HBD9834239.1"
                     /translation="KGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGELNITTGGIVKA
                     RDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGV
                     FEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLIT
                     GDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPG
                     TLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFAGVAQLKDRTF
                     TLERDNTAALTHAMLQSDSENTTSVNVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYI
                     SVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPIANNPLTTLNLLEHDDSHVG
                     VQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGL
                     YVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAK"
BASE COUNT          364 a          464 c          362 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAEEBB010000330         1557 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEEBB010000330 DAEEBB010000000
VERSION     DAEEBB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.336"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            271..1386
                     /locus_tag="KLF07_004458"
                     /pseudo
     CDS             271..1386
                     /locus_tag="KLF07_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          614 a          280 c          221 g          442 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc ttatcaaatc attatcagaa tataatacca taatattgat
     1501 tattagcact ttataattat tgaatgaaaa tagattttat gtaattttaa aacaaag
//
LOCUS       DAEEBB010000331         1536 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEEBB010000331 DAEEBB010000000
VERSION     DAEEBB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.337"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(488..1012)
                     /locus_tag="KLF07_004459"
                     /pseudo
     CDS             complement(488..1012)
                     /locus_tag="KLF07_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
     gene            1267..1513
                     /locus_tag="KLF07_004460"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="KLF07_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAEEBB010000332         1523 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEEBB010000332 DAEEBB010000000
VERSION     DAEEBB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.338"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..339
                     /locus_tag="KLF07_004461"
     CDS             <1..339
                     /locus_tag="KLF07_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphatase"
                     /protein_id="HBD9834240.1"
                     /translation="LAYTIGAVPCWNCKSGKDRTGMQDAEIKREIIRKHETGQFSQLN
                     SKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKKLPLSSLELSYSERIGDSKIW
                     NMVKGYSSFV"
     gene            349..711
                     /gene="ipgE"
                     /locus_tag="KLF07_004462"
     CDS             349..711
                     /gene="ipgE"
                     /locus_tag="KLF07_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBD9834241.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            711..1169
                     /gene="ipgF"
                     /locus_tag="KLF07_004463"
     CDS             711..1169
                     /gene="ipgF"
                     /locus_tag="KLF07_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBD9834242.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            1178..>1523
                     /gene="mxiG"
                     /locus_tag="KLF07_004464"
     CDS             1178..>1523
                     /gene="mxiG"
                     /locus_tag="KLF07_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBD9834243.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRG"
BASE COUNT          534 a          190 c          341 g          458 t
ORIGIN      
        1 ttggcttata ctataggtgc tgtaccttgc tggaattgta agagtgggaa ggacagaaca
       61 ggtatgcagg atgctgaaat taaaagagaa ataataagaa aacatgaaac aggtcagttt
      121 tctcaattaa atagtaaatt atcctcagaa gagaaaagat tattttctac tattctaatg
      181 aatagtggta atatggaaat ccaagagatg aatactggtg tgcccggaaa taaagtcatg
      241 aaaaaattgc cgctatcttc gttagagcta tcttattctg aaagaatagg ggactcaaaa
      301 atatggaata tggtgaaagg gtattcgtca tttgtataag aggaatatat ggaagattta
      361 gcagatgtta tttgccgagc cttgggtatc cctttaattg atatcgatga tcaagcaatc
      421 atgcttgatg atgatgtgct aatatatatt gaaaaagaag gagattcaat aaatttatta
      481 tgcccttttt gtgcattgcc tgaaaatata aatgatctta tatatgcgtt gagcctaaat
      541 tactcagaaa agatatgctt agctactgat gatgaaggcg gaaatctaat tgcgcgatta
      601 gatcttaccg ggataaatga attcgaggat gtatatgtta atacagaata ttatatttcg
      661 cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga aggggtatta atgtcccgtt
      721 tcgtatttat tttgctgtgt tttatcccat atttagggag agctgattgt tgggataagg
      781 ctggtgaaag gtacaatatt ccatcaagtt tgttaaaagc gattgcggaa aaagaatccg
      841 gatttaataa atttgcagtc aatgttaata acaatggaag taaagattat ggtataatgc
      901 aaataaatga ctttcattct aaaagactta gagaaatggg atattctgag gaaatgctaa
      961 ttagtcatcc atgtctttct gtacattatg cagcaaagtt attaaatgag tttatgatga
     1021 tgtatggaag agggtgggag gctgttggag cctataatgc aggaacgtct ccgaaaaaga
     1081 aaaaagaaag attaaagtac gcagaggata tctatagacg ctatttgcgg atagcagcag
     1141 agagcaagca gaataatcga aggatataag aggatttatg tctgaggcaa agaactcaaa
     1201 tcttgcacca ttcaggttat tagtgaaact cacaaacggt gtcggggatg agtttccgtt
     1261 atattatggg aataacttaa ttgttctcgg acggacgata gaaacacttg agtttggaaa
     1321 cgacaatttc ccagaaaata ttattcctgt aacagatagt aaatcggatg gtataattta
     1381 tttgaccata agcaaagata atatctgtca atttagtgat gaaaaaggtg agcaaattga
     1441 tatcaattca caatttaatt catttgaata tgacgggatt tcttttcacc ttaaaaatat
     1501 gagggaggat aaatcgagag gcc
//
LOCUS       DAEEBB010000333         1516 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEEBB010000333 DAEEBB010000000
VERSION     DAEEBB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.339"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>1516)
                     /locus_tag="KLF07_004465"
     CDS             complement(<1..>1516)
                     /locus_tag="KLF07_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD9834244.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAEEBB010000334         1513 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEEBB010000334 DAEEBB010000000
VERSION     DAEEBB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.340"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..270)
                     /locus_tag="KLF07_004466"
     CDS             complement(<1..270)
                     /locus_tag="KLF07_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBD9834245.1"
                     /translation="MSDVLVSLIIVCFNAEKYIEKSLLAFINQDVGLDKFELIIVDGD
                     SSDNTISIVQDVFSKHSNIKHKIINNKKRTLATGWNIGVLEANGKF"
     gene            complement(263..>1513)
                     /locus_tag="KLF07_004467"
     CDS             complement(263..>1513)
                     /locus_tag="KLF07_004467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834246.1"
                     /translation="ENMLLEYVERKISLALSKYPKVRDVIKFFYLYIASLFGIILNKN
                     KTVIQSKIYEISIDDSEESFFGYYDHSPMSSNGRYVLFHSSAFSTKRHPKKVKYISIC
                     VKDLLNNKVYKLYDTRAFNWQQGSRLMWIDDDNIIFNDYENNGYISVVYSLSLMKVIK
                     KINYPIYDVNNYKAVTLDFSWLAKYDSDYGYYNKKSFSTDISIINLNTGGIELFLSLD
                     EMLKRTNFKCNIDVEHVVNHFMFAPDGRSVMFIHRYYTPKGKRERLIHWNLINDNVRV
                     LINESIISHCCWNGNDEIIGFFGAEIDSLNYYRLSIESCNTEKLFFDARKYSDGHPTI
                     VHNRYIISDTYPDKNRIKKLFVYDLVKNDYRELGLFYESMSFFSYSRCDLHPRISVDN
                     RFLFVDSVHSGKRKLYFMRSGICE"
BASE COUNT          552 a          278 c          169 g          514 t
ORIGIN      
        1 aaacttacca ttagcttcta gcaccccaat attccaaccc gtagcaagag ttcttttttt
       61 attattgata attttatgct taatgttgct atgtttagaa aaaacatcct gaacaataga
      121 tattgtatta tcagatgaat ccccatctac aataatcaat tcaaatttat ctaatccaac
      181 atcttgatta ataaatgcca aaagagattt ttcaatatac ttctctgcat taaagcaaac
      241 tataattaaa gatactagaa catcactcac aaataccact cctcataaaa tatagttttc
      301 ttttccctga gtgaactgaa tcaacaaaca aaaatctatt atcaaccgag atccttggat
      361 gtaagtcaca tcgagaataa gaaaaaaaac tcattgactc ataaaataat ccaagctcgc
      421 gataatcatt tttgacaagg tcataaacaa acaacttttt aattctattt ttatctgggt
      481 aagtatcaga tataatatat ctattatgaa ctatagtagg atgtccatca gaatattttc
      541 ttgcatcaaa aaacaatttc tctgtattac aggattcaat tgacaatcta taataattta
      601 gcgaatctat ttctgcacca aaaaaaccta taatttcatc attcccattc caacaacaat
      661 gactaataat cgattcattt attaggactc gaacattatc atttattaaa ttccaatgta
      721 ttaacctttc acgctttcct ttaggtgtat agtatcgatg tatgaacata acggaacgtc
      781 catcgggagc aaacataaaa tgattgacca catgttcaac atcaatatta catttaaaat
      841 tagttctctt tagcatttcg tctaaggata aaaataattc tattcccccc gtattcaaat
      901 taatgattga aatatctgta gaaaatgatt ttttattata ataaccataa tcgctatcat
      961 atttagccag ccatgagaaa tctaacgtca cagccttgta attattcaca tcataaatcg
     1021 gatagtttat tttttttata accttcatca aagacaaaga atagacaaca ctaatgtatc
     1081 cattattttc atagtcatta aaaattatat tgtcatcatc aatccacatt aatcggcttc
     1141 cctgctgcca attaaatgct cgcgtatcat atagcttata aactttgtta ttaagaaggt
     1201 cttttacgca aatagatata tacttaactt tctttggatg tcgtttagtg ctaaacgcac
     1261 tagagtggaa caatacgtac cgcccattag agctcattgg actatggtca taatagccaa
     1321 aaaatgattc ttcagaatca tcaattgaaa tctcgtatat ttttgattga ataaccgtct
     1381 tatttttatt caaaataatt ccgaataatg atgcgatata taaataaaag aacttaataa
     1441 catcccttac cttaggatac ttactcaagg ctaaggaaat ttttctttca acatattcta
     1501 acaacatatt ctc
//
LOCUS       DAEEBB010000335         1494 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEEBB010000335 DAEEBB010000000
VERSION     DAEEBB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.341"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            22..1104
                     /locus_tag="KLF07_004468"
     CDS             22..1104
                     /locus_tag="KLF07_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD9834247.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          366 a          336 c          390 g          402 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga taatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttca
//
LOCUS       DAEEBB010000336         1461 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEEBB010000336 DAEEBB010000000
VERSION     DAEEBB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.342"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="KLF07_004469"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="KLF07_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBD9834248.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="KLF07_004470"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="KLF07_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBD9834249.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          446 a          276 c          337 g          402 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt g
//
LOCUS       DAEEBB010000337         1457 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEEBB010000337 DAEEBB010000000
VERSION     DAEEBB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.343"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="KLF07_004471"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="KLF07_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBD9834250.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="KLF07_004472"
     CDS             complement(1090..1437)
                     /locus_tag="KLF07_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HBD9834251.1"
                     /translation="MTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          406 a          320 c          364 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agcagca
//
LOCUS       DAEEBB010000338         1431 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEEBB010000338 DAEEBB010000000
VERSION     DAEEBB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.344"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            14..439
                     /locus_tag="KLF07_004473"
     CDS             14..439
                     /locus_tag="KLF07_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD9834252.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /locus_tag="KLF07_004474"
     CDS             464..>1431
                     /locus_tag="KLF07_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD9834253.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAEEBB010000339         1426 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEEBB010000339 DAEEBB010000000
VERSION     DAEEBB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.345"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..118)
                     /locus_tag="KLF07_004475"
     CDS             complement(<1..118)
                     /locus_tag="KLF07_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBD9834254.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="KLF07_004476"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="KLF07_004476"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBD9834255.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="KLF07_004477"
     CDS             complement(822..>1426)
                     /locus_tag="KLF07_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBD9834256.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAEEBB010000340         1394 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEEBB010000340 DAEEBB010000000
VERSION     DAEEBB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.346"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..459
                     /locus_tag="KLF07_004478"
     CDS             <1..459
                     /locus_tag="KLF07_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD9834257.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="KLF07_004479"
     CDS             477..1115
                     /locus_tag="KLF07_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD9834258.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          308 g          360 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac actg
//
LOCUS       DAEEBB010000341         1381 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEEBB010000341 DAEEBB010000000
VERSION     DAEEBB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.347"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            539..>1381
                     /locus_tag="KLF07_004480"
     CDS             join(539..881,881..>1381)
                     /locus_tag="KLF07_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HBD9834259.1"
                     /translation="MACYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGSWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
BASE COUNT          389 a          294 c          310 g          388 t
ORIGIN      
        1 gcatgatatg tttttttgta atgcttttgt atctttgttc ggatttattt ttccatcaac
       61 aagatcaata ttgttctgaa cattaatatg atttactgct tcaggtattt tcataaaaca
      121 tcccccatta tgtgcgtttt tatctgcagt tatttagaat aatattatct aacttaatat
      181 tttcttgtat gtattataac gaacatattt taatatgttg tacgattttc actctgaaat
      241 cccctgagtt taatcacact tcctttactg aaaggaatta ttgcaacagt attatttgtt
      301 tttggatata tatcttgcgc cttgttatgt agttaatgct cagaatatga tagataaaat
      361 agagacgcag aacagggcat gctcataaca tgatgataac ataaaaaatt agtaacaata
      421 aatgaaagaa tcatgggcgc tcaacaaaaa gagagctaaa tagctgcgcg gaatagtagg
      481 ctgtgtccct taatcatctg agttatagta actgtctgtt tacacaacat attgaactat
      541 ggcttgctac gaccttcccg atgaggcatg gaccatcatc aagcccctgt tacctcctga
      601 acccgccaca ccacgggccg gacgcccatg ggctgagcac cgtaaaatca tcaatggcat
      661 gttctgggtg ttatgttccg gtgcaccatg gcgcgattta cccgaacgat atgggtcatg
      721 gaaaactgtt tataaccgct ttaaccgatg gtcaaagtca ggcgtgatta atattatttt
      781 caacaggttg ctttcgctac ttgatgcaaa cggctttatt gactggtctg ccaccgcgct
      841 ggatggcagt aatatccggg cgctgaaatg tgccgccggt gctcaaaaaa acatcccgat
      901 atcgacggag ataatgggcc gggtcgctct cgcggcggtt ttggcaccaa aatccatctg
      961 gcaacagacg gaagtggcct cccgttaaat atcgtgctaa gccccggaca agctcatgaa
     1021 agccagttcg cgcaacgact tctggacggg attggcgttc agcgtcagaa cggcagcatg
     1081 aaacgccgtg gacatgcggt actggctgat aaagcgtatt cagggcgcgc attgcgcaac
     1141 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
     1201 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
     1261 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
     1321 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
     1381 t
//
LOCUS       DAEEBB010000342         1381 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEEBB010000342 DAEEBB010000000
VERSION     DAEEBB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.348"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..304)
                     /locus_tag="KLF07_004481"
     CDS             complement(<1..304)
                     /locus_tag="KLF07_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834260.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="KLF07_004482"
     CDS             complement(1125..1337)
                     /locus_tag="KLF07_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HBD9834261.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          449 a          288 c          261 g          383 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 a
//
LOCUS       DAEEBB010000343         1376 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEEBB010000343 DAEEBB010000000
VERSION     DAEEBB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.349"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            3..983
                     /locus_tag="KLF07_004483"
     CDS             3..983
                     /locus_tag="KLF07_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBD9834262.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="KLF07_004484"
     CDS             998..>1376
                     /locus_tag="KLF07_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD9834263.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAEEBB010000344         1345 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEEBB010000344 DAEEBB010000000
VERSION     DAEEBB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.350"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="KLF07_004485"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="KLF07_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBD9834264.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="KLF07_004486"
     CDS             complement(478..897)
                     /locus_tag="KLF07_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD9834265.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1270..>1345
                     /locus_tag="KLF07_004487"
     CDS             1270..>1345
                     /locus_tag="KLF07_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBD9834266.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALD"
BASE COUNT          405 a          321 c          277 g          342 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat
     1141 catattaata gaaataaaga cattgacgca tcccgcccgg ctaactatga attagatgaa
     1201 gtaaaattta ttaatagttg taaaacagga gtttcattac aatttatata tttaaagagg
     1261 cgaatgatta tgactgaaat cgttgcagat aaaacggtag aagtagttaa aaacgcaatc
     1321 gaaaccgcag atggagcatt agatc
//
LOCUS       DAEEBB010000345         1341 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEEBB010000345 DAEEBB010000000
VERSION     DAEEBB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.351"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..184
                     /locus_tag="KLF07_004488"
     CDS             <1..184
                     /locus_tag="KLF07_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834267.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="KLF07_004489"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="KLF07_004489"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBD9834268.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAEEBB010000346         1334 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEEBB010000346 DAEEBB010000000
VERSION     DAEEBB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.352"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>1334
                     /gene="ipaA"
                     /locus_tag="KLF07_004490"
     CDS             <1..>1334
                     /gene="ipaA"
                     /locus_tag="KLF07_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBD9834269.1"
                     /translation="LSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQA
                     KLAFDSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQD
                     GLPTPPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPEN
                     DAQSPTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNH
                     TPDNSDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSD
                     TTKSSASLSHRVASQINKFNSNTDSKVLQTDFFSRNGDTYLTRETIFEASKKVTNSLS
                     NLISLIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDK
                     NHAIYEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYK"
BASE COUNT          519 a          273 c          204 g          338 t
ORIGIN      
        1 ctttatcatt gggacaaaaa ctcctcaata ctgaagtatc atcatttatc aagaataata
       61 ttcttgctga attaaagtta agtaatgaaa ctgtttcatc tctcaaacta gatgatttag
      121 ttgacgcaca ggcaaaactt gcctttgata gtttgcgcaa tcaacgtaaa aatactattg
      181 atagtaaagg atttggtata ggtaaactgt caagagactt aaatacagta gccgtgtttc
      241 ctgagctgtt gagaaaagtc cttaatgata ttttagaaga tataaaagat tcgcatccta
      301 tccaagatgg cctccctaca cctcccgaag atatgccaga tggcggacca acccccggag
      361 ccaatgagaa aacatcccaa cctgtaattc actatcatat aaataatgat aatagaactt
      421 acgataatag agtttttgac aacagagtat atgacaatag ctatcacgag aacccagaaa
      481 atgatgcaca gtctcctact tctcagacaa acgatctatt atcccgtaac ggaaactcat
      541 tactaaatcc acaaagagca ctagttcaaa aagtaacttc cgttctacca cactctatat
      601 cagatactgt ccagacattt gcaaataatt cagctttaga aaaggttttc aaccatactc
      661 cagataattc ggatggaata ggttcagacc tgttaactac gagtagtcaa gaaagatctg
      721 caaataactc tctttctcgg ggacacaggc ctctgaacat acagaactct tcaaccaccc
      781 cccctctcca cccggaagga gtgacaagca gtaatgataa ctcatcagat acaactaaaa
      841 gtagcgcttc tctttctcat agagtagctt cgcaaatcaa taaattcaac tcaaacactg
      901 attcaaaagt acttcagact gattttttct caagaaatgg agacacatat ttaacacggg
      961 aaacgatatt tgaagcgtca aaaaaagtaa caaactccct aagtaatctt atatctctca
     1021 ttggaactaa atcaggaaca caagaacgag agttacagga aaaatcaaag gacattacaa
     1081 aatccacaac agaacacaga ataaacaaca aattaaaagt tacagatgca aatacaataa
     1141 actacgtaac agaaaccaac gcagatacaa ttgataaaaa tcatgcgatc tatgaaaagg
     1201 caaaagaagt atctagcgcc ctcagcaagg tattgtcaaa aattgacgat acctctgcag
     1261 aattacttac agatgatata tctgatttaa aaaataacaa tgatattaca gctgaaaaca
     1321 ataatatata taaa
//
LOCUS       DAEEBB010000347         1322 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEEBB010000347 DAEEBB010000000
VERSION     DAEEBB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.353"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1153
                     /locus_tag="KLF07_004491"
     rRNA            <1..1153
                     /locus_tag="KLF07_004491"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1248..>1322
                     /gene="rrf"
                     /locus_tag="KLF07_004492"
     rRNA            1248..>1322
                     /gene="rrf"
                     /locus_tag="KLF07_004492"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          325 a          303 c          409 g          285 t
ORIGIN      
        1 cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag
       61 tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggtcagcgca
      121 agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt
      181 agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga tggccaggct
      241 gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca
      301 agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga gccttgatgt
      361 gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc ggccttgaaa
      421 taccaccctt taatgtttga tgttctaacg tggacccgtg atccgggttg cggacagtgt
      481 ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga gcacgaaggt
      541 tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag cttgactgcg
      601 agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt tctgaatgga
      661 agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata ccgcccaaga
      721 gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc tggggctgaa
      781 gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg ggtttagaac
      841 gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg ggggctgctc
      901 ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat gccaatggca
      961 ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca cgaaacttgc
     1021 cccgagatga gttctccctg accctttaag ggtcctgaag gaacgttgaa gacgacgacg
     1081 ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact aatgaaccgt
     1141 gaggcttaac cttacaacgc cgaagatgtt ttggcggatg agagaagatt ttcagcctga
     1201 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggccgta
     1261 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     1321 gt
//
LOCUS       DAEEBB010000348         1321 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEEBB010000348 DAEEBB010000000
VERSION     DAEEBB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.354"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            100..288
                     /locus_tag="KLF07_004493"
     CDS             100..288
                     /locus_tag="KLF07_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834270.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            298..>1321
                     /locus_tag="KLF07_004494"
     CDS             298..>1321
                     /locus_tag="KLF07_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBD9834271.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPE"
BASE COUNT          295 a          352 c          398 g          276 t
ORIGIN      
        1 ctgcaaattg aaaaccgccc ctgttcgtcc tgttgttgcc gcacgctccg cagaatatgt
       61 atgctctgag gacctgtttt attaaggact catcctctta tgttgacccg caaaagtata
      121 gataccgttc tgctctctgt tggtgctgag aagctctcac aacgtgaatg ggactggatg
      181 aagatgctca aacccatgga cccaccacct gcgatggtcg cggcctccat cctggagcgc
      241 cgtggcgaca cagcagccct tacccggcta caggataccg ggggttaatc cgtcctcatg
      301 ttgtccgctt ttactccccg cccgctgaaa cgtctgttca cggccaacca gtgctggacg
      361 tccttcctgg atgcgggcgg tctgcgcgat atcgaggttg aggctgtcac aaaaatgctg
      421 gcctgcggca cgcggatact gggagtgaag gaatacaact gcgataaacc tgagtgcccc
      481 cacgtcagat acgtcactaa ctcatgcggc agccgtgcct gcccgtcctg cggaaaaaag
      541 gccacagacc tgtggatagc gacacagctg aatcgtcttc ctgactgcga ctgggtacat
      601 ctggtcttca ccctgccgga cacgctgtgg ccggtgttcg aaagcaaccg gtggctgctg
      661 aatgacgtgt gccgtctggc ggtggagaat ctgctgtatg ccgcccggaa acgggggcag
      721 gaacccggta tcttctgcgc catccacacg tatggccgtc gtctcaactg gcatccgcat
      781 gtacatgtgt ctgtaacctg tggatgtctg aataagcatg gtcagtggaa aaagctgagc
      841 ttcctgaaag acgcgatgcg ttcgcggtgg atgtggaata tgcggcaacg gcttctgaaa
      901 gcgtggtcag aggggctggc gatgccggag tcgttgtcac atatcacgac ggaatcacag
      961 tggagaagcc tggtgctgaa agccggagga aaatactggc atgtgtacat gtcgaaaaaa
     1021 acggccggcg ggaggaatac ggcgcgctac ctgggtcgtt atctgaagaa gccgccgata
     1081 gcggcctccc ggctggcaca ttacaacgga ggggcgagcc tgagcttccg ttacctggac
     1141 cacaaaacgg gagaaacggc gacggaaacg ctgacacagc gtgagctggt cgcgaggctg
     1201 aaacagcaca tcccggagaa gttttttaag atggtgaggt acttcgggtt ccttgccaac
     1261 cgggtgtgtg gagagaagct gccgcaggtg taccgtgctc tggggatgga taaaccggaa
     1321 c
//
LOCUS       DAEEBB010000349         1294 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEEBB010000349 DAEEBB010000000
VERSION     DAEEBB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.355"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..1112
                     /locus_tag="KLF07_004495"
                     /pseudo
     CDS             1..1112
                     /locus_tag="KLF07_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAEEBB010000350         1294 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEEBB010000350 DAEEBB010000000
VERSION     DAEEBB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.356"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..865
                     /gene="spaL"
                     /locus_tag="KLF07_004496"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             <1..865
                     /gene="spaL"
                     /locus_tag="KLF07_004496"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBD9834272.1"
                     /translation="DSLLTCGEGQRMGIFASAGCGKTFLMNMLIEHSGADIYVIGLIG
                     ERGREVTETVDYLKNSEKKNRCVLVYATSDYSSVDRCNAAYIATAIAEFFRTEGHKVA
                     LFIDSLTRYARALRDVALAAGESPARRGYPVSVFDSLPRLLERPGKLKAGGSITAFYT
                     VLLEDDDFADPLAEEVRSILDGHIYLSRNLAQKGQFPAIDSLKSISRVFTQVVDEKHR
                     IMAAAFRELLSEIEELRTIIDFGEYKPGENASQDKIYNKISVVESFLKQDYRLGFTYE
                     QTMELIGETIR"
     gene            958..>1294
                     /locus_tag="KLF07_004497"
     CDS             958..>1294
                     /locus_tag="KLF07_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834273.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
BASE COUNT          431 a          190 c          286 g          387 t
ORIGIN      
        1 tgattcttta ctcacgtgtg gtgaaggaca gcgaatgggg atttttgcgt cagctggttg
       61 tggcaaaact tttctcatga atatgctcat tgaacatagt ggtgctgata tatatgttat
      121 tgggttaatt ggtgagcgag gtcgagaggt tacagaaacg gttgattatt tgaaaaactc
      181 tgagaaaaaa aacaggtgtg ttttagtata tgcaacttcg gattactctt cggttgatcg
      241 ttgtaatgct gcatatatag ccactgctat agccgaattt tttaggactg aaggacataa
      301 agtagcgctt tttattgatt cattaacaag gtatgccaga gcattacgtg atgtggcctt
      361 agccgctgga gaatcacctg ccagaagagg ctatccggtt tcggtttttg atagcttacc
      421 cagacttctt gaaaggccag gaaagttaaa ggcaggtggc tctattactg cattttacac
      481 tgttcttttg gaggatgatg attttgctga tccattagct gaagaggtaa gatccatttt
      541 agatggacat atatatttga gcagaaatct agcccaaaaa ggacaatttc ctgcaattga
      601 ttccttaaaa agtataagca gggtatttac acaggttgtt gatgaaaaac atcgtattat
      661 ggccgctgca tttcgggagc tactttctga aatagaagag ctaaggacaa ttattgactt
      721 tggtgaatac aaaccggggg agaatgcctc tcaggataaa atctacaaca aaatatctgt
      781 tgttgagagt tttctgaagc aagattatcg actcggtttt acttatgagc agacaatgga
      841 gcttattggt gaaacaatta gataaggttt taaaaatcaa agataaatat caacggtctg
      901 ttaaattaat tgaagcacac atattaacac taaaaaaaaa actctttgta tcgagatgtg
      961 gaggcattag ataaaaggat tatatacttc ctgcaactgg agaatgactt agaacctgta
     1021 ggcgctcaga gtgttagtca gcttttcaat acaagacgta agatcgctat agtaaaaaag
     1081 catattatcc aatatcagtc tgaacgaatt cttctaaagg gaagaattga agaaattcaa
     1141 aaagatattg atgaggcaaa tgcttctaaa agaaaactgc ttcataaaga gagtaaaata
     1201 tgtaaacgga ttggcttgat taaaagaaat aactttgcca aacagttgat acttgatgag
     1261 ttatcacaag aggatatgaa gtatggcatt agat
//
LOCUS       DAEEBB010000351         1264 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEEBB010000351 DAEEBB010000000
VERSION     DAEEBB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.357"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(23..>1264)
                     /locus_tag="KLF07_004498"
     CDS             complement(23..>1264)
                     /locus_tag="KLF07_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9834274.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          333 a          298 c          228 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtcg ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAEEBB010000352         1264 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEEBB010000352 DAEEBB010000000
VERSION     DAEEBB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.358"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..979
                     /locus_tag="KLF07_004499"
     CDS             <1..979
                     /locus_tag="KLF07_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9834275.1"
                     /translation="PLQQSLKDLERAYKNFFRKRAAFPRFKKRGQNDVFRYPQGVKLD
                     QENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPAHPSASM
                     VGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQR
                     LHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGL
                     NRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRC
                     QVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(1019..>1264)
                     /locus_tag="KLF07_004500"
     CDS             complement(1019..>1264)
                     /locus_tag="KLF07_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBD9834276.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          341 a          342 c          331 g          250 t
ORIGIN      
        1 gccattgcaa cagtcactga aagaccttga gcgggcttac aaaaacttct tccggaagcg
       61 ggctgctttt ccccgattca aaaagcgggg acagaatgat gtattccgct acccgcaggg
      121 cgttaagctc gatcaggaaa acagccgtat ttttctgccg aaactgggct ggatgcgcta
      181 ccggaatagc cgtcaggtca cgggtgttgt gaaaaatgtc actgtcagcc agtcctgcgg
      241 tacgtggtac atcagtattc agacagaaag tgaagtatcc actccggctc acccttcagc
      301 atcaatggtc gggctggatg ctggcgtggc taaactcgcc acgctgtcag atggcacagt
      361 ctttgagcct gtaaacagtt ttcagaaaaa ccagaagacg ctggcgagac ttcagcgaca
      421 gttaagccgc aaggtcaaat tcagcaacaa ctggcagaag cagaaacgca aaatacagcg
      481 actgcattcc tgtatcgcaa atatccgcag ggactacctt cacaaagtca caacggccgt
      541 cagcaaaaac cacgcaatga tagtcattga ggatttgaag gtcagcaaca tgtcaaagtc
      601 agcagcgggt acggtcagcc agccggggcg caatgtccgg gcaaaatcag gtttaaaccg
      661 ttcgatactg gatcagggct ggtatgaaat gcaccgccag cttgagtaca agcagctctg
      721 gcgtggcggt caggtgcttg ctgttccgcc agcgtataca agccagcgtt gcgcgtactg
      781 tggtcataca gcgaaagaga accgcctgtc acaaagtaaa ttcagatgcc aggtatgtgg
      841 atatacagcg aacgccgatg taaatggtgc tcgtaacatt ttagcggcgg ggcacgccgt
      901 tcttgcctgt ggagagatgg tgcagtcagg ccgcccgttg aagcaggaac ccaccgaaat
      961 gattcaggcg acagcctgaa cgtagcaggg atccacgtcc ttcagggcgt ggaggatgtc
     1021 aatatcggtt tctgtcagcg attaaattca acaccacgta acaaacgcct ccggcaatta
     1081 atccccagaa cgcagaacca atcccgacca gcgtcaatcc acttgccgtt accagaaatg
     1141 ccaccaccgc cgcgtcgcgc tcacgctcac gctcattatg cagcgcctga tacaaactgc
     1201 cgccaatggt actcaacagc gccagacctg ccagcatctg gatccaactt acgggcaggg
     1261 cagc
//
LOCUS       DAEEBB010000353         1252 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEEBB010000353 DAEEBB010000000
VERSION     DAEEBB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.359"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            52..553
                     /gene="tnpA"
                     /locus_tag="KLF07_004501"
                     /pseudo
     CDS             52..553
                     /gene="tnpA"
                     /locus_tag="KLF07_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(571..1239)
                     /locus_tag="KLF07_004502"
                     /pseudo
     CDS             complement(571..1239)
                     /locus_tag="KLF07_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          330 a          286 c          280 g          356 t
ORIGIN      
        1 ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca aatgatactt
       61 tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt cctgatgcat
      121 gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga cagaaaaact
      181 acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg aaatggatgg
      241 cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga tatccagtct
      301 ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac cagatattgc
      361 agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca gtagctgcgg
      421 aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt
      481 ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc accggataaa
      541 aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa acatggtggc
      601 atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg gcgtactttc
      661 gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt tattagtcgc
      721 tgcatcaatc agctcacctt caaaatagag tcgagtatcc atggtacggt ggcctgtagc
      781 catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt gcagcccttc
      841 tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg gcccagcggt
      901 ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg tatagttcaa
      961 aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg gataataagt
     1021 gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt ctacccaacg
     1081 taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat tgtttaaaaa
     1141 accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag ccaacaagcc
     1201 gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc tg
//
LOCUS       DAEEBB010000354         1238 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEEBB010000354 DAEEBB010000000
VERSION     DAEEBB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.360"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..351)
                     /locus_tag="KLF07_004503"
     CDS             complement(<1..351)
                     /locus_tag="KLF07_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834277.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVN"
     gene            complement(440..>1238)
                     /locus_tag="KLF07_004504"
     CDS             complement(440..>1238)
                     /locus_tag="KLF07_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD9834278.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          329 a          269 c          241 g          399 t
ORIGIN      
        1 attaactgaa tttatatcaa tgattttttt attcttatct tttattttat ttactgtttc
       61 aatgcaatca gcggcaagtt ggagtggatt tagatttttc agatatggtc ttatgatctt
      121 tcccttactt aataaatagc atgtatagga ttcggaaagt ccagcatcta ttttgtacct
      181 cattatatta gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag
      241 tccgagttca gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgtcc
      301 ctctttagta ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc
      361 tctctgaaat tgtcttgcag taactgcact cgcagaaata gagaaaaaaa tacaattgtt
      421 aataactttg ccatattttt tagtccgtaa ctgttaatgt gctatctggc tctgaaaata
      481 ttaactcatt taatttttgc atgtcattag atgaaccagt tatacaaccc tcggaacaga
      541 atcgaggtcc taaagacgga ccgaaagcgt ttaaacaact ttgtgttaaa aatccatcac
      601 gattatatgt ttctgtattt gctgacggta ctaaacgtat accattaggt tttctattgg
      661 ttgctccagg accattaaca ttccagctcc ataccccttg aggtattgga cctctatttt
      721 gtaagtgtgt acaaccagga ttatttttac attgcatatt attaccatta ttaccagagg
      781 cgacaggaat agtaagcaca tcataagagt tatcatcatt ttgcgtatct gaaacgcatg
      841 aaagcatact taaagttatg ctgtataaac aggtcgctaa tcccaacggg tcaatatcag
      901 ttatcggatt caacggatac tgataaaaat tccatccccc cttcagtcca atcggatcct
      961 gagtgatata tcgcccctgc agcgggtcat aatagcggtg gcggttgtaa tacaggccgg
     1021 actcctcatc atactgctgc cccggcaggc ggataagctg ctgcagctga tgcgggttct
     1081 cttcattcag caggttgccc cactcatcgt attctgcgca ccattctgtt gccccttccg
     1141 tgctgataag caccagcggc aggccgcgat ggtcgcagtg gtacaggtgg atttttcgcg
     1201 ccggcgtgta caccgggtcc atctggtttt gcatctgc
//
LOCUS       DAEEBB010000355         1237 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEEBB010000355 DAEEBB010000000
VERSION     DAEEBB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.361"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="KLF07_004505"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="KLF07_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBD9834279.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1098..>1237)
                     /locus_tag="KLF07_004506"
     CDS             complement(1098..>1237)
                     /locus_tag="KLF07_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD9834280.1"
                     /translation="RNENIVLLGPSGVGKTHLAIAMGYEAFKIFYDISKISLELYHNI
                     H"
BASE COUNT          433 a          194 c          206 g          404 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ccagtaatac gatattttca ttacgtt
//
LOCUS       DAEEBB010000356         1235 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEEBB010000356 DAEEBB010000000
VERSION     DAEEBB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.362"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>1235
                     /gene="istA"
                     /locus_tag="KLF07_004507"
     CDS             <1..>1235
                     /gene="istA"
                     /locus_tag="KLF07_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HBD9834281.1"
                     /translation="FDKKLTNRCIGLTLHISASTVFEVLARFKASSLSWPLPADISHD
                     TLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSHFC
                     RCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGASNY
                     TYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVINDSYQAL
                     AEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARLRELLTD
                     MNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYS
                     VPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWT
                     PERLIEQGNRTGPSTGRVV"
BASE COUNT          306 a          350 c          323 g          256 t
ORIGIN      
        1 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
       61 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      121 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      181 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      241 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      301 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      361 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      421 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      481 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      541 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      601 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      661 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      721 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      781 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      841 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
      901 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
      961 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1021 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1081 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1141 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1201 aaaccgtaca ggtccgtcca caggccgggt ggttg
//
LOCUS       DAEEBB010000357         1233 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEEBB010000357 DAEEBB010000000
VERSION     DAEEBB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.363"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..761)
                     /locus_tag="KLF07_004508"
                     /pseudo
     CDS             complement(1..761)
                     /locus_tag="KLF07_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          308 a          299 c          308 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aac
//
LOCUS       DAEEBB010000358         1216 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEEBB010000358 DAEEBB010000000
VERSION     DAEEBB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.364"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1141
                     /locus_tag="KLF07_004509"
     CDS             <1..1141
                     /locus_tag="KLF07_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBD9834282.1"
                     /translation="NWLEKQPEGAVRPVVIESVTKIMACGTTLMGYTQWCCSSPDCCH
                     TKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPCQYWSLVFHNRWL
                     LTEMSRIAADVILEICHQADVESGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWK
                     NLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNV
                     NVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSG
                     DEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQ
                     RLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHELLARMRWCG"
BASE COUNT          287 a          279 c          387 g          263 t
ORIGIN      
        1 taactggctg gagaagcagc cggaaggggc agtacgtccg gtggtgattg agtcggtgac
       61 caaaatcatg gcgtgcggga ccacactgat ggggtataca cagtggtgct gttcatcacc
      121 ggactgttgc catacaaaaa aggtctgctt ccggtgtaaa agccgctcct gcccgcactg
      181 cggggtgaag gctggcgcac agtggataca gtatctgctg agcctggtcc ccgactgccc
      241 gtggcagcat attgtgttca cacttccctg ccagtactgg tccctggtgt tccacaaccg
      301 gtggttactg acagagatga gccgcattgc agcggatgtg atactggaaa tctgccatca
      361 ggcagatgtg gagtcgggga tattcacggt gatccacaca tgggggcgtg accagcagtg
      421 gcatccgcat atccatttat cgacaactgc cggtggtgtg acgtcgggcc acacctggaa
      481 aaatcttcat ttttacgccc gtaaggtgat gagcatgtgg cgctaccgga taacacggtt
      541 gctgtcacgg aaataccctg acctggtgat gccggatgcg ctggcagcag aaggaagcag
      601 taaacgggaa tggaatcgct tcctggacac gcattaccgc cgtggctgga atgtcaacgt
      661 atcccgggtg atggataatg ccacccatgt ggcggtgtac tttggctctt acctgaaaaa
      721 gccaccagtg ccgatgagtc gtctggagca ttatgccggt caggatgaaa tcggtctgcg
      781 ttacaacagt caccgaacaa aacgggaaga atacctgttg atgagtggag atgagttcat
      841 ggaaaggttc tcctggcatg tggcggataa ggggttccgt atggtgaggt actacggttt
      901 tttgagtccg gcgaaacggc ggttactgga agaagtggtg tacatcataa cggagacagt
      961 gagaaaaacg gcgatgcaaa tcacctggag agggatgtat cagaggttac tgaaggttga
     1021 cccgctgaag tgcgtgctgt gcgggagtca gatgcgtttt acggggctga agcggggcta
     1081 ccgtctggca gagcaggtcc tgatgcatga gctgctggcc cggatgcggt ggtgcggctg
     1141 agagccgcag aggggaagtt gcgtccattt tgagggaaac ggagcaaaaa accgccattc
     1201 ataccctgaa tcaatc
//
LOCUS       DAEEBB010000359         1213 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEEBB010000359 DAEEBB010000000
VERSION     DAEEBB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.365"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            231..>1213
                     /gene="gspA"
                     /locus_tag="KLF07_004510"
     CDS             231..>1213
                     /gene="gspA"
                     /locus_tag="KLF07_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBD9834283.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          329 a          249 c          310 g          325 t
ORIGIN      
        1 agcatcttta ttatgattcg ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt
       61 tctaataaaa tagattgggt gggttgtata ttgacgttag tataaatagt attaatgccg
      121 cttgcatgaa tcaagcaaaa tttatttata ttagtaatat gtatttaatt aataaataca
      181 tacattattt ttatgattaa ggataatcaa taattaaaac aggaagttct atgtctacga
      241 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
      301 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      361 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      421 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcctggacg cgagatgaga
      481 tggcggggca attactgcat gcgcttcatc gaactgatag tcgctgccct cttattatta
      541 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      601 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt
      661 ttaaaaaaca ggacattgaa cttgtctcga caggacgtct gacggagcac gaacttaagg
      721 caagcatcct tgaaggtcag aatattgacc aacccgatct ccttttaacc tccagagttc
      781 tgaaacggat agctttatta tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa
      841 caattcgctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      901 tgatttaccg catattgggt gataaccgcc cccggaaaat gcagcttgcc gtggtaatga
      961 gtggaaccat tatcgctatt acctgtggtt ggctactgct gtcctctttc acagctccgt
     1021 tccccgttcc tgcgtggctg gtacccgtaa cgccagtagt taaacaagat atgactaagg
     1081 atattgctca tgtggtgatg cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg
     1141 ggtacgaggt cccagctgat tcggcctggt gcgatcaggc agtaagagcg gggctggcat
     1201 gtaaaagtgg gaa
//
LOCUS       DAEEBB010000360         1211 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEEBB010000360 DAEEBB010000000
VERSION     DAEEBB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.366"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..680)
                     /locus_tag="KLF07_004511"
     CDS             complement(<1..680)
                     /locus_tag="KLF07_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD9834284.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="KLF07_004512"
     CDS             complement(996..>1211)
                     /locus_tag="KLF07_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBD9834285.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAEEBB010000361         1211 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEEBB010000361 DAEEBB010000000
VERSION     DAEEBB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.367"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            54..1211
                     /locus_tag="KLF07_004513"
                     /pseudo
     CDS             54..1211
                     /locus_tag="KLF07_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          273 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca g
//
LOCUS       DAEEBB010000362         1200 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEEBB010000362 DAEEBB010000000
VERSION     DAEEBB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.368"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            223..1038
                     /locus_tag="KLF07_004514"
     CDS             223..1038
                     /locus_tag="KLF07_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834286.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          365 a          230 c          234 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagcag
//
LOCUS       DAEEBB010000363         1190 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEEBB010000363 DAEEBB010000000
VERSION     DAEEBB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.369"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>1190
                     /locus_tag="KLF07_004515"
     CDS             <1..>1190
                     /locus_tag="KLF07_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD9834287.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFA"
BASE COUNT          265 a          339 c          375 g          211 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAEEBB010000364         1189 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEEBB010000364 DAEEBB010000000
VERSION     DAEEBB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.370"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..294)
                     /locus_tag="KLF07_004516"
     CDS             complement(<1..294)
                     /locus_tag="KLF07_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD9834288.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(342..914)
                     /locus_tag="KLF07_004517"
     CDS             complement(342..914)
                     /locus_tag="KLF07_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBD9834289.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          379 a          246 c          220 g          344 t
ORIGIN      
        1 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
       61 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
      121 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
      181 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
      241 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      301 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      361 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
      421 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
      481 ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
      541 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
      601 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
      661 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
      721 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
      781 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
      841 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
      901 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
      961 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
     1021 aaaataatat taattacgca aaaataaaat ataaattcat tgtttaaata ttatttaaac
     1081 agtaaaaaat caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa
     1141 agatagataa atttattaat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DAEEBB010000365         1189 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEEBB010000365 DAEEBB010000000
VERSION     DAEEBB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.371"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            119..376
                     /locus_tag="KLF07_004518"
     CDS             119..376
                     /locus_tag="KLF07_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834290.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="KLF07_004519"
     CDS             376..750
                     /locus_tag="KLF07_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD9834291.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1189
                     /locus_tag="KLF07_004520"
     CDS             747..>1189
                     /locus_tag="KLF07_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD9834292.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAK"
BASE COUNT          334 a          260 c          332 g          263 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtg
//
LOCUS       DAEEBB010000366         1179 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEEBB010000366 DAEEBB010000000
VERSION     DAEEBB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.372"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="KLF07_004521"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="KLF07_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBD9834293.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="KLF07_004522"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="KLF07_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          356 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctt
//
LOCUS       DAEEBB010000367         1175 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEEBB010000367 DAEEBB010000000
VERSION     DAEEBB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.373"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..242)
                     /locus_tag="KLF07_004523"
     CDS             complement(<1..242)
                     /locus_tag="KLF07_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834294.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKR"
     gene            complement(281..490)
                     /gene="hha"
                     /locus_tag="KLF07_004524"
     CDS             complement(281..490)
                     /gene="hha"
                     /locus_tag="KLF07_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD9834295.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(536..997)
                     /locus_tag="KLF07_004525"
     CDS             complement(536..997)
                     /locus_tag="KLF07_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBD9834296.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
BASE COUNT          318 a          287 c          216 g          354 t
ORIGIN      
        1 cgcttcatat ccacaattct gaacagtcct ttcataaaac tgacttccct ttctgtcagc
       61 agcgtcttct gacgttgcca gtattcaccg ctggacagta ttcgctctgc atcaccggtc
      121 cgtatacctg ccgcatgcaa ttgcatcagc aaaggagaat gctgatttac acttctgagg
      181 ataatccgga taagtagcgc tgcaataatc agaacaatga gaataaaaat aatcaacttc
      241 attatttatc tgctcctggc aattaattat tgctgaatta ttaccttaca tatttccata
      301 tctcttttgg tatcctgtca tataactttc cggtgattaa ttccgctaac cggtggtctg
      361 ctgctgaatt aaatgattca cgttcactgt ttgataaact gtctctgttt ttatgaataa
      421 ttttttccag cgtattgact gatgaacatc gccgtaactg atacagccat tcctgttttg
      481 tttttgccat ttttattccc gctgagaaca gatgaatgat aggattgcca tcgctttatt
      541 tacggtgcat ccatatccag gggggaaccg gatcagcatc tgaccagagc ccacgttttt
      601 gttgtctggc ttcgttctga aggacaggta gcacgggatc tgtgttgtac tgctcatata
      661 cccaggcagc cccggcccgg accataaact gattaatatt cattccgtct ggtgcataaa
      721 cctgcccgag aatacgacca taacgatctc gctgaaaata tgtgacggta accgtttttc
      781 ctgcgaccag tgatttcatc atgtctgttg accaccggcc ataatcctgt ttcttttccg
      841 gtgcatcaat atttaccagc ctgatccgca ccgccttcag tgagtccatg acctcgatgg
      901 tatcaccgtc cagtacacga acaacccgtc catgaatatc tgcagacaat accggccatg
      961 aaaagataaa taataccagt ggaatatatt tcctcatgtt attctccata tcagataaat
     1021 ggcacctgta tttcagcccc cctgaatagg gcagcacaca ttgaataaca ccttattttg
     1081 tgaaattaat cacaaaatat aaaaaatcga cttaatgcgg aaaagactct gtgctactca
     1141 gaactgtccg gtcatactgc ccgaccggat attaa
//
LOCUS       DAEEBB010000368         1162 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEEBB010000368 DAEEBB010000000
VERSION     DAEEBB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.375"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            248..493
                     /locus_tag="KLF07_004526"
     CDS             248..493
                     /locus_tag="KLF07_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834297.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..1142)
                     /locus_tag="KLF07_004527"
     CDS             complement(510..1142)
                     /locus_tag="KLF07_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBD9834298.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          276 g          257 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cgggggtcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAEEBB010000369         1155 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEEBB010000369 DAEEBB010000000
VERSION     DAEEBB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.376"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..399
                     /locus_tag="KLF07_004528"
     CDS             <1..399
                     /locus_tag="KLF07_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBD9834299.1"
                     /translation="NNLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESR
                     LINHSEMVISYYGGKISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGN
                     YTPVERNLSRLNEGMQYAKRHFTGIQTSCL"
     gene            341..1036
                     /gene="mxiN"
                     /locus_tag="KLF07_004529"
     CDS             341..1036
                     /gene="mxiN"
                     /locus_tag="KLF07_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBD9834300.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            1029..>1155
                     /gene="mxiM"
                     /locus_tag="KLF07_004530"
     CDS             1029..>1155
                     /gene="mxiM"
                     /locus_tag="KLF07_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBD9834301.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIM"
BASE COUNT          425 a          136 c          238 g          356 t
ORIGIN      
        1 aataatctca taattaataa atatgatcta aattgcgata ttgaaccttt aagcggggta
       61 accgctatgt ttgttgccaa ctggaattta cttccagctg ttgcttattt tatcggtagt
      121 caagagtctc ggttaattaa tcatagtgag atggttatta gttattatgg gggcaaaatc
      181 agtaagcaag gggaggccgc tattcgaagt ggtttttggc atcttattgc gtggaaggaa
      241 aatatatctg ttggtatata tgaaagaata aacttattat ttaatccaat tgctttggag
      301 ggaaattata cgcctgtaga aagaaatttg tcccgattaa atgaaggtat gcaatatgca
      361 aaaaggcact ttaccggtat ccagacatca tgcctatgat ggtgttgtta taaagagaat
      421 tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact gagagtaaga agaaagcaat
      481 ttgtgttata aaggatgcta cgaaaaaagc tgaatcactg cgtatagatg ctgtatgtga
      541 tggttatcaa attggtatac agactgcttt tgagcatatt attgactata tttgtgaatg
      601 gaagttaaag caaaatgaaa acagaagaaa tattgaggac tatataacta gtttgctttc
      661 agaaaattta catgatgaga ggatcattag cactttattg gagcagtggc ttagttcgtt
      721 gcgtaataca gtaacagaat taaaagtggt tttgcccaag tgtaatttag cactgaggaa
      781 aaagttagaa cttgatctcc ataaatatcg ctcagatgtc aagattattt taaaatattc
      841 tgaagggaat aattatatat tctgtagtgg taatcaagta gtggagttta gtcctcagga
      901 tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta acaaaaaatg ataaaaaata
      961 tttcaaagag ttagcacata agaaactgag acaaattgca gaagacttgt taaaagagaa
     1021 tcctgttaat gattaatcaa ataaatgcaa gcaatgctct gcagcaaaga ttaaatagtg
     1081 aagaatttgt taatttaaat gagagattat catcttcgca atcatttgat gaggatataa
     1141 tctatgaaat aatgc
//
LOCUS       DAEEBB010000370         1148 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEEBB010000370 DAEEBB010000000
VERSION     DAEEBB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.377"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..190
                     /locus_tag="KLF07_004531"
     CDS             <1..190
                     /locus_tag="KLF07_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD9834302.1"
                     /translation="VREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTN
                     NYRIALRKYQHYQRQTCT"
     gene            323..1123
                     /gene="tcyJ"
                     /locus_tag="KLF07_004532"
     CDS             323..1123
                     /gene="tcyJ"
                     /locus_tag="KLF07_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD9834303.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          308 g          274 t
ORIGIN      
        1 ggtgcgtgaa tatgtcgttc gtctgcgccg tttgggtaat catctacacg agcaaaatat
       61 ttccctcgat cagctgcagg acggtttcct tgatgaaatc ctcgccccgt ggctgcccac
      121 caccagcacc aacaattacc gcattgcgtt acggaagtat caacactatc agcgtcaaac
      181 gtgtacctga cttgtgcaga aatcctccag tctgcctgct tctgatatat attaaataag
      241 aataagatgt agcggagttg tttttgtgtt tacaaacaat ggctctacac tgcaaacaga
      301 cataacaaca ttcggggtga atatgaaatt agcacatctg ggacgtcagg cattgatggg
      361 tgtgatggcc gtggcgctgg ttgcgggtat gagcgttaaa agttttgcag atgaaggtct
      421 gcttaataaa gtcaaagagc gcggcacgct gctggtaggg ctggaaggaa cttatccgcc
      481 gttcagtttt cagggggatg acggcaaatt aaccggtttt gaagtggaat ttgcccaaca
      541 gctggcaaaa catcttggcg ttgaggcgtc actaaaaccg accaaatggg acggtatgct
      601 ggcgtcgctg gactctaaac gcattgatgt ggtgattaac caggtcacca tttctgacga
      661 gcgcaagaaa aaatacgatt tttctacccc ttacaccatt tccggtattc aggcgctggt
      721 gaaaaaaggt aacgaaggca ccattaaaac agccgatgat ctgaaaggca aaaaagtggg
      781 tgtcggtctg ggcaccaact atgaagagtg gctgcggcag aatgttcagg gtgtcgatgt
      841 gcgtacctat gatgatgacc cgaccaaata tcaggatctg cgcgtaggcc gtatcgatgc
      901 gatcctcgtt gaccgtctgg cggcgctgga tctggtgaag aaaaccaacg atacgctggc
      961 agtaaccggt gaagcatttt cccgtcagga gtctggcgtg gcgctgcgta aaggaaatga
     1021 agatctgctg aaagcagtga atgatgcaat tgcggaaatg caaaaagatg gcactctgca
     1081 agccctttcc gaaaaatggt ttggtgctga tgtgaccaaa taatcagcat aatgacaaaa
     1141 aagggcgc
//
LOCUS       DAEEBB010000371         1126 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEEBB010000371 DAEEBB010000000
VERSION     DAEEBB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.378"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..1006
                     /gene="ospD3"
                     /locus_tag="KLF07_004533"
     CDS             <1..1006
                     /gene="ospD3"
                     /locus_tag="KLF07_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBD9834304.1"
                     /translation="RWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELL
                     LAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLS
                     QALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHA
                     DAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEA
                     KLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAE
                     GPNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS
                     "
BASE COUNT          388 a          201 c          228 g          309 t
ORIGIN      
        1 cagatggcct aataatttac ttcaccccaa agttatttat cacgcgatgc gtatgggatt
       61 gactgagcta atccaaaaag taacaagagt cgtacaacta tctgaccttt cagacaatac
      121 gttagaatta cttttggcag ccaaaaatga cgatggtttg tcaggattgc ttttagcttt
      181 acaaaatggg cattcagata caatcttagc atacggagaa ctcctggaaa cttctggact
      241 taaccttgat aaaacggtag aactactaac tgcggaagga atgggaggac gaatatcggg
      301 tttatcccaa gcacttcaaa atgggcatgc agaaactatc aaaacatacg gaaggcttct
      361 caagaagaga gcaataaata tcgaatacaa taagctgaaa aatttgctga ccgcttatta
      421 ttatgatgaa gtacacagac agatacccgg actaatgttt gctcttcaaa atggacatgc
      481 agatgctata cgcgcatacg gtgagctcat tcttagcccc cctctcctca actcagagga
      541 tattgtaaat ttgctggcct caaggagata tgacaatgtt cccggacttc tgttagcatt
      601 gaataatgga caggctgatg caatcttagc ttatggtgat atcttgaatg aggcaaaact
      661 taacttggat aaaaaagcag agctgttaga agcgaaagat tctaatggtt tatctggatt
      721 gtttgtagcc ttgcataatg gatgtgtaga aacaattatt gcttatggga aaatacttca
      781 cactgcagac cttactccac atcaggcatc aaaattactg gcagcagaag gcccaaatgg
      841 ggtatctgga ttaattatag cttttcaaaa taggaatttt gaggcaataa aaacttatat
      901 ggaaataata aaaaatgaaa atattacacc tgaagaaata gcagaacact tggacaaaaa
      961 aaatggaagt gattttctgg aaattatgaa gaatataaaa agctgaatat tatctcgtgg
     1021 tgatacactg aatttgaggt aaccggctca tttaaaccgt ctggtctgtt acctccggtt
     1081 ccacaaaaat aatgtccatc attttgaagt ggacactatc gtatgg
//
LOCUS       DAEEBB010000372         1117 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEEBB010000372 DAEEBB010000000
VERSION     DAEEBB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.379"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            84..1115
                     /locus_tag="KLF07_004534"
     CDS             84..1115
                     /locus_tag="KLF07_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBD9834305.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAEEBB010000373         1107 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEEBB010000373 DAEEBB010000000
VERSION     DAEEBB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.380"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            255..>1107
                     /gene="virB"
                     /locus_tag="KLF07_004535"
     CDS             255..>1107
                     /gene="virB"
                     /locus_tag="KLF07_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBD9834306.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLS"
BASE COUNT          438 a          196 c          168 g          305 t
ORIGIN      
        1 ttatttctgt agtcaaaaat agtacaaaat caatatagta attgataagc attttttcat
       61 ctatggagct ctcacatcag agctccacaa gaatattatt cttttatcca ataaagataa
      121 attgcatcaa tccagctatt aaaatagtaa tttttaagac taccgttgac tatcatcaac
      181 agttacatac aatcttgctc acactgcatt taacttttgt caatataaat caatatgaat
      241 aaacagggtg tgatatggtg gatttgtgca acgacttgtt aagtataaag gaaggccaaa
      301 agaaagagtt tacactccat tctggtaata aagtttcctt tatcaaagcc aagattcctc
      361 ataaaaggat ccaagattta accttcgtca accaaaaaac gaatgtacgc gatcaagaat
      421 ccctaacaga agaatcatta gccgatatca taaaaactat aaagctacaa caattcttcc
      481 ctgtaatagg aagggagatt gatggtagaa ttgaaattct ggatggcact cgtagaagag
      541 catctgcaat atatgcagga gcagatcttg aagttctata ttcaaaagaa tatatatcta
      601 ctcttgatgc cagaaaacta gcaaacgata tacaaacagc aaaagagcat agcatccgag
      661 aacttggtat cggtcttaat tttctgaaag tatcagggat gtcctataaa gacatagcca
      721 aaaaagagaa tctgtctcgc gcgaaagtca ctcgtgcctt tcaggcagca agcgttccac
      781 aggaaataat atctctattt ccaatcgcgt cagaacttaa ctttaatgac tacaagatat
      841 tattcaatta ttataaagga cttgaaaagg ctaatgaatc tcttagttct acactaccaa
      901 tattaaagga agaaataaaa gaccttgata caaatttgcc cccggacata tataaaaaag
      961 aaattttaaa catcataaag aaaagcaaaa acagaaagca aaacccttcg ctaaaagttg
     1021 actcgttatt tatttctaaa gacaaacgga cttacataaa aagaaaagag aataaaacaa
     1081 acagaactct aatatttaca ttatcta
//
LOCUS       DAEEBB010000374         1099 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEEBB010000374 DAEEBB010000000
VERSION     DAEEBB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.381"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..191)
                     /locus_tag="KLF07_004536"
     CDS             complement(<1..191)
                     /locus_tag="KLF07_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD9834307.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDP"
     gene            435..560
                     /gene="blr"
                     /locus_tag="KLF07_004537"
     CDS             435..560
                     /gene="blr"
                     /locus_tag="KLF07_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBD9834308.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            825..1040
                     /gene="ydgT"
                     /locus_tag="KLF07_004538"
     CDS             825..1040
                     /gene="ydgT"
                     /locus_tag="KLF07_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBD9834309.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          296 a          245 c          226 g          332 t
ORIGIN      
        1 ttgggatcgt taaacagatg cttcggctca gagacaaccg taaccgttgg ccagtcggct
       61 tttacttttg tttcatcact gctggagatt accgccagtt ccagcccggg cgtgcccgca
      121 atcaggggcg catggaaggt tttgctcgca taaccatacc caatcaaccc aacacggatg
      181 ttgtcgctca tgtcattgcc tctcattgaa gtatgatggc tatttgacac tatcctttac
      241 ccacgctcaa cagtttaata acctgccagc aataagggat gttgtttaac ttaagtcaaa
      301 aaaaatagcg aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct
      361 tgctggagct attattgcta agtaacattt accccctgaa gttaatggat caatcaagag
      421 agatgtgggc tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt
      481 cagtcattct tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagtg
      541 cttcggtaca acacaagtaa gctctgcact tgtggagcga catgctgccc gtccgggtgc
      601 atgttttcac ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg
      661 attgtcaggg atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt
      721 tgccatcagc gcattcttat tattgtaata acacaagcgt tcacttttgg tgaaatttta
      781 tcatcactct acccctttgg tattaattat tttatggacc ttttatgact gttcaggact
      841 acttattaaa atttcgcaaa atcagttcac tcgaaagtct ggaaaaactc tacgaccatc
      901 ttaattacac cctgacggac gatcaggaac tgatcaatat gtatcgtgct gccgatcacc
      961 gtcgcgcaga gctggtttct ggcgggcgtt tgtttgacct cggccaggta ccgaagtccg
     1021 tctggcacta tgtccaataa agaaagtagc gatcggttat aaaagcctta taataacgcc
     1081 tctaaaatta gatttgcct
//
LOCUS       DAEEBB010000375         1085 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEEBB010000375 DAEEBB010000000
VERSION     DAEEBB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.382"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..643)
                     /locus_tag="KLF07_004539"
     CDS             complement(<1..643)
                     /locus_tag="KLF07_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD9834310.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="KLF07_004540"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="KLF07_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBD9834311.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAEEBB010000376         1082 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEEBB010000376 DAEEBB010000000
VERSION     DAEEBB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.383"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..164
                     /locus_tag="KLF07_004541"
     CDS             <1..164
                     /locus_tag="KLF07_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9834312.1"
                     /translation="VGYVSFREAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWK
                     NSNSVASFC"
     gene            complement(188..880)
                     /gene="ospZ"
                     /locus_tag="KLF07_004542"
     CDS             complement(188..880)
                     /gene="ospZ"
                     /locus_tag="KLF07_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HBD9834313.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYGK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RLSQIKARMPVT"
     gene            936..1082
                     /locus_tag="KLF07_004543"
                     /pseudo
     CDS             936..1082
                     /locus_tag="KLF07_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          328 a          220 c          187 g          347 t
ORIGIN      
        1 tggtgggtta cgtaagcttc agagaggcag ctcacgccat aacggactat atcgttggat
       61 attacagcgc actaagaccg cacgaatata acggtgggtt acccccaaac gaatcggaaa
      121 atcgatactg gaaaaactct aactcggtgg ccagtttttg ttgaccactt catacaatta
      181 atatacatta agtaacaggc attcgagcct tgatttgaga aagcctgtca ttcatttgtt
      241 ttatgtagct atctatttcc tctgatgcat aactaaaaag ttctcccatt tcacgagcaa
      301 taataatctc agatttaata gactttaact ctggcgactc atctcttaac acttgcataa
      361 tctctctttt tatatcatat agttctataa accgatttgc ccgtagttct ccttggggag
      421 aatcatttct gaatacaaca attatataca aaaaatcact attttcttta gggaagatct
      481 tacaactaat ctctgggagc tcacataatg catgtaagtc aggatggtac aaactatcca
      541 cagaaaattg ccccatagga cgttttccag atataactgt caaccagctt ccttccggtg
      601 tactggcttc gtctctggaa ataggctgaa aatcttccgg acatggagaa tacattgtct
      661 tcatataatt aagtcgtctg gatttctcat taataaactc ctctattttt aattgtgcca
      721 tacttctggt tataccagag atatacttct tgccatatat gtttggtgat tcattaaagt
      781 aaccatgttc aactctagga taaatatttc tcactatata ttcagtattt gcagtattta
      841 ttggaaatac gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat
      901 ataaacatta ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact
      961 ttatctatcg ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc
     1021 gctggcgacg gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc
     1081 tc
//
LOCUS       DAEEBB010000377         1071 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEEBB010000377 DAEEBB010000000
VERSION     DAEEBB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.384"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            673..>1071
                     /locus_tag="KLF07_004544"
     CDS             673..>1071
                     /locus_tag="KLF07_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834314.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDE"
BASE COUNT          320 a          224 c          264 g          263 t
ORIGIN      
        1 tcgttatgat tcacggctcg ttttgtctag ttcatttaaa gatgacagta acagattctt
       61 aatatttaat acagaacagt cgggatttta tgaaatcaat gatgtttatg tcagaaagta
      121 ataatcctct cccacaaagt gaattaaatg aatatataat gcgttcgaag tatatgtatt
      181 gagctgtcat actgagaaac aaaccttagg gcagcacaat taaagccaga aacaactatg
      241 caagaaagtt actaaataag tgctcggaac agtaaaacac tcagggggga ctcagtccgg
      301 attatgtaat cagataaatt gccaaacatc acaaatcacc agccggactg agtgatgccg
      361 agcattgcac caataccccg tgatgaacga tgcctgatgc agaaagctcc ctataaaaca
      421 aagaacaaaa actacgaccg gcgattcacc gtcatactga tgctgcatcg tggtgacact
      481 gtctgtcact ttcatgttga ctgaacaact ctgctgcgaa gaaggtgtac cgccggcggg
      541 atttgattgt ccggcgtcta gcttccactt ccctgatgga actgaaagtt gttatggccg
      601 gtcagcttgc cctgtccgca ggacatgagg tgctgtgacg gacagcacca gtaacggaaa
      661 gtgacaatga aaatgaatac tgcgctgata gttgcactaa tgtgtatgtg gtatgcggta
      721 cccgctgctg caaaagagac tttactggct atgccacgta attcaacaga acattgctat
      781 gcggagatta atgtgcacgg accatatggg gtgtatttcc gagtggtccc gcatcccccc
      841 gggggaaaga gctgggtcga atgtaacagt gactactatt actctgataa accaccggga
      901 gtacagatac tcggaacccg tgcagggtgt cgggtatacg gaatctgcgg gaccacctcg
      961 acactgcatg tggctggaag aggggttgtg tgtattaaaa atatatgcag tccacgtggg
     1021 atgataattc acaggatacg gaaacgtcct gtagtggctg taagcgatga g
//
LOCUS       DAEEBB010000378         1067 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEEBB010000378 DAEEBB010000000
VERSION     DAEEBB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.385"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(7..309)
                     /locus_tag="KLF07_004545"
     CDS             complement(7..309)
                     /locus_tag="KLF07_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834315.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(306..932)
                     /locus_tag="KLF07_004546"
     CDS             complement(306..932)
                     /locus_tag="KLF07_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HBD9834316.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
BASE COUNT          288 a          236 c          192 g          351 t
ORIGIN      
        1 aaggtgttat ctttgtttta cttctgctat caatcgtgat acctctgttt ttgatagtgt
       61 tttaagtgct tctaccgccg ccctgacaac atcagaacga ttcactctgc ctttacctga
      121 aaccatctca gccgttataa gggcatcaat gtcttttagg ttttcttcaa aaaaagaaat
      181 actcactggt tttgctttgc ttacaaccga tgatttatct gatgactttt tggtattatc
      241 aaggccagcc gtgccaccgt taataaatgc tgcctcgtca cgaggtgaag tgtttgctct
      301 atttctcaca gaataacctc tttcactaac gcttcgattt cttgctgggc tttatcattt
      361 tgcgtttcaa aaattgtcat tgattcggcc caagcatctc gatgtgcttt acgatcacat
      421 acccgggagt ttgccagatg catttcaggg taatccttaa gtacttcagc tgcctcattg
      481 gcttcattaa caaacatatt tgtgggcgtc atgtttaaaa cgagatatcc ccgaacatcc
      541 tcattaaaat cttttgctgc tgtaaaaacg gagcatgtat gaggcacaac atccaggtcc
      601 atctgagatg ggcgcaaagg agaaatgaat acatctgcgg ccatcaatcc actacgcatt
      661 tctgcgctat cacggccagc acagtctgca ataacaaaat cataggattt ttcgtgttcc
      721 tttaacatgg ctttgatgtt acctgtcgcg cctgtaaccg gaatatgagg gagatcttca
      781 gggcgattgt tgtaccaggt taatactgat ttctggtcat ctgcgtcgac aataatcact
      841 ctattgccta gtgacatcag atacgcagct atagataccg ccagtgtact tttacctacc
      901 ccaccctttt gactacctaa tactattatc attatagcct catattaagc tgttaagtta
      961 acaacttaat attgccatct gaacacgaac catgcaatta tttttgttat gttaacaact
     1021 tagtaagttg ttaagtattt gatttataga ttaattaaga taacaag
//
LOCUS       DAEEBB010000379         1059 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEEBB010000379 DAEEBB010000000
VERSION     DAEEBB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.386"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>1059)
                     /locus_tag="KLF07_004547"
     CDS             complement(<1..>1059)
                     /locus_tag="KLF07_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD9834317.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDE"
BASE COUNT          164 a          409 c          294 g          192 t
ORIGIN      
        1 ctcatcatac tcccggctgc tgcgaagccc gtccgggtac gtcaccgacg tcacctgccg
       61 ctggctgtta tagccatacc gtaccgtcct gccgtccggc agtatcactg aagtcagctt
      121 tccagacgcc atatgcagtc ggtactccgt ccgccgtccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cagctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgcacctg
      481 catcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaagga
      661 aggagctgag agagggcagg gactgtcagg ggaagataac gaagcggtgt gcggtttacg
      721 ggcctcttcc gcccgctgcg cctgtgtggt cagcaccagg tgaaaacggc ggcccgcgcc
      781 gtccatcacc cccgtcaccg cgcccgccac gtcaccctcc gctgcccggt gaaaggccag
      841 cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg gctccggcgg
      901 cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc acggcccctg
      961 aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg gcagcacctg
     1021 ccagagcgcg cttagcggct gcgaactgtg ctgcgccgc
//
LOCUS       DAEEBB010000380         1045 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEEBB010000380 DAEEBB010000000
VERSION     DAEEBB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.387"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            284..388
                     /locus_tag="KLF07_004548"
                     /pseudo
     CDS             284..388
                     /locus_tag="KLF07_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="KLF07_004549"
     CDS             728..985
                     /locus_tag="KLF07_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBD9834318.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DAEEBB010000381         1041 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEEBB010000381 DAEEBB010000000
VERSION     DAEEBB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.388"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(88..>1041)
                     /locus_tag="KLF07_004550"
     CDS             complement(88..>1041)
                     /locus_tag="KLF07_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834319.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGS
                     RLEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTETQ"
BASE COUNT          223 a          254 c          209 g          355 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa t
//
LOCUS       DAEEBB010000382         1024 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEEBB010000382 DAEEBB010000000
VERSION     DAEEBB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.389"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>1024
                     /locus_tag="KLF07_004551"
     CDS             <1..>1024
                     /locus_tag="KLF07_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD9834320.1"
                     /translation="VTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRA
                     SAFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDITRYRYDNPHSDLPCAT
                     EDATGSRKTMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRG
                     QLIAVKDTQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEY
                     DAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVT
                     HWHYDEADRLTHRTVKGETAERWRYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGE
                     RQTVHHP"
BASE COUNT          249 a          302 c          311 g          162 t
ORIGIN      
        1 gcgtcacgca gagtcagttt gacgccgtgg gcaggctcag gacacagacg gatgccgcag
       61 gccggacaac agaatacagc ccggatgtgg tgacgggcct catcacgcgc atcaccacgc
      121 cggatggcag ggcatcggcg ttttactata accaccacag ccagttaacg tcagccaccg
      181 ggcctgacgg gctggaaatg cgccgcaaat atgatgaatc cggccgtctg attcaggaaa
      241 ccgcccctga cggcgatatc acccgctacc gttatgataa tccacacagt gacttaccct
      301 gcgcaacgga agatgccacc ggcagccgga aaaccatgac atggagccgt tacggtcagt
      361 tgctgaccgt cactgactgt tccggttatg taacccgtta tgaccatgac cgttttggtc
      421 aggtgacggc ggtgcaccgc gaggaagggc tgagccagta ccgcgcatac gacagccgtg
      481 gacagttaat tgcggtgaaa gacacgcagg gccatgaaat gcggtatgaa tacaacgccg
      541 ccggtgacct gaccgccgtc attgccccgg acggcagcag aaacgggaca cagtacgatg
      601 cgtggggaaa agccatctgt accacgcagg gcgggctgac gcgcagtatg gaatacgatg
      661 ctgccggacg ggtcatccgt ctgaccagtg aaaacggcag ccacaccacc ttccgttacg
      721 atgtactcga ccggctgata caggaaaccg gctttgacgg ccgcacacag cgttatcacc
      781 acgacctgac cggcaaactt atccgcagcg aggatgaggg gctggtcacc cactggcact
      841 atgacgaagc agaccgcctc acgcaccgca ccgtgaaggg tgaaaccgca gagcggtggc
      901 ggtacgacga acgcggctgg ctgacagaca tcagccatct cagcgaaggg caccgggtga
      961 cggtgcatta cgggtatgat gagaaaggcc ggctgaccgg tgagcgtcag acggtgcatc
     1021 accc
//
LOCUS       DAEEBB010000383         1023 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEEBB010000383 DAEEBB010000000
VERSION     DAEEBB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.390"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(77..865)
                     /locus_tag="KLF07_004552"
     CDS             complement(77..865)
                     /locus_tag="KLF07_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD9834321.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="KLF07_004553"
     CDS             complement(905..>1023)
                     /locus_tag="KLF07_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBD9834322.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAEEBB010000384          999 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEEBB010000384 DAEEBB010000000
VERSION     DAEEBB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.391"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>999
                     /locus_tag="KLF07_004554"
     CDS             <1..>999
                     /locus_tag="KLF07_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HBD9834323.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          233 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattgg
//
LOCUS       DAEEBB010000385          988 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEEBB010000385 DAEEBB010000000
VERSION     DAEEBB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.392"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..520)
                     /gene="ipaH4.5"
                     /locus_tag="KLF07_004555"
     CDS             complement(<1..520)
                     /gene="ipaH4.5"
                     /locus_tag="KLF07_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBD9834324.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGN"
BASE COUNT          280 a          184 c          210 g          314 t
ORIGIN      
        1 gattaccttc aatacgtaat attgttagcg ttgatggaag ctctggtaac gtctccagac
       61 tattaaaacg tgccgagagt gattgtaaat atggtggtaa tgatggcaga catgacaaat
      121 ttcgattgaa tgaaatatta agcttaatca gttgagatgg aagtgtcggg agtatattca
      181 agttgttacc atttacatga agttctgtca aaagcggaga attttcaggt agggagatta
      241 actcattgtt ggaaatatta agttcacgta tatgcaaagg gagtggtggt aaagaacgta
      301 gtttcagtaa gcttaaattg agaacaggct cccgggtatc cagacatatt ttcaataatc
      361 gaaccgcctg gatgcgattt tctgttgttg ttccttccct agcccagtca tcccagatgc
      421 ggtggtaatc tctggtacac tgttcttcta ccgataaacg agatatacat gataagccac
      481 aaagggaacg aaaaaaagaa tgattgttga tcggtttcat ttattaaccc ctcactattc
      541 atatcttggt ctattaatgc aaataaaacg agaaagtctt taaaaaaaca atcctagagc
      601 tttatcggcg ttttcattcc agctggagga atatgttcct gtctttccag taaccgtcga
      661 ttgttatatc agtctaccca tgttaatgtg accagttcta ctttcatcca gtttggattg
      721 atctttatcc cctatatttt caaacaattt ttatcattta actcattact gtctcgatat
      781 tcccatagca aacgatctgg ttggttagcc cctcaaaaaa gtcagttgca cgccgtaaat
      841 ccctgccggt attgagcgag gatttacggg ttgttgttaa ggtccgtaat cgggtcacag
      901 tttttccgga gtcaatccgg tctgcggttt atgcttatgc gacgtgatta tgaatggtgc
      961 agtcgtgagc cgttttcaga caatcgca
//
LOCUS       DAEEBB010000386          963 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEEBB010000386 DAEEBB010000000
VERSION     DAEEBB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.393"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            225..963
                     /locus_tag="KLF07_004556"
                     /pseudo
     CDS             225..963
                     /locus_tag="KLF07_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          281 a          232 c          246 g          204 t
ORIGIN      
        1 ctgtacaaaa aaccgccaac caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
       61 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgcatta tcagtcccct
      121 gccagaggtt cactacgtaa cctgaccgac aatgaagtgg cacactgaat ttggccacct
      181 gaacagaggt gatatgctca cctcagaaca acacaggtgc tccaatgaaa aaaagaaatt
      241 ttagcgcaga gtttaaacgc gaatccgctc aactggttgt tgaccagaaa tacacggtgg
      301 cagatgccgc caaagctatg gatgttggcc tttccacaat gacaagatgg gtcaaacaac
      361 tgcgtgatga gcgtcagggc aaaacaccaa aagcctctcc gataacacca gaacaaatcg
      421 aaatacgtaa gctgaggaaa aagctacaac gcattgaaat ggagaatgaa atattaaaaa
      481 aggctaccgc gctcttgatg tcagactccc tgaacagttc tcgataatcg ggaaactcag
      541 agcgcattat cctgtggtca cactctgcca tgtgttcggg gttcatcgca gcagctacag
      601 atactggaaa aaccgtcctg aaaaaccaga cggcagacgg gctgtattac gcagtcaggt
      661 acttgagcta catggcatca gccacggttc ggccggagca agaagcatcg ccacaatggc
      721 aacccggaga ggctaccaga tgggacgctg gcttgctggc aggctcatga aagagctggg
      781 gctggtcagc tgtcagcagc cgactcaccg gtataaacgt ggtggtcatg aacatgttgc
      841 tatccctaac taccttgaaa ggcagttcgc cgtgaccgag ccaaatcagg tgtggtgcgg
      901 tgatgtgacc tatatctgga cgggtaagcg ctgggcgtac ctcgccgttg ttctcgacct
      961 gtt
//
LOCUS       DAEEBB010000387          962 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEEBB010000387 DAEEBB010000000
VERSION     DAEEBB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.394"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..400
                     /locus_tag="KLF07_004557"
     CDS             <1..400
                     /locus_tag="KLF07_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBD9834325.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            574..>962
                     /locus_tag="KLF07_004558"
     CDS             574..>962
                     /locus_tag="KLF07_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834326.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          328 a          177 c          190 g          267 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgttcag agagcaccta agaccatctc tattagcggt
      781 gttgtagtgg ttgattcgac gggttcagtt ctattaacca gacaggggca acttgtagaa
      841 acctttattt ctacggttga acaatggcga gaccagaagc aaatcatcac tattgtttgt
      901 agagatggaa tagctatcac tgatagtatc attactcagt ttaaagcgag taaagagcat
      961 ac
//
LOCUS       DAEEBB010000388          958 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEEBB010000388 DAEEBB010000000
VERSION     DAEEBB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.395"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>958
                     /locus_tag="KLF07_004559"
     CDS             <1..>958
                     /locus_tag="KLF07_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HBD9834327.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          172 c          266 g          248 t
ORIGIN      
        1 attttaggat atcaagcgaa cagtaatggt aaggttatcg ttgaaaaggg tggcgagtgg
       61 ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg agctggggag
      121 gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc
      181 accggtatcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc
      241 tataatgggt atttcggtaa tggcacagtc aatatttcca ataatggtct gattaataac
      301 aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat
      361 aaagggcatt ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat
      421 gctggtgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact
      481 gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc
      541 actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag
      601 gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat
      661 gtcagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt
      721 gttgcgggcg tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt
      781 acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca
      841 tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt
      901 aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggta
//
LOCUS       DAEEBB010000389          941 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEEBB010000389 DAEEBB010000000
VERSION     DAEEBB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.396"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..941
                     /locus_tag="KLF07_004560"
                     /pseudo
     CDS             1..941
                     /locus_tag="KLF07_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          231 c          284 g          192 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag t
//
LOCUS       DAEEBB010000390          940 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEEBB010000390 DAEEBB010000000
VERSION     DAEEBB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.397"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>940
                     /locus_tag="KLF07_004561"
     CDS             <1..>940
                     /locus_tag="KLF07_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HBD9834328.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWG
                     VLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDL
                     ASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          272 a          222 c          277 g          169 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      481 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac
      541 agtcatcgct gggaaatcga actgggatac agggagataa aacagacgat gcaactgagc
      601 aggctgacgc tgagaagtaa aaagccggag cttgtggagc aagagctgtg gggtgtctta
      661 ctggcttata atctggtgag atatcagatg attaaaatgg cggaacatct gaaaggttac
      721 tggccgaatc aactgagttt ctcagaatca tgcggaatgg tgatgagaat gctgatgaca
      781 ttgcagggcg cttcaccggg acgtataccg gagctgatgc gcgatcttgc aagtatggga
      841 caacttgtga aattaccgac gagaagggaa agggccttcc cgagagtggt aaaggagagg
      901 ccctggaaat accccacagc cccgaaaaag agccagtcag
//
LOCUS       DAEEBB010000391          934 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEEBB010000391 DAEEBB010000000
VERSION     DAEEBB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.398"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(196..>934)
                     /locus_tag="KLF07_004562"
     CDS             complement(196..>934)
                     /locus_tag="KLF07_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD9834329.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          258 t
ORIGIN      
        1 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
       61 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      121 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      181 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      241 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      301 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      361 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      421 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      481 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      541 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      601 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      661 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      721 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      781 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      841 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      901 tggtgaaaca aataaaaaag tgagagtcgg acca
//
LOCUS       DAEEBB010000392          914 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEEBB010000392 DAEEBB010000000
VERSION     DAEEBB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.399"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(29..829)
                     /locus_tag="KLF07_004563"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="KLF07_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          259 a          218 c          174 g          263 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttct
//
LOCUS       DAEEBB010000393          911 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEEBB010000393 DAEEBB010000000
VERSION     DAEEBB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.400"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..382
                     /locus_tag="KLF07_004564"
                     /pseudo
     CDS             1..382
                     /locus_tag="KLF07_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            563..712
                     /locus_tag="KLF07_004565"
                     /pseudo
     CDS             563..712
                     /locus_tag="KLF07_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(739..>911)
                     /locus_tag="KLF07_004566"
     CDS             complement(739..>911)
                     /locus_tag="KLF07_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9834330.1"
                     /translation="VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
BASE COUNT          231 a          187 c          271 g          222 t
ORIGIN      
        1 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
       61 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      121 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      181 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      241 tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg tgcggaggtc agatgcggtt
      301 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      361 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      421 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      481 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      541 aatcttttgg ggatttaaat tcctatcgat ggacatgaat gcaggctata taagagcagc
      601 gcgcatccac ttacccaatg cggttgagaa aatagccttc gatcgctttc atgtggcgaa
      661 acaaccgggt gaggtggtgg ataaaacccg ccagaatgaa ccgccccggg tttcctggag
      721 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
      781 tttttctgct tctgccggag gagtatggcc cagcctttcc agcaatcgtc gattgttata
      841 ccagtccacc cacgtgagtg tggccagttc cacttctgca cggtttttcc agctcttacg
      901 gtgtattacc t
//
LOCUS       DAEEBB010000394          906 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEEBB010000394 DAEEBB010000000
VERSION     DAEEBB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.401"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KLF07_004567"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KLF07_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBD9834331.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAEEBB010000395          892 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEEBB010000395 DAEEBB010000000
VERSION     DAEEBB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.402"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..260)
                     /locus_tag="KLF07_004568"
     CDS             complement(<1..260)
                     /locus_tag="KLF07_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834332.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQRHS"
     gene            complement(442..840)
                     /locus_tag="KLF07_004569"
                     /pseudo
     CDS             complement(442..840)
                     /locus_tag="KLF07_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          214 a          188 c          191 g          299 t
ORIGIN      
        1 ttagaatgcc gttggctttg tttttcaaca attcctactt catttatctt tgtcaaccat
       61 tgcatttctg tagcagtgaa tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat
      121 tcgtatatag ctgctttttt attgaaaaca gtatatccat ctgtagtaac agtagaagat
      181 aataatacag gatttgatac ttcaaggatg atattttctt gtttttgtgg aaggcaaggg
      241 aatatagttt gtgtaagcat attgcttcct tatgttatat tttagtaaag aatttatttt
      301 ttacattata gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg
      361 atgagatgtg gagccagaaa aatggctgcg ttacgtcatt gaactgtcct tgacccacga
      421 tattcagaca gtcgttgtat cttaagttga cattacccgc tgacgtcgat attcccttgg
      481 tgagagataa cccaacgcac tatgcggatg gttttcattg taatgctcga tcgccactgc
      541 aaggttatgc aatgccgttc ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt
      601 tttcacgaac cgttctgcta ttccattact ctgagggcta ctgactgccg tcgtgcaggg
      661 ctccagattc aactctctgg cgaactgccg tgtttcatga gccgtataag ccgaaccgtt
      721 atcggccact ggattggccg ttccggtcct ttatcgccat atcgctgaca tgacatcctg
      781 caccgttgct ttgtcataat ctcccgtgcc tgctgcccag tctatggctt cacgatcgca
      841 atatggaacg taacgctgtc atcgggagaa tcgtcaatgt ggcattcggt ta
//
LOCUS       DAEEBB010000396          873 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEEBB010000396 DAEEBB010000000
VERSION     DAEEBB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.403"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>873
                     /gene="cadA"
                     /locus_tag="KLF07_004570"
     CDS             <1..>873
                     /gene="cadA"
                     /locus_tag="KLF07_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBD9834333.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGM
                     EKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKEL"
BASE COUNT          236 a          219 c          212 g          206 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
      481 tggaaaaaga cggcaccatg agcaactttg gtattccggc cagcatcgtg gcgaaatacc
      541 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
      601 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
      661 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
      721 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
      781 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg actatggtaa
      841 tgactccgta tgctgcgttc cagaaagagc tgc
//
LOCUS       DAEEBB010000397          829 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEEBB010000397 DAEEBB010000000
VERSION     DAEEBB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.404"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..238)
                     /gene="mxiE"
                     /locus_tag="KLF07_004571"
     CDS             complement(<1..238)
                     /gene="mxiE"
                     /locus_tag="KLF07_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBD9834334.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFI"
     gene            complement(368..796)
                     /gene="mxiM"
                     /locus_tag="KLF07_004572"
     CDS             complement(368..796)
                     /gene="mxiM"
                     /locus_tag="KLF07_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBD9834335.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
BASE COUNT          284 a          169 c           93 g          283 t
ORIGIN      
        1 agatgaatct taacattaac ttatcaagca tcgattcggt attacaacta tcccgaagat
       61 cattctcatg atagaaatga atacattttt cagcatggct atgctctact agagcaccac
      121 tgaaatagtc ccaaaaagca agaaattttg cacaactatt aatgttaaag cgataatgat
      181 agttcacatt atcactgaat tttatatttt gatttcggac aaaaaaaaac ccttccatgc
      241 tggagccagc tcgattatgt tacgttcttt atcttttaca agctcaacgt taactggttc
      301 atgtccacta gagtaaatgt aaaacatatt acttgtattt agacctttat atttactcac
      361 aatacaatta tatccctcga attaatttta atttaacttc cccatccttc tctataagga
      421 tggtatcatt tatcttatta ccttttaaaa caaccccgtc tttcagcata gaaaatagtt
      481 tatctatagc tatcttttgt gcatccatga agcacccatc tacttcctta acattaaagt
      541 tgcccatgaa tttacctgca ttaaaactat aaaccgcctt accactaata catttagagt
      601 aaacatttat acttttattg gttgtattaa acgaccataa taaatcattt ggaatagaaa
      661 aataatcctt acttacagga acaatatgcc attctttttc agaattagat gatgacttta
      721 aagcacaccc actcagtgtt aatagcaata tacttaaaat aaatattttc aacttattac
      781 taccatgtcg aatcatctgc ctctctccct gcttcaaaga cactaatta
//
LOCUS       DAEEBB010000398          821 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEEBB010000398 DAEEBB010000000
VERSION     DAEEBB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.405"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            6..422
                     /gene="tnpA"
                     /locus_tag="KLF07_004573"
                     /pseudo
     CDS             6..422
                     /gene="tnpA"
                     /locus_tag="KLF07_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          259 a          181 c          153 g          228 t
ORIGIN      
        1 ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca ttgtgttttc ctgatgcatg
       61 ttcacctggt ctttgtcacc aaataccgac gtcagatttt tgaccatgac gcgacagaaa
      121 aactacgcac ttacttttca aatgtatgtg ctgattttga agctgaactg gttgaaatgg
      181 atggcgaacc agatcacgtc catttgttaa ttaactatcc tcccaaactg gcgatatcca
      241 gtctggtaaa cagcctcaaa ggcgtatcgg gtaggtgact gcgacgagat cgaccagata
      301 ttgcagtcag gtattactac aaaggcgttt tgtggagtgt tggctatttt gccagtagct
      361 gcggaggtgc gccaatatcc gtcatccgcc aatacattga acaacagcaa acacctggtt
      421 aggtggaaaa ccacgcctta tatccctgcc ctgaaggacg gggttttacg gcgcaccgga
      481 taagcctgga gattatctac accagcatgg acatcattgt actatgcccc gtacatgccc
      541 catcacatcc tcaacatcaa agtattagag ccttgcaaac cattatgcgt ctcagtttgt
      601 ctcatctatc ttacgcaaaa aaaaatagaa ctaatatcaa gcggcgctat attgcgtatt
      661 aaataaaacc acctcaatta cattaataaa tctaaacaaa catcatgcaa ataatttcca
      721 tggttatttc taggctattt aaattaaata aataaattat gagtcaatca taaataacaa
      781 cctttaatat aatttaacta ccaaatttat attcgttcac a
//
LOCUS       DAEEBB010000399          811 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEEBB010000399 DAEEBB010000000
VERSION     DAEEBB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.406"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..811
                     /locus_tag="KLF07_004574"
                     /pseudo
     CDS             1..811
                     /locus_tag="KLF07_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAEEBB010000400          795 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEEBB010000400 DAEEBB010000000
VERSION     DAEEBB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.407"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..741)
                     /locus_tag="KLF07_004575"
     CDS             complement(<1..741)
                     /locus_tag="KLF07_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBD9834336.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAEEBB010000401          794 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEEBB010000401 DAEEBB010000000
VERSION     DAEEBB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.408"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            106..507
                     /gene="spa15"
                     /locus_tag="KLF07_004576"
                     /gene_synonym="spaK"
     CDS             106..507
                     /gene="spa15"
                     /locus_tag="KLF07_004576"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBD9834337.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            511..>794
                     /locus_tag="KLF07_004577"
     CDS             511..>794
                     /locus_tag="KLF07_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="invasion protein InvC; necessary for efficient
                     entry of S.typhimurium into cultured epithelial cells;
                     probable catalytic subunit of a protein translocase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HBD9834338.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAV"
BASE COUNT          244 a          115 c          161 g          274 t
ORIGIN      
        1 agacttatag ataacagatt taagagtata ctcgttatat cgtatgctga gattgatgaa
       61 gcatatacca ttaatgtatt aaagactatt tagtgaggtt taaatatgag taacattaat
      121 ttagttcaat tagttagaga tagtcttttc acgattggtt gtcctccatc aattattaca
      181 gatttagaca gccattcagc gattaccatt tctttggata gtatgcctgc tattaatata
      241 gctcttgtca atgagcaggt tatgttatgg gctaattttg atgcaccaag tgatgtaaaa
      301 cttcagtcat ctgcttataa catattaaat ttaatgttaa tgaactttag ttatagcatt
      361 aatgagctgg tagagcttca tcgttctgat gagtacttac agttaagagt tgttattaaa
      421 gatgactacg ttcacgatgg gattgttttt gcagagattc tgcatgagtt ttatcagagg
      481 atggaaatct taaatgaggt cttataatca atgagctata caaaattgct cactcaatta
      541 tcttttccta atagaatctc ggggccaatc ttggaaacaa gtcttagcga tgtttcgatt
      601 ggtgagattt gtaacattca ggctggaatt gaaagtaatg aaattgttgc aagagctcag
      661 gttgtaggat ttcatgatga aaaaacaata ttaagcttga ttggaaattc tcgtggactt
      721 tcacggcaaa cgttgattaa gcccactgcc cagtttcttc atacgcaagt cggccgtgga
      781 ttattgggag cagt
//
LOCUS       DAEEBB010000402          792 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEEBB010000402 DAEEBB010000000
VERSION     DAEEBB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.409"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>792
                     /locus_tag="KLF07_004578"
     CDS             <1..>792
                     /locus_tag="KLF07_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HBD9834339.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          282 a          116 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc ca
//
LOCUS       DAEEBB010000403          788 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEEBB010000403 DAEEBB010000000
VERSION     DAEEBB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.410"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            211..>788
                     /locus_tag="KLF07_004579"
     CDS             211..>788
                     /locus_tag="KLF07_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HBD9834340.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAEEBB010000404          775 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEEBB010000404 DAEEBB010000000
VERSION     DAEEBB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.411"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..343
                     /locus_tag="KLF07_004580"
     CDS             <1..343
                     /locus_tag="KLF07_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBD9834341.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="KLF07_004581"
     CDS             complement(405..>775)
                     /locus_tag="KLF07_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBD9834342.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAEEBB010000405          769 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEEBB010000405 DAEEBB010000000
VERSION     DAEEBB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.412"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..343
                     /gene="spa40"
                     /locus_tag="KLF07_004582"
                     /gene_synonym="spaS"
     CDS             <1..343
                     /gene="spa40"
                     /locus_tag="KLF07_004582"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBD9834343.1"
                     /translation="SRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAP
                     APFISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRL
                     IVWLEQVENTH"
     gene            356..598
                     /locus_tag="KLF07_004583"
     CDS             356..598
                     /locus_tag="KLF07_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834344.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT          258 a          108 c          140 g          263 t
ORIGIN      
        1 gtcgagaagg cgtgagttgc atatcgagat tctttcagag cagactaaat ctgatatacg
       61 taattcaaaa ttagtggtaa tgaacccgac tcatattgca attggtattt attttaatcc
      121 agaaatagcg cctgcacctt ttatttctct cattgaaact aaccagtgtg ccttggctgt
      181 cagaaaatat gcaaatgaag ttggtatacc aactgtgcgt gatgtgaaat tagctagaaa
      241 actatataaa acacatacaa aatatagttt tgttgatttt gaacacttgg atgaagtcct
      301 acgtcttatt gtttggcttg agcaggttga aaacactcat taataagtaa aggagatgtg
      361 ttatatggga gttaatttct gtaataaaat aggtattgat cagtctgaat ttgaaataga
      421 atcttccatc attaactcca ttgctaatga ggtattgaac ccaatatctt tccttagcaa
      481 taaggatata ataaatgttt tactcagaaa gatttcatct gaatgtgacc tggttagaaa
      541 agacatttat cgttgtgctc tggagttggt cgttgaaaaa acacctgatg atttataatc
      601 attcataata actcttttgc tgatataaat atttttgttg aagatattta tgtagtcaca
      661 ggatcaattg tagaaaattg ttatgttttt gttaggaact gcactaaata atatatgata
      721 agacagcaaa aacgactgac cataatcctt cttttattag gagttgata
//
LOCUS       DAEEBB010000406          761 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEEBB010000406 DAEEBB010000000
VERSION     DAEEBB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.413"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>761
                     /gene="ipaB"
                     /locus_tag="KLF07_004584"
     CDS             <1..>761
                     /gene="ipaB"
                     /locus_tag="KLF07_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBD9834345.1"
                     /translation="EEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSF
                     SAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQSLQESR
                     KTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASLALAAVG
                     LALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKM
                     IGSILGAIAGALVLVAAVVLVATVGKQAAAKLAEN"
BASE COUNT          251 a          155 c          162 g          193 t
ORIGIN      
        1 gggaagaaat acaactcact atcaaaaaag acgcagcagt taaagacagg acattgattg
       61 agcagaaaac cctgtcaatt catagcaaac ttacagataa atcaatgcaa ctcgaaaaag
      121 aaatagactc tttttctgca ttttcaaaca cagcatctgc tgaacagcta tcaacccagc
      181 agaaatcatt aaccggactt gccagtgtta ctcaattgat ggcaaccttt attcaactag
      241 ttggaaaaaa taatgaagaa tctttaaaaa atgatctggc tctattccag tctctccaag
      301 aatcaagaaa aactgaaatg gagagaaaat ctgatgagta tgctgctgaa gtacgtaaag
      361 cagaagaact caacagagta atgggttgtg ttgggaaaat acttggggca cttttaacta
      421 tcgttagtgt tgttgcagca gctttttctg gaggagcctc tctagcactg gcagctgttg
      481 gtttagctct tatggttacg gatgctatag tacaagcagc gaccggcaat tccttcatgg
      541 aacaagccct gaatccgatc atgaaagcag tcattgaacc cttaatcaaa ctcctttcag
      601 atgcatttac aaaaatgctc gaaggcttgg gcgtcgactc gaaaaaagcc aaaatgattg
      661 gctctattct gggggcaatc gcaggcgctc ttgtcctggt tgcagcagta gttctcgtag
      721 ccactgttgg taaacaggca gcagcaaaac ttgcagagaa t
//
LOCUS       DAEEBB010000407          751 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEEBB010000407 DAEEBB010000000
VERSION     DAEEBB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.414"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            584..>751
                     /locus_tag="KLF07_004585"
     CDS             584..>751
                     /locus_tag="KLF07_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBD9834346.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAEEBB010000408          737 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEEBB010000408 DAEEBB010000000
VERSION     DAEEBB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.415"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..671
                     /gene="ospC1"
                     /locus_tag="KLF07_004586"
     CDS             <1..671
                     /gene="ospC1"
                     /locus_tag="KLF07_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBD9834347.1"
                     /translation="EVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIRKSED
                     QSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAVTASN
                     YEEINKQVTNKKIALQALFLSIANQKEDVALYILSNFEITRQDVISIKHELYDIEYLL
                     SAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYGATSD
                     NKYI"
BASE COUNT          280 a           96 c          136 g          225 t
ORIGIN      
        1 cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac
       61 caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta
      121 gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg
      181 atctggatag aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa
      241 gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag
      301 catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc
      361 tttttctttc gattgctaat caaaaagagg atgtcgcctt atatatatta tctaattttg
      421 agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac
      481 ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg
      541 atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa
      601 aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata
      661 aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg
      721 atatattgtt tattgtc
//
LOCUS       DAEEBB010000409          734 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEEBB010000409 DAEEBB010000000
VERSION     DAEEBB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.416"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(42..314)
                     /locus_tag="KLF07_004587"
     CDS             complement(42..314)
                     /locus_tag="KLF07_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834348.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            complement(397..734)
                     /locus_tag="KLF07_004588"
                     /pseudo
     CDS             complement(397..734)
                     /locus_tag="KLF07_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          156 a          180 c          193 g          205 t
ORIGIN      
        1 ggttggccgt gaacagacgt ttcagcggtc ggggctgaaa atcaccacca cttcgggtca
       61 tccgccttca tctccgcttc tgtttcgtat aaatcaaaac gacggcaggt atggcagaac
      121 gtgacgtatt cgtacggtga atccgggtac attttgtgga aggtttccca gaaacagacc
      181 gtgcaggagg gatgaccggc gataaagtct gtaaatacgg actgataagg gtgattattg
      241 gctctggcga cggctttcag aacctctttc accattctgg tgtggacttt ctggtgctcc
      301 aggttgtgtg acatgggaac tcattctgga tggttactct gaaagctcat attctgccac
      361 accccgattt gcagccgcca ggctgccgtg gttcttacgc cagaagggat ttgtaactgg
      421 tctggtcggc tccggcgtat cagtcagtgc caccgggaca ggcatgcatt cctgtatgtc
      481 atcatcgctc agtaagccgt cctcgtactg gcttttccat ttaaacagca ggttattatt
      541 gatatcgtgt tctctggcga tccgggcaac aacagcccca ggctgtaatg cctgcttagc
      601 cagacggacc ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc
      661 tctgatttga ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg
      721 agcgaatgaa actg
//
LOCUS       DAEEBB010000410          731 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEEBB010000410 DAEEBB010000000
VERSION     DAEEBB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.417"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            81..239
                     /locus_tag="KLF07_004589"
                     /pseudo
     CDS             81..239
                     /locus_tag="KLF07_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            344..535
                     /locus_tag="KLF07_004590"
     CDS             344..535
                     /locus_tag="KLF07_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBD9834349.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="KLF07_004591"
     CDS             complement(536..>731)
                     /locus_tag="KLF07_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBD9834350.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAEEBB010000411          730 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEEBB010000411 DAEEBB010000000
VERSION     DAEEBB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.418"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>730)
                     /locus_tag="KLF07_004592"
     CDS             complement(<1..>730)
                     /locus_tag="KLF07_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HBD9834351.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAF"
BASE COUNT          194 a          194 c          155 g          187 t
ORIGIN      
        1 aaaaaggcaa acagatccga agcgggataa tctttttggg gagaattgtt ccagttagca
       61 atatatcccg acagggggtt atacacctta gggttcattt caaaaggcaa tagccctttc
      121 cagtcccatt ttcccgtacc aggaacgggt aatcgcggat catggcctga ttgacgatct
      181 ggataagcac cagtatgaac ataaccaata ttgccgttta catcagcata gtaccagttg
      241 atggtcagtg cttgtttcgc tgcctgctgt gtccactcct gccaattttt ggccttcatc
      301 tgatgagtcc aggccagcaa agacgccacc tctttaccat cccatgcgcg ggatttagcg
      361 taagccgttt gtgtcgtctg gtcagtttgg agaatgttgc catgcaccgt acgccagaca
      421 gtaaaggtct ctgcctgacc atttttcacc gtaatggttt cctcacggct taacattttc
      481 acccacttac cattatgcaa gtagtagcct ggtttctctg ccaacagccg ttcagcaaaa
      541 atatcgacat catcgccgaa acctgccgtt gatccccagg aaatcacacc attatgacca
      601 aaaaccagcc caggataggc aaatggtgta ttgccagtga catcataacc agcaccgtgc
      661 agaccaatac cataagtata cgcaggcgca taccagccaa actgcggacc atttaccatg
      721 attgctttcg
//
LOCUS       DAEEBB010000412          730 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEEBB010000412 DAEEBB010000000
VERSION     DAEEBB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.419"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(162..>730)
                     /locus_tag="KLF07_004593"
     CDS             complement(162..>730)
                     /locus_tag="KLF07_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD9834352.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga
//
LOCUS       DAEEBB010000413          726 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEEBB010000413 DAEEBB010000000
VERSION     DAEEBB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.420"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            35..726
                     /locus_tag="KLF07_004594"
                     /pseudo
     CDS             35..726
                     /locus_tag="KLF07_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAEEBB010000414          710 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEEBB010000414 DAEEBB010000000
VERSION     DAEEBB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.421"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAEEBB010000415          706 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEEBB010000415 DAEEBB010000000
VERSION     DAEEBB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.422"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>706)
                     /gene="tssI"
                     /locus_tag="KLF07_004595"
     CDS             complement(<1..>706)
                     /gene="tssI"
                     /locus_tag="KLF07_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBD9834353.1"
                     /translation="SLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSAQIRPS
                     SVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWR
                     NNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGRRGSGT
                     TLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRY
                     NPSNQDSSCWIRVAQAW"
BASE COUNT          123 a          226 c          199 g          158 t
ORIGIN      
        1 cccacgcctg tgccacacgg atccagcatg aactgtcctg gtttgacggg ttatagcggt
       61 cccagttaaa tttcacccgc acgcggccat gttcatcaca gaagatttcc tcgcctgccg
      121 gtcccgtcac gacggcgctc tgcgggccat ccaccagcgg tttcatcaac ggctgtggcc
      181 gccatgttct gtctgccggg attaccgcaa aatggttctc cagcgtggtg cctgaacccc
      241 tgcgtcccgg taccgcctgc ggctgttcgc cgtgaagatc gctcgccacc acctgccatt
      301 cccggttcag gttcgcctgc ggatgccccg tcagcacaat tcgccgtccc ggccatatct
      361 ccggcgaacg gcttgttccg cgcgccactt ctgcgttgtt tcgccagccg tccatctgcc
      421 agcgggcaaa gttctgcccg tgggcaccct tgaaacgtcc ggggtagtca tacacttcat
      481 actgcgtgcg ctggtaatcc tggtactggc cttcctgatc aaaacgccct ggccagccgg
      541 gacgtttaaa ggtgtagtct ttggtcacta cggaagaagg acggatttgt gcgctgtagc
      601 gaaactggct gatgcagagg gtacttacct cggtacgggt gttcgggttc caggggatct
      661 caaaggactc cggcagataa cgcacggtgt cgcacaggac caggct
//
LOCUS       DAEEBB010000416          698 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEEBB010000416 DAEEBB010000000
VERSION     DAEEBB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.423"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>698)
                     /gene="ipaH7.8"
                     /locus_tag="KLF07_004596"
     CDS             complement(<1..>698)
                     /gene="ipaH7.8"
                     /locus_tag="KLF07_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBD9834354.1"
                     /translation="NNTHSSVSCSPSINSNSTSNEYYLRILTEWEKNSSPGEERGIAF
                     NRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNADN
                     NRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLKFLHV
                     YYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPESFSQ
                     LDSSYRINISGNPL"
BASE COUNT          223 a          100 c          145 g          230 t
ORIGIN      
        1 caatggattc cctgaaatat taatcctgta acttgaatct aattgtgaaa aactctccgg
       61 aagagtggtt atctgattaa aatgaaaata atattccttt tgtctgatat tgtttctatc
      121 agaaaattga ggaaaagtaa ccagattatt gcgctgagca cagagaattt ccagtttatc
      181 cggtaagtcg ggtaatgttg tcagcctgtt ataataaaca tgtaaaaatt tcaatttttc
      241 gggaagagcc ggtaagttat atagcctgtt attttcaaca aataatgatg ttaaatggtt
      301 tggcaaaaca ggaaggtttt ccagttgatt agaacgaaca cttaaagttg ttaatgactc
      361 aggaagttct ggtatcacag aaagcctgtt attatcagca ttaagttctt tgagaaatgc
      421 aggcagcttt ggcaatgatg ttaatttatt attttctaca atcaaagcag aaatatgctt
      481 tggtaattcg ggaagagacg tcaaatttag gtctgataaa tttaatactg cttcttgatt
      541 ctgaaagcac tgggagagtc tgttaaaagc aatgcctcgc tcttccccgg gagaagagtt
      601 cttttcccat tcagtcagga ttctcagata atattcatta ctggttgagt ttgagttaat
      661 agagggggag caagaaactg atgagtgtgt attattta
//
LOCUS       DAEEBB010000417          695 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEEBB010000417 DAEEBB010000000
VERSION     DAEEBB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.424"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>695
                     /gene="tuf"
                     /locus_tag="KLF07_004597"
     CDS             <1..>695
                     /gene="tuf"
                     /locus_tag="KLF07_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD9834355.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAEEBB010000418          694 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEEBB010000418 DAEEBB010000000
VERSION     DAEEBB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.425"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>694
                     /locus_tag="KLF07_004598"
     CDS             <1..>694
                     /locus_tag="KLF07_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9834356.1"
                     /translation="SRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAI
                     NAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLR
                     VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCY
                     RANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLA
                     EAFEHYNEWHPHS"
BASE COUNT          172 a          166 c          214 g          142 t
ORIGIN      
        1 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
       61 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      121 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      181 tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag tggctgtgaa
      241 agaaagtaat cagcgatggt gctctgacgg gttcgagttc cgctgtgata acggagaaaa
      301 actgcgagtc acgttcgcgc tggactgctg tgaccgtgag gcactgcact gggcggtcac
      361 tacgggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      421 cttcggcaac gagcttccgg cgtctccagt agagtggctg acggataatg gttcatgcta
      481 ccgggctaat gaaacacggc agtttgcccg gatgttgggg cttgaaccga agagcacggc
      541 ggtgcggagt ccggagagta acggcatagc agagagcttc gtgaaaacga taaagcgtga
      601 ctacatcagt gtcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
      661 gttcgagcat tataacgaat ggcatccgca tagt
//
LOCUS       DAEEBB010000419          693 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEEBB010000419 DAEEBB010000000
VERSION     DAEEBB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.426"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..598
                     /gene="spa32"
                     /locus_tag="KLF07_004599"
     CDS             <1..598
                     /gene="spa32"
                     /locus_tag="KLF07_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBD9834357.1"
                     /translation="ENILIPDQDIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSS
                     VNPDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAKSFELPVMNKNTADITP
                     SMSLQEKSIVENDKNVFQKNSEMTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKL
                     DLILKQDAEGNYRFDSSQHNKGNKNNSTGYNEQSEEEC"
BASE COUNT          272 a           91 c          127 g          203 t
ORIGIN      
        1 agaaaatatt ttgataccag atcaagatat aaaagcaagg gaaaaaataa atatcggcga
       61 tatgaggggg atcttttcat ataataagag tggaaatgca gacaagaact tcgaaagaag
      121 tcatacttct tctgtaaacc ctgataatct gctagaatct gataatagaa atggtcaaat
      181 tggtttaaaa aatcatagct tgtctattga taagaatatt gctgacatca tttctttact
      241 aaatggaagt gttgctaaat catttgagct gcctgtaatg aataaaaata ctgcagacat
      301 aaccccatcc atgtcattgc aagaaaaatc aatagttgaa aatgataaaa atgtttttca
      361 aaaaaatagt gaaatgactt accactttaa acagtggggg gctggacatt ctgttagtat
      421 ttcagtggag tctggttctt ttgttctaaa accgtcagat caatttgtag gaaataaact
      481 tgacttaatt ttgaaacaag atgctgaggg taattataga tttgatagca gtcaacataa
      541 taaggggaat aaaaataata gtacaggata taatgaacag agtgaagaag aatgctaaga
      601 attaaacatt ttgacgctaa cgaaaaacta cagattttat atgcaaagca actctgcgag
      661 cgtttttcga ttcagacatt caaaaataaa ttt
//
LOCUS       DAEEBB010000420          691 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEEBB010000420 DAEEBB010000000
VERSION     DAEEBB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.427"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            39..>691
                     /gene="mxiJ"
                     /locus_tag="KLF07_004600"
     CDS             39..>691
                     /gene="mxiJ"
                     /locus_tag="KLF07_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBD9834358.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYLFLG
                     "
BASE COUNT          230 a           95 c          135 g          231 t
ORIGIN      
        1 gtttcggctg ttgaaacttt attaaagtct taatttatat gattaggtat aaaggtttta
       61 ttttattctt gttgctgatg ttgattggat gtgagcaacg tgaagagtta atttctaatt
      121 tatctcaaag acaggcaaat gaaataatat ctgtgctaga acgccataat attactgcta
      181 gaaaagttga tggaggtaaa caggggatct cggtacaagt cgaaaagggg acatttgcat
      241 cggcagttga tttgatgcgc atgtacgatt tgccaaatcc ggagagagtt gatatctcac
      301 aaatgtttcc tacagattca ttagtgtctt ctccaagagc tgaaaaggcc cgtttatata
      361 gtgctattga gcaacggctg gaacagtctt tagtttctat tggtggtgtt atttcggcaa
      421 aaatacatgt tagctatgat cttgaagaaa aaaatatatc ttcaaaaccg atgcatatat
      481 cagtaatcgc tatatatgac tcaccgaaag agtctgaact attagttagt aatattaagc
      541 gatttttgaa aaacaccttt tctgatgtta agtatgaaaa tatatctgtc atattaactc
      601 cgaaagaaga atatgtttat acaaatgtac aacctgttaa ggaaattaaa tcggaatttt
      661 taacaaatga agtaatatat ttatttctcg g
//
LOCUS       DAEEBB010000421          683 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEEBB010000421 DAEEBB010000000
VERSION     DAEEBB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.428"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..560
                     /locus_tag="KLF07_004601"
     CDS             <1..560
                     /locus_tag="KLF07_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBD9834359.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          244 a          121 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acg
//
LOCUS       DAEEBB010000422          677 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEEBB010000422 DAEEBB010000000
VERSION     DAEEBB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.429"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..361
                     /locus_tag="KLF07_004602"
                     /pseudo
     CDS             1..361
                     /locus_tag="KLF07_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAEEBB010000423          666 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEEBB010000423 DAEEBB010000000
VERSION     DAEEBB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.430"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..152)
                     /locus_tag="KLF07_004603"
                     /pseudo
     CDS             complement(1..152)
                     /locus_tag="KLF07_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(267..>666)
                     /locus_tag="KLF07_004604"
     CDS             complement(267..>666)
                     /locus_tag="KLF07_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD9834360.1"
                     /translation="VVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRW
                     PGWLERVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVD
                     AYIRWYNERRIKLSLGAVSPKMYRQQCGLE"
BASE COUNT          150 a          183 c          165 g          168 t
ORIGIN      
        1 acctcgatat agccatccca ggagacatga cggatatcga agtccgtacc cagcagcggc
       61 tgcagatgct cctgttcctg cgtgaagcgc tgttccggtg tctgcctgaa ctggcgaagt
      121 tcccgcctgt cggcaacgtc agccatccac tgcgcagcat gagagtttcc tttgtggtgt
      181 aggaacctta aggaaacaac atgttgggtg gagcggacaa tccaaatggt gaagggggga
      241 tgcggacgat ttcctggact gctttatcat tccagcccgc attgttggcg gtacattttg
      301 gggctgacgg cacccagcga taattttata cgccgctcgt tataccatct gatgtaagca
      361 tccacttgct gcatgaactt ttctggcgtg atgcccgacc atttacgccc ataatacatt
      421 tccgttttca gtctgccgaa aaagccttcg catgcagcat tatcaggtga acatccttta
      481 cgggacattg agcgaataag acctgctgca ttcactcttt ccagccagcc tggccagcga
      541 taatgcccac ctctgtcact gtgtatcacc ggtcgttcgc cagcatttaa cgtttcgaca
      601 gcgctatcca gcatagtgtt gaccagttca gcatcagggc gtgtactgag agaccagctc
      661 acaact
//
LOCUS       DAEEBB010000424          642 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEEBB010000424 DAEEBB010000000
VERSION     DAEEBB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.431"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            200..307
                     /locus_tag="KLF07_004605"
     CDS             200..307
                     /locus_tag="KLF07_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD9834361.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT          170 a          154 c          180 g          138 t
ORIGIN      
        1 cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc
       61 cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac
      121 aacagaacgg gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc
      181 attacaacaa ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct
      241 ggcagcaccg atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg
      301 gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc
      361 gcaggggtaa cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca
      421 acccgagctg gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa
      481 ccggaatgca ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta
      541 gtatccacat caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg
      601 gtggcattgg ttgctggaaa gagaaaaccc ccgcacgttg ag
//
LOCUS       DAEEBB010000425          641 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEEBB010000425 DAEEBB010000000
VERSION     DAEEBB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.432"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="KLF07_004606"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="KLF07_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBD9834362.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAEEBB010000426          606 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEEBB010000426 DAEEBB010000000
VERSION     DAEEBB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.433"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>606
                     /locus_tag="KLF07_004607"
     CDS             <1..>606
                     /locus_tag="KLF07_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphatase"
                     /protein_id="HBD9834363.1"
                     /translation="WGPVNKNISHHGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICC
                     ASTRESDHIANMWLSKVVDDEGKEIFSGIRHGVISAYGLKKNSSERAVAARNKAEELV
                     SAALYSRPELLSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRG
                     EPTKLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGWR"
BASE COUNT          204 a           80 c          155 g          167 t
ORIGIN      
        1 tggggaccgg tcaacaagaa tattagtcat catgggaaaa attatggttt tcaattaaca
       61 cctgcctctc atatgaagat agggaataaa aatatatttg tcaaagagta taatggaaaa
      121 ggaatttgtt gtgcttctac gagagagagt gatcatatcg cgaatatgtg gctttcaaaa
      181 gtggtggatg acgaggggaa ggaaattttt tcgggtatca gacatggagt tatatcagcc
      241 tatggattga aaaaaaactc atctgaaaga gctgttgctg ctcgtaataa agctgaggag
      301 ttagtaagcg cagcattata tagcagacca gagttattat cacaggcttt gtctggtaaa
      361 acagtagatt taaagattgt ttcgacttct cttctgacgc cgaccagttt aaccggggga
      421 gaggagagta tgttaaaaga tcaggtgaac gcattaaaag ggttaaactc taaaaggggg
      481 gagccaacga aattattaat tcggaatagt gatggtcttc tgaaagaagt aagcgttaat
      541 ctaaaagtgg tgacatttaa ctttggtgta aatgaattag cgctaaaaat gggcttaggc
      601 tggagg
//
LOCUS       DAEEBB010000427          597 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.434, whole genome shotgun sequence.
ACCESSION   DAEEBB010000427 DAEEBB010000000
VERSION     DAEEBB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.434"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="KLF07_004608"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="KLF07_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBD9834364.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          130 a          162 c          171 g          134 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctggggg
//
LOCUS       DAEEBB010000428          593 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEEBB010000428 DAEEBB010000000
VERSION     DAEEBB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.435"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="KLF07_004609"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="KLF07_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBD9834365.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAEEBB010000429          593 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEEBB010000429 DAEEBB010000000
VERSION     DAEEBB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.436"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..60)
                     /locus_tag="KLF07_004610"
     CDS             complement(<1..60)
                     /locus_tag="KLF07_004610"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834366.1"
                     /translation="MASVSISYPSCSATDGVVRN"
     gene            116..593
                     /locus_tag="KLF07_004611"
                     /pseudo
     CDS             116..593
                     /locus_tag="KLF07_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          146 a          132 c          134 g          181 t
ORIGIN      
        1 gttacgcacc accccgtcag tagctgaaca ggagggatag ctgatagaaa cagaagccac
       61 tggagcgcct caaaaacacc atcatacact aaatcagtaa gttggcagca tcaccgccgt
      121 tgtctacaca tttattctct cggtgctgat ttaccgggaa gtaaaatggc gccatttacc
      181 caaactgatt ctggagtcgg ttgttaccac ttcgattgta ttactgttga tcggtttttc
      241 cgtgggcatg tcctgggcaa tgactaatgc tgatattcct tatatgatca gcgacgcatt
      301 gatggggatt tctgataacc cactgatcat attgctgctg attaatatcg tcttattaat
      361 tgtcggcatc tttatggaca tgacaccggc tgtactaatc tttacaccga tcttcttgcc
      421 gattgcacaa gagctgggaa tggacccagt acatttcggt attatgatgg tggccaacct
      481 ttgtattggg ctgttaacac cgccggtggg tagtgcgcta tttgttggtt gttctatctc
      541 gggtgtgaag attcaacatt taatcaaacc attgctgcca ttttatgcta gca
//
LOCUS       DAEEBB010000430          578 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.437, whole genome shotgun sequence.
ACCESSION   DAEEBB010000430 DAEEBB010000000
VERSION     DAEEBB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.437"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
BASE COUNT          173 a          139 c          119 g          147 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcctg atctctgtcc cggtatttta gcccgaggta
      301 tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca ttcgctaaca
      361 gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg ttcataagcg
      421 tattatcagg attgtctgga aaattcccga tactttggac tattactcct gaacaattcg
      481 aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag gtaagcgtaa
      541 accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAEEBB010000431          575 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEEBB010000431 DAEEBB010000000
VERSION     DAEEBB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.438"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAEEBB010000432          574 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEEBB010000432 DAEEBB010000000
VERSION     DAEEBB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.439"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..516)
                     /locus_tag="KLF07_004612"
     CDS             complement(<1..516)
                     /locus_tag="KLF07_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD9834367.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          160 a          137 c          115 g          162 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttac
//
LOCUS       DAEEBB010000433          567 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.440, whole genome shotgun sequence.
ACCESSION   DAEEBB010000433 DAEEBB010000000
VERSION     DAEEBB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.440"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..396)
                     /locus_tag="KLF07_004613"
     CDS             complement(<1..396)
                     /locus_tag="KLF07_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD9834368.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          165 a          144 c           92 g          166 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcata
//
LOCUS       DAEEBB010000434          552 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.441, whole genome shotgun sequence.
ACCESSION   DAEEBB010000434 DAEEBB010000000
VERSION     DAEEBB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.441"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..552
                     /locus_tag="KLF07_004614"
                     /pseudo
     CDS             1..552
                     /locus_tag="KLF07_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          196 a          120 c           84 g          152 t
ORIGIN      
        1 gattatccga cttattaaaa atgcctgttt aaaattatat aatttagcac ataccgcaac
       61 caactcccac cttaaaccaa ctggtccgga taattcagat gttctcctgt tcaaaaagct
      121 attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa
      181 acagtcgctt tcaaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa
      241 cttctgggat aaaaatcgtc aattaaccac agatgaaata gcttgctatc ttcagaagat
      301 cgccgctaat gcaaaaaata ctcaagtcaa ttatcctact ggtctctacg tcccctactc
      361 caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc
      421 gaatgaagtc cagttattcc ccataaatac tggcggacac tggatattag tttcgctaca
      481 gaaaatagta aataaaaaaa taataaacta caaataaaat gcgtcatatt caactcattg
      541 cgtgcactag gc
//
LOCUS       DAEEBB010000435          551 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.442, whole genome shotgun sequence.
ACCESSION   DAEEBB010000435 DAEEBB010000000
VERSION     DAEEBB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.442"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>551
                     /gene="feaR"
                     /locus_tag="KLF07_004615"
     CDS             <1..>551
                     /gene="feaR"
                     /locus_tag="KLF07_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBD9834369.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          141 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc a
//
LOCUS       DAEEBB010000436          549 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEEBB010000436 DAEEBB010000000
VERSION     DAEEBB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.443"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>549
                     /locus_tag="KLF07_004616"
     CDS             <1..>549
                     /locus_tag="KLF07_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBD9834370.1"
                     /translation="VVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAP
                     LVKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFDSQT
                     GRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYKASR
                     EPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          113 a          150 c          166 g          120 t
ORIGIN      
        1 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
       61 cgaatgggtg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgccggagct
      121 gcactggccc cgctggtgaa actgctacgc catgagcttc tgacacggga tgttattcat
      181 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
      241 tggctgtgtg cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg ttttgactcg
      301 cagaccggac gggccctcag ataccctgag acctggctgc agtgctggtg tggcggtacg
      361 ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatcaccc cggcatcaca
      421 tcagcctgtt gctggagtca cgcagggcgc ggcttcgcaa atctgtataa ggccagcagg
      481 gagccccgtg caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat tgagaaactc
      541 atccgtgag
//
LOCUS       DAEEBB010000437          549 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.444, whole genome shotgun sequence.
ACCESSION   DAEEBB010000437 DAEEBB010000000
VERSION     DAEEBB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.444"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..182)
                     /gene="mxiI"
                     /locus_tag="KLF07_004617"
     CDS             complement(<1..182)
                     /gene="mxiI"
                     /locus_tag="KLF07_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBD9834371.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNP"
     gene            complement(195..446)
                     /gene="mxiH"
                     /locus_tag="KLF07_004618"
     CDS             complement(195..446)
                     /gene="mxiH"
                     /locus_tag="KLF07_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBD9834372.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(454..>549)
                     /locus_tag="KLF07_004619"
     CDS             complement(454..>549)
                     /locus_tag="KLF07_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834373.1"
                     /translation="DDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
BASE COUNT          161 a          103 c           76 g          209 t
ORIGIN      
        1 tctgggttta atgattctgg attgcttacc atctccatta tttgtgatac ttgaatatct
       61 gtatccatct taatatcact atatttagcc gacacgacat cttccagact tgatatctct
      121 tgagattgaa aatcactggc tttgataata tcaacctgat tgactggata aatgtaattc
      181 atgaatgctc cctattatct gaagttttga ataattgcag catcaacatc cttaatcact
      241 ttcactgtat tggattgcgc gttcctatat aatgtatatt cagataattt actttggtat
      301 tcagccagca actgtggatt cgaaggattt ttagctaatt tatctagtgc caatgttagt
      361 tcaccttgta atgtttgagt tccatcatca aaagtttcag ataatgaact cagtgtccaa
      421 tcatcattcg gtactgtaac actcatttta tcctcactta tttttatcta aaaaaaacca
      481 gtgtttatca ttcaacatta cataactgtc tttgctgtta agatatgatt tacctttaaa
      541 atcatcatc
//
LOCUS       DAEEBB010000438          545 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.445, whole genome shotgun sequence.
ACCESSION   DAEEBB010000438 DAEEBB010000000
VERSION     DAEEBB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.445"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(112..174)
                     /locus_tag="KLF07_004620"
                     /pseudo
     CDS             complement(112..174)
                     /locus_tag="KLF07_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          201 a           98 c           90 g          156 t
ORIGIN      
        1 caaccgactg aaggcgctgg ttgcgaaact gcagcgtatg tagttcggta aaagctcaga
       61 aaaacttcgc gcaaaaaccg aacggcagat tccattttca cgggcgatct acgccacaca
      121 ggctctgggt tgcagtgact gctccattaa ggcgattttg aactcataag ggaagtaaca
      181 aaaaaactgc aaaagtacaa taattatata tggccaaact aacctctatt aaaaaaacat
      241 cagtttttca cacttagttg agcttcatga aaaaatcatc gaggatttca ttgcagaata
      301 cacattccac taactggaag cactccataa gaggaatcag agaaagtatc gtttttttat
      361 agtaaaattc attgctgttc aataaaattg atatagttat taaattatga aaataatttc
      421 acaaaaaaga ttaacttgag ttcttaagca tcggatttta gatattttga aatatctggc
      481 atcacataac atcaaaagta tataacaccc ttatctaatt acatgcagta tattgtatat
      541 atact
//
LOCUS       DAEEBB010000439          543 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.446, whole genome shotgun sequence.
ACCESSION   DAEEBB010000439 DAEEBB010000000
VERSION     DAEEBB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.446"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..438
                     /locus_tag="KLF07_004621"
     CDS             <1..438
                     /locus_tag="KLF07_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD9834374.1"
                     /translation="TDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLILHAPSFVSVS
                     EKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHKIKLTEAIRKL
                     RRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          184 a           96 c          100 g          163 t
ORIGIN      
        1 actgataata aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg
       61 ctttcatgta ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt
      121 gtttctgtgt ctgaaaaaat cgccagaatt gtgatggcaa attactcccg taactggagt
      181 aattccgagt tggccagtgc ggttttaatg agtgaatctt ctttaaaaag aagaatgtac
      241 aaagaggtcg gttctataag tacatttgta cataaaataa agcttacaga agctatcagg
      301 aaattacgaa ggacaaatac tcccatttct gtcatatcat ctgagttagg ttacagttca
      361 ccatcgtatt tttctaaagt tttctttaaa tatttaaaga cataccctca aaacataagg
      421 aagaaaaatg gtcggtaatg tcaccaatta ggaggacttg cagagccgct tttttacgat
      481 actcaccccg taagtaagcg ataattggta acttaaacgc ccatctgaca tagatggaca
      541 cct
//
LOCUS       DAEEBB010000440          520 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.447, whole genome shotgun sequence.
ACCESSION   DAEEBB010000440 DAEEBB010000000
VERSION     DAEEBB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.447"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>520
                     /locus_tag="KLF07_004622"
     CDS             <1..>520
                     /locus_tag="KLF07_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD9834375.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAEEBB010000441          517 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.448, whole genome shotgun sequence.
ACCESSION   DAEEBB010000441 DAEEBB010000000
VERSION     DAEEBB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.448"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>517)
                     /locus_tag="KLF07_004623"
     rRNA            complement(<1..>517)
                     /locus_tag="KLF07_004623"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a          170 c          110 g          140 t
ORIGIN      
        1 gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc gagttctctc
       61 aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga tttgatgtta
      121 cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca ccgtagtgcc
      181 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
      241 aaaccgggac aaccgtcgcc cggccaacat agccttctcc gtcccccctt cgcagtaaca
      301 ccaagtacag gaatattaac ctgtttccca tcgactacgc ctttcggcct cgccttaggg
      361 gtcgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc ggcgagcggg
      421 cttttcaccc gctttatcgt tacttatgtc agcattcgca cttctgatac ctccagcatg
      481 cctcacagca caccttcgca ggcttacaga acgctcc
//
LOCUS       DAEEBB010000442          507 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.449, whole genome shotgun sequence.
ACCESSION   DAEEBB010000442 DAEEBB010000000
VERSION     DAEEBB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.449"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..188)
                     /locus_tag="KLF07_004624"
     CDS             complement(<1..188)
                     /locus_tag="KLF07_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9834376.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="KLF07_004625"
                     /pseudo
     CDS             242..445
                     /locus_tag="KLF07_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAEEBB010000443          503 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.450, whole genome shotgun sequence.
ACCESSION   DAEEBB010000443 DAEEBB010000000
VERSION     DAEEBB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.450"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            223..>503
                     /locus_tag="KLF07_004626"
     CDS             223..>503
                     /locus_tag="KLF07_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD9834377.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          106 g          129 t
ORIGIN      
        1 cttaaagatt ttaacgatcg cttattgtaa aaataatagc aaagaaaatt gtaagataca
       61 gaccgtaaat aaaaacatct tccttggtca ggaaagaaat cttgaaagca tatctggcta
      121 cgacaaaatc gttgtcaaat ataataactg gatcaccaaa gataatggcg agtttgaact
      181 ggcggtccat taattatcat tgtaataaac gaaggaatag taatgaaaaa ggtatttgca
      241 aaatctcttc tggtcgcagc gatgttttct gttgcaggct ccgcattggc agtacaaaag
      301 gatattactg taacggccaa cgttgatgcc gctctggata tgacacagac cgataacacc
      361 gcgttgccga aagcagtaga aatgcaatat ctgccggggc agggtctgca atcttaccag
      421 ttgatgacca aaatctggtc taacgacgtt accaaagatg taaaaatgca gttagtttcc
      481 ccggcgcaac tggttcaaag cct
//
LOCUS       DAEEBB010000444          501 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.451, whole genome shotgun sequence.
ACCESSION   DAEEBB010000444 DAEEBB010000000
VERSION     DAEEBB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.451"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            30..206
                     /locus_tag="KLF07_004627"
     CDS             30..206
                     /locus_tag="KLF07_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834378.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          178 a           59 c          111 g          153 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagatc atcagaagtt cactttttgt actaaataat tcgcatttta tgtttaaaaa
      481 ttgagatatt ccttattacc t
//
LOCUS       DAEEBB010000445          496 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.452, whole genome shotgun sequence.
ACCESSION   DAEEBB010000445 DAEEBB010000000
VERSION     DAEEBB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.452"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..159
                     /locus_tag="KLF07_004628"
     CDS             <1..159
                     /locus_tag="KLF07_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD9834379.1"
                     /translation="RDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIR
                     LFYQRLRN"
     gene            298..453
                     /locus_tag="KLF07_004629"
                     /pseudo
     CDS             298..453
                     /locus_tag="KLF07_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          142 a          103 c          120 g          131 t
ORIGIN      
        1 cgtgatgcgt actgcaatcg taatgtcgtt gaacggtgct ttgggcggct gaaagaatat
       61 cgccgcatcg ccacgcgtta cgacaaaacg gcgagaaatt acctggcgat ggtgaaactg
      121 ggctgcatcc gactctttta tcaacgctta cgtaattaag ggacacagcc tggcactctg
      181 cgagtaaact tgctggtaaa ctattattga cagttggttt tgttttgaga ctgtaattta
      241 aattgtgtaa ttgcctgttt ttgatatgtt cactccaaca acggagacag gtaaattatg
      301 gacgaaaaga agcttaaagc ccttgcggct gaactggcta aaggcctcaa aaccgaggcc
      361 gacctcaatc agttttcccg tatgcagacg aaattaactg tcgaaacggt gcttaatgcg
      421 gaactaactg accatctcgg gcatgaaaaa agctacatcc ggtagtaaac attatcagtg
      481 ggttatgcga tatgat
//
LOCUS       DAEEBB010000446          487 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.453, whole genome shotgun sequence.
ACCESSION   DAEEBB010000446 DAEEBB010000000
VERSION     DAEEBB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.453"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(399..>487)
                     /locus_tag="KLF07_004630"
     CDS             complement(399..>487)
                     /locus_tag="KLF07_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBD9834380.1"
                     /translation="DNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          126 a          111 c          103 g          147 t
ORIGIN      
        1 gaaccgtttt gacggcttcg gctttaaact ccgtagagta acgttgggtt tttctgctca
       61 ttattggctc cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta
      121 cctcactccc gtaaagacgg gagtcaagtt cccgctccga cataactgcg tgcaatggat
      181 tttgaaggaa ccgtcagccc attccccgga ggcaaacatg ggctggcaaa agtgtagcgg
      241 tattaggcac agctatttag gaaagaggga ccatctccat ctcattaaat ggaccacgcc
      301 agacaattca ctgtcatgct gatgctgcac tgtggggaca gaatcactca tgatgacttc
      361 gataatcgac gacattatta tttggccgag cttttaggtc atatctcaaa tcgtttgcct
      421 aatattgctc cattttttaa taaaaaatca atcatttttg aatctttgct tttcattgca
      481 ttgtcaa
//
LOCUS       DAEEBB010000447          479 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.455, whole genome shotgun sequence.
ACCESSION   DAEEBB010000447 DAEEBB010000000
VERSION     DAEEBB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.455"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            1..479
                     /locus_tag="KLF07_004631"
                     /pseudo
     CDS             1..479
                     /locus_tag="KLF07_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          121 a          115 c          160 g           83 t
ORIGIN      
        1 tggcagaccg tcaggcccgg atgttcatca gttacagcga agtcagcata aaaaatcccg
       61 acaacagcgg ccaggcgctc ccgctcacgt atgtctgttg ccgggagcag gcagaggacg
      121 gtgcctgctg gcatctgctg acgtcaggaa aagcggcgag tgccgccgat gcacgacgta
      181 ttgtcagcca ttacgagcga cgctggctga cagaggaata ccacaaggcg tggaaaagtg
      241 gtggtacatg gaatcgctgc gaatgcagac ccgggataac cttgagcgca tggtggttat
      301 ccaggcgttt atagcggtac gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac
      361 acaaaatgac agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa
      421 gctggaagga aagccgcttc cggtgcaggc accgaccctg aaatgggcct ggggcgatg
//
LOCUS       DAEEBB010000448          476 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.456, whole genome shotgun sequence.
ACCESSION   DAEEBB010000448 DAEEBB010000000
VERSION     DAEEBB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.456"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>476
                     /locus_tag="KLF07_004632"
     rRNA            <1..>476
                     /locus_tag="KLF07_004632"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAEEBB010000449          472 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.457, whole genome shotgun sequence.
ACCESSION   DAEEBB010000449 DAEEBB010000000
VERSION     DAEEBB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.457"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            89..451
                     /locus_tag="KLF07_004633"
     CDS             89..451
                     /locus_tag="KLF07_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBD9834381.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAEEBB010000450          457 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.458, whole genome shotgun sequence.
ACCESSION   DAEEBB010000450 DAEEBB010000000
VERSION     DAEEBB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.458"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..386
                     /locus_tag="KLF07_004634"
     CDS             <1..386
                     /locus_tag="KLF07_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD9834382.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          123 g          105 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcactg
//
LOCUS       DAEEBB010000451          455 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.459, whole genome shotgun sequence.
ACCESSION   DAEEBB010000451 DAEEBB010000000
VERSION     DAEEBB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.459"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            133..>455
                     /locus_tag="KLF07_004635"
     CDS             133..>455
                     /locus_tag="KLF07_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HBD9834383.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDI"
BASE COUNT          182 a           80 c           63 g          130 t
ORIGIN      
        1 atattatgta caacaaaaga aaatttgatt atgttaacag gctcaagttt ctaaatttat
       61 taattgaata tattatatat caaatacatt ttacatcccc acaatcacca cgtaatggag
      121 agttaataaa gtatgatcct caaaattagc aatttcattg acgcaagcaa tacaaaaggg
      181 cctatacgcg ttgaagatac agagcatgga cctatattgg tagcacagaa attcaaccta
      241 aaggatctct ttttcagaac attaagcact attaatgcaa agataaattc acagatatta
      301 aatgaacaac taaaaaatta tcgactagcg aatcaaaaat ccttattgct tttcctcaag
      361 acattagcta gcgaaaaatc tgcagaaagt gcgtttgccg cctatgaagc agtcaaaaat
      421 tcaattcaac attcttttac aggtaaagac atcaa
//
LOCUS       DAEEBB010000452          453 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.460, whole genome shotgun sequence.
ACCESSION   DAEEBB010000452 DAEEBB010000000
VERSION     DAEEBB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.460"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            9..>453
                     /gene="ospC4"
                     /locus_tag="KLF07_004636"
     CDS             9..>453
                     /gene="ospC4"
                     /locus_tag="KLF07_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBD9834384.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSN"
BASE COUNT          162 a           83 c           72 g          136 t
ORIGIN      
        1 ggggggggat gaaaatacct gaagcagtaa atcatattaa tgttcagaat aatattgatc
       61 ttgttgatgg aaaaacgaat ccgaataaag caacaaaagc attacaaaaa aatatattac
      121 gcgtaacaaa ctcatcttct tctggcataa gtgaaaaaca tttagatcac tgtgctaata
      181 ctgtgaaaaa ctttttaagg aagtctatag cagcgcaaag ttatagcaaa atgttttctc
      241 aagggaccag tttcaaatca ctaaatcttt ctattgaagc gccatctgga gcacggtcat
      301 catttcggtc acttgagcat ctcgataagg tgtccagaca ctatatctct gagataatac
      361 aaaaagttca tcctctttct tcagacgaac gtcacttact ttctattatt attaattcta
      421 attttaactt cagacatcaa agtaactcta atc
//
LOCUS       DAEEBB010000453          451 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.461, whole genome shotgun sequence.
ACCESSION   DAEEBB010000453 DAEEBB010000000
VERSION     DAEEBB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.461"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..66
                     /locus_tag="KLF07_004637"
     CDS             <1..66
                     /locus_tag="KLF07_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBD9834385.1"
                     /translation="LDKIRRQNRIKAELQAVLDEK"
     gene            196..408
                     /locus_tag="KLF07_004638"
     CDS             196..408
                     /locus_tag="KLF07_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBD9834386.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          141 a          101 c          134 g           75 t
ORIGIN      
        1 ctggataaaa tccgccgcca gaaccggata aaggcagaac ttcaggccgt gcttgatgag
       61 aaataaaaaa aagcctccat cagagaggag gcagggaaat aaggcttaaa aggaaataac
      121 ttccgcttcc gaaaggaagc cagtccactg aattatttat cacaataata ctgacaacta
      181 aagggggaca agaaaatgaa tggattcaga aacagttcac ggaacggcca ggtctggcgt
      241 taccagcgtg ccggcgggcg ggccgttatt ctggaagtca gtggacgctg gatggaagcg
      301 gcagaagcat ggcgacgggc tgcctgtgtt gcgcccagga cagactggca gcagtttgcc
      361 cgaaaaaggg ctgaacactg ccaccggcgc tgccggggaa gggtgtaacg aaaaaagcag
      421 ccttcattac tccaggagtc agaaggctga a
//
LOCUS       DAEEBB010000454          451 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.462, whole genome shotgun sequence.
ACCESSION   DAEEBB010000454 DAEEBB010000000
VERSION     DAEEBB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.462"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>451)
                     /gene="ospC2"
                     /locus_tag="KLF07_004639"
     CDS             complement(<1..>451)
                     /gene="ospC2"
                     /locus_tag="KLF07_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBD9834387.1"
                     /translation="QGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTH
                     PLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNTFSEDIE
                     EIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYM"
BASE COUNT          147 a           63 c           89 g          152 t
ORIGIN      
        1 tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca aaatctacag
       61 tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca accccaaaaa
      121 aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt ttatatgttt
      181 gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta tttgacaggt
      241 tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag agcaagtgac
      301 gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag tgtctggaca
      361 ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc gcttcaatag
      421 aaagatttaa tgatttgaaa ctggtccctt g
//
LOCUS       DAEEBB010000455          449 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.463, whole genome shotgun sequence.
ACCESSION   DAEEBB010000455 DAEEBB010000000
VERSION     DAEEBB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.463"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>449
                     /gene="icsB"
                     /locus_tag="KLF07_004640"
     CDS             <1..>449
                     /gene="icsB"
                     /locus_tag="KLF07_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBD9834388.1"
                     /translation="ITHLGHTSISIKNNLLQEPTHTYLSWYPGGNVTKDTEINYLFEK
                     RSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLLNSKQDQNNNKKIFFPRANQKKDP
                     YGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDETNMSKFICQKKA"
BASE COUNT          186 a           83 c           72 g          108 t
ORIGIN      
        1 gcataaccca tttagggcat accagtatca gtataaaaaa caatttacta caggaaccaa
       61 ctcataccta tctcagctgg tatcccggcg ggaatgttac aaaagataca gaaataaact
      121 acctttttga aaaacgctca ggttacagtg tcgataccta taaacaagac aaactaaata
      181 tgatttcaga ccaaacagcc gaaagacttg atgcaggcca agaggttaga aacttattaa
      241 attcaaaaca agatcaaaat aacaacaaaa aaatattttt tcccagagct aatcaaaaaa
      301 aagaccccta tggttattgg ggggtcagtg ctgataaagt atacattcca ttatcaggtg
      361 ataataaaac aaaggatggt aaaatcagct ataacctttt tggccttgat gaaacaaaca
      421 tgtcaaaatt tatatgccaa aagaaagca
//
LOCUS       DAEEBB010000456          445 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.464, whole genome shotgun sequence.
ACCESSION   DAEEBB010000456 DAEEBB010000000
VERSION     DAEEBB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.464"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>445)
                     /locus_tag="KLF07_004641"
     CDS             complement(<1..>445)
                     /locus_tag="KLF07_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HBD9834389.1"
                     /translation="FLYKATSPSSTEYSELKSKISDIHSSQTSLKTPASVSEKESFAT
                     SFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLVHQNGLNFENEK
                     GLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLA"
BASE COUNT          127 a           69 c           85 g          164 t
ORIGIN      
        1 gcaagtctgt gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat
       61 ggtccaaagg cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg
      121 acaatttttt gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag
      181 tacaaaacat ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa
      241 taaatgcttc ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc
      301 tgattaaaag acgttgcaaa gctttctttt tcagacactg atgctggtgt ttttagagaa
      361 gtttgcgaac tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat
      421 ggtgaagttg ccttatataa gaatg
//
LOCUS       DAEEBB010000457          444 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.465, whole genome shotgun sequence.
ACCESSION   DAEEBB010000457 DAEEBB010000000
VERSION     DAEEBB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.465"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>444
                     /locus_tag="KLF07_004642"
     rRNA            <1..>444
                     /locus_tag="KLF07_004642"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAEEBB010000458          442 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.466, whole genome shotgun sequence.
ACCESSION   DAEEBB010000458 DAEEBB010000000
VERSION     DAEEBB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.466"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>442
                     /locus_tag="KLF07_004643"
     CDS             <1..>442
                     /locus_tag="KLF07_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBD9834390.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAEEBB010000459          420 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.467, whole genome shotgun sequence.
ACCESSION   DAEEBB010000459 DAEEBB010000000
VERSION     DAEEBB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.467"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            41..116
                     /locus_tag="KLF07_004644"
     tRNA            41..116
                     /locus_tag="KLF07_004644"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAEEBB010000460          408 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.468, whole genome shotgun sequence.
ACCESSION   DAEEBB010000460 DAEEBB010000000
VERSION     DAEEBB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.468"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
BASE COUNT          125 a           84 c           75 g          124 t
ORIGIN      
        1 acgctgaaaa atgaaagcct gagccactat cgttttaaga gccgggatat ttcgtcagca
       61 tacgggaaaa ctgattgagg tcggcctcag ttttgaggcc tttagccagt tcagccgcaa
      121 gggctttaag cttcttttcg tccataattt acctgtctcc gttgttggag taaacatatc
      181 aaaaacaggc aattacacaa tttaaattac agtctcaaaa caaacccaac ggtcaatata
      241 gtttaccagc aagcttacta gcaaagtgct aatcatttgg tatctttcgt gctattctcc
      301 ttttacgtaa gtacttaagg ggaacgtatt tactactttt aaagaaagct ccgttactat
      361 ctgaaatgca atgagataat aaacgattac tctcggttag tatctcat
//
LOCUS       DAEEBB010000461          408 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.469, whole genome shotgun sequence.
ACCESSION   DAEEBB010000461 DAEEBB010000000
VERSION     DAEEBB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.469"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            54..130
                     /locus_tag="KLF07_004645"
     tRNA            54..130
                     /locus_tag="KLF07_004645"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="KLF07_004646"
     tRNA            173..248
                     /locus_tag="KLF07_004646"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAEEBB010000462          398 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.470, whole genome shotgun sequence.
ACCESSION   DAEEBB010000462 DAEEBB010000000
VERSION     DAEEBB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.470"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..363)
                     /locus_tag="KLF07_004647"
     CDS             complement(<1..363)
                     /locus_tag="KLF07_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBD9834391.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAEEBB010000463          397 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.471, whole genome shotgun sequence.
ACCESSION   DAEEBB010000463 DAEEBB010000000
VERSION     DAEEBB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.471"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>397
                     /locus_tag="KLF07_004648"
     CDS             <1..>397
                     /locus_tag="KLF07_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBD9834392.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          104 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatg
//
LOCUS       DAEEBB010000464          396 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.472, whole genome shotgun sequence.
ACCESSION   DAEEBB010000464 DAEEBB010000000
VERSION     DAEEBB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.472"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..224
                     /locus_tag="KLF07_004649"
     CDS             <1..224
                     /locus_tag="KLF07_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD9834393.1"
                     /translation="RNLTRERTQVGLQAARARGRKGGRPKTLSKDKQALAVQLYNEKK
                     HTVAQICVLMGISRPTLYKYIESARLFKK"
     misc_feature    complement(275..391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          114 a           94 c          105 g           83 t
ORIGIN      
        1 aacgcaacct gacaagagag agaactcagg tcgggcttca agcagcacga gccagaggac
       61 gtaagggcgg caggccgaaa acactgagta aggataaaca ggcccttgct gttcaactct
      121 ataacgagaa aaaacataca gttgcgcaga tttgtgtgtt gatggggata tcgaggccaa
      181 cgctgtacaa atacatagag tcagccaggt tgttcaagaa atgaacttag cgtacgtttt
      241 cgtacggttg ggcttcaaat ccctaatacg ttgacggggc aatatgcagt tcgctgcata
      301 gcggtccgac cccgtactgc tcacgcagct tatccagcag tggcatcatt ttttccagag
      361 gcggtcgaac tccgccttcg caaaataagc ggaagc
//
LOCUS       DAEEBB010000465          394 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.473, whole genome shotgun sequence.
ACCESSION   DAEEBB010000465 DAEEBB010000000
VERSION     DAEEBB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.473"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            167..>394
                     /locus_tag="KLF07_004650"
     CDS             167..>394
                     /locus_tag="KLF07_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBD9834394.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT          118 a           89 c           88 g           99 t
ORIGIN      
        1 aacgttgggt ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga
       61 gtgtccgtta aactcaggct acctcacata gctgattatt aaaaatcaac cacacccgcc
      121 agtattctgt atatttactg gcggtcatat cgtgagaggt atggcaatga atcttgtgac
      181 actcaaaacg tggggaaaac tcagatatcc ggataaccca ccatcaatat caacgctgag
      241 acgatgggca aggaatggaa acatttatcc tgcacctgaa ctacacggga ggagttacag
      301 ggtggttccg gaggctttct atatcaaccc aaataaggtt gataccgata taacacacca
      361 tcagcctaat gggcgacaag ggagagacag tccg
//
LOCUS       DAEEBB010000466          393 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.474, whole genome shotgun sequence.
ACCESSION   DAEEBB010000466 DAEEBB010000000
VERSION     DAEEBB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.474"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            63..>393
                     /gene="yhaV"
                     /locus_tag="KLF07_004651"
     CDS             63..>393
                     /gene="yhaV"
                     /locus_tag="KLF07_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBD9834395.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          101 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat gga
//
LOCUS       DAEEBB010000467          390 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.475, whole genome shotgun sequence.
ACCESSION   DAEEBB010000467 DAEEBB010000000
VERSION     DAEEBB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.475"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>390)
                     /locus_tag="KLF07_004652"
     CDS             complement(<1..>390)
                     /locus_tag="KLF07_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD9834396.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          120 a           78 c           82 g          110 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt
//
LOCUS       DAEEBB010000468          389 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.476, whole genome shotgun sequence.
ACCESSION   DAEEBB010000468 DAEEBB010000000
VERSION     DAEEBB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.476"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..>389
                     /locus_tag="KLF07_004653"
     CDS             <1..>389
                     /locus_tag="KLF07_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834397.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTAA"
BASE COUNT           82 a           99 c          103 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccgcag
//
LOCUS       DAEEBB010000469          389 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.477, whole genome shotgun sequence.
ACCESSION   DAEEBB010000469 DAEEBB010000000
VERSION     DAEEBB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.477"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            251..>389
                     /locus_tag="KLF07_004654"
     CDS             251..>389
                     /locus_tag="KLF07_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBD9834398.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAEEBB010000470          387 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.478, whole genome shotgun sequence.
ACCESSION   DAEEBB010000470 DAEEBB010000000
VERSION     DAEEBB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.478"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..>387)
                     /locus_tag="KLF07_004655"
     CDS             complement(<1..>387)
                     /locus_tag="KLF07_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBD9834399.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           71 a          133 c          113 g           70 t
ORIGIN      
        1 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
       61 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      121 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      181 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      241 acgccgccgc gcaccagcca gagtgattcg ctgcggctgt aaccgtcctc accgggaaac
      301 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      361 agctgtaagc ggatatccgc aggcatt
//
LOCUS       DAEEBB010000471          381 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.479, whole genome shotgun sequence.
ACCESSION   DAEEBB010000471 DAEEBB010000000
VERSION     DAEEBB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.479"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(<1..356)
                     /locus_tag="KLF07_004656"
     CDS             complement(<1..356)
                     /locus_tag="KLF07_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBD9834400.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          101 a           95 c          112 g           73 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac a
//
LOCUS       DAEEBB010000472          381 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.480, whole genome shotgun sequence.
ACCESSION   DAEEBB010000472 DAEEBB010000000
VERSION     DAEEBB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.480"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..241)
                     /locus_tag="KLF07_004657"
                     /pseudo
     CDS             complement(1..241)
                     /locus_tag="KLF07_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a           97 c           88 g           96 t
ORIGIN      
        1 tgtagcgacg acgacggtta cgactgacga caagctgttc ctccaccata agtctgcgta
       61 caaccttttc tgatagccgc cccccctcat gcctgagcat cgcatgcagg cgacgatagc
      121 cataacactg gtaattactg ttaaagatat ctgtcagcat cttacgtatc gtggcgtatt
      181 tatcaccggc tttcagtgca gcccggtgat aaaaataact gctacgggca aggcccagaa
      241 ctgaggtgta ctggcaatag cggacaccac catttgttct ttttctaagc agtcatctga
      301 taatatttta tcctgaaggc tgccggggag atattcccca gacgagagtg acgacgctga
      361 cgattgtaga aaatctcaat g
//
LOCUS       DAEEBB010000473          368 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.denovo.481, whole genome shotgun sequence.
ACCESSION   DAEEBB010000473 DAEEBB010000000
VERSION     DAEEBB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.denovo.481"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
BASE COUNT           69 a          132 c          116 g           51 t
ORIGIN      
        1 acctgagaag aggaaacaga acggggactg gccggggaag ataaggaaga ggcgcgctgt
       61 ttacgggacg cttccgcccg ctgcgcctgc gtgctcagcg ccatgtgaaa acggcggccc
      121 gcgccgtccg tcacccccgt caccgcgccc gccacgtcac cctccgccgc ccggtggaaa
      181 gtcagtgagc gcccgaagcc atccaccacg accgtcagta cccggtatgc gggcggctcc
      241 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      301 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc ttagcggctg cgaactgtgc
      361 tgcgccgc
//
LOCUS       DAEEBB010000474         3895 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.251, whole genome shotgun sequence.
ACCESSION   DAEEBB010000474 DAEEBB010000000
VERSION     DAEEBB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.251"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="KLF07_004658"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="KLF07_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBD9834401.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="KLF07_004659"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="KLF07_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBD9834402.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="KLF07_004660"
     CDS             2675..3511
                     /locus_tag="KLF07_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD9834403.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..>3895
                     /locus_tag="KLF07_004661"
     CDS             3817..>3895
                     /locus_tag="KLF07_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBD9834404.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAH"
BASE COUNT          836 a         1101 c         1077 g          881 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacg
//
LOCUS       DAEEBB010000475         3855 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.255, whole genome shotgun sequence.
ACCESSION   DAEEBB010000475 DAEEBB010000000
VERSION     DAEEBB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.255"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(1..817)
                     /locus_tag="KLF07_004662"
                     /pseudo
     CDS             complement(1..817)
                     /locus_tag="KLF07_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1212..1868
                     /locus_tag="KLF07_004663"
     CDS             1212..1868
                     /locus_tag="KLF07_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HBD9834405.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(2187..2603)
                     /locus_tag="KLF07_004664"
     CDS             complement(2187..2603)
                     /locus_tag="KLF07_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD9834406.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(2648..>3855)
                     /locus_tag="KLF07_004665"
     CDS             complement(2648..>3855)
                     /locus_tag="KLF07_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HBD9834407.1"
                     /translation="HHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCRCEES
                     DTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNASTVRNH
                     LCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRNFEII
                     AGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADNLRDL
                     QFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLWHGNV
                     VAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRYGEPV
                     STAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYPGFQS
                     DGKGPAMAA"
BASE COUNT          932 a         1013 c          910 g         1000 t
ORIGIN      
        1 gtgctcgctg cccagtcaat gatctccctg tcgcagcagt cctgcgcgaa ggtcacccgc
       61 agcttttcgc cattatcaca gcggaactca aagccatctg agcaccagcg ccggttactt
      121 tccgctactg ctacgcgccc cttatgggcc cgcttacggc aaggatcagc gggtttacgt
      181 tcaagaagca gattatgcgt tctcataatg cgatacaccc gctttgcatt aaccacaggc
      241 aggccgtccc gctccgactc ccgtcgcagc agcgcccaca cacggcgata accatacgtc
      301 ggcaggtcag ccaccgccat gtttatccgg gacaatacgg tggtatcgtc agaacggggc
      361 tgccgtctgc gatcctgtca gtcagatggc cggtgaaccc gaatgctcaa ctgcgcacgc
      421 gacacgccaa gacttctgca aacgtcgctt attcgtcgtt cccaggcaac gagggcgcat
      481 gcgcaatcca tttttttgcc cggccgaact ccacggcctc tttgagtatc tcggcttcca
      541 tcgtcttttt gcccagaagg cgctggagtt cgcgaatttg cttattggcg gcagcgagtt
      601 cggaggccgg caccacttcc tcgcccgatg caacagccgt cagtgaaccg tcttaatatt
      661 gcttgcgcca tttgaatatc tggtttgcat tgatgccatg caggcgcgcg acatgagaca
      721 cggtcatacc gggctccata gtctgctgaa taatggcaat tttttcctgc ggtgtacgac
      781 gccgacgccg ctcaggtcct gataacactt caaccatctt atcgttctga ctggtattaa
      841 acatagttcc aagactacct cttattttaa gagagtcgaa gtgtctggtg atctatgggg
      901 ccagtctagc cctcttccaa gtgtagggag atatagtcaa agtttgcttc tccgccgatg
      961 agtccgccct gcgtcggata gtccgatttt ttaccggcga agcacggctc tcctaaccct
     1021 atctaaagcg aataactttt ttcaacaggg ccgcgtgcca atgcaagagc gataggcgct
     1081 ctacgcgcca atttgaccac ttaaaacagg taaattgagg ggttgtaatg tgttgatgta
     1141 acaggctttt attttaatgt cttggcatat gtataatggt agtctagccc tcctttcaac
     1201 aaggagtact catggaaacc tacaatcata catatcggca ccacaacttt tcacataaag
     1261 acttaagtga tctcaccttc accgcttgca cattcattcg cagcgacttt cgacgtgcta
     1321 acttgcgtga tacgacattc gtcaactgca agttcattga acagggtgat atcgaaggct
     1381 gccactttga tgtcgcagat cttcgtgatg caagtttcca acaatgccaa cttgcgatgg
     1441 caaacttcag taatgccaat tgctacggta tagagttccg tgcgtgtgat ttaaaaggtg
     1501 ccaacttttc ccgaacaaac tttgcccatc aagtgagtaa tcgtatgtac ttttgctcag
     1561 catttatttc tggatgtaat ctttcctatg ccaatatgga gagggtttgt ttagaaaaat
     1621 gtgagttgtt tgaaaatcgc tggataggaa cgaacctagc gggtgcatca ctgaaagagt
     1681 cagacttaag tcgaggtgtt ttttccgaag atgtctgggg gcaatttagc ctacagggtg
     1741 ccaatttatg ccacgccgaa ctcgacggtt tagatccccg caaagtcgat acatcaggta
     1801 tcaaaattgc agcctggcag caagaactga ttctcgaagc actgggtatt gttgtttatc
     1861 ctgactaatt gctttgatgt gtgattttaa acgctcaaat ttataaaacg aataatattg
     1921 tcaaccttgc aataatagac atagggacaa ccatgtataa atttttaccc tcaggcgtct
     1981 tcacaataga gccatatagc agtgggtcac ttagtgggct taatttttcc attaaagata
     2041 atattaatat tgctcagtat aaaacatcat atggtagccc atcttggcaa gtaaacataa
     2101 ggctgccatt tataaggcac tgttgcaaaa atgtggaggt gataggggta ctacgccgga
     2161 accccagatt tttccgtccg ttcaaattat ccacggttga gcagcttgat cccgtccgcc
     2221 cgcagttgac caacagggga aatatgcctg tacaaagttt gccgggttat cccaagttcc
     2281 tggcatagcg tactgacctt tgtttctgat tgtcccattg atgccattgc cagtcgcagt
     2341 ttgactggcg tcattttata aggtcggccg ccgttccgcc cacgggctct tgctgatgcc
     2401 agacctgcag tcgttctttc agcaatcagt tcgcgctcaa actccgccag tgcggcaaag
     2461 ataccgaaga caagcttgcc agcggccgtt gttgtgtcga tagtcgcccc gtgaccggtc
     2521 agcactttca gaccggtccc cctcgcggtc aggtcgtgca ctgtattaat gagatgacga
     2581 agatcacgac cgaggcggtc cagcttccat acgaccgaga gcataaaaaa tattggggca
     2641 gcgtgagcta tgctgccatc gctggtccct tcccgtcact ttgaaaaccc ggataccagc
     2701 agacaaattt atcctgcagt tcgttattca aaacggcggt ccgggtctgt aacaaataat
     2761 gagccccttt tctgctccac tgcatctgct gctttttggc cattcgtctg gcgatcactt
     2821 cattgatcgt ggattccaca aacgcggttg ataccggctc tccgtaccga cgcatttcgc
     2881 cgtagtttgg gatcatcatc ttattattcc ggatgtaggt atacatttca tccagatgtt
     2941 tttgcaggga tttcaggcta ggatagctga gttcagggtc atcgcaatac ataacgcaat
     3001 tatcaatatg ttccagcgca gcgacaacat tgccatgcca gagatatcgt ttaatgcttt
     3061 ccagcagtgc cagaacttta ctccccgcct ctggatctga taccagcagt ccccgggcat
     3121 attgcatgag caccttcagc ctcatggtga tatgaaacca gtccagcaca tgcgttgact
     3181 cagggtacat accgaactgg aggtccctga gattatccgc gccgtcggac agaaaaaata
     3241 tctgctggtt ggcctgcatt ccctgcgctg aaaggtgggt catgagcctg cgttccggat
     3301 gacagtcatc cttctgaaca aaaccgaagc ggcgggtgtc agcaggcgca ccaacggaaa
     3361 aagacttccc ggcaataatt tcaaaattac gctttttgtc atcccgatcg cgaacataac
     3421 caccatctat acccaccaca agcggttttc ctggcctggg caggttcccc cagtcgcggg
     3481 ggcagccgga aagaaaacct gaatgagctt cagcctcagc gtcaagacgc tgcgccacct
     3541 ggcataaatg attcctcacc gttgaggcat tcaggctgtg gcccaccggc agaatatctt
     3601 tcagcagacg agtcgtcatt tcataggaga tcatcgaggc ccagcgggtt tcaatatatt
     3661 tcagtgccgg gtgagaataa tcgccggccc agtcgctgag caaactgact gtctttgtat
     3721 cactctcttc acaacggcac cggtacaccc gaagcccgga taccggaata acgccaaaca
     3781 gcgtccggta ccgtattttc tgtttgcctt tgattctgcg cgcagcaagg cagtgagggc
     3841 accggatatg gtgtt
//
LOCUS       DAEEBB010000476         3850 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.256, whole genome shotgun sequence.
ACCESSION   DAEEBB010000476 DAEEBB010000000
VERSION     DAEEBB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.256"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..204
                     /locus_tag="KLF07_004666"
     CDS             <1..204
                     /locus_tag="KLF07_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBD9834408.1"
                     /translation="AHEAGARTRATAHERTPQQERQKAAREAERGREAWTLGQGMKKP
                     VAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(236..886)
                     /gene="tetR(A)"
                     /locus_tag="KLF07_004667"
     CDS             complement(236..886)
                     /gene="tetR(A)"
                     /locus_tag="KLF07_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBD9834409.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            992..2191
                     /gene="tet(A)"
                     /locus_tag="KLF07_004668"
     CDS             992..2191
                     /gene="tet(A)"
                     /locus_tag="KLF07_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBD9834410.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2158..2346)
                     /locus_tag="KLF07_004669"
                     /pseudo
     CDS             complement(2158..2346)
                     /locus_tag="KLF07_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2390..2779)
                     /locus_tag="KLF07_004670"
     CDS             complement(2390..2779)
                     /locus_tag="KLF07_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD9834411.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2997..3437
                     /locus_tag="KLF07_004671"
     CDS             2997..3437
                     /locus_tag="KLF07_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBD9834412.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          810 a         1105 c         1094 g          841 t
ORIGIN      
        1 gcgcacgagg ccggcgcgcg gacccgcgcc acggcccacg agcggacgcc gcagcaggag
       61 cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg cttggacgct agggcagggc
      121 atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat
      181 gttgtctaca tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat
      241 cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat
      301 cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt
      361 ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc
      421 cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg
      481 tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct
      541 gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc
      601 catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc
      661 gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg
      721 ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa
      781 cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca
      841 ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat
      901 cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc
      961 ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat
     1021 tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg
     1081 cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc
     1141 gctgtatgcg ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt
     1201 cgggcggcgg ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact acgccatcat
     1261 ggcgacggcg cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg
     1321 ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc
     1381 gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct
     1441 cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa
     1501 cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg
     1561 gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac
     1621 cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc
     1681 cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat
     1741 ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt
     1801 agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg
     1861 ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct
     1921 tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga
     1981 ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt
     2041 cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc
     2101 atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg
     2161 gagaaattct tcaaattccc gttgcacata gcccggcaat tcctttccct gctctgccat
     2221 aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg tttgctcggg
     2281 tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg ccgccacatg
     2341 aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct tagcggaaag
     2401 ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca gtgcccgtca
     2461 ctcccactct tacattgttc gcagtatcta ggtacatgtt attgcgtgca ccttcatgat
     2521 gtataatgtt attaatgaca gcagaaagat cgacagaatt aatattcttc gtacctggga
     2581 cagttatcgg gtgtcggcca gtgatgagac caaaggatgc agtataagat gaaccactac
     2641 tattatcatg gccaccatta ccaccaccgt taaaactacc tcccgaactt ccagagccgc
     2701 tatgaccatc attgtagggg tcatttggtc ccatgtgacc aaaaccaccg acagtaccgc
     2761 tatttaactc tgtactcata aaattatcct ctttgacttt taaaaacagc aaaaactgta
     2821 tttataacaa tgtgtttata tacagtattc gaaatactac gcccgtcaaa accacaccgt
     2881 caacaggttt tttgtgtatt tttcacgcct cacgggcagc ctcagacctt ttgtgttaaa
     2941 acagtaatat gttctgctgt tacaatacgg tacacacagg agacactgtc acttttatgg
     3001 catggcacac agttcaagag acactgaaat taacaggaaa gtcgcgctcg caattatatc
     3061 gtgacatggc taaaggcctt gtcagctatc gcacaggaaa aaatgatcgc cgggagttcg
     3121 agaccagtga actgattcgg gcctatggcg agctgcgcac ttctgagaca ccaaaaaggc
     3181 acaaggaggg acactccaaa tctgtcgtta gccaggcgga aaatgctcag ctagacgtca
     3241 ttcagcagaa actggacgct ctgcagcaga ctgttaccct aatgcttgaa gacaagatgg
     3301 cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga acaactacag gccgaaatta
     3361 gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg attctggtcg cggctgtttc
     3421 gctccggttc agagtaacag tgcaggtgac tctgatttac cataacatct gttatcagca
     3481 ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg gtaaaacagg caacaaacca
     3541 cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga gtatgcgcac
     3601 aagtgcatag cgcggaaatt cacaccattc agccggggta agtcctgttt ccggcgttct
     3661 tcccatccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa gcgttgccgg
     3721 cgcagccgga aaacccgctg tcagcacgtt caggaagggg gtgatgtaca aatggcacag
     3781 aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa gtgatgtata agtgatgtaa
     3841 aagtgatgta
//
LOCUS       DAEEBB010000477         3564 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.263, whole genome shotgun sequence.
ACCESSION   DAEEBB010000477 DAEEBB010000000
VERSION     DAEEBB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.263"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            561..800
                     /locus_tag="KLF07_004672"
     CDS             561..800
                     /locus_tag="KLF07_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBD9834413.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="KLF07_004673"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="KLF07_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /gene="dfrA"
                     /locus_tag="KLF07_004674"
     CDS             2297..2770
                     /gene="dfrA"
                     /locus_tag="KLF07_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HBD9834414.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="KLF07_004675"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="KLF07_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HBD9834415.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT         1020 a          757 c          801 g          986 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctagaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt
     3481 tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt
     3541 agtggatgaa gctcgtcttc ccta
//
LOCUS       DAEEBB010000478         2485 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.297, whole genome shotgun sequence.
ACCESSION   DAEEBB010000478 DAEEBB010000000
VERSION     DAEEBB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.297"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            complement(214..381)
                     /locus_tag="KLF07_004676"
                     /pseudo
     CDS             complement(214..381)
                     /locus_tag="KLF07_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            530..1405
                     /locus_tag="KLF07_004677"
     CDS             530..1405
                     /locus_tag="KLF07_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-15"
                     /protein_id="HBD9834416.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(1452..1784)
                     /locus_tag="KLF07_004678"
     CDS             complement(1452..1784)
                     /locus_tag="KLF07_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBD9834417.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            1798..2485
                     /locus_tag="KLF07_004679"
                     /pseudo
     CDS             1798..2485
                     /locus_tag="KLF07_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT          621 a          618 c          652 g          594 t
ORIGIN      
        1 agataacaag ataacaagat aacaagataa caccttgtta tcttgttatc ttgttatctt
       61 gttatcgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      361 acgctcaccg gctccagatt taaggtggtt gtaaataatg ttacaatgtg tgagaagcag
      421 tctaaattct tcgtgaaata gtgatttttg aagctaataa aaaacacacg tggaatttag
      481 ggactattca tgttgttgtt atttcgtatc ttccagaata aggaatccca tggttaaaaa
      541 atcactgcgc cagttcacgc tgatggcgac ggcaaccgtc acgctgttgt taggaagtgt
      601 gccgctgtat gcgcaaacgg cggacgtaca gcaaaaactt gccgaattag agcggcagtc
      661 gggaggcaga ctgggtgtgg cattgattaa cacagcagat aattcgcaaa tactttatcg
      721 tgctgatgag cgctttgcga tgtgcagcac cagtaaagtg atggccgcgg ccgcggtgct
      781 gaagaaaagt gaaagcgaac cgaatctgtt aaatcagcga gttgagatca aaaaatctga
      841 ccttgttaac tataatccga ttgcggaaaa gcacgtcaat gggacgatgt cactggctga
      901 gcttagcgcg gccgcgctac agtacagcga taacgtggcg atgaataagc tgattgctca
      961 cgttggcggc ccggctagcg tcaccgcgtt cgcccgacag ctgggagacg aaacgttccg
     1021 tctcgaccgt accgagccga cgttaaacac cgccattccg ggcgatccgc gtgataccac
     1081 ttcacctcgg gcaatggcgc aaactctgcg gaatctgacg ctgggtaaag cattgggcga
     1141 cagccaacgg gcgcagctgg tgacatggat gaaaggcaat accaccggtg cagcgagcat
     1201 tcaggctgga ctgcctgctt cctgggttgt gggggataaa accggcagcg gtggctatgg
     1261 caccaccaac gatatcgcgg tgatctggcc aaaagatcgt gcgccgctga ttctggtcac
     1321 ttacttcacc cagcctcaac ctaaggcaga aagccgtcgc gatgtattag cgtcggcggc
     1381 taaaatcgtc accgacggtt tgtaatagcg gaaacggaat ggggaaactc attccgtttt
     1441 tgtttatcgc cttagacggc aaaagtgctg tcgcccacct gcgcttgcgc ataccaggcc
     1501 ataagctccg tggttcctgg ttctccttcc gctggagccc agtgcgcata gtcatcggca
     1561 gccacgggtt gatagccacc gtgttttact tcaaaaatta tgccaccggt atccagcgac
     1621 agcacggcat gccaggttcc tgcggccatc tccagcaccg tacaggtttc ccccaatatc
     1681 gcccgatggg tgacggtacc ccgatcgtca aaattcagca ccacgaaacg accccttaat
     1741 ggcaacagta gctcgaaggt gtgagggtgt cggtgcgggc gcacgtaggt cccaggtcat
     1801 attccttccg gcgtccggca ttttaccgcc agacagctcg ggattcgtga tatcaccgtt
     1861 cttgcagaat acggtcagag ggaaaatacc cgccgtgagc atgcagcgct gatacgtcag
     1921 cactatcagt atcgtgaatt tgcctggccc tggacatttc gccttacccg tcttttatat
     1981 acccggagct ggataagcaa cgaacgtcct ggcctgcttt tcgatctggc gacagggtgg
     2041 cttatgcaac atcgtattat tctccccgga gccactacgc tgacccggtt gatttcagag
     2101 gtaagggaaa aggcgacgtt gcgcctgtgg aacaaactgg cactgatacc gtcagccgaa
     2161 cagcgttcac agctggagat gctgctgggg ccaactgatt gcagccgcct gtctttactg
     2221 gaatcactga aaaagggccc tgtgaccatc agtggtccgg cgtttaatga agcaattgaa
     2281 cgctggaaaa ctctgaacga ttttggcctg catgctgaaa acctgagtac actcccggct
     2341 gtgcgcctga aaaatctcgc acgttatgct ggtatgactt cggtgttcaa tattgccagg
     2401 atgtcaccgc agaaaaggat ggcggttctg gttgcctttg tccttgcatg ggaaacgctg
     2461 gcgctggatg atgcattgga cgttc
//
LOCUS       DAEEBB010000479         2146 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.306, whole genome shotgun sequence.
ACCESSION   DAEEBB010000479 DAEEBB010000000
VERSION     DAEEBB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.306"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            <1..162
                     /locus_tag="KLF07_004680"
     CDS             <1..162
                     /locus_tag="KLF07_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HBD9834418.1"
                     /translation="RMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKD
                     FLIATLKPR"
     gene            173..568
                     /gene="frdC"
                     /locus_tag="KLF07_004681"
     CDS             173..568
                     /gene="frdC"
                     /locus_tag="KLF07_004681"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD9834419.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            579..938
                     /gene="frdD"
                     /locus_tag="KLF07_004682"
     CDS             579..938
                     /gene="frdD"
                     /locus_tag="KLF07_004682"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD9834420.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1001..2134
                     /locus_tag="KLF07_004683"
     CDS             1001..2134
                     /locus_tag="KLF07_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD9834421.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          526 a          540 c          561 g          519 t
ORIGIN      
        1 cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt gggctattgc
       61 tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa
      121 agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac
      181 taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta
      241 tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact
      301 gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt
      361 tttacaaaac ccggttatcg tgatcattaa cctgatcact ctggcggcag ctctgctgca
      421 caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa
      481 aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt
      541 aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc
      601 gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta
      661 ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg
      721 atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc
      781 tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc
      841 acgatctgaa aattcacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc
      901 tgacagttgt cacgctgatt ggtatcgtta caatctaacg tatcgccaat gtaaatccgg
      961 cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg
     1021 cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat
     1081 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
     1141 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
     1201 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
     1261 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa
     1321 atactggcct gaacttactg ctaaacagtg gaatgggatc acactattac atcttgcgac
     1381 ctacaccgct ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt
     1441 gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc
     1501 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
     1561 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
     1621 acctccccca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt
     1681 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
     1741 ccgctgggtg caaagcaatt taacccccct tgatatcaat gagaaaacgc ttcaacaagg
     1801 gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg
     1861 ggaaatgctg gactggccgg tcaatcctga cagcatcatt aacggcagtg acaataaaat
     1921 tgcactggca gcacgcccgg taaaagcgat taagccccca actcctgcag tacgcgcatc
     1981 atgggtacat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga
     2041 aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagtcgc
     2101 cgccgcctgt cagattctca acgctctaca gtaaaatttc atcggg
//
LOCUS       DAEEBB010000480         1166 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei isolate clinical
            SAMEA8562909-rid12257263.guided.374, whole genome shotgun sequence.
ACCESSION   DAEEBB010000480 DAEEBB010000000
VERSION     DAEEBB010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8562909
            Sequence Read Archive: ERR5708694
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043083.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043083.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2021 18:48:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,685
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,278
            CDSs (with protein)               :: 4,278
            Genes (RNA)                       :: 92
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 315
            CDSs (without protein)            :: 315
            Pseudo Genes (ambiguous residues) :: 0 of 315
            Pseudo Genes (frameshifted)       :: 144 of 315
            Pseudo Genes (incomplete)         :: 111 of 315
            Pseudo Genes (internal stop)      :: 105 of 315
            Pseudo Genes (multiple problems)  :: 44 of 315
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8562909-rid12257263.guided.374"
                     /isolate="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Belgium"
                     /collection_date="2015-03-28"
                     /collected_by="NRCSS Belgium at Sciensano"
     gene            135..593
                     /gene="gpt"
                     /locus_tag="KLF07_004684"
     CDS             135..593
                     /gene="gpt"
                     /locus_tag="KLF07_004684"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD9834422.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            685..>1166
                     /locus_tag="KLF07_004685"
     CDS             685..>1166
                     /locus_tag="KLF07_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD9834423.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     A"
BASE COUNT          279 a          298 c          305 g          284 t
ORIGIN      
        1 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
       61 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
      121 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
      181 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
      241 gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
      301 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctaaaac
      361 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
      421 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
      481 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
      541 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
      601 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
      661 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
      721 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
      781 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
      841 cgtctgatgt ggatctggcg cggcattgac ccacgtgaaa tcctcgacgt ccaggcgcgt
      901 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
      961 cgtggtggca actggattta tgagtgggcc atccaggcga tggtgtggca acaaaaagcc
     1021 tgtgcggaag aagatccgca actcagtggt cgtcactggc tgcatgcggc tacgttgtac
     1081 aacattgctg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggctttg
     1141 tcaaaccgtg cctatgaaga agccgc
//
